LOCUS       NM_017752               5729 bp    mRNA    linear   PRI 23-APR-2024
DEFINITION  Homo sapiens TBC1 domain family member 8B (TBC1D8B), transcript
            variant 1, mRNA.
ACCESSION   NM_017752
VERSION     NM_017752.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Zhao,H., Liu,L.L., Sun,J., Jin,L., Xie,H.B., Li,J.B., Xu,H.,
            Wu,D.D., Zhuang,X.L., Peng,M.S., Guo,Y.J., Qian,W.Z., Otecko,N.O.,
            Sun,W.J., Qu,L.H., He,J., Chen,Z.L., Liu,R., Chen,C.S. and
            Zhang,Y.P.
  TITLE     A human-specific insertion promotes cell proliferation and
            migration by enhancing TBC1D8B expression
  JOURNAL   Sci China Life Sci 67 (4), 765-777 (2024)
   PUBMED   38110796
  REMARK    GeneRIF: A human-specific insertion promotes cell proliferation and
            migration by enhancing TBC1D8B expression.
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Okada,J., Matsumoto,S., Yamada,E., Saito,T., Okada,K., Watanabe,T.,
            Nakajima,Y., Ozawa,A., Yamada,M., Ohshima,K. and Okada,S.
  TITLE     TBC1D8B, a GTPase-activating protein, is a novel apoptosis inducer
  JOURNAL   Biomed Res 42 (3), 95-102 (2021)
   PUBMED   34092754
  REMARK    GeneRIF: TBC1D8B, a GTPase-activating protein, is a novel apoptosis
            inducer.
REFERENCE   3  (bases 1 to 5729)
  AUTHORS   Kampf,L.L., Schneider,R., Gerstner,L., Thunauer,R., Chen,M.,
            Helmstadter,M., Amar,A., Onuchic-Whitford,A.C., Loza Munarriz,R.,
            Berdeli,A., Muller,D., Schrezenmeier,E., Budde,K., Mane,S.,
            Laricchia,K.M., Rehm,H.L., MacArthur,D.G., Lifton,R.P., Walz,G.,
            Romer,W., Bergmann,C., Hildebrandt,F. and Hermle,T.
  TITLE     TBC1D8B Mutations Implicate RAB11-Dependent Vesicular Trafficking
            in the Pathogenesis of Nephrotic Syndrome
  JOURNAL   J Am Soc Nephrol 30 (12), 2338-2353 (2019)
   PUBMED   31732614
  REMARK    GeneRIF: TBC1D8B mutations were found in 5 families with nephrotic
            syndrome: 2 premature stop codons (c.1030C>T, p.Arg344* and
            c.1383G>A, p.Trp461*), 3 missense mutations (c.2338A>T,
            p.Thr780Ser, c.1316T>G, p.Phe439Cys, and c.190C>T, p.Arg64Cys).
            TBC1D8B plays a regulatory role by inhibiting endogenous RAB11. It
            interacts with nephrin.
REFERENCE   4  (bases 1 to 5729)
  AUTHORS   Dorval,G., Kuzmuk,V., Gribouval,O., Welsh,G.I., Bierzynska,A.,
            Schmitt,A., Miserey-Lenkei,S., Koziell,A., Haq,S., Benmerah,A.,
            Mollet,G., Boyer,O., Saleem,M.A. and Antignac,C.
  TITLE     TBC1D8B Loss-of-Function Mutations Lead to X-Linked Nephrotic
            Syndrome via Defective Trafficking Pathways
  JOURNAL   Am J Hum Genet 104 (2), 348-355 (2019)
   PUBMED   30661770
  REMARK    GeneRIF: results confirmed that pathogenic variations in TBC1D8B
            are involved in X-linked podocytopathy and points to alterations in
            recycling processes as a mechanism of steroid-resistant nephrotic
            syndrome
REFERENCE   5  (bases 1 to 5729)
  AUTHORS   Whitcomb,D.C., LaRusch,J., Krasinskas,A.M., Klei,L., Smith,J.P.,
            Brand,R.E., Neoptolemos,J.P., Lerch,M.M., Tector,M., Sandhu,B.S.,
            Guda,N.M., Orlichenko,L., Alkaade,S., Amann,S.T., Anderson,M.A.,
            Baillie,J., Banks,P.A., Conwell,D., Cote,G.A., Cotton,P.B.,
            DiSario,J., Farrer,L.A., Forsmark,C.E., Johnstone,M., Gardner,T.B.,
            Gelrud,A., Greenhalf,W., Haines,J.L., Hartman,D.J., Hawes,R.A.,
            Lawrence,C., Lewis,M., Mayerle,J., Mayeux,R., Melhem,N.M.,
            Money,M.E., Muniraj,T., Papachristou,G.I., Pericak-Vance,M.A.,
            Romagnuolo,J., Schellenberg,G.D., Sherman,S., Simon,P., Singh,V.P.,
            Slivka,A., Stolz,D., Sutton,R., Weiss,F.U., Wilcox,C.M.,
            Zarnescu,N.O., Wisniewski,S.R., O'Connell,M.R., Kienholz,M.L.,
            Roeder,K., Barmada,M.M., Yadav,D. and Devlin,B.
  CONSRTM   Alzheimer's Disease Genetics Consortium
  TITLE     Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter
            risk for alcohol-related and sporadic pancreatitis
  JOURNAL   Nat Genet 44 (12), 1349-1354 (2012)
   PUBMED   23143602
REFERENCE   6  (bases 1 to 5729)
  AUTHORS   Ishibashi,K., Kanno,E., Itoh,T. and Fukuda,M.
  TITLE     Identification and characterization of a novel Tre-2/Bub2/Cdc16
            (TBC) protein that possesses Rab3A-GAP activity
  JOURNAL   Genes Cells 14 (1), 41-52 (2009)
   PUBMED   19077034
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL391315.22, AB449890.1,
            AL591849.7 and BQ014086.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_017752.2.
            
            Summary: This gene encodes a protein with a TBC (Tre-2/Bub2/CDC16)
            domain. Some mammalian proteins with this domain have been shown to
            function as Rab-GAPs by binding to specific Rab proteins and
            affecting their GTPase activity. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.166150.1,
                                           SRR1660809.222380.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357242.10/ ENSP00000349781.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-181               AL391315.22        99857-100037
            182-3544            AB449890.1         1-3363
            3545-5032           AL591849.7         3338-4825
            5033-5729           BQ014086.1         14-710              c
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.3"
     gene            1..5729
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /note="TBC1 domain family member 8B"
                     /db_xref="GeneID:54885"
                     /db_xref="HGNC:HGNC:24715"
                     /db_xref="MIM:301027"
     exon            1..311
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..3544
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /note="isoform a is encoded by transcript variant 1;
                     RP11-321G1.1; TBC1 domain family, member 8B (with GRAM
                     domain)"
                     /codon_start=1
                     /product="TBC1 domain family member 8B isoform a"
                     /protein_id="NP_060222.2"
                     /db_xref="CCDS:CCDS14522.1"
                     /db_xref="GeneID:54885"
                     /db_xref="HGNC:HGNC:24715"
                     /db_xref="MIM:301027"
                     /translation="MWLKPEEVLLKNALKLWLMERSNDYFVLQRRRGYGEEGGGGLTG
                     LLVGTLDSVLDSTAKVAPFRILHQTPDSQVYLSIACGANREEITKHWDWLEQNIMKTL
                     SVFDSNEDITNFVQGKIRGLIAEEGKHCFAKEDDPEKFREALLKFEKCFGLPEKEKLV
                     TYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTE
                     SIHVCSQGENHYFSMFLHINQTYLLMEQLANYAIRRLFDKETFDNDPVLYNPLQITKR
                     GLENRAHSEQFNAFFRLPKGESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDG
                     NQCSVIIPLREVLAIDKTNDSSKSVIISIKGKTAFRFHEVKDFEQLVAKLRLRCGAAS
                     TQYHDISTELAISSESTEPSDNFEVQSLTSQRECSKTVNTEALMTVFHPQNLETLNSK
                     MLKEKMKEQSWKILFAECGRGVSMFRTKKTRDLVVRGIPETLRGELWMLFSGAVNDMA
                     TNPDYYTEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTGISALRRVLTAYAYRN
                     PKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEEL
                     IRDHLPQLTEHMTDMTFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLG
                     LAILDYNLDKLLTCKDDAEAVTALNRFFDNVTNKDSPLPSNVQQGSNVSDEKTSHTRV
                     DITDLIRESNEKYGNIRYEDIHSMRCRNRLYVIQTLEETTKQNVLRVVSQDVKLSLQE
                     LDELYVIFKKELFLSCYWCLGCPVLKHHDPSLPYLEQYQIDCQQFRALYHLLSPWAHS
                     ANKDSLALWTFRLLDENSDCLINFKEFSSAIDIMYNGSFTEKLKLLFKLHIPPAYTEV
                     KSKDASKGDELSKEELLYFSQLHVSKPANEKEAESAKHSPEKGKGKIDIQAYLSQWQD
                     ELFKKEENIKDLPRMNQSQFIQFSKTLYNLFHEDPEEESLYQAIAVVTSLLLRMEEVG
                     RKLHSPTSSAKGFSGTVCGSGGPSEEKTGSHLEKDPCSFREEPQWSFAFEQILASLLN
                     EPALVRFFEKPIDVKAKLENARISQLRSRTKM"
     exon            312..422
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            423..541
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            542..767
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            768..1008
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1216
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1384
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1534
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1685
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1900
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2018
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2304
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2434
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2533
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2602
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2843
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2844..2909
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..2993
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..3050
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3148
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..5729
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5706..5711
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /note="hexamer: AATATA"
     polyA_site      5729
                     /gene="TBC1D8B"
                     /gene_synonym="GRAMD8B; NPHS20"
                     /note="major polyA site"
ORIGIN      
        1 ggcaatctct ctgcctacgt gggagagaag ggagggttgg gggaagtgtg gaaaacctga
       61 acctgagctg ctgtcgcctg aggaagattt ggtgggagga gaagcagagg ggaagagacg
      121 ggttgagagt gaggtgagga gggcatctag gtcactgctc ccggggggca caaagttcgc
      181 gatgtggctg aagcctgagg aagtgcttct gaaaaatgcg ctgaagctgt ggctgatgga
      241 aaggtccaac gactacttcg tgctgcagcg gcgtcggggc tacggggagg aaggcggagg
      301 ggggctcaca gggcttctgg ttgggactct tgattcagtc ttggactcta ctgctaaagt
      361 agctccattt cgcatcctac accagacacc agattctcaa gtttacttgt caattgcatg
      421 tggagccaac agagaagaaa taaccaagca ttgggattgg ttggaacaaa atattatgaa
      481 gaccttatct gtatttgatt caaatgaaga tattactaat tttgtacaag gaaaaataag
      541 aggattaatt gctgaagagg gaaaacattg ttttgcaaaa gaagatgatc ctgagaaatt
      601 tcgagaagcc cttttgaaat ttgaaaaatg ttttggttta ccagagaagg agaagttagt
      661 gacctattat tcatgcagtt attggaaagg acgggttcct tgtcagggtt ggctttatct
      721 tagcaccaac tttctgagct tctattcttt tttgttggga tcagaaataa aactcattat
      781 ctcctgggat gaagtctcaa aacttgaaaa gacttcaaat gtcatactga cagagagtat
      841 tcacgtgtgt tcccaaggag agaatcacta cttttcaatg tttttgcaca ttaaccaaac
      901 ataccttctt atggaacagc tggcaaacta tgccattaga agactttttg ataaggaaac
      961 atttgataat gacccagtcc tttataatcc tctacagatc accaaaagag gtctggaaaa
     1021 tagagcccac agtgagcaat ttaatgcctt ttttaggctg cccaaaggag agagtttgaa
     1081 agaagtacat gaatgtttct tatgggtacc attcagccac ttcaatactc atgggaaaat
     1141 gtgcatctca gaaaattata tctgctttgc tagccaagat ggcaatcagt gtagtgtaat
     1201 cattccacta cgagaggtct tagctataga taagacaaat gattccagca aatctgtcat
     1261 cattagcatc aaaggaaaaa cagcttttcg cttccatgaa gttaaagact ttgaacaact
     1321 ggtagcaaaa ctcaggctca gatgcggagc agcttcaact caatatcatg atattagcac
     1381 agagcttgct attagttctg agtctacaga gccatctgat aattttgagg tgcaatcttt
     1441 gacaagtcag agggaatgca gtaaaactgt gaacactgaa gccttaatga cagtatttca
     1501 ccctcagaat ttggagactc ttaattctaa aatgttgaaa gaaaaaatga aggaacagtc
     1561 atggaaaata ctgtttgcag aatgtggacg tggtgttagt atgtttcgaa ccaaaaagac
     1621 tcgagatctt gttgtaagag ggattccaga aacattaaga ggagaactct ggatgctttt
     1681 ttcaggtgct gttaatgaca tggctactaa tcctgactat tatactgaag tggttgagca
     1741 gtccttaggg acctgcaact tggctactga agaaattgaa cgtgatttac gtcgctctct
     1801 gcctgagcac ccagcctttc agagtgatac tggcatatct gctctgagaa gggtactcac
     1861 agcttatgca tacaggaatc ccaaaattgg atactgccag gcaatgaata ttttgacttc
     1921 agtgctgctt ctatatgcaa aagaggaaga agctttttgg cttctggttg ctgtatgtga
     1981 acgaatgttg cctgattatt ttaatcgtcg aattattggt gccttggtgg atcaggcagt
     2041 ctttgaagaa cttatcaggg atcaccttcc tcagctgaca gaacacatga ctgatatgac
     2101 attcttttcc tcagtttctc tctcttggtt tctcacactt tttattagtg tgctacctat
     2161 tgaaagtgca gtgaatgtgg tggactgttt cttctatgat ggaataaagg ccattttgca
     2221 actgggattg gcaatacttg actataattt agacaaactg ctgacttgta aagatgatgc
     2281 tgaagctgtg acagccttaa acaggttctt tgacaatgtc actaataagg atagtccatt
     2341 gccttcaaat gttcagcaag gttcaaatgt gagtgatgaa aaaaccagtc atactagagt
     2401 ggatattaca gatttgatta gagaatcaaa tgagaaatat ggtaatattc gctatgaaga
     2461 tatacatagt atgcgctgtc gaaataggtt gtatgtgata cagaccctag aggaaacaac
     2521 aaaacagaat gtgttgcgtg ttgtatcaca agatgtgaaa ttgagccttc aagaattgga
     2581 tgaactttat gtcatcttta agaaagagct gtttttatct tgttattggt gtttgggttg
     2641 cccagtattg aagcatcatg accccagtct gccatatttg gaacagtatc agattgactg
     2701 ccagcagttc agagcgttgt atcacttgtt gagtccctgg gctcattctg caaataaaga
     2761 ctcactagct ttatggacat tcagattgtt agatgaaaac tctgattgcc ttataaactt
     2821 caaagaattc tcctctgcaa ttgacataat gtacaatgga agttttactg agaagcttaa
     2881 gctgcttttt aagctacata ttcctccagc ttacactgaa gtgaaatcta aggatgcttc
     2941 aaaaggagat gaactttcca aggaagaatt actttatttc agtcagctgc atgtttccaa
     3001 gcctgcaaat gagaaggaag cagaatcagc aaaacacagc cctgaaaaag gcaaagggaa
     3061 aattgatatt caagcatatc taagtcaatg gcaagatgag cttttcaaaa aagaagaaaa
     3121 cattaaggat ttaccaagaa tgaatcagtc tcagtttatt cagttttcaa agaccctcta
     3181 taacttattt catgaggacc ctgaagaaga atcattatat caagccattg ctgttgtaac
     3241 cagcctttta ctcaggatgg aagaagttgg aaggaaacta catagcccta catcatcagc
     3301 caaaggattc tctggtactg tctgtggttc tggaggaccc agtgaggaaa aaacagggag
     3361 ccacttggag aaagatcctt gttcctttag ggaggaacct cagtggtcat ttgcatttga
     3421 acagattctt gcatcgctgt tgaatgaacc agcattggtg aggttttttg agaaacccat
     3481 agatgtaaaa gccaagctgg aaaatgcaag aatttctcag ttaaggtcta gaaccaagat
     3541 gtaaatccct aggaattgcc tatcatagac aagtttacta acattcctgt agctgtcagt
     3601 ttgattcctg tgagtagggc tcagggattt atcttgttac caatgtgtct gaaggccaaa
     3661 atatatatcc agaagcacaa tgcatcattc ctttgttgtt gataatgggc tttgttagca
     3721 ctttttaaaa caaacaaaca aacaaaacaa aaaagcaaac cacatttgtt atctcaaatt
     3781 ttgatgatat tctcaaatac aaatatactt ttttatattt cacaatatat gcaatatcag
     3841 gggaatatgc taaatgttac caccagaggg cacaagcata tcacttttag taaggaaatt
     3901 actagcttgt gttgctattt acatatgaat tactgattat tttgaaaaga cggtgttatg
     3961 cttgggtgtt agtgaggctg atatgtcatg tcaatcgata aaccctactt tccaaattat
     4021 ctaaaagatt accatttggg gcccttgttt tcaagttatc taactgaaat attattaatt
     4081 tttttaagtt aatctcattc agtcttctag taataaatgc ttttttaagt accttctaca
     4141 gtcttcctac ctgaggggtt cagaggctgc tgactcacac tgctaactct gttactgaca
     4201 aaagcaaatc aataactcta aattctgggc tgatataaag aaaaaaacaa tattggattt
     4261 ggatttaatc tgggtaaaat cagccttctg agaagggcta agaaacagtt caattaactg
     4321 tataggtctt tcattagacc gagacatcac tagaaattct gggcactgct gtttgtggta
     4381 tttgtctcag agtagcttat gactgttttg gttctagctt tagattttgg attttcacca
     4441 gtcatgtgtt aatttacatt agatagtaaa gaatggctac actaagactg aattatctta
     4501 ttagtctggc aagcatataa agtatacttg tatgtaatga ttgccaaggt aacagaattg
     4561 ctgacatctg tctaaaaagt tttgactatc caatttagat gtgtaatttc tatatacttt
     4621 atgctgttta tatttcagtc attacaccaa gtccatttag agataatggg ctttgttagc
     4681 actttcaaaa aaaaaaaaag caaaccatac ttgtccactg tcctgtgcta ttcctaccaa
     4741 ggacaattta ttaatcaatt gacttagaaa atagggggag aaatcattca ttggagcttc
     4801 tttctttaga aaagactttg gttataagcc ttttgacttt tctagatgtg aaaccatgaa
     4861 agggcagacc tccttcagag tgactcaaga ggtctccctt tcttggaggg agttggtaca
     4921 gtcagccttt gggaagaatc cactgtgaac aaagcttaac acatggggct tcatcgctca
     4981 tagaatatgt tattttcaaa gaagttcaag aattttcaag ttgagccttt gaaaatccca
     5041 taaattggtt ttagctaaac acttactagt agtgtcttta aattatttaa tcaaccttgt
     5101 cttttcaagg aaattaccac ttaaagagat agttggtaaa taaacatcta tgccttttct
     5161 cagaaatgat ttgctgaact atgtccatat tttacagctt agataatagt ttatatggaa
     5221 actattatac atctgctatt gtgcaatgat tgttaaatta tactgaagta gctctagaaa
     5281 gacacatgta tacaaggcac tattgtacac actttgctga atattttgtc agttgtattt
     5341 acaaagaaag gtactttctt aagagcatat atgttattaa tatttgatat gattttaaag
     5401 tcagaatagt acagattgct gagtattata ctttaggcta gattaattaa aattgaatac
     5461 tgaaagagat tttttgagtt gcaaaaagtt tataaatgca aagcaaaaag aaaacattta
     5521 ttttctgagt ctgcaggaga aacaaactaa acattatagt tttatagctg ctatcttgtt
     5581 aaccaaacag gttgttcata atattaaaaa tcttacgtag ttgtgttaaa ctgaaccagt
     5641 tcattatacc ttatgcatta aattaaatat gttataaggt ggctttactt gtctttataa
     5701 aaataaatat atctactaaa catgaaaaa
//
