LOCUS       NM_017714               2342 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens taspase 1 (TASP1), transcript variant 1, mRNA.
ACCESSION   NM_017714
VERSION     NM_017714.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Oelschlager,L., Stahl,P., Kaschani,F., Stauber,R.H., Knauer,S.K.
            and Hensel,A.
  TITLE     Taspase1 Facilitates Topoisomerase IIbeta-Mediated DNA
            Double-Strand Breaks Driving Estrogen-Induced Transcription
  JOURNAL   Cells 12 (3), 363 (2023)
   PUBMED   36766705
  REMARK    GeneRIF: Taspase1 Facilitates Topoisomerase IIbeta-Mediated DNA
            Double-Strand Breaks Driving Estrogen-Induced Transcription.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Nagaratnam,N., Delker,S.L., Jernigan,R., Edwards,T.E., Snider,J.,
            Thifault,D., Williams,D., Nannenga,B.L., Stofega,M., Sambucetti,L.,
            Hsieh,J.J., Flint,A.J., Fromme,P. and Martin-Garcia,J.M.
  TITLE     Structural insights into the function of the catalytically active
            human Taspase1
  JOURNAL   Structure 29 (8), 873-885 (2021)
   PUBMED   33784495
  REMARK    GeneRIF: Structural insights into the function of the catalytically
            active human Taspase1.
REFERENCE   3  (bases 1 to 2342)
  AUTHORS   Lin,Y., Huang,Y., Liang,C., Xie,S. and Xie,A.
  TITLE     Silencing of circTASP1 inhibits proliferation and induces apoptosis
            of acute myeloid leukaemia cells through modulating
            miR-515-5p/HMGA2 axis
  JOURNAL   J Cell Mol Med 25 (15), 7367-7380 (2021)
   PUBMED   34197029
  REMARK    GeneRIF: Silencing of circTASP1 inhibits proliferation and induces
            apoptosis of acute myeloid leukaemia cells through modulating
            miR-515-5p/HMGA2 axis.
REFERENCE   4  (bases 1 to 2342)
  AUTHORS   Wan,X.M., Zhou,X.L., Du,Y.J., Shen,H., Yang,Z. and Ding,Y.
  TITLE     TASP1 Promotes Proliferation and Migration in Gastric Cancer via
            EMT and AKT/P-AKT Pathway
  JOURNAL   J Immunol Res 2021, 5521325 (2021)
   PUBMED   34012990
  REMARK    GeneRIF: TASP1 Promotes Proliferation and Migration in Gastric
            Cancer via EMT and AKT/P-AKT Pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2342)
  AUTHORS   Zhang,Y., Du,P., Li,Y., Zhu,Q., Song,X., Liu,S., Hao,J., Liu,L.,
            Liu,F., Hu,Y., Jiang,L., Ma,Q., Lu,W. and Liu,Y.
  TITLE     TASP1 Promotes Gallbladder Cancer Cell Proliferation and Metastasis
            by Up-regulating FAM49B via PI3K/AKT Pathway
  JOURNAL   Int J Biol Sci 16 (5), 739-751 (2020)
   PUBMED   32071545
  REMARK    GeneRIF: TASP1 Promotes Gallbladder Cancer Cell Proliferation and
            Metastasis by Up-regulating FAM49B via PI3K/AKT Pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2342)
  AUTHORS   Zhou,H., Spicuglia,S., Hsieh,J.J., Mitsiou,D.J., Hoiby,T.,
            Veenstra,G.J., Korsmeyer,S.J. and Stunnenberg,H.G.
  TITLE     Uncleaved TFIIA is a substrate for taspase 1 and active in
            transcription
  JOURNAL   Mol Cell Biol 26 (7), 2728-2735 (2006)
   PUBMED   16537915
  REMARK    GeneRIF: Transfected taspase 1 enhances cleavage of TFIIA, and RNA
            interference knockdown of endogenous taspase 1 diminishes cleavage
            of TFIIA in vivo.
REFERENCE   7  (bases 1 to 2342)
  AUTHORS   Khan,J.A., Dunn,B.M. and Tong,L.
  TITLE     Crystal structure of human Taspase1, a crucial protease regulating
            the function of MLL
  JOURNAL   Structure 13 (10), 1443-1452 (2005)
   PUBMED   16216576
REFERENCE   8  (bases 1 to 2342)
  AUTHORS   Hsieh,J.J., Cheng,E.H. and Korsmeyer,S.J.
  TITLE     Taspase1: a threonine aspartase required for cleavage of MLL and
            proper HOX gene expression
  JOURNAL   Cell 115 (3), 293-303 (2003)
   PUBMED   14636557
  REMARK    GeneRIF: purification and cloning of threonine aspartase 1
            responsible for cleaving MLL; RNAi-mediated knockdown of Taspase1
            results in the appearance of unprocessed MLL and the loss of proper
            HOX gene expression
REFERENCE   9  (bases 1 to 2342)
  AUTHORS   Hsieh,J.J., Ernst,P., Erdjument-Bromage,H., Tempst,P. and
            Korsmeyer,S.J.
  TITLE     Proteolytic cleavage of MLL generates a complex of N- and
            C-terminal fragments that confers protein stability and subnuclear
            localization
  JOURNAL   Mol Cell Biol 23 (1), 186-194 (2003)
   PUBMED   12482972
REFERENCE   10 (bases 1 to 2342)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL601028.1, BC025266.1 and
            CA418131.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017714.2.
            
            Summary: This gene encodes an endopeptidase that cleaves specific
            substrates following aspartate residues. The encoded protein
            undergoes posttranslational autoproteolytic processing to generate
            alpha and beta subunits, which reassemble into the active
            alpha2-beta2 heterotetramer. It is required to cleave MLL, a
            protein required for the maintenance of HOX gene expression, and
            TFIIA, a basal transcription factor. Alternatively spliced
            transcript variants have been described, but their biological
            validity has not been determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.101399.1,
                                           SRR3476690.70013.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337743.9/ ENSP00000338624.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AL601028.1         7-22
            17-1885             BC025266.1         1-1869
            1886-2342           CA418131.1         23-479              c
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.1"
     gene            1..2342
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /note="taspase 1"
                     /db_xref="GeneID:55617"
                     /db_xref="HGNC:HGNC:15859"
                     /db_xref="MIM:608270"
     exon            1..39
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            40..258
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..1376
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /EC_number="3.4.25.-"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; threonine aspartase 1"
                     /codon_start=1
                     /product="threonine aspartase 1 isoform 1 precursor"
                     /protein_id="NP_060184.2"
                     /db_xref="CCDS:CCDS13116.1"
                     /db_xref="GeneID:55617"
                     /db_xref="HGNC:HGNC:15859"
                     /db_xref="MIM:608270"
                     /translation="MTMEKGMSSGEGLPSRSSQVSAGKITAKELETKQSYKEKRGGFV
                     LVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAG
                     MGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRI
                     PPCFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKK
                     RRQSSEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAE
                     NTGAHNPYSTAVSTSGCGEHLVRTILARECSHALQAEDAHQALLETMQNKFISSPFLA
                     SEDGVLGGVIVLRSCRCSAEPDSSQNKQTLLVEFLWSHTTESMCVGYMSAQDGKAKTH
                     ISRLPPGAVAGQSVAIEGGVCRLESPVN"
     exon            259..326
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            327..395
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            396..516
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            517..601
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            602..681
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            682..788
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            789..908
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            909..987
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1098
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1209
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1283
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..2342
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2269..2274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /note="hexamer: AATAAA"
     polyA_site      2291
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /note="major polyA site"
     regulatory      2320..2325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /note="hexamer: AATAAA"
     polyA_site      2342
                     /gene="TASP1"
                     /gene_synonym="C20orf13; dJ585I14.2; SULEHS"
                     /note="major polyA site"
ORIGIN      
        1 gactacttcc ggtgcagtga aggctcgggg ctgaagcggg gtaattcctc tcctgcaatt
       61 acttttggat ggaagtatgc ccctttctca gtagaagatg gtaatcttgg agaatgacca
      121 tggagaaggg gatgagttct ggagaagggc tgccttccag atcatctcag gtttcggctg
      181 gtaaaataac agccaaagag ttggaaacaa agcagtccta taaagagaaa cgaggaggct
      241 ttgtgttggt gcatgcaggt gcaggttatc attctgaatc caaagccaag gagtataaac
      301 atgtatgcaa acgagcttgt cagaaggcaa ttgaaaagct gcaggccggt gctcttgcaa
      361 ctgacgcagt cactgcagca ctggtggaac ttgaggattc tccttttaca aatgcaggaa
      421 tgggatctaa tctaaatctg ttaggtgaaa ttgagtgtga tgccagcata atggatggaa
      481 aatccttaaa ttttggagca gttggagcac tgagtggaat caagaaccca gtctcggttg
      541 ccaacagact cttatgtgaa gggcagaagg gcaagctctc ggctggcaga attcctccct
      601 gctttttagt tggagaagga gcctacagat gggcagtaga tcatggaata ccctcttgcc
      661 ctcctaacat catgaccaca agattcagtt tagctgcatt taaaagaaac aagaggaaac
      721 tagagctggc agaaagggtg gacacagatt ttatgcaact aaagaaaaga agacaatcaa
      781 gtgagaagga aaatgactca ggcactttgg acacggtagg cgctgtggtt gtggaccacg
      841 aagggaatgt tgctgctgct gtctccagtg gaggcttggc cttgaaacat ccggggagag
      901 ttgggcaggc tgctctttat ggatgtggct gctgggctga aaatactgga gctcataacc
      961 cctactccac agctgtgagt acctcaggat gtggagagca tcttgtgcgc accatactgg
     1021 ctagagaatg ttcacatgct ttacaagctg aggatgctca ccaagccctg ttggagacta
     1081 tgcaaaacaa gtttatcagt tcacctttcc ttgccagtga agatggcgtg cttggcggag
     1141 tgattgtcct ccgttcatgc agatgttctg ccgagcctga ctcctcccaa aataagcaga
     1201 cacttctagt ggaatttctg tggagccaca cgacggagag catgtgtgtc ggatatatgt
     1261 cagcccagga tgggaaagcc aagactcaca tttcaagact tcctcctggt gcggtggcag
     1321 gacagtctgt ggcaatcgaa ggtggggtgt gccgcctgga gagcccagtg aactgaccct
     1381 tcaggctgag tgtgaagcgt ctcagaggca tttcagaacc tgagcttttg ggggttttta
     1441 actgaagttg gttgttttat ctttcttgtt ttataattcc tattgcaacc tcgtgcactg
     1501 ctcgagacac aagtgctgct gtagttagcg cttagtgaca cgcgggcctt tggtgggtga
     1561 gcgggactgt gtgtgagtgt gtgcgcgtat gtgcgcacat atgtgtatgt gtggagtatg
     1621 tgtgtttgct tctccgtgga tgaaatagaa actcctcatt gtgtgaccag gaatggttaa
     1681 atcatcttta caaaatgtgt gctttaactg tttacaagta aaacctaaag ttgcaggaaa
     1741 cattttttat ttcgtaaaga ggtaccaact gtcgctgatg tgatatgtca gaactgaaga
     1801 gtaaatctac ttgtttaaat gacttgacag tggtagtgct ccatttaata acagtaataa
     1861 gtaataaagt gtttttattt gttaaccagt ttaagtggat cctgtggtaa cttaaactgt
     1921 tgttctcatc ccttatatgg ggcatttttc tttaacaaag aatggtttca gtgaaacaat
     1981 ctagcagaga attaatgtca gaaccttttt aaataatagt ctgattgata cagtttgtac
     2041 ttatttcatc aagcttttct aagcttaaat attgcatagc ttcgagctgt atggactata
     2101 ttatgaaaga atatgtaaag agaacataca gtaatgcaca gtccttaatt tgtgtataat
     2161 ggaaagttat ttacaatata acactgtaaa taagaaagca aagtttatgg gaaaattcaa
     2221 tattatcttt gtttttgttt aaatatattt ttaagataaa ggcacaaaaa taaaagaagc
     2281 gtattactgg gtatagtatg tgactcctct tctcagacta ataaattatc ttttgaatcc
     2341 tt
//
