LOCUS       NM_006951               3259 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens TATA-box binding protein associated factor 5 (TAF5),
            transcript variant 1, mRNA.
ACCESSION   NM_006951
VERSION     NM_006951.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Gupta,K., Watson,A.A., Baptista,T., Scheer,E., Chambers,A.L.,
            Koehler,C., Zou,J., Obong-Ebong,I., Kandiah,E., Temblador,A.,
            Round,A., Forest,E., Man,P., Bieniossek,C., Laue,E.D., Lemke,E.A.,
            Rappsilber,J., Robinson,C.V., Devys,D., Tora,L. and Berger,I.
  TITLE     Architecture of TAF11/TAF13/TBP complex suggests novel regulation
            properties of general transcription factor TFIID
  JOURNAL   Elife 6, e30395 (2017)
   PUBMED   29111974
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3259)
  AUTHORS   Louder,R.K., He,Y., Lopez-Blanco,J.R., Fang,J., Chacon,P. and
            Nogales,E.
  TITLE     Structure of promoter-bound TFIID and model of human pre-initiation
            complex assembly
  JOURNAL   Nature 531 (7596), 604-609 (2016)
   PUBMED   27007846
  REMARK    Erratum:[Nature. 2016 Aug 4;536(7614):112. doi:
            10.1038/nature17984.. PMID: 27096372]
REFERENCE   4  (bases 1 to 3259)
  AUTHORS   Ba,X., Bacsi,A., Luo,J., Aguilera-Aguirre,L., Zeng,X., Radak,Z.,
            Brasier,A.R. and Boldogh,I.
  TITLE     8-oxoguanine DNA glycosylase-1 augments proinflammatory gene
            expression by facilitating the recruitment of site-specific
            transcription factors
  JOURNAL   J Immunol 192 (5), 2384-2394 (2014)
   PUBMED   24489103
  REMARK    GeneRIF: results show that nonproductive binding of OGG1 to 8-oxoG
            in promoter sequences could be an epigenetic mechanism to modulate
            gene expression for a prompt innate immune response.
REFERENCE   5  (bases 1 to 3259)
  AUTHORS   Bieniossek,C., Papai,G., Schaffitzel,C., Garzoni,F., Chaillet,M.,
            Scheer,E., Papadopoulos,P., Tora,L., Schultz,P. and Berger,I.
  TITLE     The architecture of human general transcription factor TFIID core
            complex
  JOURNAL   Nature 493 (7434), 699-702 (2013)
   PUBMED   23292512
REFERENCE   6  (bases 1 to 3259)
  AUTHORS   Bhattacharya,S., Takada,S. and Jacobson,R.H.
  TITLE     Structural analysis and dimerization potential of the human TAF5
            subunit of TFIID
  JOURNAL   Proc Natl Acad Sci U S A 104 (4), 1189-1194 (2007)
   PUBMED   17227857
  REMARK    GeneRIF: TAF5 protein demonstrates the ability of the N-terminal
            half of the TAF5 gene to form a flexible, extended dimer, a key
            property required for the assembly of the TFIID complex
REFERENCE   7  (bases 1 to 3259)
  AUTHORS   Tanese,N., Saluja,D., Vassallo,M.F., Chen,J.L. and Admon,A.
  TITLE     Molecular cloning and analysis of two subunits of the human TFIID
            complex: hTAFII130 and hTAFII100
  JOURNAL   Proc Natl Acad Sci U S A 93 (24), 13611-13616 (1996)
   PUBMED   8942982
REFERENCE   8  (bases 1 to 3259)
  AUTHORS   Zhou,Q. and Sharp,P.A.
  TITLE     Novel mechanism and factor for regulation by HIV-1 Tat
  JOURNAL   EMBO J 14 (2), 321-328 (1995)
   PUBMED   7835343
REFERENCE   9  (bases 1 to 3259)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   10 (bases 1 to 3259)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X95525.1, BC052268.1,
            BG290312.1 and AW136916.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006951.4.
            
            Summary: Initiation of transcription by RNA polymerase II requires
            the activities of more than 70 polypeptides. The protein that
            coordinates these activities is transcription factor IID (TFIID),
            which binds to the core promoter to position the polymerase
            properly, serves as the scaffold for assembly of the remainder of
            the transcription complex, and acts as a channel for regulatory
            signals. TFIID is composed of the TATA-binding protein (TBP) and a
            group of evolutionarily conserved proteins known as TBP-associated
            factors or TAFs. TAFs may participate in basal transcription, serve
            as coactivators, function in promoter recognition or modify general
            transcription factors (GTFs) to facilitate complex assembly and
            transcription initiation. This gene encodes an integral subunit of
            TFIID associated with all transcriptionally competent forms of that
            complex. This subunit interacts strongly with two TFIID subunits
            that show similarity to histones H3 and H4, and it may participate
            in forming a nucleosome-like core in the TFIID complex. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.276184.1, BC052268.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369839.4/ ENSP00000358854.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-389               X95525.1           10-398
            390-2373            BC052268.1         391-2374
            2374-2714           BG290312.1         220-560
            2715-3243           BC052268.1         2716-3244
            3244-3259           AW136916.1         16-31               c
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.33"
     gene            1..3259
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="TATA-box binding protein associated factor 5"
                     /db_xref="GeneID:6877"
                     /db_xref="HGNC:HGNC:11539"
                     /db_xref="MIM:601787"
     exon            1..573
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..2417
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="isoform 1 is encoded by transcript variant 1; TATA
                     box binding protein (TBP)-associated factor, RNA
                     polymerase II, D, 100kD; TATA box binding protein
                     (TBP)-associated factor 2D; transcription initiation
                     factor TFIID 100 kD subunit; transcription initiation
                     factor TFIID subunit 5; transcription initiation factor
                     TFIID 100 kDa subunit; TAF5 RNA polymerase II, TATA box
                     binding protein (TBP)-associated factor, 100kDa"
                     /codon_start=1
                     /product="transcription initiation factor TFIID subunit 5
                     isoform 1"
                     /protein_id="NP_008882.2"
                     /db_xref="CCDS:CCDS7547.1"
                     /db_xref="GeneID:6877"
                     /db_xref="HGNC:HGNC:11539"
                     /db_xref="MIM:601787"
                     /translation="MAALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGP
                     NGGGGNVAASSSTGGDGGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQ
                     FLRQSKLREAEEALRREAGLLEEAVAGSGAPGEVDSAGAEVTSALLSRVTASAPGPAA
                     PDPPGTGASGATVVSGSASGPAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYY
                     SGLKHFIECSLDCHRAELSQLFYPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQ
                     DDLRVLSSLTKKEHMKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQ
                     EHLYIDIFDGMPRSKQQIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEE
                     GENEEGKPKKKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSDKLDKIMNMKETTKR
                     VRLGPDCLPSICFYTFLNAYQGLTAVDVTDDSSLIAGGFADSTVRVWSVTPKKLRSVK
                     QASDLSLIDKESDDVLERIMDEKTASELKILYGHSGPVYGASFSPDRNYLLSSSEDGT
                     VRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYFVSGGHDRVARLWATDHYQPLRIFA
                     GHLADVNCTRFHPNSNYVATGSADRTVRLWDVLNGNCVRIFTGHKGPIHSLTFSPNGR
                     FLATGATDGRVLLWDIGHGLMVGELKGHTDTVCSLRFSRDGEILASGSMDNTVRLWDA
                     IKAFEDLETDDFTTATGHINLPENSQELLLGTYMTKSTPVVHLHFTRRNLVLAAGAYS
                     PQ"
     exon            574..811
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            812..1127
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1291
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1427
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1548
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1678
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1843
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..2021
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2199
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..3259
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2413..2418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="hexamer: AATAAA"
     polyA_site      2434
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="major polyA site"
     regulatory      3228..3233
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="hexamer: AATAAA"
     polyA_site      3259
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="major polyA site"
ORIGIN      
        1 ggctcagccg caagatggcg gcgctggcgg aggagcagac ggaggtggcg gtcaagctag
       61 agcctgaggg accgccaacg ctgctacctc cgcaggcggg ggacggcgca ggcgagggta
      121 gcggcggcac taccaacaac ggccccaacg gcggcggcgg gaacgttgcg gcgtcgtcgt
      181 ccactggcgg ggatggcggg acccccaagc ccacggtggc tgtctccgcc gctgccccgg
      241 cgggggcggc cccggtgccc gccgctgctc cggacgccgg cgctccgcat gaccgacaga
      301 ctctactggc cgtgctgcag ttcctacggc agagcaaact ccgcgaggcc gaagaggcgc
      361 tgcgccgtga ggccgggctg ctggaggagg cagtggcggg ctccggagcc ccgggagagg
      421 tggacagcgc cggcgctgag gtgaccagcg cgcttctcag ccgggtgacc gcctcggccc
      481 ctggccctgc ggcccccgac cctccgggca ctggcgcttc gggggccacg gtcgtctcag
      541 gttcagcctc aggtcctgcg gctccgggta aagttggaag tgttgctgtg gaagaccagc
      601 cagatgtcag tgccgtgttg tcagcctaca accaacaagg agatcccaca atgtatgaag
      661 aatactatag tggactgaaa cacttcattg aatgttccct ggactgccat cgggcagagt
      721 tgtcccaact tttttatcct ctgtttgtgc acatgtactt ggagctagtc tacaatcaac
      781 atgagaatga agcaaagtca ttctttgaga agttccatgg agatcaggaa tgttattacc
      841 aggatgacct acgagtatta tctagtctta ccaaaaagga acacatgaaa gggaatgaga
      901 ccatgttgga ttttcgaaca agtaaatttg ttctgcgtat ttcccgtgac tcgtaccaac
      961 tcttgaagag gcatcttcag gagaaacaga acaatcagat atggaacata gttcaggagc
     1021 acctctacat tgacatcttt gatgggatgc cgcgtagtaa gcaacagata gatgcgatgg
     1081 tgggaagttt ggcaggagag gctaaacgag aggcaaacaa atcaaaggta ttttttggtt
     1141 tattaaaaga accagaaatt gaggtacctt tggatgacga ggatgaagag ggagaaaatg
     1201 aagaaggaaa acctaaaaag aagaagccta aaaaagatag tattggatcc aaaagcaaaa
     1261 aacaagatcc caatgctcca cctcagaaca gaatccctct tcctgagttg aaagattcag
     1321 ataagttgga taagataatg aatatgaaag aaaccaccaa acgagtgcgc cttgggccgg
     1381 actgcttacc ctccatttgt ttctatacat ttctcaatgc ttaccagggt ctcactgcag
     1441 tggatgtcac tgatgattct agtctgattg ctggaggttt tgcagattca actgtcagag
     1501 tgtggtcggt aacacccaaa aagcttcgta gtgtcaaaca agcatcagat cttagtctta
     1561 tagacaaaga atcagatgat gtcttagaaa gaatcatgga tgagaaaaca gcaagtgagt
     1621 tgaagatttt gtatggtcac agtgggcctg tctacggagc cagcttcagt ccggatagga
     1681 actatctgct ttcctcttca gaggacggaa ctgttagatt gtggagcctt caaacattta
     1741 cttgtttggt gggatataaa ggacacaact atccagtatg ggacacacaa ttttctccat
     1801 atggatatta ttttgtgtca gggggccatg accgagtagc tcggctctgg gctacagacc
     1861 actatcagcc tttaagaata tttgccggcc atcttgctga tgtgaattgt accagattcc
     1921 atccaaattc taattatgtt gctacgggct ctgcagacag aactgtgcgg ctctgggacg
     1981 tcctgaatgg taactgtgta aggatcttca ctggacacaa gggaccaatt cattccttga
     2041 cattttctcc caatgggaga ttcctggcta caggagcaac agatggcaga gtgcttcttt
     2101 gggatattgg acatggtttg atggttggag aattaaaagg ccacactgat acagtctgtt
     2161 cacttaggtt tagtagagat ggtgaaattt tggcatcagg ttcaatggat aatacagttc
     2221 gattatggga tgctatcaaa gcctttgaag atttagagac cgatgacttt actacagcca
     2281 ctgggcatat aaatttacct gagaattcac aggagttatt gttgggaaca tatatgacca
     2341 aatcaacacc agttgtacac cttcatttta ctcgaagaaa cctggttcta gctgcaggag
     2401 cttatagtcc acaataaacc atcggtatta aagacctttt ggaagctact gtttttaaaa
     2461 agggagacta aaagcaaata cctcagtgat taatatttaa gctacagaga atgtttttgt
     2521 ctatatggat ctggaagtat gctgcttgga aaaatctgaa caggacagtt ccacgtttct
     2581 atagcaacca catttgacta atttccgtta gttgaataag aggtattatg atcatggagg
     2641 ggacatttat ggtgctttgg attgtgtgga aactatgcat tttctgttca aatgctattt
     2701 taatttatta catttagaaa aaaagttgat ttcaataatt catcctgctt caagattcaa
     2761 attcagaaat atactatcat cttgaatttt agctgaagaa tcctatgagc atgtatgttt
     2821 ctgctgtaaa aacgtagtta ctgtatggca ctcaaaaact atgttaaatg atccactaac
     2881 tttttttttc ttggcccatg attaatggaa tgtatgtaac taggtagggt tcctttctta
     2941 gatctagagg aagtacagcc acccactgac atctgaattt atatacctgt tgagttttga
     3001 gtgcacccaa acactcgata aaccaggtga agaaatttag cttccatgtt ctacttcagc
     3061 taaaacagct acatacaacc tagtacactt gaagtcagac agacatttca gttgcttacc
     3121 tccagtactg agccttgctt tgggaaacta aaagatttag accaagtcac tgccagtttt
     3181 tgcctttgtt gcattttgta cagtttttat atttttgata tcttgtaaat aaagacaacc
     3241 agcttttcca ggttcataa
//
