LOCUS       NM_001378418            7624 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens transcription factor 20 (TCF20), transcript variant 3,
            mRNA.
ACCESSION   NM_001378418 XM_006724313
VERSION     NM_001378418.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Zhang,W., Wan,P., Zhang,M., Chang,Y., Du,S., Jin,T. and Wang,Y.
  TITLE     Association Between CYP2D7 and TCF20 Polymorphisms and Coronary
            Heart Disease
  JOURNAL   Cardiovasc Toxicol 24 (10), 1037-1046 (2024)
   PUBMED   39060884
  REMARK    GeneRIF: Association Between CYP2D7 and TCF20 Polymorphisms and
            Coronary Heart Disease.
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Schneeweiss,M.R., Dale,B. and Ejaz,R.
  TITLE     Diagnosis and clinical presentation of two individuals with a rare
            TCF20 pathogenic variant
  JOURNAL   BMJ Case Rep 15 (12), e248995 (2022)
   PUBMED   36593604
  REMARK    GeneRIF: Diagnosis and clinical presentation of two individuals
            with a rare TCF20 pathogenic variant.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7624)
  AUTHORS   Levy,J., Cogan,G., Maruani,A., Maillard,A., Dupont,C., Drunat,S.,
            Rachid,M., Atzori,P., Delorme,R., Jeyarajah,S., Isidor,B.,
            Pichon,O., Moradkhani,K., Verloes,A. and Tabet,A.C.
  TITLE     Rare and de novo duplications containing TCF20 are associated with
            a neurodevelopmental disorder
  JOURNAL   Clin Genet 101 (3), 364-370 (2022)
   PUBMED   34904221
  REMARK    GeneRIF: Rare and de novo duplications containing TCF20 are
            associated with a neurodevelopmental disorder.
REFERENCE   4  (bases 1 to 7624)
  AUTHORS   Torti,E., Keren,B., Palmer,E.E., Zhu,Z., Afenjar,A., Anderson,I.J.,
            Andrews,M.V., Atkinson,C., Au,M., Berry,S.A., Bowling,K.M.,
            Boyle,J., Buratti,J., Cathey,S.S., Charles,P., Cogne,B.,
            Courtin,T., Escobar,L.F., Finley,S.L., Graham,J.M. Jr.,
            Grange,D.K., Heron,D., Hewson,S., Hiatt,S.M., Hibbs,K.A.,
            Jayakar,P., Kalsner,L., Larcher,L., Lesca,G., Mark,P.R., Miller,K.,
            Nava,C., Nizon,M., Pai,G.S., Pappas,J., Parsons,G., Payne,K.,
            Putoux,A., Rabin,R., Sabatier,I., Shinawi,M., Shur,N.,
            Skinner,S.A., Valence,S., Warren,H., Whalen,S., Crunk,A.,
            Douglas,G., Monaghan,K.G., Person,R.E., Willaert,R., Solomon,B.D.
            and Juusola,J.
  TITLE     Variants in TCF20 in neurodevelopmental disability: description of
            27 new patients and review of literature
  JOURNAL   Genet Med 21 (9), 2036-2042 (2019)
   PUBMED   30739909
  REMARK    GeneRIF: We provide valuable data regarding the prognosis and
            clinical manifestations of patients with variants in TCF20.
REFERENCE   5  (bases 1 to 7624)
  AUTHORS   Vetrini,F., McKee,S., Rosenfeld,J.A., Suri,M., Lewis,A.M.,
            Nugent,K.M., Roeder,E., Littlejohn,R.O., Holder,S., Zhu,W.,
            Alaimo,J.T., Graham,B., Harris,J.M., Gibson,J.B., Pastore,M.,
            McBride,K.L., Komara,M., Al-Gazali,L., Al Shamsi,A., Fanning,E.A.,
            Wierenga,K.J., Scott,D.A., Ben-Neriah,Z., Meiner,V., Cassuto,H.,
            Elpeleg,O., Holder,J.L. Jr., Burrage,L.C., Seaver,L.H., Van
            Maldergem,L., Mahida,S., Soul,J.S., Marlatt,M., Matyakhina,L.,
            Vogt,J., Gold,J.A., Park,S.M., Varghese,V., Lampe,A.K., Kumar,A.,
            Lees,M., Holder-Espinasse,M., McConnell,V., Bernhard,B., Blair,E.,
            Harrison,V., Muzny,D.M., Gibbs,R.A., Elsea,S.H., Posey,J.E., Bi,W.,
            Lalani,S., Xia,F., Yang,Y., Eng,C.M., Lupski,J.R. and Liu,P.
  CONSRTM   DDD study
  TITLE     De novo and inherited TCF20 pathogenic variants are associated with
            intellectual disability, dysmorphic features, hypotonia, and
            neurological impairments with similarities to Smith-Magenis
            syndrome
  JOURNAL   Genome Med 11 (1), 12 (2019)
   PUBMED   30819258
  REMARK    GeneRIF: TCF20 pathogenic variants are associated with a novel
            syndrome manifesting clinical characteristics similar to those
            observed in Smith-Magenis syndrome.
            Erratum:[Genome Med. 2019 Mar 25;11(1):16. doi:
            10.1186/s13073-019-0630-1.. PMID: 30909959]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7624)
  AUTHORS   Babbs,C., Lloyd,D., Pagnamenta,A.T., Twigg,S.R., Green,J.,
            McGowan,S.J., Mirza,G., Naples,R., Sharma,V.P., Volpi,E.V.,
            Buckle,V.J., Wall,S.A., Knight,S.J., Parr,J.R. and Wilkie,A.O.
  CONSRTM   International Molecular Genetic Study of Autism Consortium (IMGSAC)
  TITLE     De novo and rare inherited mutations implicate the transcriptional
            coregulator TCF20/SPBP in autism spectrum disorder
  JOURNAL   J Med Genet 51 (11), 737-747 (2014)
   PUBMED   25228304
  REMARK    GeneRIF: mutations in TCF20 are associated with Autism spectrum
            disorders
REFERENCE   7  (bases 1 to 7624)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
REFERENCE   8  (bases 1 to 7624)
  AUTHORS   Rajadhyaksha,A., Riviere,M., Van Vooren,P., Szpirer,J., Szpirer,C.,
            Babin,J. and Bina,M.
  TITLE     Assignment of AR1, transcription factor 20 (TCF20), to human
            chromosome 22q13.3 with somatic cell hybrids and in situ
            hybridization
  JOURNAL   Cytogenet Cell Genet 81 (3-4), 176-177 (1998)
   PUBMED   9730594
REFERENCE   9  (bases 1 to 7624)
  AUTHORS   Kirstein,M., Sanz,L., Quinones,S., Moscat,J., Diaz-Meco,M.T. and
            Saus,J.
  TITLE     Cross-talk between different enhancer elements during mitogenic
            induction of the human stromelysin-1 gene
  JOURNAL   J Biol Chem 271 (30), 18231-18236 (1996)
   PUBMED   8663478
REFERENCE   10 (bases 1 to 7624)
  AUTHORS   Sanz,L., Moscat,J. and Diaz-Meco,M.T.
  TITLE     Molecular characterization of a novel transcription factor that
            controls stromelysin expression
  JOURNAL   Mol Cell Biol 15 (6), 3164-3170 (1995)
   PUBMED   7760812
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z83851.17 and BX247885.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 12, 2020 this sequence version replaced XM_006724313.3.
            
            Summary: This gene encodes a transcription factor that recognizes
            the platelet-derived growth factor-responsive element in the matrix
            metalloproteinase 3 promoter. The encoded protein is thought to be
            a transcriptional coactivator, enhancing the activity of
            transcription factors such as JUN and SP1. Mutations in this gene
            are associated with autism spectrum disorders. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Sep
            2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.1575113.1,
                                           SRR14038196.3487127.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000677622.1/ ENSP00000503828.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-315               Z83851.17          17876-18190         c
            316-6006            BX247885.11        89139-94829         c
            6007-6100           BX247885.11        59097-59190         c
            6101-6150           BX247885.11        49335-49384         c
            6151-6278           BX247885.11        48097-48224         c
            6279-7624           BX247885.11        39501-40846         c
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.2"
     gene            1..7624
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /note="transcription factor 20"
                     /db_xref="GeneID:6942"
                     /db_xref="HGNC:HGNC:11631"
                     /db_xref="MIM:603107"
     exon            1..315
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /inference="alignment:Splign:2.1.0"
     exon            316..6006
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /inference="alignment:Splign:2.1.0"
     CDS             352..6234
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /note="isoform 1 is encoded by transcript variant 3;
                     stromelysin-1 platelet-derived growth factor-responsive
                     element binding protein; SPRE-binding protein; nuclear
                     factor SPBP; stromelysin-1 PDGF-responsive element-binding
                     protein"
                     /codon_start=1
                     /product="transcription factor 20 isoform 1"
                     /protein_id="NP_001365347.1"
                     /db_xref="CCDS:CCDS14033.1"
                     /db_xref="GeneID:6942"
                     /db_xref="HGNC:HGNC:11631"
                     /db_xref="MIM:603107"
                     /translation="MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGT
                     GGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTG
                     TPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPF
                     SPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLP
                     SSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHN
                     VGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNA
                     ATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQT
                     GENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTD
                     PGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPE
                     EQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQN
                     EPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQD
                     KGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQ
                     SGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHG
                     ERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNEL
                     ASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEP
                     GGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGL
                     VSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATH
                     DSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDP
                     HHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQ
                     MYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYG
                     PPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPP
                     GMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFIS
                     PIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDL
                     PSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGD
                     TVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEE
                     WRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDS
                     KNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKK
                     QQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEP
                     EIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQR
                     SLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYP
                     QDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHP
                     RFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQI
                     PELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCY
                     NKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPLPPLQNKTAKGSLSTEQSE
                     RG"
     exon            6007..6100
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /inference="alignment:Splign:2.1.0"
     exon            6101..6150
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /inference="alignment:Splign:2.1.0"
     exon            6151..6278
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /inference="alignment:Splign:2.1.0"
     exon            6279..7624
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6943..6948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /note="hexamer: AATAAA"
     polyA_site      6968
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
     regulatory      7603..7608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /note="hexamer: AATAAA"
     polyA_site      7624
                     /gene="TCF20"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /note="major polyA site"
ORIGIN      
        1 gttatttcct gtgtgttggg agaatggaga gaaaaaaccc cgagcccagc cgctttcggc
       61 ccctgcgagg cggccgcggc ctccgccagg ggagcccgcc ctgaggcgcg cctgaccggg
      121 gagcgagcgc gcgggccgga gcgagcctcg aggggccgcg gccccggccc gccccgccgc
      181 cggcccgggc gcccgagctg gaggcggcgg agccaccccc acccccaccc cgcgcagcgc
      241 cgcccgccgt cgccaccgct ccctccccgc ccgcccccgg cggaggccga gggactgggc
      301 ggggggctcc tgcaggaggg ctgttggcct gctgctgtgc tgctgaacag tatgcagtcc
      361 tttcgggagc aaagcagtta ccacggaaac cagcaaagct acccacagga ggtacacggc
      421 tcatcccggc tagaagagtt cagccctcgt caggcccaga tgttccagaa ttttggaggt
      481 acaggtggca gtagtggcag cagtggcagt ggcagtggtg gtggacgacg aggagcagca
      541 gctgctgcgg cagcgatggc tagcgagacc tctggccatc aaggttacca gggtttcagg
      601 aaagaggctg gagattttta ctacatggca ggcaacaaag accccgtgac tacaggaacc
      661 ccacagcctc ctcagcgaag gccttctggg cctgtgcaga gctatggacc cccccagggg
      721 agcagctttg gcaatcagta tgggagtgag ggtcatgtgg gccagtttca agcacagcac
      781 tctggccttg gcggtgtgtc acattatcag caggattaca ctgggccttt ctctccaggg
      841 agtgctcagt accaacagca ggcttccagc cagcagcagc agcagcaagt ccagcagttg
      901 agacaacagc tttaccagtc ccatcagccc ctgccacagg ccactggcca accagcatcc
      961 agctcatccc atctacagcc aatgcagcgg ccctcaactc tgccatcctc tgctgctggt
     1021 taccagttaa gagtgggtca gtttggccaa cactatcagt cttctgcttc ctcctcctcc
     1081 tcctcctcct tcccttcacc acagcgtttt agccagtctg gacagagcta tgatggcagt
     1141 tacaatgtga atgctggatc tcagtatgaa ggacacaatg tgggttctaa tgcacaggct
     1201 tatggaacac aatccaatta cagctatcag cctcaatcta tgaagaattt tgaacaggca
     1261 aagattccac aagggaccca acaggggcag cagcagcagc aaccgcagca acaacaacac
     1321 ccttctcagc atgtgatgca gtatactaac gctgccacca agctgcccct gcaaagccaa
     1381 gtggggcagt acaaccagcc tgaggttcct gtgaggtccc ccatgcagtt tcaccagaac
     1441 ttcagcccca tttctaaccc ttctccagct gcctctgtgg ttcagtctcc aagctgtagt
     1501 tctaccccat ctcctctcat gcagactggg gagaatctcc agtgtgggca aggcagtgtg
     1561 cctatgggtt ccagaaacag aattttacag ttaatgcctc aactcagtcc aaccccatca
     1621 atgatgccca gtcctaattc tcatgctgca ggcttcaaag ggtttggact agaaggggta
     1681 ccagaaaagc gactgacaga tcctgggttg agtagtttga gtgctctgag tactcaagtg
     1741 gccaatcttc ctaacactgt ccagcacatg ttactttctg atgccctgac tcctcagaag
     1801 aagacctcca agaggccctc atcttccaag aaagcagata gctgcacaaa ttctgaaggc
     1861 tcctcacaac ctgaagaaca gctgaagtcc cctatggcag agtcattaga tggaggctgc
     1921 tccagcagtt cagaggatca aggcgagaga gtgcggcaac taagtggcca gagcaccagc
     1981 tctgacacca cctacaaggg tggagcctct gagaaagctg gctcctcacc ggcacaaggt
     2041 gctcagaatg aaccccccag actcaatgct agtcctgccg caagagaaga ggccacctca
     2101 ccaggcgcta aggacatgcc attgtcatcc gacgggaacc caaaggttaa tgagaagact
     2161 gttggggtga ttgtctcccg ggaagccatg acaggtcggg tagaaaagcc tggtggacaa
     2221 gataaaggct cccaagagga tgatcctgca gccactcaaa ggccacctag caatggtggg
     2281 gcaaaggaaa ccagtcatgc atcacttccc cagccagagc ctccaggagg aggagggagc
     2341 aaaggaaaca agaatggcga taacaactcc aaccataatg gagaaggaaa tggccagagt
     2401 ggccactctg cagcgggccc tggttttacg agcagaactg agcctagcaa atctcctgga
     2461 agtctgcgct atagttacaa agatagtttc gggtcagccg tgccacgaaa tgtcagtggc
     2521 tttcctcagt atcctacagg gcaagaaaag ggagatttca ctggccatgg ggaacgaaag
     2581 ggtagaaatg aaaaattccc aagcctcctg caggaagtgc ttcagggtta ccaccaccac
     2641 cctgacagga gatattctag gagtactcaa gagcatcagg ggatggctgg tagcctagaa
     2701 ggaaccacaa ggcccaatgt cttggttagt caaaccaatg aattagctag caggggcctt
     2761 ctgaacaaaa gcattgggtc tctattagaa aatccccact ggggcccctg ggaaaggaaa
     2821 tcaagcagca cagctcctga aatgaaacag atcaatttga ctgactatcc aattcccaga
     2881 aagtttgaaa tagagcctca gtcatcagca catgagcctg ggggttccct ctctgaaaga
     2941 agatcagtga tctgtgatat ttctccacta agacagattg tcagggaccc aggggctcac
     3001 tcactgggac acatgagtgc cgacaccaga attgggagga atgaccgtct caatccaact
     3061 ttaagtcagt cggtcattct tcctggtggt ttggtgtcca tggaaaccaa gctgaaatcc
     3121 cagagcgggc agataaaaga ggaagacttt gaacagtcta aatctcaagc tagtttcaac
     3181 aacaagaaat ctggagacca ctgccatcct cctagcatca agcatgagtc ttaccgcggc
     3241 aatgccagcc ctggagcagc aacccatgat tccctttcag actatggccc gcaagacagc
     3301 agacccacgc caatgcggcg ggtccctggc agagttggtg gtcgggaggg catgaggggt
     3361 cggtcccctt ctcaatatca tgactttgca gaaaaattga aaatgtctcc tgggcggagc
     3421 agaggcccag ggggagaccc tcatcacatg aatccacaca tgaccttttc agagagggct
     3481 aaccggagtt ctttacacac tcccttttct cccaactcag aaaccctggc ctctgcttat
     3541 catgcaaata ctcgggctca tgcttatggg gaccctaacg caggtttgaa ttctcagctg
     3601 cattataaga gacagatgta ccaacagcaa ccagaggagt ataaagactg gagcagcggt
     3661 tctgctcagg gagtaattgc tgcagcacag cacaggcagg aggggccacg gaagagtcca
     3721 aggcagcagc agtttcttga cagagtacgg agccctctga aaaatgacaa agatggtatg
     3781 atgtatggcc caccagtggg gacttaccat gaccccagtg cccaggaggc tgggcgctgc
     3841 ctaatgtcta gtgatggtct gcctaacaag ggcatggaat taaagcatgg ctcccagaag
     3901 ttacaagaat cctgttggga tctttctcgg caaacttctc cagccaaaag cagcggtcct
     3961 ccaggaatgt ccagtcaaaa aaggtatggg ccgccccatg agactgatgg acatggacta
     4021 gctgaggcta cacagtcatc caaacctggt agtgttatgc tgagacttcc aggccaggag
     4081 gatcattctt ctcaaaaccc cttaatcatg aggaggcgtg ttcgttcttt tatctctccc
     4141 attcccagta agagacagtc acaagatgta aagaacagta gcactgaaga taaaggtcgc
     4201 ctccttcact catcaaaaga aggcgctgat aaagcattca attcctatgc ccatctttct
     4261 cacagtcagg atatcaagtc tatccctaag agagattcct ccaaggacct tccaagtcca
     4321 gatagtagaa actgccctgc tgttaccctc acaagccctg ctaagaccaa aatactgccc
     4381 ccacggaaag gacggggatt gaaattggaa gctatagttc agaagattac atccccaaat
     4441 attaggagga gcgcatcttc gaacagtgcg gaggctgggg gagacacggt tacgcttgat
     4501 gatatactgt ctttgaagag tggtcctcct gaaggtggga gtgttgctgt tcaggatgct
     4561 gacatagaga agagaaaagg tgaggtggct tcggacctag tcagtccagc aaaccaggag
     4621 ttgcacgtag agaaacctct tccaaggtct tcagaagagt ggcgtggcag cgtggatgac
     4681 aaagtgaaga cagagacaca tgcagaaaca gttactgccg gaaaggaacc ccctggtgcc
     4741 atgacatcca caacctcaca gaagcctggt agtaaccaag ggagaccaga tggttccctg
     4801 ggtggaacag cacctttaat ctttccagac tcaaagaatg tacctccagt gggcatattg
     4861 gcccctgagg caaaccccaa ggctgaagag aaggagaacg atacagtgac gatttcaccg
     4921 aagcaagagg gtttccctcc aaagggatat ttcccatcag gaaagaagaa ggggagaccc
     4981 attggtagtg tgaataagca aaagaaacag cagcagccac cgcctccacc ccctcagccc
     5041 ccacagatac cagaaggttc tgcagatgga gagccaaagc caaaaaaaca gaggcaaagg
     5101 agggagagaa ggaagcctgg ggcccagccg aggaagcgaa aaaccaaaca agcagttccc
     5161 attgtggaac cccaagaacc tgagatcaaa ctaaaatatg ccacccagcc actggataaa
     5221 actgatgcca agaacaagtc tttttaccct tacatccatg tagtaaataa gtgtgaactt
     5281 ggagccgttt gtacaatcat caatgctgag gaagaagaac agaccaaatt agtgaggggc
     5341 aggaagggtc agaggtcact gacccctcca cctagcagca ctgaaagcaa ggcgctcccg
     5401 gcctcgtcct ttatgctgca gggacctgtt gtgacagagt cttcggttat ggggcacctg
     5461 gtttgctgtc tgtgtggcaa gtgggccagt taccggaaca tgggtgacct ctttggacct
     5521 ttttatcccc aagattatgc agccactctc ccgaagaatc cacctcctaa gagggccaca
     5581 gaaatgcaga gcaaagttaa ggtacggcac aaaagtgctt ctaatggctc caagacggac
     5641 actgaggagg aggaagagca gcagcagcag cagaaggagc agagaagcct ggccgcacac
     5701 cccaggttta agcggcgcca ccgctcggaa gactgtggtg gaggccctcg gtccctgtcc
     5761 agggggctcc cttgtaaaaa agcagccact gagggcagca gtgaaaagac tgttttggac
     5821 tcgaagccct ccgtgcccac cacttcagaa ggtggccctg agctggagtt acaaatccct
     5881 gaactacctc ttgacagcaa tgaattttgg gtccatgagg gttgtattct ctgggccaat
     5941 ggaatctacc tggtttgtgg caggctctat ggcctgcagg aagcgctgga aatagccaga
     6001 gagatgaaat gttcccactg ccaggaggca ggcgccacct tgggctgcta caacaaaggc
     6061 tgctccttcc gataccatta cccgtgtgcc attgatgcag attgtttgct acatgaggag
     6121 aacttctcgg tgaggtgccc taagcacaag cctccccttc cgtgccctct cccccccttg
     6181 cagaacaaga ccgcgaaagg cagcctcagc acagagcagt cggagcgggg gtgagggggg
     6241 cagtgtgctc gtgggaatgg aaaggacagc aagcacaggt gagactgtgg agatgagaag
     6301 gtggtggaca ctcgtgatgg aatggaaatc gtcctaccgt gcagccacac cctgccctgc
     6361 cccgccccgc cccgcccgcg tgcctgccca tgccagcact tccttaagtt ctcacatcac
     6421 actcaaacca gtgacaccac aggaaagaaa gacccaagac gttggaatgg ctgtttccat
     6481 ggacacaatc tccatagtga caatgtgggg ggagggggga ggggtgggat gatggggaaa
     6541 gggtgggggg aattaaaagg gagggataaa tatatatata taaatctatt tttagtctgg
     6601 aaagactttg tttaaatgaa aggtgcgcta tcccttttga ttctgtttta aaattatctc
     6661 gttaaagatc tccaaatttg ttccgatgac aagtgaaatt taaatgtgag attgaactga
     6721 acaaaccctc atctcatgaa ggacggggtg tgtgtgtggc gttgatcttt agcctgtctc
     6781 acaccagttc agaaaacact agacccagga ttgaaaaagc aaaccacagc agaaccatcc
     6841 ttttgtcatt aatttgtctc aaagtgggaa ggttttgggg gagggggaaa tacagggatg
     6901 gtccatgttt tcaagagtag gggaatgatg tttaaacaca aaaataaatt ttttttcatt
     6961 tccagaaaca ctatttattt atggtttttt ttttttaatt ttttcttttt gggggtgaaa
     7021 ttggcagatg cctgaggtca tagctgtgtc ctgggtcact gtggctggtg aggacctcaa
     7081 ggaccccatc aagtgtacac agcagcagca aaatcaaggg atgaccctcc tctggggccc
     7141 cctgtcctca gcacattcca ggcagctgtg ccctgaccca cagggacccg tggggatggg
     7201 aggaggtcca ggcctgtgtt gccagagctg gcagtgtgag ctgtaggcag ggacggggag
     7261 ggactgtcgc tgtgatcaga gtgggttaag ctgaccagga acacccattt aacccctttt
     7321 tctttttgct ttcattttta taaaggaaaa gaggacctgt cagataggca gccccatgct
     7381 acgtgattct ttatgttgtg ttgttttgtt ttgtaaattg tataattttt aaatatctga
     7441 gttttaaaaa aagaaaaaag tacaaaaaaa tcttgttatg gccttaagaa ggggttagtg
     7501 catctttcag gggtcactct gccatgggga taaaatagct gtttcacaaa cagttttatt
     7561 taaaaaaaca aaaaacaaaa aaaatcaaaa aatcaaaaaa ataataaact tcattttaac
     7621 cttg
//
