LOCUS       NM_001394998           12511 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens tetratricopeptide repeat, ankyrin repeat and
            coiled-coil containing 2 (TANC2), transcript variant 1, mRNA.
ACCESSION   NM_001394998 XM_006721806
VERSION     NM_001394998.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Broniarczyk,J.K., Massimi,P., Trejo-Cerro,O., Myers,M.P. and
            Banks,L.
  TITLE     HPV-18E6 Inhibits Interactions between TANC2 and SNX27 in a
            PBM-Dependent Manner and Promotes Increased Cell Proliferation
  JOURNAL   J Virol 96 (22), e0136522 (2022)
   PUBMED   36326272
  REMARK    GeneRIF: HPV-18E6 Inhibits Interactions between TANC2 and SNX27 in
            a PBM-Dependent Manner and Promotes Increased Cell Proliferation.
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Kim,S.G., Lee,S., Kim,Y., Park,J., Woo,D., Kim,D., Li,Y., Shin,W.,
            Kang,H., Yook,C., Lee,M., Kim,K., Roh,J.D., Ryu,J., Jung,H.,
            Um,S.M., Yang,E., Kim,H., Han,J., Heo,W.D. and Kim,E.
  TITLE     Tanc2-mediated mTOR inhibition balances mTORC1/2 signaling in the
            developing mouse brain and human neurons
  JOURNAL   Nat Commun 12 (1), 2695 (2021)
   PUBMED   33976205
  REMARK    GeneRIF: Tanc2-mediated mTOR inhibition balances mTORC1/2 signaling
            in the developing mouse brain and human neurons.
            Erratum:[Nat Commun. 2022 Nov 1;13(1):6544. doi:
            10.1038/s41467-022-33972-9.. PMID: 36319630]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12511)
  AUTHORS   Guo,H., Bettella,E., Marcogliese,P.C., Zhao,R., Andrews,J.C.,
            Nowakowski,T.J., Gillentine,M.A., Hoekzema,K., Wang,T., Wu,H.,
            Jangam,S., Liu,C., Ni,H., Willemsen,M.H., van Bon,B.W., Rinne,T.,
            Stevens,S.J.C., Kleefstra,T., Brunner,H.G., Yntema,H.G., Long,M.,
            Zhao,W., Hu,Z., Colson,C., Richard,N., Schwartz,C.E., Romano,C.,
            Castiglia,L., Bottitta,M., Dhar,S.U., Erwin,D.J., Emrick,L.,
            Keren,B., Afenjar,A., Zhu,B., Bai,B., Stankiewicz,P., Herman,K.,
            Mercimek-Andrews,S., Juusola,J., Wilfert,A.B., Abou Jamra,R.,
            Buttner,B., Mefford,H.C., Muir,A.M., Scheffer,I.E., Regan,B.M.,
            Malone,S., Gecz,J., Cobben,J., Weiss,M.M., Waisfisz,Q.,
            Bijlsma,E.K., Hoffer,M.J.V., Ruivenkamp,C.A.L., Sartori,S., Xia,F.,
            Rosenfeld,J.A., Bernier,R.A., Wangler,M.F., Yamamoto,S., Xia,K.,
            Stegmann,A.P.A., Bellen,H.J., Murgia,A. and Eichler,E.E.
  CONSRTM   University of Washington Center for Mendelian Genomics
  TITLE     Disruptive mutations in TANC2 define a neurodevelopmental syndrome
            associated with psychiatric disorders
  JOURNAL   Nat Commun 10 (1), 4679 (2019)
   PUBMED   31616000
  REMARK    GeneRIF: Pediatric patients with disruptive TANC2 mutations present
            with autism, intellectual disability, and delayed language and
            motor development. In addition to a variable degree of epilepsy and
            facial dysmorphism, we observe a pattern of more complex
            psychiatric dysfunction or behavioral problems in adult probands or
            carrier parents.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12511)
  AUTHORS   Stucchi,R., Plucinska,G., Hummel,J.J.A., Zahavi,E.E., Guerra San
            Juan,I., Klykov,O., Scheltema,R.A., Altelaar,A.F.M. and
            Hoogenraad,C.C.
  TITLE     Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca2+/CaM
            Controls DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to
            Postsynaptic Sites
  JOURNAL   Cell Rep 24 (3), 685-700 (2018)
   PUBMED   30021165
REFERENCE   5  (bases 1 to 12511)
  AUTHORS   Gasparini,A., Tosatto,S.C.E., Murgia,A. and Leonardi,E.
  TITLE     Dynamic scaffolds for neuronal signaling: in silico analysis of the
            TANC protein family
  JOURNAL   Sci Rep 7 (1), 6829 (2017)
   PUBMED   28754924
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12511)
  AUTHORS   Mahmood,S.F., Gruel,N., Chapeaublanc,E., Lescure,A., Jones,T.,
            Reyal,F., Vincent-Salomon,A., Raynal,V., Pierron,G., Perez,F.,
            Camonis,J., Del Nery,E., Delattre,O., Radvanyi,F. and
            Bernard-Pierrot,I.
  TITLE     A siRNA screen identifies RAD21, EIF3H, CHRAC1 and TANC2 as driver
            genes within the 8q23, 8q24.3 and 17q23 amplicons in breast cancer
            with effects on cell growth, survival and transformation
  JOURNAL   Carcinogenesis 35 (3), 670-682 (2014)
   PUBMED   24148822
  REMARK    GeneRIF: -RAD21 and EIF3H, both on chromosome 8q23, CHRAC1 on
            chromosome 8q24.3 and TANC2 on chromosome 17q23-were confirmed to
            be driver genes regulating the proliferation/survival of clonogenic
            breast cancer cells
REFERENCE   7  (bases 1 to 12511)
  AUTHORS   Han,S., Nam,J., Li,Y., Kim,S., Cho,S.H., Cho,Y.S., Choi,S.Y.,
            Choi,J., Han,K., Kim,Y., Na,M., Kim,H., Bae,Y.C., Choi,S.Y. and
            Kim,E.
  TITLE     Regulation of dendritic spines, spatial memory, and embryonic
            development by the TANC family of PSD-95-interacting proteins
  JOURNAL   J Neurosci 30 (45), 15102-15112 (2010)
   PUBMED   21068316
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC005972.1, AC006270.1, AC015923.8 and AC005828.1.
            
            On May 21, 2021 this sequence version replaced XM_006721806.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.823515.1,
                                           SRR14038193.3630514.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000689528.1/ ENSP00000510600.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-515               AC005972.1         134777-135291
            516-605             AC005972.1         178079-178168
            606-677             AC006270.1         139643-139714       c
            678-860             AC006270.1         114300-114482       c
            861-971             AC006270.1         62277-62387         c
            972-1120            AC006270.1         19518-19666         c
            1121-1307           AC006270.1         12700-12886         c
            1308-1571           AC015923.8         63076-63339         c
            1572-1697           AC015923.8         33280-33405         c
            1698-1979           AC005828.1         17350-17631
            1980-2113           AC005828.1         21919-22052
            2114-2345           AC005828.1         43063-43294
            2346-2512           AC005828.1         54212-54378
            2513-3120           AC005828.1         57745-58352
            3121-3229           AC005828.1         82680-82788
            3230-3352           AC005828.1         91597-91719
            3353-3589           AC005828.1         92270-92506
            3590-3775           AC005828.1         98705-98890
            3776-3869           AC005828.1         101783-101876
            3870-4003           AC005828.1         108084-108217
            4004-4127           AC005828.1         109116-109239
            4128-4303           AC005828.1         114473-114648
            4304-4436           AC005828.1         114960-115092
            4437-4466           AC005828.1         115642-115671
            4467-4558           AC005828.1         116505-116596
            4559-4705           AC005828.1         118490-118636
            4706-4806           AC005828.1         121269-121369
            4807-12511          AC005828.1         122961-130665
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.2-q23.3"
     gene            1..12511
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /note="tetratricopeptide repeat, ankyrin repeat and
                     coiled-coil containing 2"
                     /db_xref="GeneID:26115"
                     /db_xref="HGNC:HGNC:30212"
                     /db_xref="MIM:615047"
     exon            1..515
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            516..605
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     CDS             539..6763
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /note="isoform 2 is encoded by transcript variant 1;
                     putative ankyrin-repeat containing protein; rolling
                     pebbles homolog B; protein TANC2; tetratricopeptide
                     repeat, ankyrin repeat and coiled-coil domain-containing
                     protein 2"
                     /codon_start=1
                     /product="protein TANC2 isoform 2"
                     /protein_id="NP_001381927.1"
                     /db_xref="CCDS:CCDS92376.1"
                     /db_xref="GeneID:26115"
                     /db_xref="HGNC:HGNC:30212"
                     /db_xref="MIM:615047"
                     /translation="MFRNSLKMLLTGGKSSRKNRSSDGGSEEPPDRRQSSVDSRQSRS
                     GQGGISTESDCAFEPDYAVPPLPVSEGMQHIRIMEGMSRSLPSSPLLTHQSISVRLQP
                     VKKLTAPLRKAKFVESPRIPESELGSPTLTSAQKLDVDAYCPGDAEQELGPPPSVDEA
                     ANTLMTRLGFLLGEKVTEVQPGDQYSMEVQDENQTSAITQRISPCSTLTSSTASPPAS
                     SPCSTLPPISTNATAKDCSYGAVTSPTSTLESRDSGIIATLTSYSENVERTKYAGESS
                     KELGSGGNIKPWQSQKSSMDSCLYRVDENMTASTYSLNKIPERNLETVLSQSVQSIPL
                     YLMPRPNSVAATSSAHLEDLAYLDEQRHTPLRTSLRMPRQSMGGARTQQDLRVRFAPY
                     RPPDISLKPLLFEVPSITTESVFVGRDWVFHEIDAQLQSSNASVNQGVVIVGNIGFGK
                     TAIISRLVALSCHGTRMRQIASDSPHASPKHVDANRELPLTQPPSAHSSITSGSCPGT
                     PEMRRRQEEAMRRLASQVVAYHYCQADNAYTCLVPEFVHNVAALLCRSPQLTAYREQL
                     LREPHLQSMLSLRSCVQDPMASFRRGVLEPLENLHKERKIPDEDFIILIDGLNEAEFH
                     KPDYGDTIVSFLSKMIGKFPSWLKLIVTVRTSLQEITKLLPFHRIFLDRLEENEAIDQ
                     DLQAYILHRIHSSSEIQNNISLNGKMDNTTFGKLSSHLKTLSQGSYLYLKLTFDLIEK
                     GYLVLKSSSYKVVPVSLSEVYLLQCNMKFPTQSSFDRVMPLLNVAVASLHPLTDEHIF
                     QAINAGSIEGTLEWEDFQQRMENLSMFLIKRRDMTRMFVHPSFREWLIWREEGEKTKF
                     LCDPRSGHTLLAFWFSRQEGKLNRQQTIELGHHILKAHIFKGLSKKVGVSSSILQGLW
                     ISYSTEGLSMALASLRNLYTPNIKVSRLLILGGANINYRTEVLNNAPILCVQSHLGYT
                     EMVALLLEFGANVDASSESGLTPLGYAAAAGYLSIVVLLCKKRAKVDHLDKNGQCALV
                     HAALRGHLEVVKFLIQCDWTMAGQQQGVFKKSHAIQQALIAAASMGYTEIVSYLLDLP
                     EKDEEEVERAQINSFDSLWGETALTAAAGRGKLEVCRLLLEQGAAVAQPNRRGAVPLF
                     STVRQGHWQIVDLLLTHGADVNMADKQGRTPLMMAASEGHLGTVDFLLAQGASIALMD
                     KEGLTALSWACLKGHLSVVRSLVDNGAATDHADKNGRTPLDLAAFYGDAEVVQFLVDH
                     GAMIEHVDYSGMRPLDRAVGCRNTSVVVTLLKKGAKIGCQTLPSRPRGPATWAMATSK
                     PDIMIILLSKLMEEGDMFYKKGKVKEAAQRYQYALKKFPREGFGEDLKTFRELKVSLL
                     LNLSRCRRKMNDFGMAEEFATKALELKPKSYEAYYARARAKRSSRQFAAALEDLNEAI
                     KLCPNNREIQRLLLRVEEECRQMQQPQQPPPPPQPQQQLPEEAEPEPQHEDIYSVQDI
                     FEEEYLEQDVENVSIGLQTEARPSQGLPVIQSPPSSPPHRDSAYISSSPLGSHQVFDF
                     RSSSSVGSPTRQTYQSTSPALSPTHQNSHYRPSPPHTSPAHQGGSYRFSPPPVGGQGK
                     EYPSPPPSPLRRGPQYRASPPAESMSVYRSQSGSPVRYQQETSVSQLPGRPKSPLSKM
                     AQRPYQMPQLPVAVPQQGLRLQPAKAQIVRSNQPSPAVHSSTVIPTGAYGQVAHSMAS
                     KYQSSQGDIGVSQSRLVYQGSIGGIVGDGRPVQHVQASLSAGAICQHGGLTKEDLPQR
                     PSSAYRGGVRYSQTPQIGRSQSASYYPVCHSKLDLERSSSQLGSPDVSHLIRRPISVN
                     PNEIKPHPPTPRPLLHSQSVGLRFSPSSNSISSTSNLTPTFRPSSSIQQMEIPLKPAY
                     ERSCDELSPVSPTQGGYPSEPTRSRTTPFMGIIDKTARTQQYPHLHQQNRTWAVSSVD
                     TVLSPTSPGNLPQPESFSPPSSISNIAFYNKTNNAQNGHLLEDDYYSPHGMLANGSRG
                     DLLERVSQASSYPDVKVARTLPVAQAYQDNLYRQLSRDSRQGQTSPIKPKRPFVESNV
                     "
     exon            606..677
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            678..860
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            861..971
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1120
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1307
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1571
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1697
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1979
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2113
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2345
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2512
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2513..3120
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..3229
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3352
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3589
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            3590..3775
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3869
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            3870..4003
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4004..4127
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4128..4303
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4304..4436
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4466
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4467..4558
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4559..4705
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4806
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     exon            4807..12511
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12488..12493
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /note="hexamer: TATAAA"
     polyA_site      12511
                     /gene="TANC2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /note="major polyA site"
ORIGIN      
        1 ggcggcgcta gcgagcggcc ggcggggcgc gggttgctgg gcgcgctgct ccggcggggc
       61 ccgctggcac agccgcctcg cgcgagccgc ccgcccggcg ggggaagctg cgagcgctcc
      121 gagcgcccgg cctagggccg ctggcgctgc cctcccgccg ccaccgccct ccgcgcctcc
      181 ttcgggcggc cccgccccca ttcccatccc cctcgcgctc acctcccggc tgtgccgcgc
      241 gctaggcgga gcgaggcgcc cgccgccgcc gagccgagcc gaggggcgag agctggcccc
      301 cgagccgccg ctgacaggag caccgccgcg ggctgcgctc gccggctgcg aggccccgcc
      361 gcgccgttcc ggggtgcaga ctcgccgcgg ggcgttggag gactgcgagg tgctccggga
      421 atcgcgggcg gcgccggcgg gcggcgcggt cctcacagga gaccccgggg actggggctt
      481 gacccggagg tgcagagagc ccctcagagc ggcagttttg cagtagaaga gtataaccat
      541 gtttcggaat agtctcaaga tgctgcttac tggtgggaaa tcaagtcgta aaaacaggtc
      601 aagtgatgga gggagcgagg aaccaccgga tcgaagacag tcaagtgtag actctcgcca
      661 aagccgctct gggcaaggtg gcatctccac agaaagcgac tgtgcttttg agccagacta
      721 cgctgtcccg ccacttccag tgagtgaagg tatgcagcac attcggatta tggagggcat
      781 gtctcgctct cttccatcct cccccttact cacacatcag tctatcagtg ttcggctcca
      841 gcctgtgaag aagttaactg cccctctgag gaaggcaaag tttgttgaga gcccacgaat
      901 tccagaatcc gagcttggct caccaaccct cacttcagct cagaaactgg acgtggatgc
      961 atactgccct ggtgatgctg aacaggagct tggcccccct ccatctgtag atgaggcagc
     1021 aaacacactc atgactcgtc tgggtttcct ccttggagag aaagtcacag aagttcagcc
     1081 aggtgaccaa tacagcatgg aagtacagga tgaaaatcag acctcagcaa tcacccagcg
     1141 gataagtcct tgttccactc tgactagcag cactgcctct ccaccagcca gtagcccctg
     1201 ctctacactc ccacccatca gtacaaatgc aactgccaag gactgcagct atggggctgt
     1261 tactagtcca acctctaccc ttgaaagcag agatagtggc atcattgcta cattaacaag
     1321 ctattctgaa aatgtggaac gcacaaaata tgctggggaa agcagtaaag aattaggatc
     1381 tggaggaaac ataaaacctt ggcagtctca aaaatccagc atggactcct gtttgtatcg
     1441 agtagatgaa aacatgactg cttccaccta tagtctgaat aagatcccag agagaaattt
     1501 ggaaacagtg ttatctcaat cagtacagtc tattcctttg tatctcatgc cacggccaaa
     1561 ttcagtagca gccaccagct ctgcccactt ggaagacctt gcttacctgg atgagcagag
     1621 acatacacct ttgagaactt ctctacgaat gccaagacag agcatgggtg gtgctcgcac
     1681 acagcaggat ttaagagttc gctttgcacc ttataggcct ccagatatct ccttgaagcc
     1741 tctgctcttt gaagtgccca gcatcactac agagtcagtg tttgttggcc gagattgggt
     1801 tttccacgaa atagatgctc aacttcaaag ttcaaatgcc agcgtgaacc aaggagtagt
     1861 gattgtggga aacattggat tcggcaaaac tgccatcatc tccagactgg tggccctcag
     1921 ctgccatggt acaaggatga gacagatcgc ctcagacagc ccacatgcct cccccaaaca
     1981 tgtggatgcc aacagagagc tgccactcac acagccacct tcagcccact catctatcac
     2041 cagtggaagc tgcccaggaa ctccggaaat gcgcaggcgg caggaggagg ctatgcgaag
     2101 actagcctcg caggtggttg cctatcacta ttgtcaagca gataatgcct acacttgctt
     2161 ggtgccagaa tttgtccaca atgttgctgc cttgctctgc cgctcacctc agctgacagc
     2221 ctatcgggag cagcttcttc gggaacctca cctgcagagc atgctgagcc ttcgttcctg
     2281 tgttcaagac cccatggcct ccttccggag gggagttctg gagccactag aaaatctcca
     2341 taaagagaga aaaatcccag atgaagattt catcatttta attgatggat taaatgaagc
     2401 agaatttcac aaaccggatt atggggatac aattgtatcg tttctgagta aaatgatcgg
     2461 aaagtttcct tcttggctca aactaattgt aacagttagg accagtttac aggaaattac
     2521 caagctgctg cctttccata ggattttttt ggatcgacta gaagagaatg aagccataga
     2581 ccaggacctg caggcttaca tcctgcaccg gatacacagc agctcagaga tccagaataa
     2641 catttcactt aatggcaaaa tggacaatac tacatttggc aaactcagtt ctcatctcaa
     2701 gaccctcagt caagggtcct atctatatct gaaacttaca tttgacctca tagagaaagg
     2761 ctatctagtg ttaaagagct ctagctacaa agtagttcct gtttcgctct cagaggttta
     2821 tttactccag tgcaatatga agttcccaac ccagtcttcc tttgaccggg tgatgcctct
     2881 cctgaatgtg gcagtggcct ctctccaccc actgactgat gagcatatct tccaggccat
     2941 caatgctggg agcattgaag gcacactaga atgggaggat tttcagcaga gaatggagaa
     3001 cctctccatg ttcctaatca agcgcagaga catgactcgt atgtttgtac atccttcttt
     3061 tcgagaatgg cttatctgga gagaagaagg agagaaaacc aaatttctct gtgatccgag
     3121 gagtggtcac acgttacttg ccttctggtt ttcccgccaa gagggaaaac taaaccgaca
     3181 gcagactatt gaactgggac atcacatcct caaagcacac atttttaagg gtttgagtaa
     3241 aaaagttggt gtatcatcct ccatcctcca aggtctctgg atctcttata gcacagaagg
     3301 tctttccatg gcactggcgt ctttacgaaa tctctacact ccaaatataa aggtcagccg
     3361 actgctgatt ttgggaggtg ccaatattaa ttaccggaca gaggttttaa ataatgctcc
     3421 aattctatgt gttcagtccc atcttggtta cacagaaatg gtagccctgc tgctggagtt
     3481 cggggccaac gtggatgcct cttctgaaag tggcctgact cccctgggat atgctgcagc
     3541 agcagggtac ctgagcattg tggtgctgct gtgcaagaaa cgggccaagg tggatcattt
     3601 ggataagaac gggcagtgtg ctttggttca tgctgcactc cgaggtcatc tggaggttgt
     3661 caagtttttg attcagtgtg actggacgat ggccggccag cagcaaggag tatttaagaa
     3721 gagccatgcc atccaacagg ccctcattgc tgcagccagc atgggttata ctgagattgt
     3781 ctcctaccta cttgatcttc cagaaaaaga tgaagaggaa gtagagcgag cacagatcaa
     3841 cagctttgac agtctctggg gagagacagc cctaacagct gcagccggaa ggggcaaact
     3901 ggaggtgtgc cgtttgctct tggaacaagg ggcggcagtg gcccagccaa accgccgagg
     3961 agcagtgcca ctattcagca cagtgcgcca gggccactgg cagattgttg atcttttact
     4021 cacccatgga gctgatgtca acatggcaga caagcagggc cgcactcccc tgatgatggc
     4081 tgcttccgaa ggccatctag gaaccgtgga ctttctgctt gcacaaggtg cctccattgc
     4141 tcttatggac aaagaaggat tgacagccct cagctgggct tgtttgaagg gccatctctc
     4201 agtagtacgt tctctggtgg ataacggagc tgccacagac catgctgaca agaatggccg
     4261 taccccactg gatctggcag ctttctatgg cgatgctgag gtggtccagt tcctggtaga
     4321 tcatggggcc atgatcgagc acgttgacta cagtggaatg cgccctttgg atagggcagt
     4381 ggggtgccgg aacacttctg ttgttgtcac tcttctgaag aaaggagcca agataggttg
     4441 tcagacgtta ccgagtcgcc cacgaggtcc agccacatgg gcgatggcca cctccaagcc
     4501 agacatcatg atcatcctgt tgagcaagct gatggaagag ggggacatgt tttataagaa
     4561 aggtaaagta aaggaagctg cccagcgcta ccagtacgcc ctgaagaagt tccctagaga
     4621 agggtttggt gaggacttga aaactttccg ggaactaaag gtgtctctcc tcctcaacct
     4681 ctctcggtgt cgcaggaaaa tgaacgattt tggaatggcg gaggaatttg ctactaaggc
     4741 cctggagctg aaaccgaaat cttatgaagc ttactatgcg agagcaaggg caaaacgcag
     4801 cagcagacag ttcgcagcag ccttagagga cctgaacgag gccatcaagc tgtgtcccaa
     4861 caaccgtgag atccagagac ttctgctgag agtggaagaa gagtgtagac agatgcagca
     4921 gccacagcag ccaccgccgc caccgcagcc tcagcagcag ttgccggaag aagcagaacc
     4981 tgagccacag catgaagaca tatactctgt acaggatata ttcgaggagg agtacctgga
     5041 acaggatgtt gaaaatgttt ccattggcct ccagacagag gcccggccca gccaggggct
     5101 cccggtcatc cagagcccac cctcctctcc cccgcatcgg gactcagcct acatctccag
     5161 ctcacctctt ggctctcatc aggtttttga cttccggtcc agtagttctg taggctctcc
     5221 cactagacag acctatcagt ccacctcacc tgccctttct ccaactcatc agaactcaca
     5281 ttacaggcct agcccaccac acacttcccc ggctcatcag ggaggatctt accgtttcag
     5341 cccccctcct gtgggaggac agggcaaaga atacccaagc cctccccctt cccctctccg
     5401 gagaggccct cagtatcggg ccagccctcc agctgaaagt atgagtgtct atagatccca
     5461 gtctggttca cccgtgcgct atcagcagga aacaagcgtc agtcagcttc ctggcagacc
     5521 caaatctcca ttatccaaaa tggcccagcg gccctaccag atgcctcagc tccctgtggc
     5581 agttccccag caagggctca ggctacagcc tgccaaggcc cagattgtga gaagtaacca
     5641 gcccagccca gccgtccatt caagcaccgt catccccaca ggagcctatg gccaagtagc
     5701 ccattcaatg gccagtaaat accagtcttc acaaggagac ataggagtca gccagagccg
     5761 gttggtttat caagggtcaa ttgggggaat cgtaggggat ggaaggccgg tgcagcatgt
     5821 ccaagccagc ctgagtgcag gcgccatctg tcagcatgga ggattgacca aagaggatct
     5881 tccacagcga ccttcctcag cataccgagg tggcgtgaga tacagccaga caccacagat
     5941 cggacgcagc cagtcagcat cctattaccc agtctgtcac tcaaaactag atctggagcg
     6001 ctcctccagc caactaggtt cccctgatgt gtcgcattta atcagaagac ctatcagtgt
     6061 caaccctaac gaaatcaaac cgcacccgcc aactcccagg ccgttgctgc attcccaaag
     6121 tgtaggcctt cgcttctctc catctagcaa tagtatctcc tccacctcca acctaactcc
     6181 gaccttccgg ccatcttctt ccatccagca aatggagatc ccactgaaac ctgcatatga
     6241 gaggtcatgt gacgagctgt cgccagtgtc tccaactcaa ggaggttacc ccagtgagcc
     6301 cacccgatcc aggaccacac cattcatggg gatcatagat aaaacagcac ggactcagca
     6361 gtacccccac ctccaccagc agaatcggac ctgggcagtg tcatctgtgg acaccgtcct
     6421 cagtcccacg tctccaggca acctgcctca gcctgagtcc ttcagtccac catcatccat
     6481 cagcaacatt gccttttata acaaaaccaa caatgcacag aatggccatt tgctggagga
     6541 cgattattac agcccccatg ggatgctggc taacgggtct cgtggagacc tcttggagcg
     6601 agtcagccag gcctcctcct atcccgacgt gaaggtagct cggactctac ctgtggctca
     6661 ggcataccag gacaacctgt acaggcagct gtcccgagac tctcggcaag ggcagacatc
     6721 ccctatcaaa ccaaagagac cgttcgtgga gtctaatgtt taaaagacgt tttgttggag
     6781 tgagacccat atgttttcac tgcacatttt caggcttggt ttccacattc gaggtagttc
     6841 tctggcttaa tttctcatgt agtttctgtg tggtgttcag aggtggcagc ccacatgctg
     6901 aaatcctttg catgcagccg actgggaagc ggcctcccgg gagccaggac ttcagtttct
     6961 cttgtctgtg cccagccaca tgctctctcc ctctcttcag atgccaacga ggagattttc
     7021 gtgctgtgtg ctttaaccca gggagatcag acacactggt cagctttttc caggagacaa
     7081 tcgctttcac tgatgttctt gttgtgtaat tgtctttttc cttttttaaa aaataaggtg
     7141 ttcttgttcg ttttcttcta gaaactttag aaagagtgcg atgccccttt gcctttgcat
     7201 ccttagccag tgtcacccac acagccagcc gcagcgcatt ctcatgctgt ggcccctccc
     7261 cagaccgcca gcgccctgca gccaccaggt ctgcagtgtg cattaggatt attgctggtc
     7321 ttcctagggg gtaaaaggat cagagagaga agaattaagt gctaaattgg aagaaaaccc
     7381 caatataatt atgtaaaatg tcactacatt gattttccaa gaggcattgt aggaacatgt
     7441 caaaaacagc cagcccttta aatattgcag tcagccaagg aaattagatg agaattgtgg
     7501 ctattaagag aattcactga gagttattct ctagattttt agccgacaat taaccactaa
     7561 aagctgctgc ttttccaggg tgggggaggg aatgaataca tagaaaaaca aaaaagattg
     7621 ttctggattc tcagtgaaag gctataggaa gtctgttctg gagacatcta ctttttagat
     7681 cctgatacat cactgagtgt catactccac taaaaggaaa ctctaaccga aggctggctg
     7741 gtgtgacaat cccgttagtt ggatcttcac ctacagccag attttgttct agtggccctc
     7801 ttcctgtaaa cccagatggt gtcatacaga aattgtttct ttcagaagca gattggaatc
     7861 tcttgggacc atgagactga gtcccaatat ttccacccag ggtcatgccc gttgttgtct
     7921 acttccattt tgagatctat agtttgatta tctattatta caggaactgt ttcttttctt
     7981 tttctaggag tgtttatgag agtgtgatat tttaaagtca gacgcagcaa aaactgtttc
     8041 agggtgaaga aagacccctt tcagccctgt tttgcagccc tgggtggggg catgagatag
     8101 acagcaagct tctgatcttg aagcttgtct agaagacaca tcttctaggt ctcgtggtca
     8161 tttggtaggc tgacctttga gtgagcgagg ccactattga gtggatagca agaacattgg
     8221 aaccaaagcc tcggcacagg cctggcactg gctgtacatc agctcttaca actaaacaac
     8281 tcaactaagc aactgaaacg aaacaaagga gcattcgttc tctgttgtta ggaatcattc
     8341 tgctctgtta gggaagggct gcaggaaggg cagtttcctg aataaaaatc tggctgcgac
     8401 cagtcccatg tgtctggtaa gtaagtaagt aagtaagtgc cctttgaagg gatcattaag
     8461 acacagggag catgaacctg agatcagaag catttcttta ctaatttaga ttctgcgaaa
     8521 tagacggacc tctccacccc caaacctaaa acaggccagg acttgtctct gtgctgaaag
     8581 caaatagcaa gactaactca agccccagcc tctttccaca ctccctgata cctaaggact
     8641 gctttctcag ctagaccagg gtgggcatca gcgacgcctt ctcagctaga ccagggtagg
     8701 catcagcgtc ctctctccat ctctataccc ctctctctca catcaggaag atgaaatgtg
     8761 tagctctagc ggcaacctct agccaggagc ccagtggcct ctcagattgc tttttggcca
     8821 ggtctcagca ctgctggcat ctttacatct tactccttaa aaccgcctct cgctgaggag
     8881 ccactgcatt ttgaagaatt tctcagtgtc tgtcaggaaa gtgctcctgc tcatttggaa
     8941 cgccacacac cacccgcact cacctgtcca ggcgaatgag caggtcctgt agctttacaa
     9001 atatgccgct gatgccgctt ctccccaggg tctctcagtt ttccaggaca aaaagattta
     9061 gggcctatac cctatgggca aaacacctaa aatgtcaaca gtcaaaatgc cattcttttt
     9121 ggccatcata agagggagta ggtatcactg ctgcatgcca gttgtttttg actagaatat
     9181 gccaaccaga gcttgttggg gcaggagacg tttttcctta caagcagact gcctgtgccc
     9241 tgtgccctgt ttgctacttc actgccatgg aatgatccga gtactgtatt tcagagctgc
     9301 cccttcccca gcagcaaaca ctcgctgagt ccatgtctgg cttcaggtgg gaggaaatgt
     9361 ttcagatgaa acttactcaa ttcataccac cctgaaatgg aggacagagg tgacaaactt
     9421 cagtttaata ggtttctcac caagttgtat gttccattgg cccaggattc ttgcactaat
     9481 gggtttctat cacatcatgt ctataaatgg gtgcacttta ctgtttgaat ttgtaactga
     9541 agtactggat atttaagtgt gagtaatgtc ttcattagaa aatagcagaa ccgctcttgt
     9601 cttttagtgt atttttcaag aaaaaaggaa aggaaagaca tcaagcagtg gatcacaaca
     9661 tttatagcac aagaaataac ttgtatataa gcatcaaaaa gattaagaat tttttaatat
     9721 gaaaaatatt tgcagtgatt ttaaagtgct tttccagcaa tgttcttagg gactcctgag
     9781 acacggttac tttatctact ggatcagtaa ggcacacaat taacaattaa caattaatgt
     9841 ttatttacaa agtaaaggga aaacctgtgt aacatgagaa tttggcatga caaaatggag
     9901 accattttgt atctgctgtt gtatcttgtc cgggttgcag acgtgcacta ttaaagtccc
     9961 aagttaatag agcacaaacc cttctcgctc ctcccccatg tgcccctctt tttagatgtg
    10021 tataacttaa actcgatggc tcaggaaaat tccactaatt agaatcatgt acagtacccc
    10081 aggtcgttgt ccagatatac aagtttgcta atgtagttaa gcctggatta ttaaacactt
    10141 ttcctaaatt attgtaaaca gaacagctta gagaaaggta ttctcagtcc ttaatattgt
    10201 atagtagttt atgagcccct ctctaaatat tggtattttt atattccaga gatgtaccca
    10261 atagaaaaaa ttaaaaatta atcagtatct aatttaatat ccataagtat ttttccttag
    10321 attttagtca cgtacagtgg gctatgtgga tgtcacttgt gcttcaccat agtttaccac
    10381 taggtgtcac tgtggctctg cactgcgctt gttttgtagc aaagaacagc ggcatcccct
    10441 cgggagagag gagctgcttc cagggcaaca ggcaagcggg ctcagaggtt caggagaagg
    10501 caacagaggc ctggaagggg tcttcgtgca tctgtgccag ttgtgcaaga cgaactcttt
    10561 gaacactaca tgctttggac ttcagccagg cagaggctgg aagaaggttg accagagctc
    10621 ccttgctctg gtagagggat gggtacatgg agaagcccct tcttccccat gagcctccct
    10681 cctgtcagtt cctctcagcc tccagctttt ataactccag aagcgtcaca gttgggtggt
    10741 ttgattcaga gagagttatt tttctactgc agaaatgcct tggacaaaac cagtgctcac
    10801 tgaatctttg ccacaaaatg gaataggcta tcccaggggg caagaggtgc ccgcccctgt
    10861 gcccagcctc ctcttgatgc tcccagtgcc cagcagcctc gcacaccctg cctgtctgtt
    10921 cctgggctgc ccatttctca agaaaccgac ctgcaaaggc agccggctgc tgcctccaca
    10981 ccgagggctg tgcggtcctg ctgctcgctc actgggaggt gcagctcttt ctcctcttcc
    11041 tctaggaatt ccagaccgac catctaccat gactaacaac aatgaacaaa gggcttaggg
    11101 gcaagagcta cctgcaaaga cgtgtcatgg aacccttcac catgcaatgc cttgaactca
    11161 gctctggctg ctcccaagaa aaggtggctg gctgggggcc tggacacaag cacaatgggg
    11221 ctggtggagc cactgtgcag agctacttga ataatcactg ggttttcatc aactcctttt
    11281 gtcatacaga ccactcaagg gctgaagtgt tggtaacctt catttcggtg tccaaagcct
    11341 cacagcaggt gagccaccct gagatgcttg tggccacatg gtggccacag tcagagcttt
    11401 gaaagtcagt accaaatgaa cgcataattg gacaccaaaa atcaagtgtt actttcatgt
    11461 ttcctcaccc catcatctca ttgcctcctg ctgactctga taccgacgct gagctgactt
    11521 gccaggctgc cgctggacgc gtagagatca ggccagcgcc gcgctcattt ttccaggtag
    11581 acctactctg tggaacggaa gtgccctagc tgctttgttt ttgtagcact tgctggctga
    11641 atttttcttt tgctaatcgc taaccagaaa gtctggttag agggggctca actcaatccc
    11701 tttggtcccc agcgccagac aagagttaat tctggaaaat tcagtacttg aatgtacctg
    11761 ccttattgca taccaattta ctggggggaa aaaaaaagtt aagagatgcc ggctccagat
    11821 ctccacttca ttcacaggtg attttggaaa tcctgtaagt tacacttcct gttctggttt
    11881 tgttttgttt tttgtttcct ttggctgatt cctgctgagt gaggccagtt cctcatcagg
    11941 ctcagggcag gtgccttttc aggcgtggcc tcctttccat ctagcacagc atctttgtct
    12001 ctgttctgtc tcctccaaat ccaagatgat tttaattagt acagacatgt acagtctaca
    12061 attaaagagt gatttgtact aatatgattt tgattcttcc tcctctttgc tgtcctttca
    12121 agacacttgc tggaaaaagc tttaatgcac ttagttttcc tttaggtttt ctatgactca
    12181 gatgtaaagg actttctctg tacagtatat tatccaatgc atgtttgttc tctctcctga
    12241 tatattgaac accacacagt tgtgaagccg tgcagtgggg atgccccaca ccccacagag
    12301 gcatctaccc ctgtgtataa ggaaagacat tttcctttgc tgtacttgct tgagcagttt
    12361 tattgtctgt acatgtgagc tgtgtgagat agatgtgaaa agttcaaatg aatgcatttt
    12421 cctgccccat gtatacagat tgtcatctgt acaaggaact gtatgtatga aagcaaatgt
    12481 acttatttat aaatggctaa cacttggaaa a
//
