LOCUS       NM_206862               9704 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens transforming acidic coiled-coil containing protein 2
            (TACC2), transcript variant 1, mRNA.
ACCESSION   NM_206862
VERSION     NM_206862.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Ito,S., Takuwa,H., Kakehi,S., Someya,Y., Kaga,H., Kumahashi,N.,
            Kuwata,S., Wakatsuki,T., Kadowaki,M., Yamamoto,S., Abe,T.,
            Takeda,M., Ishikawa,Y., Liu,X., Otomo,N., Suetsugu,H., Koike,Y.,
            Hikino,K., Tomizuka,K., Momozawa,Y., Ozaki,K., Isomura,M.,
            Nabika,T., Kaneko,H., Ishijima,M., Kawamori,R., Watada,H.,
            Tamura,Y., Uchio,Y., Ikegawa,S. and Terao,C.
  TITLE     A genome-wide association study identifies a locus associated with
            knee extension strength in older Japanese individuals
  JOURNAL   Commun Biol 7 (1), 513 (2024)
   PUBMED   38769351
  REMARK    GeneRIF: A genome-wide association study identifies a locus
            associated with knee extension strength in older Japanese
            individuals.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Ito,Y., Terao,Y., Noma,S., Tagami,M., Yoshida,E., Hayashizaki,Y.,
            Itoh,M. and Kawaji,H.
  TITLE     Nanopore sequencing reveals TACC2 locus complexity and diversity of
            isoforms transcribed from an intronic promoter
  JOURNAL   Sci Rep 11 (1), 9355 (2021)
   PUBMED   33931666
  REMARK    GeneRIF: Nanopore sequencing reveals TACC2 locus complexity and
            diversity of isoforms transcribed from an intronic promoter.
            Erratum:[Sci Rep. 2021 Aug 13;11(1):16835. doi:
            10.1038/s41598-021-96196-9.. PMID: 34389798]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9704)
  AUTHORS   Mallampalli,R.K., Li,X., Jang,J.H., Kaminski,T., Hoji,A., Coon,T.,
            Chandra,D., Welty,S., Teng,Y., Sembrat,J., Rojas,M., Zhao,Y.,
            Lafyatis,R., Zou,C., Sciurba,F., Sundd,P., Lan,L. and Nyunoya,T.
  TITLE     Cigarette smoke exposure enhances transforming acidic
            coiled-coil-containing protein 2 turnover and thereby promotes
            emphysema
  JOURNAL   JCI Insight 5 (2), 125895 (2020)
   PUBMED   31996486
  REMARK    GeneRIF: Cigarette smoke exposure enhances transforming acidic
            coiled-coil-containing protein 2 turnover and thereby promotes
            emphysema.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9704)
  AUTHORS   Shakya,M., Zhou,A., Dai,D., Zhong,Q., Zhou,Z., Zhang,Y., Li,X.,
            Bholee,A.K. and Chen,M.
  TITLE     High expression of TACC2 in hepatocellular carcinoma is associated
            with poor prognosis
  JOURNAL   Cancer Biomark 22 (4), 611-619 (2018)
   PUBMED   29843208
  REMARK    GeneRIF: This finding suggests that TACC2 may be a useful tool as a
            candidate biomarker to predict the recurrence and prognosis of
            hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 9704)
  AUTHORS   Yoshida,E., Terao,Y., Hayashi,N., Mogushi,K., Arakawa,A.,
            Tanaka,Y., Ito,Y., Ohmiya,H., Hayashizaki,Y., Takeda,S., Itoh,M.
            and Kawaji,H.
  TITLE     Promoter-level transcriptome in primary lesions of endometrial
            cancer identified biomarkers associated with lymph node metastasis
  JOURNAL   Sci Rep 7 (1), 14160 (2017)
   PUBMED   29074988
  REMARK    GeneRIF: TACC2 protein is expressed mainly in the nucleus of the
            endometrial cancer cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9704)
  AUTHORS   Gergely,F., Karlsson,C., Still,I., Cowell,J., Kilmartin,J. and
            Raff,J.W.
  TITLE     The TACC domain identifies a family of centrosomal proteins that
            can interact with microtubules
  JOURNAL   Proc Natl Acad Sci U S A 97 (26), 14352-14357 (2000)
   PUBMED   11121038
REFERENCE   7  (bases 1 to 9704)
  AUTHORS   Chen,H.M., Schmeichel,K.L., Mian,I.S., Lelievre,S., Petersen,O.W.
            and Bissell,M.J.
  TITLE     AZU-1: a candidate breast tumor suppressor and biomarker for tumor
            progression
  JOURNAL   Mol Biol Cell 11 (4), 1357-1367 (2000)
   PUBMED   10749935
REFERENCE   8  (bases 1 to 9704)
  AUTHORS   Gergely,F., Kidd,D., Jeffers,K., Wakefield,J.G. and Raff,J.W.
  TITLE     D-TACC: a novel centrosomal protein required for normal spindle
            function in the early Drosophila embryo
  JOURNAL   EMBO J 19 (2), 241-252 (2000)
   PUBMED   10637228
REFERENCE   9  (bases 1 to 9704)
  AUTHORS   Still,I.H., Hamilton,M., Vince,P., Wolfman,A. and Cowell,J.K.
  TITLE     Cloning of TACC1, an embryonically expressed, potentially
            transforming coiled coil containing gene, from the 8p11 breast
            cancer amplicon
  JOURNAL   Oncogene 18 (27), 4032-4038 (1999)
   PUBMED   10435627
REFERENCE   10 (bases 1 to 9704)
  AUTHORS   Still,I.H., Vince,P. and Cowell,J.K.
  TITLE     The third member of the transforming acidic coiled coil-containing
            gene family, TACC3, maps in 4p16, close to translocation
            breakpoints in multiple myeloma, and is upregulated in various
            cancer cell lines
  JOURNAL   Genomics 58 (2), 165-170 (1999)
   PUBMED   10366448
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL731566.7, AC063960.10 and
            AL135793.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_206862.3.
            
            Summary: Transforming acidic coiled-coil proteins are a conserved
            family of centrosome- and microtubule-interacting proteins that are
            implicated in cancer. This gene encodes a protein that concentrates
            at centrosomes throughout the cell cycle. This gene lies within a
            chromosomal region associated with tumorigenesis. Expression of
            this gene is induced by erythropoietin and is thought to affect the
            progression of breast tumors. Several transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Isoform a has also
            been called the 'long' isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF528099.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369005.6/ ENSP00000358001.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-326               AL731566.7         98120-98445
            327-404             AC063960.10        167629-167706       c
            405-517             AC063960.10        139093-139205       c
            518-5830            AC063960.10        101684-106996       c
            5831-5944           AC063960.10        101052-101165       c
            5945-6070           AC063960.10        56909-57034         c
            6071-6205           AC063960.10        45937-46071         c
            6206-6342           AL135793.13        37085-37221
            6343-7654           AL135793.13        52442-53753
            7655-7715           AL135793.13        57436-57496
            7716-7917           AL135793.13        58672-58873
            7918-7979           AL135793.13        66771-66832
            7980-8095           AL135793.13        68411-68526
            8096-8267           AL135793.13        69882-70053
            8268-8408           AL135793.13        71391-71531
            8409-8498           AL135793.13        72396-72485
            8499-8642           AL135793.13        79440-79583
            8643-8719           AL135793.13        80006-80082
            8720-8763           AL135793.13        84003-84046
            8764-8924           AL135793.13        90688-90848
            8925-9031           AL135793.13        91095-91201
            9032-9152           AL135793.13        91589-91709
            9153-9704           AL135793.13        96036-96587
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.13"
     gene            1..9704
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /note="transforming acidic coiled-coil containing protein
                     2"
                     /db_xref="GeneID:10579"
                     /db_xref="HGNC:HGNC:11523"
                     /db_xref="MIM:605302"
     exon            1..326
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            327..404
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     CDS             372..9218
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /note="isoform a is encoded by transcript variant 1; anti
                     zuai-1"
                     /codon_start=1
                     /product="transforming acidic coiled-coil-containing
                     protein 2 isoform a"
                     /protein_id="NP_996744.4"
                     /db_xref="CCDS:CCDS7626.1"
                     /db_xref="GeneID:10579"
                     /db_xref="HGNC:HGNC:11523"
                     /db_xref="MIM:605302"
                     /translation="MGNENSTSDNQRTLSAQTPRSAQPPGNSQNIKRKQQDTPGSPDH
                     RDASSIGSVGLGGFCTASESSASLDPCLVSPEVTEPRKDPQGARGPEGSLLPSPPPSQ
                     EREHPSSSMPFAECPPEGCLASPAAAPEDGPQTQSPRREPAPNAPGDIAAAFPAERDS
                     STPYQEIAAVPSAGRERQPKEEGQKSSFSFSSGIDQSPGMSPVPLREPMKAPLCGEGD
                     QPGGFESQEKEAAGGFPPAESRQGVASVQVTPEAPAAAQQGTESSAVLEKSPLKPMAP
                     IPQDPAPRASDRERGQGEAPPQYLTDDLEFLRACHLPRSNSGAAPEAEVNAASQESCQ
                     QPVGAYLPHAELPWGLPSPALVPEAGGSGKEALDTIDVQGHPQTGMRGTKPNQVVCVA
                     AGGQPEGGLPVSPEPSLLTPTEEAHPASSLASFPAAQIPIAVEEPGSSSRESVSKAGM
                     PVSADAAKEVVDAGLVGLERQVSDLGSKGEHPEGDPGEVPAPSPQERGEHLNTEQSHE
                     VQPGVPPPPLPKEQSHEVQPGAPPPPLPKAPSESARGPPGPTDGAKVHEDSTSPAVAK
                     EGSRSPGDSPGGKEEAPEPPDGGDPGNLQGEDSQAFSSKRDPEVGKDELSKPSSDAES
                     RDHPSSHSAQPPRKGGAGHTDGPHSQTAEADASGLPHKLGEEDPVLPPVPDGAGEPTV
                     PEGAIWEGSGLQPKCPDTLQSREGLGRMESFLTLESEKSDFPPTPVAEVAPKAQEGES
                     TLEIRKMGSCDGEGLLTSPDQPRGPACDASRQEFHAGVPHPPQGENLAADLGLTALIL
                     DQDQQGIPSCPGEGWIRGAASEWPLLSSEKHLQPSQAQPETSIFDVLKEQAQPPENGK
                     ETSPSHPGFKDQGADSSQIHVPVEPQEDNNLPTHGGQEQALGSELQSQLPKGTLSDTP
                     TSSPTDMVWESSLTEESELSAPTRQKLPALGEKRPEGACGDGQSSRVSPPAADVLKDF
                     SLAGNFSRKETCCTGQGPNKSQQALADALEEGSQHEEACQRHPGASEAADGCSPLWGL
                     SKREMASGNTGEAPPCQPDSVALLDAVPCLPALAPASPGVTPTQDAPETEACDETQEG
                     RQQPVPAPQQKMECWATSDAESPKLLASFPSAGEQGGEAGAAETGGSAGAGDPGKQQA
                     PEKPGEATLSCGLLQTEHCLTSGEEASTSALRESCQAEHPMASCQDALLPARELGGIP
                     RSTMDFSTHQAVPDPKELLLSGPPEVAAPDTPYLHVDSAAQRGAEDSGVKAVSSADPR
                     APGESPCPVGEPPLALENAASLKLFAGSLAPLLQPGAAGGEIPAVQASSGSPKARTTE
                     GPVDSMPCLDRMPLLAKGKQATGEEKAATAPGAGAKASGEGMAGDAAGETEGSMERMG
                     EPSQDPKQGTSGGVDTSSEQIATLTGFPDFREHIAKIFEKPVLGALATPGEKAGAGRS
                     AVGKDLTRPLGPEKLLDGPPGVDVTLLPAPPARLQVEKKQQLAGEAEISHLALQDPAS
                     DKLLGPAGLTWERNLPGAGVGKEMAGVPPTLREDERPEGPGAAWPGLEGQAYSQLERS
                     RQELASGLPSPAATQELPVERAAAFQVAPHSHGEEAVAQDRIPSGKQHQETSACDSPH
                     GEDGPGDFAHTGVPGHVPRSTCAPSPQREVLTVPEANSEPWTLDTLGGERRPGVTAGI
                     LEMRNALGNQSTPAPPTGEVADTPLEPGKVAGAAGEAEGDITLSTAETQACASGDLPE
                     AGTTRTFSVVAGDLVLPGSCQDPACSDKAPGMEGTAALHGDSPARPQQAKEQPGPERP
                     IPAGDGKVCVSSPPEPDETHDPKLQHLAPEELHTDRESPRPGPSMLPSVPKKDAPRVM
                     DKVTSDETRGAEGTESSPVADDIIQPAAPADLESPTLAASSYHGDVVGQVSTDLIAQS
                     ISPAAAHAGLPPSAAEHIVSPSAPAGDRVEASTPSCPDPAKDLSRSSDSEEAFETPES
                     TTPVKAPPAPPPPPPEVIPEPEVSTQPPPEEPGCGSETVPVPDGPRSDSVEGSPFRPP
                     SHSFSAVFDEDKPIASSGTYNLDFDNIELVDTFQTLEPRASDAKNQEGKVNTRRKSTD
                     SVPISKSTLSRSLSLQASDFDGASSSGNPEAVALAPDAYSTGSSSASSTLKRTKKPRP
                     PSLKKKQTTKKPTETPPVKETQQEPDEESLVPSGENLASETKTESAKTEGPSPALLEE
                     TPLEPAVGPKAACPLDSESAEGVVPPASGGGRVQNSPPVGRKTLPLTTAPEAGEVTPS
                     DSGGQEDSPAKGLSVRLEFDYSEDKSSWDNQQENPPPTKKIGKKPVAKMPLRRPKMKK
                     TPEKLDNTPASPPRSPAEPNDIPIAKGTYTFDIDKWDDPNFNPFSSTSKMQESPKLPQ
                     QSYNFDPDTCDESVDPFKTSSKTPSSPSKSPASFEIPASAMEANGVDGDGLNKPAKKK
                     KTPLKTDTFRVKKSPKRSPLSDPPSQDPTPAATPETPPVISAVVHATDEEKLAVTNQK
                     WTCMTVDLEADKQDYPQPSDLSTFVNETKFSSPTEELDYRNSYEIEYMEKIGSSLPQD
                     DDAPKKQALYLMFDTSQESPVKSSPVRMSESPTPCSGSSFEETEALVNTAAKNQHPVP
                     RGLAPNQESHLQVPEKSSQKELEAMGLGTPSEAIEITAPEGSFASADALLSRLAHPVS
                     LCGALDYLEPDLAEKNPPLFAQKLQEELEFAIMRIEALKLARQIALASRSHQDAKREA
                     AHPTDVSISKTALYSRIGTAEVEKPAGLLFQQPDLDSALQIARAEIITKEREVSEWKD
                     KYEESRREVMEMRKIVAEYEKTIAQMIEDEQREKSVSHQTVQQLVLEKEQALADLNSV
                     EKSLADLFRRYEKMKEVLEGFRKNEEVLKRCAQEYLSRVKKEEQRYQALKVHAEEKLD
                     RANAEIAQVRGKAQQEQAAHQASLRKEQLRVDALERTLEQKNKEIEELTKICDELIAK
                     MGKS"
     exon            405..517
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            518..5830
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            5831..5944
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            5945..6070
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            6071..6205
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            6206..6342
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            6343..7654
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            7655..7715
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            7716..7917
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            7918..7979
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            7980..8095
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8096..8267
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8268..8408
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8409..8498
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8499..8642
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8643..8719
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8720..8763
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8764..8924
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            8925..9031
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            9032..9152
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     exon            9153..9704
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9346..9351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /note="hexamer: AATAAA"
     polyA_site      9373
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
     regulatory      9676..9681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /note="hexamer: AATAAA"
     polyA_site      9704
                     /gene="TACC2"
                     /gene_synonym="AZU-1; ECTACC"
                     /note="major polyA site"
ORIGIN      
        1 ataagtggac agcctgctcc aagggaagga tcaggagaga agaaacgcaa atcccagaac
       61 cgtgccaaca tataaaaccc cacattaagg gttgtacagt gcactgggat ttctcaagtc
      121 acccgcttgg tcctcttcca agtatacttt acttcctttc attcctctct aaaacttttt
      181 taaaaacttt cactcctgct ctaaaagtta tcttggtttc ttactctacc ttatgcccct
      241 tgggcgaatt ttttcctctg aggagggaag aatagagttg ctgctgcaga cacatcagat
      301 tccctactgg taacagctgg agtgcgtcac ctctgacaaa attctgggga cgctgggaac
      361 actgaatcaa catgggcaat gagaacagca cctcggacaa ccagaggact ttatcagctc
      421 agactccaag gtccgcgcag ccacccggga acagtcagaa tataaaaagg aagcagcagg
      481 acacgcccgg aagccctgac cacagagacg cgtccagcat tggcagcgtt gggcttggag
      541 gcttctgcac cgcttctgag agttctgcca gcctggatcc atgccttgtg tccccagagg
      601 tgactgagcc aaggaaggac ccacagggag ccagggggcc agaaggttct ttgctgccca
      661 gcccaccacc gtcccaggag cgagagcacc cctcgtcctc catgcccttt gccgagtgtc
      721 ccccggaagg ttgcttggca agtccagcag cggcacctga agatggtcct cagactcagt
      781 ctcccaggag ggaacctgcc ccaaatgccc caggagacat cgcggcggca tttcccgctg
      841 agagggacag ctctactcca taccaagaga ttgctgccgt ccccagtgct ggaagagaga
      901 gacagccgaa ggaagaagga cagaagtcct ccttctcctt ctccagtggc atcgaccagt
      961 cacctggaat gtcgccagta cccctcagag agccaatgaa ggcaccgctg tgtggagagg
     1021 gggaccagcc tggtggtttt gagtcccaag agaaagaggc tgcaggtggc tttccccctg
     1081 cagagtccag gcagggggtg gcttctgtgc aagtgacccc tgaggcccct gctgcagccc
     1141 agcagggcac agaaagctca gcggtcttgg agaagtcccc cctaaaaccc atggccccga
     1201 tcccacaaga tccagcccca agagcctcag acagagaaag aggccaaggg gaggcgccgc
     1261 ctcagtattt aacagatgac ttggaattcc tcagggcctg ccatctccct aggagcaatt
     1321 caggggctgc cccagaagca gaagtgaatg ccgcttccca ggagagctgc cagcagccag
     1381 tgggagcata tctgccgcac gcagagctgc cctggggctt gccaagtcct gccctggtgc
     1441 cagaggctgg gggctctggg aaggaggctc tggacaccat tgatgttcag ggtcacccac
     1501 agacagggat gcgaggaacc aagcccaatc aagttgtctg tgtggcagca ggcggccagc
     1561 ccgaaggggg tttgcctgtg agccctgaac cttccctgct cactccgact gaggaagcac
     1621 atccagcttc aagcctcgct tcattcccag ctgctcagat tcctattgct gtagaagaac
     1681 ctggatcatc atccagggaa tcagtttcca aggctgggat gccagtttct gcagatgcag
     1741 ccaaagaggt ggtggatgca gggttggtgg gactggagag gcaggtgtca gatcttggaa
     1801 gcaagggaga gcatccagaa ggggaccctg gagaggttcc tgccccatca ccccaggaga
     1861 ggggagagca cttgaacacg gagcaaagcc atgaggtcca accaggagta ccaccccctc
     1921 ctcttcccaa ggagcaaagc catgaggtcc aaccaggagc accaccccct cctcttccca
     1981 aggcaccaag tgaaagtgcc agagggccac cggggccaac ggatggagcc aaggtccatg
     2041 aagattccac aagcccagcc gtggctaaag aaggaagcag atcacctggt gacagccctg
     2101 gaggaaagga ggaagcccca gagccacctg atggtggaga cccagggaac ctgcaaggag
     2161 aggactctca ggctttcagc agcaagcgtg atccagaagt aggcaaagat gagctttcaa
     2221 agccaagcag tgatgcagag agcagagacc atcccagctc acactcagca cagccaccca
     2281 gaaagggggg tgctgggcac acggacgggc cccactctca gacagcagag gctgatgcat
     2341 ctggcctacc acacaagctg ggtgaggagg accccgtcct gccccctgtg ccagatggag
     2401 ctggtgagcc cactgttccc gaaggagcca tctgggaggg gtcaggattg cagcccaaat
     2461 gtcctgacac ccttcagagc agggaaggat tgggaagaat ggagtctttc ctgactttag
     2521 aatcagagaa atcagatttt ccaccaactc ctgttgcaga ggttgcaccc aaagcccagg
     2581 aaggtgagag cacattggaa ataaggaaga tgggcagctg tgatggggag ggcttgctga
     2641 cgtccccaga tcaaccccgc gggccggcgt gtgatgcgtc gagacaggaa tttcatgctg
     2701 gggtgccaca tcccccccag ggggagaact tggcagcaga cctggggctc acggcactca
     2761 tcctggacca agatcagcag ggaatcccat cctgcccagg ggaaggctgg ataagaggag
     2821 ctgcatccga gtggccccta ctatcttctg agaagcatct ccagccatcc caggcacaac
     2881 cagagacatc catctttgac gtgctcaagg agcaggccca gccacctgaa aatgggaaag
     2941 agacttctcc aagccatcca ggttttaagg accagggagc agattcttcc caaatccatg
     3001 tacctgtgga acctcaggaa gataacaact tgcccactca tggaggacag gagcaggctt
     3061 tgggatcaga acttcaaagt cagctcccca aaggcaccct gtctgatact ccaacttcat
     3121 ctcccactga catggtttgg gagagttctc tgacagaaga gtcagaattg tcagcaccaa
     3181 cgagacagaa gttgcctgca ctaggggaga agcggccaga gggagcatgc ggtgatggtc
     3241 agtcctcgag ggtctcgcct ccagcagcag atgtcttaaa agacttttct cttgcaggga
     3301 acttcagcag aaaggaaact tgctgcactg ggcaggggcc aaacaagtct caacaggcat
     3361 tggctgatgc cttggaagaa ggcagccagc atgaagaagc atgtcaaagg catccaggag
     3421 cttctgaagc agctgatggt tgttccccac tctggggctt gagtaagagg gagatggcaa
     3481 gtggaaacac aggggaggcc ccaccttgtc agcctgactc agtagctctc ctggatgcag
     3541 ttccctgcct gccagccctg gcgcccgcca gccccggagt cacacccacc caggatgccc
     3601 cagagacaga ggcatgtgat gaaacccagg aaggcaggca gcaaccagtg ccggccccgc
     3661 agcagaaaat ggagtgctgg gccacttcgg atgcagagtc cccaaagctt cttgcaagtt
     3721 tcccatcagc tggggagcaa ggtggtgaag ccggggctgc tgagactggt ggcagcgctg
     3781 gtgcaggaga cccaggaaag cagcaggctc cggagaaacc tggagaagct actttgagtt
     3841 gtggcctcct tcagactgag cactgcctta cctccgggga ggaagcttct acctctgccc
     3901 tacgtgagtc ctgccaagct gagcacccca tggccagctg ccaggatgcc ttgctgccag
     3961 ccagagagct gggtgggatt cccaggagca ccatggattt ttctacacac caggctgtcc
     4021 cagacccaaa ggagctcctg ctgtctgggc caccagaagt ggctgctcct gacacccctt
     4081 acctgcatgt cgacagtgct gcccagagag gagcagaaga cagtggagtg aaagctgttt
     4141 cctctgcaga ccccagagct cctggcgaaa gcccctgtcc tgtaggggag cccccacttg
     4201 ccttggaaaa tgctgcctcc ttgaagctgt ttgctggctc cctcgccccc ctgttgcaac
     4261 caggagctgc aggtggggaa atccctgcag tgcaagccag cagtggtagt cccaaagcca
     4321 gaaccactga gggaccagtg gactccatgc catgcctgga ccggatgcca cttctggcca
     4381 agggcaagca ggcaacaggg gaagagaaag cagcaacagc tccaggtgca ggtgccaagg
     4441 ccagtgggga gggcatggca ggtgatgcag caggagagac agagggcagc atggagagga
     4501 tgggagagcc ttcccaggac ccaaagcagg gcacatcagg tggtgtggac acaagctctg
     4561 agcaaatcgc caccctcact ggcttcccag acttcaggga gcacatcgcc aagatcttcg
     4621 agaagcctgt gctcggagcc ctggccacac ctggagaaaa ggcaggagct gggaggagtg
     4681 cagtgggtaa agacctcacc aggccattgg gcccagagaa gcttctagat gggcctccag
     4741 gagtggatgt cacccttctc cctgcacctc ctgctcgact ccaggtggag aagaagcaac
     4801 agttggctgg agaggctgag atttcccatc tggctctgca agatccagct tcagacaagc
     4861 ttctgggtcc agcagggctg acctgggagc ggaacttgcc aggtgccggt gtggggaagg
     4921 agatggcagg tgtcccaccc acactgaggg aagacgagag gccagagggg cctggggcag
     4981 cctggccagg cctggaaggc caggcttact cacagctgga gaggagcagg caggaattag
     5041 cttcaggtct tccttcacca gcagctactc aggagctccc tgtggagaga gctgctgcct
     5101 tccaggtggc tccccatagc catggagaag aggccgtggc ccaagacaga attccttctg
     5161 gaaagcagca ccaggaaaca tctgcctgcg acagtccaca tggagaagat ggtcccgggg
     5221 actttgctca cacaggggtt ccaggacatg tgccaaggtc cacgtgtgcc ccttctcctc
     5281 agagggaggt tttgactgtg cctgaggcca acagtgagcc ctggaccctt gacacgcttg
     5341 ggggtgaaag gagacccgga gtcactgctg gcatcttgga aatgcgaaat gccctgggca
     5401 accagagcac ccctgcacca ccaactggag aagtggcaga cactcccctg gagcctggca
     5461 aggtggcagg cgctgctggg gaagcagagg gtgacatcac cctgagcaca gctgagacac
     5521 aggcatgtgc gtccggtgat ctgcctgaag caggtactac gaggacattc tccgttgtgg
     5581 caggtgactt ggtgctgcca ggaagctgtc aggacccagc ctgctctgac aaggctccgg
     5641 ggatggaggg tacagctgcc cttcatgggg acagcccagc caggccccag caggctaagg
     5701 agcagccagg gcctgagcgc cccattccag ctggggatgg gaaggtgtgc gtctcctcac
     5761 ctccagagcc tgacgaaact cacgacccga agctgcaaca tttggctcca gaagagctcc
     5821 acactgacag agagagcccc aggcctggcc catccatgtt accttcggtt cctaagaagg
     5881 atgctccaag agtcatggat aaagtcactt cagatgagac cagaggtgcg gaaggaacag
     5941 aaagttcacc tgtggcagat gatatcatcc agcccgctgc ccccgcagac ctggaaagcc
     6001 caaccttagc tgcctcttcc taccacggtg atgttgttgg ccaggtctct acggatctga
     6061 tagcccagag catctcccca gctgctgccc atgcgggtct tcctccctcg gctgcagaac
     6121 acatagtttc gccatctgcc ccagctggtg acagagtaga agcttccact ccctcctgcc
     6181 cagatccggc caaggacctc agcaggagtt ccgattctga agaggcattt gagaccccgg
     6241 agtcaacgac ccctgtcaaa gctccgccag ctccaccccc accacccccc gaagtcatcc
     6301 cagaacccga ggtcagcaca cagccacccc cggaagaacc aggatgtggt tctgagacag
     6361 tccctgtccc tgatggccca cggagcgact cggtggaagg aagtcccttc cgtcccccgt
     6421 cacactcctt ctctgccgtc ttcgatgaag acaagccgat agccagcagt gggacttaca
     6481 acttggactt tgacaacatt gagcttgtgg atacctttca gaccttggag cctcgtgcct
     6541 cagacgctaa gaatcaggag ggcaaagtga acacacggag gaagtccacg gattccgtcc
     6601 ccatctctaa gtctacactg tcccggtcgc tcagcctgca agccagtgac tttgatggtg
     6661 cttcttcctc aggcaatccc gaggccgtgg cccttgcccc agatgcatat agcacgggtt
     6721 ccagcagtgc ttctagtacc cttaagcgaa ctaaaaaacc gaggccgcct tccttaaaaa
     6781 agaaacagac caccaagaaa cccacagaga cccccccagt gaaggagacg caacaggagc
     6841 cagatgaaga gagccttgtc cccagtgggg agaatctagc atctgagacg aaaacggaat
     6901 ctgccaagac ggaaggtcct agcccagcct tattggagga gacgcccctt gagcccgctg
     6961 tggggcccaa agctgcctgc cctctggact cagagagtgc agaaggggtt gtccccccgg
     7021 cttctggagg tggcagagtg cagaactcac cccctgtcgg gaggaaaacg ctgcctctta
     7081 ccacggcccc ggaggcaggg gaggtaaccc catcggatag cggggggcaa gaggactctc
     7141 cagccaaagg gctctccgta aggctggagt ttgactattc tgaggacaag agtagttggg
     7201 acaaccagca ggaaaacccc cctcctacca aaaagatagg caaaaagcca gttgccaaaa
     7261 tgcccctgag gaggccaaag atgaaaaaga cacccgagaa acttgacaac actcctgcct
     7321 cacctcccag atcccctgct gaacccaatg acatccccat tgctaaaggt acttacacct
     7381 ttgatattga caagtgggat gaccccaatt ttaacccttt ttcttccacc tcaaaaatgc
     7441 aggagtctcc caaactgccc caacaatcat acaactttga cccagacacc tgtgatgagt
     7501 ccgttgaccc ctttaagaca tcctctaaga cccccagctc accttctaaa tccccagcct
     7561 cctttgagat cccagccagt gctatggaag ccaatggagt ggacggggat gggctaaaca
     7621 agcccgccaa gaagaagaag acgcccctaa agactgacac atttagggtg aaaaagtcgc
     7681 caaaacggtc tcctctctct gatccacctt cccaggaccc caccccagct gctacaccag
     7741 aaacaccacc agtgatctct gcggtggtcc acgccacaga tgaggaaaag ctggcggtca
     7801 ccaaccagaa gtggacgtgc atgacagtgg acctagaggc tgacaaacag gactacccgc
     7861 agccctcgga cctgtccacc tttgtaaacg agaccaaatt cagttcaccc actgaggagt
     7921 tggattacag aaactcctat gaaattgaat atatggagaa aattggctcc tccttacctc
     7981 aggacgacga tgccccgaag aagcaggcct tgtaccttat gtttgacact tctcaggaga
     8041 gccctgtcaa gtcatctccc gtccgcatgt cagagtcccc gacgccgtgt tcagggtcaa
     8101 gttttgaaga gactgaagcc cttgtgaaca ctgctgcgaa aaaccagcat cctgtcccac
     8161 gaggactggc ccctaaccaa gagtcacact tgcaggtgcc agagaaatcc tcccagaagg
     8221 agctggaggc catgggcttg ggcacccctt cagaagcgat tgaaattaca gctcccgagg
     8281 gctcctttgc ctctgctgac gccctcctca gcaggctagc tcaccccgtc tctctctgtg
     8341 gtgcacttga ctatctggag cccgacttag cagaaaagaa ccccccacta ttcgctcaga
     8401 aactccagga ggagttagag tttgccatca tgcggataga agccctgaag ctggccaggc
     8461 agattgcttt ggcttcccgc agccaccagg atgccaagag agaggctgct cacccaacag
     8521 acgtctccat ctccaaaaca gccttgtact cccgcatcgg gaccgctgag gtggagaaac
     8581 ctgcaggcct tctgttccag cagcccgacc tggactctgc cctccagatc gccagagcag
     8641 agatcataac caaggagaga gaggtctcag aatggaaaga taaatatgaa gaaagcaggc
     8701 gggaagtgat ggaaatgagg aaaatagtgg ccgagtatga gaagaccatc gctcagatga
     8761 tagaggacga acagagagag aagtcagtct cccaccagac ggtgcagcag ctggttctgg
     8821 agaaggagca agccctggcc gacctgaact ccgtggagaa gtctctggcc gacctcttca
     8881 gaagatatga gaagatgaag gaggtcctag aaggcttccg caagaatgaa gaggtgttga
     8941 agagatgtgc gcaggagtac ctgtcccggg tgaagaagga ggagcagagg taccaggccc
     9001 tgaaggtgca cgcggaggag aaactggaca gggccaatgc tgagattgct caggttcgag
     9061 gcaaggccca gcaggagcaa gccgcccacc aggccagcct gcggaaggag cagctgcgag
     9121 tggacgccct ggaaaggacg ctggagcaga agaataaaga aatagaagaa ctcaccaaga
     9181 tttgtgacga actgattgcc aaaatgggga aaagctaact ctgaaccgaa tgttttggac
     9241 ttaactgttg cgtgcaatat gaccgtcggc acactgctgt tcctccagtt ccatggacag
     9301 gttctgtttt cactttttcg tatgcactac tgtatttcct ttctaaataa aattgatttg
     9361 attgtatgca gtactaagga gactatcaga atttcttgct attggtttgc attttcctag
     9421 tataattcat agcaagttga cctcagagtt cctgtatcag ggagattgtc tgattctcta
     9481 ataaaagaca cattgctgac cttggccttg ccctttgtac acaagttccc agggtgagca
     9541 gcttttggat ttaatatgaa catgtacagc gtgcataggg actcttgcct taaggagtgt
     9601 aaacttgatc tgcatttgct gatttgtttt taaaaaaaca agaaatgcat gtttcaaata
     9661 aaattctcta ttgtaaataa aattttttct ttggatcttg gcaa
//
