LOCUS       NM_001010870            8967 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens tudor domain containing 6 (TDRD6), transcript variant
            1, mRNA.
ACCESSION   NM_001010870 XM_166443
VERSION     NM_001010870.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Guo,R., Wu,H., Zhu,X., Wang,G., Hu,K., Li,K., Geng,H., Xu,C.,
            Zu,C., Gao,Y., Tang,D., Cao,Y. and He,X.
  TITLE     Bi-allelic variants in chromatoid body protein TDRD6 cause
            spermiogenesis defects and severe oligoasthenoteratozoospermia in
            humans
  JOURNAL   J Med Genet 61 (6), 553-565 (2024)
   PUBMED   38341271
  REMARK    GeneRIF: Bi-allelic variants in chromatoid body protein TDRD6 cause
            spermiogenesis defects and severe oligoasthenoteratozoospermia in
            humans.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Sha,Y.W., Wang,X., Su,Z.Y., Wang,C., Ji,Z.Y., Mei,L.B., Zhang,L.,
            Deng,B.B., Huang,X.J., Yan,W., Chen,J., Li,P., Cui,Y.Q., Qu,Q.L.,
            Yin,C. and He,X.M.
  TITLE     TDRD6 is associated with oligoasthenoteratozoospermia by sequencing
            the patient from a consanguineous family
  JOURNAL   Gene 659, 84-88 (2018)
   PUBMED   29551503
  REMARK    GeneRIF: Whole-exome sequencing results in a patient with
            oligoasthenoteratozoospermia (OAT) from a consanguineous family
            identified a rare homozygous variant in TDRD6 which co-segregated
            with the OAT phenotype.
REFERENCE   3  (bases 1 to 8967)
  AUTHORS   Fanourgakis,G., Lesche,M., Akpinar,M., Dahl,A. and Jessberger,R.
  TITLE     Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered
            Nonsense Mediated mRNA Decay
  JOURNAL   PLoS Genet 12 (5), e1005857 (2016)
   PUBMED   27149095
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8967)
  AUTHORS   Vasileva,A., Tiedau,D., Firooznia,A., Muller-Reichert,T. and
            Jessberger,R.
  TITLE     Tdrd6 is required for spermiogenesis, chromatoid body architecture,
            and regulation of miRNA expression
  JOURNAL   Curr Biol 19 (8), 630-639 (2009)
   PUBMED   19345099
REFERENCE   5  (bases 1 to 8967)
  AUTHORS   Bensing,S., Fetissov,S.O., Mulder,J., Perheentupa,J.,
            Gustafsson,J., Husebye,E.S., Oscarson,M., Ekwall,O., Crock,P.A.,
            Hokfelt,T., Hulting,A.L. and Kampe,O.
  TITLE     Pituitary autoantibodies in autoimmune polyendocrine syndrome type
            1
  JOURNAL   Proc Natl Acad Sci U S A 104 (3), 949-954 (2007)
   PUBMED   17215373
REFERENCE   6  (bases 1 to 8967)
  AUTHORS   Hosokawa,M., Shoji,M., Kitamura,K., Tanaka,T., Noce,T., Chuma,S.
            and Nakatsuji,N.
  TITLE     Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain
            composition, intracellular localization, and function in male germ
            cells in mice
  JOURNAL   Dev Biol 301 (1), 38-52 (2007)
   PUBMED   17141210
REFERENCE   7  (bases 1 to 8967)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   8  (bases 1 to 8967)
  AUTHORS   Scanlan,M.J., Chen,Y.T., Williamson,B., Gure,A.O., Stockert,E.,
            Gordan,J.D., Tureci,O., Sahin,U., Pfreundschuh,M. and Old,L.J.
  TITLE     Characterization of human colon cancer antigens recognized by
            autologous antibodies
  JOURNAL   Int J Cancer 76 (5), 652-658 (1998)
   PUBMED   9610721
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK131455.1 and AL591242.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001010870.2.
            
            Summary: This gene encodes a tudor domain-containing protein and
            component of the chromatoid body, a type of ribonucleoprotein
            granule present in male germ cells. Studies in rodents have
            demonstrated a role for the encoded protein in spermiogenesis and
            the nonsense mediated decay (NMD) pathway. This protein is a major
            autoantigen in human patients with autoimmune polyendocrine
            syndrome type 1 (APS1). [provided by RefSeq, Oct 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR936792.1, SRR1803617.96620.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145544
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316081.11/ ENSP00000346065.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3727              AK131455.1         11-3737
            3728-6290           AL591242.4         52275-54837
            6291-6415           AL591242.4         56484-56608
            6416-6505           AL591242.4         58661-58750
            6506-8967           AL591242.4         62521-64982
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.3"
     gene            1..8967
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="tudor domain containing 6"
                     /db_xref="GeneID:221400"
                     /db_xref="HGNC:HGNC:21339"
                     /db_xref="MIM:611200"
     exon            1..6290
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..6535
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cancer/testis antigen 41.2; tudor domain-containing
                     protein 6; antigen NY-CO-45; spermatogenesis associated
                     36"
                     /codon_start=1
                     /product="tudor domain-containing protein 6 isoform 1"
                     /protein_id="NP_001010870.1"
                     /db_xref="CCDS:CCDS34470.1"
                     /db_xref="GeneID:221400"
                     /db_xref="HGNC:HGNC:21339"
                     /db_xref="MIM:611200"
                     /translation="MCSTPGMPAPGASLALRVSFVDVHPDVIPVQLWGLVGERRGEYL
                     RLSREIQEAAATRGQWALGSASASPGELCLVQVGLLWHRCRVVSRQAQESRVFLLDEG
                     RTITAGAGSLAPGRREFFNLPSEVLGCVLAGLVPAGCGAGSGEPPQHWPADAVDFLSN
                     LQGKEVHGCVLDVLLLHRLVLLEVPDVFQQMRELGLARRVPDSLFRSLLERYLTAATA
                     SVGSGVPVLSRVPLKQKQPGLDYFYPQLQLGVTEAVVITQVCHPHRIHCQLRSVSQEI
                     HRLSESMAQVYRGSTGTGDENSTSATWEEREESPDKPGSPCASCGLDGHWYRALLLET
                     FRPQRCAQVLHVDYGRKELVSCSSLRYLLPEYFRMPVVTYPCALYGLWDGGRGWSRSQ
                     VGDLKTLILGKAVNAKIEFYCSFEHVYYVSLYGEDGINLNRVFGVQSCCLADRVLQSQ
                     ATEEEEPETSQSQSPAEEVDEEISLPALRSIRLKMNAFYDAQVEFVKNPSEFWIRLRK
                     HNVTFSKLMRRMCGFYSSASKLDGVVLKPEPDDLCCVKWKENGYYRAIVTKLDDKSVD
                     VFLVDRGNSENVDWYDVRMLLPQFRQLPILAVKCTLADIWPLGKTWSQEAVSFFKKTV
                     LHKELVIHILDKQDHQYVIEILDESRTGEENISKVIAQAGYAKYQEFETKENILVNAH
                     SPGHVSNHFTTESNKIPFAKTGEGEQKAKRENKTTSVSKALSDTTVVTNGSTELVVQE
                     KVKRASVYFPLMQNCLEIKPGSSSKGELEVGSTVEVRVSYVENPGYFWCQLTRNIQGL
                     KTLMSDIQYYCKNTAAPHQRNTLACLAKRTVNRQWSRALISGIQSVEHVNVTFVDYGD
                     REMVSVKNIYSISEEFLKVKAQAFRCSLYNLIQPVGQNPFVWDVKAIQAFNEFIDNAW
                     QKNLELKCTIFALASINEELFNIVDLLTPFQSACHFLVEKRLARPVKLQKPLESSVQL
                     HSYFYSTHDMKIGSEELVYITHIDDPWTFYCQLARNANILEQLSCSITQLSKVLLNLK
                     TSPLNPGTLCLAKYTDGNWYRGIVIEKEPKKVFFVDFGNIYVVTSDDLLPIPSDAYDV
                     LLLPMQAVRCSLSDIPDHIPEEVVVWFQETILDKSLKALVVAKDPDGTLIIELYGDNI
                     QISASINKKLGLLSYKDRIRKKESEVLCSTTETLEEKNENMKLPCTEYLSKSVGYKLP
                     NKEILEESYKPQINSSYKELKLLQSLTKTNLVTQYQDSVGNKNSQVFPLTTEKKEEIS
                     AETPLKTARVEATLSERKIGDSCDKDLPLKFCEFPQKTIMPGFKTTVYVSHINDLSDF
                     YVQLIEDEAEISHLSERLNSVKTRPEYYVGPPLQRGDMICAVFPEDNLWYRAVIKEQQ
                     PNDLLSVQFIDYGNVSVVHTNKIGRLDLVNAILPGLCIHCSLQGFEVPDNKNSKKMMH
                     YFSQRTSEAAIRCEFVKFQDRWEVILADEHGIIADDMISRYALSEKSQVELSTQVIKS
                     ASSKSVNKSDIDTSVFLNWYNPEKKMIRAYATVIDGPEYFWCQFADTEKLQCLEVEVQ
                     TAGEQVADRRNCIPCPYIGDPCIVRYREDGHYYRALITNICEDYLVSVRLVDFGNIED
                     CVDPKALWAIPSELLSVPMQAFPCCLSGFNISEGLCSQEGNDYFYEIITEDVLEITIL
                     EIRRDVCDIPLAIVDLKSKGKSINEKMEKYSKTGIKSALPYENIDSEIKQTLGSYNLD
                     VGLKKLSNKAVQNKIYMEQQTDELAEITEKDVNIIGTKPSNFRDPKTDNICEGFENPC
                     KDKIDTEELEGELECHLVDKAEFDDKYLITGFNTLLPHANETKEILELNSLEVPLSPD
                     DESKEFLELESIELQNSLVVDEEKGELSPVPPNVPLSQECVTKGAMELFTLQLPLSCE
                     AEKQPELELPTAQLPLDDKMDPLSLGVSQKAQESMCTEDMRKSSCVESFDDQRRMSLH
                     LHGADCDPKTQNEMNICEEEFVEYKNRDAISALMPLFSEEESSDGSKHNNGLPDHISA
                     QLQNTYTLKAFTVGSKCVVWSSLRNTWSKCEILETAEEGTRVLNLSNGMEEIVNPENV
                     WNGIPKLDKSPPEKRGLEVMEI"
     exon            6291..6415
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /inference="alignment:Splign:2.1.0"
     exon            6416..6505
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /inference="alignment:Splign:2.1.0"
     exon            6506..8967
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7041..7046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="hexamer: AATAAA"
     regulatory      7058..7063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="hexamer: AATAAA"
     polyA_site      7063
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="major polyA site"
     polyA_site      7077
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
     regulatory      8379..8384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="hexamer: AATAAA"
     polyA_site      8410
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
     regulatory      8945..8950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
                     /note="hexamer: ATTAAA"
     polyA_site      8967
                     /gene="TDRD6"
                     /gene_synonym="bA446F17.4; CT41.2; NY-CO-45; SPATA36;
                     TDR2"
ORIGIN      
        1 gtgcccggaa gcgcgacctc gggcggttgg aggggctacc gggtcttacc agtccgtggc
       61 gggagtcccg gaggaccctc gacgggggag ttgccgagaa aaggcctcgc cggcattctt
      121 cccctccact gggtcctttg aacctagttt ggctgggact cgccttcagg cggcgcggag
      181 gatttcgagg ccctgaggcg cggcccttaa tttccggaag tgggggccgc gccgcgccgt
      241 caagatgtgc tcgacgcccg gaatgccggc gccgggggcc tcgctggccc tgcgggtgtc
      301 cttcgtggac gtgcatcccg atgtgatccc ggtgcagctg tgggggctgg tgggcgagcg
      361 gcggggcgag tacctgcggc tgagccggga aatccaggaa gcggcggcca cgcgcggcca
      421 gtgggcgctg ggcagcgcct cggcctcgcc cggcgagctg tgcctggtgc aggtcgggct
      481 tttgtggcac cgctgccgcg tggtcagccg gcaggcacag gagagccgtg tcttcctgct
      541 ggacgagggc cgcaccatca cggccggagc aggctcgctg gcgcctgggc gcagagagtt
      601 cttcaatttg ccctcggaag tgctgggctg cgtgctagcg ggcctggtgc cggcaggctg
      661 cggcgcgggc tcaggcgagc cgccgcagca ctggcccgcc gacgccgtgg acttccttag
      721 caaccttcag ggcaaggagg tgcacgggtg cgtcctggac gtgctgctgc tccatcgcct
      781 ggtcctcctg gaggtgcctg atgtgttcca acagatgcgg gagctgggcc tggctcggcg
      841 ggtgcccgac agcctcttcc gttcgctgct ggagcgctat ctcacagcgg ccactgctag
      901 cgtgggctcc ggggtcccgg ttctctcgcg agtcccgctc aagcaaaagc agcctggtct
      961 ggattacttc tatccccagc tgcagctggg cgtgacggag gccgtggtca taacccaagt
     1021 gtgccatccc caccgcattc actgccagct ccgcagcgtc tcgcaggaga tccaccgcct
     1081 ctccgagagc atggcccagg tataccgggg ttccacgggg acaggggatg agaactctac
     1141 cagtgccacc tgggaggaga gggaggagag cccagataag ccgggctctc cgtgtgcatc
     1201 ctgtggcctg gatggacatt ggtacagagc actgttgctt gagacttttc ggccccagcg
     1261 ctgtgcccag gtgcttcatg tggactatgg aaggaaggag ttagtgagtt gcagcagcct
     1321 tcggtacttg ctgcctgaat attttcgaat gccggtggtg acctaccctt gtgctttgta
     1381 tggactctgg gacggtggga gaggctggtc tcggtcacag gtcggtgacc tgaagacact
     1441 gatactaggc aaggcagtga atgcaaagat tgaattttat tgctcctttg agcatgtgta
     1501 ttatgtcagc ctgtatggag aagatgggat taatctgaac cgtgtgtttg gagtacagtc
     1561 gtgttgcttg gctgaccgag tccttcagag ccaggcaaca gaggaggagg aaccagaaac
     1621 atctcagtct cagtctcctg ctgaagaagt agatgaagag atttcactcc cagccttaag
     1681 atctatcagg ttaaagatga atgccttcta cgatgcgcag gtagagtttg ttaaaaatcc
     1741 ttctgagttt tggattaggt tgaggaaaca caatgtcacc ttcagtaagc tgatgaggag
     1801 aatgtgtggt ttctattcct ctgccagtaa gctggatggt gtagttttga aacctgaacc
     1861 tgatgacctt tgctgtgtca agtggaaaga aaatggttat tatagggcca tagtcaccaa
     1921 attggatgac aagagtgtgg atgtattctt agttgaccga ggcaattcgg aaaatgtgga
     1981 ctggtatgac gtaaggatgc tgcttcctca gtttaggcag ctaccaatat tggctgtgaa
     2041 gtgcaccctg gctgatattt ggcctttggg aaaaacttgg agccaggagg cagtttcctt
     2101 ttttaaaaag actgtgctcc acaaagaatt agtcatccat attcttgata aacaggatca
     2161 tcaatatgtt attgagattc ttgacgaatc aagaacaggg gaagaaaaca ttagtaaggt
     2221 aattgcccaa gctggatatg ccaagtatca ggaatttgaa acaaaggaaa atatcctggt
     2281 aaatgcccac tccccagggc atgtttcaaa ccactttact acggagagta acaaaatacc
     2341 ttttgccaag actggagaag gagagcagaa agccaagaga gagaataaaa ccacatctgt
     2401 ttcaaaagct ttgagtgaca caacagttgt aacaaatggt tcaactgaac tagttgtgca
     2461 ggaaaaagtg aaaagagcat ctgtttattt tcctcttatg cagaattgct tggaaattaa
     2521 gccaggctcc tctagtaaag gagagctgga agttggaagt acagtagaag tcagagtgtc
     2581 ttatgttgaa aaccctggct atttctggtg tcagctgacc aggaacatac aaggacttaa
     2641 aactctaatg tctgatattc agtactattg caaaaataca gctgctcctc accagagaaa
     2701 cacccttgct tgtttggcta agcgaacagt aaacagacag tggtccagag cacttattag
     2761 tgggatacag tctgtggagc atgtcaatgt aacatttgta gattatggag acagagaaat
     2821 ggtatctgtg aagaatattt attcaattag tgaagaattt ctgaaggtta aggcacaggc
     2881 ttttaggtgc agtctttata atttaattca accagttggc cagaatccct ttgtttggga
     2941 tgtaaaggca atacaagctt tcaatgaatt tatagataat gcatggcaaa aaaatctaga
     3001 attaaaatgt acaatatttg ctctggcttc aattaatgaa gaactgttta acattgtgga
     3061 tttgctaacc ccctttcaga gtgcatgcca tttcttggta gaaaagagac ttgcaagacc
     3121 agtaaaactt cagaagcctt tggagtcctc tgttcagcta cattcctact tctattctac
     3181 acatgatatg aaaattggaa gtgaagaatt agtttatata acgcatattg atgacccttg
     3241 gacattttat tgccagctgg caagaaatgc aaatatttta gaacagttgt catgtagtat
     3301 tacacaatta agtaaagttt tgctgaattt aaaaacatct cccttgaacc ctggaacctt
     3361 gtgccttgcc aagtatactg atggaaactg gtataggggc atagtaatag agaaagagcc
     3421 aaagaaagtc ttctttgttg attttgggaa tatttatgta gtaacaagtg atgatctgct
     3481 tccaatacct agtgatgcat atgatgtctt acttttgccc atgcaagctg tcagatgttc
     3541 attatctgat attcctgatc atataccaga agaagtggtg gtgtggtttc aggagactat
     3601 tttagataag tcattgaagg ctttagttgt agcaaaagat ccagatggaa cactgattat
     3661 agaactatat ggtgacaata ttcaaattag tgctagtatt aataagaagt tggggctact
     3721 tagttacaaa gatagaataa gaaaaaaaga aagtgaagtc ctctgttcta caactgaaac
     3781 tcttgaagaa aaaaatgaga atatgaagtt gccatgtaca gagtatttaa gtaaatcagt
     3841 agggtacaag ttacctaata aagaaatttt ggaagagtca tataaacctc agatcaactc
     3901 atcatacaag gaactcaaac ttttacaaag tttaacaaaa acaaacttag tcactcaata
     3961 tcaagactct gtgggaaata aaaatagtca agtgtttcca ttaacaacag aaaagaaaga
     4021 agaaatttct gctgagacac ccttgaaaac agcaagagta gaagctactc tttcagagag
     4081 aaaaatagga gattcatgtg acaaagattt gcctctgaaa ttttgtgagt tcccacagaa
     4141 gactataatg cctggattta aaacaactgt atatgtttct catataaatg acctttcaga
     4201 cttttatgtt caactaatag aagatgaagc tgaaattagt catctttcag agagattaaa
     4261 cagtgttaaa acaaggcccg aatattatgt aggtccacct ttgcaaagag gagatatgat
     4321 atgtgctgtt ttcccagaag ataatttatg gtatcgtgct gtgatcaagg agcaacaacc
     4381 caatgacctt ctctctgtgc agtttataga ttatggcaat gtttctgtgg ttcatactaa
     4441 caaaataggt aggcttgacc ttgttaatgc aatattgccg gggttgtgca ttcattgctc
     4501 cttgcaggga tttgaggttc ctgacaataa aaattctaag aaaatgatgc attacttttc
     4561 ccaacggacc agcgaggctg caataagatg tgaatttgtt aaatttcaag acagatggga
     4621 agttattctt gctgatgaac atgggatcat agcagatgat atgattagca ggtatgctct
     4681 cagtgaaaaa tctcaagtag aactttctac ccaagtaatt aaaagtgcca gttcaaagtc
     4741 tgttaacaaa tcagacattg acacttcagt atttcttaac tggtataatc cagaaaaaaa
     4801 aatgataaga gcttatgcca ctgtgataga tggacctgag tacttttggt gtcagtttgc
     4861 tgatacggag aaacttcagt gtttagaagt agaagtacag actgctggag aacaggtagc
     4921 agacaggaga aattgtatcc catgtcctta tattggagat ccttgtatag taagatacag
     4981 agaagatgga cattattata gggcacttat cactaatatt tgtgaagatt atcttgtatc
     5041 tgtcaggctt gtggactttg gaaacattga agactgtgtg gacccaaaag cactctgggc
     5101 cattccttct gaacttctgt cggttcccat gcaagccttt ccatgttgcc tctcagggtt
     5161 taacatttca gaaggattat gttctcaaga gggaaatgac tatttctatg aaataataac
     5221 agaagatgtg ttggaaataa caatactaga aatcagaagg gatgtttgtg atatcccttt
     5281 agcaattgtt gacttgaaaa gcaaaggtaa aagtattaat gagaaaatgg agaaatattc
     5341 taagactggt attaaaagtg ctcttcccta tgaaaatatt gactcagaga taaagcagac
     5401 tcttgggtcc tacaatcttg atgtaggact taagaaatta agtaataaag ctgtacaaaa
     5461 taaaatatat atggaacaac agacagatga gcttgctgaa ataactgaaa aagatgtaaa
     5521 cattattgga accaaaccaa gtaacttccg tgaccctaaa actgataaca tttgtgaagg
     5581 gtttgaaaac ccctgcaaag ataaaattga tactgaggaa ctggaaggtg aattagagtg
     5641 ccatctggtt gacaaagcag agtttgatga taaatacctg attacaggat ttaacacatt
     5701 actaccacat gctaatgaaa caaaggagat actagaactg aattcacttg aggtgccgct
     5761 ttctcctgat gatgaatcaa aagaattctt agaactggaa tctattgagt tacagaattc
     5821 tctggtggtg gatgaagaaa aaggggagct aagcccggtg ccaccgaatg tgccactctc
     5881 ccaagagtgt gtcacaaaag gcgccatgga gctatttaca ctgcagcttc ctctcagctg
     5941 tgaagctgag aaacagccag aactagaact acctacagcc cagctgcctt tagatgacaa
     6001 gatggatcct ttgtctttag gagttagtca gaaagcacag gaatccatgt gtactgagga
     6061 catgagaaag tcaagttgtg tagaatcttt tgatgaccag cgcaggatgt cattgcatct
     6121 acatggagca gattgtgatc ctaaaacaca gaatgaaatg aatatatgtg aagaagaatt
     6181 tgtagagtat aaaaacaggg atgccatttc ggcattgatg cctttgttct ctgaggaaga
     6241 aagcagtgat ggaagcaagc acaataatgg tttaccagat catatctcag ctcaactaca
     6301 gaacacctac actctgaaag cctttactgt tggatctaaa tgtgttgtgt ggtcaagtct
     6361 aagaaacaca tggtctaaat gtgagatttt agaaacagct gaagaaggaa caagggtttt
     6421 gaacctttca aatggtatgg aggagatagt gaaccctgag aatgtctgga atggcatacc
     6481 caaattggat aagagtccac ctgagaaaag gggtttggag gtgatggaga tttaaccgtg
     6541 gatctatagc tgtggccaat cagtcagaag ctgcccttga acaagtggca tcttacgcag
     6601 accaacagag tatttgagaa aattgaaaac atgtaaccac aagaagttgt cattttcaaa
     6661 aacttctata taggtggaaa acaaattagg tctcaggttg atggtggggt gtgtttatag
     6721 tgatcctgtt atatatacag atctgggatc tttcgtcttt attgtcttac gtttctaatt
     6781 agttgggagg atttattttg ctaaacagtt tactaacaca ttacatttca aaaactattt
     6841 tggtaccttt caaatacagt gtttaaatta aaatagaaaa ataagggctc atgacaagta
     6901 cattatttga ttctacttag gatagctttt tagcaggatc tccttcagaa tttttgtctt
     6961 gactttgaat ctttgcctgt ttgtctaaac atttgactaa cattctgttt gaatttggaa
     7021 gtattctaat acaagatttg aataaagttt atccttaaat aaagtttatc cttaatatga
     7081 tggtctcatt ttattaaaaa ggaaatagaa ctcttaaata tacttggttc catctctcct
     7141 ctgtaatgga ggattgggga aagcatagta atattaaatg ttagctctta tgtattagtc
     7201 acttagctta tactttaaat gcattttttc actttattca tcaactttga agtgcatatc
     7261 aatattatcc atattttata ggtgagaaaa ttgaagcttc ataaagaggt taaataactt
     7321 gcctaaggtc agatagctga tgagtctcag aggcttgatc aaacccagac ctacctaact
     7381 ttgaagccca tgacttttta aacatgattt tgcaaagcat agatcttagc ttttagaaac
     7441 agatctgagc tatatgctgc ttaacagata tgtgacatgg gcaaattatg taatattttg
     7501 aactttgatc ttcatctgta aagtggtgat aataaaacct ttcttgcagt gtttttttaa
     7561 gatgagaaat aattgatata aagtccctga aaaggagtag gcatttgata aatgtgtctt
     7621 tctttaccct gaaaaatact cagtgtgcac tatgttaggt tatttctttt taaccttaac
     7681 ttattagata ttttcaatag ttattttctc ctttcagcat cagcatgcgc caccaacaaa
     7741 agactgtaaa aaacaaacaa acaaaaaagt gttgaatggg acatccatac agttggaatg
     7801 cttatttcta aaatgaatag ggaaaaggtt tgctaaatga gactggggta aaggtgtatc
     7861 tctgtgaagc attatcagac tctgaagcct aatggaaata gaattatgaa ccttaaaaac
     7921 ccattataat ttccgtaagt agaagggaga gaggtttgca ttaaaagtaa ttgaggtaga
     7981 tgagtatatt acttattcct ggaataatga atagacaagt ttggcttact catatagata
     8041 atataatgtg aattgccaga agcaaagctc agagaagatg cattaggatc agattatagg
     8101 aagccttcaa tttcaagctg agtttaactc cctattttaa aaaatttaat ctgaatggct
     8161 acagaagtaa aatttctcag aatgagtgac tttgcacctg acaccactaa gataggacta
     8221 gggtaccaaa tgagtggtat aaatttttat gtcccctggg aaataaaatc attttccatg
     8281 attcccatat ttataagtat aaattcttaa gctatatttt tacctccatt taatcgtgtt
     8341 attctgtttt actactacat atagaattga aaacattaaa taaaattaac tggaatatga
     8401 agaagctaaa tgtaattctg aatgttttat tatccttcac agcctttgtg tgagtaacta
     8461 atgtattcta actggaatcc ctgctatatg ctgtaatacc tgtggaacct ttctttagca
     8521 agtagaatgc cttaaagaat taaagaaagt gtcaaaaaat taccaattta atccactgcc
     8581 tttgcctcat ctctaatatt cttaaccctt ctctactcct tttagtcatc atttgaaatt
     8641 gtgtagtacc accaatcctg tgctgaaaca atggcttttg cttgttctaa tttttcttac
     8701 cgtgaccaaa agtcaaagag aagagctgcc aaattctacc tagaatatca tgttgctata
     8761 attaaactcc actgcccata gtagataata aaaggcaaga aattgttttt tactctatgt
     8821 ttagggagtc ttgtatcatt gcaacaggtt ttcactggga ttttgcctgt tttaatctac
     8881 ttgtctgtca aagtaatgta aaaacagttt ttaacttcga cactgaaaag gaatcatctt
     8941 taaaattaaa atttaggctg atatgaa
//
