LOCUS       NM_001083962            8041 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens transcription factor 4 (TCF4), transcript variant 1,
            mRNA.
ACCESSION   NM_001083962
VERSION     NM_001083962.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,Y., Du,S., Wang,S., Liu,X., Cong,L., Shen,W. and Ye,K.
  TITLE     Circ_0004674 regulation of glycolysis and proliferation mechanism
            of osteosarcoma through miR-140-3p/TCF4 pathway
  JOURNAL   J Biochem Mol Toxicol 38 (9), e23846 (2024)
   PUBMED   39243204
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Honda,T., Nakagawa,T., Yuasa,T., Tokuda,Y., Nakano,M., Tashiro,K.,
            Tourtas,T., Schlotzer-Schrehardt,U., Kruse,F., Yamamoto,K.,
            Koizumi,N. and Okumura,N.
  TITLE     Dysregulation of the TCF4 Isoform in Corneal Endothelial Cells of
            Patients With Fuchs Endothelial Corneal Dystrophy
  JOURNAL   Invest Ophthalmol Vis Sci 65 (6), 27 (2024)
   PUBMED   38884552
  REMARK    GeneRIF: Dysregulation of the TCF4 Isoform in Corneal Endothelial
            Cells of Patients With Fuchs Endothelial Corneal Dystrophy.
REFERENCE   3  (bases 1 to 8041)
  AUTHORS   Bhattacharyya,N., Chai,N., Hafford-Tear,N.J., Sadan,A.N., Szabo,A.,
            Zarouchlioti,C., Jedlickova,J., Leung,S.K., Liao,T., Dudakova,L.,
            Skalicka,P., Parekh,M., Moghul,I., Jeffries,A.R., Cheetham,M.E.,
            Muthusamy,K., Hardcastle,A.J., Pontikos,N., Liskova,P., Tuft,S.J.
            and Davidson,A.E.
  TITLE     Deciphering novel TCF4-driven mechanisms underlying a common
            triplet repeat expansion-mediated disease
  JOURNAL   PLoS Genet 20 (5), e1011230 (2024)
   PUBMED   38713708
  REMARK    GeneRIF: Deciphering novel TCF4-driven mechanisms underlying a
            common triplet repeat expansion-mediated disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8041)
  AUTHORS   Wang,H., Chen,K., Zong,L., Zhao,X., Wang,J., Fan,S., Shen,B. and
            Zheng,S.
  TITLE     MALAT1/miR-7-5p/TCF4 Axis Regulating Menstrual Blood Mesenchymal
            Stem Cells Improve Thin Endometrium Fertility by the Wnt Signaling
            Pathway
  JOURNAL   Cell Transplant 33, 9636897241259552 (2024)
   PUBMED   38847385
  REMARK    GeneRIF: MALAT1/miR-7-5p/TCF4 Axis Regulating Menstrual Blood
            Mesenchymal Stem Cells Improve Thin Endometrium Fertility by the
            Wnt Signaling Pathway.
REFERENCE   5  (bases 1 to 8041)
  AUTHORS   Goldfarb,A.N., Lewandowska,K. and Shoham,M.
  TITLE     Determinants of helix-loop-helix dimerization affinity. Random
            mutational analysis of SCL/tal
  JOURNAL   J Biol Chem 271 (5), 2683-2688 (1996)
   PUBMED   8576241
REFERENCE   6  (bases 1 to 8041)
  AUTHORS   Sweetser,D.A., Elsharkawi,I., Yonker,L., Steeves,M., Parkin,K. and
            Thibert,R.
  TITLE     Pitt-Hopkins Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22934316
REFERENCE   7  (bases 1 to 8041)
  AUTHORS   Parisi,M.A.
  TITLE     Hirschsprung Disease Overview - RETIRED CHAPTER, FOR HISTORICAL
            REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301612
REFERENCE   8  (bases 1 to 8041)
  AUTHORS   Corneliussen,B., Thornell,A., Hallberg,B. and Grundstrom,T.
  TITLE     Helix-loop-helix transcriptional activators bind to a sequence in
            glucocorticoid response elements of retrovirus enhancers
  JOURNAL   J Virol 65 (11), 6084-6093 (1991)
   PUBMED   1681116
REFERENCE   9  (bases 1 to 8041)
  AUTHORS   Henthorn,P., McCarrick-Walmsley,R. and Kadesch,T.
  TITLE     Sequence of the cDNA encoding ITF-2, a positive-acting
            transcription factor
  JOURNAL   Nucleic Acids Res 18 (3), 678 (1990)
   PUBMED   2308860
REFERENCE   10 (bases 1 to 8041)
  AUTHORS   Henthorn,P., Kiledjian,M. and Kadesch,T.
  TITLE     Two distinct transcription factors that bind the immunoglobulin
            enhancer microE5/kappa 2 motif
  JOURNAL   Science 247 (4941), 467-470 (1990)
   PUBMED   2105528
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK122765.1, BC125084.1,
            BJ989626.1 and AC091103.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001083962.1.
            
            Summary: This gene encodes transcription factor 4, a basic
            helix-loop-helix transcription factor. The encoded protein
            recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a
            motif first identified in immunoglobulin enhancers. This gene is
            broadly expressed, and may play an important role in nervous system
            development. Defects in this gene are a cause of Pitt-Hopkins
            syndrome. In addition, an intronic CTG repeat normally numbering
            10-37 repeat units can expand to >50 repeat units and cause Fuchs
            endothelial corneal dystrophy. Multiple alternatively spliced
            transcript variants that encode different proteins have been
            described. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) differs in the 5' UTR and
            coding sequence compared to variant 3. The resulting isoform (a,
            also known as TCF4-B+) is shorter at the N-terminus compared to
            isoform c.
            
            Sequence Note: This gene is distinct from TCF7L2 (alias TCF-4).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.74931.1,
                                           SRR3476690.99891.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354452.8/ ENSP00000346440.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               AK122765.1         438-586
            150-2115            BC125084.1         1-1966
            2116-2510           BJ989626.1         1-395               c
            2511-7895           AC091103.4         87305-92689
            7896-8041           AC091103.4         92690-92835
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.2"
     gene            1..8041
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="transcription factor 4"
                     /db_xref="GeneID:6925"
                     /db_xref="HGNC:HGNC:11634"
                     /db_xref="MIM:602272"
     exon            1..155
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            156..247
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..2191
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="isoform a is encoded by transcript variant 1;
                     immunoglobulin transcription factor 2; SL3-3 enhancer
                     factor 2; class B basic helix-loop-helix protein 19"
                     /codon_start=1
                     /product="transcription factor 4 isoform a"
                     /protein_id="NP_001077431.1"
                     /db_xref="CCDS:CCDS42438.1"
                     /db_xref="GeneID:6925"
                     /db_xref="HGNC:HGNC:11634"
                     /db_xref="MIM:602272"
                     /translation="MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGH
                     FTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQS
                     KTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRP
                     LHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQ
                     DGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSS
                     LPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSP
                     DHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLER
                     LDDAIHVLRNHAVGPSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLM
                     VGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSS
                     GSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITRSRSSNNDDEDLTPEQKAERE
                     KERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQ
                     VRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDASNHMGQM"
     exon            248..320
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            321..382
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            383..479
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            480..544
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            545..674
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            675..724
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            725..830
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            831..964
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1097
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1165
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1244
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1321
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1525
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1661
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1824
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..2054
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2195
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..8041
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6752..6757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="hexamer: AATAAA"
     polyA_site      6774
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="major polyA site"
     regulatory      6894..6899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="hexamer: AACAAA"
     polyA_site      6922
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
     regulatory      7868..7873
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="hexamer: AATATA"
     polyA_site      7892
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
     regulatory      8020..8025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
                     /note="hexamer: ATTAAA"
     polyA_site      8041
                     /gene="TCF4"
                     /gene_synonym="bHLHb19; CDG2T; E2-2; FCD2; FECD3; ITF-2;
                     ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B;
                     SEF2-1D; TCF-4"
ORIGIN      
        1 gcggatctgt agtggcggcg gcggcggcgg cggcggggag gcagcaggcg cgggagcggg
       61 cgcaggagca ggcggcggcg gtggcggcgg cggttagaca tgaacgccgc ctcggcgccg
      121 gcggtgcacg gagagcccct tctcgcgcgc gggcggtttg tgtgattttg ctaaaatgca
      181 tcaccaacag cgaatggctg ccttagggac ggacaaagag ctgagtgatt tactggattt
      241 cagtgcgatg ttttcacctc ctgtgagcag tgggaaaaat ggaccaactt ctttggcaag
      301 tggacatttt actggctcaa atgtagaaga cagaagtagc tcagggtcct gggggaatgg
      361 aggacatcca agcccgtcca ggaactatgg agatgggact ccctatgacc acatgaccag
      421 cagggacctt gggtcacatg acaatctctc tccacctttt gtcaattcca gaatacaaag
      481 taaaacagaa aggggctcat actcatctta tgggagagaa tcaaacttac agggttgcca
      541 ccagcagagt ctccttggag gtgacatgga tatgggcaac ccaggaaccc tttcgcccac
      601 caaacctggt tcccagtact atcagtattc tagcaataat ccccgaagga ggcctcttca
      661 cagtagtgcc atggaggtac agacaaagaa agttcgaaaa gttcctccag gtttgccatc
      721 ttcagtctat gctccatcag caagcactgc cgactacaat agggactcgc caggctatcc
      781 ttcctccaaa ccagcaacca gcactttccc tagctccttc ttcatgcaag atggccatca
      841 cagcagtgac ccttggagct cctccagtgg gatgaatcag cctggctatg caggaatgtt
      901 gggcaactct tctcatattc cacagtccag cagctactgt agcctgcatc cacatgaacg
      961 tttgagctat ccatcacact cctcagcaga catcaattcc agtcttcctc cgatgtccac
     1021 tttccatcgt agtggtacaa accattacag cacctcttcc tgtacgcctc ctgccaacgg
     1081 gacagacagt ataatggcaa atagaggaag cggggcagcc ggcagctccc agactggaga
     1141 tgctctgggg aaagcacttg cttcgatcta ttctccagat cacactaaca acagcttttc
     1201 atcaaaccct tcaactcctg ttggctctcc tccatctctc tcagcaggca cagctgtttg
     1261 gtctagaaat ggaggacagg cctcatcgtc tcctaattat gaaggaccct tacactcttt
     1321 gcaaagccga attgaagatc gtttagaaag actggatgat gctattcatg ttctccggaa
     1381 ccatgcagtg ggcccatcca cagctatgcc tggtggtcat ggggacatgc atggaatcat
     1441 tggaccttct cataatggag ccatgggtgg tctgggctca gggtatggaa ccggccttct
     1501 ttcagccaac agacattcac tcatggtggg gacccatcgt gaagatggcg tggccctgag
     1561 aggcagccat tctcttctgc caaaccaggt tccggttcca cagcttcctg tccagtctgc
     1621 gacttcccct gacctgaacc caccccagga cccttacaga ggcatgccac caggactaca
     1681 ggggcagagt gtctcctctg gcagctctga gatcaaatcc gatgacgagg gtgatgagaa
     1741 cctgcaagac acgaaatctt cggaggacaa gaaattagat gacgacaaga aggatatcaa
     1801 atcaattact aggtcaagat ctagcaataa tgacgatgag gacctgacac cagagcagaa
     1861 ggcagagcgt gagaaggagc ggaggatggc caacaatgcc cgagagcgtc tgcgggtccg
     1921 tgacatcaac gaggctttca aagagctcgg ccgcatggtg cagctccacc tcaagagtga
     1981 caagccccag accaagctcc tgatcctcca ccaggcggtg gccgtcatcc tcagtctgga
     2041 gcagcaagtc cgagaaagga atctgaatcc gaaagctgcg tgtctgaaaa gaagggagga
     2101 agagaaggtg tcctcagagc ctccccctct ctccttggcc ggcccacacc ctggaatggg
     2161 agacgcatcg aatcacatgg gacagatgta aaagggtcca agttgccaca ttgcttcatt
     2221 aaaacaagag accacttcct taacagctgt attatcttaa acccacataa acacttctcc
     2281 ttaaccccca tttttgtaat ataagacaag tctgagtagt tatgaatcgc agacgcaaga
     2341 ggtttcagca ttcccaatta tcaaaaaaca gaaaaacaaa aaaaagaaag aaaaaagtgc
     2401 aacttgaggg acgactttct ttaacatatc attcagaatg tgcaaagcag tatgtacagg
     2461 ctgagacaca gcccagagac tgaacggcaa tctttccaca ctgtggaaca atgcatttgt
     2521 gcctaaactt cttttggaaa aaaaaaatat aattaatttg taagtctgaa aaaaaaatat
     2581 ttaatttaaa aaaaattgta aacttgcaat aatgaaaaag tgtacttctg aagaaaacta
     2641 catgaacgtt tttgttggta ttcaagtcag ctagtgttta taattactgg atattgaatt
     2701 aggggaagct cggctgccct agtaacaaaa ccagcaaacg tcctgatgac aacgaagtga
     2761 tgacattagc cattccttag ggtaggagga acagatggat cttatagacc tatgacaaat
     2821 atatatataa atatatatat aaatatatat taaaaattta gtgactatgg taagcttttg
     2881 ttcatttgtt tcagactttt ttctcctgta aaaaaatagt actgattaac ttttttaaaa
     2941 gaaagatttt actgtaaata tggatttttt tttttttggt cttatttctg tccctttccc
     3001 tggtttgtta tcgtaacctg tagtgccaac tctgcttcca gaggggtagt gcaggatgaa
     3061 atgctgaccc tgatgttgct tctcattcat aaataagtag aaagttgttt ctccagtctt
     3121 ttgggaacac aggacttaaa agtcacatca tgtgtagata ttacaagcag cattaccaag
     3181 acatggcaaa aagagtttgt ctgaattgta atgttgcgtt tgtgaaccta ttctgggatt
     3241 ttcagaggta caaggttaga atgctacaat gttaccactg tgccttccaa tgtttatatc
     3301 atcggaaaca taacataatc aaagtggctg tgatttaaca aaatgattaa agtgttacct
     3361 acctgtgtag ccgaagtagt gtgcagtgag gcgtttctga atacatggtc agatttttgg
     3421 aaaaaaacaa aaacaaaaaa aacaagtaaa gttcaaaaac cgtcaaatga gaaaattgca
     3481 agtagtgtga cagagctgat tgattttgtt gctttcttga tttttttttt caaaatgggt
     3541 ttactaaaat gtagatgact taactgcctc ctccttcgtc tgaaaaatgc caatattcaa
     3601 tcatcatgca gcattataac aagccttata agtcctaaag cattaagttg cacttttttg
     3661 aggaggggta gtgcagtatt tctctggcca gtatgaatga agtttatact taccatattt
     3721 gatagaaaca tagatcaagc tatggcacag cgactcatca gatagctagc tttgacgtct
     3781 gggcacaatt gaaccaactt ccatcgtgaa tctttataat gattgacttt ggtgtatagt
     3841 gcagtaaaca aatagtgctc ctagttaagt atttgtcagc atccttttgt ctctaacttg
     3901 tttctatttt tacagccaca caattcttgg catgtattaa gaaaaaaaaa aatccctgtt
     3961 caagtagttt ttccacctat cagcactgag taaatgccat aaatccattg aaatggtcta
     4021 aatgttccat ctgttctcct gttttgccag ttatatagta atgaaataca tttgtaaatt
     4081 ttatgcaaca aatggcaaac gtatcattat tttgaaattg tgtatgtaaa agttatattt
     4141 ttacatgtag actcttgtta ttatgtgttt taatacattg tatcagtttt tgtttttttt
     4201 taaaaactgt ggtttaaaaa gaagtctcat ttaaatgaaa tagctacaag aatcagaatt
     4261 ttatgttcat ttctgaaaat gtaagaacaa ataagatagt taccacgtgg tcatctttta
     4321 caaacccata aacattttga ttagctgtgt gtgtgttgaa aaactgtaaa tatgttcagt
     4381 agcgataaaa ctaaaataac tttgatttgt tgataagttc ctaaaatgtg gaggtggatt
     4441 aaaaccttag gagaatagca gaaatcaaac ttcatgaaaa gttattttgg ggctttcctg
     4501 tgaaatgtat gaacaaagag gctcagagaa ggacatggaa gacaataatg tatactctct
     4561 cctcctccct gaataatgaa aaccatgtgt atttgttccc tccgtatgtt aaagatttcc
     4621 ttttagtggt acattctgca ctcattttgt atagtctacc aaggcgggta tccctaggaa
     4681 caatattata taggaagcag gtatactctg atcacattca ggataagtgt acagaagaaa
     4741 atacggtgtt tactctttag ggaactggaa acactccctg cattgatgta cattttaaga
     4801 atggcacttt tgatacatgt tatcataaag gtgcttaata gagctgaatt aaagtttttc
     4861 aaatctgtaa acaaagcaaa aaagtaaatt gtagtcattt gattattttt taaattggtg
     4921 ctttatattt tgttctcact cagagtaaaa gctgcaattt attgttcacc agctttgatg
     4981 tattcattac tcagtaatgc aatacctcta ttgttgaatt ccctttggaa ataagtgaaa
     5041 attctaacgg ccactgaaag ctgctcgcta ggttttgctt ggtggagaaa cataatctgc
     5101 acctatccat attaattggg ttgtatcccc attaaaaaag aaaaaaaggg aatgtggcct
     5161 ttttagtgtg ttttttattg ttgttgtttt gtaattatca aacccaggta agatattggt
     5221 atcctgcact ggattttcaa atgaagttca gcagaagaca gttaagatta aagtactata
     5281 caaaaatttc aaaagggtcc atactacgct atctgtatga cgacacttag gctggggatc
     5341 tctttcagaa actcggactt taaaagcaac ttggagcagt tgatccacct ccacattcaa
     5401 gtaatttatg aatatgcaga atagggatct gttcatctag aaatttttac catttgtctt
     5461 ctgtgtagct gcaaggaaca ctaatgttta tacaactgtc agtccaccca gtggtgcaac
     5521 tggttctgat tcagtcttcc gattcctttt tatttttcac tttttcctat ttctgaattt
     5581 ttttttttat ttgtgatctt gattttgatg aggggttggg gagtggggag ggagtcgaac
     5641 caagacttgg agttaagagg attttcatct tttgcatcca acaggcagaa tatgatctgt
     5701 gtccaaaagt gaacttgagt caggaatgaa tcaatttcag cataaacaag cacaaaaatt
     5761 tagtctgctg gctgactgga agcaaaaaag tcaagatgga atatgatgaa ttccaacaca
     5821 atggggcacc aaggccttta ggcctctctt tttattttgc tttggttttg tttgtttttc
     5881 tttagagaca tgctctttct catgggactt gaagtggact catctttgtg cagtgctggt
     5941 tttgccatac tcatttcaag tattatagac atatgtaatg gtgaaaatat atgaactgtg
     6001 gcctttttca ttcttgttac ttgtgatgca attaagtgaa gataagaaaa aaaaaaaaaa
     6061 agcagagatt taccatgtat cagtgcctgg ctttttgtta taaagctttg tttgtctagt
     6121 gctcttttgc tataaaatag actgtagtac accctagtag gaaaaaaaaa aaactaaatt
     6181 taaaaataaa aaatatattt ggcttatttt tcgcaggagc aatcctttta taccatgaat
     6241 attacaaaaa aattgtcaga ttctgaatat ttcttctttg tagatttttg gaatcattat
     6301 gagtaaaagt ttgttacttt attttactat ttaaaagatg ttattttacc atgtgttacc
     6361 aagatgaaac tgtatgggta gcttttttgt ttgttttttg ttttgttttt gtttttgttt
     6421 ttgtttttag ttgtaggtcg cagcggggaa attttttgcg actgtacaca tagctgcagc
     6481 attaaaaact taaaaaaatt gttaaaaaaa aaaaaaggga aaacatttca aaaaaaaaaa
     6541 aaaagataaa cagttacacc ttgttttcaa tgtgtggctg agtgcctcga ttttttcatg
     6601 tttttggtgt atttctgatt tgtagaagtg tccaaacagg ttgtgtgctg gagttccttc
     6661 aagacaaaaa caaacccagc ttggtcaagg ccattacctg tttcccatct gtagttattc
     6721 gatgaagtca tgtacatgac cgttctgtag caataaatgt gccattttta taaactgttt
     6781 ctgacacttg tttcatttca ttttgcattg tccatatagc tatgattctc ttctgtaagt
     6841 aaaacgcatc tatatttcat tttccaagtg ttggaggtat tgacagctta acaaacaaaa
     6901 catacaaaaa aaatcacaaa aacaaattga aaagcaaagc acatgattga tcaaggaaga
     6961 gatgccctta atgaaaatgg aacgggatgc atgcaaaaca aaaagaaaac tgtctagagg
     7021 attaactaat tgaaggaata taattaatgt gtgtgtaaca ctgaagctat gcatttgaag
     7081 agctctgaac tgcaccagtg ttttcggttg tgctgcaggt tgctaagtca agtcagcctt
     7141 aaccttttgc accagttggt cggctgtttg gcagaacatt ctcagatctt ttcagtcaaa
     7201 aatctaagat gatttatttt gtatcacttt gttaaaagct gaatattgtt aactacagtt
     7261 aatattaaca ctgtatttat actttctcaa actacatccg ccccaccact tctggttgcc
     7321 tctgttgact attaatccag atgtaaacaa ccagatgttt ttttctaact tgtacaaact
     7381 gacgtgtgtc aactatcatg gaaggaaaaa aatgtacaga ttaaaattat tcagtgttat
     7441 gtactgtaag ttaatatttt tgtagaatgg acatcaatct actttgcaaa atttggaggc
     7501 tatttcaaca ttgcactgta gaaatgtaaa gtaatgtatg caatgtaaag gaaagcccgc
     7561 ggtagctgag cgcttcataa cagaatgttc taatcaagta cgtggtattt ggggatgtct
     7621 ccaatattgc tcttgtattc tttctaattg ggtttagtga ctagttgaag gaaaatgtta
     7681 taacgccatt tggttcacat gtgaagtgcc ctccatagcc aaatgttggg attttttttt
     7741 ttttcgtttt tggttggact gtttgcagat atttaaattt tatgaaattt ccaaagattt
     7801 tggttgataa ccccctttta ccttctaaat gatttgagat gttcttatgt tcttactgtg
     7861 tgttttaaat atatataaaa gagccacaag catttagtct tttagtatta tatttgatca
     7921 tataactact actgaaaagg ggtggggggg aatgtgcctg tgcaccccta cagtatcttg
     7981 tgtagtgtct attaacattg ttctctttta gaggaccaca ttaaaaggta cattaatctt
     8041 a
//
