LOCUS       NM_003180               3667 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens synaptotagmin 5 (SYT5), transcript variant 1, mRNA.
ACCESSION   NM_003180
VERSION     NM_003180.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Qiu,X., Ge,J., Gao,Y., Teng,M. and Niu,L.
  TITLE     Structural analysis of Ca2+-binding pocket of synaptotagmin 5 C2A
            domain
  JOURNAL   Int J Biol Macromol 95, 946-953 (2017)
   PUBMED   27793683
  REMARK    GeneRIF: Ca(2+)-binding pocket of synaptotagmin 5 C2A domain
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Qiu,X., Huang,K., Liu,Y., Zhang,X. and Gao,Y.
  TITLE     Cloning, expression, purification, crystallization and preliminary
            X-ray diffraction crystallographic study of human synaptotagmin 5
            C2A domain
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun 67 (Pt 11),
            1375-1377 (2011)
   PUBMED   22102235
  REMARK    GeneRIF: crystals of synaptotagmin 5 belonged to the hexagonal
            space group P6(5), with unit-cell parameters a = b = 93.97, c =
            28.05 A.
REFERENCE   3  (bases 1 to 3667)
  AUTHORS   Martins-de-Souza,D., Maccarrone,G., Wobrock,T., Zerr,I.,
            Gormanns,P., Reckow,S., Falkai,P., Schmitt,A. and Turck,C.W.
  TITLE     Proteome analysis of the thalamus and cerebrospinal fluid reveals
            glycolysis dysfunction and potential biomarkers candidates for
            schizophrenia
  JOURNAL   J Psychiatr Res 44 (16), 1176-1189 (2010)
   PUBMED   20471030
  REMARK    GeneRIF: This protein has been found differentially expressed in
            thalami from patients with schizophrenia.
REFERENCE   4  (bases 1 to 3667)
  AUTHORS   Sreenath,A.S., Kumar,K.R., Reddy,G.V., Sreedevi,B., Praveen,D.,
            Monika,S., Sudha,S., Reddy,M.G. and Reddanna,P.
  TITLE     Evidence for the association of synaptotagmin with glutathione
            S-transferases: implications for a novel function in human breast
            cancer
  JOURNAL   Clin Biochem 38 (5), 436-443 (2005)
   PUBMED   15820774
  REMARK    GeneRIF: results suggest a novel putative functional role for the
            GST-synaptotagmin V complex in human breast cancers. As this
            association of GST M1-synaptotagmin was not seen in adjacent
            non-cancerous tissues, this can be used as a marker for breast
            cancers.
REFERENCE   5  (bases 1 to 3667)
  AUTHORS   Mizutani,A., Fukuda,M., Ibata,K., Shiraishi,Y. and Mikoshiba,K.
  TITLE     SYNCRIP, a cytoplasmic counterpart of heterogeneous nuclear
            ribonucleoprotein R, interacts with ubiquitous synaptotagmin
            isoforms
  JOURNAL   J Biol Chem 275 (13), 9823-9831 (2000)
   PUBMED   10734137
REFERENCE   6  (bases 1 to 3667)
  AUTHORS   Craxton,M. and Goedert,M.
  TITLE     Alternative splicing of synaptotagmins involving transmembrane exon
            skipping
  JOURNAL   FEBS Lett 460 (3), 417-422 (1999)
   PUBMED   10556508
REFERENCE   7  (bases 1 to 3667)
  AUTHORS   Fukuda,M., Kanno,E. and Mikoshiba,K.
  TITLE     Conserved N-terminal cysteine motif is essential for homo- and
            heterodimer formation of synaptotagmins III, V, VI, and X
  JOURNAL   J Biol Chem 274 (44), 31421-31427 (1999)
   PUBMED   10531343
REFERENCE   8  (bases 1 to 3667)
  AUTHORS   Craxton,M., Olsen,A. and Goedert,M.
  TITLE     Human synaptotagmin V (SYT5): sequence, genomic structure, and
            chromosomal location
  JOURNAL   Genomics 42 (1), 165-169 (1997)
   PUBMED   9177789
REFERENCE   9  (bases 1 to 3667)
  AUTHORS   Hudson,A.W. and Birnbaum,M.J.
  TITLE     Identification of a nonneuronal isoform of synaptotagmin
  JOURNAL   Proc Natl Acad Sci U S A 92 (13), 5895-5899 (1995)
   PUBMED   7597049
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA529870.1, BC046157.1, BQ417474.1 and AC010327.8.
            
            On Nov 23, 2018 this sequence version replaced NM_003180.2.
            
            Summary: Synaptotagmins, such as SYT5, are a family of type III
            membrane proteins characterized by cytoplasmic repeats related to
            protein kinase C (see MIM 176960) regulatory (C2) domains, which
            are thought to bind calcium. Synaptotagmins may act both as
            negative regulators of vesicle fusion, allowing fusion in the
            presence of calcium, and as calcium receptors or sensor molecules
            (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied
            by OMIM, Feb 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1100744.1, BC046157.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354308.8/ ENSP00000346265.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                DA529870.1         64-74
            12-547              BC046157.1         1-536
            548-553             BQ417474.1         524-529
            554-1762            BC046157.1         543-1751
            1763-3667           AC010327.8         64642-66546         c
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.42"
     gene            1..3667
                     /gene="SYT5"
                     /note="synaptotagmin 5"
                     /db_xref="GeneID:6861"
                     /db_xref="HGNC:HGNC:11513"
                     /db_xref="MIM:600782"
     exon            1..173
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            174..297
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..1379
                     /gene="SYT5"
                     /note="isoform 1 is encoded by transcript variant 1; sytV;
                     synaptotagmin V"
                     /codon_start=1
                     /product="synaptotagmin-5 isoform 1"
                     /protein_id="NP_003171.2"
                     /db_xref="CCDS:CCDS12919.1"
                     /db_xref="GeneID:6861"
                     /db_xref="HGNC:HGNC:11513"
                     /db_xref="MIM:600782"
                     /translation="MFPEPPTPGPPSPDTPPDSSRISHGPVPPWALATIVLVSGLLIF
                     SCCFCLYRKSCRRRTGKKSQAQAQVHLQEVKGLGQSYIDKVQPEVEELEPAPSGPGQQ
                     VADKHELGRLQYSLDYDFQSGQLLVGILQAMGLAALDLGGSSDPYVRVYLLPDKRRRY
                     ETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEVRVPMSSVD
                     LGRPVQAWRELQAAPREEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSD
                     PYVKVHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKL
                     GKNEAIGRVAVGAAAGGAGLRHWADMLANPRRPIAQWHSLRPPDRVRLLPAP"
     exon            298..470
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            471..590
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            591..758
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            759..926
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1044
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1178
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..3667
                     /gene="SYT5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1744..1749
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT5"
                     /note="hexamer: AATAAA"
     polyA_site      1767
                     /gene="SYT5"
                     /note="major polyA site"
     regulatory      3640..3645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT5"
                     /note="hexamer: AATAAA"
     polyA_site      3667
                     /gene="SYT5"
                     /note="major polyA site"
ORIGIN      
        1 acacgggcac acacgcgcgc acgggatcct gctccgctcc gcggctcccc cgccccggag
       61 agctccgcga tcagcacccg ggacagcgcc accgcccacg tgcagggggt ggggtccggg
      121 cggggctggc gcctcggcgt ctcccgggag tgtcccgtcc agccgccgag cagggtgctt
      181 gagtgtctct gcagaaaaga ctccaggacc ccgccaccat gttcccggag cccccaaccc
      241 cggggcctcc atcgcccgac acgcctcccg actccagtcg catcagccac ggcccagtgc
      301 ccccctgggc cctggccacc atcgtgctgg tctcaggcct cctcatcttc agctgctgtt
      361 tctgtctcta ccggaagagc tgtcggaggc ggacaggcaa gaagagccag gcccaagccc
      421 aggtccacct tcaggaagtg aaggggctgg gccagagtta catagacaag gtgcagccag
      481 aagtagagga gctggagcca gcaccatccg ggccagggca gcaggtggca gacaagcatg
      541 agctaggacg actgcagtac tccctggatt atgacttcca gagtggccag ctgctggtgg
      601 gcattctgca agcaatggga ttggcagcct tggatcttgg tggctcctcg gacccctatg
      661 tgcgggtcta cctgctgccg gacaaacgga ggcggtacga gaccaaggtg catcggcaga
      721 cgctgaaccc tcactttggg gagaccttcg ccttcaaggt cccctacgtg gagctggggg
      781 gcagggtgct ggtcatggcg gtgtacgact tcgaccgctt ctctcgcaat gacgccatcg
      841 gggaggtgcg ggtccctatg agctccgtgg acctggggcg gccagtgcag gcctggcggg
      901 agctgcaggc ggctccgcgg gaggagcagg agaagcttgg ggacatctgc ttctccctcc
      961 gctatgtccc cacggccggg aagctcaccg tcatcgtcct ggaggctaaa aacctgaaga
     1021 agatggacgt aggaggactg tcagatccat acgtcaaggt ccacctgctg cagggcggca
     1081 aaaaggtgcg gaagaagaaa accaccatca agaagaacac tctgaacccc tattacaacg
     1141 aagctttcag cttcgaggtg ccctgtgacc aagtccagaa ggtgcaggtg gagctgaccg
     1201 tgctggacta cgacaagctg ggcaagaacg aggccatcgg gagggtggcc gtgggggcgg
     1261 ccgccggcgg ggctggcctg cggcactggg cggacatgct ggccaacccg cggcggccca
     1321 ttgcccagtg gcactcgctg cggcccccgg accgagtgag gctgctgcct gcgccctgac
     1381 tcccacccca agcccccggc caagcctgga ctctagcccc tgaccccaga ctcccgagac
     1441 cggccaaagc caagccagcc ccacccttaa gcttcctctg acggttaccc tccccccacc
     1501 ttccacccca accatctgaa ccattcctgt cttctcaccc ccaacgatgc caatcacgac
     1561 aactttccag cacactcccc cgaccctcgc ggacacaccc agatgcccca gggaccccgg
     1621 gaagggaagg cagcctggtt tctcctgccc ccgggtcctg ccccctgctt tgacaatcag
     1681 ccatcctcgt ctgctctcct ctctcttcca acacagccca tccctgcact gctcctgggg
     1741 ccaaataaat attcagcagc tccggtggcc tgggcccatg ctccttgatg ggggtgggca
     1801 agggctggga cccaagagga aacctcgttt ccaggaatgg gcgctcgcag ggcggtgtga
     1861 gtgctgttcg tcctgaaagc ctctgagaga ctggcctgac cccatggact ccctcacact
     1921 ctcacccgtt cattcctcac tcaccactct gagcctgggt ttcctcctct ggagaatgtt
     1981 gggataataa taattcctgc cacacgctgg ctgtgaggag tccgcgagaa ctcccttgct
     2041 tgtccatgaa tttatccact cgcttgctca cccatgactc acatcgattt tcatacccat
     2101 ttctgcatcc ctccctccct ctcttttttt tcttccttcc attccatatt ccctgaggtc
     2161 ttctgttgag tgctggggcc ccagaatatc caaacacaga cctgaccccc caagcgactt
     2221 actgccatca aaacaaagga gaggcgcgga gccagggaat acaatctcca gagcctgcga
     2281 gagaggttgg agttcagcaa ggaaagccaa tgatttcaac ttcacctctt tcttttttct
     2341 tttctttttt cctttttgtt ttctttctgt ctttcctttt ctttcttttt ctcttgtttt
     2401 ttttttgttt gtttgttttt ttgtttttgt ttttgttttt ttgagatgaa gtctcacttt
     2461 gtcgcccagg ctggagtgca gtggcacgat ttcggctcac tgcaagctcc tcctcccggg
     2521 ttcacgccat tctcctgcct cagcctcccg agcagctggg actacaggcg cccgccacca
     2581 cacccggcta atttttttgt atttttagta gagacggcat ttcaccgtgt tagccaggat
     2641 gatctcgatc tcctgacctc gtgatccgcc cgcctcggcc tcccaatgtg ctaggattac
     2701 aggcgtgagc caccgcgccc ggcccttctt cttttctttt cttttctatt tcttttcttc
     2761 tcttcttctt ttttttaatt ttgagacgga gtttcgctgt tgttgcccag aatggagggc
     2821 aatggtgcaa tctcggctca ccgcaacccc tgcctcccgg gttcaagcga ttcttctgcc
     2881 tcagcctctt ctttcttctt tttttgacag ggtttctctg tgtaccctgg ctggagtgcg
     2941 gtggggcaat catagctcac tgcagcctca acctcctggg ctcaggggat cctcctgcct
     3001 cagactcctg agtagctggg accacaggca catgcaacca tgcctagcta attttttctt
     3061 ttcttttctt ttctttttta gagatggggt cttgctatgt tgcacaggct ggtttcaaac
     3121 tcctgcaatc ctcctgtgtg agtagctggg actgtagaca tgagccgccg catctgacct
     3181 cattgttctt tcaacgaatg ctgcctccta ctctgtttct agcaaagatg atagttccat
     3241 tggcttctac cttatctcat cccatacaga attttgggtg gtgaacaaag acctataaga
     3301 tttggccaga gaactctctg agcagcagat taagtggaga atgaggcaaa aattaccccc
     3361 acgtaccaaa aaaggaggca gcaaacaaac ttacttgctc ctaagcttcc tggtagccaa
     3421 tcgggaaagt gaaaatagaa cagctacaca gattataata tccatgagat ttttaatttt
     3481 tctccaaagg aattcagtaa cttttcatgt gtgtgagtgt gtgtgtgtgt gtgtgtgtgt
     3541 gtgtgtgtgt gtgtgtggct gtagagcatg tctttatgaa gacagccctt tatgttacta
     3601 ctattactgc caataataat cctcacagca aactcatgaa ataaacatga tcattattcc
     3661 cagttga
//
