LOCUS       NM_001365324            8440 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens terminal nucleotidyltransferase 4B (TENT4B),
            transcript variant 4, mRNA.
ACCESSION   NM_001365324 XM_005256092
VERSION     NM_001365324.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Li,Y., Misumi,I., Shiota,T., Sun,L., Lenarcic,E.M., Kim,H.,
            Shirasaki,T., Hertel-Wulff,A., Tibbs,T., Mitchell,J.E.,
            McKnight,K.L., Cameron,C.E., Moorman,N.J., McGivern,D.R.,
            Cullen,J.M., Whitmire,J.K. and Lemon,S.M.
  TITLE     The ZCCHC14/TENT4 complex is required for hepatitis A virus RNA
            synthesis
  JOURNAL   Proc Natl Acad Sci U S A 119 (28), e2204511119 (2022)
   PUBMED   35867748
  REMARK    GeneRIF: The ZCCHC14/TENT4 complex is required for hepatitis A
            virus RNA synthesis.
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Shukla,S., Jeong,H.C., Sturgeon,C.M., Parker,R. and Batista,L.F.Z.
  TITLE     Chemical inhibition of PAPD5/7 rescues telomerase function and
            hematopoiesis in dyskeratosis congenita
  JOURNAL   Blood Adv 4 (12), 2717-2722 (2020)
   PUBMED   32559291
  REMARK    GeneRIF: Chemical inhibition of PAPD5/7 rescues telomerase function
            and hematopoiesis in dyskeratosis congenita.
REFERENCE   3  (bases 1 to 8440)
  AUTHORS   Kim,D., Lee,Y.S., Jung,S.J., Yeo,J., Seo,J.J., Lee,Y.Y., Lim,J.,
            Chang,H., Song,J., Yang,J., Kim,J.S., Jung,G., Ahn,K. and Kim,V.N.
  TITLE     Viral hijacking of the TENT4-ZCCHC14 complex protects viral RNAs
            via mixed tailing
  JOURNAL   Nat Struct Mol Biol 27 (6), 581-588 (2020)
   PUBMED   32451488
  REMARK    GeneRIF: Viral hijacking of the TENT4-ZCCHC14 complex protects
            viral RNAs via mixed tailing.
REFERENCE   4  (bases 1 to 8440)
  AUTHORS   Roake,C.M., Chen,L., Chakravarthy,A.L., Ferrell,J.E. Jr.,
            Raffa,G.D. and Artandi,S.E.
  TITLE     Disruption of Telomerase RNA Maturation Kinetics Precipitates
            Disease
  JOURNAL   Mol Cell 74 (4), 688-700 (2019)
   PUBMED   30930056
  REMARK    GeneRIF: We find that mature hTR derives from extended precursors
            but that in PARN-mutant cells hTR maturation kinetically stalls and
            unprocessed precursors are degraded. Loss of poly(A)polymerase
            PAPD5 in PARN-mutant cells accelerates hTR maturation and restores
            hTR processing, indicating that oligoadenylation and deadenylation
            set rates of hTR maturation
REFERENCE   5  (bases 1 to 8440)
  AUTHORS   Lim,J., Kim,D., Lee,Y.S., Ha,M., Lee,M., Yeo,J., Chang,H., Song,J.,
            Ahn,K. and Kim,V.N.
  TITLE     Mixed tailing by TENT4A and TENT4B shields mRNA from rapid
            deadenylation
  JOURNAL   Science 361 (6403), 701-704 (2018)
   PUBMED   30026317
  REMARK    GeneRIF: TENT4A and TENT4B produce a mixed tail that shields mRNA
            from rapid deadenylation.
REFERENCE   6  (bases 1 to 8440)
  AUTHORS   Berndt,H., Harnisch,C., Rammelt,C., Stohr,N., Zirkel,A., Dohm,J.C.,
            Himmelbauer,H., Tavanez,J.P., Huttelmaier,S. and Wahle,E.
  TITLE     Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent
            adenylation and PARN-dependent trimming
  JOURNAL   RNA 18 (5), 958-972 (2012)
   PUBMED   22442037
  REMARK    Erratum:[RNA. 2014 Aug;20(8):1349]
REFERENCE   7  (bases 1 to 8440)
  AUTHORS   Rammelt,C., Bilen,B., Zavolan,M. and Keller,W.
  TITLE     PAPD5, a noncanonical poly(A) polymerase with an unusual
            RNA-binding motif
  JOURNAL   RNA 17 (9), 1737-1746 (2011)
   PUBMED   21788334
  REMARK    GeneRIF: PAPD5 catalyzes the polyadenylation of different types of
            RNA substrates in vitro.
REFERENCE   8  (bases 1 to 8440)
  AUTHORS   Lubas,M., Christensen,M.S., Kristiansen,M.S., Domanski,M.,
            Falkenby,L.G., Lykke-Andersen,S., Andersen,J.S., Dziembowski,A. and
            Jensen,T.H.
  TITLE     Interaction profiling identifies the human nuclear exosome
            targeting complex
  JOURNAL   Mol Cell 43 (4), 624-637 (2011)
   PUBMED   21855801
REFERENCE   9  (bases 1 to 8440)
  AUTHORS   Mullen,T.E. and Marzluff,W.F.
  TITLE     Degradation of histone mRNA requires oligouridylation followed by
            decapping and simultaneous degradation of the mRNA both 5' to 3'
            and 3' to 5'
  JOURNAL   Genes Dev 22 (1), 50-65 (2008)
   PUBMED   18172165
REFERENCE   10 (bases 1 to 8440)
  AUTHORS   Walowsky,C., Fitzhugh,D.J., Castano,I.B., Ju,J.Y., Levin,N.A. and
            Christman,M.F.
  TITLE     The topoisomerase-related function gene TRF4 affects cellular
            sensitivity to the antitumor agent camptothecin
  JOURNAL   J Biol Chem 274 (11), 7302-7308 (1999)
   PUBMED   10066793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC007610.10 and AC007597.3.
            
            On Jul 18, 2019 this sequence version replaced NM_001365324.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FR872509.1, SRR14038197.2337525.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000561678.7/ ENSP00000455837.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-954               AC007610.10        54785-55738         c
            955-1078            AC007597.3         114612-114735       c
            1079-1125           AC007597.3         111791-111837       c
            1126-1246           AC007597.3         109863-109983       c
            1247-1354           AC007597.3         108395-108502       c
            1355-1483           AC007597.3         103624-103752       c
            1484-1697           AC007597.3         102671-102884       c
            1698-1824           AC007597.3         101275-101401       c
            1825-1928           AC007597.3         101064-101167       c
            1929-2116           AC007597.3         100773-100960       c
            2117-2281           AC007597.3         98055-98219         c
            2282-8440           AC007597.3         90748-96906         c
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q12.1"
     gene            1..8440
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /note="terminal nucleotidyltransferase 4B"
                     /db_xref="GeneID:64282"
                     /db_xref="HGNC:HGNC:30758"
                     /db_xref="MIM:605540"
     exon            1..954
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     CDS             317..2458
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /EC_number="2.7.7.19"
                     /note="isoform d is encoded by transcript variant 4;
                     topoisomerase-related function protein 4-2; PAP-associated
                     domain-containing protein 5; TUTase 3; terminal
                     uridylyltransferase 3; non-canonical poly(A) RNA
                     polymerase PAPD5; PAP associated domain containing 5;
                     poly(A) RNA polymerase D5, non-canonical; terminal
                     guanylyltransferase; terminal nucleotidyltransferase 4A"
                     /codon_start=1
                     /product="terminal nucleotidyltransferase 4B isoform d"
                     /protein_id="NP_001352253.1"
                     /db_xref="CCDS:CCDS92158.1"
                     /db_xref="GeneID:64282"
                     /db_xref="HGNC:HGNC:30758"
                     /db_xref="MIM:605540"
                     /translation="MDPRIAWFQPEQLGPSNSLWMQIWETTQGLRNLYFNHHCHSSGG
                     ASGGGGSSSSSSTATGGSGSSTGSPGGAASAPAPAPAGMYRSGERLLGSHALPAEQRD
                     FLPLETTNNNNNHHQPGAWARRAGSSASSPPSASSSPHPSAAVPAADPADSASGSSNK
                     RKRDNKASTYGLNYSLLQPSGGRAAGGGRADGGGVVYSGTPWKRRNYNQGVVGLHEEI
                     SDFYEYMSPRPEEEKMRMEVVNRIESVIKELWPSADVQIFGSFKTGLYLPTSDIDLVV
                     FGKWENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKLTDSFTEVKVDISFNVQNG
                     VRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIGSYSLFLMAVSFLQLHP
                     REDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAKDEVQKNMLDGYRPS
                     MLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYYPNNETESILGRI
                     IRVTDEVATYRDWISKQWGLKNRPEPSCNGNGVTLIVDTQQLDKCNNNLSEENEALGK
                     CRSKTSESLSKHSSNSSSGPVSSSSATQSSSSDVDSDATPCKTPKQLLCRPSTGNRVG
                     SQDVSLESSQAVGKMQSTQTTNTSNSTNKSQHGSARLFRSSSKGFQGTTQTSHGSLMT
                     NKQHQGKSNNQYYHGKKRKHKRDAPLSDLCR"
     exon            955..1078
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1125
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1246
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1354
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1483
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1697
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1824
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1928
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2116
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2281
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..8440
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4937..4942
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /note="hexamer: AGTAAA"
     polyA_site      4955
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /note="major polyA site"
     regulatory      8407..8412
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
                     /note="hexamer: AATAAA"
     polyA_site      8440
                     /gene="TENT4B"
                     /gene_synonym="PAPD5; TRF4-2; TUT3"
ORIGIN      
        1 cttctgtgga gccgccgccg ccgccgccgc catttgcacg gggaccccag tgacaggggc
       61 tcggcggagg ggcggagggg cggagggagg gggggagggc ccgcggagcc cccgagggcg
      121 ggagcgacgc cgccggcgcc ggccgggctc cctgcgcgac cgcgccgccc gcggcgggcc
      181 ccgagcagca gcagcagcag cagcggcagc agcggcagca gcagcagcag ccgaggccgg
      241 gcgtgcgcct gaggcggcgg cggcggcggc cctgcgggcg gccgggaggg gcgggggcag
      301 cggccgccgc cgtttgatgg atccgaggat cgcctggttt cagccagagc agctcggacc
      361 gtccaacagt ctgtggatgc agatctggga gacgacccag gggctgagga acctctactt
      421 caaccaccac tgtcacagca gcggcggcgc gagcggcggc ggcggcagca gcagcagcag
      481 cagcacggcc accggcggga gcggcagcag caccggcagc cccggcggcg cggcctcggc
      541 cccggccccg gccccggccg gcatgtatcg ctccggggag cgcctgctgg gcagccacgc
      601 gctgcccgcg gagcagcggg acttcctgcc cctagagacg accaacaaca acaacaacca
      661 ccaccagccc ggggcctggg cccgccgggc gggctcctcg gcgtcctcgc ctccctcggc
      721 gtcctcgtcc ccgcaccctt cggccgccgt ccccgccgcc gatccagccg attcggcctc
      781 gggcagcagc aacaagagga agcgcgacaa caaggccagc acgtatggac tcaactacag
      841 cctgctgcag cccagcggag ggcgggccgc ggggggcggc cgagcagacg gcggcggggt
      901 cgtgtacagc gggaccccgt ggaaacggag gaactacaac cagggagtcg tgggtctgca
      961 tgaagaaatc agtgattttt atgaatacat gtctccaaga cctgaggagg agaagatgcg
     1021 gatggaggtg gtgaacagga tcgagagtgt aattaaggag ctctggccca gcgctgacgt
     1081 ccagatattt ggaagtttta aaactggact ttatttacct actagtgaca tcgacctagt
     1141 ggtgtttggg aagtgggaga acctacccct ctggactctg gaagaagctc ttcggaaaca
     1201 caaagtcgca gatgaggatt cggtgaaagt tttagacaaa gcaactgtac ctattattaa
     1261 attaacagat tcttttactg aagtgaaagt tgatatcagc tttaatgtac agaatggcgt
     1321 gagagcagct gacctcatca aagattttac caagaaatat cctgtattgc catacttggt
     1381 tttagtattg aaacaattcc tattgcagag ggaccttaat gaagtattta caggtggaat
     1441 tggttcttat agtctctttt taatggcagt cagtttcctt cagttacatc ccagggaaga
     1501 tgcttgcatc cccaatacaa actatggtgt tctcttaata gaattttttg aattatatgg
     1561 acgacacttc aattatttaa agactggcat ccggataaag gatggtggtt catatgtggc
     1621 caaagatgaa gtacagaaaa atatgctaga tggctacagg ccatcaatgc tttatatcga
     1681 agatccttta caaccaggta acgatgttgg aaggagttca tatggggcca tgcaagtgaa
     1741 gcaggccttt gattatgcct acgttgtttt gagtcatgct gtatcaccaa tagcaaagta
     1801 ctatcccaac aatgaaacag aaagcatact aggtagaata attagagtaa cagatgaagt
     1861 tgccacatat agagattgga tatcaaagca gtggggcttg aagaatagac ctgagccttc
     1921 atgcaatgga aatggtgtta ccttgatagt agatactcag cagttagata aatgtaataa
     1981 taatctatct gaagaaaatg aagcccttgg aaaatgtaga agtaaaacct cggaatctct
     2041 tagtaaacac tcttcaaact cttcatcagg tccagtgtcg tcctcttctg ccacacagtc
     2101 cagctctagt gatgtagatt ccgatgcaac accatgcaaa accccgaaac agctgctttg
     2161 ccgtccgtcc actgggaacc gagtagggtc gcaagatgta tccttggagt cctctcaggc
     2221 agttgggaaa atgcaaagca cccaaaccac taacacatcc aacagcacca acaaatctca
     2281 gcatggatca gcaaggctct ttcgttcttc cagcaaaggc ttccaaggta caactcaaac
     2341 aagccatggt tccttgatga caaacaaaca acatcaaggc aaatccaata atcagtatta
     2401 ccatggcaaa aagaggaaac acaagaggga cgcgcccctc tcagacctct gtagatagtc
     2461 agcgctgcgc ggtggactgt cttctctgtg caatgatctc atgctcagga cagttgcgca
     2521 gggactcctg ggagatattc aggagcctca cactgttcag acgttgactt agcaactgcg
     2581 ttttttccca gctcgccaca gaatggatca tgaagactga caactgcaaa aaaaacaaaa
     2641 caaaacaaaa aaaaaagcaa gcaaaaaaga gggaaaaaaa aggctgctta tttgataagt
     2701 catatgctac aacagggtca ttttaagatt taaagcttga atgtaaaata aatatatttc
     2761 tcattggctt tatgcagagt tatagggaat agtattcagt gttggtaggg tgatagaaac
     2821 aaaaaacagt atcagaggat gaggtgggga aggaaaacaa aggtatctga taggaagtcc
     2881 agattccaaa ggggaaagtg atctgtgcat gttttttttt taaatatttt tgcatatatt
     2941 taccatttta ttgtgtgtat atatagaaga ccatatagga gattgatatt tgtaatagtg
     3001 gatttgttaa taatactttt tacataacat tactgtttaa attgtaaaca gattttttct
     3061 caggattagt ttgaaaaata atctaaattg tcatcttaac atccatatat agggaagtga
     3121 ttagttctat tactcaattt gtttttctca gcattgaaat gacttaatag aacccttgtg
     3181 tcctgctgca aaaatttttc ctctctaaag aaaaggttta tggtggcaaa tgatgtttat
     3241 tttattttgt aaaaaaaaaa aaatgtacta tgtacttttg tgtaaacact gaaaaatctc
     3301 tggtcatctc cgagaattaa cttgcaactg ttttctatag tgctgtcgtc ttgggcaatg
     3361 ggcaattaca tgactttgtg tttgcttcct ttgcagtctt ttttttttcc ccccatttct
     3421 tcctaatagg aaaaaaaaaa aaaaaaaggt cacccatgtc tggtctcatt cctgttgcag
     3481 tgaaacttcg agttccacag actttgcatg ctggcttctc taaccctgtg tgctgcgtgt
     3541 gcctgtttct catctcttat tctttttaaa attcatgctt aactactgtg ggagaataac
     3601 tgtaaacagc tttaattaaa tcatacttat aaaaaactat tttcttatat tccactctat
     3661 gcttttggta ttgttgatct ttacaaatta aatggtcttt gataatggat ctattttgta
     3721 ttgccttatt aagaccaaat acttcttgtc atcccattct ttatcctctt ctttcatgga
     3781 attgttatcg ttaattaaaa cttttttaaa cattggcttg tttcaatcat actgtaaatt
     3841 ttggttgtag tcagctttga gtgcaatgag atgtataatt ctgttatcat tacctgttga
     3901 gtttgaaact cagttgggaa tatttaatat aatagaatgt aagtgacatt tctgaaaatg
     3961 ctttctttca gggtgaaagc tcttatgttt agcatcaatg tgtatggctc tgttaaatgc
     4021 agccatttct gagacgagat tcttttatat atatatacat ataaagtact attggctttt
     4081 aggagtttct tttatataca tttatgaaat actgaagacc aatcagacca ttaatggaca
     4141 cttagtgtaa ctttttataa agaaaataat gctaaagtaa gaccaaaact gatgtcatca
     4201 ctgaaattaa caattttcaa tatgttcata ttttaattca caatggaaaa atgtgttcca
     4261 aaactggaaa ctcatagtac tcgtgtaaac tgtggaagat ttcaaatgtg atgttatttt
     4321 gacaatgttt taaattttag agtcacattt tattctgatc agaattttta ttgagatgtt
     4381 gagcttttgt ttttgaaact agtttgtcat aacattgtgc ataatcacag tatttatttt
     4441 ctaggacaat tgtgaatgtg tagacttatg tttactgcta agggaacaat tatttataaa
     4501 ataatattaa atccagtatt agctgcctat ttcagacact taatacttgc agagatctat
     4561 gttacattta ccacactgaa gttttttttg ttgttttttg tttgttttta aagaatcacc
     4621 ctcattgttg aaagtaaatg tactcttagg gtgcgaatat tagtgttcca ataagcatgt
     4681 gattatatta aggtggtggt agcgggaaga taattctgat tccattggga atcttaggtt
     4741 ttcgtaaatt tattgggaaa atagtttttc ctgtactgct gaagtttctt tttggtaaac
     4801 agtatctttc taaaagaaaa aagcatgaag gagaaattga ggtgtgtata catttcctca
     4861 aatgaccagc attgtattcg tgaatactgt gtatcttgca gtgaacagtg tggaagctgt
     4921 tcatttttca atctgaagta aaatactttc aagaactttt agtttgcctg ctcatttgtt
     4981 ttatacattt catctatttg actcctatct tatttctttt ttgagtttta atacttccta
     5041 tattttgtga atatatcaga aatgtgtcat ttatatatta gagtccattc atatccatga
     5101 atcataacct tcctttgcta atacttgttg aatgggattt tacaaattct ccctcactct
     5161 ggtgacattt ctcaggcagt catgtatgtg tacctggcca ttagaaatat taatatttaa
     5221 agactgtttt ttagaggagc tgatgggttg gtgaggtgtc agcacaaaat cttactggtt
     5281 atgttttgat gataaaagta tatccatttt ttccctccag ctttaaggtg actgtgaagg
     5341 tgcctggttt tgaatgtctt tgtttggttt ggagatgtcg cactcagttt tcaaatctag
     5401 cttggatctg taggacctat gttttttaca agtaattgcc ctccagtctt caacagttga
     5461 ttctgtttta tttttatcct gttttgagtg tactttacct ttacttgcat tttgagcctc
     5521 attaatattt aggttatttg atttggctcc agatattcct agatctgcac agggcaaaac
     5581 atgggctata gggtgagcat ttttaattgt ctttttctgc tggaacctta tatctctcca
     5641 tgtgttttct gctccttccc tcccccatga aatggtaagt gtgacttgtg tttgcctgaa
     5701 cctgtggact agtgtttggg gtttctggaa acactagagg gtcagaaaag agtaatgacc
     5761 accgtgacgt gcaggattct cttgctgtga catgttcatt gcaaagccct ctccagtgac
     5821 taggaggtgt agttattaag gttgatctgt tagaaatcac cattattagg tattagtggt
     5881 agatgttgct gatactttta ttggtcatga ctacatctca gttttacttt aatattgatc
     5941 tatagtttga tcagttcctt gaattctaat atgttgattt ctcagtgttt ctgtcactaa
     6001 ccaagaatgt ttctaggcag ttggttgctt cacagtcaaa actaaatggt aaactatcaa
     6061 aaatacattc ccaattttgc tgtgataaat attgaaatgt taaaattaat gaacagaaga
     6121 atttattctt acccatctat tcttgttctc ctagttcatt aaactttcag ttattggaaa
     6181 ggcacattct caaagtattt tatgagcaaa atattctata aatgcgtcta acaaacctaa
     6241 ttgaatataa aagttatatt tagtagttac tgttgatagt aattttcatc agggtcatag
     6301 ttcatctagt aaaatattta gagaatgatg ttaacattcc agcattaaag tgggaacaaa
     6361 gatttatata tgaaattcct taaaagagtt catcttgcct tggtttctga ccctcaagac
     6421 tctagctacc tgccatcttg tcaaaacatt tgtgggtaga ataagtgtta aagatcaaat
     6481 tttaatatgc ttctcgatat ttaacatagc taagaagcca gattttactg tagaagttat
     6541 ttacatgatt tgaaaacttg acctaactgg aagccttttt ctcagtcatc ttgttctaag
     6601 ccatcttgac ttcacaccct tagcgacttt tctttttttt ttggtcaaag ataatgagct
     6661 aaatatatat agacgttgaa tgttgacaaa attattaacc agaaaaattg cttataaagg
     6721 ctgctgatct atttgatacc tagaattaaa tatttgagga cagtttttag ttaataaact
     6781 gctaatgttt attttactgt ctctcaggtt tttggttttt ttaaaaaaaa tgtgtttggc
     6841 ctttacattt tctacttaag tgtgtacttt attgagttta accttgtctg tagcctagta
     6901 gcctgaaaga aaaggagaca gaaccagaga gatggatgta gtgcattccc tttggttatt
     6961 acacatttgt ggtagctcct ggatttactg agagatattt tagctatgtc aataagaaca
     7021 gctaatgatg tggaaatcag gtgttctctt gtgtatttca gtgaacattt ttattagtag
     7081 ttgcatatca tctctagttc cacattttaa cttaacgtct ttgtggcttc accactgagc
     7141 tacctttcac tacaccagct tctgtgtggc ctggtaacat ggaaggtctc tcctaaggac
     7201 agtctggacg tattttgggg gaatgttatt tatcttaaag atgcctagaa acaaaacgca
     7261 tatagtacca gtgagaaact atgaagtaaa caagttgctc aggccgggca tggtggctca
     7321 cgcctgtaat cccagcactt tgggaggccg aagcgggagg atggcttgag gctgggagtt
     7381 tgagaccttc atctcttaaa aaaacaaaca aaaacctgaa tggtgaggtg tggtggaatt
     7441 gggtagggga gggaaaggag gacttggaaa agcattctcc aaagccagca acttggtgaa
     7501 gttcagtact tgcctcttag aggttaggcc atgcctttca aagagagtga aatgatgggt
     7561 tatcagccac attcttggag ttaatatttt tcttcatctt tcagtttggg ttctgtgcta
     7621 ttcatagttc ttccctaaga ccatttcatt attacctttt atatttagtt gcaatttatt
     7681 ataatatgtt gttttgtccc tgaacttaat ctcctaattt taagatcctc tctgattttt
     7741 gcatattgaa acttacagaa gtcactttaa aaaagtcttt tgaaagtcct acaatcctaa
     7801 aataaatcac aagcttgttt gttagacgtg tcaagagtct ccagtcttta ctactaaaaa
     7861 gcagcactgc cttaacacac attgttatgg gtgaaaagtg agggacgacc agtgtagttt
     7921 ctggatataa agtgtgaagg actgttgagt taaacatttt tagtggaata tacatagata
     7981 acgtgtattt agaaactttg gtgaagccag tatttgtttt tagtaacctt tttatgtatt
     8041 tccttctttg attagcattg tcttcagtgt taagaaatgt ggactcctgt gaggtgctgg
     8101 aggtttgaat catcttgaaa actttccaat cttgtctagt taccactgca gagacactaa
     8161 ggaatttacc agaaaaagat atttgataca agtgatttaa gaaatctcaa catttcctga
     8221 ggccgtatca ctgggcaacc agtgatgaaa actatgaatg aattgcacac ctggaagatt
     8281 ttttaagcta atgacagttt cttcaaagat gtcaattatt tgccttggaa attttataaa
     8341 ttgcatttct atgcacatcg gcctctagtg cttaccactc ggtttattat tcataatctg
     8401 caattcaata aaggctttgt gttttcattt atcttcaaaa
//
