LOCUS       NM_001163435            7961 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens TBC1 domain containing kinase (TBCK), transcript
            variant 1, mRNA.
ACCESSION   NM_001163435
VERSION     NM_001163435.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Chand,P., Sulaiman,A. and Kirmani,S.
  TITLE     Mutation Of TBC1 Domain Containing Kinase (TBCK) With Associated
            Intellectual Disability And Hypotonia
  JOURNAL   J Pak Med Assoc 73 (10), 2083-2085 (2023)
   PUBMED   37876076
  REMARK    GeneRIF: Mutation Of TBC1 Domain Containing Kinase (TBCK) With
            Associated Intellectual Disability And Hypotonia.
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Durham,E.L., Angireddy,R., Black,A., Melendez-Perez,A., Smith,S.,
            Gonzalez,E.M., Navarro,K.G., Diaz,A., Bhoj,E.J.K. and Katsura,K.A.
  TITLE     TBCK syndrome: a rare multi-organ neurodegenerative disease
  JOURNAL   Trends Mol Med 29 (10), 783-785 (2023)
   PUBMED   37455236
  REMARK    GeneRIF: TBCK syndrome: a rare multi-organ neurodegenerative
            disease.
REFERENCE   3  (bases 1 to 7961)
  AUTHORS   Nair,D., Diaz-Rosado,A., Varella-Branco,E., Ramos,I., Black,A.,
            Angireddy,R., Park,J., Murali,S., Yoon,A., Ciesielski,B.,
            O'Brien,W.T., Passos-Bueno,M.R. and Bhoj,E.
  TITLE     Heterozygous variants in TBCK cause a mild neurologic syndrome in
            humans and mice
  JOURNAL   Am J Med Genet A 191 (10), 2508-2517 (2023)
   PUBMED   37353954
  REMARK    GeneRIF: Heterozygous variants in TBCK cause a mild neurologic
            syndrome in humans and mice.
REFERENCE   4  (bases 1 to 7961)
  AUTHORS   Quentin,D., Schuhmacher,J.S., Klink,B.U., Lauer,J., Shaikh,T.R.,
            Huis In 't Veld,P.J., Welp,L.M., Urlaub,H., Zerial,M. and
            Raunser,S.
  TITLE     Structural basis of mRNA binding by the human FERRY Rab5 effector
            complex
  JOURNAL   Mol Cell 83 (11), 1856-1871 (2023)
   PUBMED   37267906
REFERENCE   5  (bases 1 to 7961)
  AUTHORS   Wu,J., Li,Q., Li,Y., Lin,J., Yang,D., Zhu,G., Wang,L., He,D., Lu,G.
            and Zeng,C.
  TITLE     A long type of TBCK is a novel cytoplasmic and mitotic
            apparatus-associated protein likely suppressing cell proliferation
  JOURNAL   J Genet Genomics 41 (2), 69-72 (2014)
   PUBMED   24576458
  REMARK    GeneRIF: localization and function of TBCK
REFERENCE   6  (bases 1 to 7961)
  AUTHORS   Liu,Y., Yan,X. and Zhou,T.
  TITLE     TBCK influences cell proliferation, cell size and mTOR signaling
            pathway
  JOURNAL   PLoS One 8 (8), e71349 (2013)
   PUBMED   23977024
  REMARK    GeneRIF: TBCK may play an important role in cell proliferation,
            cell growth and actin organization possibly by modulating mTOR
            pathway.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7961)
  AUTHORS   Wilk,J.B., Shrine,N.R., Loehr,L.R., Zhao,J.H., Manichaikul,A.,
            Lopez,L.M., Smith,A.V., Heckbert,S.R., Smolonska,J., Tang,W.,
            Loth,D.W., Curjuric,I., Hui,J., Cho,M.H., Latourelle,J.C.,
            Henry,A.P., Aldrich,M., Bakke,P., Beaty,T.H., Bentley,A.R.,
            Borecki,I.B., Brusselle,G.G., Burkart,K.M., Chen,T.H., Couper,D.,
            Crapo,J.D., Davies,G., Dupuis,J., Franceschini,N., Gulsvik,A.,
            Hancock,D.B., Harris,T.B., Hofman,A., Imboden,M., James,A.L.,
            Khaw,K.T., Lahousse,L., Launer,L.J., Litonjua,A., Liu,Y.,
            Lohman,K.K., Lomas,D.A., Lumley,T., Marciante,K.D., McArdle,W.L.,
            Meibohm,B., Morrison,A.C., Musk,A.W., Myers,R.H., North,K.E.,
            Postma,D.S., Psaty,B.M., Rich,S.S., Rivadeneira,F., Rochat,T.,
            Rotter,J.I., Soler Artigas,M., Starr,J.M., Uitterlinden,A.G.,
            Wareham,N.J., Wijmenga,C., Zanen,P., Province,M.A., Silverman,E.K.,
            Deary,I.J., Palmer,L.J., Cassano,P.A., Gudnason,V., Barr,R.G.,
            Loos,R.J., Strachan,D.P., London,S.J., Boezen,H.M.,
            Probst-Hensch,N., Gharib,S.A., Hall,I.P., O'Connor,G.T., Tobin,M.D.
            and Stricker,B.H.
  TITLE     Genome-wide association studies identify CHRNA5/3 and HTR4 in the
            development of airflow obstruction
  JOURNAL   Am J Respir Crit Care Med 186 (7), 622-632 (2012)
   PUBMED   22837378
REFERENCE   8  (bases 1 to 7961)
  AUTHORS   Fukuda,M.
  TITLE     TBC proteins: GAPs for mammalian small GTPase Rab?
  JOURNAL   Biosci Rep 31 (3), 159-168 (2011)
   PUBMED   21250943
  REMARK    Review article
REFERENCE   9  (bases 1 to 7961)
  AUTHORS   Kalsi,G., Kuo,P.H., Aliev,F., Alexander,J., McMichael,O.,
            Patterson,D.G., Walsh,D., Zhao,Z., Schuckit,M., Nurnberger,J. Jr.,
            Edenberg,H., Kramer,J., Hesselbrock,V., Tischfield,J.A.,
            Vladimirov,V., Prescott,C.A., Dick,D.M., Kendler,K.S. and
            Riley,B.P.
  TITLE     A systematic gene-based screen of chr4q22-q32 identifies
            association of a novel susceptibility gene, DKK2, with the
            quantitative trait of alcohol dependence symptom counts
  JOURNAL   Hum Mol Genet 19 (12), 2497-2506 (2010)
   PUBMED   20332099
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Hum Mol Genet. 2010 Oct 15;19(20):4121. Hesselbrock,
            Victor [added]; Tischfield, Jay A [added]]
REFERENCE   10 (bases 1 to 7961)
  AUTHORS   Ishibashi,K., Kanno,E., Itoh,T. and Fukuda,M.
  TITLE     Identification and characterization of a novel Tre-2/Bub2/Cdc16
            (TBC) protein that possesses Rab3A-GAP activity
  JOURNAL   Genes Cells 14 (1), 41-52 (2009)
   PUBMED   19077034
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093680.2, AC114734.4,
            AC107381.2 and AC109361.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001163435.2.
            
            Summary: This gene encodes a protein that contains a protein kinase
            domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC)
            domain. The encoded protein is thought to play a role in actin
            organization, cell growth and cell proliferation by regulating the
            mammalian target of the rapamycin (mTOR) signaling pathway. This
            protein may also be involved in the transcriptional regulation of
            the components of the mTOR complex. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Mar 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1 and 2 encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK315571.1, SRR14372079.592382.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394708.7/ ENSP00000378198.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-279               AC093680.2         33998-34276         c
            280-501             AC093680.2         26835-27056         c
            502-574             AC093680.2         13161-13233         c
            575-689             AC114734.4         69564-69678         c
            690-763             AC114734.4         67903-67976         c
            764-905             AC114734.4         59332-59473         c
            906-966             AC114734.4         57884-57944         c
            967-1028            AC114734.4         56387-56448         c
            1029-1090           AC114734.4         55711-55772         c
            1091-1239           AC114734.4         54605-54753         c
            1240-1378           AC114734.4         52092-52230         c
            1379-1478           AC114734.4         49936-50035         c
            1479-1528           AC114734.4         44225-44274         c
            1529-1658           AC114734.4         43856-43985         c
            1659-1757           AC114734.4         42735-42833         c
            1758-1820           AC114734.4         41054-41116         c
            1821-1947           AC114734.4         40404-40530         c
            1948-1998           AC114734.4         39195-39245         c
            1999-2082           AC114734.4         37829-37912         c
            2083-2168           AC114734.4         20216-20301         c
            2169-2205           AC114734.4         2184-2220           c
            2206-2367           AC114734.4         1075-1236           c
            2368-2543           AC107381.2         99605-99780         c
            2544-2719           AC107381.2         44713-44888         c
            2720-2879           AC107381.2         23992-24151         c
            2880-7961           AC109361.4         119273-124354       c
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q24"
     gene            1..7961
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /note="TBC1 domain containing kinase"
                     /db_xref="GeneID:93627"
                     /db_xref="HGNC:HGNC:28261"
                     /db_xref="MIM:616899"
     exon            1..279
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            280..501
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..2990
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /note="isoform a is encoded by transcript variant 1; TBC
                     domain-containing protein kinase-like protein; FERRY
                     endosomal RAB5 effector complex subunit 1"
                     /codon_start=1
                     /product="TBC domain-containing protein kinase-like
                     protein isoform a"
                     /protein_id="NP_001156907.2"
                     /db_xref="CCDS:CCDS54788.1"
                     /db_xref="GeneID:93627"
                     /db_xref="HGNC:HGNC:28261"
                     /db_xref="MIM:616899"
                     /translation="MFPLKDAEMGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQI
                     LKTITHPRLCQYVDISRGKHERLVVVAEHCERSLEDLLRERKPVSCSTVLCIAFEVLQ
                     GLQYMNKHGIVHRALSPHNILLDRKGHIKLAKFGLYHMTAHGDDVDFPIGYPSYLAPE
                     VIAQGIFKTTDHMPSKKPLPSGPKSDVWSLGIILFELCVGRKLFQSLDISERLKFLLT
                     LDCVDDTLIVLAEEHGCLDIIKELPETVIDLLNKCLTFHPSKRPTPDQLMKDKVFSEV
                     SPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDINNDYLAERSIEEVYYLWCLAGG
                     DLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRL
                     KDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLK
                     AYPYKKNQIWKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVD
                     IPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALA
                     YACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAI
                     PWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLF
                     SDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPYFSAECPDP
                     PKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVD
                     IRNSEDFIRGHISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTA
                     EFAAHLVKMKYPRICILDGGINKIKPTGLLTIPSPQI"
     exon            502..574
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            575..689
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            690..763
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            764..905
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            906..966
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1028
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1090
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1239
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1378
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1478
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1528
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1658
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1757
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1820
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1947
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..1998
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2082
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2168
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2205
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..2367
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2543
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2719
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2879
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..7961
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3445..3450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /note="hexamer: AATAAA"
     polyA_site      3480
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /note="major polyA site"
     regulatory      5221..5226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /note="hexamer: ATTAAA"
     polyA_site      5243
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
     regulatory      7943..7948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
                     /note="hexamer: AATAAA"
     polyA_site      7961
                     /gene="TBCK"
                     /gene_synonym="FERRY1; Fy-1; HSPC302; IHPRF3; TBCKL"
ORIGIN      
        1 agagggtgaa aagctcggat ctgtgtttgg ggaaggccag gcttgcgctc ctcgccgggt
       61 tccgcgaagg ttaaccttgg ctgacttggc tcgcgagcaa agggcagcgt ctgagctccc
      121 ggcgttccag gagtggcctc ttttgtagga gcacctgaaa tgcagcgtct ggtgcactaa
      181 gccgtagcgg cagcagcagc cacagcgaca gcgctggggc cctgtgtaga agctccatcc
      241 cccttgtctt tgtgcttgcc tgcgtcccca gactcagaga ttatcttaga agacctagga
      301 ctccaaaaat gtttcccctg aaggacgctg aaatgggagc ctttaccttc tttgcctcgg
      361 ctctgccaca tgatgtttgt ggaagcaatg gacttcctct cacaccaaat tccatcaaaa
      421 ttttagggcg ctttcaaatc cttaaaacca tcacccatcc cagactctgc cagtatgtgg
      481 atatttctag gggaaagcat gaacgactag tggtcgtggc tgaacattgt gaacgtagtc
      541 tggaagactt gcttcgagaa aggaaacctg tgagctgttc aacggttttg tgtatagcat
      601 ttgaggttct tcagggcttg cagtatatga acaaacatgg tatagtacac agggcattgt
      661 ctcctcataa tatcctgttg gaccgaaagg gacatattaa attggctaaa tttggacttt
      721 atcacatgac agctcatggt gatgatgttg atttcccaat agggtatccc tcgtacttgg
      781 cccctgaggt aattgcacag ggaattttca aaaccactga tcacatgcca agtaaaaaac
      841 cattgccttc tggccccaaa tcagatgtat ggtctcttgg aatcatttta tttgagcttt
      901 gtgtgggaag aaaattattt cagagcttgg atatttctga aagactaaaa tttttgctta
      961 ctttggattg tgtagatgac actttaatag ttctggctga agagcatggt tgtttggaca
     1021 ttataaagga gcttcctgaa actgtgatag atcttttgaa taagtgcctt accttccatc
     1081 cttctaagag gccaacccca gatcaattaa tgaaggacaa agtattcagt gaggtatcac
     1141 ctttatatac cccctttacc aaacctgcca gtctgttttc atcttctctg agatgtgctg
     1201 atttaactct gcctgaggat atcagtcagt tgtgtaaaga tataaataat gattacctgg
     1261 cagaaagatc tattgaagaa gtgtattacc tttggtgttt ggctggaggt gacttggaga
     1321 aagagcttgt caacaaggaa atcattcgat ccaaaccacc tatctgcaca ctccccaatt
     1381 ttctctttga ggatggtgaa agctttggac aaggtcgaga tagaagctcg cttttagatg
     1441 ataccactgt gacattgtcg ttatgccagc taagaaatag attgaaagat gttggtggag
     1501 aagcatttta cccattactt gaagatgacc agtctaattt acctcattca aacagcaata
     1561 atgagttgtc tgcagctgcc acgctccctt taatcatcag agagaaggat acagagtacc
     1621 aactaaatag aattattctc ttcgacaggc tgctaaaggc ttatccatat aaaaaaaacc
     1681 aaatctggaa agaagcaaga gttgacattc ctcctcttat gagaggttta acctgggctg
     1741 ctcttctggg agttgaggga gctattcatg ccaagtacga tgcaattgat aaagacactc
     1801 caattcctac agatagacaa attgaagtgg atattcctcg ctgtcatcag tacgatgaac
     1861 tgttatcatc accagaaggt catgcaaaat ttaggcgtgt attaaaagcc tgggtagtgt
     1921 ctcatcctga tcttgtgtat tggcaaggtc ttgactcact ttgtgctcca ttcctatatc
     1981 taaacttcaa taatgaagcc ttggcttatg catgtatgtc tgcttttatt cccaaatacc
     2041 tgtataactt cttcttaaaa gacaactcac atgtaataca agagtatctg actgtcttct
     2101 ctcagatgat tgcatttcat gatccagagc tgagtaatca tctcaatgag attggtttca
     2161 ttccagatct ctatgccatc ccttggtttc ttaccatgtt tactcatgta tttccactac
     2221 acaaaatttt ccacctctgg gataccttac tacttgggaa ttcctctttc ccattctgta
     2281 ttggagtagc aattcttcag cagctgcggg accggctttt ggctaatggc tttaatgagt
     2341 gtattcttct cttctccgat ttaccagaaa ttgacattga acgctgtgtg agagaatcta
     2401 tcaacctgtt ttgttggact cctaaaagtg ctacttacag acagcatgct caacctccaa
     2461 agccatcttc tgacagcagt ggaggcagaa gttcggcacc ttatttctct gctgagtgtc
     2521 cagatcctcc aaagacagat ctgtcaagag aatccatccc attaaatgac ctgaagtcag
     2581 aagtatcacc acggatttca gcagaggacc tgattgactt gtgtgagctc acagtgacag
     2641 gccacttcaa aacacccagc aagaaaacaa agtccagtaa accaaagctc ctggtggttg
     2701 acatccggaa tagtgaagac tttattcgtg gtcacatttc aggaagcatc aacattccat
     2761 tcagtgctgc cttcactgca gaaggggagc ttacccaggg cccttacact gctatgctcc
     2821 agaacttcaa agggaaggtc attgtcatcg tggggcatgt ggcaaaacac acagctgagt
     2881 ttgcagctca ccttgtgaag atgaaatatc caagaatctg tattctagat ggtggcatta
     2941 ataaaataaa gccaacaggc ctcctcacca tcccatctcc tcaaatatga agaaccaaga
     3001 gtgtgactgc caaaacttag tgtggcatca gcaccaacag cacagttctt catatccacg
     3061 ccactctcag acaaaactag atgtccagat tgttgcattt ccgtaaagtt tgtcacgaga
     3121 cattttttaa aatctcataa cccacatgtt cagttatcca tgcaagaaac ttgactctac
     3181 atgtattgct gaaagaattt tcttaacagt gaaatctgat catatatttt taccacactg
     3241 ccacataaag cccaagaaat tcagctgaca agacagattt agcattatca agaaatccca
     3301 tttgccctga aaaagctgtc ctccattgta ctgaacagac agtcctgtcg attgtgttat
     3361 ttagaaacat acactgaatg tgggctgaaa tcatcatctt tccataatga aaactgagaa
     3421 actattcaca atgcattcct tataaataaa tgctacattt agtaactcat ttcacccaaa
     3481 caagggggat gtgtgtgtgt gtgtgtgtat aggaagtgga gtttatccca ttgcagaaag
     3541 tggtaatact tactcccaga aaaatgaaat ttagaaacca tttatatttg atagaatatt
     3601 tggtcagttc ctgtagcaaa gacgaatggc ttaaacaaat tttctagttt ctttatcaca
     3661 tgaaagtctg tacagtcagt ccagggctag tctactggtt tcctgatcat taagaactca
     3721 ttaccttctc tcattgcttt acaaacctca atatgtggca tccatctcat ggatgaaaat
     3781 ggctcctcag cttctaccat cacatctgct atctagaagg aagagaatga gggaaggagg
     3841 gaggggatga agagaaaaga aggaacaaaa gaaggaggga ggaaagaaga aaagaaagga
     3901 aaagaagcga agcacactcc ttccctttaa ggtcataaac cagaagttgc gcacactacc
     3961 tctgcttaca tatcattggc cagaacttgg gtgcatggcc acacctagct ttaaggaaaa
     4021 ctggaaatag agtctttggg tggttgatca tttccccagc taaaaacatt cttattactg
     4081 tgtgggaaga agagagtaca tattgaggta cagctaacaa cctctgcctc tgccacattg
     4141 tttttaaaat gagtattata tttcattaaa tctaagacat ctcacaattt taggtaccac
     4201 aaagaaaaaa tgaagccaaa tgaattgtga cactttgtca attaaaagac acattccctg
     4261 ggaggccgag gtcaggagtt tgagaccagc ctggccaaca tggggaaact ttgtctctac
     4321 taaaaatata aaaattagct ggtcatagtg gcacacgtct gcggtcccag ctactcagga
     4381 ggctgaggca ggagaatcac ttgaacctga gaggcagagg ttgcaatgag ccatgatagg
     4441 gccactgtac cccagcctgg gcaacagggc tcaccctgcc atctcaaaaa aaaaaaaaaa
     4501 aagaaagata cattcccatt tcagaaatct gaaaaaaaaa atgcctcata tatatgaaat
     4561 atagcatatc ctttaattat cttcaatttt gttttaacta aaaagtgttc attcatctat
     4621 aacttacatt ccactccaat gttaagactg cctaggcaga acatttcaat aagagcctca
     4681 attcagcttt ctgggccatg gttttgttgt tgctgctatt gtttacttta ttttattttt
     4741 tctttgcatc tactttttaa attgagatat acttaaccat aaaattcatc cttttaacat
     4801 gtacagttca gtagttttta gtgtattcac aaagttgtgc aatcagtatt atccagaata
     4861 ctttcattgt atgcaattgt ctacctgatc agcaggtatt ccacattaac ccctcctaag
     4921 gtctttgata aatttttgta tatggagtga ggggtccagg ttcattcttg catatgagta
     4981 tccagttgtc ccaggacccc tcctgttttg tttgttttat tcaatttatt tggtcattca
     5041 ccaaatatgt tttgaatacc tactatgtat ttctaagaca ctaatataat ccatagttat
     5101 atcatttgtg atccttacct agtttacaag gaaaaaaatg tgagaattaa tgagaacaaa
     5161 tatataccaa gatttcacta acattgtttt tgggccattg tctaccctct tcagagaaat
     5221 attaaatcct cctgtcctgg caattactat tttcttcctt cagtctttga tgacttacct
     5281 gagttcataa tcagaatgca ccattgatga tcattctgag tcttaactct gccttactga
     5341 gaaagcagag aagagaggga tggaggggca gtgatttgag ttgaataagg tggtaggtag
     5401 taggagagga tgaagacctt gtgaagagag ctatgtaaga agataatagt agcaaccctc
     5461 tgtcatgatt ttgcatttga tctttcttaa aacgacacat ttaggcttat tccaagaaat
     5521 tgaagttttt taaacatgaa aattataata tttaatattc tcaacagtaa agtaagagca
     5581 cactatcact gccatgttta tacacagggc atgttatttc atggtggatg atacgaacag
     5641 ttgacctcaa caactatttg ttagattaaa cgtatataac ccaggattta tttgagttcc
     5701 tttttaatgt attattgatt cctacacgat gactatcagc actgtttctg gctttacctg
     5761 gaatgccata agtaccacca ccagcccaca cagatataca tacacacaca cacacacaca
     5821 cactcacatt cataaacaca cttattaact cttgataatc tagcaacaac agtgtacctc
     5881 ctccagtctt ctctggcttc cccatgctgc actaagagcc ccaaatctac taaagcattc
     5941 cattcataat ttactaataa tagatttatt acatcatttt gcaatcgttt gtttacattt
     6001 agtcccctat taaaatatga gctgcttaag atcaggagct ataatttata cctcttgcta
     6061 ccttcagcaa cccaccacat tggatatgct caattaatat aaaattgaat ctatcaattt
     6121 tagaatctat ttgaagggag aaaaataaaa ataacctgtg gtaatttttt tcaactgcca
     6181 aacttatctg gtacctgata gtatagaaat aaattgaaat ctcaattcat ggagtgaaaa
     6241 aaggatctct aaagtcccat ccagctctaa aatctgataa ttctagaagt tggagcagat
     6301 aacttccaaa ataaggtaat ttaatctagt tgcatcctct tttcaaaata cagaaatgtt
     6361 agattcaaaa agcatattaa aaataactaa tgcttaacca agtttgaaag aaagaagttt
     6421 tttgatgtca gaaatgaaaa ggacctgaaa ggcacagctt ttaggcccgt gggataccca
     6481 gtatcaatat aagccccaag aactcaggtt ttggaataca tataggaata gaagacatag
     6541 ctttggatct tatgggtggg agttctaatt aagacctctt tatgaagtga ccgtctcaag
     6601 gaactccgct ccatgaaatg acaagacata acttcccgcc aaattaggga agctacagag
     6661 gagattgtca ttggcctggt tattcttgat gaaggaagaa aaatctgtta cagacagagt
     6721 tgttcagtgg aaagtctggg gtaagttcct gatttagaac aggctttagt gcactttaaa
     6781 aggaaaacaa tgaattatat aaaactttta gaggacacag tggtgccaga tttttgttgg
     6841 attcctgtgc tttcctcctc caaatagtta ccaaaaaata ttcctttggg aattagtatc
     6901 aaaaggattc tatagcatct caaaagaatc ttattcactt tgggaggccg aggcaggcag
     6961 atcacaaggt caggagatcg agaccatcct ggctaacacg gtgaaacccc atctctacta
     7021 aaaatacaaa aaattagcca ggcgtggtgg tgggcgcctg tagtcccagc tactcgggag
     7081 gctgaggcag gagaatggcg tgaacccagg aggcagagct tgcagtgagc cgagatcgct
     7141 ccactgcact ccagcctggg agacagagtg agactccgtc tcaaaaaaga aaaaaaaaaa
     7201 tcttatgcag tagtcttatt taataaaatc cactacaagg agagaagact gctgccttga
     7261 ctatatagaa agggttggca gatatttcat tctatcagtt gtaactagta atcccattgt
     7321 tatacatgct gacccaaaat atagctgtta ttaaccaaat aatgcatttt tcttgataaa
     7381 tgttgattaa atcagattgg aagcaaaatg gtaatattta tgatcaattt tagtttggat
     7441 gttcaatgtc aagaccaaac cctcttcaca gctaccaact tcaattttat gtggaaaact
     7501 aatggtgaag agtttcaaac tttaactgac acttgacatt taacagggta tgtaattgct
     7561 cacacacaac ctttaggttt tgtaatccag ttttatctca tacaatatta gaaacagtac
     7621 acattgtaaa tacctatttt aaaagttagc catattttat aaagtcacat gtatttatat
     7681 cttttctgag taattttcct gttaatgcct tatgataggt gcttgtaaca tgaaaactaa
     7741 acttggatct tggcatattc agtgttctat agatagattg tgatctgtat agcttctacg
     7801 gtgactttca ggcctaatgt ctccttttaa gttgttattc tttaatatcc tttctcattc
     7861 ctttcagagg acctattatt agtttgcatt gtctgcagca tttcaataat actgttctat
     7921 tctaataata ctgatctgtt ctaataaaca gttctaaata c
//
