LOCUS       NM_006602               2625 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transcription factor like 5 (TCFL5), transcript
            variant 1, mRNA.
ACCESSION   NM_006602 XM_003960788
VERSION     NM_006602.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Lu,X., Zhang,Q. and Xie,Y.
  TITLE     TCFL5 knockdown sensitizes DLBCL to doxorubicin treatment via
            regulation of GPX4
  JOURNAL   Cell Signal 110, 110831 (2023)
   PUBMED   37516394
  REMARK    GeneRIF: TCFL5 knockdown sensitizes DLBCL to doxorubicin treatment
            via regulation of GPX4.
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Galan-Martinez,J., Stamatakis,K., Sanchez-Gomez,I.,
            Vazquez-Cuesta,S., Girones,N. and Fresno,M.
  TITLE     Isoform-specific effects of transcription factor TCFL5 on the
            pluripotency-related genes SOX2 and KLF4 in colorectal cancer
            development
  JOURNAL   Mol Oncol 16 (9), 1876-1890 (2022)
   PUBMED   34623757
  REMARK    GeneRIF: Isoform-specific effects of transcription factor TCFL5 on
            the pluripotency-related genes SOX2 and KLF4 in colorectal cancer
            development.
REFERENCE   3  (bases 1 to 2625)
  AUTHORS   Roth,L.M., Michal,M., Michal,M. Jr. and Cheng,L.
  TITLE     Protein expression of the transcription factors DMRT1, TCLF5, and
            OCT4 in selected germ cell neoplasms of the testis
  JOURNAL   Hum Pathol 82, 68-75 (2018)
   PUBMED   30067948
  REMARK    GeneRIF: Report differential expression of TCFL5 in normal human
            spermatogenesis, testicular mixed germ cell-sex cord stromal tumor,
            spermatocytic tumor, and seminoma.
REFERENCE   4  (bases 1 to 2625)
  AUTHORS   Yin,Y., Morgunova,E., Jolma,A., Kaasinen,E., Sahu,B.,
            Khund-Sayeed,S., Das,P.K., Kivioja,T., Dave,K., Zhong,F.,
            Nitta,K.R., Taipale,M., Popov,A., Ginno,P.A., Domcke,S., Yan,J.,
            Schubeler,D., Vinson,C. and Taipale,J.
  TITLE     Impact of cytosine methylation on DNA binding specificities of
            human transcription factors
  JOURNAL   Science 356 (6337) (2017)
   PUBMED   28473536
REFERENCE   5  (bases 1 to 2625)
  AUTHORS   Pattanshetty,S., Kotrashetti,V.S., Nayak,R., Bhat,K., Somannavar,P.
            and Babji,D.
  TITLE     PCR based detection of HPV 16 and 18 genotypes in normal oral
            mucosa of tobacco users and non-users
  JOURNAL   Biotech Histochem 89 (6), 433-439 (2014)
   PUBMED   24588599
  REMARK    GeneRIF: HPVs16 and 18 commonly are present in normal oral mucosa
            and emphasize the importance of distinguishing clinical
REFERENCE   6  (bases 1 to 2625)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   7  (bases 1 to 2625)
  AUTHORS   Rodriguez,C.I., Girones,N. and Fresno,M.
  TITLE     Cha, a basic helix-loop-helix transcription factor involved in the
            regulation of upstream stimulatory factor activity
  JOURNAL   J Biol Chem 278 (44), 43135-43145 (2003)
   PUBMED   12923186
  REMARK    GeneRIF: Cha is a new bHLH transcription factor that negatively
            regulates upstream transcription factor-dependent transcription
REFERENCE   8  (bases 1 to 2625)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (bases 1 to 2625)
  AUTHORS   Girones,N., Rodriguez,C.I., Carrasco-Marin,E., Hernaez,R.F., de
            Rego,J.L. and Fresno,M.
  TITLE     Dominant T- and B-cell epitopes in an autoantigen linked to Chagas'
            disease
  JOURNAL   J Clin Invest 107 (8), 985-993 (2001)
   PUBMED   11306602
REFERENCE   10 (bases 1 to 2625)
  AUTHORS   Maruyama,O., Nishimori,H., Katagiri,T., Miki,Y., Ueno,A. and
            Nakamura,Y.
  TITLE     Cloning of TCFL5 encoding a novel human basic helix-loop-helix
            motif protein that is specifically expressed in primary
            spermatocytes at the pachytene stage
  JOURNAL   Cytogenet Cell Genet 82 (1-2), 41-45 (1998)
   PUBMED   9763657
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL035669.43, BC046933.1, AB012124.1 and CA419503.1.
            
            On May 17, 2019 this sequence version replaced NM_006602.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB012124.1, AF070992.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335351.8/ ENSP00000334294.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AL035669.43        110941-110999       c
            60-376              BC046933.1         2-318
            377-2514            AB012124.1         179-2316
            2515-2616           CA419503.1         19-120              c
            2617-2625           AL035669.43        90182-90190         c
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     gene            1..2625
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /note="transcription factor like 5"
                     /db_xref="GeneID:10732"
                     /db_xref="HGNC:HGNC:11646"
                     /db_xref="MIM:604745"
     exon            1..799
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1655
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transcription factor-like 5 protein; cha transcription
                     factor; transcription factor-like 5 (basic
                     helix-loop-helix); HPV-16 E2-binding protein 1;
                     spermatogenesis- and oogenesis-specific bHLH-containing
                     protein 1"
                     /codon_start=1
                     /product="transcription factor-like 5 protein isoform 1"
                     /protein_id="NP_006593.2"
                     /db_xref="CCDS:CCDS13506.1"
                     /db_xref="GeneID:10732"
                     /db_xref="HGNC:HGNC:11646"
                     /db_xref="MIM:604745"
                     /translation="MSGPGPREPPPEAGAAGGEAAVEGAGGGDAALGEPGLSFTTTDL
                     SLVEMTEVEYTQLQHILCSHMEAAADGELETRLNSALLAAAGPGAGAGGFAAGGQGGA
                     APVYPVLCPSALAADAPCLGHIDFQELRMMLLSEAGAAEKTSGGGDGARARADGAAKE
                     GAGAAAAAAGPDGAPEARAKPAVRVRLEDRFNSIPAEPPPAPRGPEPPEPGGALNNLV
                     TLIRHPSELMNVPLQQQNKCTALVKNKTAATTTALQFTYPLFTTNACSTSGNSNLSQT
                     QSSSNSCSVLEAAKHQDIGLPRAFSFCYQQEIESTKQTLGSRNKVLPEQVWIKVGEAA
                     LCKQALKRNRSRMRQLDTNVERRALGEIQNVGEGATATQGAWQSSESSQANLGEQAQS
                     GPQGGRSQRRERHNRMERDRRRRIRICCDELNLLVPFCNAETDKATTLQWTTAFLKYI
                     QERHGDSLKKEFESVFCGKTGRRLKLTRPDSLVTCPAQGSLQSSPSMEIK"
     exon            800..983
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1146
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1390
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1532
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..2625
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2412..2417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /note="hexamer: AATAAA"
     polyA_site      2431
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
     regulatory      2493..2498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /note="hexamer: ATTAAA"
     polyA_site      2515
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /note="major polyA site"
     regulatory      2546..2551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
                     /note="hexamer: AATATA"
     polyA_site      2570
                     /gene="TCFL5"
                     /gene_synonym="bHLHe82; CHA; E2BP-1; Figlb; SOSF1"
ORIGIN      
        1 actcagcggt tttccctccc ccaacggcgc gccggctgtg gccggcgcag agtagtgctc
       61 gggccggggg tcgcctcccg cctcccgcct cccgcctccc gccgcccgcc gcccgcgtcg
      121 ccctcgccgc cgttgggccg cgccgcgccg ccatgtcggg ccccggaccg cgggagccgc
      181 cgccggaggc aggcgcggca ggcggcgagg cggccgtcga gggcgcgggc ggcggggacg
      241 cggcgctggg cgagccgggg ctgagcttca cgaccaccga cctgagcctg gtggagatga
      301 cggaggtgga gtacacgcag ctgcagcaca tcctctgctc gcacatggag gcggcggctg
      361 acggcgagct cgagacgcgc ctcaactcgg cgctgctggc ggcggcgggc ccgggcgcag
      421 gcgcgggcgg cttcgcggcg ggcggtcagg ggggcgcggc gcccgtgtac cccgtgctgt
      481 gcccgtccgc gctggcggcc gacgcgccct gcctgggcca catcgacttc caggagctgc
      541 gcatgatgct gctaagcgag gcgggcgcgg cggagaagac gtcgggcggc ggggacggag
      601 cgagggcccg ggccgacggc gccgccaagg agggcgcggg cgcggctgcg gctgcggctg
      661 gacccgacgg cgcgcccgag gcccgggcca agccggccgt gcgcgtccgc ctggaggacc
      721 gcttcaacag catccccgcc gagccgccgc ccgcgccgcg cggccccgag ccccccgagc
      781 cgggcggggc gctcaacaat ttggtaactc tcattcgaca tccatctgaa ctaatgaatg
      841 ttcctcttca gcaacaaaac aaatgtacag cattagtgaa aaataaaact gcggctacaa
      901 ctactgcttt gcaatttaca tacccactgt ttactacaaa tgcttgctct actagtggaa
      961 attctaatct ttcacagaca cagagttcta gtaactcatg ttctgtactt gaagctgcca
     1021 agcaccagga tattggattg cctagagcat tttctttctg ttatcagcaa gaaattgaat
     1081 ccactaaaca gacgttaggt agtagaaaca aagttttgcc tgagcaagtt tggattaaag
     1141 tgggagaagc agcgctatgc aaacaagcac tgaagaggaa tcggagtaga atgcgtcagt
     1201 tggacacaaa tgtagagcga agagcccttg gagagattca gaatgtgggc gaaggtgcca
     1261 ccgccacaca aggcgcttgg cagtcctcgg agtcctcaca ggcaaacctg ggggagcagg
     1321 cccagagtgg gccccaggga ggaaggtctc aacgtaggga gaggcataac cgaatggaaa
     1381 gagatagaag gcgcagaatc cgcatttgct gtgatgagtt gaatctctta gtgccgttct
     1441 gcaatgccga gactgacaag gccacaactc tgcagtggac cacagcattc ctgaaataca
     1501 tccaggaaag acatggagat tctcttaaaa aggaatttga gagcgtattt tgcggtaaaa
     1561 ctggccgaag gctaaagctg accagaccgg actccttggt gacctgtcct gcacagggga
     1621 gtttacagag cagcccctcg atggagatca agtgatcgga ctgaacagga atcctcgggg
     1681 ggtgaacagc cattccttcg tgacctgtgc acgccttctg caaccctgga gctctgctcg
     1741 gctagtctga ctcgaaaagg gcgtgactca agctgacggg actccagtag ggactttgag
     1801 agcacatttt gtaaaaatat ttatctagac gcaaatgctt atccatgaat gtcctcttag
     1861 accatttggg gatgaagcca tcttaataat tagtaataat taattagtaa taattagtaa
     1921 gcattttctc aatgctctga ttccatcatg ttttcttaac atgataactt aaaaaattga
     1981 catcctttgt actttcttta atcttaaaaa gtacacggct ttttacttat ttacctttta
     2041 aatatgcccc tttagcaatt ggaacaagtt aaattgttaa ctaaaaacag tttggaaatt
     2101 ttatttcatt cgttatatca cacccccttg tcatgactct gagtcacgtg ctgctgtatt
     2161 gcaacgtgca ggaccatttt aaacctgtgt gctaaaaatt ttccagatac ttgctttaaa
     2221 gctacttttg tccacaaatg aaatactgtc acagtagacg cttaaatgcc acgttttcat
     2281 accaagagtc attcattact tcatgtgtca caaactgtgg tgtttggaat tgggtttttc
     2341 aatgagtggc tttacttatc aatcacaaca ggtaatagca atagacgtta gtgcaataca
     2401 aagtcaccct caataaatac tgttaattgg agatgtgagt ttgtacacaa aacatcagac
     2461 tagacctttg tatgggagag aatttactgt acattaaatt ctttattttt tgttactctg
     2521 tagccagtca ttaatctgaa ggtttaatat atcattttat tgggatgaga tcatagtctt
     2581 tacacaaatg ctatgtaaac aagttactga atatttttca cctcg
//
