LOCUS       NM_001363644            5270 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens tubulin folding cofactor E like (TBCEL), transcript
            variant 3, mRNA.
ACCESSION   NM_001363644 XM_017017326
VERSION     NM_001363644.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Frederic,M.Y., Lundin,V.F., Whiteside,M.D., Cueva,J.G., Tu,D.K.,
            Kang,S.Y., Singh,H., Baillie,D.L., Hutter,H., Goodman,M.B.,
            Brinkman,F.S. and Leroux,M.R.
  TITLE     Identification of 526 conserved metazoan genetic innovations
            exposes a new role for cofactor E-like in neuronal microtubule
            homeostasis
  JOURNAL   PLoS Genet 9 (10), e1003804 (2013)
   PUBMED   24098140
REFERENCE   3  (bases 1 to 5270)
  AUTHORS   Sellin,M.E., Holmfeldt,P., Stenmark,S. and Gullberg,M.
  TITLE     Op18/Stathmin counteracts the activity of overexpressed
            tubulin-disrupting proteins in a human leukemia cell line
  JOURNAL   Exp Cell Res 314 (6), 1367-1377 (2008)
   PUBMED   18262179
  REMARK    GeneRIF: Depletion of Op18 by means of RNA interference increased
            the susceptibility of tubulin to TBCE or E-like mediated
            disruption, while overexpressed Op18 exerted a tubulin-protective
            effect.
REFERENCE   4  (bases 1 to 5270)
  AUTHORS   Bartolini,F., Tian,G., Piehl,M., Cassimeris,L., Lewis,S.A. and
            Cowan,N.J.
  TITLE     Identification of a novel tubulin-destabilizing protein related to
            the chaperone cofactor E
  JOURNAL   J Cell Sci 118 (Pt 6), 1197-1207 (2005)
   PUBMED   15728251
  REMARK    GeneRIF: Leucine rich repeat containing 35 (E-like) is a novel
            regulator of tubulin stability with overexpression causing
            depolymerization of microtubules and suppression resulting in an
            increase in the number of stable microtubules.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP001929.5 and AP000646.4.
            
            On Feb 25, 2020 this sequence version replaced NM_001363644.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.5634.1,
                                           SRR1660803.212628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000683345.1/ ENSP00000507873.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-190               AP001929.5         142455-142644
            191-298             AP001929.5         154858-154965
            299-448             AP000646.4         48340-48489
            449-588             AP000646.4         50194-50333
            589-770             AP000646.4         56217-56398
            771-1027            AP000646.4         57718-57974
            1028-1154           AP000646.4         61011-61137
            1155-1271           AP000646.4         62635-62751
            1272-5270           AP000646.4         89444-93442
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..5270
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="tubulin folding cofactor E like"
                     /db_xref="GeneID:219899"
                     /db_xref="HGNC:HGNC:28115"
                     /db_xref="MIM:610451"
     exon            1..190
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            191..298
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            299..448
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     CDS             316..1590
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="catastrophin; leucine rich repeat containing 35;
                     tubulin-specific chaperone e-like; E-like; leucine-rich
                     repeat-containing protein 35"
                     /codon_start=1
                     /product="tubulin-specific chaperone cofactor E-like
                     protein"
                     /protein_id="NP_001350573.1"
                     /db_xref="CCDS:CCDS31692.1"
                     /db_xref="GeneID:219899"
                     /db_xref="HGNC:HGNC:28115"
                     /db_xref="MIM:610451"
                     /translation="MDQPSGRSFMQVLCEKYSPENFPYRRGPGMGVHVPATPQGSPMK
                     DRLNLPSVLVLNSCGITCAGDEKEIAAFCAHVSELDLSDNKLEDWHEVSKIVSNVPQL
                     EFLNLSSNPLNLSVLERTCAGSFSGVRKLVLNNSKASWETVHMILQELPDLEELFLCL
                     NDYETVSCPSICCHSLKLLHITDNNLQDWTEIRKLGVMFPSLDTLVLANNHLNAIEEP
                     DDSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLQPYTTEERRKL
                     VIARLPSVSKLNGSVVTDGEREDSERFFIRYYVDVPQEEVPFRYHELITKYGKLEPLA
                     EVDLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSNMLLYYFDHEA
                     PFGPEEMKYSSRALHSFGIRDGDKIYVESKTK"
     exon            449..588
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            589..770
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            771..1027
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1154
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1271
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..5270
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4082..4087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="hexamer: AATAAA"
     polyA_site      4101
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="major polyA site"
     regulatory      5221..5226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="hexamer: AATATA"
     regulatory      5249..5254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="hexamer: ATTAAA"
     polyA_site      5249
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="major polyA site"
     polyA_site      5270
                     /gene="TBCEL"
                     /gene_synonym="El; LRRC35"
                     /note="major polyA site"
ORIGIN      
        1 gcttccgggt caatgcagga cactgggctc cggcggccag agtgggggac taggtgaagc
       61 ggcgccgggc cggggctggc cgggaccagg cccgaggctg agcggcggcg acagcggtgg
      121 ccgggagggg ggaggagagg cgcagccaga ggagccgccg cagtagctcc cccgcctccc
      181 gggctgaggg aatgtctaat ttaataggga tatagacagt tggtttaaac caacattttt
      241 ggacgaggag aggctatgga aatgaagaaa gaagtgttta gaaatatcac acaaacagca
      301 ttttaagaaa gaaagatgga tcaacctagt ggaagaagtt tcatgcaagt attatgtgaa
      361 aaatatagtc ctgaaaattt tccttatcgc cgtggcccgg ggatgggagt ccatgtccca
      421 gccacacctc agggctctcc tatgaaagat cgcctcaacc tcccaagtgt actagtgttg
      481 aacagctgtg gaataacctg tgcaggagat gaaaaagaaa ttgctgcttt ctgcgctcat
      541 gtgtcggaac tagatctttc tgacaacaaa ctcgaagact ggcatgaggt cagtaaaatt
      601 gtgtcaaatg ttcctcagtt ggagtttcta aacctgagtt ccaaccctct gaatttgtcg
      661 gttttagaaa gaacatgtgc tgggtccttc tctggggttc gcaaacttgt cctcaacaac
      721 agcaaagctt cttgggagac ggtccacatg atactacagg agttaccaga tttggaggag
      781 ctcttcctgt gccttaatga ctatgaaaca gtgtcttgtc cttctatttg ctgtcattct
      841 cttaagctac tacatataac agacaataac ctccaagact ggactgaaat acgaaagtta
      901 ggagttatgt ttccttcact ggataccctc gtcctggcca acaatcattt gaatgctatt
      961 gaggagcctg atgattcatt ggccaggttg tttcctaatc ttcgatccat cagcctccac
     1021 aagtcaggtt tgcagtcctg ggaagacatt gataaactaa attcatttcc caaactggaa
     1081 gaagtgagat tgttaggaat tcctcttctg cagccatata ccaccgagga gcgaaggaaa
     1141 ttggtaatag ccagattgcc atcagtttcc aaacttaatg gcagcgttgt tactgatggt
     1201 gaacgagaag attctgagag attttttatt cgttactatg tggatgttcc acaggaagaa
     1261 gtgccattca ggtatcatga actgatcact aaatatggga agttggagcc tttggcagaa
     1321 gtggacctaa gaccccagag cagtgcaaaa gtagaagtcc actttaacga tcaggtggaa
     1381 gaaatgagca ttcgtctgga ccaaacagtg gcagaactaa agaaacagtt aaaaactcta
     1441 gtacaattac ccacaagcaa catgcttctc tactattttg accatgaagc accctttggc
     1501 ccagaggaaa tgaagtacag ctctcgggca ttgcattcct ttggcattag ggatggagat
     1561 aaaatttacg tggaatccaa aacaaaataa cctctaccag ccttgtgaaa aacatacaca
     1621 taaggacttg ttgcagggca tttgttttta atgtggtttt ctttaggagg gagaggttgt
     1681 ttttgttttg ttttgttctg tttaggtttg ggaaggattt tgtatatttt tccccctgga
     1741 gtgagtaggg gccatttttg ggtgttttct accacagatt gatttggctc agccagcgga
     1801 attggccaca tttccagtgt atgtgccctc tctaaggaaa gatgacaaag aaatcaccga
     1861 cttcttactg tgttcactgg gatttgcctg ccacttggtt atcattactg ttgggtgaac
     1921 ctgtgaagat aacatgaaca ctgtagcccc ttagaagggt ctcatagaga atttaaacag
     1981 ggtgacaagg aatcttcaca ggaagggcca gaacttctct ctcccagttc ttccttccgc
     2041 taccctccct ccttggcttt tttggttcag ttccattttt ttttcatttt gacatgtggt
     2101 ttacctaata gttttgttct gttcataatt cttatttctc aacctgggtg atttttttgt
     2161 tctcatttct cccttttgaa ataattgaaa gtgttttaag catttttaac ctgattctaa
     2221 tctcaggtac gcagtaagaa gctataactc tgttagaacc tcgaagagta gatgtagaat
     2281 gaaaactcca ggaaaacttg cagttattct ggaagcaccg gcagaacagt ctgatctctt
     2341 tgtatgttac taactcactt ttaatgtccc tgtacattat gtcagccatt actttcataa
     2401 catgaaatat gtcagattcc agagttcttt tatttttgct tattgaatgt atttcctcat
     2461 atctttcttt ttcattactt taaactattg ggaattgagg cctgacttca taaataattc
     2521 aatagagtcc tggatacgtg caccaggaga gttgagaatt agctcataaa ctatctgtgg
     2581 tgtgtgtggg gaatgaagtt gggggatgtg ggggaagagc ttatgacttt cccacctgtg
     2641 tcatccattg gaagcccttg ctcttgcact ttgcattaaa agtgggaaac attaatcaaa
     2701 gggagatttt attcccaggt tttccctggg actttgtgat atcataattg aacagttctt
     2761 ttgttttaca tttcaattta gttgcctcat agaagtataa ctgcccaatc tatgagtaaa
     2821 gtgtaagtgt caaaacttta caattgcctc caagtcatat tttttgcaga agcacattta
     2881 aagcactttt ctgtaaaagc tagttcctta cctgcttgga aatctttttg tttgttcttc
     2941 tattcctttg ttaatcagat gtaatccctt tctttaatgt gtatttttct tggtccacca
     3001 tgtttacaga tgggagactt gagagatact tagcataact ggggcagaaa gtgtgtaagt
     3061 gaagtatttt tcaaagaatg cagttattat ctgattgcat ttgacctttt gacctttgtt
     3121 acataattct acccctccta caaatttaat ttttttatga aatttttagg tgacttgtaa
     3181 attcttcatg tatgaggagt tgtgtttatt aatgctactt tttaaatttt cctgtgccat
     3241 gtggcagatg tttattctct taatgcactt caggtttgct atctgtaaag cctttgaccc
     3301 aggcctactg agtcaaatct acattcagtg taacattaaa ggtggaaacc aaagggtttg
     3361 agaaagacga ataaggccta ttctccttct gctgcagact ttatctttca aaatcataaa
     3421 aatgagcaat ggagatccag gctgggtata gacaagaata attattttgc aaacacattt
     3481 tcctgacaga tttttggaag taggaaaaaa gtatggcaac agtgtcatga agattgaaac
     3541 tgtaggtgct ttgtgtatgt atgcatgagt gcagatgagt ttgagagaga aaaagtgtaa
     3601 ttgagccctt tgcttttgtc agcctgggaa acagatgcgt tcttattttt tgaagttgtg
     3661 tgaccctgga ctgtcccaca gcagaaggca gaacaaacac ttatgttatg ctttaatcat
     3721 aagtggaatg gtcacaatta ataagatatt ttatatatgg caaagtttta tgaaatgctt
     3781 ttttactatt agagacctgt ttcttctgtt attacagaac acagtgttta tcaactgcgg
     3841 acataattct tttattatac agttgcatgt aaagggagct tctcatttaa ttcagcggat
     3901 gtgggtattt ttagggcatt gtaattgatg gttttaataa ttgctgaata atttttgatt
     3961 aagagaaaaa tgtaatacaa ttactggtct gagttacaga acagaagtta atgaaaaagg
     4021 ctatttgagc atgtgtactt atagattcat ttgggtggct gagaaaagat gctgcttttg
     4081 aaataaaatt ggtgctgtgt agacacttgt aaccaaaatt atttttataa cagaactaaa
     4141 agaaggagag aagaggccat ggacctttga gtttacatgt tatacctaat tgattgtctg
     4201 cagcttatct gtattttaat attgactttg tccagattca gtgagaacat agtattgaca
     4261 ttatgaggca aaatgctgtc atagccagta ttactgatat gttgctacag acacaatgta
     4321 tagaacatac cttttctaaa ataggaatta tttccagtta tgaaaaagga aaagagtaat
     4381 aaagaaaacc aaatcaaaac caagggatcc tttctacatg tgggtgaacg gctgctaaag
     4441 ccacagtaga aaccaatttt tgaatttgaa ttgttgaaat atcctagatt gctttaatgg
     4501 aaaaaatcca gagtcacata tcttttttat tatcaagttt ttgcagcctt ttgccagcat
     4561 tgccataaat acgtaagaaa gcagcagata gtataagccc ctagttatta agacttctca
     4621 gttaatatta aggccaaaat ataacttgag ctaggtataa cagggctgaa tgctacagtg
     4681 ttgtttttaa agattttgct tatttttaaa gacaggaaaa ttcagttcta accagatgtt
     4741 ccagctatgt tactcaacca gcagtggtag gagcagagag cacttgcact aagagctgat
     4801 caccatttga gatacgcggc ttaacgcaca tgtgagtgta gcttgctaca tgaaaatgct
     4861 aggctctagg gcatgtaaaa catgaataca gaatactaga ttgttctaag taatgtcatt
     4921 tcggtttgtg aatttgattt ttcccttcat ttcatgtcat attgaaaatg caaacaaact
     4981 gctctcaaga acacccagaa gctatctgtg ttaccagatg tgttgtgaac actctactat
     5041 ttttcatagg tgcttccttg aaatatatgt ccattgtagt ggtggagagg gactggactc
     5101 tctcaggctc tttacttgcc agttgtctcc cgcagttaat ggaaatatat atacatatat
     5161 atatatattt tatttagaat ataatttaaa catgaaggtc tattctttgc actttttatt
     5221 aatatatata tagataatct ttaaaaaaat taaaattcaa gtccatttta
//
