LOCUS       NM_153046               4788 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tudor domain containing 9 (TDRD9), mRNA.
ACCESSION   NM_153046 XM_058619
VERSION     NM_153046.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Mo,H.Y., Choi,E.J., Yoo,N.J. and Lee,S.H.
  TITLE     Mutational alterations of TDRD 1, 4 and 9 genes in colorectal
            cancers
  JOURNAL   Pathol Oncol Res 26 (3), 2007-2008 (2020)
   PUBMED   32036563
  REMARK    GeneRIF: Mutational alterations of TDRD 1, 4 and 9 genes in
            colorectal cancers.
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Babakhanzadeh,E., Khodadadian,A., Rostami,S., Alipourfard,I.,
            Aghaei,M., Nazari,M., Hosseinnia,M., Mehrjardi,M.Y.V., Jamshidi,Y.
            and Ghasemi,N.
  TITLE     Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in
            azoospermia
  JOURNAL   BMC Med Genet 21 (1), 33 (2020)
   PUBMED   32059713
  REMARK    GeneRIF: Here we evaluate gene and protein expression levels of
            four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular
            biopsy samples obtained from men with obstructive azoospermia (OA,
            n = 29), as controls, and various types of non-obstructive
            azoospermia containing hypospermatogenesis (HP, 28), maturation
            arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32)
            as cases.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4788)
  AUTHORS   Arafat,M., Har-Vardi,I., Harlev,A., Levitas,E., Zeadna,A.,
            Abofoul-Azab,M., Dyomin,V., Sheffield,V.C., Lunenfeld,E.,
            Huleihel,M. and Parvari,R.
  TITLE     Mutation in TDRD9 causes non-obstructive azoospermia in infertile
            men
  JOURNAL   J Med Genet 54 (9), 633-639 (2017)
   PUBMED   28536242
  REMARK    GeneRIF: first report of a recessive deleterious mutation in TDRD9
            in humans; study demonstrates that non-obstructive azoospermia can
            be caused by a mutation in TDRD9
REFERENCE   4  (bases 1 to 4788)
  AUTHORS   Jiang,Y. and Zhang,H.
  TITLE     Propensity score-based nonparametric test revealing genetic
            variants underlying bipolar disorder
  JOURNAL   Genet Epidemiol 35 (2), 125-132 (2011)
   PUBMED   21254220
REFERENCE   5  (bases 1 to 4788)
  AUTHORS   Feng,T. and Zhu,X.
  TITLE     Genome-wide searching of rare genetic variants in WTCCC data
  JOURNAL   Hum Genet 128 (3), 269-280 (2010)
   PUBMED   20549515
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   6  (bases 1 to 4788)
  AUTHORS   Secolin,R., Banzato,C.E., Oliveira,M.C., Bittar,M.F., Santos,M.L.,
            Dalgalarrondo,P. and Lopes-Cendes,I.
  TITLE     Family-based association study for bipolar affective disorder
  JOURNAL   Psychiatr Genet 20 (3), 126-129 (2010)
   PUBMED   20414141
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 4788)
  AUTHORS   Shoji,M., Tanaka,T., Hosokawa,M., Reuter,M., Stark,A., Kato,Y.,
            Kondoh,G., Okawa,K., Chujo,T., Suzuki,T., Hata,K., Martin,S.L.,
            Noce,T., Kuramochi-Miyagawa,S., Nakano,T., Sasaki,H., Pillai,R.S.,
            Nakatsuji,N. and Chuma,S.
  TITLE     The TDRD9-MIWI2 complex is essential for piRNA-mediated
            retrotransposon silencing in the mouse male germline
  JOURNAL   Dev Cell 17 (6), 775-787 (2009)
   PUBMED   20059948
REFERENCE   8  (bases 1 to 4788)
  AUTHORS   Ollila,H.M., Soronen,P., Silander,K., Palo,O.M., Kieseppa,T.,
            Kaunisto,M.A., Lonnqvist,J., Peltonen,L., Partonen,T. and Paunio,T.
  TITLE     Findings from bipolar disorder genome-wide association studies
            replicate in a Finnish bipolar family-cohort
  JOURNAL   Mol Psychiatry 14 (4), 351-353 (2009)
   PUBMED   19308021
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 4788)
  CONSRTM   Wellcome Trust Case Control Consortium
  TITLE     Genome-wide association study of 14,000 cases of seven common
            diseases and 3,000 shared controls
  JOURNAL   Nature 447 (7145), 661-678 (2007)
   PUBMED   17554300
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL132712.4 and AL136001.6.
            
            On Nov 23, 2018 this sequence version replaced NM_153046.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.193321.1,
                                           SRR1803615.197502.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409874.9/ ENSP00000387303.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               AL132712.4         17753-18021         c
            270-376             AL136001.6         21666-21772
            377-474             AL136001.6         29081-29178
            475-696             AL136001.6         31335-31556
            697-819             AL136001.6         32711-32833
            820-900             AL136001.6         36543-36623
            901-1065            AL136001.6         41391-41555
            1066-1169           AL136001.6         52219-52322
            1170-1234           AL136001.6         57162-57226
            1235-1289           AL136001.6         60334-60388
            1290-1374           AL136001.6         60521-60605
            1375-1432           AL136001.6         61752-61809
            1433-1537           AL136001.6         64626-64730
            1538-1635           AL136001.6         70240-70337
            1636-1767           AL136001.6         71276-71407
            1768-1933           AL136001.6         72391-72556
            1934-1997           AL136001.6         72648-72711
            1998-2061           AL136001.6         72784-72847
            2062-2106           AL136001.6         73162-73206
            2107-2160           AL136001.6         74415-74468
            2161-2277           AL136001.6         80727-80843
            2278-2385           AL136001.6         81983-82090
            2386-2486           AL136001.6         84094-84194
            2487-2660           AL136001.6         88159-88332
            2661-2772           AL136001.6         90571-90682
            2773-2985           AL136001.6         91566-91778
            2986-3075           AL136001.6         92049-92138
            3076-3336           AL136001.6         92681-92941
            3337-3492           AL136001.6         97110-97265
            3493-3563           AL136001.6         98019-98089
            3564-3673           AL136001.6         99962-100071
            3674-3770           AL136001.6         100962-101058
            3771-3909           AL136001.6         106198-106336
            3910-4028           AL136001.6         108071-108189
            4029-4101           AL136001.6         115610-115682
            4102-4788           AL136001.6         117983-118669
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.33"
     gene            1..4788
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /note="tudor domain containing 9"
                     /db_xref="GeneID:122402"
                     /db_xref="HGNC:HGNC:20122"
                     /db_xref="MIM:617963"
     exon            1..269
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..4203
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /EC_number="3.6.4.13"
                     /note="putative ATP-dependent RNA helicase TDRD9;
                     hypoxia-inducible HIG-1; tudor domain-containing protein
                     9"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase TDRD9"
                     /protein_id="NP_694591.2"
                     /db_xref="CCDS:CCDS9987.2"
                     /db_xref="GeneID:122402"
                     /db_xref="HGNC:HGNC:20122"
                     /db_xref="MIM:617963"
                     /translation="MLRKLTIEQINDWFTIGKTVTNVELLGAPPAFPAGAAREEVQRQ
                     DVAPGAGPAAQAPALAQAPARPAAAFERSLSQRSSEVEYINKYRQLEAQELDVCRSVQ
                     PTSGPGPRPSLAKLSSVTCIPGTTYKYPDLPISRYKEEVVSLIESNSVVIIHGATGSG
                     KSTQLPQYILDHYVQRSAYCSIVVTQPRKIGASSIARWISKERAWTLGGVVGYQVGLE
                     KIATEDTRLIYMTTGVLLQKIVSAKSLMEFTHIIIDEVHERTEEMDFLLLVVRKLLRT
                     NSRFVKVVLMSATISCKEFADYFAVPVQNKMNPAYIFEVEGKPHSVEEYYLNDLEHIH
                     HSKLSPHLLEEPVITKDIYEVAVSLIQMFDDLDMKESGNKAWSGAQFVLERSSVLVFL
                     PGLGEINYMHELLTSLVHKRLQVYPLHSSVALEEQNNVFLSPVPGYRKIILSTNIAES
                     SVTVPDVKYVIDFCLTRTLVCDEDTNYQSLRLSWASKTSCNQRKGRAGRVSRGYCYRL
                     VHKDFWDNSIPDHVVPEMLRCPLGSTILKVKLLDMGEPRALLATALSPPGLSDIERTI
                     LLLKEVGALAVSGQREDENPHDGELTFLGRVLAQLPVNQQLGKLIVLGHVFGCLDECL
                     IIAAALSLKNFFAMPFRQHLDGYRNKVNFSGSSKSDCIALVEAFKTWKACRQTGELRY
                     PKDELNWGRLNYIQIKRIREVAELYEELKTRISQFNMHVDSRRPVMDQEYIYKQRFIL
                     QVVLAGAFYPNYFTFGQPDEEMAVRELAGKDPKTTVVLKHIPPYGFLYYKQLQSLFRQ
                     CGQVKSIVFDGAKAFVEFSRNPTERFKTLPAVYMAIKMSQLKVSLELSVHSAEEIEGK
                     VQGMNVSKLRNTRVNVDFQKQTVDPMQVSFNTSDRSQTVTDLLLTIDVTEVVEVGHFW
                     GYRIDENNSEILKKLTAEINQLTLVPLPTHPHPDLVCLAPFADFDKQRYFRAQVLYVS
                     GNSAEVFFVDYGNKSHVDLHLLMEIPCQFLELPFQALEFKICKMRPSAKSLVCGKHWS
                     DGASQWFASLVSGCTLLVKVFSVVHSVLHVDVYQYSGVQDAINIRDVLIQQGYAELTE
                     ESYESKQSHEVLKGLFSKSVENMTDGSVPFPMKDDEKYLIRILLESFSTNKLGTPNCK
                     AELHGPFNPYELKCHSLTRISKFRCVWIEKESINSVIISDAPEDLHQRMLVAASLSIN
                     ATGSTMLLRETSLMPHIPGLPALLSMLFAPVIELRIDQNGKYYTGVLCGLGWNPATGA
                     SILPEHDMELAFDVQFSVEDVVEVNILRAAINKLVCDGPNGCKCLGPERVAQLQDIAR
                     QKLLGLFCQSKPREKIVPKWHEKPYEWNQVDPKLVMEQADRESSRGKNTFLYQLHKLV
                     VLGT"
     exon            270..376
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            377..474
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            475..696
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            697..819
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            820..900
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1065
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1169
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1234
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1289
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1374
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1432
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1537
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1635
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1767
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1933
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..1997
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2061
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2106
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2160
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2277
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2385
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2486
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..2660
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2772
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2985
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3075
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3336
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3337..3492
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3493..3563
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3564..3673
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3674..3770
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3771..3909
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            3910..4028
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4101
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     exon            4102..4788
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4770..4775
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /note="hexamer: AATAAA"
     polyA_site      4788
                     /gene="TDRD9"
                     /gene_synonym="C14orf75; HIG-1; HLS; NET54; SPGF30; SPNE"
                     /note="major polyA site"
ORIGIN      
        1 gttcccgccg cggagacccg gcagttgggg gatgccgacg cctgggcctt gaggatgctg
       61 cggaagctca ccatcgagca gatcaacgac tggttcacca tcggcaagac ggtgaccaat
      121 gtggagctgc tgggcgcgcc gcccgccttc ccggcagggg cggccaggga ggaggtgcag
      181 cgccaggacg tggcccccgg cgctggtccc gcggcccagg ctccggctct ggcccaagct
      241 ccggcccggc cggccgctgc gttcgaaagg tcactcagcc aaaggagctc agaagtagag
      301 tatattaaca aatacagaca gctcgaagca caagagcttg atgtgtgtcg cagtgtccaa
      361 ccaaccagtg ggccaggtcc aaggccatct ttggctaaat taagcagtgt gacatgcatc
      421 ccagggacaa cttataaata tcctgatttg cctataagtc gatacaagga agaggttgtg
      481 tctttgatag aaagtaattc cgtggtgatt atccatgggg ccacgggaag cggtaaaagc
      541 actcagctcc cgcagtatat cttggaccac tacgttcagc gctccgccta ctgcagcatt
      601 gtggtcaccc agccccggaa gataggggca agcagcatcg ccaggtggat cagtaaagag
      661 cgtgcctgga ccctgggagg tgtggtgggc taccaggtag ggctagagaa aatagcaaca
      721 gaggacacca ggctaattta tatgacaact ggagtcctgc ttcagaaaat agttagtgcc
      781 aagagtttga tggaattcac acatatcatc attgatgaag tacacgaacg aacagaagaa
      841 atggatttcc tgctattggt agtccgcaaa ctcttaagaa caaattcacg ttttgtgaag
      901 gtggtcctga tgtcggctac catcagctgt aaagagtttg cagactactt tgctgttcct
      961 gttcaaaaca agatgaatcc tgcatatatt tttgaagtgg aaggcaagcc ccattcagtt
     1021 gaagagtatt atcttaatga tttggagcac attcatcata gcaagctctc tcctcatctc
     1081 ctggaggaac cggtgataac taaggatata tatgaagttg ctgtctctct cattcagatg
     1141 tttgatgact tggatatgaa ggagagtggg aacaaggctt ggtcgggggc ccagtttgtg
     1201 ttggagcgaa gcagtgtgtt ggtgtttttg ccaggtctgg gtgaaataaa ttatatgcat
     1261 gaacttctca caagcctggt tcataaaagg ttgcaggtct atccactcca ttcaagtgtg
     1321 gctttagaag aacagaataa tgtcttttta agtccagtcc ctgggtacag aaagattatt
     1381 ctgtccacca atattgcaga gagttctgtc acagttccag atgtcaaata tgttatagat
     1441 ttttgtttga ctagaacttt ggtctgtgat gaagatacaa attatcagag tctgcgattg
     1501 agttgggctt ctaaaaccag ctgtaatcag agaaaaggcc gtgctggacg agtgtctaga
     1561 gggtactgtt accggctggt acacaaggat ttctgggaca actccatccc tgatcatgtt
     1621 gttcctgaga tgttgcgttg tccattagga agcacgatct tgaaagtgaa attacttgac
     1681 atgggtgagc cgagagctct gctggccact gccctttccc cgcctggtct gagtgacatt
     1741 gagcgcacca tccttctact aaaggaggtt ggagcacttg cagtgagtgg gcagagagaa
     1801 gatgaaaacc cccatgatgg tgaattgacc ttcttaggaa gagttttagc ccaacttcct
     1861 gtaaatcagc aacttggtaa actcatagtc cttggacatg tatttggatg tctagatgaa
     1921 tgtcttatta tagcggcagc tctttctttg aagaattttt ttgcaatgcc tttccggcag
     1981 catctcgatg gatataggaa caaagtgaat ttctctggca gtagcaagag tgactgtatt
     2041 gcacttgttg aggcatttaa aacatggaag gcttgcagac agacagggga gctgcggtac
     2101 ccgaaggatg aacttaattg gggacggtta aattacattc aaatcaagag aattagagag
     2161 gtggctgaat tatatgaaga attgaagact agaatctcac agttcaacat gcatgttgat
     2221 tctcggcgac ctgtcatgga ccaagagtat atatataagc agcgattcat cctacaggtt
     2281 gtattggcag gtgctttcta tccaaattac tttacttttg gacagccgga tgaggagatg
     2341 gcggtgaggg agctggctgg caaggacccc aagacaactg tcgtgttgaa acacattcct
     2401 ccctatggat ttctttacta taaacaacta cagtctctct ttagacagtg tggtcaagtc
     2461 aaatccattg tatttgatgg tgcaaaagcc tttgtggaat tctcacgaaa tccaacagag
     2521 agatttaaaa cccttcctgc agtatatatg gcaattaaga tgtctcaact aaaagtttca
     2581 cttgaactca gcgttcattc tgcagaggaa attgaaggga aggtgcaagg catgaacgtc
     2641 tcaaagctca ggaacacaag ggtgaatgtg gacttccaga agcagacggt agatcctatg
     2701 caagtctcct ttaacacatc agacaggtcc cagacagtta cagatctcct tctaactatt
     2761 gatgtcacag aggtggttga agtgggacac ttttggggat acaggattga tgaaaacaac
     2821 tcagagattc tgaaaaagct tactgctgaa atcaaccaac tgacgctggt gcccttgccc
     2881 actcacccac atccagactt ggtctgtctg gcaccttttg ctgattttga taaacaacgc
     2941 tactttagag ctcaagtcct ttatgtttct ggaaattctg ctgaggtatt ctttgtagat
     3001 tatggcaata agtctcatgt agatctacat cttttgatgg agattccctg tcaatttctt
     3061 gaacttcctt tccaggcttt ggaatttaag atttgcaaaa tgagaccatc agcaaagtct
     3121 cttgtttgtg gcaagcactg gagtgacggg gccagccagt ggttcgcctc tctggtgagc
     3181 ggctgcaccc tccttgtgaa ggtcttctct gtggtgcaca gcgtcctgca cgtggatgtg
     3241 taccagtact caggggtcca ggatgccatc aacataagag acgtcctcat ccagcagggc
     3301 tatgccgagc tcacggagga gtcctacgag tccaagcaaa gccatgaagt tctcaagggc
     3361 ctcttttcca agtcagtaga aaacatgaca gatggctctg tgccctttcc catgaaagac
     3421 gacgagaaat atctcatccg gattttgtta gagagctttt ctaccaataa actgggtact
     3481 ccaaactgta aggcagaact tcacgggcct tttaaccctt atgaactaaa gtgccatagt
     3541 ttgaccagaa tatccaaatt caggtgtgtt tggattgaga aggagagcat caactctgtc
     3601 attatcagtg acgcccctga agaccttcac cagagaatgc tggttgcagc ttccctttcc
     3661 atcaatgcga ctggatctac gatgctgctg agagaaacct ctctgatgcc tcatatccct
     3721 ggcctcccgg ctctcctcag catgttattc gcaccggtga tagagttaag gattgatcag
     3781 aatggcaagt actatactgg agtcctttgt ggtttggggt ggaatccagc tacaggggct
     3841 tccatactgc ccgagcacga catggagctt gcgtttgacg ttcaattcag cgtggaggat
     3901 gtcgtcgagg ttaatattct cagggctgct attaacaagc tagtctgtga tggaccaaat
     3961 ggatgcaagt gtcttgggcc agagagagtt gcgcagcttc aagacattgc ccgtcagaag
     4021 cttttaggtt tgttctgtca gtcaaaacca agggagaaga ttgttcccaa gtggcatgaa
     4081 aagccctacg agtggaatca ggttgatcca aagctggtca tggagcaggc cgaccgtgag
     4141 agcagcagag ggaagaacac ctttctctac cagctccaca aactggttgt gctcggcacc
     4201 tgagcatgtc cacaggtggc ctccagcaca cccctcagga agctgtggag gctggattcc
     4261 aggctccctc cgcagactga ctttcctctg tgtctgggtg ttacagtctg tgcccactgc
     4321 atcctaaagg ccttttcttt cttcttttct ctttgggtga tagtcagaga gtggtgtttt
     4381 tgttcaggtg ggaaggattg gaaactctag tcttttctag aaacagaaaa tcactgtatt
     4441 aaatattttg gaaagattgt tctgaaagaa gtctgtttgg ataaagagct gtattttgct
     4501 ttaaatttat taaggtaaat ataagtagtt aatcttagat gtaaggttcc agaatgtgct
     4561 tacatattct gttctgttac agtgatttaa accagtagta taggaaaaaa cttaaaaaaa
     4621 caaaaaaacc atgtagtatt ttctgatttt tttttccatg agggaaaata tctaattttt
     4681 ataagactaa gttgagttat acttcttggt tcacattttg gaaatcagag attacagatt
     4741 acatggccat agcttatctg tgttaaaaca ataaaagcat taaatgaa
//
