LOCUS       NM_001146262           12046 bp    mRNA    linear   PRI 12-JUN-2024
DEFINITION  Homo sapiens synaptotagmin 14 (SYT14), transcript variant 3, mRNA.
ACCESSION   NM_001146262
VERSION     NM_001146262.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   2  (bases 1 to 12046)
  AUTHORS   Sheng,B., Jiang,Y., Wu,D., Lai,N., Ye,Z., Zhang,B., Fang,X. and
            Xu,S.
  TITLE     RNAi-mediated SYT14 knockdown inhibits the growth of human glioma
            cell line U87MG
  JOURNAL   Brain Res Bull 140, 60-64 (2018)
   PUBMED   29634997
  REMARK    GeneRIF: SYT14 mRNA expression was detected in the three glioma
            cell lines, and was highest in the U87MG cell line. The
            RNAi-mediated knockdown of SYT14 significantly decreased cell
            proliferation and colony formation in U87MG cells, and caused a
            moderate increase in apoptosis. Fewer S phase cells and more G2/M
            phase cells were observed.
REFERENCE   3  (bases 1 to 12046)
  AUTHORS   Requena,T., Espinosa-Sanchez,J.M. and Lopez-Escamez,J.A.
  TITLE     Genetics of dizziness: cerebellar and vestibular disorders
  JOURNAL   Curr Opin Neurol 27 (1), 98-104 (2014)
   PUBMED   24275721
  REMARK    GeneRIF: Whole-exome and targeted sequencing have defined the
            genetic basis of dizziness including new genes causing ataxia:
            GBA2, TGM6, ANO10 and SYT14
            Review article
REFERENCE   4  (bases 1 to 12046)
  AUTHORS   Sailer,A. and Houlden,H.
  TITLE     Recent advances in the genetics of cerebellar ataxias
  JOURNAL   Curr Neurol Neurosci Rep 12 (3), 227-236 (2012)
   PUBMED   22527681
  REMARK    GeneRIF: New DNA sequencing technologies are enabling us to
            investigate the whole or large targeted proportions of the genome
            in a rapid, affordable, and comprehensive way. Exome and targeted
            sequencing SYT14 genes causing ataxia.
            Review article
REFERENCE   5  (bases 1 to 12046)
  AUTHORS   Doi,H., Yoshida,K., Yasuda,T., Fukuda,M., Fukuda,Y., Morita,H.,
            Ikeda,S., Kato,R., Tsurusaki,Y., Miyake,N., Saitsu,H., Sakai,H.,
            Miyatake,S., Shiina,M., Nukina,N., Koyano,S., Tsuji,S., Kuroiwa,Y.
            and Matsumoto,N.
  TITLE     Exome sequencing reveals a homozygous SYT14 mutation in
            adult-onset, autosomal-recessive spinocerebellar ataxia with
            psychomotor retardation
  JOURNAL   Am J Hum Genet 89 (2), 320-327 (2011)
   PUBMED   21835308
  REMARK    GeneRIF: A homozygous missense mutation in SYT14, encoding
            synaptotagmin XIV was identified in a Japanese family in which two
            siblings have slow progression of a type of autosomal-recessive
            cerebellar ataxias.
REFERENCE   6  (bases 1 to 12046)
  AUTHORS   Quintero-Rivera,F., Chan,A., Donovan,D.J., Gusella,J.F. and
            Ligon,A.H.
  TITLE     Disruption of a synaptotagmin (SYT14) associated with
            neurodevelopmental abnormalities
  JOURNAL   Am J Med Genet A 143A (6), 558-563 (2007)
   PUBMED   17304550
  REMARK    GeneRIF: Constitutional rearrangement of SYT14 may contribute to
            macrocephaly, cerebral atrophy, seizures and developmental delay.
REFERENCE   7  (bases 1 to 12046)
  AUTHORS   Craxton,M.
  TITLE     Synaptotagmin gene content of the sequenced genomes
  JOURNAL   BMC Genomics 5 (1), 43 (2004)
   PUBMED   15238157
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 12046)
  AUTHORS   Fukuda,M.
  TITLE     Molecular cloning, expression, and characterization of a novel
            class of synaptotagmin (Syt XIV) conserved from Drosophila to
            humans
  JOURNAL   J Biochem 133 (5), 641-649 (2003)
   PUBMED   12801916
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL022399.2, AL022397.1,
            AL513263.9 and KF455151.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 18, 2021 this sequence version replaced NM_001146262.3.
            
            Summary: This gene is a member of the synaptotagmin gene family and
            encodes a protein similar to other family members that mediate
            membrane trafficking in synaptic transmission. The encoded protein
            is a calcium-independent synaptotagmin. Mutations in this gene are
            a cause of autosomal recessive spinocerebellar ataxia-11 (SCAR11),
            and a t(1;3) translocation of this gene has been associated with
            neurodevelopmental abnormalities. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene, and a pseudogene of this gene is located on the long arm of
            chromosome 4. [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (3) lacks an exon in the 5' coding
            region and initiates translation at an alternate start codon,
            compared to variant 1. The encoded isoform (3) is shorter and has a
            distinct N-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC144155.1, AJ617623.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367019.6/ ENSP00000355986.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                AL022399.2         35175-35235
            62-109              AL022399.2         49667-49714
            110-274             AL022397.1         82913-83077         c
            275-490             AL022397.1         75456-75671         c
            491-762             AL022397.1         2117-2388           c
            763-1212            AL513263.9         5307-5756
            1213-1402           AL513263.9         61016-61205
            1403-1459           AL513263.9         64716-64772
            1460-7033           AL513263.9         66024-71597
            7034-7363           KF455151.1         1-330
            7364-12046          AL513263.9         71928-76610
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.2"
     gene            1..12046
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="synaptotagmin 14"
                     /db_xref="GeneID:255928"
                     /db_xref="HGNC:HGNC:23143"
                     /db_xref="MIM:610949"
     exon            1..61
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1773
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="isoform 3 is encoded by transcript variant 3;
                     synaptotagmin XIV"
                     /codon_start=1
                     /product="synaptotagmin-14 isoform 3"
                     /protein_id="NP_001139734.1"
                     /db_xref="CCDS:CCDS53470.1"
                     /db_xref="GeneID:255928"
                     /db_xref="HGNC:HGNC:23143"
                     /db_xref="MIM:610949"
                     /translation="MAIEGGERTCGVHELICIRKVSPEAVGFLSAVGVFIILMLLLFL
                     YINKKFCFENVGGFPDLGSEYSTRKNSQDKIYNSYMDKDEHGSSSESEDEALGKYHEA
                     LSRTHNSRLPLADSRQRNYAWETRQKYSPLSAEYDGYSSEASIDEGNCIQRMRRTPPL
                     DELQPPPYQDDSGSPHLSCTPSEIGDSKCEFSHCSNSPRCSYNKCPSEGSTGHEIESF
                     HNKGYEEDVPSDSTAVLSPEDMSAQGSSSQLPKPFDPEPEAKYGTLDVTFDYDSQEQK
                     LLVTVTAVTDIPTYNRTGGNSWQVHLVLLPIKKQRAKTSIQRGPCPVFTETFKFNHVE
                     SEMIGNYAVRFRLYGVHRMKKEKIVGEKIFYLTKLNLQGKMSLPVILEPSYNHSGCDS
                     QMSVSEMSCSESTSSCQSLEHGSVPEILIGLLYNATTGRLSAEVIKGSHFKNLAANRP
                     PNGLFCCLKHLIGGQVYIIRDTYVKLTLLNSMGQEMSKCKTSIRRGQPNPVYKETFVF
                     QVALFQLSDVTLILSVYNKRSMKRKEMIGWISLGLNSSGEEELNHWTEMKESKGQQVC
                     RWHALLES"
     exon            62..109
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            110..274
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            275..490
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            491..762
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            763..1212
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1402
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1459
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..12046
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11273..11278
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="hexamer: AATAAA"
     polyA_site      11299
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="major polyA site"
     regulatory      11452..11457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="hexamer: AATAAT"
     regulatory      11462..11467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="hexamer: ATTACA"
     polyA_site      11475
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="major polyA site"
     polyA_site      11482
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
     regulatory      11542..11547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="hexamer: TATAAA"
     polyA_site      11562
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
     regulatory      12024..12029
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="hexamer: ATTAAA"
     polyA_site      12046
                     /gene="SYT14"
                     /gene_synonym="SCAR11; sytXIV"
                     /note="major polyA site"
ORIGIN      
        1 gtctgctgcc cccgccatcc agttggtgcg gtccatggcg agcgcatcat ggcgattgaa
       61 ggtggagaga gaacctgtgg agtacatgaa cttatctgta ttagaaaagt atctccagag
      121 gcagttggat ttttgtcagc tgttggggtg tttattatct tgatgctgct cctttttctc
      181 tatattaata agaagttctg ttttgaaaat gttggcgggt ttccagatct tggttcagaa
      241 tacagtacaa ggaagaattc acaagataaa atttataatt cctacatgga caaagatgag
      301 catggttcat cctctgaaag tgaagatgaa gcgctgggta aatatcatga ggccttatcc
      361 agaacacaca attccagact accactggca gattctagac aaaggaacta tgcttgggaa
      421 acaaggcaga aatacagtcc tctatcggca gagtatgatg gatacagtag tgaagcatca
      481 atagatgaag gaaactgcat tcagagaatg agaagaacac ccccgctgga tgaattgcag
      541 ccaccaccat atcaggatga cagtggttct ccccatctgt catgtacacc ctcagaaatt
      601 ggggacagta aatgtgaatt ttcccactgc agcaacagtc caagatgctc atataacaag
      661 tgcccaagtg aaggaagcac aggtcatgaa atagaaagtt ttcataataa aggatatgaa
      721 gaagatgttc caagtgacag cactgcagtc ctgagccctg aagatatgtc agctcaagga
      781 tcatcttcgc agcttcctaa accttttgat cctgagccag aagctaaata tggcacactg
      841 gatgtgactt ttgactatga ctcacaagaa cagaagcttc tggtaacagt gacagctgtc
      901 acagacatcc caacatataa caggacaggt ggcaactcat ggcaagtaca ccttgttctt
      961 ctacctataa agaaacagag agcaaaaacc agcatccaga gaggaccatg ccctgtcttc
     1021 acagaaacat ttaaatttaa tcatgttgaa tctgagatga ttggaaatta tgcagttcgg
     1081 tttagactgt atggtgtaca tcgcatgaaa aaagaaaaga ttgtggggga aaagattttt
     1141 tatttaacaa aattgaatct tcaagggaaa atgtcattgc ctgtgatatt ggaaccttct
     1201 tacaatcatt ctggctgtga ctcccaaatg agcgtgtcag aaatgtcgtg tagtgaaagt
     1261 acatcctcat gtcagtctct tgaacatggc tcagttccag aaattcttat tggcctgctt
     1321 tataatgcca caactggaag actatcagca gaagtgataa aaggcagcca cttcaaaaat
     1381 ttggcagcaa acagaccacc caatggactg ttctgttgtc taaaacactt gataggtgga
     1441 caggtttata taatccgaga tacatatgtt aagttaactc tactgaattc catgggtcaa
     1501 gagatgtcca aatgcaagac atccatccgc agagggcagc caaatccagt atataaggaa
     1561 acttttgtct ttcaagtggc cctatttcag ctttctgatg tgacactcat actgtctgtg
     1621 tataacaaac gcagcatgaa aagaaaagag atgataggct ggatttcttt aggtctcaac
     1681 agctctggag aagaagaact caatcactgg actgaaatga aagagtcaaa aggacagcaa
     1741 gtatgtagat ggcatgcgtt gctagagtca tgatgaatag aataagcaag cagttaccat
     1801 caaaggcagc atatttccaa ttccaacatt actgtttcta ccaagtccca ttagaagagc
     1861 tgttctttga agaatcatat tcaaccttct accaaaatgc tttaagttct atggaaagaa
     1921 cgtctcatac tgacataaat gaagaaaata tgtgtatcta gtagagcttg tttaggaaac
     1981 tgagaaacgt acactatcat tgctaaacta acagtcctcc agaaatttaa taagatgttt
     2041 ttgatttgaa gttaatttta atttagcaaa agagccagtc attttatgaa aaatcaaaat
     2101 tataagtgat tttaaaaacc agaattttag ttgcaataca attttaatat caccctacat
     2161 attatttata aaacatagtt tgactggcca gtccctggtg tttgtaatgt tctttaatat
     2221 gaaaaagtag ttctccaact ctatcataag tcatatctaa tggtgaaggg tttcagtcac
     2281 attgaaaatt gttttatttc aggcatgttc cctttgtgca cttaggttca ttgtgcctca
     2341 gttcctctca aaagcactaa atctaagtgc aaacaagtat tcattttcat acaggaattt
     2401 ttttaatatg ttattttttt aaaagttcat ttttcatttg cctccgcttt cgcctgatcc
     2461 aaagagacca agtcactgta ctggtccctt gcaagtcttc tagacaggtt gtactgtaga
     2521 actacgtaac tttctgttga aagcacttcc tgtattcttg tattccaatg taggaagcta
     2581 atagagcagg actttacttt caaatttctt tcagtgattg actcttcaaa attgcagtag
     2641 tgtgaaaata cattttttac aaactgaaaa tacagtagat aagctgtcga aaatggaaaa
     2701 caattcaatt ttaattttct gctctgagta tttagtaacc aacactgcgt aaagcaggta
     2761 gcattcaaaa taagaaactg ttctctctct aaatcttcac tcttacttca atttatattt
     2821 gtgatatgaa gactatccag ttctcagttt gaattggaac atcatgttaa aaacagttat
     2881 gagattcctt atgaagtttc tagtaacttg taactagact ttatgaaatt aacagataaa
     2941 gttcttcaac gtgatgtcat cttgtgcctt tcatgtaagt taaatttgct catacagctt
     3001 gaacgttgta tttgcaaaat taagccttcg atttttataa atgtaaagat tcctcagaac
     3061 agtgtcgcaa gatctttatt tcctctgaag tatgtaaaag ttgtataatg tgctaacttt
     3121 ttaatggcaa ggcactttca tttgttttta tatttaggaa ctctaatcag gaatattata
     3181 agctgttttc tttttaattt tgcttcttac ctaatatatc attttatgca tatactttat
     3241 cagagtatgt tttatatata ttaaaaatag tttctttttt ctccttctca tttctagtta
     3301 aaatagacaa aaacatacta tttaccagtt actaaaaagc agtatatcat tgcttctagt
     3361 cccaactaag tactatatat tattgtaaac tagaacttgt tattaccata ataacaaaac
     3421 aatgcgtaga tattacttca gcttggctgt actgaaaggc cttaaaagaa acccaaaaag
     3481 accgtgaact tctgtatttt tttagtttaa aaaatggcag ctctatgcat tcatacacat
     3541 gaattataag aaaaaataca aatgctatat tggatgacat gaggtttgag aatgtgtgtt
     3601 acagggctgc attaaaggga aaaatccatc ttttattgtt acatcaatga gctttttcca
     3661 ggatttttca aataaacaag taagagttag aacctgaatc aaatggcaaa atatctcatt
     3721 taagtgtttt cagcaacagt atgaaaaaag acttgaagag gggatgtaga ttttactgag
     3781 aaattggact taggcatttg aagctataat gaacttttta gactttgaaa caactgactg
     3841 cattgctaac aactgttatc ttcctaataa aaagagttat cagtcttatc tgtaactgta
     3901 acagagctta gtgaaacaca ttagtaagac cagcttggtg ctttcactct tagtgagaaa
     3961 aagacctaac taattgttat ttgttaaatc tacctttttt cagactattc ccagaattgt
     4021 gtgtgtgatt tgatacatcc ctagggattt atctggcata agtatcaaat ccttcatggc
     4081 ttaaaaatta ataaacagtt ttttaatatt tctggaaaga ccctacctcc accccagctg
     4141 ccaagtcctc aaagattata aattatctat atgagtgtat gtatctgtat acacacatgc
     4201 accattacac atacaacata aatatgtata tgtatattca tttgtaaagg caaaggtgcc
     4261 ctggcttttt cagataactg aatagaaaaa catttcatac atttaggatt atttttctcc
     4321 tctatgtatt tactagatat tatatttggt ttgggttgct ttctgctgct gttctccttc
     4381 acacacagca aaatatacat ttgtttctgt agattgacac aacttataat aattactccc
     4441 aaaaagttgt ctgttctttt agaaaaggat taaataaggc tcaggggacc cacttcactc
     4501 tactgaatct aatgtacgag acagtactac tgcttctttg tgtgcttgaa tttgcatgaa
     4561 agcttaatag caaaatcttc tatctcagta attatagcaa atagggacct actggtaggg
     4621 agcaggtgtt ggctcagaaa cctgaaaaag acaacccact ttcatacagt tctattaaaa
     4681 tcccctaagt tttctaataa aatgatatag tccaaatatg gcaatcgact ggcatttatg
     4741 ggagcctcta aacctggaat tatggggttt atttttctcc caaatgagct gcaaaagata
     4801 attattgctg cccagtaaac aaaatgtatg tcaaatcagt aaagaatttg tttagcataa
     4861 tattgcctta tctttcagaa gctttcttct atattcttgt gtcttcagta caatccactt
     4921 ttgtttgaaa tccatttgca tattatctta ctgtgcattt agtgaattct caataatgtt
     4981 gtatttgctg acaagaataa taaattggac cacaattaaa aaaaaagaaa acccaatgag
     5041 aaaataacta cctgattaat atacacatgt ttaaatgtag gacctggtaa agcagtttca
     5101 tccaacctag cgaaaatgcc cagggctgtt tgttaaatca tttgcttgat accatcatca
     5161 ttttcatttc acttatcaag tgaatcagaa gcataatcaa taactgcaaa gtaagatagt
     5221 accaatagga gaaagaggta aaagaattga gagtgtttgt caatatgaag atgtgtgggg
     5281 atactgaatg atatcatagt ctttccagtg aaagaaaaaa taggctatgg gaagatgtaa
     5341 atttttgatg gagtaagaac agtggtcacc accaagtatt tttcaagatc cctctccagc
     5401 tatgaataga aaggacacaa attcatttgc gtaaaattgc catttgccta catagactag
     5461 acaattcaca gatttttcac aaagacagtt attatatata tagttatttg agttatcact
     5521 atattatttc acattgatag tttttatttc taattttgtt tccattcact gtcctcagtt
     5581 cctctgtggc ctacattgaa ggtagctgtc agtattatta ttgtcaacca tatttaatat
     5641 tgaatacaag tttaagcatg ctgcaaagca ttttctaacg ttaattttaa aacattgtct
     5701 ctgccctcca aagtgatggt taacagctat aatttactaa gatctagcac aataatttat
     5761 tacttgcttg attctcaaaa caatactgtg aggtaagtaa tgttatcaac attttattga
     5821 taaagaaact gcagcatgaa gaggttaagc agactttccc aaggtcattc ggtaagtaag
     5881 tagtagaact gggattggaa cccaggtgta aattactcta aaacccatgc tatgcatgtt
     5941 gcatttgcct ccctgtttga aaaaaaaact acttaggaca ttatatttat caataatccc
     6001 actcaccttg attgaatggc agaactaagg gggaaaaaac agctggggaa agagaaaaga
     6061 ctagaaaaga agatcagaaa ttttttttct tctcaggaca caagtggtgg tacttttgtg
     6121 tcattttgta gatgactttg atttgcaaag atttggaatt gatttattct accagtttag
     6181 cctatactta tattccaaaa gcatattaat cagttctgct ccttgaccac ttttaggaat
     6241 ccaaggtgtt ttaattgtta catgaccccc ttactactct atgaagggca taacctgtgt
     6301 ctagcccata tctcactcat atcagtggtg agacaaaaga tttgtcttcc tctgtcctct
     6361 cattgcaatt tatttatgtt ttattctcaa ttttattaag atttgtcagg aattacagct
     6421 agtttattac atatgtctct cttcccccta ggtaagtagt caatgccttg ttagtatctt
     6481 tctgtacccc tttgcttata gtaggcagac agtaaatatt gaattgaact aaattccctg
     6541 tggaagaagc cagtcacact attacatgag tcttagtttt tatgaatctt gtgttatgta
     6601 tggatttatt tattccttca gttaatatat atttaagagt actagatatg tgccatgtgt
     6661 tgttgttcca agtgctacag atgcagagat aataaattgg tcgaagtctt tgccctcatg
     6721 gggcctacat tctaaggtag gaagatggcc acagaatggg ataatttcag gctagtataa
     6781 tgaagcatta agataggggc atgcatagag taccgtggga tttagtagaa gagtgcaaca
     6841 tggaattcaa aaaaatcttc ctggaaaaga taatccctga gttgggtctt aaagaacaaa
     6901 tacatgttag actcccatca aacttgagga cacacgttct ttccaagaac aaaatgtaaa
     6961 ataatggttt ctaaactgta gtatccttaa gaattatttt gaaaatttaa cttgggccag
     7021 gcgtggtggc ttacgcctgt aatcccaaca ctttgggagg ctgaggtagg cagattactt
     7081 gagctcagga gtttaagacc agcctgggca acatagcaaa accccatctc taccaaaaat
     7141 ttaaaaatta gctgagcacg gtggtgcatg cctgtggtcc cagctactca ggaggcttag
     7201 gtgggagaat cgctggagcc tggcaagtca aggctgcagt gagccaagat agcgccactg
     7261 cacttcagcc tgggtgacac agaagacctt gtagaaaggg aggaagaaag gagggagaga
     7321 gagaggaagg gaggaggaga agggaaggaa ggaggagacg ggaaagaatg aaggagaagg
     7381 gaaagaaggg agggaaattt aacttgtatt ccaaaactcc acctccagag attctacttc
     7441 atttggcctg gaagggagcc caataatcta cattttatta agctctccag gaaattctgt
     7501 ctgagatcta ttgatataga atattaagtg tattacatag tagtattcat gaaatgtttc
     7561 agtgcctgta gatcagacct ttaagtgtga tagtgatagt aatacagaga ttaagactag
     7621 gattaatgtt attcatcttt ttcttgtgct tccttaaaaa ggaagatatt taatgaaaaa
     7681 aattaatatt taataatgaa tttctgtaca ttcacattca acatacattt ttcaaagtgt
     7741 ttcttggtat aataaatatg ttgattttat ggcttccaaa aattcctttc tatcactgtt
     7801 caaatatttc cttctaagtt atctttacaa tgatggatat aaagtgaaga atggggaggt
     7861 atttatcaag taatttcttt atcagtatcc taaatgaaat aagactgacc tttgagcaaa
     7921 tgtgtagtag cagccatagg tgctggcatg tttgcattct tgcatacagt aagatttctt
     7981 gcttcccttt atcatcatct aaatggcttc attgtcatca cactcatcag atgaaatgca
     8041 gtactggatt cttctctaaa agcagtgatt ctgtgactgc tgctctaaac tctagttcct
     8101 tgattgtttt gttcttagga tcctttgttt caaatagcaa gttaatacag cagcctcagg
     8161 accttctctt tctctcagaa taaatgctgt ggataaggta catctgagtt tccatttccc
     8221 ctcatttcac atatagccat attttcttct ctgatgtaat attttgtatt aacacatttt
     8281 acattttgaa gttttctttt cccccacaaa gatatgtggt tatcccactt aaaaatgagc
     8341 ttatttgaat atgtgaaata atgttgtcat tgaattaagc tgttttgtga ctgctgatgt
     8401 atcactgtgt ttgatttggt gacagcttga ggtctggtta atataatctt aaggtaatca
     8461 gcttgataga ctggaattac tagtttatta tcaaaaaatg aatgtaaaaa tattgctctt
     8521 ttctctccct gtttagacta gtatgtgcct ttttaaaaat actccctttc ttcttccctg
     8581 ccttttttcc ccactcttat tcatatacac tcaaactaga taccagagtt agttcatcaa
     8641 agctgaataa tacctatcaa aaagtgatac cctgactttt ttctcttaat cacctgtact
     8701 ggaccatagg gaggaccaaa taggctttct tggggactac agaaagagca aagcgtaata
     8761 ctctttaatc atagcatgtt tgttgttgct gtttcggtgg tggtggtggt ggtggtggtg
     8821 gttgtgtact taccttgaat agtgtgaagt atcctaaaat tatgttgtgt gggctgggag
     8881 caggaaggaa actgattatt cgtctaacaa aggtttggca taattttatt gtaaaatttc
     8941 aactgtttat gacatgaaaa tttctaatta attataagaa caaagtcact gtggtggcaa
     9001 tagttgtaca agatgtttga cattatttct atgtcagttt gatagtctgt gttataaatt
     9061 gtcttattag gacccttctt caccctcttc tttattattc tgattatcag taaatccagt
     9121 ttgtactgtt agacatttga gtttgtcttt taaatattga agactatttt aaaaagaaaa
     9181 accataacat ctttctgttt attacattaa catgtgatct ctcacctgac gatgtgttta
     9241 aaatgaatta atagataaaa tataatactc cgttatatac tgaaaaaaat tttcaaactt
     9301 tgtctatata ttcagatatt gctagcagtt catattaaat atgactattt tgtttaatga
     9361 gaaaatagga atttttcctt atctctgaga cttactgctt ctctttgcca ttcacatctg
     9421 gttgaaggtg gatggttttg aacctcgtaa cgtcacttca ctcgtcagtt tctgagtgtg
     9481 ccaacctcct tctgacggtt taattttcag taacaatcaa accatttatt cctcaggggt
     9541 tggtcctcct agtgattcct gtccttaacc cccattagtg ttgatggaag taatccataa
     9601 gcaccaatag gagaaaaggc ccataaagga tcagtatccc agaaccctga aataggacgg
     9661 ccgcttcagt acccaaatgt cattgcaatt ctaatggagt agaagccagc ttcttggaag
     9721 tgaagatttg agatcattat gtcatattct ctccttttgg tgtcctccct tctccctcta
     9781 gcttgcacat tctttttatt ttaaatttta caggaagcct tcttttaaaa acctggtttt
     9841 taaaatttca catgttgctg taaccaggtg atttcaaaac ccagaaaact gaagtacata
     9901 ccatacagtc ataagaagtt tgggggcttt tttggttttt atgtttttgg tgtttttttt
     9961 tttttttttt tttttttttt ttgtagtgtg gggagtgggg aggaggaaaa gggagaaatc
    10021 gtgcttgttt tggttttggg aagtcttttt ggataaatat tgagaatatt tatagtattc
    10081 tgtaattatg tgacatttgt tgtgtgaggg gagaccagag tagctgttgc acatacatcg
    10141 actctcttaa tattgaagca tatttaaaca gttgtttgaa gcagagctag aaattaatgg
    10201 tgatacttct aacagcattt tgatgttata aatattactt taagtttatc aagagaaaaa
    10261 ttccagtagt agcttgcgtt ataaaattaa ttgatttatt atgtttctgt ataattttat
    10321 tgaaattgct tttttgtggt gaatataata tagtggcaac gattttgagg aatctttaaa
    10381 gctgtttgga agagatggcc agcctttggc tagattttta ctagaatttt gaaataagaa
    10441 agtgtataaa tatgttttca gttaaattag ccttgaggaa gaatagttat tatatgagtt
    10501 ttaagggtct tgataaatgt tgggacctac tctaaaactc atgaggttcc tcactgatca
    10561 atactcttgc attgtatttt atttctcaaa ataatgaaat ttttatggaa aagctcaatt
    10621 agtggaatct tttattcaat attcccttac tcttctttct tccttccttc tttctttcct
    10681 ttcttccttc cctccctcct tccttcctag ttctttcatt ttttcaggca agaagcttcc
    10741 ttaataatat agtttctaca gagctttctt acttattttt ctaataatgg atataacagt
    10801 gtgataaagt ttaaatgctc cgtgagcaaa actcttatca aacaactgaa gaaatacact
    10861 gctgattaac cttttccttg gtcttgaatt gtctccagca tatttgctag tgactaaacc
    10921 attaatggat cttattagta taaacacatg tctgtgtggt tatcaacagt gttgtattat
    10981 tgtcacattt atttacattt ttggtcaaaa atataaacat gcgtgtgtga agcagtctgt
    11041 tttatagagt gagcatatat gtaatcgttt tcatataatt gaaaatgtgc tgtgtgttgc
    11101 attttattct catgtcgact tttctactat gtatacaccg attgtctcgg tgcctttttc
    11161 cttttgtata attgtattac atacagaaat tgctgacctc tgtgaaaaca gtgtaaaaaa
    11221 agttaaaatt attattccga tgagatttta atggcagttt aattttccag agaataaatt
    11281 ttgtgtgggt taaaagttat ttttcttcac ctttatgtaa aaaatctgtt tacgttttag
    11341 tactcacatg gaatgggtaa acatctgact tcattgatga tgatacataa acatcagtcc
    11401 taattcttgt cattgacaaa taaatgttaa tatgactgtt acacaaaata aaataatgtc
    11461 aattacataa ggaaatatca aatggatata tgtacatgtt atttagatct tgggtttgaa
    11521 aggaatctca tctaaatgtg ctataaaata ttactctcaa aaggtttggg tcatattatt
    11581 ttctttaatt gtaagtcaaa tctggcttcc aaagaaaata gacttacaaa tgcaaacatt
    11641 taaaaatatt ctcctttaaa gttcatataa atcacaaatt aaactaaata gctttaaaat
    11701 gatctcagta tcttcagctg gaatatgaac tgttacctag tttgttcttt cacttaatgt
    11761 gaatttgttg tcattcatat cagtattgct atcctgttta acaaaataat tttctctttg
    11821 ccaatagtag tttgttgatt tttaattctt tttcattagc tagttgtatt gtttccctaa
    11881 gacctggagt ttagatattt ttcatatttc attaccttgt ccattttact tccagcaaca
    11941 aagatttata acaacagact taacaagtac tattttgcat atgaaaaaga atttaaacgt
    12001 ttggtgctct aaaactgaaa gaaattaaag ttgctgcatc atctaa
//
