LOCUS       NM_152730               5101 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens TBC1 domain family member 32 (TBC1D32), transcript
            variant 1, mRNA.
ACCESSION   NM_152730
VERSION     NM_152730.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Bocquet,B., Borday,C., Erkilic,N., Mamaeva,D., Donval,A.,
            Masson,C., Parain,K., Kaminska,K., Quinodoz,M., Perea-Romero,I.,
            Garcia-Garcia,G., Jimenez-Medina,C., Boukhaddaoui,H., Coget,A.,
            Leboucq,N., Calzetti,G., Gandolfi,S., Percesepe,A., Barili,V.,
            Uliana,V., Delsante,M., Bozzetti,F., Scholl,H.P., Corton,M.,
            Ayuso,C., Millan,J.M., Rivolta,C., Meunier,I., Perron,M. and
            Kalatzis,V.
  TITLE     TBC1D32 variants disrupt retinal ciliogenesis and cause retinitis
            pigmentosa
  JOURNAL   JCI Insight 8 (21), e169426 (2023)
   PUBMED   37768732
  REMARK    GeneRIF: TBC1D32 variants disrupt retinal ciliogenesis and cause
            retinitis pigmentosa.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Satoda,Y., Noguchi,T., Fujii,T., Taniguchi,A., Katoh,Y. and
            Nakayama,K.
  TITLE     BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36
            contributes to intraflagellar transport turnaround involving
            ICK/CILK1
  JOURNAL   Mol Biol Cell 33 (9), ar79 (2022)
   PUBMED   35609210
  REMARK    GeneRIF: BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36
            contributes to intraflagellar transport turnaround involving
            ICK/CILK1.
REFERENCE   3  (bases 1 to 5101)
  AUTHORS   Alsahan,N. and Alkuraya,F.S.
  TITLE     Confirming TBC1D32-related ciliopathy in humans
  JOURNAL   Am J Med Genet A 182 (8), 1985-1987 (2020)
   PUBMED   32573025
  REMARK    GeneRIF: Confirming TBC1D32-related ciliopathy in humans.
REFERENCE   4  (bases 1 to 5101)
  AUTHORS   Hietamaki,J., Gregory,L.C., Ayoub,S., Iivonen,A.P., Vaaralahti,K.,
            Liu,X., Brandstack,N., Buckton,A.J., Laine,T., Kansakoski,J.,
            Hero,M., Miettinen,P.J., Varjosalo,M., Wakeling,E., Dattani,M.T.
            and Raivio,T.
  TITLE     Loss-of-Function Variants in TBC1D32 Underlie Syndromic
            Hypopituitarism
  JOURNAL   J Clin Endocrinol Metab 105 (6), 1748-1758 (2020)
   PUBMED   32060556
  REMARK    GeneRIF: Loss-of-Function Variants in TBC1D32 Underlie Syndromic
            Hypopituitarism.
REFERENCE   5  (bases 1 to 5101)
  AUTHORS   Shaheen,R., Jiang,N., Alzahrani,F., Ewida,N., Al-Sheddi,T.,
            Alobeid,E., Musaev,D., Stanley,V., Hashem,M., Ibrahim,N.,
            Abdulwahab,F., Alshenqiti,A., Sonmez,F.M., Saqati,N., Alzaidan,H.,
            Al-Qattan,M.M., Al-Mohanna,F., Gleeson,J.G. and Alkuraya,F.S.
  TITLE     Bi-allelic Mutations in FAM149B1 Cause Abnormal Primary Cilium and
            a Range of Ciliopathy Phenotypes in Humans
  JOURNAL   Am J Hum Genet 104 (4), 731-737 (2019)
   PUBMED   30905400
REFERENCE   6  (bases 1 to 5101)
  AUTHORS   Adly,N., Alhashem,A., Ammari,A. and Alkuraya,F.S.
  TITLE     Ciliary genes TBC1D32/C6orf170 and SCLT1 are mutated in patients
            with OFD type IX
  JOURNAL   Hum Mutat 35 (1), 36-40 (2014)
   PUBMED   24285566
  REMARK    GeneRIF: study identified 2 cases with a severe ciliopathy
            phenotype consistent with oro-facio-digital syndrome type IX; the
            autozygome of each index harbored a single truncating variant and
            the affected genes (SCLT1 and TBC1D32/C6orf170) have roles in
            centrosomal biology and ciliogenesis; findings suggest a role of
            SCLT1 and TBC1D32 in ciliopathy pathogenesis
REFERENCE   7  (bases 1 to 5101)
  AUTHORS   Cirulli,E.T., Kasperaviciute,D., Attix,D.K., Need,A.C., Ge,D.,
            Gibson,G. and Goldstein,D.B.
  TITLE     Common genetic variation and performance on standardized cognitive
            tests
  JOURNAL   Eur J Hum Genet 18 (7), 815-820 (2010)
   PUBMED   20125193
  REMARK    Erratum:[Eur J Hum Genet. 2010 Jul;18(7):820]
REFERENCE   8  (bases 1 to 5101)
  AUTHORS   Ko,H.W., Norman,R.X., Tran,J., Fuller,K.P., Fukuda,M. and
            Eggenschwiler,J.T.
  TITLE     Broad-minded links cell cycle-related kinase to cilia assembly and
            hedgehog signal transduction
  JOURNAL   Dev Cell 18 (2), 237-247 (2010)
   PUBMED   20159594
  REMARK    GeneRIF: Studies in mouse and zebrafish implicate that TBC1D32 is
            involved in sonic hedgehog signaling.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK091554.1, AK131446.1,
            AL589910.9, AK055461.1, AK126259.1, AL035593.11 and BX112581.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_152730.5.
            
            Summary: This gene encodes a TBC-domain containing protein. Studies
            of a similar protein in mouse and zebrafish suggest that the
            encoded protein is involved in sonic hedgehog signaling, and that
            it interacts with and stabilizes cell cycle-related kinase.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the supported protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000398212.7/ ENSP00000381270.2
            RefSeq Select criteria    :: based on computational evidence
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-477               AK091554.1         1-477
            478-694             AK091554.1         479-695
            695-1173            AK131446.1         713-1191
            1174-1174           AL589910.9         32111-32111         c
            1175-1532           AK131446.1         1193-1550
            1533-2765           AK091554.1         1534-2766
            2766-4026           AK055461.1         731-1991
            4027-4379           AK126259.1         1616-1968
            4380-4567           AK126259.1         1971-2158
            4568-4675           AL035593.11        26196-26303
            4676-5101           BX112581.1         18-443
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.31"
     gene            1..5101
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /note="TBC1 domain family member 32"
                     /db_xref="GeneID:221322"
                     /db_xref="HGNC:HGNC:21485"
                     /db_xref="MIM:615867"
     exon            1..205
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..3824
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein broad-minded; broad-minded homolog"
                     /codon_start=1
                     /product="protein broad-minded isoform 1"
                     /protein_id="NP_689943.4"
                     /db_xref="CCDS:CCDS43501.1"
                     /db_xref="GeneID:221322"
                     /db_xref="HGNC:HGNC:21485"
                     /db_xref="MIM:615867"
                     /translation="MAHFSSEDQAMLQAMLRRLFQSVKEKITGAPSLECAEEILLHLE
                     ETDENFHNYEFVKYLRQHIGNTLGSMIEEEMEKCTSDRNQGEECGYDTVVQQVTKRTQ
                     ESKEYKEMMHYLKNIMIAVVESMINKFEEDETRNQERQKKIQKEKSHSYRTDNCSDSD
                     SSLNQSYKFCQGKLQLILDQLDPGQPKEVRYEALQTLCSAPPSDVLNCENWTTLCEKL
                     TVSLSDPDPVFSDRILKFCAQTFLLSPLHMTKEIYTSLAKYLESYFLSRENHIPTLSA
                     GVDITNPNMTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLTVKHNQSHV
                     VSQKILDPIYFFALVDTKAVWFKKWMHAHYSRTTVLRLLETKYKSLVTTAIQQCVQYF
                     EMCKTRKADETLGHSKHCRNKQKTFYYLGQELQYIYFIHSLCLLGRLLIYKQGRKLFP
                     IKLKNKKGLVSLIDLLVLFTQLIYYSPSCPKMTSAAHSENYSPASMVTEVLWILSDQK
                     ECAVECLYNNIVIETLLQPIHNLMKGNEASPNCSETALIHIAGILARIASVEEGLILL
                     LYGANMNSSEESPTGAHIIAQFSKKLLDEDISIFSGSEMLPVVKGAFISVCRHIYSTC
                     EGLQVLITYNLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQESQNIMAWEDNLLD
                     DLLHFAATPKGLLLLQRTGAINECVTFIFNRYAKKLQVSRHKKFGYGVLVTRVASTAA
                     GGIALKKSGFINELITELWSNLEYGRDDVRVTHPRTTPVDPIDRSCQKSFLALVNLLS
                     YPAIYELVRNQDLPNKTEYSLREVPTCVIDIIDRLIILNSEAKIRSLFNYEQSHIFGL
                     RDFIIDGLSVERNHVLVRINLVGGPLERILPPRLLEKSDNPYPWPMFSSYPLPNCYLS
                     DITRNAGIKQDNDLDKLLLCLKISDKQTEWIENCQRQFCKMMKAKPDIISGEALIELL
                     EKFVLHLTESPSECYFPSVEYTATDANVKNESLSSVQQLGIKMTVRYGKFLSLLKDGA
                     ENDLTWVLKHCERFLKQQQTSIKSSLLCLQGNYAGHDWFVSSLFMIMLGDKEKTFQFL
                     HQFSRLLTSAFLWLPRLHISSYLPNDTVESGIHPVYFCSTHYIEMLLKAELPLVFSAF
                     HMSGFAPSQICLQWITQCFWNYLDWIEICHYIATCVFLGPDYQVYICIAVFKHLQQDI
                     LQHTQTQDLQVFLKEEALHGFRVSDYFEYMEILEQNYRTVLLRDMRNIRLQST"
     exon            206..367
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            368..545
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            546..614
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            615..740
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            741..819
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            820..923
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            924..985
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1130
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1190
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1281
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1422
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1515
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1658
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1783
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1985
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2068
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2207
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2295
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2414
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2531
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2620
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2729
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2823
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2949
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3033
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            3034..3103
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            3104..3219
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            3220..3374
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            3375..3515
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            3516..3704
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     exon            3705..5101
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5080..5085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /note="hexamer: ATTAAA"
     polyA_site      5101
                     /gene="TBC1D32"
                     /gene_synonym="BROMI; C6orf170; C6orf171"
                     /note="major polyA site"
ORIGIN      
        1 gcttttgctc ggacctggag taatgagagt ggacgtttga ttccaacagg atggcccatt
       61 tctccagcga ggaccaggcg atgctgcagg cgatgctgag gcggttgttc cagagcgtga
      121 aggagaaaat cacgggtgcc ccttccctgg agtgtgccga agagattctt ttacatctgg
      181 aggaaactga tgaaaatttt cacaactatg aatttgtgaa atacctcagg cagcatatag
      241 gcaacacttt gggttctatg attgaagaag aaatggaaaa atgcacatct gatcggaatc
      301 agggtgaaga atgcggctat gatacagttg tacagcaggt cactaaaaga actcaagaat
      361 ctaaagagta caaagaaatg atgcattacc tgaagaacat tatgatagct gtggtcgagt
      421 ctatgattaa caagtttgaa gaagatgaga cacgaaatca agaaaggcag aaaaaaatcc
      481 aaaaggagaa aagccatagt taccgcacag acaattgctc tgatagtgat tcatcattga
      541 atcagagtta caaattttgt caaggaaaat tacaattgat tttagaccag ttggatcctg
      601 ggcaacctaa agaggtgaga tatgaagcct tgcaaacatt atgttcagct cctccatctg
      661 atgtcctcaa ctgtgaaaat tggactactc tctgcgaaaa actgaccgtg tctctttcag
      721 atcctgatcc tgtgtttagt gaccggattt taaaattctg tgcacagaca tttttgcttt
      781 ctccattaca tatgaccaag gaaatttata caagcttagc taagtatttg gagtcatact
      841 ttctttctag ggaaaatcat attcctactc tttcagctgg tgtagatata actaatccta
      901 atatgactcg cttacttaaa aaggttcgtc ttctaaatga atatcagaaa gaagctccat
      961 ctttctggat tcgtcatcca gagaagtata tggaagaaat tgtggagagt actttgtcct
     1021 tgttaacagt taaacataat caaagccatg ttgtctcaca aaagattttg gatccgatct
     1081 acttttttgc attagttgat accaaggctg tgtggttcaa aaagtggatg catgcacatt
     1141 atagcagaac tacagtatta agacttcttg aaacgaaata taagtctctg gtaactacag
     1201 ccattcaaca gtgtgttcag tactttgaaa tgtgtaagac taggaaagct gatgaaactt
     1261 tgggacattc aaagcattgc agaaacaagc agaaaacttt ctactactta ggacaagaat
     1321 tacaatatat ttatttcatt cactcactgt gccttttagg aagattattg atctataaac
     1381 aaggcagaaa actatttcct attaagctga agaataaaaa aggtttggta tccctcatag
     1441 atctgcttgt tctttttacc caacttatct attactcacc aagttgtcca aagatgacat
     1501 cagctgccca ttcagagaat tactctcctg caagtatggt gactgaagtt ctgtggatac
     1561 tcagtgatca aaaagaatgt gcagtggaat gcttatataa caacattgta atagagacac
     1621 ttcttcagcc tattcacaat ttaatgaaag gaaatgaggc atctccaaat tgctctgaga
     1681 cagctttaat tcatatagct ggtattttgg caagaattgc atctgtagaa gaagggctta
     1741 ttttactcct ttatggagca aatatgaact cttctgaaga aagtcctaca ggtgctcata
     1801 taattgccca gttttcgaaa aaacttctcg atgaagatat ttctatattt tctggatcag
     1861 aaatgttgcc tgtggttaaa ggagctttta tttctgtgtg tcgtcacata tatagtacat
     1921 gtgaaggttt gcaggtgtta atcacttata atttgcatga atctatagca aaggcatgga
     1981 aaaagacaag tttgctatca gaaagaattc ctactccagt agagggttct gattctgttt
     2041 cttcagtaag ccaggaatcc caaaacatta tggcttggga agataatttg ttagatgatt
     2101 tactacattt tgctgccacc cccaaaggat tactacttct tcaaagaaca ggtgctatca
     2161 atgaatgtgt gacatttata ttcaatcgat atgcaaaaaa attacaggtc agcaggcata
     2221 aaaaatttgg ctatggagtt ttggttacac gagtggcatc aacagcagca ggtggcattg
     2281 cactaaaaaa gtcagggttt attaatgaac ttataactga attatggtcc aatctggaat
     2341 atggaagaga tgatgttagg gtaacccatc ccagaactac tccagtggat cctattgacc
     2401 gaagctgtca aaagtctttt ttagcactgg tgaacttgtt atcctatcct gctatttatg
     2461 agcttgtaag gaatcaagat cttcctaata aaacagaata ttctcttcgt gaagtcccaa
     2521 catgtgttat tgatattatt gatagactta taattttgaa ttctgaagct aagattcgtt
     2581 ctttattcaa ctatgaacaa tcacatatct ttggtctaag ggactttata attgatggct
     2641 tatcagtgga gagaaatcat gttcttgtta gaataaatct tgttggtggg ccattggaac
     2701 ggattttgcc tccgaggtta ctcgaaaaga gtgataatcc atatccttgg ccaatgtttt
     2761 catcatatcc attgccaaac tgctatctgt cagacattac aagaaatgct ggtataaaac
     2821 aagacaatga tcttgacaag cttttattat gcctcaaaat atctgataaa caaactgaat
     2881 ggatagaaaa ctgccaaaga caattttgca aaatgatgaa agccaaacct gatataatca
     2941 gtggagaggc cttaatagaa ttacttgaaa aatttgtgct tcatctcact gaaagcccat
     3001 ctgaatgcta cttcccttca gtggagtata cagctactga tgcaaatgtg aagaatgaaa
     3061 gtctttcatc tgtgcagcag cttggcatta aaatgactgt caggtatggc aaattcctca
     3121 gtctcttaaa agatggtgca gaaaatgatc ttacctgggt tttaaagcat tgtgagagat
     3181 tcctgaaaca gcagcaaact tccataaaat cttctcttct ctgcctgcaa gggaattatg
     3241 ctggccatga ctggtttgta tcttctctgt tcatgataat gttgggagac aaagaaaaaa
     3301 cattccaatt tcttcatcaa ttctccaggc ttctgacttc tgcttttctt tggttgccaa
     3361 ggctacatat ttctagttac cttcctaatg acactgtaga atctggcatc catccagtat
     3421 atttttgcag cacccattat attgaaatgc tactgaaggc tgagttgcct cttgtgtttt
     3481 cagcttttca catgtctggt tttgcaccat cacagatttg cctgcaatgg ataacccagt
     3541 gtttttggaa ttacttagat tggatagaaa tctgccatta tattgctact tgtgttttcc
     3601 ttggtcctga ttatcaagtg tatatctgta tagctgtatt caaacattta cagcaagaca
     3661 ttctacagca cactcagact caagatctgc aagttttcct aaaagaagaa gcactgcatg
     3721 ggtttcgagt gagtgattat tttgaataca tggaaatttt ggaacaaaac taccgaacag
     3781 tgctgctgag agacatgcgg aacattagac tgcagagcac atagatcatg agacacacgg
     3841 tttaaattta ggttttattt atttttaaac acagcagggg ggcttgatgt ttttctgtgt
     3901 ctgtaacaac atttactttg tgaatataca tattgtaaat actgagaagt ataacgatat
     3961 atttaagtag gtatgagctc aatttgtgaa ttcatttttg taaatttgtt gttttgtaag
     4021 gttattatag aatcagatct agcttacttt tagttcttat tcatgtttaa gagttagtcc
     4081 tggccaggcg cggtggctca tgcctgtaat cccagcactt tgggagtctg aggtgggcgg
     4141 atcacgaggt caagagatcg agaccatcct ggccaaaatg gtgaaacctc gtctctgcta
     4201 acaatactga aattagctgg gtgcagtgat gcgcctgtag tccctgctac ttgggaggct
     4261 gaggcaggag aatcgcttga acccgggagg cggaggttgc agtgagccaa gattgtgcca
     4321 ctgtactcca gccaggccac agagtgagac tctgtctcaa aaaaaaaaaa aaaaaaaaag
     4381 tcccaactta catctccttt attcagatga tttaaatatt gtttccagtg aatttggaaa
     4441 ggagaagtaa tagtgtaaat aatattttga ctagctgcag aaagcccata agacaaggaa
     4501 aagacagtat ttcttccatt ctttatgtct gtacatgtaa aggaaaatgg ataaaactac
     4561 agctgcttct tttacatgtg gaagaacaat gatactattt accatggcaa gtggtaggaa
     4621 aactgttgtc cttggacata attgtttttt aggagttgct tttgataccc atatcaattt
     4681 ataattcttt gtttgaaatg aagtctttac atggttcatt gaagagatag attggttatt
     4741 tcatactgat aagcattcta ctcttatttg ttatgcattt tccttagtga tatattttac
     4801 ttgtactgaa cttgaaaata taaaggagaa tacatttcta aattatttta aatggctaac
     4861 actatgattt gtcttattta aatagatgtc tctgcaccgg taagattaat acaatatgtg
     4921 aatgtctatt ttttatatct taactcacaa tgagtatatg aaagataata cacgaatata
     4981 ttacattatt catttttagt catgagttta tttcaataag tttttctaat tgtagatact
     5041 gttttttatt ctttccttgt atctaaatat aaatcaacca ttaaaatcat tctaactcta
     5101 a
//
