LOCUS       NM_005993               7159 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens tubulin folding cofactor D (TBCD), transcript variant
            1, mRNA.
ACCESSION   NM_005993
VERSION     NM_005993.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Grinshpun-Cohen,J., Marans,R.S. and Singer,A.
  TITLE     [MOH SCREENING FOR TBCD IN COCHIN JEWS: COLLABORATION BETWEEN
            MEDICAL, RESEARCH AND COMMUNITY MEMBERS IN ACHIEVING PUBLIC HEALTH
            GOALS]
  JOURNAL   Harefuah 162 (6), 359-361 (2023)
   PUBMED   37394437
  REMARK    GeneRIF: [MOH SCREENING FOR TBCD IN COCHIN JEWS: COLLABORATION
            BETWEEN MEDICAL, RESEARCH AND COMMUNITY MEMBERS IN ACHIEVING PUBLIC
            HEALTH GOALS].
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 7159)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 7159)
  AUTHORS   Gronborg,S., Risom,L., Ek,J., Larsen,K.B., Scheie,D., Petkov,Y.,
            Larsen,V.A., Duno,M., Joensen,F. and Ostergaard,E.
  TITLE     A Faroese founder variant in TBCD causes early onset, progressive
            encephalopathy with a homogenous clinical course
  JOURNAL   Eur J Hum Genet 26 (10), 1512-1520 (2018)
   PUBMED   29921875
  REMARK    GeneRIF: We present a detailed description of the neuropathology
            and MR imaging characteristics of a subset of these patients,
            adding insight into the phenotype of TBCD-related encephalopathy.
            The finding of a Faroese founder variant will allow targeted
            genetic diagnostics in patients of Faroese descent as well as
            improved genetic counseling and testing of at-risk couples.
REFERENCE   5  (bases 1 to 7159)
  AUTHORS   Stephen,J., Nampoothiri,S., Vinayan,K.P., Yesodharan,D., Remesh,P.,
            Gahl,W.A. and Malicdan,M.C.V.
  TITLE     Cortical atrophy and hypofibrinogenemia due to FGG and TBCD
            mutations in a single family: a case report
  JOURNAL   BMC Med Genet 19 (1), 80 (2018)
   PUBMED   29769041
  REMARK    GeneRIF: Study identified a homozygous novel pathogenic missense
            mutation (c.1423G > A;p.Ala475Thr) in TBCD gene to be associated
            with concurrent hypofibrinogenemia and cortical atrophy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7159)
  AUTHORS   Bhamidipati,A., Lewis,S.A. and Cowan,N.J.
  TITLE     ADP ribosylation factor-like protein 2 (Arl2) regulates the
            interaction of tubulin-folding cofactor D with native tubulin
  JOURNAL   J Cell Biol 149 (5), 1087-1096 (2000)
   PUBMED   10831612
REFERENCE   7  (bases 1 to 7159)
  AUTHORS   Martin,L., Fanarraga,M.L., Aloria,K. and Zabala,J.C.
  TITLE     Tubulin folding cofactor D is a microtubule destabilizing protein
  JOURNAL   FEBS Lett 470 (1), 93-95 (2000)
   PUBMED   10722852
REFERENCE   8  (bases 1 to 7159)
  AUTHORS   Tian,G., Lewis,S.A., Feierbach,B., Stearns,T., Rommelaere,H.,
            Ampe,C. and Cowan,N.J.
  TITLE     Tubulin subunits exist in an activated conformational state
            generated and maintained by protein cofactors
  JOURNAL   J Cell Biol 138 (4), 821-832 (1997)
   PUBMED   9265649
REFERENCE   9  (bases 1 to 7159)
  AUTHORS   Tian,G., Huang,Y., Rommelaere,H., Vandekerckhove,J., Ampe,C. and
            Cowan,N.J.
  TITLE     Pathway leading to correctly folded beta-tubulin
  JOURNAL   Cell 86 (2), 287-296 (1996)
   PUBMED   8706133
REFERENCE   10 (bases 1 to 7159)
  AUTHORS   Lewis,S.A., Tian,G., Vainberg,I.E. and Cowan,N.J.
  TITLE     Chaperonin-mediated folding of actin and tubulin
  JOURNAL   J Cell Biol 132 (1-2), 1-4 (1996)
   PUBMED   8567715
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC003094.1, BI464569.1,
            BC012824.2 and AC130371.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005993.4.
            
            Summary: Cofactor D is one of four proteins (cofactors A, D, E, and
            C) involved in the pathway leading to correctly folded beta-tubulin
            from folding intermediates. Cofactors A and D are believed to play
            a role in capturing and stabilizing beta-tubulin intermediates in a
            quasi-native confirmation. Cofactor E binds to the cofactor
            D/beta-tubulin complex; interaction with cofactor C then causes the
            release of beta-tubulin polypeptides that are committed to the
            native state. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003094.1, AB023205.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355528.9/ ENSP00000347719.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               BC003094.1         2-159
            159-575             BI464569.1         156-572
            576-3418            BC003094.1         577-3419
            3419-4431           BC012824.2         877-1889
            4432-7159           AC130371.4         157664-160391       c
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..7159
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /note="tubulin folding cofactor D"
                     /db_xref="GeneID:6904"
                     /db_xref="HGNC:HGNC:11581"
                     /db_xref="MIM:604649"
     exon            1..313
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..3708
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tubulin-specific chaperone d; beta-tubulin cofactor D"
                     /codon_start=1
                     /product="tubulin-specific chaperone D isoform 1"
                     /protein_id="NP_005984.3"
                     /db_xref="CCDS:CCDS45818.1"
                     /db_xref="GeneID:6904"
                     /db_xref="HGNC:HGNC:11581"
                     /db_xref="MIM:604649"
                     /translation="MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLR
                     EVHGGGAEREVALERFRVIMDKYQEQPHLLDPHLEWMMNLLLDIVQDQTSPASLVHLA
                     FKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVT
                     CLIPFDFSRLDGNLLTQPGQARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRP
                     DVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCLPYAATV
                     LRCLDGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQ
                     GQSEQKPLILTEDDDEDDDVPEGVERVIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLP
                     RALADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLVDVVAVILKALTY
                     DEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSALVIAAVFDRDINCRR
                     AASAAFQENVGRQGTFPHGIDILTTADYFAVGNRSNCFLVISVFIAGFPEYTQPMIDH
                     LVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILA
                     CAEVAYALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCV
                     LIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSHSRQQMKDAAVSALAALCSEY
                     YMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGL
                     RAVTHTSPEDVSFAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCM
                     DDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCERIMCCVAQQASEKI
                     DRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQL
                     LGLPTYRYHVLLGLVVSLGGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIF
                     EDNLLNERVSVPLLKTLDHVLTHGCFDIFTTEEDHPFAVKLLALCKKEIKNSKDIQKL
                     LSGIAVFCEMVQFPGDVRRQALLQLCLLLCHRFPLIRKTTASQVYETLLTYSDVVGAD
                     VLDEVVTVLSDTAWDAELAVVREQRNRLCDLLGVPRPQLVPQPGAC"
     exon            314..364
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            365..462
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            463..564
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            565..711
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            712..767
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            768..900
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            901..946
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1079
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1216
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1277
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1352
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1447
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1604
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1662
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1692
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1778
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1859
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1933
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2051
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2112
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2135
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2136..2167
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2230
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2307
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2389
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2508
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2509..2600
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2738
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2822
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2823..2981
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..3120
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..3242
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3243..3320
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3321..3410
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3498
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3499..3608
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3609..3693
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..7159
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3929..3934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /note="hexamer: TATAAA"
     polyA_site      3949
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /note="major polyA site"
     regulatory      4409..4414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /note="hexamer: AATAAA"
     polyA_site      4431
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
     regulatory      7135..7140
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
                     /note="hexamer: AATAAA"
     polyA_site      7159
                     /gene="TBCD"
                     /gene_synonym="PEBAT; SSD-1; tfcD"
ORIGIN      
        1 gccagcgtcg gttgccgcct tagcgggcgc ctccttttca tccctcatcc ttcatccctg
       61 gctttcgcgc tctagcggag tgggatctgc gaacacgtga ggcgggggcg cggtccccag
      121 gctgccgaga tggccctgag cgacgaaccg gccgcgggcg gccccgagga ggaggcggag
      181 gacgagacac tggcctttgg cgcggcgctg gaagcgttcg gcgagagcgc ggagacccgg
      241 gcgctgctgg gccgcctgcg ggaggtgcac ggcggcggcg cggagcgcga ggtggccctg
      301 gagcggttcc gcgtaataat ggacaaatac caggagcagc ctcatctgtt ggacccgcac
      361 cttgaatgga tgatgaactt gttgttggac atagtgcaag atcagacatc tccagcttcc
      421 cttgtacatc tggcttttaa atttctttac atcatcacca aggttcgagg ctataaaaca
      481 tttcttcgtt tatttcctca tgaagttgcc gatgtagagc ctgttttaga tttggtcaca
      541 attcagaatc ccaaggacca tgaagcttgg gaaacccgct acatgctttt gctctggctc
      601 tccgtgacct gcctgatccc ttttgatttt tctcgccttg acgggaacct cctcacccag
      661 cctgggcaag cacgaatgtc cataatggac cgtattctcc aaatagcaga gtcctacttg
      721 attgtcagtg acaaggcccg agatgcagct gctgtccttg tgtccagatt tatcacacgt
      781 cctgatgtca agcaaagcaa gatggctgag ttcctggact ggagcctgtg caatctggcc
      841 cgttcctcct tccagaccat gcagggggtc atcaccatgg atgggacgct gcaggccctg
      901 gcacaaatat ttaaacatgg aaaacgtgaa gactgtttgc cctatgctgc cactgtcctc
      961 aggtgcctcg atggctgcag actccctgag agcaaccaga ccctgctgcg gaagctgggg
     1021 gtgaagcttg tgcagcgact ggggctgaca ttcctgaagc cgaaggtggc agcatggagg
     1081 taccagcgtg gctgccgatc tttggctgca aatctgcagc tcctcactca gggtcagagt
     1141 gagcagaagc cactcatcct gaccgaagat gacgacgaag atgacgacgt cccagagggg
     1201 gtggagcgtg tgatagagca gctgctggtc gggctgaagg acaaggacac ggtcgtgcgg
     1261 tggtctgcag ccaagggcat cggtaggatg gctggcaggc ttcccagagc cctggcggat
     1321 gatgtggtcg ggtctgtgct ggactgcttc agtttccagg agactgacaa ggcgtggcat
     1381 gggggatgtc tggcgctggc agagctgggc aggagaggcc tgttgctgcc gtctcgactc
     1441 gtggatgttg tcgccgtgat cctgaaggcg ctgacctacg acgagaagcg gggtgcctgc
     1501 agcgtgggca ccaacgtcag ggacgccgcc tgctacgtgt gctgggcctt cgcgcgtgcc
     1561 tatgagcctc aggagctgaa gccctttgtg actgcaatct cgagtgcact ggtgattgct
     1621 gcggtgtttg accgagacat aaactgcaga agagcagcct ctgccgcctt ccaggagaat
     1681 gtggggagac agggcacttt ccctcatggt attgatattt tgaccacagc tgactatttt
     1741 gccgtcggta acagatccaa ctgtttcctg gttataagtg tgtttattgc cggctttcct
     1801 gagtacacgc agccaatgat agaccacctg gttaccatga agatcagcca ctgggatggg
     1861 gtcatccgag agttggctgc gagggcgctg cacaacctgg cccagcaggc acccgagttc
     1921 agcgccacgc aagtcttccc gaggctgctg tccatgacac tgagtccaga tcttcacatg
     1981 aggcatgggt cgattctcgc ctgcgcagaa gttgcttacg ccttgtacaa acttgcagcc
     2041 caagagaaca ggcccgtcac ggaccatctg gacgagcagg cagtgcaggg cctgaagcag
     2101 attcaccagc agctctatga tcgtcagtta tacaggggtc tgggaggaca gctcatgaga
     2161 caagcagtgt gtgttttaat agaaaagttg tcactttcca aaatgccctt tagaggtgac
     2221 accgtaattg atggttggca atggctgata aatgacactt tgagacatct ccatctcatc
     2281 tcaagtcact cccgccagca gatgaaggat gcagcagtct cggccctggc tgctctatgc
     2341 agtgaatatt acatgaagga gccgggggag gcagatcccg caattcagga ggagctgatc
     2401 acgcagtacc tggctgagct tcggaacccc gaggagatga ctcgctgtgg cttctcgttg
     2461 gccttgggcg cccttccagg cttccttctg aaaggccggc tccagcaggt tctcacaggt
     2521 ttaagagcag ttacccacac ttcccccgag gacgtaagtt ttgctgagtc caggagagac
     2581 ggcttgaagg ccattgcgag gatttgccag actgttggtg tgaaagcagg agccccagac
     2641 gaagctgtgt gcggagagaa tgtttcccag atttactgtg cgctgctggg ctgcatggac
     2701 gactacacca cggacagcag aggggacgtg ggcacctggg tccgcaaggc cgccatgacc
     2761 agtctgatgg atctgacact tctgctggct cggagccagc ctgagctgat cgaggcccat
     2821 acctgtgagc gcatcatgtg ctgtgtggcc cagcaggcca gtgagaagat tgaccgtttc
     2881 cgtgctcacg ccgccagcgt gttcctgacg ctcctgcact ttgacagccc tcccatcccc
     2941 cacgtgcccc accgaggaga actggaaaag ctgtttccca ggtccgatgt ggcctccgtg
     3001 aactggagtg caccttccca ggccttccca cgcatcaccc agctccttgg gctgcccacc
     3061 taccgctacc acgtcctgct ggggctagtc gtgtccctgg gcggcttgac ggagtcgacg
     3121 atccggcact ccacccagag cctctttgag tacatgaagg gcattcagag cgacccgcag
     3181 gccctgggca gcttcagcgg gacccttctg cagatctttg aggacaacct tctgaatgag
     3241 agggtgtccg tgccgctgct gaagacgctg gaccacgtgc tcacccacgg ctgcttcgac
     3301 atcttcacca cggaggagga ccaccccttt gctgtgaagt tgcttgcgct ctgtaagaaa
     3361 gaaatcaaga attcaaaaga tatccagaag ctcctgtcag gcatcgcagt gttctgcgag
     3421 atggtgcagt tccccggcga cgtgaggagg caggccctcc tgcagctgtg tctgctcctc
     3481 tgccaccgtt tcccgctgat ccggaagacc acggccagcc aggtgtacga gacattgctc
     3541 acctacagtg acgtcgtggg cgcggatgtg ctggacgagg tggtgactgt gctcagtgac
     3601 actgcgtggg acgcggagct tgcagtggtg agagagcagc gcaaccgtct gtgtgacctt
     3661 ctgggcgtac ccaggcccca gctggtgccc cagcctggtg cctgctgaag ccagtcctgg
     3721 agcccatacc tcacccctgc ctggtgagga tgtcttgttc ctgagggagg ccggtgtgga
     3781 aagcctcgca cagtggtgcc tccagctgtt gaagggtagc gctggccctt ggaggctggc
     3841 actagctgac agcttttcct ctctgcacct gcgctctggt gacttggggt ggacgcctct
     3901 gccttcactt gaacacaaat gtgcttccta taaaatcatg taccaagaag ttcctgcctt
     3961 ttgtctctga gcctgatgtg tgtaggggtg atggaaaggc tcactgccca ggggtcagcc
     4021 agaggggagg tgggctgcac tcctcctgcc tggagaccag gcccccttct tgcctggtgc
     4081 tgtccctgct gtgggagacg tctggccaca ggcagtgccc cacccagtct tggagagaga
     4141 tgaatgttaa atcagaggtt caaatgctag agaactgaga agccctggtg gcaggcatgg
     4201 tctggagtgc tgggcagatg ctgtttctgg gcccgtctgc ctggtggggg tgctgtcctc
     4261 ccccctgtgc acacgtgagc atcgtgcatg tggcccctct gcagtgcagg agtcactgat
     4321 tgatgggtgt gccgaccagc agcttcacaa atgtctgctg caaggggaat cgtcagagtc
     4381 caatgtgtgc ctctacagta atgtcggaaa taaataccat tcttgaaata attacactgt
     4441 cttaggagag cctgggcaga gtccacactt gggtttttag gcaaagggca ggaggctacc
     4501 tttgtctctg tgtctgagct cgtgctgatc catcccaaag ctggtgaggg gaacgtgtat
     4561 aaaaatttca ctgtggtagc aacccacacc catctctggg acctgtgctg tgctcatagg
     4621 gcttgaggtg cagaaccacg gccagcagca ccaaagcagc cccaggtgtg gccactcaga
     4681 ggcccaaggg cttgggtgag caaccctttc cctatgtgag gagcccaggg gtgccatgtg
     4741 cgtggtgtag attagggtgg ctccctggcc tgctctcagg tggggatggg gcctggacac
     4801 aggaacatgg gcaggccgag aactgggagg tgccaccgca tctgtgaggc ttgtgaggtc
     4861 accaggacca caagaccagg actgtgacag tccgtgctgt gtgtttagca gacagctctt
     4921 cacgggatgt gtgtgacgac ctggccacac aggagtgtgg ggttaacaca ctactgttgt
     4981 tgtgtttgac ctggtggcca atggtcttta taccctaaaa gagccttggg ttacattttt
     5041 acgagattta aatattttat tccaacctgt gtaatagggg tctcctcctc cttgaactgt
     5101 cccgattggc tggggtcacg tggtgagggt cctgggtgac caggcctagc cttggccaca
     5161 tgagtccaca cagtggaaaa ggcttggctc ctgtggtcgg cacacacgag actctggttg
     5221 cgcctgctgg gtgtggaaca ggcgcaccat cgccatgcct gcagctcaca aagctcctgc
     5281 tggcctggga tgcactgagg atggaaggaa caagtggctt ctgagaaaaa catgatgaac
     5341 tgttcttagt gcaattaaaa gaagattcca gataaatggc attttaagac atggaggcag
     5401 gtcgattacc cacccagacc catcagtgac actatgtggc aaagcagttc ttcttttaat
     5461 gtcggtctaa cttagcaacc cgaggaaagt ggatcactcc actggccact gcctctttcc
     5521 cactggatcc catccatcac tggggcactt gtgctcacca gacacctggt ggcctggaaa
     5581 gccctctttc agcagccgtt tctggagccc accagggtcc aggtgataga gtttggcaag
     5641 gaaccccttg ctcacatgat agctggggca gacaatagca aatgaacaag caagcaccat
     5701 caaggcaggc aacactgagt gctatgaaca aagataaagt gggcaatgga atggcatgtt
     5761 gggtgttgac tccgagaagg tgttcagaaa acctctctga aggggcagca tttgggcaga
     5821 ggtccagact gtgtccaatg gcagaaaaga gaatgcttgt ggtcccagaa gtggagcaag
     5881 ctttgtgagt ttagagagca gcaagaagcc agtatccctg ggaccgggga gctgatgtgg
     5941 gatttgtgta cccacaaaca cgttctaggt gctaaccaga aaccctccat gtgagagcag
     6001 agaccttgga gatcctgagg gtttctgctg agccctggaa tctagtcacg ctattttgat
     6061 agcagaatgg atgagagaat ttaaggccca gggccagatc taatggacca ctatataaag
     6121 ctgggaccct aaataagtct ttttcaaagg aacagcagca agactcttat ctcaatctat
     6181 ctcccctgaa gtggcctgaa ttaatactac ctgtatgatc cccaaaactc ctaaagtgga
     6241 aaatttgatt gacttggccc tgaccagtgg tattcccaga caagggcaga agatgcaaat
     6301 acaaaacctc tctggtagaa tgtaggctat aatatgagta catttttgaa aagggctaga
     6361 aatgatcaag aagagattag aaaaaaacag agcccctaga aatgtaagaa gtgaaatgaa
     6421 aagctcaatg gatagcttag acacagctga agagagaatt agaaaactgg aagatctgaa
     6481 aacattatcc agagaactgg aagatatgac agcaacatca agagacattg aagaatgtct
     6541 aacctttggg taattggggt ctcagaatga aatgaaatga tgcagatatt ccatactgac
     6601 atgcaacagt ccccagatac agaagcctaa cagtctcaca tttagacctg ccacagtgaa
     6661 actgcagaac tgtaagaagg acagagtgat ataaacgact accaatttcc cagcagcagc
     6721 actggagttg agcagtgagt agaccaaggt cttcattatg ctgagagaaa atagctgtca
     6781 agttgcttga attgtgccca cagcataaca ttaaagaaga gaacaaaatt aagtcattta
     6841 gataaaaata tgccattctt atctgtttgg ttttttaatc ttggcttaat atttggggtt
     6901 gagtcatttg ttttgagagc tgtcctgttt attgcagggt gttcagcaac atcccagatg
     6961 gaagcagcat ccccctaccc agctgtgaca aaaagaaaaa aaaatgtctc cagacactgc
     7021 caaatatctt ctgggggtgc ccctggttga gaaccactgc tttagtggat aaactttagg
     7081 caggagggaa atgatcgcag ttggatagtt ggaggaatgt ggagcaaggg aagcaataaa
     7141 ctgtgaccat aaaaacata
//
