LOCUS       NM_014832               7588 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens TBC1 domain family member 4 (TBC1D4), transcript
            variant 1, mRNA.
ACCESSION   NM_014832 XM_375032
VERSION     NM_014832.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Wu,N., Jing,Z., Lv,H., Liu,Q., Gu,M., Zhong,Y., Xing,P., Ma,R. and
            Jing,Y.
  TITLE     Expression characteristics of TBC1D4 activating protein molecule
            and identification of key module genes for preventing thyroid
            cancer progression
  JOURNAL   Int J Biol Macromol 278 (Pt 4), 134986 (2024)
   PUBMED   39181362
  REMARK    GeneRIF: Expression characteristics of TBC1D4 activating protein
            molecule and identification of key module genes for preventing
            thyroid cancer progression.
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Lewis,J.I., Lind,M.V., Moller,G., Hansen,T., Pedersen,H.,
            Christensen,M.M.B., Laursen,J.C., Nielsen,S., Ottendahl,C.B.,
            Larsen,C.V.L., Stark,K.D., Bjerregaard,P., Jorgensen,M.E. and
            Lauritzen,L.
  TITLE     The effect of traditional diet on glucose homoeostasis in carriers
            and non-carriers of a common TBC1D4 variant in Greenlandic Inuit: a
            randomised crossover study
  JOURNAL   Br J Nutr 130 (11), 1871-1884 (2023)
   PUBMED   37129117
  REMARK    GeneRIF: The effect of traditional diet on glucose homoeostasis in
            carriers and non-carriers of a common TBC1D4 variant in Greenlandic
            Inuit: a randomised crossover study.
REFERENCE   3  (bases 1 to 7588)
  AUTHORS   Overvad,M., Diaz,L.J., Bjerregaard,P., Pedersen,M.L.,
            Larsen,C.V.L., Grarup,N., Hansen,T., Rossing,P. and Jorgensen,M.E.
  TITLE     The effect of diabetes and the diabetogenic TBC1D4 p.Arg684ter
            variant on kidney function in Inuit in Greenland
  JOURNAL   Int J Circumpolar Health 82 (1), 2191406 (2023)
   PUBMED   36944026
  REMARK    GeneRIF: The effect of diabetes and the diabetogenic TBC1D4
            p.Arg684ter variant on kidney function in Inuit in Greenland.
REFERENCE   4  (bases 1 to 7588)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 7588)
  AUTHORS   Koumanov,F., Richardson,J.D., Murrow,B.A. and Holman,G.D.
  TITLE     AS160 phosphotyrosine-binding domain constructs inhibit
            insulin-stimulated GLUT4 vesicle fusion with the plasma membrane
  JOURNAL   J Biol Chem 286 (19), 16574-16582 (2011)
   PUBMED   21454690
  REMARK    GeneRIF: AS160 phosphotyrosine-binding domain constructs inhibit
            insulin-stimulated GLUT4 vesicle fusion with the plasma membrane
REFERENCE   6  (bases 1 to 7588)
  AUTHORS   Dash,S., Sano,H., Rochford,J.J., Semple,R.K., Yeo,G., Hyden,C.S.,
            Soos,M.A., Clark,J., Rodin,A., Langenberg,C., Druet,C.,
            Fawcett,K.A., Tung,Y.C., Wareham,N.J., Barroso,I., Lienhard,G.E.,
            O'Rahilly,S. and Savage,D.B.
  TITLE     A truncation mutation in TBC1D4 in a family with acanthosis
            nigricans and postprandial hyperinsulinemia
  JOURNAL   Proc Natl Acad Sci U S A 106 (23), 9350-9355 (2009)
   PUBMED   19470471
  REMARK    GeneRIF: Prematurely truncated TBC1D4 protein tended to increase
            basal cell membrane GLUT4 levels (P = 0.053) and significantly
            reduced insulin-stimulated GLUT4 cell membrane translocation (P <
            0.05).
REFERENCE   7  (bases 1 to 7588)
  AUTHORS   Baus,D., Heermeier,K., De Hoop,M., Metz-Weidmann,C.,
            Gassenhuber,J., Dittrich,W., Welte,S. and Tennagels,N.
  TITLE     Identification of a novel AS160 splice variant that regulates GLUT4
            translocation and glucose-uptake in rat muscle cells
  JOURNAL   Cell Signal 20 (12), 2237-2246 (2008)
   PUBMED   18771725
  REMARK    GeneRIF: The transcript variant 2 of AS160 in contrast to
            full-length AS160 is a novel regulator of glucose transport that
            positively influences glucose-uptake rates.
REFERENCE   8  (bases 1 to 7588)
  AUTHORS   Matsumoto,Y., Imai,Y., Lu Yoshida,N., Sugita,Y., Tanaka,T.,
            Tsujimoto,G., Saito,H. and Oshida,T.
  TITLE     Upregulation of the transcript level of GTPase activating protein
            KIAA0603 in T cells from patients with atopic dermatitis
  JOURNAL   FEBS Lett 572 (1-3), 135-140 (2004)
   PUBMED   15304337
  REMARK    GeneRIF: KIAA0603 is likely to be a Rab GAP that participates in
            the regulation of activated T cells in atopic dermatitis
REFERENCE   9  (bases 1 to 7588)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   10 (bases 1 to 7588)
  AUTHORS   Kane,S., Sano,H., Liu,S.C., Asara,J.M., Lane,W.S., Garner,C.C. and
            Lienhard,G.E.
  TITLE     A method to identify serine kinase substrates. Akt phosphorylates a
            novel adipocyte protein with a Rab GTPase-activating protein (GAP)
            domain
  JOURNAL   J Biol Chem 277 (25), 22115-22118 (2002)
   PUBMED   11994271
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139230.25, AK307493.1,
            AB011175.1, FM207106.1, AL162571.9 and CA448893.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_014832.4.
            
            Summary: This gene is a member of the Tre-2/BUB2/CDC16 domain
            family. The protein encoded by this gene is a Rab-GTPase-activating
            protein, and contains two phopshotyrosine-binding domains (PTB1 and
            PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and
            multiple AKT phosphomotifs. This protein is thought to play an
            important role in glucose homeostasis by regulating the
            insulin-dependent trafficking of the glucose transporter 4 (GLUT4),
            important for removing glucose from the bloodstream into skeletal
            muscle and fat tissues. Reduced expression of this gene results in
            an increase in GLUT4 levels at the plasma membrane, suggesting that
            this protein is important in intracellular retention of GLUT4 under
            basal conditions. When exposed to insulin, this protein is
            phosphorylated, dissociates from GLUT4 vesicles, resulting in
            increased GLUT4 at the cell surface, and enhanced glucose
            transport. Phosphorylation of this protein by AKT is required for
            proper translocation of GLUT4 to the cell surface. Individuals
            homozygous for a mutation in this gene are at higher risk for type
            2 diabetes and have higher levels of circulating glucose and
            insulin levels after glucose ingestion. Alternative splicing
            results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377636.8/ ENSP00000366863.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AL139230.25        125579-125579       c
            2-140               AK307493.1         1-139
            141-141             AL139230.25        125439-125439       c
            142-402             AB011175.1         87-347
            403-2331            FM207106.1         1-1929
            2332-2856           AB011175.1         2277-2801
            2857-4299           FM207106.1         2266-3708
            4300-6160           AL162571.9         86551-88411         c
            6161-6427           CA448893.1         18-284              c
            6428-7588           AL162571.9         85123-86283         c
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q22.2"
     gene            1..7588
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /note="TBC1 domain family member 4"
                     /db_xref="GeneID:9882"
                     /db_xref="HGNC:HGNC:19165"
                     /db_xref="MIM:612465"
     exon            1..900
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     CDS             403..4299
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /note="isoform 1 is encoded by transcript variant 1; TBC
                     (Tre-2, BUB2, CDC16) domain-containing protein; akt
                     substrate of 160 kDa"
                     /codon_start=1
                     /product="TBC1 domain family member 4 isoform 1"
                     /protein_id="NP_055647.2"
                     /db_xref="CCDS:CCDS41901.1"
                     /db_xref="GeneID:9882"
                     /db_xref="HGNC:HGNC:19165"
                     /db_xref="MIM:612465"
                     /translation="MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGS
                     CLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGAS
                     GGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFR
                     ATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAP
                     SSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDT
                     HLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQ
                     PRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGI
                     KHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQI
                     KLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQ
                     EENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRS
                     LTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQ
                     TFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSS
                     VRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASES
                     SDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLD
                     RNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRM
                     EKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTL
                     LKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGR
                     TFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEM
                     LKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWF
                     LTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKN
                     TLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLER
                     ANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTV
                     EQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP"
     exon            901..1482
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1572
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1677
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1810
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1902
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2013
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2133
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2208
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2435
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2600
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2624
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2785
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2995
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3154
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3313
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            3314..3558
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            3559..3718
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            3719..3888
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            3889..4065
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     exon            4066..7588
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6396..6401
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /note="hexamer: AATAAA"
     polyA_site      6418
                     /gene="TBC1D4"
                     /gene_synonym="AS160; NIDDM5"
                     /note="major polyA site"
ORIGIN      
        1 agtgtctgcc ccaggcgagc gcggcggccg cagcggggct gcagcgctgg agccggcggc
       61 cgcggggacc ctcggcgtgg tcctctgacc ctgcaaaccc gcgacggagg aaggggaggt
      121 cctgcccgag gcgccagccc aaggaggagg atgcccattt aacccgccct cgcctgccgg
      181 gcgcttgcct cggtgcccgc cgccggagcc tccgagccgc gcccgtggaa gtgctgcatg
      241 gggcagggct gctgaagcgc ggagttcggg gtcgcgccgc tcccaggcag gcgcgggagc
      301 ccggtgcggc agttggcaca gtttcggcgg cgccttctgc gcgggagtgg ggggcgcggt
      361 gcgcccggcc ggcctccgcg gtgccctggt gaggcgagag ttatggagcc gcccagctgc
      421 attcaggatg agccgttccc gcaccccctg gagcccgagc cgggcgtctc agctcagccc
      481 ggccccggga agccaagcga taagcggttc cggctgtggt acgttggggg gtcgtgcctg
      541 gaccacagga ccacgctgcc tatgctgccc tggctcatgg ccgagatccg caggcgcagc
      601 cagaagcccg aggcgggcgg ctgcggggcg ccggcggccc gagaggtgat cctggtgctc
      661 agcgcgccct tcctgcgttg cgtccccgcg ccgggcgctg gggcctcggg gggcactagt
      721 ccgtcggcca cgcagcccaa cccggcggta ttcatcttcg agcacaaggc gcagcatatc
      781 tcgcgcttca tccacaacag ccacgacctc acctactttg cctacctgat caaggcgcag
      841 cccgacgacc ccgagtcgca gatggcctgc cacgttttcc gcgccacaga ccccagccag
      901 gttcctgatg ttattagcag cataaggcaa ttatctaaag cggccatgaa agaggatgcc
      961 aaacccagca aagataatga ggacgccttt tacaactctc agaagttcga agtcctgtac
     1021 tgtggaaagg tgaccgtgac ccacaagaag gccccctcaa gcctcatcga tgactgcatg
     1081 gagaagttca gcctgcacga acagcagcgc ctgaagatcc aaggggagca gcgcggtccg
     1141 gacccaggag aggacctggc tgacttggag gtggtggtgc ccgggtcccc cggagactgc
     1201 ctgccggagg aggctgacgg caccgacacc caccttggct tacctgccgg ggccagccag
     1261 cctgccctga ccagctctcg ggtctgcttc cctgagcgga ttttggaaga ttctggcttt
     1321 gatgagcagc aggagtttcg gtctcggtgc agcagtgtca ccggcgtgca acggagagtt
     1381 cacgagggca gccagaaatc ccagccgcga cggagacacg cgagcgcacc cagtcacgtc
     1441 cagccctcgg actcggagaa gaacaggacc atgctcttcc aggttgggcg atttgagatt
     1501 aaccttatca gtccagacac taaatcagtt gtgctagaaa agaattttaa agatatctcc
     1561 tcttgttctc agggtataaa gcatgtggat cactttggct ttatctgccg ggagtctcca
     1621 gagcctggac ttagccagta tatttgttat gtattccagt gtgccagcga atctctggtt
     1681 gatgaggtaa tgctgactct gaaacaggcc ttcagtacgg cggctgccct gcagagtgcc
     1741 aagacgcaga ttaaactgtg tgaggcctgc ccgatgcact ctttgcataa gctctgtgaa
     1801 aggattgaag gtctctaccc accaagagcc aagctggtga tacagaggca tctctcatca
     1861 ctgacagata atgagcaagc tgacatcttt gaaagagttc agaaaatgaa gccagtcagt
     1921 gaccaggaag aaaatgaact tgtgatttta cacctgaggc agctgtgtga agccaagcag
     1981 aagacacacg tgcacatcgg ggaaggccct tctactattt caaatagtac aatcccagaa
     2041 aatgcaacaa gcagtggaag gttcaaactt gacattctga aaaataaagc taagagatcc
     2101 ttaactagct ccctggaaaa tatcttctca aggggagcta acagaatgag aggtcggctt
     2161 ggaagtgtgg acagttttga acggtccaac agtcttgctt cagagaagga ctactcacca
     2221 ggggattctc caccagggac accgccagcg tccccaccgt cctcagcttg gcaaacgttt
     2281 cccgaagagg attccgactc cccgcagttt cgaagacggg cacacacgtt cagccaccca
     2341 ccttcaagca caaagagaaa gctgaatttg caggatggga gggctcaggg tgtgcgttcc
     2401 cctctgctga ggcagagctc cagtgaacag tgcagcaatc tttcgtcagt tcgacgcatg
     2461 tacaaggaga gtaattcttc ctccagtctt ccaagtcttc acacttcctt ctctgcccct
     2521 tccttcactg ccccctcttt cctgaaaagc ttttaccaga attcaggtag actgtcccca
     2581 cagtatgaaa atgaaatcag acaagacact gcttcagaat caagtgatgg agaagggaga
     2641 aaaaggacct catctacctg cagcaatgag tccctaagtg tgggaggaac ctctgtcact
     2701 cctcgccgga tctcctggcg gcagcgcatt ttcctcaggg ttgcttctcc catgaacaaa
     2761 tctccctcag caatgcaaca gcaagatgga ttggacagga acgagctgct gccactgtcc
     2821 cccctctctc caaccatgga ggaggaaccg ctggttgtat tcctgtctgg ggaggatgac
     2881 ccagaaaaga ttgaagaaag aaagaaatca aaagaactga ggagcttgtg gagaaaagct
     2941 atacaccaac aaatcttgtt acttcgaatg gaaaaagaaa accagaaact tgaagcaagc
     3001 agagatgaac tccagtccag aaaagttaaa ttagactatg aagaagttgg tgcatgtcag
     3061 aaagaggtct taataacttg ggataagaag ttgttaaact gcagagctaa aatcagatgt
     3121 gatatggaag atattcatac tcttcttaaa gaaggagttc ccaaaagtcg acgaggagaa
     3181 atttggcagt ttctggcttt acagtaccga ctcagacaca gattgcctaa taaacaacag
     3241 cctcctgaca tatcctataa ggaacttttg aagcagctca ctgctcagca gcatgcgatt
     3301 ctcgtggatt taggaaggac gtttcctact cacccttact tttcagtaca gcttgggcca
     3361 ggacagctgt cactgtttaa cctcctgaaa gcctattctt tgctggacaa agaagtggga
     3421 tactgtcagg ggatcagctt tgtggctgga gtcctgcttc tgcacatgag tgaagagcaa
     3481 gcctttgaaa tgctgaaatt cctcatgtat gacctcggct tccgcaagca gtacagacct
     3541 gacatgatgt cgctgcagat tcaaatgtac cagctgtcca ggctccttca tgactatcac
     3601 agagatctct acaatcacct tgaagaaaat gaaatcagcc ccagtcttta tgctgccccc
     3661 tggttcctca cattgtttgc ctctcagttt tcattaggat ttgtagccag agtttttgat
     3721 attatttttc ttcagggaac tgaagttata ttcaaggttg cactcagcct actgagcagc
     3781 caagagacac ttataatgga atgtgagagc tttgaaaata ttgttgagtt tcttaaaaac
     3841 acgctacctg atatgaatac ctctgaaatg gaaaaaatta ttacccaggt ttttgagatg
     3901 gatatttcta agcagttgca tgcctatgag gtggaatatc atgtgctaca ggatgagctt
     3961 caggaatctt catattcctg tgaggatagt gaaactttgg agaagctgga gagggccaat
     4021 agccaactga aaagacaaaa catggacctc ctagaaaaat tacaggtagc tcatactaaa
     4081 atccaggcct tggaatcaaa cctggaaaat cttttgacga gagagaccaa aatgaagtct
     4141 ttaatccgga ccctggaaca agaaaaaatg gcttatcaaa agacagtgga gcaactccgg
     4201 aagctgctgc ccgcggatgc tctagtcaat tgtgacctgt tgctgagaga cctaaactgc
     4261 aaccctaaca acaaagccaa gataggaaat aagccataat tgaagaggca cggcctcagc
     4321 agaaagtgct ccttagaata ctacagagag gaagagcctg catgtcgctg gcccaaggct
     4381 ggaccctgaa gctgatggaa ccacctaata ctggtgctga gcgcctagtc acagcaggtg
     4441 gacctcgtgc tcatcagagc atgccaatcc taagccattg gacatatgta gactggtttt
     4501 tgttgttgct atgtacatat aaatatatat ataaaatgaa catagttcat gctttcagat
     4561 aaaatgagta gatgtatatt tagattaatt tttttagtca gaacttcatg aaatccacac
     4621 caaaggaaag gtaaactgaa atttcccttg gacatatgtg aaatcttttt gtctttatag
     4681 tgaaacaaag ccagagcatc tttgtatatt gcaatatact tgaaaaaaat gaatgtattt
     4741 ttttctccaa agaacagcat gtttcactca atggtgaaaa ggtggaaaca tttatgtaac
     4801 tttatgtgta tctgtcttga tatctactga cattgtctat atgaggaaaa tgattactgg
     4861 tcatgctcct gtgagttttt tgggaaggta gggtcatttc tccctgcctg ctttgtgcca
     4921 actagcatgt tgcatctaca tgcattatga gtctggttag gcattacttt aaacatacat
     4981 aaagagacag taggacattg tggctgagtc tacccagctc aaggtaaagg agaatgttgc
     5041 taatttttta gcaaactaga ccagcattat tactcaaact aaaaatatca cacctgaaaa
     5101 atttaattta ggacctaaaa tgtctagatt agctttctgc tttttttatt tgaataactc
     5161 attcagttgt gaatgaattc ctctttattt ggtgccacag tcaccaaatg acaaggattt
     5221 gccactttcc caccaaattg tgagtgcttg taatttaggt ctctctacct taaattcagt
     5281 ataaggaaac gtaattatga ttgatttttt ccaaagatga caagctgtgt tgaaatacat
     5341 tttttctttt gaccaattga cagaatctaa taagctttaa taatcttccc cttttatgtg
     5401 aaaagttttg agaactgtga aatgtttagg aacaaactgt tgaaatccat tggaagggaa
     5461 aaaagaaagt ggtaccagtg ttaccagctc aactaaaacc tgcaattctg catttcaact
     5521 cttcacttcc tcagcctaca aatagctcat tagatgacat tcacgcatgc tgggtatagg
     5581 caaggaaagt aattttcaaa gtacatttgc agttctcttt ttcagagatg attctatgat
     5641 agtgcctctg aaagttgatg cagcattttt gcctttccaa aaagtattta tcctcactgc
     5701 tttttgcagt acttgtattt tcacagatgg attatctggg gtaattttct tcaaagggag
     5761 tttgttatac acagtgaaaa tgtattatag agtagaatag taaagctcta ggggtttcag
     5821 aaagctttga tgaacagatg acaaacatct gaaaccccct ccgcactgtt acccagtgtg
     5881 tatataatga cttgttatag ctcagtgtgc ccttgaatcc atacagtttc ttaaaagaca
     5941 ataaaatctt attaataaag ttaatgtaac ttctaagttc tagaaaatgc tgattctgtc
     6001 tgccccattc aattgggggc tactaattga tttgttgctt ggatttcctg agaatttctc
     6061 tatttgtagg aggggttttt tctttttacg gtctgttgat gacaattact ttatgggtgt
     6121 gatgcaccga tggtagccaa ggaatctgtt ggggaagttc ggaaagaaac cttttctttc
     6181 ttttattcag tttaaagtaa actttatcct ggatgtttag aatcaacatt aagagttata
     6241 ttatggtgtt cagagattaa gctgacttgg atacaatatt ttcttttgaa aatgaatttt
     6301 ctttttcatt tgtgattttt aaaaaatgtt gcaccagtta tgcttcatgc atcgttacat
     6361 cttcatcagg ttaatgtaat gtctagttcc tttgcaataa atatattgct gcagctttct
     6421 tgacgtatac tgttactttt cgggagagaa gcagctcttg gttagagtct tttactgcag
     6481 gggtccctgg gtacctgtct gtggcctgtt atgaaccagg ctgcacagca gtggtgggca
     6541 tgcgagcaag catgactgcc tgagctctga ctcctgtcag atcagccacg gcattagatt
     6601 ctcataggag cgcaaaccct attgtgaact gtgcatgcag gagatctatg ttttgtgttc
     6661 cttaagagaa tcgaatgcct gatgatctgt cactgtctcc catcatcccc agatgggatc
     6721 gtctagttgc aggaaaacag tcagggttcc cactaattct acattatggt gagtatataa
     6781 taataatatg gtgaggatat aataataata atagaaataa agtgcacaat aaatgtgctt
     6841 gaatcattga atcatcctga aaccatcccc tgccaccagt ctgtggaaag attgtcttcc
     6901 atgaaaccag tccctggtgt caagaaggtt gaagaccgct gctttacaag atctgcatgg
     6961 tgaaacccca tctctaggga aaatacaaaa attagccagg cgtggtggtg tgcatctgta
     7021 atctcagcta ctcaggagag taggagaggc atatattgct acttatagga ggctggagaa
     7081 tagtttgaac ctgggaggcg gagcttgcag tgagcttgca gtgagccatc atgccactgc
     7141 actctagcct gggccacaga gtgagacttt gacaaaaaaa aagaaaaaca aacaaccaaa
     7201 caaaaaagat ctgcatggtt tggattgtac ctacttaaaa ttgctacaca aataattctt
     7261 cctaatagag tcagacaaca aagataattt aggagacatt tatgtaagta cattgttacc
     7321 atttttcaac ttactttctc aatggagact tttgaaagaa aaatataagc aaccacattg
     7381 acagatttgc attctaccct tttattttcc aggccccctc atagatagca agggagagag
     7441 agccacaaga aaatacaaaa ttttccaact ggacgcagat aattaagaat taaggacatt
     7501 tactgatgga tgtgatatga atgtttttat ataaaatatg catctcttgg agtattctca
     7561 tgtatatttg taatccaata aaaataca
//
