LOCUS       NM_001281                987 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens tubulin folding cofactor B (TBCB), transcript variant
            1, mRNA.
ACCESSION   NM_001281
VERSION     NM_001281.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 987)
  AUTHORS   Helferich,A.M., Brockmann,S.J., Reinders,J., Deshpande,D.,
            Holzmann,K., Brenner,D., Andersen,P.M., Petri,S., Thal,D.R.,
            Michaelis,J., Otto,M., Just,S., Ludolph,A.C., Danzer,K.M.,
            Freischmidt,A. and Weishaupt,J.H.
  TITLE     Dysregulation of a novel miR-1825/TBCB/TUBA4A pathway in sporadic
            and familial ALS
  JOURNAL   Cell Mol Life Sci 75 (23), 4301-4319 (2018)
   PUBMED   30030593
  REMARK    GeneRIF: Data indicate a microRNA-1825/TBCB/TUBA4A pathway as a
            putative pathogenic cascade in both familial ALS (fALS) and both
            sporadic (sALS).
REFERENCE   3  (bases 1 to 987)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 987)
  AUTHORS   Tan,H., Liao,H., Zhao,L., Lu,Y., Jiang,S., Tao,D., Liu,Y. and Ma,Y.
  TITLE     HILI destabilizes microtubules by suppressing phosphorylation and
            Gigaxonin-mediated degradation of TBCB
  JOURNAL   Sci Rep 7, 46376 (2017)
   PUBMED   28393858
  REMARK    GeneRIF: Our results showed that HILI suppresses microtubule
            polymerization and promotes cell proliferation, migration and
            invasion via TBCB for the first time, revealing a novel mechanism
            for HILI in tumorigenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 987)
  AUTHORS   Serna,M., Carranza,G., Martin-Benito,J., Janowski,R., Canals,A.,
            Coll,M., Zabala,J.C. and Valpuesta,J.M.
  TITLE     The structure of the complex between alpha-tubulin, TBCE and TBCB
            reveals a tubulin dimer dissociation mechanism
  JOURNAL   J Cell Sci 128 (9), 1824-1834 (2015)
   PUBMED   25908846
  REMARK    GeneRIF: the role of the human TBCE and TBCB chaperones in
            alpha-tubulin-beta-tubulin dissociation, was investigated.
REFERENCE   6  (bases 1 to 987)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   7  (bases 1 to 987)
  AUTHORS   Grimwood,J., Gordon,L.A., Olsen,A., Terry,A., Schmutz,J.,
            Lamerdin,J., Hellsten,U., Goodstein,D., Couronne,O.,
            Tran-Gyamfi,M., Aerts,A., Altherr,M., Ashworth,L., Bajorek,E.,
            Black,S., Branscomb,E., Caenepeel,S., Carrano,A., Caoile,C.,
            Chan,Y.M., Christensen,M., Cleland,C.A., Copeland,A., Dalin,E.,
            Dehal,P., Denys,M., Detter,J.C., Escobar,J., Flowers,D.,
            Fotopulos,D., Garcia,C., Georgescu,A.M., Glavina,T., Gomez,M.,
            Gonzales,E., Groza,M., Hammon,N., Hawkins,T., Haydu,L., Ho,I.,
            Huang,W., Israni,S., Jett,J., Kadner,K., Kimball,H., Kobayashi,A.,
            Larionov,V., Leem,S.H., Lopez,F., Lou,Y., Lowry,S., Malfatti,S.,
            Martinez,D., McCready,P., Medina,C., Morgan,J., Nelson,K.,
            Nolan,M., Ovcharenko,I., Pitluck,S., Pollard,M., Popkie,A.P.,
            Predki,P., Quan,G., Ramirez,L., Rash,S., Retterer,J., Rodriguez,A.,
            Rogers,S., Salamov,A., Salazar,A., She,X., Smith,D., Slezak,T.,
            Solovyev,V., Thayer,N., Tice,H., Tsai,M., Ustaszewska,A., Vo,N.,
            Wagner,M., Wheeler,J., Wu,K., Xie,G., Yang,J., Dubchak,I.,
            Furey,T.S., DeJong,P., Dickson,M., Gordon,D., Eichler,E.E.,
            Pennacchio,L.A., Richardson,P., Stubbs,L., Rokhsar,D.S.,
            Myers,R.M., Rubin,E.M. and Lucas,S.M.
  TITLE     The DNA sequence and biology of human chromosome 19
  JOURNAL   Nature 428 (6982), 529-535 (2004)
   PUBMED   15057824
REFERENCE   8  (bases 1 to 987)
  AUTHORS   Tian,G., Lewis,S.A., Feierbach,B., Stearns,T., Rommelaere,H.,
            Ampe,C. and Cowan,N.J.
  TITLE     Tubulin subunits exist in an activated conformational state
            generated and maintained by protein cofactors
  JOURNAL   J Cell Biol 138 (4), 821-832 (1997)
   PUBMED   9265649
REFERENCE   9  (bases 1 to 987)
  AUTHORS   Wolz,W., Wendelmuth,U., Rouquier,S., Giorgi,D. and Muller,C.R.
  TITLE     A complex satellite DNA polymorphism flanking the human ryanodine
            receptor gene (RYR1)
  JOURNAL   Cytogenet Cell Genet 72 (2-3), 215-216 (1996)
   PUBMED   8978780
REFERENCE   10 (bases 1 to 987)
  AUTHORS   Watanabe,T.K., Shimizu,F., Nagata,M., Kawai,A., Fujiwara,T.,
            Nakamura,Y., Takahashi,E. and Hirai,Y.
  TITLE     Cloning, expression, and mapping of CKAPI, which encodes a putative
            cytoskeleton-associated protein containing a CAP-GLY domain
  JOURNAL   Cytogenet Cell Genet 72 (2-3), 208-211 (1996)
   PUBMED   8978778
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL555345.3, D49738.1 and BC005969.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001281.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.303084.1,
                                           SRR3476690.1119410.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000221855.8/ ENSP00000221855.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AL555345.3         483-483
            2-80                D49738.1           31-109
            81-987              BC005969.1         40-946
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.12"
     gene            1..987
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /note="tubulin folding cofactor B"
                     /db_xref="GeneID:1155"
                     /db_xref="HGNC:HGNC:1989"
                     /db_xref="MIM:601303"
     exon            1..207
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..828
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytoskeleton associated protein 1; tubulin-specific
                     chaperone B; cytoskeleton-associated protein CKAPI"
                     /codon_start=1
                     /product="tubulin-folding cofactor B isoform 1"
                     /protein_id="NP_001272.2"
                     /db_xref="CCDS:CCDS12488.1"
                     /db_xref="GeneID:1155"
                     /db_xref="HGNC:HGNC:1989"
                     /db_xref="MIM:601303"
                     /translation="MEVTGVSAPTVTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELL
                     VGSPASCMELELYGVDDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGARLGEYEDVS
                     RVEKYTISQEAYDQRQDTVRSFLKRSKLGRYNEEERAQQEAEAAQRLAEEKAQASSIP
                     VGSRCEVRAAGQSPRRGTVMYVGLTDFKPGYWIGVRYDEPLGKNDGSVNGKRYFECQA
                     KYGAFVKPAVVTVGDFPEEDYGLDEI"
     exon            208..351
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            352..448
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            449..640
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            641..713
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            714..987
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      967..972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /note="hexamer: ATTAAA"
     polyA_site      987
                     /gene="TBCB"
                     /gene_synonym="CG22; CKAP1; CKAPI"
                     /note="major polyA site"
ORIGIN      
        1 gctgatagcc cagcagcagc agcggcggcg gcggctgcgg agcgggtgtg aggcggctgg
       61 accgcgctgc aggcatccgc agggcgcggc aagatggagg tgacgggggt gtcggcaccc
      121 acggtgaccg ttttcatcag cagctccctc aacaccttcc gctccgagaa gcgatacagc
      181 cgcagcctca ccatcgctga gttcaagtgt aaactggagt tgctggtggg cagccctgct
      241 tcctgcatgg aactggagct gtatggagtt gacgacaagt tctacagcaa gctggatcaa
      301 gaggatgcgc tcctgggctc ctaccctgta gatgacggct gccgcatcca cgtcattgac
      361 cacagtggcg cccgccttgg tgagtatgag gacgtgtccc gggtggagaa gtacacgatc
      421 tcacaagaag cctacgacca gaggcaagac acggtccgct ctttcctgaa gcgcagcaag
      481 ctcggccggt acaacgagga ggagcgggct cagcaggagg ccgaggccgc ccagcgcctg
      541 gccgaggaga aggcccaggc cagctccatc cccgtgggca gccgctgtga ggtgcgggcg
      601 gcgggacaat cccctcgccg gggcaccgtc atgtatgtag gtctcacaga tttcaagcct
      661 ggctactgga ttggtgtccg ctatgatgag ccactgggga aaaatgatgg cagtgtgaat
      721 gggaaacgct acttcgaatg ccaggccaag tatggcgcct ttgtcaagcc agcagtcgtg
      781 acggtggggg acttcccgga ggaggactac gggttggacg agatatgaca cctaaggaat
      841 tcccctgctt cagctcctag ctcagccact gactgcccct cctgtgtgtg cccatggccc
      901 ttttctcctg accccatttt aattttattc attttttcct ttgccattga tttttgagac
      961 tcatgcatta aattcactag aaaccca
//
