LOCUS       NM_021738               8306 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.
ACCESSION   NM_021738
VERSION     NM_021738.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Zhou,J., Que,Y., Pan,L., Li,X., Zhu,C., Jin,L. and Li,S.
  TITLE     Supervillin Contributes to LPS-induced Inflammatory Response in
            THP-1 Cell-derived Macrophages
  JOURNAL   Inflammation 45 (1), 356-371 (2022)
   PUBMED   34480249
  REMARK    GeneRIF: Supervillin Contributes to LPS-induced Inflammatory
            Response in THP-1 Cell-derived Macrophages.
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Yang,G., Hiruma,S., Kitamura,A., Kinjo,M., Mishra,M. and Uehara,R.
  TITLE     Molecular basis of functional exchangeability between ezrin and
            other actin-membrane associated proteins during cytokinesis
  JOURNAL   Exp Cell Res 403 (2), 112600 (2021)
   PUBMED   33862101
  REMARK    GeneRIF: Molecular basis of functional exchangeability between
            ezrin and other actin-membrane associated proteins during
            cytokinesis.
REFERENCE   3  (bases 1 to 8306)
  AUTHORS   Zhang,Z., Chen,Y., Wu,Y., Hao,Y., Zhao,X., Wang,X., Wang,Y., Xi,Y.
            and Zhang,X.
  TITLE     A twin-pair analysis indicates congenital scoliosis is associated
            with allele-specific methylation in the SVIL gene
  JOURNAL   Mol Med Rep 22 (3), 2093-2100 (2020)
   PUBMED   32582973
  REMARK    GeneRIF: A twinpair analysis indicates congenital scoliosis is
            associated with allelespecific methylation in the SVIL gene.
REFERENCE   4  (bases 1 to 8306)
  AUTHORS   Hedberg-Oldfors,C., Meyer,R., Nolte,K., Abdul Rahim,Y.,
            Lindberg,C., Karason,K., Thuestad,I.J., Visuttijai,K., Geijer,M.,
            Begemann,M., Kraft,F., Lausberg,E., Hitpass,L., Gotzl,R.,
            Luna,E.J., Lochmuller,H., Koschmieder,S., Gramlich,M., Gess,B.,
            Elbracht,M., Weis,J., Kurth,I., Oldfors,A. and Knopp,C.
  TITLE     Loss of supervillin causes myopathy with myofibrillar
            disorganization and autophagic vacuoles
  JOURNAL   Brain 143 (8), 2406-2420 (2020)
   PUBMED   32779703
  REMARK    GeneRIF: Loss of supervillin causes myopathy with myofibrillar
            disorganization and autophagic vacuoles.
            Erratum:[Brain. 2021 Apr 12;144(3):e34. doi:
            10.1093/brain/awaa412.. PMID: 33313686]
REFERENCE   5  (bases 1 to 8306)
  AUTHORS   Zhao,C., Zhao,Z., Wang,Z., Hu,L., Wang,H. and Fang,Z.
  TITLE     Supervillin promotes tumor angiogenesis in liver cancer
  JOURNAL   Oncol Rep 44 (2), 674-684 (2020)
   PUBMED   32468064
  REMARK    GeneRIF: Supervillin promotes tumor angiogenesis in liver cancer.
REFERENCE   6  (bases 1 to 8306)
  AUTHORS   Ting,H.J., Yeh,S., Nishimura,K. and Chang,C.
  TITLE     Supervillin associates with androgen receptor and modulates its
            transcriptional activity
  JOURNAL   Proc Natl Acad Sci U S A 99 (2), 661-666 (2002)
   PUBMED   11792840
  REMARK    GeneRIF: Supervillin associates with androgen receptor and
            modulates its transcriptional activity.
REFERENCE   7  (bases 1 to 8306)
  AUTHORS   Kim,M., Jiang,L.H., Wilson,H.L., North,R.A. and Surprenant,A.
  TITLE     Proteomic and functional evidence for a P2X7 receptor signalling
            complex
  JOURNAL   EMBO J 20 (22), 6347-6358 (2001)
   PUBMED   11707406
REFERENCE   8  (bases 1 to 8306)
  AUTHORS   Wulfkuhle,J.D., Donina,I.E., Stark,N.H., Pope,R.K.,
            Pestonjamasp,K.N., Niswonger,M.L. and Luna,E.J.
  TITLE     Domain analysis of supervillin, an F-actin bundling plasma membrane
            protein with functional nuclear localization signals
  JOURNAL   J Cell Sci 112 (Pt 13), 2125-2136 (1999)
   PUBMED   10362542
REFERENCE   9  (bases 1 to 8306)
  AUTHORS   Pope,R.K., Pestonjamasp,K.N., Smith,K.P., Wulfkuhle,J.D.,
            Strassel,C.P., Lawrence,J.B. and Luna,E.J.
  TITLE     Cloning, characterization, and chromosomal localization of human
            superillin (SVIL)
  JOURNAL   Genomics 52 (3), 342-351 (1998)
   PUBMED   9867483
REFERENCE   10 (bases 1 to 8306)
  AUTHORS   Pestonjamasp,K.N., Pope,R.K., Wulfkuhle,J.D. and Luna,E.J.
  TITLE     Supervillin (p205): A novel membrane-associated, F-actin-binding
            protein in the villin/gelsolin superfamily
  JOURNAL   J Cell Biol 139 (5), 1255-1269 (1997)
   PUBMED   9382871
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF109135.1, AF051851.1,
            AL160060.11, BX457194.2, AF051850.1, BC092440.1, AI028650.1 and
            AL158167.15.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_021738.2.
            
            Summary: This gene encodes a bipartite protein with distinct amino-
            and carboxy-terminal domains. The amino-terminus contains nuclear
            localization signals and the carboxy-terminus contains numerous
            consecutive sequences with extensive similarity to proteins in the
            gelsolin family of actin-binding proteins, which cap, nucleate,
            and/or sever actin filaments. The gene product is tightly
            associated with both actin filaments and plasma membranes,
            suggesting a role as a high-affinity link between the actin
            cytoskeleton and the membrane. The encoded protein appears to aid
            in both myosin II assembly during cell spreading and disassembly of
            focal adhesions. Several transcript variants encoding different
            isoforms of supervillin have been described. [provided by RefSeq,
            Apr 2016].
            
            Transcript Variant: This variant (2) is alternatively spliced in
            the 5' portion of the gene, resulting in the longer,
            muscle-specific isoform (2) which has a different amino-terminus
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript that matched the
            reference genome was available for the full length of the gene. The
            extent of this transcript is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF109135.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355867.9/ ENSP00000348128.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-790               AF109135.1         2-791
            791-1240            AF051851.1         139-588
            1241-1241           AL160060.11        36738-36738         c
            1242-1579           AF051851.1         590-927
            1580-1888           AF109135.1         1581-1889
            1889-2590           AL160060.11        18332-19033         c
            2591-2761           AL160060.11        17805-17975         c
            2762-4454           AF109135.1         2763-4455
            4455-4932           BX457194.2         304-781
            4933-7718           AF051850.1         3353-6138
            7719-8294           BC092440.1         740-1315
            8295-8296           AI028650.1         10-11               c
            8297-8306           AL158167.15        168885-168894       c
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p11.23"
     gene            1..8306
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /note="supervillin"
                     /db_xref="GeneID:6840"
                     /db_xref="HGNC:HGNC:11480"
                     /db_xref="MIM:604126"
     exon            1..552
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            553..610
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            611..702
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            703..760
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     CDS             753..7397
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /note="isoform 2 is encoded by transcript variant 2;
                     membrane-associated F-actin binding protein p205;
                     archvillin; p205/p250"
                     /codon_start=1
                     /product="supervillin isoform 2"
                     /protein_id="NP_068506.2"
                     /db_xref="CCDS:CCDS7164.1"
                     /db_xref="GeneID:6840"
                     /db_xref="HGNC:HGNC:11480"
                     /db_xref="MIM:604126"
                     /translation="MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMR
                     ASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKA
                     ERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESS
                     RDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAH
                     DLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPS
                     TGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQP
                     APVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECS
                     WVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALE
                     QSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTE
                     RTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPA
                     SRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGP
                     GLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDD
                     EEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPIT
                     TEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNE
                     GKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVT
                     LGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSS
                     IENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEA
                     EASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLD
                     LRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDS
                     SEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRL
                     SRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRG
                     AANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLE
                     SDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMP
                     RSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEY
                     TEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNS
                     AVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKA
                     KASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKED
                     ELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKE
                     VTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTE
                     HNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTI
                     LDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVK
                     WKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGA
                     QVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVA
                     CHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSS
                     SKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAAT
                     EFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARI
                     RWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEIT
                     EMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALD
                     MTRDEYNALPAWKQVNLKKAKGLF"
     exon            761..912
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1579
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1660
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..2590
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2761
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2796
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2858
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2998
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3094
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3338
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            3339..3915
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            3916..4141
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4142..4268
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4269..4416
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4417..4506
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4507..4593
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4594..4771
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4772..4944
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            4945..5100
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            5101..5237
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            5238..5385
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            5386..5531
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            5532..5707
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            5708..5852
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            5853..6129
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            6130..6281
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            6282..6387
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            6388..6595
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            6596..6729
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            6730..6885
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            6886..7029
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            7030..7154
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            7155..7310
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     exon            7311..8306
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8189..8194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /note="hexamer: AATAAA"
     polyA_site      8211
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /note="major polyA site"
     regulatory      8260..8265
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /note="hexamer: AATAAA"
     regulatory      8274..8279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SVIL"
                     /gene_synonym="MFM10"
                     /note="hexamer: AATAAA"
     polyA_site      8287
                     /gene="SVIL"
                     /gene_synonym="MFM10"
     polyA_site      8306
                     /gene="SVIL"
                     /gene_synonym="MFM10"
ORIGIN      
        1 actgctctga ctgcgtcctc cccaggctga agtgtcctca ggtttcatgt ggatttgccc
       61 agtaatggat ggctccaccg aggccaggtt ggggaagttc agcctcgagg acactagggg
      121 acgaaaaact tcaaaaagaa gactcttttt attaatattt tggcgttctt tttggagagg
      181 aacttttggg atgtagcctt tacctgtttc ctgaatttga catccaaaaa gccttttgga
      241 aaacaccatt ctaaatttat cctgttctga atcatcatcg cttctgatag atgcagttgc
      301 ttgctgacat cgtgatcttt gattttgtac aacgctattt gggctaactt ttgggatttt
      361 actttgtgct tagaatttga aagtagtatt cattggaaag agactccaag aaaatactcg
      421 aagacatcaa aagctggatt cgagggtata acgtgttttg ttttgttttt taaattagaa
      481 aaataaggaa ttgcagtgtg attttctttc cccctttaac ttagaggaac gaggattata
      541 cagaaatttg aaatttcaac aagaaaaact taagcttcct tggattccca cgtcaaagga
      601 aagtttcaag ctttcagaag gagttctcac tcgaagataa agaacagctc gctaaccacg
      661 aaagaggaat cgatgctcag cttttagttg cacttcctaa agttgcagaa ttaagacaaa
      721 tctttgaacc aaagaagaaa gaattcttag aaatgaaaag aaaagaaaga attgccaggc
      781 gcctggaagg gattgaaaat gacactcagc ccatcctctt gcagagctgc acaggattgg
      841 tgactcaccg cctgctggag gaagacaccc ctcgatacat gagagccagc gaccctgcca
      901 gcccccacat cggccgatca aatgaagagg aggaaacttc tgattcttct ctagaaaagc
      961 aaactcgatc caaatactgc acagaaacct ccggtgtcca cggtgactca ccctatggtt
     1021 cgggtaccat ggacacccac agtctggagt ccaaagccga aagaattgca aggtacaaag
     1081 cagaaagaag gcgacagctg gcagagaagt atgggctgac tctggatccc gaggccgact
     1141 ccgagtattt atcccgctat accaagtcca ggaaggagcc tgatgctgtc gagaagcggg
     1201 gaggaaaaag tgacaaacag gaagagtcaa gcagagatgc tagttctctg taccccggga
     1261 ccgagacgat ggggctcagg acctgtgccg gtgaatccaa ggactatgcc ctccatgtgg
     1321 gtgacggctc ttccgacccg gaggtgctgc tgaacataga aaaccaaaga cgaggtcaag
     1381 agctgagtgc cacccggcag gcccatgacc tgtccccagc agccgagagt tcctcgacct
     1441 tctctttctc tgggcgagac tcctccttca ctgaagtgcc acggtccccc aagcacgccc
     1501 acagctcctc cctgcagcag gcagcctccc ggagcccctc ctttggtgac ccacagctat
     1561 cccctgaggc ccgacccagt acagggaaac ccaaacatga gtggtttctc cagaaagatt
     1621 ccgaagggga cacaccttca cttatcaact ggccttccag agttaaagtt agagaaaaat
     1681 tggtgaaaga ggaaagtgct cgaaacagcc ctgaactcgc ctcagagtcc gtaactcaga
     1741 ggagacacca gccagcgcca gtccattacg tgtcatttca gtctgagcac tcagcctttg
     1801 atagggtccc cagcaaggca gcaggctcta cacgacagcc aatccgtggc tatgtccaac
     1861 ccgcagatac cggtcacacc gccaagctag tgacgccaga aaccccagaa aatgcatctg
     1921 agtgtagctg ggtagcatca gccacccaga atgtccccaa acctcccagc ttgacggttc
     1981 tagaaggtga cggaagggat agcccagttc tccatgtctg cgagtcaaaa gcagaagaag
     2041 aagaagggga aggagaagga gaagaaaaag aagaagatgt gtgcttcact gaagctctcg
     2101 agcaaagcaa gaaaacccta ctggctttgg agggtgatgg gctagtgaga agcccagaag
     2161 atccctctag aaatgaggac tttggtaagc ctgctgtgag cacagtcacc ttagagcatc
     2221 agaaggaact ggaaaacgtg gcacaacccc ctcaagctcc gcaccagccc actgagagga
     2281 caggcaggag cgagatggtt ctctacattc aaagtgagcc tgtgtcccaa gacgccaaac
     2341 caactggtca caacagggaa gcctcgaaaa agcgcaaggt ccgtacccgc tctctgtcag
     2401 atttcacagg cccccctcag ctccaggcct tgaagtataa ggacccagct tccaggagag
     2461 agctggagct gcccagctcc aagaccgaag ggccttatgg ggagatcagc atgctggaca
     2521 caaaagtctc tgtcgcccag ctccgaagtg cgttcctggc atctgccaac gcctgcagga
     2581 gacctgaact caaatcacgg gtggagaggt cggctgaagg acctggcttg cccaccggtg
     2641 tggaacggga gagagggtcc cggaaaccaa gacgctattt ttctcctggt gaaagtagaa
     2701 aaacttccga gagatttaga acccaaccta taacttcagc agaacgaaag gaatcggata
     2761 ggtgcacttc acattcagaa acgccaactg tcgatgatga agaaaaggtg gatgaacgag
     2821 ccaagctgag cgtcgccgcc aagaggttgc ttttcaggga gatggaaaaa tcttttgatg
     2881 aacaaaatgt tccaaagcga cgctcaagaa acacagctgt ggagcagagg ctacgccgtc
     2941 tgcaggacag gtccctcacc cagcccatca ccactgaaga ggtggtcatc gcagccactg
     3001 aacctatccc cgcttcgtgt tctgggggca cccaccctgt aatggcgaga cttcctagcc
     3061 ccactgtagc taggagcgct gtgcagcctg ccagattgca ggcctctgct caccaaaagg
     3121 ccttagccaa ggaccagaca aatgagggca aagagcttgc tgagcaagga gaacctgatt
     3181 cctccactct aagcttggcc gaaaagttgg ccttgtttaa caaattgtcc cagccagtct
     3241 caaaagcgat ttctacccgg aacagaatag acacgagaca gaggagaatg aacgctcgct
     3301 atcaaactca gccagtcaca ctgggagagg tggagcaggt gcagagtgga aagctcattc
     3361 ctttctcacc tgccgtgaac acatcagtgt ctaccgtagc atccacggtt gctccaatgt
     3421 atgccggaga tcttcgcaca aagccacctc ttgaccacaa tgcaagtgcc actgactata
     3481 agttttcttc ttcaatagaa aattcggact ctccagttag aagcattctg aaatcgcaag
     3541 cttggcagcc tttggtagag ggtagcgaga acaagggaat gttgagagaa tatggagaga
     3601 cagaaagcaa gagagctttg acaggtcgag acagtgggat ggagaagtat gggtcctttg
     3661 aggaagcaga agcatcctac cccatcctga accgagccag ggaaggagac agccataagg
     3721 aatctaaata tgctgttccc agaagaggaa gcctggaacg ggcgaaccct cccatcaccc
     3781 acctcgggga tgaaccgaag gaattttcca tggctaaaat gaatgcacaa ggaaacttgg
     3841 acttgaggga caggctgccc tttgaagaga aggtggaggt ggagaatgtt atgaaaagga
     3901 agttttcact aagagcggca gagttcgggg agcccacttc cgagcagacg gggacagctg
     3961 ctgggaaaac tattgctcaa accacagccc ccgtgtcctg gaagccccag gattcttcgg
     4021 aacagccaca ggagaagctc tgcaagaatc catgtgcgat gtttgctgct ggagagatca
     4081 aaacgccgac aggggagggc cttcttgact cacccagcaa aaccatgtct attaaagaaa
     4141 gattggcact gttgaagaaa agcggggagg aagattggag aaacagactc agcaggaggc
     4201 aggagggcgg caaggcgccg gccagcagcc tgcacaccca ggaagcaggg cggtccctca
     4261 tcaagaagcg ggtcacagaa agtcgagaga gccaaatgac gattgaggag aggaagcagc
     4321 tcatcactgt gagagaggag gcctggaaga cgagaggcag aggagcggcc aacgactcga
     4381 cccagttcac tgtggctggc aggatggtga agaaaggttt ggcgtcacct actgccataa
     4441 ccccagtagc ctcacccatt tgcggtaaaa caagaggcac cacacccgtt tccaaacccc
     4501 tggaagatat cgaagccaga ccagatatgc agttagaatc ggacctgaag ttggacaggc
     4561 tggaaacctt tctaagaagg ctgaataaca aagttggcgg gatgcacgaa acggtgctca
     4621 ctgtcaccgg caaatctgtg aaggaggtga tgaagccaga tgatgatgaa acctttgcca
     4681 aattttaccg cagcgtggat tataatatgc caagaagtcc tgtggagatg gatgaggact
     4741 tcgatgtcat tttcgatcct tatgcaccca aattgacgtc ttccgtggcc gagcacaagc
     4801 gggcagttag gcccaagcgc cgggttcagg cctccaaaaa ccccctgaaa atgctggcgg
     4861 caagagaaga tctccttcag gaatacactg agcagagatt aaacgttgcc ttcatggagt
     4921 caaagcggat gaaagtagaa aagatgtctt ccaactccaa cttctcagaa gtcaccctgg
     4981 cgggtttagc cagtaaagaa aacttcagca acgtcagcct gcggagcgtc aacctgacgg
     5041 aacagaactc taacaacagc gccgtgccct acaagaggct gatgctgttg cagattaaag
     5101 gaagaagaca tgtgcagacc aggctggtgg aacctcgagc ttcggcgctc aacagtgggg
     5161 actgcttcct cctgctctct ccccactgct gcttcctgtg ggtaggagag tttgcaaacg
     5221 tcatagaaaa ggcgaaggcc tcagaacttg caactttaat tcagacaaag agggaacttg
     5281 gttgtagagc tacttatatc caaaccattg aagaaggaat taatacacac actcatgcag
     5341 ccaaagactt ctggaagctt ctgggtggcc aaaccagtta ccaatctgct ggagacccaa
     5401 aagaagatga actctatgaa gcagccataa tagaaactaa ctgcatttac cgtctcatgg
     5461 atgacaaact tgttcctgat gacgactact gggggaaaat tccgaagtgc tcccttctgc
     5521 aacccaaaga ggtactggtg tttgattttg gtagtgaagt ttacgtatgg catgggaaag
     5581 aagtcacatt agcacaacga aaaatagcat ttcagctggc aaagcactta tggaatggaa
     5641 cctttgacta tgagaactgt gacatcaatc ccctggatcc tggagaatgc aatccgctta
     5701 tccccagaaa aggacagggg cggcccgact gggcgatatt tgggagactt actgaacaca
     5761 atgagacgat tttgttcaaa gagaagtttc tggattggac ggaactgaag agatcgaatg
     5821 agaagaaccc cggggaactt gcccagcaca aggaagaccc caggactgat gtcaaggcat
     5881 acgatgtgac acggatggtg tccatgcccc agacgacagc aggcaccatc ctggacggag
     5941 tgaacgtcgg ccgtggctat ggcctggtgg aaggacacga caggaggcag tttgagatca
     6001 ccagcgtttc cgtggatgtc tggcacatcc tggaattcga ctatagcagg ctccccaaac
     6061 aaagcatcgg gcagttccat gagggggatg cctatgtggt caagtggaag ttcatggtga
     6121 gcacggcagt gggaagtcgc cagaagggag agcactcggt gagggcagcc ggcaaagaga
     6181 agtgcgtcta cttcttctgg caaggccggc actccaccgt gagtgagaag ggcacgtcgg
     6241 cgctgatgac ggtggagctg gacgaggaaa ggggggccca ggtccaggtt ctccagggaa
     6301 aggagccccc ctgtttcctg cagtgtttcc agggggggat ggtggtgcac tcggggaggc
     6361 gggaagagga agaagaaaat gtgcaaagtg agtggcggct gtactgcgtg cgtggagagg
     6421 tgcccgtgga agggaatttg ctggaagtgg cctgtcactg tagcagcctg aggtccagaa
     6481 cttccatggt ggtgcttaac gtcaacaagg ccctcatcta cctgtggcac ggatgcaaag
     6541 cccaggccca cacgaaggag gtcggaagga ccgctgcgaa caagatcaag gaacaatgtc
     6601 ccctggaagc aggactgcat agtagcagca aagtcacaat acacgagtgt gatgaaggct
     6661 ccgagccact cggattctgg gatgccttag gaaggagaga caggaaagcc tacgattgca
     6721 tgcttcaaga tcctggaagt tttaacttcg cgccccgcct gttcatcctc agcagctcct
     6781 ctggggattt tgcagccaca gagtttgtgt accctgcccg agccccctct gtggtcagtt
     6841 ccatgccctt cctgcaggaa gatctgtaca gcgcgcccca gccagcactt ttccttgttg
     6901 acaatcacca cgaggtgtac ctctggcaag gctggtggcc catcgagaac aagatcactg
     6961 gttccgcccg catccgctgg gcctccgacc ggaagagtgc gatggagact gtgctccagt
     7021 actgcaaagg aaaaaatctc aagaaaccag cccccaagtc ttaccttatc cacgctggtc
     7081 tggagcccct gacattcacc aatatgtttc ccagctggga gcacagagag gacatcgctg
     7141 agatcacaga gatggacacg gaagtttcca atcagatcac cctcgtggaa gacgtcttag
     7201 ccaagctctg taaaaccatt tacccgctgg ccgacctcct ggccaggcca ctcccggagg
     7261 gggtcgatcc tctgaagctt gagatctatc tcaccgacga agacttcgag tttgcactag
     7321 acatgacgag ggatgaatac aacgccctgc ccgcctggaa gcaggtgaac ctgaagaaag
     7381 caaaaggcct gttctgagtg gggagacgcc agaggagcct cacggtcacg tccaacaaca
     7441 ccactgcacc agggaaatgg atatatattt ttggactggt gtttttcaca aagtattttt
     7501 caatcagagt tttcagaacc tgacattgtt aaagatactg cttgtcccgg agttgtgtat
     7561 tttgtaaatg ttcaagggaa ctgtttggaa acttctttcc accattcagg aggttatcag
     7621 aattaataaa agtatctgtt atgtgcactt aagccgcagc tgctatagat agcactgcct
     7681 tcttgttcca gctaggcaat gccttttttt tttttttttg aagcagttct ctttataaag
     7741 tgttattttg atagtttgtg gattctaaaa tatatatata tttatataaa caccatataa
     7801 gtcaaatatg tatttaacaa agcaatatgt attcattcac tttcaagatt tgttttggtg
     7861 tcaaaataac atgaaaaggt agatggagtt gcttctgttg aattagctct gccaccaata
     7921 tgtatcttca tacacgtttg gaaatgtttc ctgcagcatt aggtatgact tgttctgagt
     7981 actgcttccg gtgctaaaat gaacaaagaa tttgtactta atggcatgga ctctggagaa
     8041 tctatgcgaa tcaacctttc taccttaata tctccccaaa aatgtatagt gccttgtttt
     8101 tatgtacagt ttatatacag aaaagtttgc tctgcatttt tgatgatggt ttggaacatt
     8161 atctacaatt ttactctcaa atagtcaaaa taaaaacatc tcaatttcta ataccggttg
     8221 taaacagtac acatgtcatt ttgtgatata ggactcccaa ataaaagtat cagaataaac
     8281 acaacaatta actggttcac attgaa
//
