LOCUS       NM_015641               2736 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens testin LIM domain protein (TES), transcript variant 1,
            mRNA.
ACCESSION   NM_015641
VERSION     NM_015641.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Zhang,Y., Wang,G., Zhang,Q., Wang,Q., Luo,J. and Ling,C.
  TITLE     The value of lung function assessment and Testin expression
            detection in clinicopathological features and prognosis of NSCLC
            patients
  JOURNAL   J Cardiothorac Surg 19 (1), 223 (2024)
   PUBMED   38627776
  REMARK    GeneRIF: The value of lung function assessment and Testin
            expression detection in clinicopathological features and prognosis
            of NSCLC patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Sala,S. and Oakes,P.W.
  TITLE     Stress fiber strain recognition by the LIM protein testin is
            cryptic and mediated by RhoA
  JOURNAL   Mol Biol Cell 32 (18), 1758-1771 (2021)
   PUBMED   34038160
  REMARK    GeneRIF: Stress fiber strain recognition by the LIM protein testin
            is cryptic and mediated by RhoA.
REFERENCE   3  (bases 1 to 2736)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2736)
  AUTHORS   Szymanska-Chabowska,A., Juzwiszyn,J., Jankowska-Polanska,B.,
            Tanski,W. and Chabowski,M.
  TITLE     Chitinase 3-Like 1, Nestin, and Testin Proteins as Novel Biomarkers
            of Potential Clinical Use in Colorectal Cancer: A Review
  JOURNAL   Adv Exp Med Biol 1279, 1-8 (2020)
   PUBMED   32170669
  REMARK    GeneRIF: Chitinase 3-Like 1, Nestin, and Testin Proteins as Novel
            Biomarkers of Potential Clinical Use in Colorectal Cancer: A
            Review.
            Review article
REFERENCE   5  (bases 1 to 2736)
  AUTHORS   Block,I., Muller,C., Sdogati,D., Pedersen,H., List,M., Jaskot,A.M.,
            Syse,S.D., Lund Hansen,P., Schmidt,S., Christiansen,H., Casella,C.,
            Bering Olsen,S., Blomstrom,M.M., Riedel,A., Thomassen,M.,
            Kruse,T.A., Karlskov Hansen,S.W., Kioschis,P. and Mollenhauer,J.
  TITLE     CFP suppresses breast cancer cell growth by TES-mediated
            upregulation of the transcription factor DDIT3
  JOURNAL   Oncogene 38 (23), 4560-4573 (2019)
   PUBMED   30755730
  REMARK    GeneRIF: TES-mediated upregulation of the transcription factor
            DDIT3 is involved in CFP suppression of breast cancer cell growth
REFERENCE   6  (bases 1 to 2736)
  AUTHORS   Richards,R.I.
  TITLE     Fragile and unstable chromosomes in cancer: causes and consequences
  JOURNAL   Trends Genet 17 (6), 339-345 (2001)
   PUBMED   11377796
  REMARK    Review article
REFERENCE   7  (bases 1 to 2736)
  AUTHORS   Tobias,E.S., Hurlstone,A.F., MacKenzie,E., McFarlane,R. and
            Black,D.M.
  TITLE     The TES gene at 7q31.1 is methylated in tumours and encodes a novel
            growth-suppressing LIM domain protein
  JOURNAL   Oncogene 20 (22), 2844-2853 (2001)
   PUBMED   11420696
REFERENCE   8  (bases 1 to 2736)
  AUTHORS   Tatarelli,C., Linnenbach,A., Mimori,K. and Croce,C.M.
  TITLE     Characterization of the human TESTIN gene localized in the FRA7G
            region at 7q31.2
  JOURNAL   Genomics 68 (1), 1-12 (2000)
   PUBMED   10950921
REFERENCE   9  (bases 1 to 2736)
  AUTHORS   Bach,I.
  TITLE     The LIM domain: regulation by association
  JOURNAL   Mech Dev 91 (1-2), 5-17 (2000)
   PUBMED   10704826
  REMARK    Review article
REFERENCE   10 (bases 1 to 2736)
  AUTHORS   Grima,J., Zhu,L. and Cheng,C.Y.
  TITLE     Testin is tightly associated with testicular cell membrane upon its
            secretion by sertoli cells whose steady-state mRNA level in the
            testis correlates with the turnover and integrity of
            inter-testicular cell junctions
  JOURNAL   J Biol Chem 272 (10), 6499-6509 (1997)
   PUBMED   9045675
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC341259.1 and AF245356.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015641.3.
            
            Summary: Cancer-associated chromosomal changes often involve
            regions containing fragile sites. This gene maps to a common
            fragile site on chromosome 7q31.2 designated FRA7G. This gene is
            similar to mouse Testin, a testosterone-responsive gene encoding a
            Sertoli cell secretory protein containing three LIM domains. LIM
            domains are double zinc-finger motifs that mediate protein-protein
            interactions between transcription factors, cytoskeletal proteins
            and signaling proteins. This protein is a negative regulator of
            cell growth and may act as a tumor suppressor. This scaffold
            protein may also play a role in cell adhesion, cell spreading and
            in the reorganization of the actin cytoskeleton. Multiple protein
            isoforms are encoded by transcript variants of this gene.[provided
            by RefSeq, Aug 2023].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF245356.1, SRR1163655.315641.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000358204.9/ ENSP00000350937.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                DC341259.1         47-74
            29-2736             AF245356.1         1-2708
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.2"
     gene            1..2736
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /note="testin LIM domain protein"
                     /db_xref="GeneID:26136"
                     /db_xref="HGNC:HGNC:14620"
                     /db_xref="MIM:606085"
     exon            1..196
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..1435
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     testis derived transcript (3 LIM domains)"
                     /codon_start=1
                     /product="testin isoform 1"
                     /protein_id="NP_056456.1"
                     /db_xref="CCDS:CCDS5763.1"
                     /db_xref="GeneID:26136"
                     /db_xref="HGNC:HGNC:14620"
                     /db_xref="MIM:606085"
                     /translation="MDLENKVKKMGLGHEQGFGAPCLKCKEKCEGFELHFWRKICRNC
                     KCGQEEHDVLLSNEEDRKVGKLFEDTKYTTLIAKLKSDGIPMYKRNVMILTNPVAAKK
                     NVSINTVTYEWAPPVQNQALARQYMQMLPKEKQPVAGSEGAQYRKKQLAKQLPAHDQD
                     PSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAA
                     VGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLV
                     DMIYFWKNEKLYCGRHYCDSEKPRCAGCDELIFSNEYTQAENQNWHLKHFCCFDCDSI
                     LAGEIYVMVNDKPVCKPCYVKNHAVVCQGCHNAIDPEVQRVTYNNFSWHASTECFLCS
                     CCSKCLIGQKFMPVEGMVFCSVECKKRMS"
     exon            197..282
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     exon            283..535
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..871
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1087
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1246
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..2736
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2715..2720
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /note="hexamer: AATAAA"
     polyA_site      2736
                     /gene="TES"
                     /gene_synonym="TESS; TESS-2"
                     /note="major polyA site"
ORIGIN      
        1 gctgcggcgg actgggcggc ggaagttcga cggcgccggg cgagtggctg ttgagcggcg
       61 ccgcgggagt tccgcaggtt tcccgtgttc gcagcggagc cggaggccag ctgaacccgg
      121 ccgtgggatc ccggatagga ggaggagggg acccatagga cgcgttaaca tggacctgga
      181 aaacaaagtg aagaagatgg gcttaggtca cgagcaagga tttggagccc cttgtttaaa
      241 atgcaaagaa aaatgtgaag gattcgaact gcacttctgg agaaaaatat gtcgtaactg
      301 caagtgtggc caagaagagc atgatgtcct cttgagcaat gaagaggatc gaaaagtggg
      361 aaaacttttt gaagacacca agtataccac tctgattgca aaactaaagt cagatggaat
      421 tcccatgtat aaacgcaatg ttatgatatt gacgaatcca gttgctgcca agaagaatgt
      481 ctccatcaat acagttacct atgagtgggc tcctcctgtc cagaatcaag cattggccag
      541 gcagtacatg cagatgctac ccaaggaaaa gcagccagta gcaggctcag agggggcaca
      601 gtaccggaag aagcagctgg caaagcagct ccctgcacat gaccaggacc cttcaaagtg
      661 ccatgagttg tctcccagag aggtgaagga gatggagcag tttgtgaaga aatataagag
      721 cgaagctctg ggagtaggag atgtcaaact tccctgtgag atggatgccc aaggccccaa
      781 acaaatgaac attcctggag gggatagaag caccccagca gcagtggggg ccatggagga
      841 caaatctgct gagcacaaaa gaactcaata ttcctgctat tgctgcaaac tgagtatgaa
      901 agaaggtgac ccagccatct atgccgaaag ggctggctat gataaactgt ggcacccagc
      961 ttgttttgtc tgcagcacct gccatgaact cctggttgac atgatttatt tttggaagaa
     1021 tgagaagcta tactgtggca gacattactg tgacagcgag aaaccccgat gtgctggctg
     1081 tgacgagctg atattcagca atgagtatac ccaggcagaa aaccagaatt ggcacctgaa
     1141 acacttctgc tgctttgact gtgatagcat tctagctggg gagatatacg tgatggtcaa
     1201 tgacaagccc gtgtgcaagc cctgctatgt gaagaatcac gctgtggtgt gtcaaggatg
     1261 ccacaatgcc atcgacccag aagtgcagcg ggtgacctat aacaatttca gctggcatgc
     1321 atccacagag tgctttctgt gctcttgctg cagcaaatgc ctcattgggc agaagttcat
     1381 gccagtagaa gggatggttt tctgttcagt ggaatgtaag aagaggatgt cttaggagga
     1441 gggcacccag aagtatcgag ccatagctat ccaaagtggt ctgcatttct actgtaaaat
     1501 gcaatttgaa aaaaataaaa cgcaaaaaaa gaaactgtaa aggaaaccaa gagattttgt
     1561 ttaatttttt tggccatttt ttcttcatca attttttttc ggtctcaact tttaaacttg
     1621 gtttaagcat ttgatttgta aaacagtaaa taattgtatc tttccatagc ttttcaaatg
     1681 tgaaatcatt tttggaagct tggatctcat taaacttcat gtctctattc catttgtgcc
     1741 acacacttaa aagttagtgt actgaatgga aagatgagca ttcctagttc tacacttctt
     1801 ttttccccct catgtgtaaa atgaaaagaa aactaaattt gccctaatac caaggcgcta
     1861 cgtttattgc ctcgtcttat tcactgacct ttgtaatgat acacagtgaa ttctttttga
     1921 caaagagaaa tgcagtgtag tatgcagagc tgctgtttta atgcctatgc atttactctt
     1981 tcctgattta ggcagaggtg gcattttctt tattgcattt ctctattttt ttaatgtacc
     2041 ctaccttcag tattctcttt gtaagttggt gacttgcatc tgtggccttg aatattttat
     2101 tatcacatgt ggcataacag tatccacact ttttagttct ttattttttt ttttttattt
     2161 tgagcaattc tcctgcctca gcctcccaaa tagctgggat tacaggtgca tgccaccaca
     2221 cccagctaat ttttgtattt ttagtagaga caggttttca ccatgttagc caggctggtc
     2281 tcaaactcct gacctcagat gatccgcctg ccttggcctc ccaaagtgct gggattacag
     2341 gtgtgggagc caccatgcct gacccacaca ctttttactt gtatagatga tttttggctt
     2401 ggacataaaa gccaagccac ccatttgctt ttaatccaaa gaacatgtat agtttttgta
     2461 cccagagact atgatttata ttgattgcac ttgcctgcca tgatttagat aagatttttt
     2521 ttgcatggtt tttattcttt cctaatggat cctgttttat aatacttcca agcctgtcca
     2581 tggatatatc aaatgtcttc acttgtatat tttcatggct aggtatttct aatgtttatt
     2641 cttccctgtg tacttctaca catagctatg cactatgaaa attaaatgga atgaatgata
     2701 tgtatattac tcaaaataaa gtttctttca ctttaa
//
