LOCUS       NM_004607                661 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens tubulin folding cofactor A (TBCA), transcript variant
            2, mRNA.
ACCESSION   NM_004607
VERSION     NM_004607.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Gordon,D.E., Hiatt,J., Bouhaddou,M., Rezelj,V.V., Ulferts,S.,
            Braberg,H., Jureka,A.S., Obernier,K., Guo,J.Z., Batra,J.,
            Kaake,R.M., Weckstein,A.R., Owens,T.W., Gupta,M., Pourmal,S.,
            Titus,E.W., Cakir,M., Soucheray,M., McGregor,M., Cakir,Z., Jang,G.,
            O'Meara,M.J., Tummino,T.A., Zhang,Z., Foussard,H., Rojc,A.,
            Zhou,Y., Kuchenov,D., Huttenhain,R., Xu,J., Eckhardt,M.,
            Swaney,D.L., Fabius,J.M., Ummadi,M., Tutuncuoglu,B., Rathore,U.,
            Modak,M., Haas,P., Haas,K.M., Naing,Z.Z.C., Pulido,E.H., Shi,Y.,
            Barrio-Hernandez,I., Memon,D., Petsalaki,E., Dunham,A.,
            Marrero,M.C., Burke,D., Koh,C., Vallet,T., Silvas,J.A.,
            Azumaya,C.M., Billesbolle,C., Brilot,A.F., Campbell,M.G.,
            Diallo,A., Dickinson,M.S., Diwanji,D., Herrera,N., Hoppe,N.,
            Kratochvil,H.T., Liu,Y., Merz,G.E., Moritz,M., Nguyen,H.C.,
            Nowotny,C., Puchades,C., Rizo,A.N., Schulze-Gahmen,U., Smith,A.M.,
            Sun,M., Young,I.D., Zhao,J., Asarnow,D., Biel,J., Bowen,A.,
            Braxton,J.R., Chen,J., Chio,C.M., Chio,U.S., Deshpande,I., Doan,L.,
            Faust,B., Flores,S., Jin,M., Kim,K., Lam,V.L., Li,F., Li,J.,
            Li,Y.L., Li,Y., Liu,X., Lo,M., Lopez,K.E., Melo,A.A., Moss,F.R.
            3rd, Nguyen,P., Paulino,J., Pawar,K.I., Peters,J.K., Pospiech,T.H.
            Jr., Safari,M., Sangwan,S., Schaefer,K., Thomas,P.V., Thwin,A.C.,
            Trenker,R., Tse,E., Tsui,T.K.M., Wang,F., Whitis,N., Yu,Z.,
            Zhang,K., Zhang,Y., Zhou,F., Saltzberg,D., Hodder,A.J.,
            Shun-Shion,A.S., Williams,D.M., White,K.M., Rosales,R., Kehrer,T.,
            Miorin,L., Moreno,E., Patel,A.H., Rihn,S., Khalid,M.M.,
            Vallejo-Gracia,A., Fozouni,P., Simoneau,C.R., Roth,T.L., Wu,D.,
            Karim,M.A., Ghoussaini,M., Dunham,I., Berardi,F., Weigang,S.,
            Chazal,M., Park,J., Logue,J., McGrath,M., Weston,S., Haupt,R.,
            Hastie,C.J., Elliott,M., Brown,F., Burness,K.A., Reid,E.,
            Dorward,M., Johnson,C., Wilkinson,S.G., Geyer,A., Giesel,D.M.,
            Baillie,C., Raggett,S., Leech,H., Toth,R., Goodman,N., Keough,K.C.,
            Lind,A.L., Klesh,R.J., Hemphill,K.R., Carlson-Stevermer,J., Oki,J.,
            Holden,K., Maures,T., Pollard,K.S., Sali,A., Agard,D.A., Cheng,Y.,
            Fraser,J.S., Frost,A., Jura,N., Kortemme,T., Manglik,A.,
            Southworth,D.R., Stroud,R.M., Alessi,D.R., Davies,P., Frieman,M.B.,
            Ideker,T., Abate,C., Jouvenet,N., Kochs,G., Shoichet,B., Ott,M.,
            Palmarini,M., Shokat,K.M., Garcia-Sastre,A., Rassen,J.A.,
            Grosse,R., Rosenberg,O.S., Verba,K.A., Basler,C.F., Vignuzzi,M.,
            Peden,A.A., Beltrao,P. and Krogan,N.J.
  CONSRTM   QCRG Structural Biology Consortium; Zoonomia Consortium
  TITLE     Comparative host-coronavirus protein interaction networks reveal
            pan-viral disease mechanisms
  JOURNAL   Science 370 (6521) (2020)
   PUBMED   33060197
REFERENCE   2  (bases 1 to 661)
  AUTHORS   Gordon,D.E., Jang,G.M., Bouhaddou,M., Xu,J., Obernier,K.,
            White,K.M., O'Meara,M.J., Rezelj,V.V., Guo,J.Z., Swaney,D.L.,
            Tummino,T.A., Huttenhain,R., Kaake,R.M., Richards,A.L.,
            Tutuncuoglu,B., Foussard,H., Batra,J., Haas,K., Modak,M., Kim,M.,
            Haas,P., Polacco,B.J., Braberg,H., Fabius,J.M., Eckhardt,M.,
            Soucheray,M., Bennett,M.J., Cakir,M., McGregor,M.J., Li,Q.,
            Meyer,B., Roesch,F., Vallet,T., Mac Kain,A., Miorin,L., Moreno,E.,
            Naing,Z.Z.C., Zhou,Y., Peng,S., Shi,Y., Zhang,Z., Shen,W.,
            Kirby,I.T., Melnyk,J.E., Chorba,J.S., Lou,K., Dai,S.A.,
            Barrio-Hernandez,I., Memon,D., Hernandez-Armenta,C., Lyu,J.,
            Mathy,C.J.P., Perica,T., Pilla,K.B., Ganesan,S.J., Saltzberg,D.J.,
            Rakesh,R., Liu,X., Rosenthal,S.B., Calviello,L., Venkataramanan,S.,
            Liboy-Lugo,J., Lin,Y., Huang,X.P., Liu,Y., Wankowicz,S.A., Bohn,M.,
            Safari,M., Ugur,F.S., Koh,C., Savar,N.S., Tran,Q.D., Shengjuler,D.,
            Fletcher,S.J., O'Neal,M.C., Cai,Y., Chang,J.C.J., Broadhurst,D.J.,
            Klippsten,S., Sharp,P.P., Wenzell,N.A., Kuzuoglu-Ozturk,D.,
            Wang,H.Y., Trenker,R., Young,J.M., Cavero,D.A., Hiatt,J.,
            Roth,T.L., Rathore,U., Subramanian,A., Noack,J., Hubert,M.,
            Stroud,R.M., Frankel,A.D., Rosenberg,O.S., Verba,K.A., Agard,D.A.,
            Ott,M., Emerman,M., Jura,N., von Zastrow,M., Verdin,E.,
            Ashworth,A., Schwartz,O., d'Enfert,C., Mukherjee,S., Jacobson,M.,
            Malik,H.S., Fujimori,D.G., Ideker,T., Craik,C.S., Floor,S.N.,
            Fraser,J.S., Gross,J.D., Sali,A., Roth,B.L., Ruggero,D.,
            Taunton,J., Kortemme,T., Beltrao,P., Vignuzzi,M., Garcia-Sastre,A.,
            Shokat,K.M., Shoichet,B.K. and Krogan,N.J.
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug
            repurposing
  JOURNAL   Nature 583 (7816), 459-468 (2020)
   PUBMED   32353859
REFERENCE   3  (bases 1 to 661)
  AUTHORS   Zhang,P., Ma,X., Song,E., Chen,W., Pang,H., Ni,D., Gao,Y., Fan,Y.,
            Ding,Q., Zhang,Y. and Zhang,X.
  TITLE     Tubulin cofactor A functions as a novel positive regulator of ccRCC
            progression, invasion and metastasis
  JOURNAL   Int J Cancer 133 (12), 2801-2811 (2013)
   PUBMED   23740643
  REMARK    GeneRIF: overexpression of TBCA did not induce biocharacteristics
            that directly contrasted to those of TBCA knockdown
REFERENCE   4  (bases 1 to 661)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   5  (bases 1 to 661)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   6  (bases 1 to 661)
  AUTHORS   Nolasco,S., Bellido,J., Goncalves,J., Zabala,J.C. and Soares,H.
  TITLE     Tubulin cofactor A gene silencing in mammalian cells induces
            changes in microtubule cytoskeleton, cell cycle arrest and cell
            death
  JOURNAL   FEBS Lett 579 (17), 3515-3524 (2005)
   PUBMED   15963512
  REMARK    GeneRIF: TBCA is essential for cell viability and its knockdown
            produces a decrease in the amount of soluble tubulin, modifications
            in microtubules and G1 cell cycle arrest.
REFERENCE   7  (bases 1 to 661)
  AUTHORS   Guasch,A., Aloria,K., Perez,R., Avila,J., Zabala,J.C. and Coll,M.
  TITLE     Three-dimensional structure of human tubulin chaperone cofactor A
  JOURNAL   J Mol Biol 318 (4), 1139-1149 (2002)
   PUBMED   12054808
REFERENCE   8  (bases 1 to 661)
  AUTHORS   Mao,M., Fu,G., Wu,J.S., Zhang,Q.H., Zhou,J., Kan,L.X., Huang,Q.H.,
            He,K.L., Gu,B.W., Han,Z.G., Shen,Y., Gu,J., Yu,Y.P., Xu,S.H.,
            Wang,Y.X., Chen,S.J. and Chen,Z.
  TITLE     Identification of genes expressed in human CD34(+) hematopoietic
            stem/progenitor cells by expressed sequence tags and efficient
            full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 95 (14), 8175-8180 (1998)
   PUBMED   9653160
REFERENCE   9  (bases 1 to 661)
  AUTHORS   Tian,G., Huang,Y., Rommelaere,H., Vandekerckhove,J., Ampe,C. and
            Cowan,N.J.
  TITLE     Pathway leading to correctly folded beta-tubulin
  JOURNAL   Cell 86 (2), 287-296 (1996)
   PUBMED   8706133
REFERENCE   10 (bases 1 to 661)
  AUTHORS   Lewis,S.A., Tian,G., Vainberg,I.E. and Cowan,N.J.
  TITLE     Chaperonin-mediated folding of actin and tubulin
  JOURNAL   J Cell Biol 132 (1-2), 1-4 (1996)
   PUBMED   8567715
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC018210.1, DB538817.1 and
            AC026717.4.
            
            On Feb 27, 2020 this sequence version replaced NM_004607.2.
            
            Summary: The product of this gene is one of four proteins
            (cofactors A, D, E, and C) involved in the pathway leading to
            correctly folded beta-tubulin from folding intermediates. Cofactors
            A and D are believed to play a role in capturing and stabilizing
            beta-tubulin intermediates in a quasi-native confirmation. Cofactor
            E binds to the cofactor D/beta-tubulin complex; interaction with
            cofactor C then causes the release of beta-tubulin polypeptides
            that are committed to the native state. This gene encodes
            chaperonin cofactor A. Multiple alternatively spliced transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) has a split 3' terminal exon,
            which results in an alternate translation stop codon, compared to
            variant 1. The resulting isoform (2) is shorter and has a distinct
            C-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ891522.1, BC018210.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380377.9/ ENSP00000369736.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-581               BC018210.1         8-588
            582-657             DB538817.1         367-442
            658-661             AC026717.4         15491-15494         c
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.1"
     gene            1..661
                     /gene="TBCA"
                     /note="tubulin folding cofactor A"
                     /db_xref="GeneID:6902"
                     /db_xref="HGNC:HGNC:11579"
                     /db_xref="MIM:610058"
     exon            1..135
                     /gene="TBCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..409
                     /gene="TBCA"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tubulin-specific chaperone a; chaperonin cofactor A; CFA;
                     TCP1-chaperonin cofactor A; epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="tubulin-specific chaperone A isoform 2"
                     /protein_id="NP_004598.1"
                     /db_xref="CCDS:CCDS4040.1"
                     /db_xref="GeneID:6902"
                     /db_xref="HGNC:HGNC:11579"
                     /db_xref="MIM:610058"
                     /translation="MADPRVRQIKIKTGVVKRLVKEKVMYEKEAKQQEEKIEKMRAED
                     GENYDIKKQAEILQESRMMIPDCQRRLEAAYLDLQRILENEKDLEEAEEYKEARLVLD
                     SVKLEA"
     exon            136..241
                     /gene="TBCA"
                     /inference="alignment:Splign:2.1.0"
     exon            242..328
                     /gene="TBCA"
                     /inference="alignment:Splign:2.1.0"
     exon            329..661
                     /gene="TBCA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      580..585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCA"
                     /note="hexamer: AATAAA"
     polyA_site      605
                     /gene="TBCA"
                     /note="major polyA site"
     regulatory      632..637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBCA"
                     /note="hexamer: ATTAAA"
     polyA_site      661
                     /gene="TBCA"
ORIGIN      
        1 ctaaatagcc gcagcctctg cgcgtcgccc tccacggtta ccccggctct ccgcccctcc
       61 ttctcgcggc gctcgaggga ccatggccga tcctcgcgtg agacagatca agatcaagac
      121 cggcgtggtg aagcggttgg tcaaagaaaa agtgatgtat gaaaaagagg caaaacaaca
      181 agaagaaaag attgaaaaaa tgagagctga agacggtgaa aattatgaca ttaaaaagca
      241 ggcagagatc ctacaagaat ccaggatgat gatcccagat tgccagcgca ggttggaagc
      301 cgcatatttg gatcttcaac ggatactaga aaatgaaaaa gacttggaag aagctgagga
      361 atataaagaa gcacgtttag tactggattc agtgaagtta gaagcctgaa acttttctcg
      421 tatggggtgg tttttgcatt aaatcctggg gtccatttta caatccatta tttttgacca
      481 ctgctatgtg ttcaagtagt atgagaatgt gattgttttt atctggttac atatatattt
      541 ctttgtctaa tttaatatgt caaataaatg agttcatcta ataaaattgt ttattttata
      601 ctttacaaaa tttttaaatt aaccttttat cattaaacca catagacttt atgacagaga
      661 a
//
