LOCUS       NM_001395205            8149 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens tudor domain containing 1 (TDRD1), transcript variant
            13, mRNA.
ACCESSION   NM_001395205
VERSION     NM_001395205.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Kim,H., Barua,A., Huang,L., Zhou,T., Bolaji,M., Zachariah,S.,
            Mitra,A., Jung,S.Y., He,B. and Feng,Q.
  TITLE     The cancer testis antigen TDRD1 regulates prostate cancer
            proliferation by associating with the snRNP biogenesis machinery
  JOURNAL   Oncogene 42 (22), 1821-1831 (2023)
   PUBMED   37041411
  REMARK    GeneRIF: The cancer testis antigen TDRD1 regulates prostate cancer
            proliferation by associating with the snRNP biogenesis machinery.
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Busetto,G.M., Del Giudice,F., Maggi,M., De Marco,F., Porreca,A.,
            Sperduti,I., Magliocca,F.M., Salciccia,S., Chung,B.I., De
            Berardinis,E. and Sciarra,A.
  TITLE     Prospective assessment of two-gene urinary test with
            multiparametric magnetic resonance imaging of the prostate for men
            undergoing primary prostate biopsy
  JOURNAL   World J Urol 39 (6), 1869-1877 (2021)
   PUBMED   32681273
  REMARK    GeneRIF: Prospective assessment of two-gene urinary test with
            multiparametric magnetic resonance imaging of the prostate for men
            undergoing primary prostate biopsy.
REFERENCE   3  (bases 1 to 8149)
  AUTHORS   Mo,H.Y., Choi,E.J., Yoo,N.J. and Lee,S.H.
  TITLE     Mutational alterations of TDRD 1, 4 and 9 genes in colorectal
            cancers
  JOURNAL   Pathol Oncol Res 26 (3), 2007-2008 (2020)
   PUBMED   32036563
  REMARK    GeneRIF: Mutational alterations of TDRD 1, 4 and 9 genes in
            colorectal cancers.
REFERENCE   4  (bases 1 to 8149)
  AUTHORS   Babakhanzadeh,E., Khodadadian,A., Rostami,S., Alipourfard,I.,
            Aghaei,M., Nazari,M., Hosseinnia,M., Mehrjardi,M.Y.V., Jamshidi,Y.
            and Ghasemi,N.
  TITLE     Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in
            azoospermia
  JOURNAL   BMC Med Genet 21 (1), 33 (2020)
   PUBMED   32059713
  REMARK    GeneRIF: Here we evaluate gene and protein expression levels of
            four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular
            biopsy samples obtained from men with obstructive azoospermia (OA,
            n = 29), as controls, and various types of non-obstructive
            azoospermia containing hypospermatogenesis (HP, 28), maturation
            arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32)
            as cases.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8149)
  AUTHORS   Xiao,L., Lanz,R.B., Frolov,A., Castro,P.D., Zhang,Z., Dong,B.,
            Xue,W., Jung,S.Y., Lydon,J.P., Edwards,D.P., Mancini,M.A., Feng,Q.,
            Ittmann,M.M. and He,B.
  TITLE     The Germ Cell Gene TDRD1 as an ERG Target Gene and a Novel Prostate
            Cancer Biomarker
  JOURNAL   Prostate 76 (14), 1271-1284 (2016)
   PUBMED   27272765
  REMARK    GeneRIF: TDRD1 protein is expressed in the majority of human
            prostate tumors, but not in normal prostate tissue.
REFERENCE   6  (bases 1 to 8149)
  AUTHORS   Boormans,J.L., Korsten,H., Ziel-van der Made,A.J., van
            Leenders,G.J., de Vos,C.V., Jenster,G. and Trapman,J.
  TITLE     Identification of TDRD1 as a direct target gene of ERG in primary
            prostate cancer
  JOURNAL   Int J Cancer 133 (2), 335-345 (2013)
   PUBMED   23319146
  REMARK    GeneRIF: TDRD1 is the first identified upregulated direct ERG
            target gene that is strongly associated with ERG overexpression in
            primary prostate cancer
REFERENCE   7  (bases 1 to 8149)
  AUTHORS   Mathioudakis,N., Palencia,A., Kadlec,J., Round,A., Tripsianes,K.,
            Sattler,M., Pillai,R.S. and Cusack,S.
  TITLE     The multiple Tudor domain-containing protein TDRD1 is a molecular
            scaffold for mouse Piwi proteins and piRNA biogenesis factors
  JOURNAL   RNA 18 (11), 2056-2072 (2012)
   PUBMED   22996915
REFERENCE   8  (bases 1 to 8149)
  AUTHORS   Kojima,K., Kuramochi-Miyagawa,S., Chuma,S., Tanaka,T.,
            Nakatsuji,N., Kimura,T. and Nakano,T.
  TITLE     Associations between PIWI proteins and TDRD1/MTR-1 are critical for
            integrated subcellular localization in murine male germ cells
  JOURNAL   Genes Cells 14 (10), 1155-1165 (2009)
   PUBMED   19735482
REFERENCE   9  (bases 1 to 8149)
  AUTHORS   Reuter,M., Chuma,S., Tanaka,T., Franz,T., Stark,A. and Pillai,R.S.
  TITLE     Loss of the Mili-interacting Tudor domain-containing protein-1
            activates transposons and alters the Mili-associated small RNA
            profile
  JOURNAL   Nat Struct Mol Biol 16 (6), 639-646 (2009)
   PUBMED   19465913
REFERENCE   10 (bases 1 to 8149)
  AUTHORS   Wang,P.J., McCarrey,J.R., Yang,F. and Page,D.C.
  TITLE     An abundance of X-linked genes expressed in spermatogonia
  JOURNAL   Nat Genet 27 (4), 422-426 (2001)
   PUBMED   11279525
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022023.7.
            
            Summary: This gene encodes a protein containing a tudor domain that
            is thought to function in the suppression of transposable elements
            during spermatogenesis. The related protein in mouse forms a
            complex with piRNAs and Piwi proteins to promote methylation and
            silencing of target sequences. This gene was observed to be
            upregulated by ETS transcription factor ERG in prostate tumors.
            [provided by RefSeq, Sep 2018].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.2466087.1,
                                           SRR14038193.332006.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000695399.1/ ENSP00000511878.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                AC022023.7         65211-65274         c
            65-395              AC022023.7         56471-56801         c
            396-454             AC022023.7         53608-53666         c
            455-599             AC022023.7         45310-45454         c
            600-705             AC022023.7         43112-43217         c
            706-766             AC022023.7         42329-42389         c
            767-871             AC022023.7         41451-41555         c
            872-1051            AC022023.7         41060-41239         c
            1052-1195           AC022023.7         40411-40554         c
            1196-1367           AC022023.7         39734-39905         c
            1368-1454           AC022023.7         38297-38383         c
            1455-1622           AC022023.7         33879-34046         c
            1623-1730           AC022023.7         33660-33767         c
            1731-1901           AC022023.7         32591-32761         c
            1902-2144           AC022023.7         31039-31281         c
            2145-2282           AC022023.7         30513-30650         c
            2283-2393           AC022023.7         26972-27082         c
            2394-2564           AC022023.7         26043-26213         c
            2565-2840           AC022023.7         23784-24059         c
            2841-2960           AC022023.7         23151-23270         c
            2961-3077           AC022023.7         21924-22040         c
            3078-3245           AC022023.7         18411-18578         c
            3246-3473           AC022023.7         17328-17555         c
            3474-3520           AC022023.7         16671-16717         c
            3521-8149           AC022023.7         11961-16589         c
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q25.3"
     gene            1..8149
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /note="tudor domain containing 1"
                     /db_xref="GeneID:56165"
                     /db_xref="HGNC:HGNC:11712"
                     /db_xref="MIM:605796"
     exon            1..64
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            65..395
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..3613
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /note="isoform 3 is encoded by transcript variant 13;
                     cancer/testis antigen 41.1; tudor domain-containing
                     protein 1; testicular tissue protein Li 193"
                     /codon_start=1
                     /product="tudor domain-containing protein 1 isoform 3"
                     /protein_id="NP_001382134.1"
                     /db_xref="CCDS:CCDS91352.1"
                     /db_xref="GeneID:56165"
                     /db_xref="HGNC:HGNC:11712"
                     /db_xref="MIM:605796"
                     /translation="MSVKSPFNVMSRNNLEAPPCKMTEPFNFEKNENKLPPHESLRSP
                     GTLPNHPNFRLKSSENGNKKNNFLLCEQTKQYLASQEDNSVSSNPNGINGEVVGSKGD
                     RKKLPAGNSVSPPSAESNSPPKEVNIKPGNNVRPAKSKKLNKLVENSLSISNPGLFTS
                     LGPPLRSTTCHRCGLFGSLRCSQCKQTYYCSTACQRRDWSAHSIVCRPVQPNFHKLEN
                     KSSIETKDVEVNNKSDCPLGVTKEIAIWAERIMFSDLRSLQLKKTMEIKGTVTEFKHP
                     GDFYVQLYSSEVLEYMNQLSASLKETYANVHEKDYIPVKGEVCIAKYTVDQTWNRAII
                     QNVDVQQKKAHVLYIDYGNEEIIPLNRIYHLNRNIDLFPPCAIKCFVANVIPAEGNWS
                     SDCIKATKPLLMEQYCSIKIVDILEEEVVTFAVEVELPNSGKLLDHVLIEMGYGLKPS
                     GQDSKKENADQSDPEDVGKMTTENNIVVDKSDLIPKVLTLNVGDEFCGVVAHIQTPED
                     FFCQQLQSGRKLAELQASLSKYCDQLPPRSDFYPAIGDICCAQFSEDDQWYRASVLAY
                     ASEESVLVGYVDYGNFEILSLMRLCPIIPKLLELPMQAIKCVLAGVKPSLGIWTPEAI
                     CLMKKLVQNKIITVKVVDKLENSSLVELIDKSETPHVSVSKVLLDAGFAVGEQSMVTD
                     KPSDVKETSVPLGVEGKVNPLEWTWVELGVDQTVDVVVCVIYSPGEFYCHVLKEDALK
                     KLNDLNKSLAEHCQQKLPNGFKAEIGQPCCAFFAGDGSWYRALVKEILPNGHVKVHFV
                     DYGNIEEVTADELRMISSTFLNLPFQGIRCQLADIQSRNKHWSEEAITRFQMCVAGIK
                     LQARVVEVTENGIGVELTDLSTCYPRIISDVLIDEHLVLKSASPHKDLPNDRLVNKHE
                     LQVHVQGLQATSSAEQWKTIELPVDKTIQANVLEIISPNLFYALPKGMPENQEKLCML
                     TAELLEYCNAPKSRPPYRPRIGDACCAKYTSDDFWYRAVVLGTSDTDVEVLYADYGNI
                     ETLPLCRVQPITSSHLALPFQIIRCSLEGLMELNGSSSQLIIMLLKNFMLNQNVMLSV
                     KGITKNVHTVSVEKCSENGTVDVADKLVTFGLAKNITPQRQSALNTEKMYRMNCCCTE
                     LQKQVEKHEHILLFLLNNSTNQNKFIEMKKLLKS"
     exon            396..454
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            455..599
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            600..705
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..766
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            767..871
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1051
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1195
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1367
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1454
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1622
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1730
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1901
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2144
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2282
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2393
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2564
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2840
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2960
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3077
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3078..3245
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3246..3473
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3520
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3521..8149
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8127..8132
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /note="hexamer: AATAAA"
     polyA_site      8149
                     /gene="TDRD1"
                     /gene_synonym="CT41.1"
                     /note="major polyA site"
ORIGIN      
        1 agtcgccatc accgccagac cgcagagaca ggttcggatc cgcggtcctc ttgcctcttt
       61 ccaggcctcg atgagtgtta aatcgccatt taatgtgatg tcaagaaata atttggaagc
      121 acctccttgt aagatgacag agccatttaa ttttgagaaa aatgaaaaca agcttccacc
      181 acatgagtct ttaagaagtc ctggaacact tcctaaccac cctaatttca ggctgaaaag
      241 ctcagagaat ggaaataaaa agaacaattt tttgctttgt gagcaaacca aacaatattt
      301 ggctagtcag gaagacaatt cagtttcttc aaacccgaat ggcatcaacg gagaagtagt
      361 tggctccaaa ggagacagga aaaaattgcc agcaggaaac tcagtgtcac caccaagtgc
      421 tgaaagtaat tcaccaccca aagaagtgaa tattaagcct ggaaataatg tacgtcctgc
      481 aaaatcaaaa aaactaaaca agttggtcga gaattccttg tccataagta atccagggct
      541 cttcacctcc ttaggacctc ctcttcggtc cacaacttgc catcgctgtg gcctatttgg
      601 atcgctgagg tgctctcagt gcaagcagac ctactattgc tccacagcat gtcaaagaag
      661 agactggtct gcacacagca tcgtgtgcag gcctgttcag ccaaatttcc acaaacttga
      721 aaataaatca tctattgaaa caaaggatgt ggaggtaaac aataagagtg actgtccact
      781 tggagttact aaggaaatag ccatttgggc tgagagaata atgttttctg atttgagaag
      841 tctacaactc aagaaaacca tggaaataaa gggtacggtt accgaattca aacacccagg
      901 ggacttctac gtgcagttat attcttcaga agttttagaa tacatgaacc aactctctgc
      961 cagcttaaaa gaaacatatg caaatgtgca tgaaaaagac tatattcctg ttaaggggga
     1021 agtttgtatt gccaagtaca ctgttgatca gacctggaac agagcaatca tacaaaacgt
     1081 tgatgtgcag caaaagaagg cacatgtctt atatattgat tatggaaatg aagaaataat
     1141 tccattaaac agaatttacc acctcaacag gaacattgac ttgtttcctc cttgtgccat
     1201 aaagtgcttt gtagccaatg ttatcccagc agaagggaat tggagcagtg attgtatcaa
     1261 agctactaaa ccactgttaa tggagcagta ctgctccata aagattgtcg acatcttgga
     1321 agaggaagtg gttacctttg ctgtagaagt tgagctgcca aattcaggaa aacttttaga
     1381 ccatgtgctt atagaaatgg gatatggctt gaaacccagt ggacaagatt ctaagaagga
     1441 aaatgcagat caaagtgatc ctgaagatgt tggaaaaatg acaactgaaa acaacattgt
     1501 cgtagacaaa agtgacctaa tcccaaaagt gttaactttg aatgtaggtg atgagttttg
     1561 tggtgtggtt gcccacattc aaacaccaga agacttcttt tgtcaacaac tgcaaagtgg
     1621 ccgaaagctt gctgaacttc aggcatccct tagcaagtac tgtgatcagt tgcctccacg
     1681 ctctgatttt tatccagcca ttggtgatat atgttgtgct cagttctcag aggatgatca
     1741 gtggtaccgt gcctctgttt tggcttacgc ttctgaagaa tctgtactgg tcggatatgt
     1801 agattatgga aactttgaaa tccttagttt gatgagactt tgtcccataa tcccaaagtt
     1861 gttggaattg ccaatgcaag ctataaagtg tgtactagca ggagtaaagc catcattagg
     1921 aatttggact ccagaagcta tttgtctcat gaaaaaactt gtacagaaca aaataatcac
     1981 agtgaaagtg gtggacaagt tggaaaacag ttccctggtg gagcttattg ataaatccga
     2041 gacgcctcat gtcagtgtta gcaaagttct cctagatgca ggctttgctg tgggagaaca
     2101 gagtatggtg acagataaac ccagtgacgt gaaagaaacc agtgttccct tgggtgtgga
     2161 aggaaaagta aatccattgg agtggacatg ggttgaactt ggtgttgacc aaacagtaga
     2221 tgttgtggtc tgtgtgatat atagtcctgg agaattttat tgccatgtgc ttaaagagga
     2281 tgctttaaag aaactcaatg atttgaacaa gtcattagca gaacactgcc agcagaagtt
     2341 acctaatggt ttcaaggcag agataggaca accttgttgt gctttttttg caggtgatgg
     2401 tagttggtat cgtgctttag tcaaggaaat cttaccaaat ggacatgtta aagtacattt
     2461 tgtggattat ggaaacatcg aagaagttac tgcagatgaa ctccgaatga tatcatcaac
     2521 atttttaaac cttccctttc agggaatacg gtgccagtta gcagatatac agtctagaaa
     2581 caaacattgg tctgaagaag ccataacaag attccagatg tgtgttgctg ggataaaatt
     2641 gcaagccaga gtggttgaag tcactgaaaa tgggatagga gttgaactca ccgatctctc
     2701 cacttgttat cccagaataa ttagtgatgt tctgattgat gaacatctgg ttttaaaatc
     2761 tgcttcacca cataaagact taccaaatga cagacttgtt aataaacatg agcttcaagt
     2821 tcatgtacag ggacttcaag ctacctcttc agctgagcaa tggaagacga tagaattgcc
     2881 agtggataaa actatacaag caaatgtatt agaaatcata agcccaaact tgttttatgc
     2941 tctaccaaaa gggatgccag aaaatcagga aaagctgtgc atgttgacag ctgaattatt
     3001 agaatactgc aatgctccga aaagtcgacc accctataga ccaagaattg gagacgcatg
     3061 ctgtgccaaa tacacaagtg atgatttttg gtatcgtgca gttgttctgg ggacatcaga
     3121 cactgatgtg gaagtgctct atgcagacta tggaaacatt gaaaccctgc ctctttgcag
     3181 agtgcaacca atcacctcta gccacctggc gcttcctttc caaattatta gatgttcact
     3241 tgaaggatta atggaattga atggaagctc ttctcaatta ataataatgc tattaaaaaa
     3301 tttcatgttg aatcagaatg taatgctttc tgtgaaagga attacaaaga atgtccatac
     3361 agtgtcagtt gagaaatgtt ctgagaatgg gactgtcgat gtagctgata agctagtgac
     3421 atttggtctg gcaaaaaaca tcacacctca aaggcagagt gctttaaata cagaaaagat
     3481 gtataggatg aattgctgct gcacagagtt acagaaacaa gttgaaaaac atgaacatat
     3541 tcttctcttc ctcttaaaca attcaaccaa tcaaaataaa tttattgaaa tgaaaaaact
     3601 gttaaaaagt taagtaagtt aaatcgtatg ttttcgcctc ttctgtgatc accaatagga
     3661 catcttcagg catattggca ggatagagct aatggagtga aacctattgt aaggctgtac
     3721 tttcgtgatt taatgacctg aggtttggtc ataatgcttc tgctgttttt gtaggtttat
     3781 ctgatcgttt tcctttgcta ctgctaatgg aactgaaccc ccaggggtat tccagttgta
     3841 atagcctttc cttactgttg tttggttctg tgaatgccta tgttattgat atgtggaggg
     3901 acttgtaaaa cttgttgtga cataaagctt agcctcagat tttttgtcta gatctgaaat
     3961 taactcagag ctcacttatg cacattgctg taaatatgtt tagcactttt tgcgaggggt
     4021 atctaaaggg ccaagaacgg aaagtggatc taaagggcca agaacggaaa gtgggctgca
     4081 ctgagggtat ttttcattta aaaacaaaac atactccctg cttttggctg tggtgattct
     4141 agtgtctaaa gaacatactg gtgagtttta aaccagtgtt cctccccacc ccacccccat
     4201 attttaaatg tctgggatcc tttgttgtaa gatggttctc agaaataaat ttttgattaa
     4261 gagccaatta gaacttctga tcagggctgg gggtggtggc tcacgcctgt aatcccagca
     4321 ctttgggagg ctgaggcggg cagatcacct gagcccaggc gttcgaagtc agcctggcca
     4381 agatggcgaa acactgcctc tacttaaaat acaaaattag ctgggcatgg tggtgcacac
     4441 ctgttaaccc agctacttgg gaggctgaga caggagaatc gcttgaatcc aggaggcggc
     4501 ggttgcagtg agccaagact gctattatac tccagcctgg gtgacagagt gagagtttgt
     4561 ctcaaaaaca aaacaaaaca aaacaaaaaa aaaaacaacc tctaatctca gaataaagtt
     4621 gtctctcctc accactttga gttgttacag ttccagccaa aagcaaagga actttgattt
     4681 acttaatatg tgcacttttg ttggttaaca aatttatatt aaactgtttg gaaatagttc
     4741 aagaggtggc tataatactc ttcatgaatc ttttatgggt gtagtaactc tcaagtagga
     4801 accaactgcc aactgtgtta aaacatatct ttcatgtggt aatagaccgt ttctttgcgt
     4861 tttaagaaag gtcaattgaa ttataagcta aatgtaatta atgcaagatc aactatcttg
     4921 actctcatgt ttccttttac aaattttggg ttattacaac tttcagtcct ttctaataca
     4981 ttgtcttaat actgactgtt ttgtatgttt aagcttaaaa tcttttaatc tttttttttt
     5041 ttttttttgg agacggagtc ttgctctgtc acccaggctg gagtgcaatg gcgtgatctc
     5101 ggctcactgc aagctccacc tcccaggttc acgcaattct cccacatcag cctcccaagt
     5161 agctgggact acaggcgcac actgccacac ccggctaatc ttaaacttaa tggtgatttt
     5221 aatttcatag atcaaggaac atgcaatgac atggatttct ggaatttagt cctagtatca
     5281 agtcactata tatatatata tttttttttt attttttatt tttatttttt tttgagacag
     5341 tctcgctctg ttgtccaggc tggagtgcag tggcgctatc tcagctcact acaacctccg
     5401 cctcccgggt tcatgccatt ctcctgcctc agcttcccaa gtagctggga ctataggtgc
     5461 ccgccaccac acccagctaa tctttttgta tttttagtag agacagggtt tcaccgtgtt
     5521 agccaggatg gtctcgatct cctgacctcg tgatctgccc tcctcggcct cccaaagtgc
     5581 tgggattaca ggcgtgagcc actgcgcccg gccaccaagt cattacattt ttaaccaagt
     5641 tggttggcct gtatgttctt cttatctgtg taacaggcat ttttcctaag gatattgagt
     5701 gtgcttacac ataaaactta aagtgaaatt tcattgtttc ttgatagatt taagacaggg
     5761 cttagtagct ttcaaaagaa aacttgattt aaagcatagt agttgaactg gatttttcct
     5821 gactgtggga ataaatagat attttattta catttgaata cagtgcaata ccttgtgttt
     5881 gggtagtacg tatttacaaa gcatttttac atctactcac atacttaatc ttcacaatgc
     5941 ccttgaaaag aaagtaggac ccattttaca gttaaggaag ctgaggctca gagagggtta
     6001 catgactgtc caaaatcaca tacaggctga aacactaggt cttacgactt aaaacagttg
     6061 tgtcaacttc cctgagctgc tacgtgtaca gtatcctttt agatcagatc agttacatag
     6121 ggcatgaaac tagagggtta cattattagg aaaaaataat gtacatgatt gcttaagcat
     6181 tacttcattg tggtgaaaat agagctgaat atttactcct ttaaattatt ctttgagata
     6241 gaatgggtgt aaaaatgtat tttggctggg catggtggct caggcctgta atcccagcac
     6301 ttttgggagg ctgaggaggg caggtcacat gaggcccagg agtttgagac catcctggcc
     6361 aacatggtga aactttgtct ctaccaaaaa aaatatagaa ttagccaggt gaggtggcgt
     6421 gcacctgtag tcccagctac tggggaggct gaggcaggag aattgcttga acccaggagc
     6481 cgaaggttgc agtgagccga gatggcacca ctgcactcca gcttgggcaa cagagtgaga
     6541 ccctgtctca aaaaaataaa ataaaagaca aacacatgta tttcatcatc tggagaaatt
     6601 taattgttag caatttaatt gtaaaattgc taaaacagaa gtgctgtggt gatatgaaca
     6661 taaaaccctc tgtaatctgt tgctcaggtg atgtttcaac acacaagtta atgtcagata
     6721 tcttttatat ataaggagag tgcaaacaac tatatgttat atttgtgaag gggagtataa
     6781 acagctgtac ttagcatata ttatgctatg ttatggtggt cattaatttt cttaactgac
     6841 aggtctggct taaatgcgta atttcagtta aaagtgaata ttttgtttgc ttgtatttga
     6901 aattaaaagc catagcaatt gagtttttgt gtaaggcata atgatagttg attttttttc
     6961 tttttcagaa acagcatctc ttggaggtaa acccttatga gacaggaaac agcaaaggct
     7021 agctttagga gagaaagtac agcacctggt gtttttattt atgagaacct tttctttgtc
     7081 cactttctct gtaatgacct tctatccctc cgtttttgcc tgcctgccat tctcctatta
     7141 ggttggtggt ttttattttc ctctaagttc cttccaccaa ataaatatta cgtaaaaaat
     7201 tcataccaaa tcaatgagaa tactggcaag gaatacatag ggactttctg ctatatatgt
     7261 aactttttat tacttaaagg taccgaagga aggccaggtg cagtggctca cgcccagcac
     7321 tttgggaggc tgaggtggga ggatcccttg aggccaggag ttcaaggtta cagtgagcta
     7381 tgatagtgcc actgcactcc agcctgggtg acagattttg tcttaaaaaa aaaaaaaaaa
     7441 aagttgatat gagttttatt ttctgtccgt ttgaaatatt ttgtaatatt ccctgcattc
     7501 tctgtcgtct gcctcttcca cataatgtcc tttgctttca tgtttgttat cttctttttc
     7561 tgttcactca gaggtcatca atttctttct ctccgtcctt aattggatta tttttctttt
     7621 ggcctttggg cacagagtct gacctctgga ccactctaac tggagaagga actttatgtt
     7681 ccctctcctg ctgtgtccac aaccttagaa atctgtagct agatttttgt tgttatagat
     7741 agaatttact gtttctgaaa cccaaataca gttatcagtt taaggttaaa aaaaaaaaaa
     7801 aaacgaagag tgtatatttt tcctgaagca tgaaaataat ctcaaattct cctaaaccac
     7861 gcatatgcag aattatgggc tggctgactt tcctgccaac cccaaagttt gaattttttt
     7921 catgatttaa ctgtcagact atgaaaccta agatgtttct caccaactca gcagatactt
     7981 gaaagacttt tttttttttt tttttttttt ttgcttttac tggcaatgta cgttgtctaa
     8041 tctgttaaat gggatatttc ctcatgtaaa aagttgttaa aacactgatg tgcttctgtt
     8101 agataaaagc tcacgctgct ttgcccaata aaattatttc acttcccaa
//
