LOCUS       NM_001371623            5026 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1),
            transcript variant 8, mRNA.
ACCESSION   NM_001371623 XM_005268505
VERSION     NM_001371623.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Qu,Y., Meng,B., Cai,S., Yang,B., He,Y., Fu,C., Li,X., Li,P.,
            Cao,Z., Mao,X., Teng,W. and Shi,S.
  TITLE     Apoptotic metabolites ameliorate bone aging phenotypes via
            TCOF1/FLVCR1-mediated mitochondrial homeostasis
  JOURNAL   J Nanobiotechnology 22 (1), 549 (2024)
   PUBMED   39237990
  REMARK    GeneRIF: Apoptotic metabolites ameliorate bone aging phenotypes via
            TCOF1/FLVCR1-mediated mitochondrial homeostasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Gil Rosas,M., Centola,C., Torres,M., Mouguelar,V.S., David,A.P.,
            Piga,E.J., Gomez,D., Calcaterra,N.B., Armas,P. and Coux,G.
  TITLE     The transcription of the main gene associated with Treacher-Collins
            syndrome (TCOF1) is regulated by G-quadruplexes and cellular
            nucleic acid binding protein (CNBP)
  JOURNAL   Sci Rep 14 (1), 7472 (2024)
   PUBMED   38553547
  REMARK    GeneRIF: The transcription of the main gene associated with
            Treacher-Collins syndrome (TCOF1) is regulated by G-quadruplexes
            and cellular nucleic acid binding protein (CNBP).
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5026)
  AUTHORS   Sun,H., Xu,X., Chen,B., Wang,Y., Lyu,J., Guo,L., Yuan,Y. and Ren,D.
  TITLE     A novel intronic TCOF1 pathogenic variant in a Chinese family with
            Treacher Collins syndrome
  JOURNAL   BMC Med Genomics 17 (1), 75 (2024)
   PUBMED   38500116
  REMARK    GeneRIF: A novel intronic TCOF1 pathogenic variant in a Chinese
            family with Treacher Collins syndrome.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5026)
  AUTHORS   Valdez,B.C., Henning,D., So,R.B., Dixon,J. and Dixon,M.J.
  TITLE     The Treacher Collins syndrome (TCOF1) gene product is involved in
            ribosomal DNA gene transcription by interacting with upstream
            binding factor
  JOURNAL   Proc Natl Acad Sci U S A 101 (29), 10709-10714 (2004)
   PUBMED   15249688
  REMARK    GeneRIF: Results show that treacle is involved in ribosomal DNA
            gene transcription by interacting with upstream binding factor
            (UBF).
REFERENCE   5  (bases 1 to 5026)
  AUTHORS   Dixon,J., Edwards,S.J., Anderson,I., Brass,A., Scambler,P.J. and
            Dixon,M.J.
  TITLE     Identification of the complete coding sequence and genomic
            organization of the Treacher Collins syndrome gene
  JOURNAL   Genome Res 7 (3), 223-234 (1997)
   PUBMED   9074926
REFERENCE   6  (bases 1 to 5026)
  AUTHORS   Edwards,S.J., Gladwin,A.J. and Dixon,M.J.
  TITLE     The mutational spectrum in Treacher Collins syndrome reveals a
            predominance of mutations that create a premature-termination codon
  JOURNAL   Am J Hum Genet 60 (3), 515-524 (1997)
   PUBMED   9042910
REFERENCE   7  (bases 1 to 5026)
  AUTHORS   Dixon,M.J., Dixon,J., Houseal,T., Bhatt,M., Ward,D.C., Klinger,K.
            and Landes,G.M.
  TITLE     Narrowing the position of the Treacher Collins syndrome locus to a
            small interval between three new microsatellite markers at
            5q32-33.1
  JOURNAL   Am J Hum Genet 52 (5), 907-914 (1993)
   PUBMED   8488840
REFERENCE   8  (bases 1 to 5026)
  AUTHORS   Barbosa,M., Jabs,E.W. and Huston,S.
  TITLE     Treacher Collins Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301704
REFERENCE   9  (bases 1 to 5026)
  AUTHORS   Dixon,M.J., Dixon,J., Raskova,D., Le Beau,M.M., Williamson,R.,
            Klinger,K. and Landes,G.M.
  TITLE     Genetic and physical mapping of the Treacher Collins syndrome
            locus: refinement of the localization to chromosome 5q32-33.2
  JOURNAL   Hum Mol Genet 1 (4), 249-253 (1992)
   PUBMED   1303194
REFERENCE   10 (bases 1 to 5026)
  AUTHORS   Jabs,E.W., Li,X., Coss,C.A., Taylor,E.W., Meyers,D.A. and
            Weber,J.L.
  TITLE     Mapping the Treacher Collins syndrome locus to 5q31.3----q33.3
  JOURNAL   Genomics 11 (1), 193-198 (1991)
   PUBMED   1765376
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011372.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 19, 2019 this sequence version replaced XM_005268505.4.
            
            Summary: This gene encodes a nucleolar protein with a LIS1 homology
            domain. The protein is involved in ribosomal DNA gene transcription
            through its interaction with upstream binding factor (UBF).
            Mutations in this gene have been associated with Treacher Collins
            syndrome, a disorder which includes abnormal craniofacial
            development. Multiple transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Sep
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.4094401.1,
                                           SRR14038193.1326062.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000643257.2/ ENSP00000493815.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               AC011372.7         139329-139486
            159-214             AC011372.7         142788-142843
            215-354             AC011372.7         145745-145884
            355-428             AC011372.7         149476-149549
            429-615             AC011372.7         150348-150534
            616-689             AC011372.7         151161-151234
            690-920             AC011372.7         153638-153868
            921-1133            AC011372.7         155806-156018
            1134-1328           AC011372.7         156249-156443
            1329-1538           AC011372.7         156586-156795
            1539-1754           AC011372.7         156971-157186
            1755-1943           AC011372.7         157353-157541
            1944-2192           AC011372.7         157714-157962
            2193-2390           AC011372.7         158055-158252
            2391-2528           AC011372.7         160537-160674
            2529-2708           AC011372.7         160861-161040
            2709-2909           AC011372.7         161164-161364
            2910-3096           AC011372.7         169534-169720
            3097-3233           AC011372.7         171519-171655
            3234-3347           AC011372.7         173176-173289
            3348-3567           AC011372.7         173589-173808
            3568-3653           AC011372.7         174337-174422
            3654-3834           AC011372.7         175004-175184
            3835-4395           AC011372.7         177914-178474
            4396-4493           AC011372.7         179986-180083
            4494-4542           AC011372.7         180654-180702
            4543-5026           AC011372.7         181442-181925
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32-q33.1"
     gene            1..5026
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /note="treacle ribosome biogenesis factor 1"
                     /db_xref="GeneID:6949"
                     /db_xref="HGNC:HGNC:11654"
                     /db_xref="MIM:606847"
     exon            1..158
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..4520
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /note="isoform h is encoded by transcript variant 8;
                     Treacher Collins syndrome protein; nucleolar trafficking
                     phosphoprotein; Treacher Collins-Franceschetti syndrome 1"
                     /codon_start=1
                     /product="treacle protein isoform h"
                     /protein_id="NP_001358552.1"
                     /db_xref="CCDS:CCDS93805.1"
                     /db_xref="GeneID:6949"
                     /db_xref="HGNC:HGNC:11654"
                     /db_xref="MIM:606847"
                     /translation="MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPV
                     TLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKAT
                     PRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKS
                     AEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSV
                     KPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEG
                     SESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGK
                     PEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPG
                     KTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKG
                     AAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKP
                     ASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAP
                     AQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAP
                     AAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAV
                     AGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTA
                     PVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAAR
                     APSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVAT
                     AAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPT
                     PPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPA
                     ATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPR
                     IAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQ
                     PPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDS
                     EDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLL
                     SGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTG
                     GKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVL
                     TELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASV
                     SPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSN
                     PKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV"
     exon            159..214
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            215..354
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            355..428
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            429..615
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            616..689
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            690..920
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1133
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1328
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1538
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1754
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1943
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2192
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2390
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2528
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2708
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            2709..2909
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3096
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3233
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3347
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            3348..3567
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3653
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            3654..3834
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            3835..4395
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            4396..4493
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            4494..4542
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     exon            4543..5026
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5003..5008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /note="hexamer: AATAAA"
     polyA_site      5026
                     /gene="TCOF1"
                     /gene_synonym="MFD1; TCS; TCS1; treacle"
                     /note="major polyA site"
ORIGIN      
        1 ggggactaag gcggggcgtg caggtagccg gccggccggg ggtcgcgggt atggccgagg
       61 ccaggaagcg gcgggagcta cttcccctga tctaccacca tctgctgcgg gctggctatg
      121 tgcgtgcggc gcgggaagtg aaggagcaga gcggccagaa gtgtttcctg gctcagcccg
      181 taacccttct ggacatctat acacactggc aacaaacctc agagcttggt cggaagcgga
      241 aggcagagga agatgcggca ctgcaagcta agaaaacccg tgtgtcagac cccatcagca
      301 cctcggagag ctcggaagag gaggaagaag cagaagccga aaccgccaaa gccaccccaa
      361 gactagcatc taccaactcc tcagtcctgg gggcggactt gccatcaagc atgaaagaaa
      421 aagccaaggc agagacagag aaagctggca agactgggaa ttccatgcca caccctgcca
      481 ctgggaagac ggtggccaac cttctttctg ggaagtctcc caggaagtca gcagagccct
      541 cagcaaatac tacgttggtc tcagaaactg aggaggaggg cagcgtcccg gcctttggag
      601 ctgctgccaa gcctgggatg gtgtcagcgg gccaggccga cagctccagc gaggacacct
      661 ccagctccag tgatgagaca gacgtggagg ggaaaccctc agtaaaacca gcccaggtca
      721 aagcctcatc agtttctact aaggagtctc cagcaagaaa ggcggcccca gcccctggga
      781 aggtggggga tgtgacaccc caggtcaaag gaggggccct gcccccagcc aagagggcca
      841 agaagccaga agaggagtca gagagtagtg aggagggatc tgaaagtgag gaggaggccc
      901 ctgcagggac acgaagccag gtaaaggcct ctgaaaaaat tctccaggtc agagctgcct
      961 cagcccctgc caaggggacc cctgggaaag gggctacccc agcaccccct gggaaggcag
     1021 gggctgtagc ctcccagacc aaggcaggga agccagagga ggactcagag agcagcagcg
     1081 aggagtcatc tgacagtgag gaggagacgc cagctgccaa ggccctgctt caggcgaagg
     1141 cctcaggaaa aacctctcag gtcggagctg cctcagcccc tgccaaggag tcccccagga
     1201 aaggagctgc cccagcgccc cctgggaaga cagggcctgc agttgccaag gcccaggcgg
     1261 ggaagcggga ggaggactcg cagagcagca gcgaggaatc ggacagtgag gaggaggcgc
     1321 ctgctcaggc gaagccttca gggaaggccc cccaggtcag agccgcctcg gcccctgcca
     1381 aggagtcccc caggaaaggg gctgccccag cacctcctag gaaaacaggg cctgcagccg
     1441 cccaggtcca ggtggggaag caggaggagg actcaagaag cagcagcgag gagtcagaca
     1501 gtgacagaga ggcactggca gccatgaatg cagctcaggt gaagcccttg gggaaaagcc
     1561 cccaggtgaa acctgcctct accatgggca tggggccctt ggggaaaggc gccggcccag
     1621 tgccacccgg gaaggtgggg cctgcaaccc cctcagccca ggtggggaag tgggaggagg
     1681 actcagagag cagtagtgag gagtcatcag acagcagtga tggagaggtg cccacagctg
     1741 tggccccggc tcaggaaaag tccttgggga acatcctcca ggccaaaccc acctccagtc
     1801 ctgccaaggg gccccctcag aaggcagggc ctgtagccgt ccaggtcaag gctgaaaagc
     1861 ccatggacaa ctcggagagc agcgaggagt catcggacag tgcggacagt gaggaggcac
     1921 cagcagccat gactgcagct caggcaaaac cagctctgaa aattcctcag accaaggcct
     1981 gcccaaagaa aaccaatacc actgcatctg ccaaggtcgc ccctgtgcga gtgggcaccc
     2041 aagccccccg gaaagcagga actgcgactt ctccagcagg ctcatcccca gctgtggctg
     2101 ggggcaccca gagaccagca gaggattctt caagcagtga ggaatcagat agtgaggaag
     2161 agaagacagg tcttgcagta accgtgggac aggcaaagtc tgtggggaaa ggcctccagg
     2221 tgaaagcagc ctcagtgcct gtcaaggggt ccttggggca agggactgct ccagtactcc
     2281 ctgggaagac ggggcctaca gtcacccagg tgaaagctga aaagcaggaa gactctgaga
     2341 gcagtgagga ggaatcagac agtgaggaag cagctgcatc tccagcacag gtgaaaacct
     2401 cagtaaagaa aacccaggcc aaagccaacc cagctgccgc cagagcacct tcagcaaaag
     2461 ggacaatttc agcccctgga aaagttgtca ctgcagctgc tcaagccaag cagaggtctc
     2521 catccaaggt gaagccacca gtgagaaacc cccagaacag taccgtcttg gcgaggggcc
     2581 cagcatctgt gccatctgtg gggaaggccg tggctacagc agctcaggcc cagacagggc
     2641 cagaggagga ctcagggagc agtgaggagg agtcagacag tgaggaggag gcggagacgc
     2701 tggctcaggt gaagccttca gggaagaccc accagatcag agctgccttg gctcctgcca
     2761 aggagtcccc caggaaaggg gctgccccaa cacctcctgg gaagacaggg ccttcggctg
     2821 cccaggcagg gaagcaggat gactcaggga gcagcagcga ggaatcagac agtgatgggg
     2881 aggcaccggc agctgtgacc tctgcccagg tgattaaacc ccctctgatt tttgtcgacc
     2941 ctaatcgtag tccagctggc ccagctgcta cacccgcaca agcccaggct gcaagcaccc
     3001 cgaggaaggc ccgagcctcg gagagcacag ccaggagctc ctcctccgag agcgaggatg
     3061 aggacgtgat ccccgctaca cagtgcttga ctcctggcat cagaaccaat gtggtgacca
     3121 tgcccactgc ccacccaaga atagccccca aagccagcat ggctggggcc agcagcagca
     3181 aggagtccag tcggatatca gatggcaaga aacaggaggg accagccact caggtgtcaa
     3241 agaagaaccc agcttccctc ccactgaccc aggctgccct gaaggtcctc gcccagaaag
     3301 ccagtgaggc tcagcctcct gttgccagga cccagccttc aagtggggtt gacagtgctg
     3361 tgggaacact ccctgcaaca agtccccaga gcacctccgt ccaggccaaa gggaccaaca
     3421 agctcagaaa acctaagctt cctgaggtcc agcaggccac caaagcccct gagagctcag
     3481 atgacagtga ggacagcagc gacagttctt cagggagtga ggaagatggt gaagggcccc
     3541 agggggccaa gtcagcccac acgctggtag gtcccacccc ctccaggaca gagaccctgg
     3601 tggaggagac cgcagcagag tccagcgagg atgatgtggt ggcgccatcc cagtctctcc
     3661 tctcaggtta tatgacccct ggactaaccc cagccaattc ccaggcctca aaagccactc
     3721 ccaagctaga ctccagcccc tcagtttcct ctactctggc cgccaaagat gacccagatg
     3781 gcaagcagga ggcaaagccc caacaggcag caggcatgtt gtcccctaaa acaggtggaa
     3841 aagaggctgc ttcaggcacc acacctcaga agtcccggaa gcccaagaaa ggggctggga
     3901 acccccaagc ctcaaccctg gcgctgcaaa gcaacatcac ccagtgcctc ctgggccaac
     3961 cctggcccct gaatgaggcc caggtgcagg cctcagtggt gaaggtcctg actgagctgc
     4021 tggaacagga aagaaagaag gtggtggaca ccaccaagga gagcagcagg aagggctggg
     4081 agagccgcaa gcggaagcta tcgggagacc agccagctgc caggaccccc aggagcaaga
     4141 agaagaagaa gctgggggcc ggggaaggtg gggaggcctc tgtttcccca gaaaagacct
     4201 ccacgacttc caaggggaaa gcaaagagag acaaagcaag tggtgatgtc aaggagaaga
     4261 aagggaaggg gtctcttggc tcccaagggg ccaaggacga gccagaagag gagcttcaga
     4321 aggggatggg gacggttgaa ggtggagatc aaagcaaccc aaagagcaag aaggagaaga
     4381 agaaatccga caagagaaaa aaagacaaag aaaaaaaaga aaagaagaag aaagcaaaaa
     4441 aggcctcaac caaagattct gagtcaccgt cccagaagaa aaagaagaaa aagaagaaga
     4501 cagcagagca gactgtatga cgagcaccag caccaggcac agggatttcc tagccgagca
     4561 gtggccatcc ccatgcctct gacctccacc gacctctgcc caccatgggt tggaactaaa
     4621 ctgttacctt ccctcgctcc acagaagaag acagccagct tcaggggtcc ctgtgctggc
     4681 caagccagtg agcctgcggg gaggctggtc caaggagaaa gtggaccagc tcccatgacc
     4741 tcaccccact cccccaacac aggacgcttc atatagatgt gtacagtata tgtatttttt
     4801 taagtgacct cctctccttc cacagacccc acatgcccaa aggcctcggg acttcccacc
     4861 accttgctcc acagatccag ctaggcctga cctgtgcctc atcccgtgcc gctcggtctc
     4921 tggctgatcc cgaggctttg tcttcctctc gtcagttctt ttggttgtgt tttttgtttt
     4981 ttttttaata actcaaaaaa aaaataaaag acttggagga agggtg
//
