LOCUS       NM_152592              13557 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens spectrin repeat containing nuclear envelope family
            member 3 (SYNE3), transcript variant 1, mRNA.
ACCESSION   NM_152592 NR_026779 XM_005267377
VERSION     NM_152592.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Cruz,V.E., Esra Demircioglu,F. and Schwartz,T.U.
  TITLE     Structural Analysis of Different LINC Complexes Reveals Distinct
            Binding Modes
  JOURNAL   J Mol Biol 432 (23), 6028-6041 (2020)
   PUBMED   33058875
  REMARK    GeneRIF: Structural Analysis of Different LINC Complexes Reveals
            Distinct Binding Modes.
REFERENCE   2  (bases 1 to 13557)
  AUTHORS   Liao,L., Zhang,L., Yang,M., Wang,X., Huang,W., Wu,X., Pan,H.,
            Yuan,L., Huang,W., Wu,Y. and Guan,J.
  TITLE     Expression profile of SYNE3 and bioinformatic analysis of its
            prognostic value and functions in tumors
  JOURNAL   J Transl Med 18 (1), 355 (2020)
   PUBMED   32948197
  REMARK    GeneRIF: Expression profile of SYNE3 and bioinformatic analysis of
            its prognostic value and functions in tumors.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 13557)
  AUTHORS   Del Real,A., Lopez-Delgado,L., Sanudo,C., Garcia-Ibarbia,C.,
            Laguna,E., Perez-Campo,F.M., Menendez,G., Alfonso,A., Fakkas,M.,
            Garcia-Montesinos,B., Valero,C., Perez-Nunez,M.I. and Riancho,J.A.
  TITLE     Long Noncoding RNAs as Bone Marrow Stem Cell Regulators in
            Osteoporosis
  JOURNAL   DNA Cell Biol 39 (9), 1691-1699 (2020)
   PUBMED   32700968
  REMARK    GeneRIF: Long Noncoding RNAs as Bone Marrow Stem Cell Regulators in
            Osteoporosis.
REFERENCE   4  (bases 1 to 13557)
  AUTHORS   Yang,Q., Li,X., Zhou,Y., Fu,W., Wang,J. and Wei,Q.
  TITLE     A LINC00341-mediated regulatory pathway supports chondrocyte
            survival and may prevent osteoarthritis progression
  JOURNAL   J Cell Biochem 120 (6), 10812-10820 (2019)
   PUBMED   30672021
  REMARK    GeneRIF: LINC00341 interacts with miR-141 to suppress its
            functional binding to the 3'-untranslated region of YAF2 messenger
            RNA whereby supporting chondrocyte survival and preventing
            osteoarthritis progression.
REFERENCE   5  (bases 1 to 13557)
  AUTHORS   Ji,Y., Jiang,J., Huang,L., Feng,W., Zhang,Z., Jin,L. and Xing,X.
  TITLE     Sperm-associated antigen 4 (SPAG4) as a new cancer marker interacts
            with Nesprin3 to regulate cell migration in lung carcinoma
  JOURNAL   Oncol Rep 40 (2), 783-792 (2018)
   PUBMED   29901114
  REMARK    GeneRIF: SPAG4, in cooperation with Nesprin3, has a fundamental
            pathological function in the migration of lung carcinoma cells.
REFERENCE   6  (bases 1 to 13557)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 13557)
  AUTHORS   Nery,F.C., Zeng,J., Niland,B.P., Hewett,J., Farley,J., Irimia,D.,
            Li,Y., Wiche,G., Sonnenberg,A. and Breakefield,X.O.
  TITLE     TorsinA binds the KASH domain of nesprins and participates in
            linkage between nuclear envelope and cytoskeleton
  JOURNAL   J Cell Sci 121 (Pt 20), 3476-3486 (2008)
   PUBMED   18827015
  REMARK    GeneRIF: association with human nesprin-3 appeared to be stronger
            for torsinADeltaE than for torsinA. TorsinA also associated with
            the KASH domains of nesprin-1 and -2
REFERENCE   8  (bases 1 to 13557)
  AUTHORS   Stewart-Hutchinson,P.J., Hale,C.M., Wirtz,D. and Hodzic,D.
  TITLE     Structural requirements for the assembly of LINC complexes and
            their function in cellular mechanical stiffness
  JOURNAL   Exp Cell Res 314 (8), 1892-1905 (2008)
   PUBMED   18396275
REFERENCE   9  (bases 1 to 13557)
  AUTHORS   Wilhelmsen,K., Litjens,S.H., Kuikman,I., Tshimbalanga,N.,
            Janssen,H., van den Bout,I., Raymond,K. and Sonnenberg,A.
  TITLE     Nesprin-3, a novel outer nuclear membrane protein, associates with
            the cytoskeletal linker protein plectin
  JOURNAL   J Cell Biol 171 (5), 799-810 (2005)
   PUBMED   16330710
REFERENCE   10 (bases 1 to 13557)
  AUTHORS   Heilig,R., Eckenberg,R., Petit,J.L., Fonknechten,N., Da Silva,C.,
            Cattolico,L., Levy,M., Barbe,V., de Berardinis,V., Ureta-Vidal,A.,
            Pelletier,E., Vico,V., Anthouard,V., Rowen,L., Madan,A., Qin,S.,
            Sun,H., Du,H., Pepin,K., Artiguenave,F., Robert,C., Cruaud,C.,
            Bruls,T., Jaillon,O., Friedlander,L., Samson,G., Brottier,P.,
            Cure,S., Segurens,B., Aniere,F., Samain,S., Crespeau,H., Abbasi,N.,
            Aiach,N., Boscus,D., Dickhoff,R., Dors,M., Dubois,I., Friedman,C.,
            Gouyvenoux,M., James,R., Madan,A., Mairey-Estrada,B., Mangenot,S.,
            Martins,N., Menard,M., Oztas,S., Ratcliffe,A., Shaffer,T.,
            Trask,B., Vacherie,B., Bellemere,C., Belser,C., Besnard-Gonnet,M.,
            Bartol-Mavel,D., Boutard,M., Briez-Silla,S., Combette,S.,
            Dufosse-Laurent,V., Ferron,C., Lechaplais,C., Louesse,C.,
            Muselet,D., Magdelenat,G., Pateau,E., Petit,E.,
            Sirvain-Trukniewicz,P., Trybou,A., Vega-Czarny,N., Bataille,E.,
            Bluet,E., Bordelais,I., Dubois,M., Dumont,C., Guerin,T.,
            Haffray,S., Hammadi,R., Muanga,J., Pellouin,V., Robert,D.,
            Wunderle,E., Gauguet,G., Roy,A., Sainte-Marthe,L., Verdier,J.,
            Verdier-Discala,C., Hillier,L., Fulton,L., McPherson,J.,
            Matsuda,F., Wilson,R., Scarpelli,C., Gyapay,G., Wincker,P.,
            Saurin,W., Quetier,F., Waterston,R., Hood,L. and Weissenbach,J.
  TITLE     The DNA sequence and analysis of human chromosome 14
  JOURNAL   Nature 421 (6923), 601-607 (2003)
   PUBMED   12508121
  REMARK    Erratum:[Nature. 2023 Aug;620(7973):E17. doi:
            10.1038/s41586-023-06403-y.. PMID: 37491470]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL133467.4 and AL138539.7.
            
            On or before Jun 15, 2020 this sequence version replaced
            XM_005267377.4, NM_152592.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.9606.1,
                                           SRR11853560.9243.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682763.1/ ENSP00000507501.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AL133467.4         27784-27838         c
            56-213              AL138539.7         127441-127598       c
            214-386             AL138539.7         119558-119730       c
            387-696             AL138539.7         117694-118003       c
            697-858             AL138539.7         108940-109101       c
            859-1206            AL138539.7         107140-107487       c
            1207-1343           AL138539.7         104010-104146       c
            1344-1518           AL138539.7         101694-101868       c
            1519-1701           AL138539.7         97672-97854         c
            1702-1845           AL138539.7         96248-96391         c
            1846-1980           AL138539.7         94918-95052         c
            1981-2142           AL138539.7         91677-91838         c
            2143-2315           AL138539.7         91375-91547         c
            2316-2445           AL138539.7         90796-90925         c
            2446-2607           AL138539.7         88583-88744         c
            2608-2757           AL138539.7         85023-85172         c
            2758-2796           AL138539.7         83842-83880         c
            2797-13557          AL138539.7         59029-69789         c
FEATURES             Location/Qualifiers
     source          1..13557
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.13"
     gene            1..13557
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /note="spectrin repeat containing nuclear envelope family
                     member 3"
                     /db_xref="GeneID:161176"
                     /db_xref="HGNC:HGNC:19861"
                     /db_xref="MIM:610861"
     exon            1..55
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            56..213
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2997
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nesprin-3; nuclear envelope spectrin repeat protein 3;
                     long intergenic non-protein coding RNA 341; KASH
                     domain-containing protein 3; uncharacterized protein
                     C14orf113"
                     /codon_start=1
                     /product="nesprin-3 isoform 1"
                     /protein_id="NP_689805.3"
                     /db_xref="CCDS:CCDS9935.1"
                     /db_xref="GeneID:161176"
                     /db_xref="HGNC:HGNC:19861"
                     /db_xref="MIM:610861"
                     /translation="MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARL
                     WETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMT
                     HCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLL
                     HNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQV
                     AREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESL
                     ETLEEQSAGVIRNTSPLGAEKITGELEEMRKVLEKLRALWEEEEERLRGLLRSRGAWE
                     QQIKQLEAELSEFRMVLQRLAQEGLQPAAKAGTEDELVAHWRRYSATRAALASEEPRV
                     DRLQAQLKELIVFPHNLKPLSDSVIATIQEYQSLKVKSARLRNAAAVELWQHFQRPLQ
                     DLQLWKALAQRLLEVTASLPDLPSLHTFLPQIEAALMESSRLKELLTMLQLKKDLLIG
                     IFGQERATALLEQVAGSMRDRDLLHNSLLQRKSKLQSLLAQHKDFGAAFEPLQRKLLD
                     LQVRVQAEKGLQRDLPGKQAQLSRLQGLQEEGLDLGAQMEAARPLVQENPNHQHKMDQ
                     LSSDFQALQRSLEDLVDRCRQSVQEHCTFSHQLLELRQWIVVTTQKLEAHRGEAGPGD
                     AESQEAEFERLVAEFPEKEAQLSLVEAQGWLVMEKSSPEGAAVVQEELRELAESWRAL
                     RLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQE
                     EEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQ
                     HLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWR
                     GLGSLFRRACCVALPLQLLLLLFLLLLFLLPIREEDRSCTLANNFARSFTLMLRYNGP
                     PPT"
     exon            214..386
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            387..696
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            697..858
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1206
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1343
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1518
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1701
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1845
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1980
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2142
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2315
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2445
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2607
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2757
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2796
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..13557
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      13529..13534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /note="hexamer: AATAAA"
     polyA_site      13557
                     /gene="SYNE3"
                     /gene_synonym="C14orf139; C14orf49; KASH3; LINC00341;
                     NCRNA00341; Nesp3; NET53"
                     /note="major polyA site"
ORIGIN      
        1 aaggaggctc ggccccagct acccgcggcg ccgcgctcct ccacgctccg ggaggcccct
       61 gcaggtgcca tgactcagca gccccaggac gactttgaca ggagcgtgga ggatgcccag
      121 gcatggatga aggctgtgca ggaccagctg caggtcaatg acaacacgca gggaccccgc
      181 gcggccctgg aggccaggct gtgggagacc gagaaaatat gccagctgga gcccgagggg
      241 cgtgtgaggg tggacctcgt gctacggatg gctgaagccc tcttggcatg ctgccctggg
      301 gaccagaagc ccgggatcct ggcccggctg aaggacatca aggcccaatg ggaggagaca
      361 gtcacctaca tgactcactg tcacagccgc atcgagtggg tgtggctgca ctggagcgag
      421 tacctgctgg cccgagatga gttctaccgc tggttccaga agatgatggt cacactggag
      481 ccccacatcg agctccagct gggcctgaag gagaagcagt ggcagctgag ccacgcccag
      541 gtgctgctgc acaacgtgga caaccaggcg gtgctcctgg accggctgct ggaggaggca
      601 gcctccctgt tcaacaggat cggggacccc agcgtggacg aagatgccca gaagagaatg
      661 aaggctgagt acgatgcagt gaaggccaag gcccagaagc gtgtagatct gctggagcag
      721 gtggcccggg agcatgagga gtaccaggca ggtgtggacg agttccaact gtggctgaag
      781 gcggtggtgg agaaggtgaa tggctgcctg gggcggaact gcaagctgcc catcacgcag
      841 cgcctctcca cactgcagga cattgccaaa gattttccca ggggcgagga gtctctggag
      901 acgctggagg agcagtctgc gggtgtcatt cggaacacct ctcctttggg tgcagagaag
      961 atcaccggag aactggaaga gatgaggaaa gttctggaga agctgcgcgc cctctgggag
     1021 gaggaggagg agcggctgcg gggcctgctc cggtccaggg gagcctggga gcagcagatt
     1081 aagcagctgg aggctgagct cagtgagttc aggatggtcc tgcagaggct ggcccaggag
     1141 ggcctgcagc ctgcggcgaa agcggggacc gaggacgagc tggtggcaca ctggagacgc
     1201 tactcggcaa cacgggcggc gctggcctcg gaggagcccc gggtggaccg gctgcaagcc
     1261 cagctgaagg agctcatcgt cttccctcac aacctgaagc cactctctga tagtgtcatc
     1321 gctaccatcc aggagtatca gagcctgaag gtgaagagcg cgaggctgcg caatgccgcg
     1381 gcggtggagc tgtggcagca tttccagcgg cctctgcagg atctgcagct gtggaaggcc
     1441 ctggcccagc ggctcttgga ggtcactgcc agcctgccgg acctgccttc ccttcacacc
     1501 ttcctgcccc agatcgaggc agccctgatg gaaagctccc gcctgaaaga gctgctgacg
     1561 atgctgcagc tgaagaaaga cctcctgatt ggcatctttg gccaggagag agccacggca
     1621 ctcctggagc aggtggcagg ttccatgagg gacagagacc tgctgcataa cagcctcctg
     1681 cagaggaaaa gcaaactgca gagcctgctc gctcagcaca aagactttgg agcagctttt
     1741 gagcccctgc agaggaagct cttggacctc caggtcaggg tccaagccga gaaggggctt
     1801 cagcgggacc ttcctggaaa acaggcccag ctctcaaggt tgcaggggct gcaggaagag
     1861 gggctggact tgggggcaca gatggaggct gcaaggcctc tggtccagga gaaccccaac
     1921 caccagcaca aaatggacca gctttcctcc gacttccagg ccctgcagag gtctctggag
     1981 gaccttgtgg acaggtgtcg gcagagtgtg caggagcact gcaccttcag ccaccagctg
     2041 ctggagctgc ggcagtggat cgttgtgacc acgcaaaagc tggaggcaca ccggggagag
     2101 gcgggcccgg gggatgccga gtcccaagag gccgagtttg agaggctggt ggcagaattc
     2161 ccggagaagg aggcccagct gtccctggtg gaagcgcagg gctggctggt gatggagaag
     2221 tcttctccgg agggtgctgc cgtggtgcag gaggagctca gggagctggc agagtcgtgg
     2281 cgggccttga ggctgctgga agaaagtctg ctgagcctca tcagaaactg gcatctgcag
     2341 aggatggaag tggattcggg gaagaaaatg gttttcacca acaacatccc aaagtcagga
     2401 tttctcatca atcccatgga tcctattccc aggcatcgtc gacgcgcaaa tcttctacag
     2461 gaagaagaag ggagccatga agatttctcc cagctcctga ggaactttgg gcagtggttg
     2521 caggtggaaa actccaagct ggttagaatc atcgcaatga gaacttctac agctgaggac
     2581 ttgaggacca gaaaatcgaa gctgcaggag ctggaggctc gggtgccaga aggtcagcat
     2641 ctctttgaga acctccttcg tctcgggcca gcaaggggga cctcggatga gctggaagat
     2701 ctgcgctacc agtggatgct gtacaagtcc aagctgaagg actctggcca cctgctgaca
     2761 caaagttctc caggggagcc gactggattc caaaagactc ggcggtggcg aggactgggc
     2821 tccctcttcc ggagggcgtg ctgtgtggcg ctcccactgc agctgcttct gctgctgttc
     2881 ctcctcctgc tgttcctgct cccaatcagg gaagaggacc gcagctgcac cctggccaac
     2941 aacttcgccc gctccttcac gctcatgctg cgctacaatg gcccaccacc cacctaaccc
     3001 actggggcac acaggtgact ttctcctcca acctccatca gtcccagggc ccctcctgag
     3061 gatgctcgcc agggaaactg gaaacagggg cagggcaaag actgcagagc tgcatccata
     3121 tcagtgctgt gtcagcgttc ccagaagaca cttacttttt atacagtgtt gtcttagtct
     3181 atttatacta aatgtgtact acatgtgact agggaataat gtctagaatt tttatatgat
     3241 ctgtatgtaa atgctcagta tgtataattt catatacgag ttctatttgg agttgggaaa
     3301 attcctagca atggctccct ccaatgggat tttgtggtat tgttgacttg gtctgcagtc
     3361 aaccctagaa agcaagaaca ataacatgtc actttcatgg caacctcttc tccaaagcga
     3421 atcccagaat tcagatgtaa tgaaaggcct ctgagctgca acgtggaaga gcgccataga
     3481 ccctcacttg gtgcccagcg actcttgctt tatgcgtaat gagaacaagt atgcttgtct
     3541 agttttaaca accacaaaga gtagctgcca cgtgtcagaa gtagaaatgg caatgaccag
     3601 aggcaggttc aatgatagca gtgacaagtg acagagtgat atcgagggag aaaaagaaga
     3661 gctgcagaga gcagatcccc gaactaagaa agcacagctg tgtggaggca ttgcatgccg
     3721 gagccctaac cccagctctc agcggcttct gatcacacag acctatgagg actgtccctg
     3781 gtgatgacac cgagccccca tgaaacggtg agatcctgct ctgtgtgttt cattcaggga
     3841 gaaagggaag aagacaagct tgatttttaa gatttccatg cagaatggtg aggcacactc
     3901 ggtgggcagc acccactggc agtgcccagg aaagatgtgt ttcctctgtt gctcctggac
     3961 tgagttttca tcgtcgagag ctcgagctgc aggtgagaag ggaagcatct gccacgccag
     4021 gggccaggga tcgagggtcc aggacagagg tgagagaatc tgctttggag ttcagcctgt
     4081 ctcaagggct ctccttccgc ttcccagaaa agactctgga gacagcaggt ctatggggcc
     4141 aagaaggtct catgtgcatc cccatatggg actagggccc aggccaccca aaaccaggcc
     4201 ctctccagcc aggtgtaggc gagctgggct ccatgcacca cctgggctgg ggccgcggtg
     4261 actccatgtg agttcccgag gccagaaact catcccttcc ttgccaggga actctgggat
     4321 ggttcctaag tgctctacaa ctggatcatg tttttcctaa atcaacacta aattttcccc
     4381 tttaaaaaag tacttaatgc ccactctcta caaatggatt ttgtctgttc aactacaaat
     4441 acgaggggat ttcctcggac cttctgtccg gctttggaat cataaggtat tatagaccca
     4501 gaaaggccct ttaagtagag gggaaactga ggacctaaga gggcctgata cccagcacca
     4561 ttgctgtctc acacacactg accctttgcc tttcactgtg agctctgccc ttggggaccc
     4621 gggtcctagg caactaggac atttcaccgg caggaaaccc gtgctgccaa ggagagccct
     4681 tggggctgac tcagaaagcg tacttttttt tttcttttaa ctcgtctgta tttgttacaa
     4741 ctttaagcag aatgtgactt gggcactata tttccatcca caagcctggc tctgagttat
     4801 tattattatt gttattataa ttattttgag atggagtttc gctctttttg cccaggctgg
     4861 agtgcagtgg tgcgatctcg gctcactgca acctccgcct cccagattca agcgattctc
     4921 ctgcttcagc ctcccaaata gctgggatta caggcatctg ccaccatgcc cagctaattt
     4981 ttgtattttt agtagagtta gggttttacc atgttggcca ggctggtctc gaactcctga
     5041 cctcaggtga tccgcccacc tcagcgtccc aaagcactgg gattacaggc atgagccacc
     5101 atgcctggcc attttttttt ttttttgaat agctttatga taagctgtag gcttataatt
     5161 atgctcctcc aaacaacact tttctttgga aaacaagccc tgtagactct ccatccttcc
     5221 atcaagttcc ttccctcaag ctgcttcagt ttaacttatt tctacaagac tagtacatgc
     5281 agaattggca actacagggg atgaaaagtt caaaaagtag atcctacaac cctgtgactt
     5341 ggtttccaaa tatctagaga aatttgtgat ttcatggaag aagagaaagc acacttttct
     5401 ccagatatct ggaaaccaag ccatagggcc gttctctgca ggcgaccacc ttactggaaa
     5461 atcaggagct gagtgagccc aggcagccaa gcccagatgt ccagtcctgg aagtcagagc
     5521 agtggcaggg tgggtgggtg ggggggcctt gccaggtgtc cactttccta tgtcacaggt
     5581 gagaaagctg aggcccagaa tggggaagaa actgatccat ggccacacag tgagaggcag
     5641 caaggccaga gcccaactgg ccaccatggc cctcaggtgg taccctgctc attgtggtat
     5701 gatccgctca tgtcagcaac tacatgaaaa aaacagccac tgggcatgta tgcagcctgt
     5761 cacatatacc acatgttggg gaaggagaag ctggaacatg cgggcggggg tgggaatatc
     5821 tacctatatt ttttaaattt tgataatgat gccatcaagt ctgtttgttt gcataactgt
     5881 tttttaaaaa agaattgata ttttcagtat tttcatataa atatacttgt aatataacgt
     5941 aacagcccag ttactggtgc ttccaagcac aagtgcaatc tcctttgctt aaaggagagc
     6001 caggcaatta ttcttatgac tttagtttcc agcctcttta acagccatgc agagctttaa
     6061 ggtgctactt gagaaaggtg gcgctgtggc gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     6121 gtgtgtgtgt gtgtgtcaga gagagagaga ggagggaagg agaggaggga gaaaggacca
     6181 ccagtgggtc ggttgatgaa ttagttatcc ctgcttacct gtccctccca cccctttctt
     6241 tcttagcccg ttctctgcat gtggaaaggc acatgcagca cccagagggt gtttaaaaag
     6301 aaaagctggg agcgggaggg atgggcaggt actgacagca gacgaggcga ggagaccctg
     6361 ggaggtgccc agtggtggcg ggacagaggg aacaccgaca agggagacaa agaatgtgct
     6421 cgtttggtgc tcgtgggtgc ttgagcccct tgggcggcac tttgcagcct tgctttatgt
     6481 aattcccaca tcgacacctg cccccagggt ttgatactgt tttaccccca tgtttgagat
     6541 ggaaccctga ggcttaaaga tataaagacc cttgctcatg ctgaagtggg tccaaaccca
     6601 caacatgggg caatgaggac cccaaagccc aggctggagg ggggcttcag gctggttcta
     6661 ccaggttgca gtctctgtag cctctgggaa tggagttccc tgggcaagca ggcagaactc
     6721 tctgcccatc agctcgcctg ggacatggcc agagcggagc atccaaatgt ttccctgagg
     6781 gactgccctc agctctgaga tgagtcacca ccagcagcac attcccttgg gccatctatc
     6841 attatcttca cattgctcac ctggaagatg agcccctcta atgtctcatg atgaggtcct
     6901 cggtcatgaa tgacagaact ccacaccccc aatgcccagc acaggataag agcttcagct
     6961 gtggtttgtt gaattacatt aaaaacccaa aggcaaatgt gtttcctgac acccgactcc
     7021 cttccagagg aaatgggtca aggatgacct gacccaattc catctctcag gccccgtttg
     7081 cttcaggcag ctggctggag ttccctgcag ggaccggggg tccaaagatg cttgtctccg
     7141 ggcatcttca ggggaaggaa gctggggacc ctctgcaccc tgggggccaa ggagaatgac
     7201 aggcagaggg caggaaaagg cctgaaggcg ggtggtttgt gctggatgtt ccctaatgga
     7261 ttttacagct ggcaaactgg gaccggtggc atcatcctgc cctgccttgc aggggatggg
     7321 aggctggggc tgcccacagt tagggagtaa agaggggatc ttatctcttc cattagactg
     7381 gagccaggcc tgactgggaa gctgggaggc tttgcagaag ctttgaggaa ctgcacttta
     7441 tggtggtctg gctggagagg gcggacgggg aagtgggcca gagggagtca aaggccagag
     7501 gtgatgtcag gcttgattta ctgaactgtg tgcttaaaag tggttaagat gctaaatttt
     7561 atgttatgtt tattttacca caatttgagg gtgagaggag agagtgagag caaaagagac
     7621 agagagaaga gagagaaaag gaggaaggga gggagggagg gagggataga gagagagaaa
     7681 aagagaatgg gaatggcagg ctgtgccttc atggttccca actttcctct gaggacaagc
     7741 gctagtggca aagctttcac tgaccaaagg caagtgaaac aatacgaggg aacaacttga
     7801 gtttttcttt ccataggatt tattttgccc aaacgctggc tcttcattct gggattatgt
     7861 ccctcctact tcaagggtgc tcaatggcct ttaggaaatg agggataggt aaaggtggct
     7921 ctgtcttctt tgatggtttc tattgtgttt tgggagccct cattggacaa aataaaaagt
     7981 tgccaactct atgttggttt tgctccccat acagtattct tcctggaaaa acatgctgtc
     8041 tgaaagtttg ggaacccagg agatggttgg gagaagagag cagcttgagg attgcttagt
     8101 gcaggagtcc ccgagggaga gaaggaaaga gaaatgggaa gagggacatc gtggaaagtt
     8161 gtggcaactg ggttcagggg aggccaggag cctgccagct gtggcctcta aaaaaataaa
     8221 acccaggtgc tcactggccc tgccttccca ctggccattc ggatgctgtc cggggtctgc
     8281 ggctttctct cccctggttg gtgggcagaa gtgccctgtg cctctttgca tcttaatcca
     8341 atgcaacaag cattctccag cctgtgccag gctttgtgct gggagattcg gggtgaacaa
     8401 agcccagtcc ctgccctcag ggagcttgca gacgaagaaa agggtttgtg ccattgaaga
     8461 tgcccaacgg gattgcagtc attgcccttg actttgccca tctgcctttc cagagggggc
     8521 aggcccactg tcacagaaac tggaagagcc acacagggga gacagtggga gagctcggaa
     8581 cagggcatga gagtgtcttg ctgcctcttg gaggccacca ttgtgactat gaaccatacc
     8641 gggcagagga agtgggtcaa ggaggccagg gcagctgggc cgggcaggga gaggacctgg
     8701 gcatggcaga ggaggggaaa atagactgac ccagcctcag gaggtcagaa gtgtgttggg
     8761 ctgtgctggg tcacagaaca acacagcttg ggaggctcct ggaggcttca aggggaggct
     8821 taaggctaag gctgtggatg ttgattgtcg tgtccattac tagcccaatt attagtctgt
     8881 cttcctggta aaaatgtgtg gttggaaatg ccaaactgag aagcccactt ttccccactg
     8941 gggatcatct gggttggcgt gtccatgctc aaggataatc tcttgccctt tcctgccccc
     9001 tgcctaggat ttctccccag aggagaggag ctgagggacc cccccaggga ggctgtgcct
     9061 gtggctgtca ccctatgatg ccagcggctt cctttggatt ctgtaatcag agtgtttttc
     9121 accggtgtct gcagtgattg ccaggaagcc tctgtgtcca tagcccagcg ggatcctgcc
     9181 ctaattccct ctctcatggg cactcagcta attctcaccc caatctcatt gtcaccagtc
     9241 taggtcagca ttggagggag gtgtgggcaa ctacagccct tgctgcttcc aagacagaca
     9301 tcaaaatagt tgatcaccaa cctttcccac tccccactct ctgacccaat ggacctcaca
     9361 gtcctcaaaa ctactccaag ctactgccaa tcaatcattt tcttatgtga gcaaaacctg
     9421 tttgacatcc ttacacctgt accagccctc tcagaaagct ctcaattctg gtctcctttt
     9481 cttttttctt tttttttttt tgaatttatg ttaaaattag gatgttccca atcaaaaaat
     9541 catttttcct tcatctagta attgggtgag acaaactagc cccagaaagc agacaatggg
     9601 atgaactgcc aagtaggact tttaggggag gcaaaatgga ttaggaaatg ctaagttctc
     9661 tgttgaagga ggatacctgg attcttcagg ctgtcagagt tggagggctt ccatagactg
     9721 tcttgttcca actttgtacc cattttacag gttaggactc tgagacctca gcagttcttg
     9781 tttggccagt gagctcaggg agttagttcc aggagtgggg gtcggtctct gcacccactg
     9841 gatgacagct tgatactgct cagcactaga cagggttgag ttggaactct gctgctgatg
     9901 ggctgtgtga cattggtcca gttccttacc acctcccccc acctattggt aaggagggaa
     9961 gtcagccctg gagcgttcag tgctccctga aatattaggt atcctaggat tgtgcgcgtg
    10021 ggctcgcatc tggaagagaa atccctagat tcagccacga gtaacagaat ccctcctccc
    10081 ccgaccaact ttggcttaaa ccagtcagag actttacttt tctctcctcc tgagaagtct
    10141 agatataggc ggttgctggc atcgtctcct caggttatgg atgtcagagc caaggctttg
    10201 caactgtcct tcctttatat cacagtcact tcatggccca ggatgcttgc tgtacctcca
    10261 atttttcatc tgggttccag gtaggaagaa ggggagtgaa ctagacgaat ctgccctcct
    10321 cattagcagc tttcccagaa gctccaccag ccacttccgt tctcatctca ttggccagga
    10381 ctgtgtcaca tgttttcccc cacatctgca aggagtctgg gaacatgtgc cccaaaaaga
    10441 tgggggctct tctagtaaga atgaagggta gggtggcttg gagtggcact tcgctgtctc
    10501 tgctacaatt ggaggagaaa accagagttt gtcctgaccc tccatgcctt ggctgtatgc
    10561 tccttgtgtg gaactcgaag gcatcatcct tgcagtgtga gagtgacctg ccaccacggc
    10621 ctgccacctc ctctcagggg accctgagtc cttcaggtct acccaaaggc attgtagaca
    10681 ttcttcctgc aaggcttggg aaccgacaaa tagatctgcc cagcttgcca gagtttttgt
    10741 gcaggaacag cccctcccgt ctttgtcctg gcggtgagca cccagggcta agcttttgaa
    10801 cactttcttt gtgtttggat tcagcccagg caatgcatat ttgctttcat ttcttcttga
    10861 gcttgaggag ctcctgggtg caaatcttgg aaaatgagga tctctgagcc tttccaggcc
    10921 agctctttgt tttgtagcag acaattgagg ctttgaaaag gaaagtgggt gggggcaccc
    10981 cacaggtggc cctcatcacc caattgccag tgcctgcagg ctgcttcagc agaggcccag
    11041 agtcaaagag gacttaaaac cagctgtcgt ttctccctta gcttctgtgt atgagagaaa
    11101 cgacttctgt ttttcaaagt aagaacaagg aggaatttgt ttctaaaaga acattaaaac
    11161 acaggctcgt ggtctaaaag caaatggttc agcaggatgt tcagggcctt aaagcacagt
    11221 cagcaggact cagcatctcc cagcacctgc tctccggttg tcatggtaac atcatcccca
    11281 acccaaccac cttgtccagc cgagagacag caatcataag gagggacctc ggtttccccc
    11341 gaggatcctg ggcttccttt ctgaaacgct tgcttctgag ctcagcaacc aggaacacca
    11401 ggccagccca tccccagcac ctctgtggag atgagggaca aagtcctaca gtccctcttc
    11461 ctgttctgat gagaaaggga gggaagaaaa cataccccga gcgcctgcaa tatggtcatg
    11521 acactttcaa aaagcctgtg ctatggagtc atgatcagaa accagagtgt ggagagggtc
    11581 agcagcctgc ctcagagcag ccagctaggc ggggagtggt aaatttggga cttgtaccca
    11641 ggcatgactg gctccgagcc cagtgctcca ctctatggaa tgttccctgg gcctcagttg
    11701 ctttcctttc ctttgcaggc cgcgggctgc tgccactctg gcagctggtg agttagctgg
    11761 agggcaacat tccaaagcag gggcagcatg ctgctttcct cctgtgccca ctcctgcggg
    11821 gaagtccgtt gactcccacc gctgaaggga gctggcaaca ccaggatgag gtcccagggg
    11881 acgggagcag gtacccactg tctgtctacc ttcccactgg aaaagcacgg acaggccagc
    11941 ccttgcgggg gcaggcagag gacagagttg gctttgcgcg gtctctgcct gctgagcagt
    12001 tccaattcct ctcatgggag aaacaaggag gcagtcgctt gtgcatgttc cagaagtttt
    12061 actggggagg aggaagcgga cagaggaagc tgtgtgtgca tgtgaagggg tgggcagggt
    12121 gggagggatg cacgcgtatg tgagcatagc atgtgtgagt actacacaca tctccatgca
    12181 gaagcacaac tgggcagccc tggcttccag ctctgggctt cagcacaaca gacaccagcc
    12241 tgtggtctct cagaagccag ggagaccaca tcgggctcag gacgttttac ccaaagtcca
    12301 gagtttttat gcctctccct ggcattctcc ataaagaagg gaaggtcaga tgacccctta
    12361 gatctgtgtc atctgggaat ttccttgggc tggtttagac acgatgccct ctttttctca
    12421 ggatagcaga taacctgctt tgaaagaggg cttaattctg tgggtcctaa attttctcct
    12481 ttctctctct ctttctgtgt gtgtgtgttg ggaaaatggc aagtttccaa taccagcttt
    12541 ggaggaacga ttacgttttc cctccaattt caagtccgaa agaccagagc cctcattcca
    12601 aagcccccca cccagatgga ttttttcgtt tcatttgtca tccgtcccat gggagggccc
    12661 catgtctcct cagaacccat cctggaggca gcaggtcggg tagagtgagt ttggcctgct
    12721 catgacctcc acccctgaga ttgtgaacaa ggatgtctgg ggcgatgctg agaatgtttt
    12781 tgaagctgct cccagatgac gctgatgatc acaccagatt gagtgctgcg atcgccttga
    12841 gtccaacctc tgcataaacg aggttctcat aaacaagttc actctaccct aagctaagtc
    12901 tatgtgagca aacccacttc atcctttgta cctggagacc tggttacact aacctgatac
    12961 tgacctgttc atgtagctgg aatggtgtgt ttcatgcagt gtggaccaag caatggcatg
    13021 gggtgtgtgt gtgtgtgtgt gtgtgtctgt gtgtgtgtgt ttgtgtatgc gttcacactt
    13081 gtgtgtgtat atgtgcatgt agatgctgca taaatgattt ttgatgtcaa agacaaacac
    13141 attccattgt tttaaatatt ctattatgta aacaatacgc agagggacca tatctactct
    13201 tgtcatatta tttgtgatgg taaaacatgc atttgcaata aattaagctt tctgggaagg
    13261 caagcagtat tggagccaaa cgactgtctc ggaacatgtg tgtgttatct cggttcatat
    13321 caagtccaaa gctaatggag ccttccccgc catccaggga ggaacaccag gaccccggag
    13381 tttcttctta gtgctatatt ttaaagttgc attgacgttt tcctcccctt ccttttgtgc
    13441 aagttggaag tagcagtgtt ctaaaagatg gtttgacgtt tttgctgttg ttttatgttt
    13501 ttaaaaatgt atctgctttg tgtttggaaa taaaaatctc tattttggtc tatgaaa
//
