LOCUS       NM_005644               1335 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens TATA-box binding protein associated factor 12 (TAF12),
            transcript variant 2, mRNA.
ACCESSION   NM_005644
VERSION     NM_005644.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1335)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1335)
  AUTHORS   Xu,Y., Milazzo,J.P., Somerville,T.D.D., Tarumoto,Y., Huang,Y.H.,
            Ostrander,E.L., Wilkinson,J.E., Challen,G.A. and Vakoc,C.R.
  TITLE     A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute
            Myeloid Leukemia
  JOURNAL   Cancer Cell 33 (1), 13-28 (2018)
   PUBMED   29316427
  REMARK    GeneRIF: Ectopic expression of the TAF4 histone-fold fragment can
            efficiently squelch TAF12 in cells
REFERENCE   5  (bases 1 to 1335)
  AUTHORS   Gupta,K., Watson,A.A., Baptista,T., Scheer,E., Chambers,A.L.,
            Koehler,C., Zou,J., Obong-Ebong,I., Kandiah,E., Temblador,A.,
            Round,A., Forest,E., Man,P., Bieniossek,C., Laue,E.D., Lemke,E.A.,
            Rappsilber,J., Robinson,C.V., Devys,D., Tora,L. and Berger,I.
  TITLE     Architecture of TAF11/TAF13/TBP complex suggests novel regulation
            properties of general transcription factor TFIID
  JOURNAL   Elife 6, e30395 (2017)
   PUBMED   29111974
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1335)
  AUTHORS   Hisatake,K., Ohta,T., Takada,R., Guermah,M., Horikoshi,M.,
            Nakatani,Y. and Roeder,R.G.
  TITLE     Evolutionary conservation of human
            TATA-binding-polypeptide-associated factors TAFII31 and TAFII80 and
            interactions of TAFII80 with other TAFs and with general
            transcription factors
  JOURNAL   Proc Natl Acad Sci U S A 92 (18), 8195-8199 (1995)
   PUBMED   7667268
REFERENCE   7  (bases 1 to 1335)
  AUTHORS   Mengus,G., May,M., Jacq,X., Staub,A., Tora,L., Chambon,P. and
            Davidson,I.
  TITLE     Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28:
            three subunits of the human transcription factor TFIID
  JOURNAL   EMBO J 14 (7), 1520-1531 (1995)
   PUBMED   7729427
REFERENCE   8  (bases 1 to 1335)
  AUTHORS   Zhou,Q. and Sharp,P.A.
  TITLE     Novel mechanism and factor for regulation by HIV-1 Tat
  JOURNAL   EMBO J 14 (2), 321-328 (1995)
   PUBMED   7835343
REFERENCE   9  (bases 1 to 1335)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   10 (bases 1 to 1335)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX095156.1, U57693.1,
            BM676557.1 and AL513497.21.
            
            On Jun 2, 2019 this sequence version replaced NM_005644.3.
            
            Summary: Control of transcription by RNA polymerase II involves the
            basal transcription machinery which is a collection of proteins.
            These proteins with RNA polymerase II, assemble into complexes
            which are modulated by transactivator proteins that bind to
            cis-regulatory elements located adjacent to the transcription start
            site. Some modulators interact directly with the basal complex,
            whereas others may act as bridging proteins linking transactivators
            to the basal transcription factors. Some of these associated
            factors are weakly attached while others are tightly associated
            with TBP in the TFIID complex. Among the latter are the TAF
            proteins. Different TAFs are predicted to mediate the function of
            distinct transcriptional activators for a variety of gene promoters
            and RNA polymerases. TAF12 interacts directly with TBP as well as
            with TAF2I. Two transcript variants encoding the same protein have
            been found for this gene. [provided by RefSeq, Sep 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U57693.1, BM904501.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373824.9/ ENSP00000362930.4
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                BX095156.1         26-60
            36-1005             U57693.1           1-970
            1006-1089           BM676557.1         16-99               c
            1090-1335           AL513497.21        102346-102591       c
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..1335
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /note="TATA-box binding protein associated factor 12"
                     /db_xref="GeneID:6883"
                     /db_xref="HGNC:HGNC:11545"
                     /db_xref="MIM:600773"
     exon            1..76
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /inference="alignment:Splign:2.1.0"
     exon            77..328
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..646
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /note="isoform 1 is encoded by transcript variant 2;
                     transcription initiation factor TFIID 20/15 kDa subunits;
                     TATA box binding protein (TBP)-associated factor, RNA
                     polymerase II, J, 20kD; transcription initiation factor
                     TFIID subunit 12; TAFII20/TAFII15; TAFII-20/TAFII-15;
                     TAF12 RNA polymerase II, TATA box binding protein
                     (TBP)-associated factor, 20kDa"
                     /codon_start=1
                     /product="transcription initiation factor TFIID subunit 12
                     isoform 1"
                     /protein_id="NP_005635.1"
                     /db_xref="CCDS:CCDS326.1"
                     /db_xref="GeneID:6883"
                     /db_xref="HGNC:HGNC:11545"
                     /db_xref="MIM:600773"
                     /translation="MNQFGPSALINLSNFSSIKPEPASTPPQGSMANSTAVVKIPGTP
                     GAGGRLSPENNQVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQL
                     ARHRKSSTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKTTK
                     K"
     exon            329..406
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /inference="alignment:Splign:2.1.0"
     exon            407..521
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /inference="alignment:Splign:2.1.0"
     exon            522..610
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /inference="alignment:Splign:2.1.0"
     exon            611..1335
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1067..1072
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /note="hexamer: AATAAA"
     polyA_site      1089
                     /gene="TAF12"
                     /gene_synonym="TAF2J; TAFII20"
                     /note="major polyA site"
ORIGIN      
        1 agtcggaccg gctgctgaga cgaacgcttc actggggcag tctctgcata tcatggggag
       61 atagacgctg ctgcctttaa ttggccttgg tcctcacagc tccaaaaaga aacaggatct
      121 cgataagctc tatgagctga agtccaaagc tcggcagatt atgaaccagt ttggcccctc
      181 agccctaatc aacctctcca atttctcatc cataaaaccg gaaccagcca gcacccctcc
      241 acaaggctcc atggccaata gtactgcagt ggtaaagata ccaggcactc ctggggcagg
      301 aggtcgtctt agccctgaaa acaatcaggt attgaccaag aagaaattac aggacttagt
      361 aagagaagtg gatcctaatg agcagttgga tgaagatgtg gaggagatgc tgctgcagat
      421 tgctgatgat tttatcgaga gtgtggtgac agcagcctgt cagcttgcgc ggcatcgcaa
      481 gtctagcacc ctggaggtga aagatgtcca gctgcattta gagcgccagt ggaacatgtg
      541 gatcccagga tttggctctg aagaaatccg accctacaaa aaagcttgca ccacagaagc
      601 tcacaaacag agaatggcat tgatccggaa aacaaccaag aaataacaca cggaaaggtc
      661 agggaatgga cagcaatgta tttggagata cttgagctga gaactcagcc atctcatcct
      721 tggatttttt tttttaatgc tttacagaga agcatatatt ttttattaac agtgcagcaa
      781 tatctataat gactgagagg atctgccaaa agaataaagc ctcctacccc aacttccggt
      841 cccttttccc tgccatctca aagaggagca atgtatcttc cagagaagat tttatttgtg
      901 gtttattata taagtgactg aatatgggac aaagcattat ggtctttttg ggtaagacag
      961 tattagcagg atttgtaaag ggttttgttt ccttctccct tccccttttc cctgtacttt
     1021 gtaatgtcag tgtttatatg aatatgactt tcatttgctt ttccagaata aagaagttat
     1081 taatcgaaag acagggtacc tttgaaatcc cagagggagt caggacaagc aagcctggat
     1141 tgtagctctc tagtcagcag gtggcagcat cgttattcat tttggtgttt gagaaaaact
     1201 cagggatttt ccatcatttt accccaaggg aggccataac atcagcagga gtttgagtcc
     1261 agcttctcgg ccataagaat tgagtagggt gaccttctct cacggccctc aactgaagag
     1321 gcatccttgt tttgg
//
