LOCUS       NM_001146213            3157 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens TBC1 domain family member 15 (TBC1D15), transcript
            variant 3, mRNA.
ACCESSION   NM_001146213
VERSION     NM_001146213.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Choi,H.Y., Zhu,Y., Zhao,X., Mehta,S., Hernandez,J.C., Lee,J.J.,
            Kou,Y., Machida,R., Giacca,M., Del Sal,G., Ray,R., Eoh,H.,
            Tahara,S.M., Chen,L., Tsukamoto,H. and Machida,K.
  TITLE     NOTCH localizes to mitochondria through the TBC1D15-FIS1
            interaction and is stabilized via blockade of E3 ligase and CDK8
            recruitment to reprogram tumor-initiating cells
  JOURNAL   Exp Mol Med 56 (2), 461-477 (2024)
   PUBMED   38409448
  REMARK    GeneRIF: NOTCH localizes to mitochondria through the TBC1D15-FIS1
            interaction and is stabilized via blockade of E3 ligase and CDK8
            recruitment to reprogram tumor-initiating cells.
            Erratum:[Exp Mol Med. 2024 Oct;56(10):2323. doi:
            10.1038/s12276-024-01325-9.. PMID: 39465353]
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Ihenacho,U.K., Toro,R., Mansour,R.H. and Hill,R.B.
  TITLE     A conserved, noncanonical insert in FIS1 mediates TBC1D15 and DRP1
            recruitment for mitochondrial fission
  JOURNAL   J Biol Chem 299 (11), 105303 (2023)
   PUBMED   37777154
  REMARK    GeneRIF: A conserved, noncanonical insert in FIS1 mediates TBC1D15
            and DRP1 recruitment for mitochondrial fission.
REFERENCE   3  (bases 1 to 3157)
  AUTHORS   Shimada,K., Park,S., Miyata,H., Yu,Z., Morohoshi,A., Oura,S.,
            Matzuk,M.M. and Ikawa,M.
  TITLE     ARMC12 regulates spatiotemporal mitochondrial dynamics during
            spermiogenesis and is required for male fertility
  JOURNAL   Proc Natl Acad Sci U S A 118 (6) (2021)
   PUBMED   33536340
REFERENCE   4  (bases 1 to 3157)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3157)
  AUTHORS   Choi,H.Y., Siddique,H.R., Zheng,M., Kou,Y., Yeh,D.W., Machida,T.,
            Chen,C.L., Uthaya Kumar,D.B., Punj,V., Winer,P., Pita,A., Sher,L.,
            Tahara,S.M., Ray,R.B., Liang,C., Chen,L., Tsukamoto,H. and
            Machida,K.
  TITLE     p53 destabilizing protein skews asymmetric division and enhances
            NOTCH activation to direct self-renewal of TICs
  JOURNAL   Nat Commun 11 (1), 3084 (2020)
   PUBMED   32555153
  REMARK    GeneRIF: p53 destabilizing protein skews asymmetric division and
            enhances NOTCH activation to direct self-renewal of TICs.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3157)
  AUTHORS   Behrends,C., Sowa,M.E., Gygi,S.P. and Harper,J.W.
  TITLE     Network organization of the human autophagy system
  JOURNAL   Nature 466 (7302), 68-76 (2010)
   PUBMED   20562859
REFERENCE   7  (bases 1 to 3157)
  AUTHORS   Peralta,E.R., Martin,B.C. and Edinger,A.L.
  TITLE     Differential effects of TBC1D15 and mammalian Vps39 on Rab7
            activation state, lysosomal morphology, and growth factor
            dependence
  JOURNAL   J Biol Chem 285 (22), 16814-16821 (2010)
   PUBMED   20363736
  REMARK    GeneRIF: although only TBC1D15/Rab7-GAP altered Rab7-GTP levels,
            both Rab7-GAP and mVps39 regulate lysosomal morphology and play a
            role in maintaining growth factor dependence
REFERENCE   8  (bases 1 to 3157)
  AUTHORS   Yoshimura,S., Egerer,J., Fuchs,E., Haas,A.K. and Barr,F.A.
  TITLE     Functional dissection of Rab GTPases involved in primary cilium
            formation
  JOURNAL   J Cell Biol 178 (3), 363-369 (2007)
   PUBMED   17646400
REFERENCE   9  (bases 1 to 3157)
  AUTHORS   Itoh,T., Satoh,M., Kanno,E. and Fukuda,M.
  TITLE     Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16)
            domain-containing proteins based on their Rab-binding activity
  JOURNAL   Genes Cells 11 (9), 1023-1037 (2006)
   PUBMED   16923123
REFERENCE   10 (bases 1 to 3157)
  AUTHORS   Zhang,X.M., Walsh,B., Mitchell,C.A. and Rowe,T.
  TITLE     TBC domain family, member 15 is a novel mammalian Rab
            GTPase-activating protein with substrate preference for Rab7
  JOURNAL   Biochem Biophys Res Commun 335 (1), 154-161 (2005)
   PUBMED   16055087
  REMARK    GeneRIF: This paper primarily describes mouse TBC1D15 but also
            reports the primary structure of human TBC1D15.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC089984.23 and AC090109.15.
            
            On Jul 29, 2020 this sequence version replaced NM_001146213.2.
            
            Summary: This gene encodes a member of the Ras-like proteins in
            brain-GTPase activating protein superfamily that share a conserved
            Tre-2/Bub2/Cdc16 domain. The encoded protein interacts with
            Ras-like protein in brain 5A and may function as a regulator of
            intracellular trafficking. Alternate splicing results in multiple
            transcript variants. [provided by RefSeq, Apr 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK022147.1, SRR18074968.1817749.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000485960.7/ ENSP00000420678.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AC089984.23        73004-73056
            54-152              AC089984.23        105315-105413
            153-227             AC089984.23        106174-106248
            228-366             AC089984.23        113714-113852
            367-577             AC089984.23        118056-118266
            578-680             AC089984.23        126467-126569
            681-878             AC089984.23        127931-128128
            879-1007            AC089984.23        129192-129320
            1008-1111           AC089984.23        129922-130025
            1112-1206           AC089984.23        131092-131186
            1207-1323           AC089984.23        140267-140383
            1324-1424           AC089984.23        147071-147171
            1425-1524           AC090109.15        8792-8891
            1525-1622           AC090109.15        9545-9642
            1623-1739           AC090109.15        11825-11941
            1740-1826           AC090109.15        12462-12548
            1827-3157           AC090109.15        14077-15407
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.1"
     gene            1..3157
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /note="TBC1 domain family member 15"
                     /db_xref="GeneID:64786"
                     /db_xref="HGNC:HGNC:25694"
                     /db_xref="MIM:612662"
     exon            1..53
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..2048
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /EC_number="1.15.1.1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     Tre2/Bub2/Cdc16 domain family member 15; Tre-2-budding
                     uninhibited by benzimidazole-cell division cycle 16
                     domain, domain family member 15; GAP for RAB7;
                     GTPase-activating protein RAB7"
                     /codon_start=1
                     /product="TBC1 domain family member 15 isoform 3"
                     /protein_id="NP_001139685.2"
                     /db_xref="CCDS:CCDS53814.1"
                     /db_xref="GeneID:64786"
                     /db_xref="HGNC:HGNC:25694"
                     /db_xref="MIM:612662"
                     /translation="MAAAGVVSGKIIYEQEGVYIHSSCGKTNDQDGLISGILRVLEKD
                     AEVIVDWRPLDDALDSSSILYARKDSSSVVEWTQAPKERGHRGSEHLNSYEAEWDMVN
                     TVSFKRKPHTNGDAPSHRNGKSKWSFLFSLTDLKSIKQNKEGMGWSYLVFCLKDDVVL
                     PALHFHQGDSKLLIESLEKYVVLCESPQDKRTLLVNCQNKSLSQSFENLLDEPAYGLI
                     QKIKKDPYTATMIGFSKVTNYIFDSLRGSDPSTHQRPPSEMADFLSDAIPGLKINQQE
                     EPGFEVITRIDLGERPVVQRREPVSLEEWTKNIDSEGRILNVDNMKQMIFRGGLSHAL
                     RKQAWKFLLGYFPWDSTKEERTQLQKQKTDEYFRMKLQWKSISQEQEKRNSRLRDYRS
                     LIEKDVNRTDRTNKFYEGQDNPGLILLHDILMTYCMYDFDLGYVQGMSDLLSPLLYVM
                     ENEVDAFWCFASYMDQMHQNFEEQMQGMKTQLIQLSTLLRLLDSGFCSYLESQDSGYL
                     YFCFRWLLIRFKREFSFLDILRLWEVMWTELPCTNFHLLLCCAILESEKQQIMEKHYG
                     FNEILKHINELSMKIDVEDILCKAEAISLQMVKCKELPQAVCEILGLQGSEVTTPDSD
                     VGEDENVVMTPCPTSAFQSNALPTLSASGARNDSPTQIPVSSDVCRLTPA"
     exon            54..152
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            153..227
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            228..366
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            367..577
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            578..680
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            681..878
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1007
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1111
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1206
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1323
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1424
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1524
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1622
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1739
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1826
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..3157
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2183..2188
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /note="hexamer: ATTAAA"
     polyA_site      2210
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /note="major polyA site"
     regulatory      3140..3145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /note="hexamer: AATAAA"
     polyA_site      3157
                     /gene="TBC1D15"
                     /gene_synonym="RAB7-GAP"
                     /note="major polyA site"
ORIGIN      
        1 attaccaggc acgcgcagga aacatggcgg cggcgggtgt tgtgagcggg aagattatat
       61 atgaacaaga aggagtatat attcactcat cttgtggaaa gaccaatgac caagacggct
      121 tgatttcagg aatattacgt gttttagaaa aggatgccga agtaatagtg gactggagac
      181 cattggatga tgcattagat tcctctagta ttctctatgc tagaaaggac tccagttcag
      241 ttgtagaatg gactcaggcc ccaaaagaaa gaggtcatcg aggatcagaa catctgaaca
      301 gttacgaagc agaatgggac atggttaata cagtttcatt taaaaggaaa ccacatacca
      361 atggagatgc tccaagtcat agaaatggga aaagcaaatg gtcattcctg ttcagtttga
      421 cagacctgaa atcaatcaag caaaacaaag agggtatggg ctggtcctat ttggtattct
      481 gtctaaagga tgacgtcgtt ctccctgctc tacactttca tcaaggagat agcaaactac
      541 tgattgaatc tcttgaaaaa tatgtggtat tgtgtgaatc tccacaggat aaaagaacac
      601 ttcttgtgaa ttgtcagaat aagagtcttt cacagtcttt tgaaaatctt cttgatgagc
      661 cagcatatgg tttaatacaa aaaattaaaa aggaccctta tacggcaact atgataggat
      721 tttccaaagt cacaaactac atttttgaca gtttgagagg cagcgatccc tctacacatc
      781 aacgaccacc ttcagaaatg gcagattttc ttagtgatgc tattccaggt ctaaagataa
      841 atcaacaaga agaaccagga tttgaagtca tcacaagaat tgatttgggg gaacgccctg
      901 ttgttcaaag gagagaaccg gtatcactgg aagaatggac taagaacatt gattctgaag
      961 gaagaatttt aaatgtagat aatatgaagc agatgatatt tagaggggga cttagtcatg
     1021 cattgagaaa gcaagcatgg aaatttcttc tgggttattt tccctgggac agtaccaagg
     1081 aggaaagaac ccaattacaa aagcaaaaaa ctgatgaata cttcagaatg aaactgcagt
     1141 ggaaatccat cagccaggaa caagagaaaa gaaattcgag gttaagagat tatagaagtc
     1201 ttatcgaaaa agatgttaac agaacagatc gaacaaacaa gttttatgaa ggccaagata
     1261 atccagggtt gattttactt catgacattt tgatgaccta ctgtatgtat gattttgatt
     1321 taggatatgt tcaaggaatg agtgatttac tttcccctct tttatatgtg atggaaaatg
     1381 aagtggatgc cttttggtgc tttgcctctt acatggacca aatgcatcag aattttgaag
     1441 aacaaatgca aggcatgaag acccagctaa ttcagctgag taccttactt cgattgttag
     1501 acagtggatt ttgcagttac ttagaatctc aggactctgg atacctttat ttttgcttca
     1561 ggtggctttt aatcagattc aaaagggaat ttagttttct agatattctt cgattatggg
     1621 aggtaatgtg gaccgaacta ccatgtacaa atttccatct tcttctctgt tgtgctattc
     1681 tggaatcaga aaagcagcaa ataatggaaa agcattatgg cttcaatgaa atacttaagc
     1741 atatcaatga attgtccatg aaaattgatg tggaagatat actctgcaag gcagaagcaa
     1801 tttctctaca gatggtaaaa tgcaaggaat tgccacaagc agtctgtgag atccttgggc
     1861 ttcaaggcag tgaagttaca acaccagatt cagacgttgg tgaagacgaa aatgttgtca
     1921 tgactccttg tcctacatct gcatttcaaa gtaatgcctt gcctacactc tctgccagtg
     1981 gagccagaaa tgacagccca acacagatac cagtgtcctc agatgtctgc agattaacac
     2041 ctgcatgatc actgttcttg cttttttggg aagagacact ttgttgcaac cctttttcaa
     2101 gtacttgaaa gttgaaaatt tgaaatcttg gtattgatca tgctttaagg tttatgtaaa
     2161 gaaagtgtac tgatgttctt acattaaagc tttacaaaga tttaaactaa ttatttttgt
     2221 agttacttct accaaatagc ctttcctttt cgataacatt cctcagtatt tttatagcca
     2281 agtacatttt attttcttgc tgatgaactg gaattggata aatattgcaa gtggatgagt
     2341 tggaaattat gcactttgaa aaacattcac tttgtttaag cttattgggt ttcagatttg
     2401 attaaattaa atgtggaggc tttctatagc attctaagct gagaagtaga ttgttaccca
     2461 gtaatgaaat aaaaaataaa aataaaagga tttttttctc tattgtttac gacagtactc
     2521 agcttaaata tttatgctgg tcaaatgtga tttaaattgg acattttcat caatgcagtc
     2581 taatgtgtag ataaatattt caaccataat aagtggattg gcagtatatt ttttacattg
     2641 aacttttctt cacttgtata taaagattat atataagtac ttatttatga gtataagaaa
     2701 ggttaggcat attttcatta actgaataaa cgacttgatt tatataacct ggtttatcaa
     2761 aatttaacat ggcttcagta tgagatcttt ttcaaaacta ttttcttaaa catttatttc
     2821 atgagattat gttcaaccct gtacctggtg taattttaaa attaattgct tgtaacctca
     2881 ctttactaat aatgtttatt atctttccta ataatgcatt aactgattaa tcaggtgttt
     2941 aaatttttat aaaatactct tgcaaaaagt ttatttgaaa aatttctaga tggtctcatg
     3001 agtttcaaaa taataatttt tgtgtatgaa caaagctgtt gtttttacca tgcagtattg
     3061 catgatttta agttatgtgg aattaacata actgattttg ttttaattgt aagttgttaa
     3121 ctcctgtata tatcattaaa ataaatctga agttgaa
//
