LOCUS       NM_181711               1952 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens trafficking regulator and scaffold protein tamalin
            (TAMALIN), transcript variant 1, mRNA.
ACCESSION   NM_181711
VERSION     NM_181711.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Pandey,S., Ramsakha,N., Sharma,R., Gulia,R., Ojha,P., Lu,W. and
            Bhattacharyya,S.
  TITLE     The post-synaptic scaffolding protein tamalin regulates
            ligand-mediated trafficking of metabotropic glutamate receptors
  JOURNAL   J Biol Chem 295 (25), 8575-8588 (2020)
   PUBMED   32376687
  REMARK    GeneRIF: The post-synaptic scaffolding protein tamalin regulates
            ligand-mediated trafficking of metabotropic glutamate receptors.
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Matosin,N., Fernandez-Enright,F., Lum,J.S., Andrews,J.L., Engel,M.,
            Huang,X.F. and Newell,K.A.
  TITLE     Metabotropic glutamate receptor 5, and its trafficking molecules
            Norbin and Tamalin, are increased in the CA1 hippocampal region of
            subjects with schizophrenia
  JOURNAL   Schizophr Res 166 (1-3), 212-218 (2015)
   PUBMED   26048293
  REMARK    GeneRIF: Schizophrenia subjects compared to controls showed a
            marked increase in CA1 hippocampal Norbin, and Tamalin proteins
            (47% and 34% respectively), which are endogenous regulators of
            mGluR5 signalling and trafficking
REFERENCE   3  (bases 1 to 1952)
  AUTHORS   Fransen,E., Bonneux,S., Corneveaux,J.J., Schrauwen,I., Di
            Berardino,F., White,C.H., Ohmen,J.D., Van de Heyning,P.,
            Ambrosetti,U., Huentelman,M.J., Van Camp,G. and Friedman,R.A.
  TITLE     Genome-wide association analysis demonstrates the highly polygenic
            character of age-related hearing impairment
  JOURNAL   Eur J Hum Genet 23 (1), 110-115 (2015)
   PUBMED   24939585
REFERENCE   4  (bases 1 to 1952)
  AUTHORS   Heinrich,F., Nanda,H., Goh,H.Z., Bachert,C., Losche,M. and
            Linstedt,A.D.
  TITLE     Myristoylation restricts orientation of the GRASP domain on
            membranes and promotes membrane tethering
  JOURNAL   J Biol Chem 289 (14), 9683-9691 (2014)
   PUBMED   24505136
  REMARK    GeneRIF: myristoylated GRASP promoted tethering and exhibited a
            unique membrane complex. Thus, myristoylation restricts the
            membrane orientation of the GRASP domain favoring interactions in
            trans for membrane tethering.
REFERENCE   5  (bases 1 to 1952)
  AUTHORS   Attar,M.A. and Santy,L.C.
  TITLE     The scaffolding protein GRASP/Tamalin directly binds to Dock180 as
            well as to cytohesins facilitating GTPase crosstalk in epithelial
            cell migration
  JOURNAL   BMC Cell Biol 14, 9 (2013)
   PUBMED   23441967
  REMARK    GeneRIF: GRASP bridges the guanine nucleotide exchange factors
            (GEFs) that activate Arf and Rac, thereby promoting Arf-to-Rac
            signaling By binding to both cytohesin 2/ARNO and Dock180.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1952)
  AUTHORS   Sugi,T., Oyama,T., Muto,T., Nakanishi,S., Morikawa,K. and
            Jingami,H.
  TITLE     Crystal structures of autoinhibitory PDZ domain of Tamalin:
            implications for metabotropic glutamate receptor trafficking
            regulation
  JOURNAL   EMBO J 26 (8), 2192-2205 (2007)
   PUBMED   17396155
  REMARK    GeneRIF: The crystal structures of the autoinhibitory PDZ domain of
            Tamalin has implications for the regulation of metabotropic
            glutamate receptor trafficking.
REFERENCE   7  (bases 1 to 1952)
  AUTHORS   Kitano,J., Yamazaki,Y., Kimura,K., Masukado,T., Nakajima,Y. and
            Nakanishi,S.
  TITLE     Tamalin is a scaffold protein that interacts with multiple neuronal
            proteins in distinct modes of protein-protein association
  JOURNAL   J Biol Chem 278 (17), 14762-14768 (2003)
   PUBMED   12586822
REFERENCE   8  (bases 1 to 1952)
  AUTHORS   Kitano,J., Kimura,K., Yamazaki,Y., Soda,T., Shigemoto,R.,
            Nakajima,Y. and Nakanishi,S.
  TITLE     Tamalin, a PDZ domain-containing protein, links a protein complex
            formation of group 1 metabotropic glutamate receptors and the
            guanine nucleotide exchange factor cytohesins
  JOURNAL   J Neurosci 22 (4), 1280-1289 (2002)
   PUBMED   11850456
REFERENCE   9  (bases 1 to 1952)
  AUTHORS   Nevrivy,D.J., Peterson,V.J., Avram,D., Ishmael,J.E., Hansen,S.G.,
            Dowell,P., Hruby,D.E., Dawson,M.I. and Leid,M.
  TITLE     Interaction of GRASP, a protein encoded by a novel retinoic
            acid-induced gene, with members of the cytohesin family of guanine
            nucleotide exchange factors
  JOURNAL   J Biol Chem 275 (22), 16827-16836 (2000)
   PUBMED   10828067
REFERENCE   10 (bases 1 to 1952)
  AUTHORS   Dominguez,O., Ashhab,Y., Sabater,L., Belloso,E., Caro,P. and
            Pujol-Borrell,R.
  TITLE     Cloning of ARE-containing genes by AU-motif-directed display
  JOURNAL   Genomics 54 (2), 278-286 (1998)
   PUBMED   9828130
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY052304.1, BC053666.1 and
            BC052967.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_181711.3.
            
            Summary: This gene encodes a protein that functions as a molecular
            scaffold, linking receptors, including group 1 metabotropic
            glutamate receptors, to neuronal proteins. The encoded protein
            contains conserved domains, including a leucine zipper sequence,
            PDZ domain and a C-terminal PDZ-binding motif. Alternately spliced
            transcript variants have been observed for this gene.[provided by
            RefSeq, Dec 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279868.3230.1, BC053666.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000293662.9/ ENSP00000293662.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                HY052304.1         3-20
            19-1950             BC053666.1         1-1932
            1951-1952           BC052967.1         1636-1637
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..1952
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /note="trafficking regulator and scaffold protein tamalin"
                     /db_xref="GeneID:160622"
                     /db_xref="HGNC:HGNC:18707"
                     /db_xref="MIM:612027"
     exon            1..320
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1262
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     general receptor for phosphoinositides 1 associated
                     scaffold protein; GRP1 (general receptor for
                     phosphoinositides 1)-associated scaffold protein; protein
                     TAMALIN"
                     /codon_start=1
                     /product="protein TAMALIN isoform 1"
                     /protein_id="NP_859062.1"
                     /db_xref="CCDS:CCDS8817.1"
                     /db_xref="GeneID:160622"
                     /db_xref="HGNC:HGNC:18707"
                     /db_xref="MIM:612027"
                     /translation="MTLRRLRKLQQKEEAAATPDPAARTPDSEVAPAAPVPTPGPPAA
                     AATPGPPADELYAALEDYHPAELYRALAVSGGTLPRRKGSGFRWKNLSQSPEQQRKVL
                     TLEKEDNQTFGFEIQTYGLHHREEQRVEMVTFVCRVHESSPAQLAGLTPGDTIASVNG
                     LNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSL
                     MVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRA
                     RGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASV
                     RCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEE
                     SQL"
     exon            321..370
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     exon            371..425
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     exon            426..528
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     exon            529..622
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     exon            623..689
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     exon            690..756
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     exon            757..1952
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1931..1936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /note="hexamer: TATAAA"
     polyA_site      1952
                     /gene="TAMALIN"
                     /gene_synonym="GRASP"
                     /note="major polyA site"
ORIGIN      
        1 ggcatccccc agccgccgcc agccccgccg aggggagcca gcgccgtctc tgaggggcgt
       61 ccggcgccgg agccatgacc ctccgccgac tcaggaagct gcagcagaag gaggaggcgg
      121 cggccacccc ggaccccgcc gcccggactc ccgactcgga agtcgcgccc gccgctccgg
      181 tcccgacccc gggaccccct gccgcagccg ccacccctgg gcccccagcg gacgagctgt
      241 acgcggcgct ggaggactat caccctgccg agctgtaccg cgcgctcgcc gtgtccgggg
      301 gcaccctgcc ccgccgaaag ggctcaggat tccgctggaa gaatctcagc cagagtcctg
      361 aacagcagcg gaaagtgctg acgttggaga aggaggataa ccagaccttc ggctttgaga
      421 tccagactta tggccttcac caccgggagg agcagcgtgt ggaaatggtg acctttgtct
      481 gccgagttca tgagtctagc cctgcccagc tggctgggct cacaccaggg gacaccatcg
      541 ccagcgtcaa tggcctgaat gtggaaggca tccggcatcg agagattgtg gacatcatta
      601 aggcgtcagg caatgttctc agactggaaa ctctatatgg gacatcaatt cggaaggcag
      661 aactggaggc tcgtctgcag tacctgaagc aaaccctgta tgagaagtgg ggagagtaca
      721 ggtccctaat ggtgcaggag cagcggctgg tgcatggcct ggtggtgaag gaccccagca
      781 tctacgacac gctggagtcg gtgcgctcct gcctctacgg cgcgggcctg ctcccgggct
      841 cgctgccctt cgggcctctg ctcgccgtgc ccgggcgtcc ccgcggaggc gcccgacggg
      901 ccaggggcga cgccgacgac gccgtctacc acacgtgctt cttcggggac tccgagccgc
      961 cggcgctgcc gcccccgccg cccccggccc gcgccttcgg cccgggcccc gccgagaccc
     1021 ctgccgtggg gccgggccct gggccgcggg ccgcgctgag ccgcagcgcc agtgtgcggt
     1081 gcgcgggccc tggcgggggc ggaggcgggg gcgcgccggg cgcgctctgg actgaggctc
     1141 gcgagcaggc cctatgcggc cccggcctgc gcaaaaccaa gtaccgcagc ttccgccggc
     1201 ggctgctcaa gttcatcccc ggactcaacc gctccctgga ggaggaggag agccagctgt
     1261 aggggcgggg gcgggcaggg aggtatttat ttatttattc gcaacagcca gcgctaaaag
     1321 agggggaggc cgagccaaga ggaccccagg agcccagagc agcgggagag ggtccttcct
     1381 agcctcggcc cgccgggtcg gttcctggct ggtgtctgct gagggagtgg ggggcccagc
     1441 cccttctctt ctcccccgcc aaaccacagt gggagctggg gcagggggag agccaggcaa
     1501 tcgggggcca aagatggggg tgctcgccta cagtctgcat ctgtagtgcc ttgtggggta
     1561 tccaggaaca ccctcccagc aggggatggg aaccctgtcc catgaagccc tctcctcagc
     1621 tttacttgct cccccgccct tagccttggg gagaaatggc ccgtggtggg ctgacccccc
     1681 accctccaca cacacagttc catgacccag cgggccccca ggggcatcag gtgctggtcc
     1741 tcctccctcc tggcctcgac ccctaagggc ttcgcccctc ccaggggcct gtaactaagt
     1801 cgggtcctgc caggcagggg gcctgtgttc tgtgcccctt gggagacagg aactggcgag
     1861 ttcaggtggg gtggggacag cacagactgt tccaccgttg tgcatattgt tgcttctgaa
     1921 ccacaaactg tataaatgga tggttttttg ca
//
