LOCUS       NM_014844               8704 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens tectonin beta-propeller repeat containing 2 (TECPR2),
            transcript variant 1, mRNA.
ACCESSION   NM_014844 XM_375105
VERSION     NM_014844.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Khalaf-Nazzal,R., Dweikat,I., Ubeyratna,N., Fasham,J., Alawneh,M.,
            Leslie,J., Maree,M., Gunning,A., Zayed,D.Z., Voutsina,N.,
            McGavin,L., Sawafta,R., Owens,M., Baker,W., Turnpenny,P.,
            Al-Hijawi,F., Baple,E.L., Crosby,A.H. and Rawlins,L.E.
  TITLE     TECPR2-related hereditary sensory and autonomic neuropathy in two
            siblings from Palestine
  JOURNAL   Am J Med Genet A 194 (7), e63579 (2024)
   PUBMED   38436550
  REMARK    GeneRIF: TECPR2-related hereditary sensory and autonomic neuropathy
            in two siblings from Palestine.
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Shalev,I., Somekh,J. and Eran,A.
  TITLE     Multimodal bioinformatic analyses of the neurodegenerative
            disease-associated TECPR2 gene reveal its diverse roles
  JOURNAL   J Med Genet 59 (10), 1002-1009 (2022)
   PUBMED   34933910
  REMARK    GeneRIF: Multimodal bioinformatic analyses of the neurodegenerative
            disease-associated TECPR2 gene reveal its diverse roles.
REFERENCE   3  (bases 1 to 8704)
  AUTHORS   Neuser,S., Brechmann,B., Heimer,G., Brosse,I., Schubert,S.,
            O'Grady,L., Zech,M., Srivastava,S., Sweetser,D.A., Dincer,Y.,
            Mall,V., Winkelmann,J., Behrends,C., Darras,B.T., Graham,R.J.,
            Jayakar,P., Byrne,B., Bar-Aluma,B.E., Haberman,Y., Szeinberg,A.,
            Aldhalaan,H.M., Hashem,M., Al Tenaiji,A., Ismayl,O., Al
            Nuaimi,A.E., Maher,K., Ibrahim,S., Khan,F., Houlden,H.,
            Ramakumaran,V.S., Pagnamenta,A.T., Posey,J.E., Lupski,J.R.,
            Tan,W.H., ElGhazali,G., Herman,I., Munoz,T., Repetto,G.M.,
            Seitz,A., Krumbiegel,M., Poli,M.C., Kini,U., Efthymiou,S.,
            Meiler,J., Maroofian,R., Alkuraya,F.S., Abou Jamra,R., Popp,B.,
            Ben-Zeev,B. and Ebrahimi-Fakhari,D.
  TITLE     Clinical, neuroimaging, and molecular spectrum of TECPR2-associated
            hereditary sensory and autonomic neuropathy with intellectual
            disability
  JOURNAL   Hum Mutat 42 (6), 762-776 (2021)
   PUBMED   33847017
  REMARK    GeneRIF: Clinical, neuroimaging, and molecular spectrum of
            TECPR2-associated hereditary sensory and autonomic neuropathy with
            intellectual disability.
REFERENCE   4  (bases 1 to 8704)
  AUTHORS   Stadel,D., Millarte,V., Tillmann,K.D., Huber,J.,
            Tamin-Yecheskel,B.C., Akutsu,M., Demishtein,A., Ben-Zeev,B.,
            Anikster,Y., Perez,F., Dotsch,V., Elazar,Z., Rogov,V., Farhan,H.
            and Behrends,C.
  TITLE     TECPR2 Cooperates with LC3C to Regulate COPII-Dependent ER Export
  JOURNAL   Mol Cell 60 (1), 89-104 (2015)
   PUBMED   26431026
  REMARK    GeneRIF: Results show that TECPR2 associates with several
            trafficking components as SEC24D and cooperates with LC3C to
            regulate ER exit sites and ER export suggesting that TECPR2
            functions as molecular scaffold linking early secretion pathway and
            autophagy.
REFERENCE   5  (bases 1 to 8704)
  AUTHORS   Kuiper,J.J., Van Setten,J., Ripke,S., Van 'T Slot,R., Mulder,F.,
            Missotten,T., Baarsma,G.S., Francioli,L.C., Pulit,S.L., De
            Kovel,C.G., Ten Dam-Van Loon,N., Den Hollander,A.I., Huis in het
            Veld,P., Hoyng,C.B., Cordero-Coma,M., Martin,J., Llorenc,V.,
            Arya,B., Thomas,D., Bakker,S.C., Ophoff,R.A., Rothova,A., De
            Bakker,P.I., Mutis,T. and Koeleman,B.P.
  TITLE     A genome-wide association study identifies a functional ERAP2
            haplotype associated with birdshot chorioretinopathy
  JOURNAL   Hum Mol Genet 23 (22), 6081-6087 (2014)
   PUBMED   24957906
REFERENCE   6  (bases 1 to 8704)
  AUTHORS   Oz-Levi,D., Gelman,A., Elazar,Z. and Lancet,D.
  TITLE     TECPR2: a new autophagy link for neurodegeneration
  JOURNAL   Autophagy 9 (5), 801-802 (2013)
   PUBMED   23439247
REFERENCE   7  (bases 1 to 8704)
  AUTHORS   Oz-Levi,D., Ben-Zeev,B., Ruzzo,E.K., Hitomi,Y., Gelman,A.,
            Pelak,K., Anikster,Y., Reznik-Wolf,H., Bar-Joseph,I., Olender,T.,
            Alkelai,A., Weiss,M., Ben-Asher,E., Ge,D., Shianna,K.V., Elazar,Z.,
            Goldstein,D.B., Pras,E. and Lancet,D.
  TITLE     Mutation in TECPR2 reveals a role for autophagy in hereditary
            spastic paraparesis
  JOURNAL   Am J Hum Genet 91 (6), 1065-1072 (2012)
   PUBMED   23176824
  REMARK    GeneRIF: The discovered TECPR2 mutation implicates autophagy, a
            central intracellular mechanism, in spastic paraparesis.
REFERENCE   8  (bases 1 to 8704)
  AUTHORS   Behrends,C., Sowa,M.E., Gygi,S.P. and Harper,J.W.
  TITLE     Network organization of the human autophagy system
  JOURNAL   Nature 466 (7302), 68-76 (2010)
   PUBMED   20562859
REFERENCE   9  (bases 1 to 8704)
  AUTHORS   Heimer,G., Neuser,S., Ben-Zeev,B. and Ebrahimi-Fakhari,D.
  TITLE     TECPR2-Related Hereditary Sensory and Autonomic Neuropathy with
            Intellectual Disability
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36137062
REFERENCE   10 (bases 1 to 8704)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP365224.1, AB002327.1,
            AL137229.4, BC136647.1, AL136293.5 and BQ023338.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_014844.4.
            
            Summary: The protein encoded by this gene is a member of the
            tectonin beta-propeller repeat-containing (TECPR) family, and
            contains both TECPR and tryptophan-aspartic acid repeat (WD repeat)
            domains. This gene has been implicated in autophagy, as reduced
            expression levels of this gene have been associated with impaired
            autophagy. Recessive mutations in this gene have been associated
            with a hereditary form of spastic paraparesis (HSP). HSP is
            characterized by progressive spasticity and paralysis of the legs.
            There is also some evidence linking mutations in this gene with
            birdshot chorioretinopathy (BSCR), which results in inflammation of
            the choroid and retina. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB002327.1, BC030791.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000359520.12/ ENSP00000352510.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BP365224.1         32-53
            23-1205             AB002327.1         1-1183
            1206-1206           AL137229.4         101636-101636
            1207-4543           AB002327.1         1185-4521
            4544-6792           BC136647.1         4370-6618
            6793-8218           AL136293.5         79306-80731
            8219-8704           BQ023338.1         22-507              c
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.31"
     gene            1..8704
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /note="tectonin beta-propeller repeat containing 2"
                     /db_xref="GeneID:9895"
                     /db_xref="HGNC:HGNC:19957"
                     /db_xref="MIM:615000"
     exon            1..176
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            177..467
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..4484
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tectonin beta-propeller repeat-containing protein 2"
                     /codon_start=1
                     /product="tectonin beta-propeller repeat-containing
                     protein 2 isoform 1"
                     /protein_id="NP_055659.2"
                     /db_xref="CCDS:CCDS32162.1"
                     /db_xref="GeneID:9895"
                     /db_xref="HGNC:HGNC:19957"
                     /db_xref="MIM:615000"
                     /translation="MASISEPVTFREFCPLYYLLNAIPTKIQKGFRSIVVYLTALDTN
                     GDYIAVGSSIGMLYLYCRHLNQMRKYNFEGKTESITVVKLLSCFDDLVAAGTASGRVA
                     VFQLVSSLPGRNKQLRRFDVTGIHKNSITALAWSPNGMKLFSGDDKGKIVYSSLDLDQ
                     GLCNSQLVLEEPSSIVQLDYSQKVLLVSTLQRSLLFYTEEKSVRQIGTQPRKSTGKFG
                     ACFIPGLCKQSDLTLYASRPGLRLWKADVHGTVQATFILKDAFAGGVKPFELHPRLES
                     PNSGSCSLPERHLGLVSCFFQEGWVLSWNEYSIYLLDTVNQATVAGLEGSGDIVSVSC
                     TENEIFFLKGDRNIIRISSRPEGLTSTVRDGLEMSGCSERVHVQQAEKLPGATVSETR
                     LRGSSMASSVASEPRSRSSSLNSTDSGSGLLPPGLQATPELGKGSQPLSQRFNAISSE
                     DFDQELVVKPIKVKRKKKKKKTEGGSRSTCHSSLESTPCSEFPGDSPQSLNTDLLSMT
                     SSVLGSSVDQLSAESPDQESSFNGEVNGVPQENTDPETFNVLEVSGSMPDSLAEEDDI
                     RTEMPHCHHAHGRELLNGAREDVGGSDVTGLGDEPCPADDGPNSTQLPFQEQDSSPGA
                     HDGEDIQPIGPQSTFCEVPLLNSLTVPSSLSWAPSAEQWLPGTRADEGSPVEPSQEQD
                     ILTSMEASGHLSTNLWHAVTDDDTGQKEIPISERVLGSVGGQLTPVSALAASTHKPWL
                     EQPPRDQTLTSSDEEDIYAHGLPSSSSETSVTELGPSCSQQDLSRLGAEDAGLLKPDQ
                     FAESWMGYSGPGYGILSLVVSEKYIWCLDYKGGLFCSALPGAGLRWQKFEDAVQQVAV
                     SPSGALLWKIEQKSNRAFACGKVTIKGKRHWYEALPQAVFVALSDDTAWIIRTSGDLY
                     LQTGLSVDRPCARAVKVDCPYPLSQITARNNVVWALTEQRALLYREGVSSFCPEGEQW
                     KCDIVSERQALEPVCITLGDQQTLWALDIHGNLWFRTGIISKKPQGDDDHWWQVSITD
                     YVVFDQCSLFQTIIHATHSVATAAQAPVEKVADKLRMAFWSQQLQCQPSLLGVNNSGV
                     WISSGKNEFHVAKGSLIGTYWNHVVPRGTASATKWAFVLASAAPTKEGSFLWLCQSSK
                     DLCSVSAQSAQSRPSTVQLPPEAEMRAYAACQDALWALDSLGQVFIRTLSKSCPTGMH
                     WTRLDLSQLGAVKLTSLACGNQHIWACDSRGGVYFRVGTQPLNPSLMLPAWIMIEPPV
                     QPAGVSLVSVHSSPNDQMLWVLDSRWNVHVRTGITEEMPVGTAWEHVPGLQACQLALS
                     TRTVWARCPNGDLARRYGVTDKNPAGDYWKKIPGSVSCFTVTASDELWAVGPPGYLLQ
                     RLTKTFSHSHGTQKSSQAAMPHPEDLEDEWEVI"
     exon            468..596
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            597..728
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            729..886
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1199
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1332
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1665
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..2642
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2826
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            2827..3000
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3181
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            3182..3323
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3564
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            3565..3654
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            3655..3888
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            3889..4037
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            4038..4179
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            4180..4329
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     exon            4330..8704
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6383..6388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /note="hexamer: AATAAA"
     polyA_site      6404
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /note="major polyA site"
     regulatory      8679..8684
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
                     /note="hexamer: AATAAA"
     polyA_site      8704
                     /gene="TECPR2"
                     /gene_synonym="HSAN9; KIAA0329; SPG49"
ORIGIN      
        1 acttgtggct ctgccgctct agcccccggc ggagccagct gctgctcttc ggtgctggcc
       61 ccggtgccgg ccccgttgcc cagggaacag gctcccggca gcccccgcgg cccggagtcc
      121 atcccgcctc ctccggcccg gcggggccga cgagtccgga ggggctgccg cgggagcccc
      181 caggtttccc tagatgacaa ataaacattc cttttcctgc gtgaagatag tctgtggaaa
      241 ccttggccat ggcatcgata tcagagcctg ttacattcag agagttctgc ccgttgtact
      301 atctcctcaa tgccattccg acaaagatcc agaagggttt ccgctctatc gtggtctatc
      361 tcacggccct cgacaccaac ggggactaca tcgcggtggg cagcagcatc ggcatgctct
      421 atctgtactg ccggcacctc aaccagatga ggaagtacaa ctttgagggg aagacggaat
      481 ctatcactgt ggtgaagctg ctgagctgct ttgatgacct ggtggcagca ggcacagcct
      541 ctggcagggt tgcagttttt caacttgtat cttcattgcc agggagaaat aaacagcttc
      601 ggagatttga tgtcactggt attcacaaaa atagcattac agctctggct tggagcccca
      661 atggaatgaa attgttctct ggagatgaca aaggcaaaat tgtttattct tctctggatc
      721 tagaccaggg gctctgtaac tcccagctgg tgttggagga gccatcttcc attgtgcagc
      781 tggattatag ccagaaagtg ctgctggtct ctactctgca aagaagtctg ctcttttaca
      841 ctgaagaaaa gtctgtaagg caaattggaa cacaaccaag gaaaagtact gggaaatttg
      901 gtgcttgttt tataccagga ctctgtaagc aaagtgatct aaccttgtat gcgtcacggc
      961 ccgggctccg gctatggaag gctgatgtcc acgggactgt tcaagccacg tttatcttaa
     1021 aagatgcttt tgccggggga gtcaagcctt ttgaactgca cccgcgtctg gaatccccca
     1081 acagtggaag ttgcagctta cctgagaggc acctggggct tgtttcatgt ttctttcaag
     1141 aaggctgggt gctgagttgg aatgaatata gtatctatct cctagacaca gtcaaccagg
     1201 ccacagttgc tggtttggaa ggatccggtg atattgtgtc tgtttcgtgc acagaaaatg
     1261 aaatattttt cttgaaagga gataggaaca ttataagaat ttcaagcagg cctgaaggat
     1321 taacatcaac agtgagagat ggtctggaga tgtctggatg ctcagagcgt gtccacgtgc
     1381 agcaagcgga gaagctgcca ggggccacag tttctgagac gaggctcaga ggctcttcca
     1441 tggccagctc cgtggccagc gagccaagga gcaggagcag ctcgctcaac tccaccgaca
     1501 gcggctccgg gctcctgccc cctgggctcc aggccacccc tgagctgggc aagggcagcc
     1561 agcccctgtc acagagattc aacgccatca gctcagagga ctttgaccag gagcttgtcg
     1621 tgaagcctat caaagtgaaa aggaagaaga agaagaagaa gacagaaggt ggaagcagga
     1681 gcacctgtca cagctccctg gaatcgacac cctgctccga atttcctggg gacagtcccc
     1741 agtccttgaa cacagacttg ctgtcgatga cctcaagtgt cctgggcagt agcgtggatc
     1801 agttaagtgc agagtctcca gaccaggaaa gcagcttcaa tggtgaagtg aacggtgtcc
     1861 cacaggaaaa tactgacccc gaaacgttta atgtcctgga ggtgtcagga tcaatgcctg
     1921 attctctggc tgaggaagat gacattagaa ctgaaatgcc acactgtcac catgcacatg
     1981 ggcgggagct gctcaatgga gcgagggaag atgtgggagg cagtgatgtc acgggactcg
     2041 gagatgagcc gtgtcctgca gatgatggac caaatagcac acagttaccc ttccaagaac
     2101 aggacagctc tcctggggcg catgatgggg aagacatcca acccattggc ccccaaagca
     2161 ctttttgtga agtccccctc ctgaactcac tcactgtgcc ttccagcctc agctgggccc
     2221 caagtgctga acagtggctg cctgggacca gagctgatga aggcagcccc gtggagccca
     2281 gccaagagca ggacatccta accagcatgg aggcctctgg ccacctcagc acaaatctct
     2341 ggcatgctgt cactgatgat gacacaggtc agaaagaaat acccatttct gaacgtgtct
     2401 tggggagtgt gggaggacag ctgactccgg tctctgcctt ggcagccagc actcacaagc
     2461 cctggcttga gcagcctcca cgggatcaga cattgacgtc cagcgatgag gaggacatct
     2521 atgcccacgg gcttccttct tcatcctcag agacgagtgt gacagagctc ggacctagtt
     2581 gctcccagca ggacctgagc cggctgggtg cagaggacgc cgggctgctc aagccagatc
     2641 agtttgcaga aagctggatg ggctactcgg gtcccggcta tggcatcctc agcttggtgg
     2701 tctccgagaa gtatatctgg tgcctggact acaaaggcgg cctgttctgc agcgcgttgc
     2761 cgggcgccgg gctgcgctgg cagaagtttg aagatgctgt ccagcaggtg gcagtctcgc
     2821 cctcaggagc ccttctctgg aagattgaac agaaatctaa ccgggctttt gcttgtggga
     2881 aagtcaccat caaggggaag cggcactggt acgaagccct gccccaggca gtgtttgtgg
     2941 ccctgagcga tgacacggcc tggatcatca ggaccagtgg ggacctatac ttgcagacag
     3001 gtctgagcgt ggatcgccct tgtgccagag ccgtaaaggt ggactgtccc tacccgctgt
     3061 cccagatcac agcccggaac aatgtggtgt gggcgctgac agagcagagg gccctcctgt
     3121 accgggaggg cgtgagcagc ttctgtccgg aaggcgagca gtggaagtgt gacattgtca
     3181 gcgaaaggca agctttagaa cccgtctgca taacgctcgg ggatcagcag actctctggg
     3241 ccctggacat ccatgggaac ctgtggttca gaactggcat tatttccaag aagccccaag
     3301 gagatgacga ccattggtgg caagtgagca tcacggacta tgtggtgttt gaccagtgca
     3361 gcttatttca gacgataatc catgccactc actcggtggc cacagcagcc caagcccccg
     3421 tagaaaaggt ggcagataag ctgcgcatgg cgttttggtc ccagcagctt cagtgccagc
     3481 caagccttct cggggtcaat aacagcggtg tctggatctc ctcgggcaag aatgaattcc
     3541 acgtcgctaa gggaagtctc ataggcacct actggaatca tgtggttccc cgtgggacag
     3601 cttctgctac aaaatgggcc tttgtgttgg cttctgcagc tcccacgaag gaaggaagct
     3661 tcctgtggct gtgccagagc agcaaggacc tgtgcagcgt cagcgcccag agcgcacagt
     3721 cgcggccctc cacggtgcag ctgcctcccg aagccgagat gcgcgcctat gccgcctgcc
     3781 aggatgcgct gtgggcgctg gacagcctcg gccaggtgtt catcaggacg ctctccaaga
     3841 gctgccccac gggcatgcac tggaccaggc tggacctctc ccagctagga gctgtaaaat
     3901 tgacaagctt ggcatgtgga aatcagcaca tctgggcctg tgattccagg ggtggagttt
     3961 acttccgtgt agggactcag cctctcaatc ccagtctcat gcttccagcc tggataatga
     4021 ttgagccacc tgtccagccc gccggggtca gcttggtcag cgtccattcc agccccaacg
     4081 accagatgct gtgggtgctt gacagcaggt ggaacgtgca cgtgcggacc gggatcaccg
     4141 aggagatgcc tgtggggacc gcctgggagc atgtgccagg gttgcaggcc tgccagctgg
     4201 cgctgagcac caggaccgtg tgggcccgct gtccaaacgg agacctcgcc cggcggtacg
     4261 gcgtcacaga caagaacccc gccggggact actggaagaa aattcccggc agcgtgtcgt
     4321 gtttcacagt gactgcgtca gatgagctgt gggctgtggg cccgcccggc tacctcctcc
     4381 aacggctgac aaagacgttc agccactcgc acggcaccca gaagagcagc caggccgcca
     4441 tgccccaccc tgaggacctg gaggacgagt gggaggtcat ctgaaggagc cctggccgag
     4501 tcacgcggag gggcccggcg tctgtggcgg gcacaggggc ttcagagtga ctccctggtg
     4561 gacgcgctgc ctcaacactt gtccagacac ctctggccag gttggacccg cacacttact
     4621 ttcatctatg ttggtttctg tctcgttcca gaacccacag cctccacccg tggctggcgt
     4681 gattgctgca gcagtggcgc ctcctagctc aggacagtgg cgactgcccg gctgcatgca
     4741 ctccgattac ccacgtgctg ccgtcctggt ctcatccaca gatagctcca gcttttgttg
     4801 gtgggagtgg tctccggagg cctcccagaa ccaagggtag ccgggcagct ggtttggccc
     4861 agggcctcct tccacattag tagccccagg gccagatgga gccaaaggtc agctctctgc
     4921 agcgcgggat gtgctcggtg atggctttgt cccatcatag gggggtgtcc ccccagagac
     4981 aaagctgcag agcacattcc atgccagacg ctctggccag gaagctgagg ccgggcttga
     5041 gaggagagcg ctggccatgc caggagagaa cccacgcaca tgcacaccac aacacacaac
     5101 acacctcacc tcacaccaca gcacacctca ccacaccaca ccgcactgca ccatacctca
     5161 ccacatctca ccacaccaca gcacacctca ccacacaaca caccacaccc cacaccgcac
     5221 tgcaccgcac cgcaccgcac cgtacctcgc cacatctcac cacaccacac cacaccacac
     5281 ctcactgccc acacacggcg caggctgccc gcctcctgga gagcacactt cagctgaaac
     5341 agtaaagcct gatgggtgca aatggaacct ggatgtgtgc acgtgtgtcc caggtaggga
     5401 cggcacagga gggtgcatgg ggcgtggggg agctgagcaa gggtcgctca cttagaaatg
     5461 tctttggaat ggtgtttaac taatgctgct ggcggacatc ctaaaaccag atgcatcctc
     5521 agaggacgag tctactaatt attgcctttg ttgttgtatt acaaatctgc ataaaatacc
     5581 tcatttcaaa tcaaatctta caaatttaga agagagatat gttttccgaa aacagtggaa
     5641 gccctttgtt ccttcccggg tttgtcctga gcctgcactg tcctcgcctg cagcctcaga
     5701 ggggcaggca tccccgcaca gacttgactg gcagggcggt cacgggacct gcgggctggc
     5761 tccgagtggc agcccatgcc ttctgcgggg tatgggttga cacttgacag gttgaaacca
     5821 gtgcctctat ggacggctgc tgtggcccct tcagacaatg ggcagtgccc accccgccca
     5881 ctggcatctg cgtgtgaggg ctaggccgcc ctgccacaca tcccgccccc tcccggaggc
     5941 agcttcagga caggacacca ggctggctgc tttttttagc ctgcccctgg cccaggccca
     6001 gtccttggtg tcagggagcc cccaggccgc aggtggaggg tgataaaata tgttctctga
     6061 caggacccag ccagccacat aggtggaggt tttccatgtc caaatgaggt caagatgccg
     6121 aaatcccaga tctgacttca cacttccctt ttctagaacc ttttgtaaaa gttggtggca
     6181 gcagaggcag ccccaggccg ggctgcatct ctctgtgtct gttgtgcctt gcccggcgcc
     6241 tcacggatgg caaagctctc ctcacccatg ggactgtagt gcaattaaac ccgcgtctag
     6301 gtgatgcttt taaagttgta gcttcgtgct ttgtacagtt ttctttctgg ttttaatttt
     6361 tagttgtgct ttgagtcagt gcaataaact agactttttc caaacctggc cgagtgtggt
     6421 gcctgagctg tgagaagtgt cctcagccga cactcacgag ggcagtgcaa gggagaacct
     6481 gccagcccag ccccaccaca gggagagtgc gtcagcagac ctgtcctggc ctggctggtg
     6541 ttgaaataca caccggtttg cccaagtggc tgttatgggg gaatggccgc tccagagctg
     6601 gctgtacccc atggtagcct ctgaggagga cgctgtgggg tgaggtccag ggctgcctcc
     6661 tcatggagct gtgtgtgagc aggcgttgga ggggttcgag cccctggttc tgtatcttca
     6721 gccagcaaac gaaaccatat cgcaaaccag agctgctgga aaccagcaca cgcaaaagat
     6781 gacgcccagc acagcagcag gacacaccat gtgccaggaa atgacctcag caagaaacct
     6841 caggccgtgt gaagagcagc aaagctttcc agaaggcata aaaggaaact tgaataaagg
     6901 aagatatgtt ccacattcct ggatggagga ctacacacat tatggaaaga cctcattctt
     6961 tccaaaggaa tttataggtg tagcttgatt tcagttcaaa tccccactgg gattgttttg
     7021 ttggggcagg cagggaggaa taatgttagt acccacaaat gcccatgact gtgcccaatg
     7081 tgcttctcac cagagccctg caaggggaag tctcattagc cccttgagac tcagagaggt
     7141 taggtaactt gctcaaggtc acacagcact gggactggaa gccagctctg tagaactgca
     7201 gacctgtgct gtttgatgcc tcctcactgt cccgtcactc ctgggaagaa gaaacgggtg
     7261 agaactgcta agtgatgaca ggtggagccc cagcaggggc cactctattg aatggcatgg
     7321 cccagaccct cggagggcca ggcaacaggc tagcaaggcc cacagggagg gagcagaatg
     7381 gagagggcat ctcagatgcg cttaaggagc ggcgggggcg gggcatgggg ggtgtcacaa
     7441 ctaatgtggc cactgcttca ttctctgggc aaaataatat taaatggatt aaagaatcta
     7501 aaaccagtgg gaaaactgtc tgatttctgg atggcaaagg ctttctaaac tcaaaagtga
     7561 taggacaagc tacagaggaa aataagtaca tagatttgat ttctccaaaa tacagaaatg
     7621 ctgctgggca cagtgatgtg cagttatagt cccagctact tgggaggcca aggcaggagg
     7681 attacttgag cccaggagtt tgaggctgca atgaactatg atcacaccac tgcactccag
     7741 cctcggcgac agagcaagac cttgtttctc aaaaataaat aaatagaagg aaataaatgt
     7801 aaaaatgctg caaattaaaa acctcaaaga agggaaataa ttgcaacaaa tgggaattga
     7861 tgttgaatat ttaccttaaa aacctataca acaataagag gagtgcgccc ttcagcagca
     7921 tgtatacaaa aattgcaacg atacagagat tagcatggcc cctgcacaag gatgactcgc
     7981 aaattcataa agctttccat aaatatattt attaaaaacc aataggagga gcacttcgag
     8041 tcgagtgtaa gggcccttca caaaagcaga aggaacactg gcccaaaccc ccagcacccc
     8101 ggaagcagag gtgagatggg agcagcttgg gagcccccca ttggcgccgc cctactgggg
     8161 aagccggtcc gtacgtaggc cttgcatctc gccacctcca cttctgtcct caagaacaag
     8221 tcctaggtca agggaaacag cacagcctgt ttataattag gaaacactta aaacagcctg
     8281 gcatttggga acagccatgt taacgtgagc aaattcctct catgaaatag cctgcagcta
     8341 ttaagaagca cgtgtgtgca ggaagcggag gcgcaggacc tcaccgcgcc agcgtgaagt
     8401 tcccgggcat ggtcatgaaa gcgtggttct gtaagaaatc agagggagaa agggagagaa
     8461 gggggaggga gacaacccaa acgttggagg gtatttgttg taaacttcaa acttttggca
     8521 ggtttgaaat ttttcataac tccggggagg agcaagaggg gtgaaaagaa acaagttctc
     8581 tacttgtgat cagcagctgg tcatagtggt tgcctggagt atatgccttt ttgtatcctt
     8641 tgaatttcca gccatgtaaa tgtattattt attccaaaaa taaagcagat ttacatttta
     8701 aaaa
//
