LOCUS       NM_003186               3786 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens transgelin (TAGLN), transcript variant 2, mRNA.
ACCESSION   NM_003186
VERSION     NM_003186.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Barnes,E.A., Ito,R., Che,X., Alvira,C.M. and Cornfield,D.N.
  TITLE     Loss of prolyl hydroxylase 1 and 2 in SM22alpha-expressing cells
            prevents Hypoxia-Induced pulmonary hypertension
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 325 (6), L741-L755 (2023)
   PUBMED   37847687
  REMARK    GeneRIF: Loss of prolyl hydroxylase 1 and 2 in SM22alpha-expressing
            cells prevents Hypoxia-Induced pulmonary hypertension.
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Gu,X., Chen,S., Wang,Z., Bu,Q. and An,S.
  TITLE     LZTS3/TAGLN Suppresses Cancer Progression in Human Colorectal
            Adenocarcinoma Through Regulating Cell Proliferation, Migration,
            and Actin Cytoskeleton
  JOURNAL   Arch Med Res 54 (7), 102894 (2023)
   PUBMED   37806182
  REMARK    GeneRIF: LZTS3/TAGLN Suppresses Cancer Progression in Human
            Colorectal Adenocarcinoma Through Regulating Cell Proliferation,
            Migration, and Actin Cytoskeleton.
REFERENCE   3  (bases 1 to 3786)
  AUTHORS   Barnes,E.A., Knutsen,C., Kindt,A., Che,X., Ying,L., Adams,E.,
            Gonzalez,E., Oak,P., Hilgendorff,A., Alvira,C.M. and Cornfield,D.N.
  TITLE     Hypoxia-Inducible Factor-1alpha in SM22alpha-Expressing Cells
            Modulates Alveolarization
  JOURNAL   Am J Respir Cell Mol Biol 69 (4), 470-483 (2023)
   PUBMED   37290124
  REMARK    GeneRIF: Hypoxia-Inducible Factor-1alpha in SM22alpha-Expressing
            Cells Modulates Alveolarization.
REFERENCE   4  (bases 1 to 3786)
  AUTHORS   Dong,L.H., Lv,P. and Han,M.
  TITLE     Roles of SM22alpha in cellular plasticity and vascular diseases
  JOURNAL   Cardiovasc Hematol Disord Drug Targets 12 (2), 119-125 (2012)
   PUBMED   23030444
  REMARK    Review article
REFERENCE   5  (bases 1 to 3786)
  AUTHORS   Assinder,S.J., Stanton,J.A. and Prasad,P.D.
  TITLE     Transgelin: an actin-binding protein and tumour suppressor
  JOURNAL   Int J Biochem Cell Biol 41 (3), 482-486 (2009)
   PUBMED   18378184
  REMARK    GeneRIF: acts as a tumour suppressor; expression is lost in
            prostate, breast and colon cancers
            Review article
REFERENCE   6  (bases 1 to 3786)
  AUTHORS   Camoretti-Mercado,B., Forsythe,S.M., LeBeau,M.M., Espinosa,R. 3rd,
            Vieira,J.E., Halayko,A.J., Willadsen,S., Kurtz,B., Ober,C.,
            Evans,G.A., Thweatt,R., Shapiro,S., Niu,Q., Qin,Y., Padrid,P.A. and
            Solway,J.
  TITLE     Expression and cytogenetic localization of the human SM22 gene
            (TAGLN)
  JOURNAL   Genomics 49 (3), 452-457 (1998)
   PUBMED   9615232
REFERENCE   7  (bases 1 to 3786)
  AUTHORS   Yamamura,H., Masuda,H., Ikeda,W., Tokuyama,T., Takagi,M.,
            Shibata,N., Tatsuta,M. and Takahashi,K.
  TITLE     Structure and expression of the human SM22alpha gene, assignment of
            the gene to chromosome 11, and repression of the promoter activity
            by cytosine DNA methylation
  JOURNAL   J Biochem 122 (1), 157-167 (1997)
   PUBMED   9276683
REFERENCE   8  (bases 1 to 3786)
  AUTHORS   Kobayashi,R., Kubota,T. and Hidaka,H.
  TITLE     Purification, characterization, and partial sequence analysis of a
            new 25-kDa actin-binding protein from bovine aorta: a SM22 homolog
  JOURNAL   Biochem Biophys Res Commun 198 (3), 1275-1280 (1994)
   PUBMED   8117285
REFERENCE   9  (bases 1 to 3786)
  AUTHORS   Thweatt,R., Lumpkin,C.K. Jr. and Goldstein,S.
  TITLE     A novel gene encoding a smooth muscle protein is overexpressed in
            senescent human fibroblasts
  JOURNAL   Biochem Biophys Res Commun 187 (1), 1-7 (1992)
   PUBMED   1520290
REFERENCE   10 (bases 1 to 3786)
  AUTHORS   Nishida,W., Kitami,Y., Abe,M. and Hiwada,K.
  TITLE     Gene cloning and nucleotide sequence of SM22 alpha from the chicken
            gizzard smooth muscle
  JOURNAL   Biochem Int 23 (4), 663-668 (1991)
   PUBMED   1872880
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ897303.1 and AP000892.4.
            
            On May 17, 2019 this sequence version replaced NM_003186.4.
            
            Summary: This gene encodes a shape change and transformation
            sensitive actin-binding protein which belongs to the calponin
            family. It is ubiquitously expressed in vascular and visceral
            smooth muscle, and is an early marker of smooth muscle
            differentiation. The encoded protein is thought to be involved in
            calcium-independent smooth muscle contraction. It acts as a tumor
            suppressor, and the loss of its expression is an early event in
            cell transformation and the development of some tumors, coinciding
            with cellular plasticity. The encoded protein has a domain
            architecture consisting of an N-terminal calponin homology (CH)
            domain and a C-terminal calponin-like (CLIK) domain. Mice with a
            knockout of the orthologous gene are viable and fertile but their
            vascular smooth muscle cells exhibit alterations in the
            distribution of the actin filament and changes in cytoskeletal
            organization. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.222280.1, M95787.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392951.9/ ENSP00000376678.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-708               BQ897303.1         49-756
            709-3786            AP000892.4         2870-5947
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..3786
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /note="transgelin"
                     /db_xref="GeneID:6876"
                     /db_xref="HGNC:HGNC:11553"
                     /db_xref="MIM:600818"
     exon            1..63
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /inference="alignment:Splign:2.1.0"
     exon            64..255
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..681
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /note="22 kDa actin-binding protein; smooth muscle protein
                     22-alpha; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="transgelin"
                     /protein_id="NP_003177.2"
                     /db_xref="CCDS:CCDS8381.1"
                     /db_xref="GeneID:6876"
                     /db_xref="HGNC:HGNC:11553"
                     /db_xref="MIM:600818"
                     /translation="MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGR
                     PDRGRLGFQVWLKNGVILSKLVNSLYPDGSKPVKVPENPPSMVFKQMEQVAQFLKAAE
                     DYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHK
                     REFTESQLQEGKHVIGLQMGSNRGASQAGMTGYGRPRQIIS"
     exon            256..433
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /inference="alignment:Splign:2.1.0"
     exon            434..536
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /inference="alignment:Splign:2.1.0"
     exon            537..3786
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1114
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /note="major polyA site"
     regulatory      1441..1446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /note="hexamer: AAGAAA"
     polyA_site      1447
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
     polyA_site      1473
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
     regulatory      1482..1487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /note="hexamer: ATTAAA"
     polyA_site      1501
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
     regulatory      3764..3769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
                     /note="hexamer: ATTAAA"
     polyA_site      3786
                     /gene="TAGLN"
                     /gene_synonym="SM22; SM22-alpha; SMCC; TAGLN1; TGLN;
                     WS3-10"
ORIGIN      
        1 atcctgtctg tccgaaccca gacacaagtc ttcactcctt cctgcgagcc ctgaggaagc
       61 cttctttccc cagacatggc caacaagggt ccttcctatg gcatgagccg cgaagtgcag
      121 tccaaaatcg agaagaagta tgacgaggag ctggaggagc ggctggtgga gtggatcata
      181 gtgcagtgtg gccctgatgt gggccgccca gaccgtgggc gcttgggctt ccaggtctgg
      241 ctgaagaatg gcgtgattct gagcaagctg gtgaacagcc tgtaccctga tggctccaag
      301 ccggtgaagg tgcccgagaa cccaccctcc atggtcttca agcagatgga gcaggtggct
      361 cagttcctga aggcggctga ggactatggg gtcatcaaga ctgacatgtt ccagactgtt
      421 gacctctttg aaggcaaaga catggcagca gtgcagagga ccctgatggc tttgggcagc
      481 ttggcagtga ccaagaatga tgggcactac cgtggagatc ccaactggtt tatgaagaaa
      541 gcgcaggagc ataagaggga attcacagag agccagctgc aggagggaaa gcatgtcatt
      601 ggccttcaga tgggcagcaa cagaggggcc tcccaggccg gcatgacagg ctacggacga
      661 cctcggcaga tcatcagtta gagcggagag ggctagccct gagcccggcc ctcccccagc
      721 tccttggctg cagccatccc gcttagcctg cctcacccac acccgtgtgg taccttcagc
      781 cctggccaag ctttgaggct ctgtcactga gcaatggtaa ctgcacctgg gcagctcctc
      841 cctgtgcccc cagcctcagc ccaacttctt acccgaaagc atcactgcct tggcccctcc
      901 ctcccggctg cccccatcac ctctactgtc tcctccctgg gctaagcagg ggagaagcgg
      961 gctgggggta gcctggatgt gggccaagtc cactgtcctc cttggcggca aaagcccatt
     1021 gaagaagaac cagcccagcc tgccccctat cttgtcctgg aatatttttg gggttggaac
     1081 tcaaaaaaaa aaaaaaaaaa tcaatctttt ctcaggcctg gctggcagag tttgatttgc
     1141 cctggtcact tttgttatgg tttcagatct gcgcagggga caggcaggca tctcgggctt
     1201 gtatgtgttc ctggctgccg tcctctgaag gacccttcat gtgccaagat tccaggacag
     1261 tcagattctt tcagaggaaa caggccatag aacaggagag tgaatcttgg ggaccgaagg
     1321 gaaaaaggag ccactcagca gcatgcagtg gctttgggga gacagaggaa aggagagatt
     1381 gcgagtggca gaattgtttg gaaggttttt attttggaaa agctgtgcag aaaaaaattc
     1441 aagaaaatgt tgctgtgaaa agagaaaaac aaatcttaag cattaaaaaa aattcaacca
     1501 actagctcag aagaggggag aaggccaagg ttgagagaag agtcacagcc tgtcaggcag
     1561 atagctggcc tccggcggga aggccatttc cctgagcact tgcagaggaa gacagggtgg
     1621 tggcaggact ggagtggcag tggctcccaa ggctctctcc tccaacatgt gcatctgcca
     1681 tctgctctgc agtcctgccg caggattccc tagtgaagca gctcaggcct gggggagccg
     1741 tgtgtatccc agctgtgccg gcagcatcat accaatcgtg ggggtggtga aggagccagg
     1801 ggttcattca tggttggttt ctgatcaggc atcttgggaa tggataatgg aggcagccgc
     1861 tttcaggaca ggcatgtcca ggggctcctc ccagcctcta ccccgaagtc ctcttcccaa
     1921 gtgaccccag tgatgtttcc attgagatgc gctcctggct atggcaggca ccttctcaac
     1981 ttatatgtgg gaaggggtcc cccatgcttg ggggacctag gcagctggct tggcccaaga
     2041 gagatgagga tggaggccaa accaaagggg ggcgccaatc ccctgtccaa caccttctca
     2101 ccaaaagctc ccgtttggct ggaggcagac cctgtggctc tgaccagact tctctggcag
     2161 caatcctcca ccatttgtat cttaagaagg ccctcaccct ctttgagtgg agtcagagga
     2221 tgcctcagat tccagatgta agcatcagaa ctgctttctg tcaagagctc cctaattttg
     2281 ggaaagaaga gcctgtccca cactgtcagg ccctgaggtc agcagatctg ctcctccttc
     2341 ccgtgcggta cgtctaggtg ctgatgaggg cagtccaggg cgctcttgtt ctgggacagg
     2401 ctccagtccc cttgctccag caggtctggg gcaaggaggt cagaggtggt gggagggccc
     2461 ctgctctctg tttccacttc gtctggatcc ttgctgctgc aaagtggcac tgattctagc
     2521 tctgtccctt cctccttggc tttccggctc cgatggggcc agtggcaggg tccactccta
     2581 caaacttgat tggaagccac attcctctgg ctcaaatata cttccagcat gtagtaaaca
     2641 gtccagaaga cggtgaaaca gcctaccagc accagggtct ggggccgagg caagcaccta
     2701 cgtgaggcac tgtttcccga agcctacagc ctttctcagc ccaggacaat gagctcagaa
     2761 agtctttttc cttctaggga ctgccttttt cacccaaact gttccctctg ccccctcccc
     2821 cgacaaggcc tgtcctgtaa gagttaggct agcagggtag gagcaatgct atgaaaccac
     2881 tgctgggacc caggtcttcc cagtccttca ccccgcaggt caccctgaga aacacactaa
     2941 gaactccatc ccccagacag gacagtccca tctacccgca gcttgagtct tggccctagt
     3001 accttgaggg tgttgggggt gatggtgtaa ccatcttcct caggaatttt cagccccggt
     3061 gggcagggca gggcgaagtc atcgtgcagg tattttccac tcatggcact ctctaacagc
     3121 ctgtggagaa gaaaagaggt gaggatgccc cactgactat ggttcctata ccatctgccc
     3181 tcacccattt tcctgcctta caggtgaaga cagggaagcc ccacagcaaa aaggagaggc
     3241 ccagactgtg agttcccagc ccggggctcc atctaccggc ttgacgctgg aactgggaag
     3301 cacagctggg gttcagaggg cgatgggctc cctaggcggc ccttcccctg tgactggcct
     3361 tccctctgac acatgcggct ttacccacct ttgtctgtcc ctgatgtcta ctgcactcca
     3421 cacagagcca tatagggtca gctgccattg ccggaggatg ccgacctgga atgactcatc
     3481 ccctagggca gcaaggggca gctcgtgagc cacgcccctc tgtcagccat gccagacagc
     3541 agaggccctg cccgccctgc cacccaaagg ggctcaggcc acatggtctg ctccaggagc
     3601 tgcctcactg tgtcccactg accccaggtt ctgcagaagg gcctcactgg gtgcccttag
     3661 ggatggaaag ggttgaaagg ctgtactcca aagcagagtc ttgcttttct ctcccgtatt
     3721 ttgggggttc agctgggatt agaaaaaaat gtctttccac caaattaaag aaagctttga
     3781 aaacca
//
