LOCUS       NM_001136030            4166 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens thymocyte expressed, positive selection associated 1
            (TESPA1), transcript variant 1, mRNA.
ACCESSION   NM_001136030
VERSION     NM_001136030.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Jung,J., McCartney,D.L., Wagner,J., Rosoff,D.B., Schwandt,M.,
            Sun,H., Wiers,C.E., de Carvalho,L.M., Volkow,N.D., Walker,R.M.,
            Campbell,A., Porteous,D.J., McIntosh,A.M., Marioni,R.E.,
            Horvath,S., Evans,K.L. and Lohoff,F.W.
  TITLE     Alcohol use disorder is associated with DNA methylation-based
            shortening of telomere length and regulated by TESPA1: implications
            for aging
  JOURNAL   Mol Psychiatry 27 (9), 3875-3884 (2022)
   PUBMED   35705636
  REMARK    GeneRIF: Alcohol use disorder is associated with DNA
            methylation-based shortening of telomere length and regulated by
            TESPA1: implications for aging.
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Lyu,J., Wang,P., Xu,T., Shen,Y., Cui,Z., Zheng,M., Fu,G. and Lu,L.
  TITLE     Thymic-specific regulation of TCR signaling by Tespa1
  JOURNAL   Cell Mol Immunol 16 (12), 897-907 (2019)
   PUBMED   31316154
  REMARK    GeneRIF: Thymic-specific regulation of TCR signaling by Tespa1.
REFERENCE   3  (bases 1 to 4166)
  AUTHORS   Liang,J., Lyu,J., Zhao,M., Li,D., Zheng,M., Fang,Y., Zhao,F.,
            Lou,J., Guo,C., Wang,L., Wang,D., Liu,W. and Lu,L.
  TITLE     Tespa1 regulates T cell receptor-induced calcium signals by
            recruiting inositol 1,4,5-trisphosphate receptors
  JOURNAL   Nat Commun 8, 15732 (2017)
   PUBMED   28598420
  REMARK    GeneRIF: Tespa1 functions in T cell development and the regulation
            of TCR-induced Ca(2+) signalling through IP3R1
            Erratum:[Nat Commun. 2018 Mar 12;9:16183. doi:
            10.1038/ncomms16183.. PMID: 29528040]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4166)
  AUTHORS   Yao,Y., Zhang,H., Shao,S., Cui,G., Zhang,T. and Sun,H.
  TITLE     Tespa1 is associated with susceptibility but not severity of
            rheumatoid arthritis in the Zhejiang Han population in China
  JOURNAL   Clin Rheumatol 34 (4), 665-671 (2015)
   PUBMED   25736038
  REMARK    GeneRIF: Tespa1 hay have a role in susceptibility but not severity
            of rheumatoid arthritis in the Zhejiang Han population in China
REFERENCE   5  (bases 1 to 4166)
  AUTHORS   Liu,S., Liu,L., Wu,S., Yang,T., Pan,F., Laslett,L., Xia,G., Hu,Y.,
            Fan,D., Ding,N., Xu,S., Cai,G., Wang,L. and Xin,L.
  TITLE     Lack of association between TESPA1 gene polymorphisms (rs1801876,
            rs2171497, rs4758994, and rs997173) and ankylosing spondylitis in a
            Chinese population
  JOURNAL   Inflammation 37 (6), 2040-2046 (2014)
   PUBMED   24893580
  REMARK    GeneRIF: The TESPA1 gene was found to not be involved in ankylosing
            spondylitis in a Chinese population.
REFERENCE   6  (bases 1 to 4166)
  AUTHORS   Fujimoto,T., Matsuzaki,H., Tanaka,M. and Shirasawa,S.
  TITLE     Tespa1 protein is phosphorylated in response to store-operated
            calcium entry
  JOURNAL   Biochem Biophys Res Commun 434 (1), 162-165 (2013)
   PUBMED   23541577
  REMARK    GeneRIF: Tespa1 is post-translationally modified upon intracellular
            divalent calcium-ion Ca2+ increase in thymocytes.
REFERENCE   7  (bases 1 to 4166)
  AUTHORS   Matsuzaki,H., Fujimoto,T., Tanaka,M. and Shirasawa,S.
  TITLE     Tespa1 is a novel component of mitochondria-associated endoplasmic
            reticulum membranes and affects mitochondrial calcium flux
  JOURNAL   Biochem Biophys Res Commun 433 (3), 322-326 (2013)
   PUBMED   23501103
  REMARK    GeneRIF: Tespa1 is a novel component of mitochondria-associated
            endoplasmic reticulum membranes and affects mitochondrial calcium
            flux.
REFERENCE   8  (bases 1 to 4166)
  AUTHORS   Wang,D., Zheng,M., Lei,L., Ji,J., Yao,Y., Qiu,Y., Ma,L., Lou,J.,
            Ouyang,C., Zhang,X., He,Y., Chi,J., Wang,L., Kuang,Y., Wang,J.,
            Cao,X. and Lu,L.
  TITLE     Tespa1 is involved in late thymocyte development through the
            regulation of TCR-mediated signaling
  JOURNAL   Nat Immunol 13 (6), 560-568 (2012)
   PUBMED   22561606
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4166)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC079842.19, AW967924.1 and AK303223.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001136030.2.
            
            Transcript Variant: This variant (1), as well as variants 2 and 6,
            encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.177186.1,
                                           SRR1163658.285329.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000449076.6/ ENSP00000400892.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC079842.19        128073-128088       c
            17-90               AW967924.1         1-74
            91-2100             AK303223.1         1-2010
            2101-4166           AC079842.19        91341-93406         c
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..4166
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="thymocyte expressed, positive selection associated
                     1"
                     /db_xref="GeneID:9840"
                     /db_xref="HGNC:HGNC:29109"
                     /db_xref="MIM:615664"
     exon            1..178
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            179..386
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..1789
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="isoform 1 is encoded by transcript variant 1;
                     thymocyte-expressed positive selection-associated protein
                     1; protein TESPA1"
                     /codon_start=1
                     /product="protein TESPA1 isoform 1"
                     /protein_id="NP_001129502.1"
                     /db_xref="CCDS:CCDS44913.1"
                     /db_xref="GeneID:9840"
                     /db_xref="HGNC:HGNC:29109"
                     /db_xref="MIM:615664"
                     /translation="MEASVLSPTSWEKRRAWLRQSRNWQTQVLEEEAAAALQDVPDPE
                     PSSLDDVFQEGNPINKIEDWLQDCGYSEEGFSEEAGQFIYNGFCSHGTSFEDDLTLGA
                     EATLLAANGKLFSRSFLETARPCQLLDLGCSLASSSMTGGTNKTSSSISEILDKVQED
                     AEDVLFSLGFGQEDHKDTSRIPARFFTTPSQAKGIDFQLFLKSQVRRIEMEDPCLMLA
                     SRFKQVQTLAVTADAFFCLYSYVSKTPVQKFTPSHMFWNCNHPTDVPSIRILSREPEP
                     QSPRDRLRKAISKMCLYTCPRDRPPPPHNTPKRNSLDQVVLEVMDKVKEEKQFLQQDS
                     DLGQFSQEDPVPPAEGKKLPTSPYPCVFCCEEETQQRMSTVLAPSQTLDSNPKVPCCT
                     HSLPIEDPQWSTDPAQIRRELCSLPATNTETHPAKDETFWKRKSRARKSLFQKNLMGR
                     KVKSLDLSITQQKWKQSVDRPELRRSLSQQPQDTFDLEEVQSNSEEEQSQSRWPSRPR
                     HPHHHQTFAGKDS"
     exon            387..429
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            430..479
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            480..533
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            534..570
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            571..669
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            670..878
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1690
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1790
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..4166
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2082..2087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="hexamer: AATATA"
     regulatory      2101..2106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="hexamer: AGTAAA"
     polyA_site      2111
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="major polyA site"
     polyA_site      2122
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
     regulatory      3440..3445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="hexamer: AATAAA"
     polyA_site      3462
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
     regulatory      4140..4145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
                     /note="hexamer: ATTAAA"
     polyA_site      4166
                     /gene="TESPA1"
                     /gene_synonym="HSPC257; ITPRID3; KIAA0748"
ORIGIN      
        1 ctcatttccg cttccccttt tgagcaggca acagcagttg aatgagttca gggagaaagg
       61 cataatctac aacatgatac tttctcactt gcttcacctc tgtgcccaca cctgtgaagg
      121 cctcggccca gcttcgacaa ctgctttgcc tcaaactcag ctttctacca gcagacagga
      181 cggcattact gttacgggag gccctgaagt ctgagccagg gccatggagg cctctgtgct
      241 gagccccaca tcctgggaga aacggcgggc ctggctccgt cagagccgta actggcagac
      301 ccaggtccta gaagaggagg ctgccgccgc cctgcaggat gtcccagatc ctgagccttc
      361 cagcctggat gacgttttcc aagaagggaa tccaatcaat aaaattgaag actggctgca
      421 ggattgcgga tactctgaag aaggattttc tgaggaagca ggacagttta tctacaatgg
      481 cttctgcagc catgggacca gctttgaaga tgacttgacc ctgggagcgg aggccacact
      541 actggccgcc aatggcaaac tcttctccag gagtttcctc gagacagcca ggccttgcca
      601 gctacttgat cttggctgta gtttggcttc cagcagcatg actgggggga ccaacaagac
      661 tagttcaagc atctcagaaa ttctggacaa agtgcaagaa gatgcagaag atgtcctctt
      721 cagtctgggc tttggccaag aggatcacaa agacacttct cggatacccg cccgattttt
      781 caccaccccc tctcaggcca agggcattga tttccagctc ttcctgaagt cccaggtgcg
      841 gaggattgaa atggaagacc cctgcctcat gctggccagc aggtttaagc aggtgcaaac
      901 actggctgtc actgctgatg ccttcttctg tctctactcc tatgtgtcta agacacctgt
      961 ccaaaagttc acaccatccc acatgttctg gaattgcaac catccaactg atgtgccatc
     1021 catcaggatt ctgtcccgag agcctgaacc ccagtcaccc agagaccgcc ttcggaaagc
     1081 catctccaag atgtgtctgt acacatgccc ccgagaccgg ccaccaccac cccacaacac
     1141 ccccaaaagg aacagtttgg accaagttgt gttggaagtg atggacaaag tgaaagaaga
     1201 gaagcagttc ctccagcaag actctgattt ggggcaattc tcacaggaag atcctgtgcc
     1261 ccctgctgag ggtaagaagt tgcccacttc tccctatcca tgtgtcttct gctgtgaaga
     1321 ggaaacacag cagaggatgt ccacagtgct agcaccatcc caaactctgg attcgaaccc
     1381 caaggtaccc tgttgcacac attctctgcc catagaagat ccacagtgga gcacagaccc
     1441 agctcagata aggagagagc tgtgtagtct accagccacc aatacggaaa cccatccagc
     1501 caaggatgag actttctgga aaagaaagag cagagcaaga aagagcctct tccagaagaa
     1561 tctcatgggc aggaaggtta agtccttgga cctgtccatc acccagcaga aatggaagca
     1621 gagtgtagac agaccagaac tgcgtcggtc tttaagccag cagccacagg acacttttga
     1681 cttggaggag gtgcaaagca acagtgagga ggagcagagt cagagtcgct ggcccagcag
     1741 acccaggcac ccccaccacc accagacttt tgctggcaaa gactcgtgaa gctttctcca
     1801 ccaccacaac agcatgtggt ctgacagaat tggcttcaaa gaagaaacta aaagccacct
     1861 cttgcaggaa actgcactgc cctcaacatc cagcccctac tccttggtcc tccaaacccc
     1921 caaactctgc aggagaaagg cagcatggta catggggagg aagaccagac tgagcgattt
     1981 ggaatccaca tcctaatgct gccacaagct gcatgcacaa agaccttagg cacatctctt
     2041 catttctctg tacactggtt tctctactat gtgtgtatta aaatatataa tgtggatgat
     2101 agtaaactga acaaagcctt aattttctcc caagctttga cattgccaag ggcagttagg
     2161 agacttcagg atcaagttta ggggacaagt ttttttctaa tactttcaaa aggcccaagt
     2221 gaagagagga aggacacctc actttctggc tctaaaagca tggtacatct cacactagga
     2281 ttccctctgc acctctgttc atccctgtat tcctaactaa attccagaaa atcttctaca
     2341 acactggagt cctatttttc ttcttgagac ctcctttgaa gaaatcaact tggagactcc
     2401 tccttaaatc cagtttcttt ctctacccaa aattttgcat ctttatagaa aaagagataa
     2461 gtaggattca gagtctcttc tcctagccat tgtgggggga aaaaatgcta tttctgttgg
     2521 cagaaaacaa tacaaatatt ttttttaaat catggcatgt ttttaaagtt aactaacagc
     2581 tgtagtttgc tttggaagtt aaatgttttg tcaataaaca gggcttgagc acatgcagtg
     2641 ctctcttagg cagtatgtgg tttcagagca tcttggacac atttctcccc tgagaaaata
     2701 ttaatattaa tagaatggga gaagtaagat gcacgcattt ccagaagtat attatttggc
     2761 tgcctaataa attgtgagac aaaagaaaaa aaaaaaaaaa acaggaagag agggacttga
     2821 gggaatttga gggaaatcat aaccaaaagt ttaaaggaaa aaattacacc agacatgata
     2881 agcagaagtt gattttgttc tggtgctatt gccagcatta ggggagaagg cacaaactga
     2941 gtctgatacg tagtgaggca gagacaaaag ggctagggtg gaaaaatttt taaaccatct
     3001 gtgtttacta attccccata ccaaaagaac aaaatgaact ttctcctatt tcatgataaa
     3061 agatagtttc acaatttgga aaaagccccc ccactccaag tttcctaccc ttcccaccca
     3121 cagaaaccag gagacagggg aactatcttc cttgatgtta acatttcaaa gaggtggttc
     3181 tcaggtccta gagaaagata ttcctgagtt gtaaaactgg gaagaggctg gaagaagatt
     3241 tagatctcaa gaaatagaaa gaactacagc aaattttcta aagcaaatat tcttaaggga
     3301 ggtcaagggc ctgaagtcag gaagaaacct atttggtcaa gctgagggga aagttaaggc
     3361 tatcttgtca caagagaaca gaaatatctg acaatatttc atttaaagaa ctgtattagt
     3421 ccattctcac actgctataa ataaatacct gagactgggt aatttataca gaaaagaggt
     3481 ttaattggct cacagttccg caggctgtac aggaagcatg gcaacatcag tttctgggga
     3541 ggcctcaggg agctttaact catggtggaa agcaaagtgg gagcaggcat cttacatggt
     3601 atgagcagga ggaacaaagg aaaggggagg tgctaacact tttaaatgac cagatcttgt
     3661 aagaactcac tcattataca gtaccaaggg gagatggtgc taaacgatta atgaaaactc
     3721 tacccagtaa tccaatcacc acccaccagg ccccacctcc aacaatgggg attacagttt
     3781 gacatgagat ttgggtgagg acacagatcc aaactgtcag gcctctgagc ccaagctaag
     3841 ccatcatatc ccctgtgacc tgcatgtata catccagatg gcctgaagca agtggagaat
     3901 cacaaaagaa gtgaaaatgg cctgttgctg ccttaacaga tgacattact ttgtgaaatt
     3961 ccttcttctg gctcagaagc tcccccactg agcaccttgt gacccccacc cctgcctgcc
     4021 agagaataat cccctttgac tgtaattttc cactacctac ctaaatccta taaaacagcc
     4081 ccacccctat ctccctttgc tgactctctt tttggactca gcctgcctgc acccaggtga
     4141 ttaaaaagct ttattgctca cacaaa
//
