LOCUS       NM_004819               4077 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens symplekin scaffold protein (SYMPK), mRNA.
ACCESSION   NM_004819
VERSION     NM_004819.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Boreikaite,V., Elliott,T.S., Chin,J.W. and Passmore,L.A.
  TITLE     RBBP6 activates the pre-mRNA 3' end processing machinery in humans
  JOURNAL   Genes Dev 36 (3-4), 210-224 (2022)
   PUBMED   35177536
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Qin,Y., Huttlin,E.L., Winsnes,C.F., Gosztyla,M.L., Wacheul,L.,
            Kelly,M.R., Blue,S.M., Zheng,F., Chen,M., Schaffer,L.V., Licon,K.,
            Backstrom,A., Vaites,L.P., Lee,J.J., Ouyang,W., Liu,S.N., Zhang,T.,
            Silva,E., Park,J., Pitea,A., Kreisberg,J.F., Gygi,S.P., Ma,J.,
            Harper,J.W., Yeo,G.W., Lafontaine,D.L.J., Lundberg,E. and Ideker,T.
  TITLE     A multi-scale map of cell structure fusing protein images and
            interactions
  JOURNAL   Nature 600 (7889), 536-542 (2021)
   PUBMED   34819669
REFERENCE   3  (bases 1 to 4077)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4077)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 4077)
  AUTHORS   Gong,X., Liu,J., Zhang,X., Dong,F., Liu,Y. and Wang,P.
  TITLE     Rab11 Functions as an Oncoprotein via Nuclear Factor kappa B
            (NF-kappaB) Signaling Pathway in Human Bladder Carcinoma
  JOURNAL   Med Sci Monit 24, 5093-5101 (2018)
   PUBMED   30032159
  REMARK    GeneRIF: Research proved that high Rab11 expression enhances
            cellular multiplication and invasiveness of bladder cancer,
            possibly by regulating the NF-kappaB signaling pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4077)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     Complex protein interactions within the human polyadenylation
            machinery identify a novel component
  JOURNAL   Mol Cell Biol 20 (5), 1515-1525 (2000)
   PUBMED   10669729
REFERENCE   7  (bases 1 to 4077)
  AUTHORS   Faber,P.W., Barnes,G.T., Srinidhi,J., Chen,J., Gusella,J.F. and
            MacDonald,M.E.
  TITLE     Huntingtin interacts with a family of WW domain proteins
  JOURNAL   Hum Mol Genet 7 (9), 1463-1474 (1998)
   PUBMED   9700202
REFERENCE   8  (bases 1 to 4077)
  AUTHORS   Alwazzan,M., Hamshere,M.G., Lennon,G.G. and Brook,J.D.
  TITLE     Six transcripts map within 200 kilobases of the myotonic dystrophy
            expanded repeat
  JOURNAL   Mamm Genome 9 (6), 485-487 (1998)
   PUBMED   9585442
REFERENCE   9  (bases 1 to 4077)
  AUTHORS   Ueki,K., Ramaswamy,S., Billings,S.J., Mohrenweiser,H.W. and
            Louis,D.N.
  TITLE     Chromosomal localization to 19q13.3, partial genomic structure and
            5' cDNA sequence of the human symplekin gene
  JOURNAL   Somat Cell Mol Genet 23 (3), 229-231 (1997)
   PUBMED   9330635
REFERENCE   10 (bases 1 to 4077)
  AUTHORS   Keon,B.H., Schafer,S., Kuhn,C., Grund,C. and Franke,W.W.
  TITLE     Symplekin, a novel type of tight junction plaque protein
  JOURNAL   J Cell Biol 134 (4), 1003-1018 (1996)
   PUBMED   8769423
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB044963.1, BC006536.2,
            Y10931.1, BU752018.1 and AC092301.2.
            
            On May 17, 2019 this sequence version replaced NM_004819.2.
            
            Summary: This gene encodes a nuclear protein that functions in the
            regulation of polyadenylation and promotes gene expression. The
            protein forms a high-molecular weight complex with components of
            the polyadenylation machinery. It is thought to serve as a scaffold
            for recruiting regulatory factors to the polyadenylation complex.
            It also participates in 3'-end maturation of histone mRNAs, which
            do not undergo polyadenylation. The protein also localizes to the
            cytoplasmic plaques of tight junctions in some cell types.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK300252.1, Y10931.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245934.12/ ENSP00000245934.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-443               DB044963.1         120-562
            444-1238            BC006536.2         294-1088
            1239-4023           Y10931.1           1200-3984
            4024-4045           BU752018.1         21-42               c
            4046-4077           AC092301.2         21876-21907         c
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..4077
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /note="symplekin scaffold protein"
                     /db_xref="GeneID:8189"
                     /db_xref="HGNC:HGNC:22935"
                     /db_xref="MIM:602388"
     exon            1..90
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            91..207
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..3927
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /codon_start=1
                     /product="symplekin"
                     /protein_id="NP_004810.2"
                     /db_xref="CCDS:CCDS12676.2"
                     /db_xref="GeneID:8189"
                     /db_xref="HGNC:HGNC:22935"
                     /db_xref="MIM:602388"
                     /translation="MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAA
                     LITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKR
                     DIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDM
                     VSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPR
                     DHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQ
                     AYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAE
                     IARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQ
                     SDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLA
                     RLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRLSAQGQAISVVGSLSS
                     MSPLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAV
                     KRILRAEKAVACSGAAQVRIKILASLVTQFNSGLKAEVLSFILEDVRARLDLAFAWLY
                     QEYNAYLAAGASGSLDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVV
                     RKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMY
                     EKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALL
                     PQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLV
                     TRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLN
                     PIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSVKCDMKSIIKATNLCFAE
                     RNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWK
                     YPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQA
                     HIPNSIMTILEASGKQEPEAKEAPAGPLEEDDLEPLTLAPAPAPRPPQDLIGLRLAQE
                     KALKRQLEEEQKLKPGGVGAPSSSSPSPSPSARPGPPPSEEAMDFREEGPECETPGIF
                     ISMDDDSGLTEAALLDSSLEGPLPKETAAGGLTLKEERSPQTLAPVGEDAMKTPSPAA
                     EDAREPEAKGNS"
     exon            208..273
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            274..327
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            328..401
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            402..528
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            529..778
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            779..949
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1189
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1344
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1495
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1700
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1851
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..2087
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2169
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2283
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2431
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..2592
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2701
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2802
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2893
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..2995
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3183
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3360
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            3361..3456
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3789
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     exon            3790..4077
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4018..4023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /note="hexamer: AATAAA"
     polyA_site      4037
                     /gene="SYMPK"
                     /gene_synonym="Pta1; SPK; SYM"
                     /note="major polyA site"
ORIGIN      
        1 gacgaacgga agatggcggc ggcggcggcg gcggagtgta ggaagaggca ctgctgaggg
       61 ggcgcgaggg gaacggaggc cagagctgcg ctgacagcag ccatggcgag cggcagtgga
      121 gacagcgtca cccgtcggag cgtggcatca cagtttttca ctcaagagga ggggccgggc
      181 atcgatggca tgaccacctc agagagggtg gtggatcttc tgaaccaggc ggcgctgatc
      241 accaatgact caaagatcac agtgctcaaa caggtccagg agctgatcat caacaaagac
      301 cccacactac tggacaactt cctggatgag atcatcgcat tccaagcaga caagtcaatc
      361 gaagtgcgaa aatttgtcat cggcttcatc gaggaggcat gcaagcgaga cattgagttg
      421 ctgctgaaac tcattgcaaa cctcaacatg ctcttgaggg acgagaatgt gaacgtggtg
      481 aagaaggcta tcctcaccat gacccagctc tacaaggtgg ccctgcagtg gatggtaaag
      541 tcacgggtca ttagcgagct acaggaggcc tgctgggaca tggtatctgc catggcgggg
      601 gacatcatcc tgctattgga ctctgacaat gacggcatcc gcacccacgc catcaagttt
      661 gtggagggcc tcattgtcac cctgtcaccc cgcatggctg actcagagat accccgacgc
      721 caggagcatg atatcagcct ggaccgcatc cctcgtgacc acccctacat ccagtacaac
      781 gtgctatggg aagagggcaa ggcagccttg gagcagctgc ttaagttcat ggtgcaccct
      841 gccatctcct ccatcaacct gaccacagcg ctgggctccc ttgccaatat cgcccgccag
      901 agacccatgt tcatgtctga ggtgatccag gcctatgaaa ctctgcatgc caacctgccc
      961 ccgacgctgg ccaaatcgca ggtgagcagt gtgcgtaaga atctgaagct gcacctgttg
     1021 agtgtgctga agcacccggc ttccttggag ttccaggccc agatcaccac cctgctggtg
     1081 gacctgggca cacctcaggc cgagatcgcc cgcaacatgc cgagcagcaa ggacacccgc
     1141 aagcggcccc gcgatgactc ggactccaca ctcaagaaga tgaagctgga gcccaacctg
     1201 ggggaggacg atgaggacaa agacttggag ccaggcccgt cggggacctc gaaggcctca
     1261 gcgcagatct ccggccagtc agacacggac atcacagctg agttcctgca gcctctgctg
     1321 acgcctgata atgtggctaa tctggtcctc atcagcatgg tgtacctacc cgaggccatg
     1381 ccagcctcct tccaggccat ctacaccccc gtggagtcag caggcacgga agcccagatc
     1441 aagcacctgg ctcggctcat ggccacacag atgacagctg ccggactggg accaggtgta
     1501 gagcagacca aacagtgcaa ggaggagccc aaggaggaga aggtggtgaa gacagagagc
     1561 gtcctgatca agcggcgcct gtcagcccag ggccaagcca tctcggtggt gggttccctg
     1621 agctccatgt cccccctgga ggaagaggca ccgcaggcca agaggaggcc agagcccatt
     1681 atccctgtca ctcagccccg gctggcaggc gctggtgggc gcaagaaaat tttccgtctc
     1741 agcgacgtgc tgaagcccct taccgatgcc caggtggaag ccatgaagct gggcgctgtg
     1801 aagcggatcc tgcgggctga gaaggctgtg gcctgcagcg gggcagccca ggtccgcata
     1861 aagatcctgg ccagcctggt gacacagttc aactcgggcc tgaaggcgga ggtcctgtcc
     1921 ttcatcctgg aggatgtgcg ggcccgcctg gacctggcct tcgcctggct ctaccaggag
     1981 tacaacgcct acctggccgc aggtgcctcg ggctccctgg acaagtatga ggactgcctc
     2041 atccgcctgt tgtctggcct gcaggagaaa ccagaccaga aggatgggat cttcaccaag
     2101 gttgtgctgg aggcgccact catcacagag agtgccctgg aggtggtccg caagtactgc
     2161 gaggatgaga gtcgcaccta tctgggcatg tccacacttc gagacctgat cttcaagcgc
     2221 ccgtcccgcc agttccagta cctgcatgtc ctcctcgacc tcagctccca tgagaaggac
     2281 aaggtgcgct cccaggccct gctgttcatc aaacgcatgt atgagaagga gcagctgcgg
     2341 gagtatgtgg agaaatttgc cctcaactac ctgcagctcc tggtgcaccc caacccaccg
     2401 tctgtgctgt ttggagctga caaggacaca gaggtggcag caccctggac ggaggagaca
     2461 gtgaagcagt gtctgtacct ctacctggcc ctcctgcctc agaaccacaa gctgatccac
     2521 gaactggcgg ccgtgtacac tgaagccatc gccgacatca agcggacggt gctgagggtc
     2581 attgagcagc cgatccgagg aatgggcatg aactccccgg agctgctcct gctggtggaa
     2641 aattgtccca agggagcaga gacactggtc acgagatgtc tgcacagcct cacagacaaa
     2701 gtcccaccct ccccagagct ggtgaagcgg gtccgggatc tctaccacaa gcgactgcca
     2761 gacgtccgct tcctcatccc ggtgctcaat gggctggaga agaaagaggt gatccaggcc
     2821 ctgcctaaac tcatcaaact caaccccatc gtggtgaagg aagtcttcaa ccgcctgctg
     2881 ggcacccagc atggtgaggg aaactcagcc ttgtccccgc tgaaccctgg agagctcctg
     2941 atcgcattac acaacattga ctccgtgaag tgcgacatga aatccatcat caaagccacc
     3001 aacctgtgct ttgcggagcg gaacgtgtac acgtcagagg tgctggccgt ggtgatgcag
     3061 cagctgatgg agcagagccc cctgcccatg ctgctcatga ggaccgtcat ccagtccctg
     3121 accatgtacc cccgcctggg gggcttcgtc atgaacatcc tgtcccgcct catcatgaag
     3181 caggtgtgga agtaccccaa ggtgtgggag ggcttcatca agtgctgcca gcgcacaaag
     3241 ccccagagct tccaggtcat cctgcagctg ccgccccagc agctgggagc cgtctttgac
     3301 aagtgcccag agctccggga gcccctgctg gcccatgtcc gctccttcac cccccaccag
     3361 caagctcaca tccctaactc catcatgacc atcttggagg ccagcggcaa gcaggagcca
     3421 gaggccaagg aggcgcctgc ggggcccttg gaggaggatg atctggagcc cctgaccttg
     3481 gccccggccc cagcaccccg gccccctcag gacctcatcg gcctgcgact ggcccaggag
     3541 aaggccttaa agcggcagct ggaggaggaa cagaagctga agccgggagg agtgggagcc
     3601 ccctcctctt cctccccctc tccctctccg tcggcccggc caggcccgcc cccgtctgag
     3661 gaagccatgg atttccggga ggaggggcct gagtgcgaga ccccgggcat cttcatcagc
     3721 atggatgacg actcggggct gaccgaggcc gcgctgttgg actctagtct cgagggcccc
     3781 ctacccaagg agacggcagc gggcgggctg accttgaagg aggagcggag cccccagacc
     3841 ctcgcacctg ttggagaaga tgctatgaag actcccagcc cggctgccga ggacgccagg
     3901 gaacccgagg ccaaggggaa cagctgacgg ggctcgaggg ggaaaggggg tgggacaggg
     3961 actcggggct gggggacggg gcggggcttg acctgcgggt gctttgcctt aaaaagaaat
     4021 aaaagatgtg aacttgggca agttactgaa aagaaaaaaa aaaagggatg gcgggcc
//
