LOCUS       NM_001128205            5662 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens sulfatase 1 (SULF1), transcript variant 1, mRNA.
ACCESSION   NM_001128205
VERSION     NM_001128205.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Lin,Z.S., Chung,C.C., Liu,Y.C., Chang,C.H., Liu,H.C., Liang,Y.Y.,
            Huang,T.L., Chen,T.M., Lee,C.H., Tang,C.H., Hung,M.C. and Chen,Y.H.
  TITLE     EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to
            shape cartilage tumor progression
  JOURNAL   Elife 12, e79432 (2023)
   PUBMED   36622753
  REMARK    GeneRIF: EZH2/hSULF1 axis mediates receptor tyrosine kinase
            signaling to shape cartilage tumor progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Zeng,F., Liu,Y., Ouyang,Q., Sun,Z., Zhang,K., Li,X. and Liu,Y.
  TITLE     Rs3802278 in 3'-UTR of SULF1 associated with platinum resistance
            and survival in Chinese epithelial ovarian cancer patients
  JOURNAL   J Chemother 33 (8), 564-569 (2021)
   PUBMED   34029511
  REMARK    GeneRIF: Rs3802278 in 3'-UTR of SULF1 associated with platinum
            resistance and survival in Chinese epithelial ovarian cancer
            patients.
REFERENCE   3  (bases 1 to 5662)
  AUTHORS   Chen,F., Zhang,Z., Yu,Y., Liu,Q. and Pu,F.
  TITLE     HSulf-1 and palbociclib exert synergistic antitumor effects on
            RB-positive triple-negative breast cancer
  JOURNAL   Int J Oncol 57 (1), 223-236 (2020)
   PUBMED   32377705
  REMARK    GeneRIF: HSulf1 and palbociclib exert synergistic antitumor effects
            on RBpositive triplenegative breast cancer.
REFERENCE   4  (bases 1 to 5662)
  AUTHORS   Chen,L.M., Niu,Y.D., Xiao,M., Li,X.J. and Lin,H.
  TITLE     LncRNA NEAT1 regulated cell proliferation, invasion, migration and
            apoptosis by targeting has-miR-376b-3p/SULF1 axis in non-small cell
            lung cancer
  JOURNAL   Eur Rev Med Pharmacol Sci 24 (9), 4810-4821 (2020)
   PUBMED   32432744
  REMARK    GeneRIF: LncRNA NEAT1 regulated cell proliferation, invasion,
            migration and apoptosis by targeting has-miR-376b-3p/SULF1 axis in
            non-small cell lung cancer.
REFERENCE   5  (bases 1 to 5662)
  AUTHORS   Matusiewicz,M., Rachwalik,M., Krzystek-Korpacka,M., Bielicki,G.,
            Berdowska,I., Nowicki,R., Gamian,A. and Jasinski,M.
  TITLE     Upregulated sulfatase and downregulated MMP-3 in thoracic aortic
            aneurysm
  JOURNAL   Adv Clin Exp Med 29 (5), 565-572 (2020)
   PUBMED   32421262
  REMARK    GeneRIF: Upregulated sulfatase and downregulated MMP-3 in thoracic
            aortic aneurysm.
REFERENCE   6  (bases 1 to 5662)
  AUTHORS   Li,J., Kleeff,J., Abiatari,I., Kayed,H., Giese,N.A., Felix,K.,
            Giese,T., Buchler,M.W. and Friess,H.
  TITLE     Enhanced levels of Hsulf-1 interfere with heparin-binding growth
            factor signaling in pancreatic cancer
  JOURNAL   Mol Cancer 4 (1), 14 (2005)
   PUBMED   15817123
  REMARK    GeneRIF: High expression of Hsulf-1 occurs in the stromal elements
            as well as in the tumor cells in pancreatic cancer and interferes
            with heparin-binding growth factor signaling
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5662)
  AUTHORS   Lai,J.P., Chien,J., Strome,S.E., Staub,J., Montoya,D.P.,
            Greene,E.L., Smith,D.I., Roberts,L.R. and Shridhar,V.
  TITLE     HSulf-1 modulates HGF-mediated tumor cell invasion and signaling in
            head and neck squamous carcinoma
  JOURNAL   Oncogene 23 (7), 1439-1447 (2004)
   PUBMED   14973553
REFERENCE   8  (bases 1 to 5662)
  AUTHORS   Lai,J.P., Chien,J.R., Moser,D.R., Staub,J.K., Aderca,I.,
            Montoya,D.P., Matthews,T.A., Nagorney,D.M., Cunningham,J.M.,
            Smith,D.I., Greene,E.L., Shridhar,V. and Roberts,L.R.
  TITLE     hSulf1 Sulfatase promotes apoptosis of hepatocellular cancer cells
            by decreasing heparin-binding growth factor signaling
  JOURNAL   Gastroenterology 126 (1), 231-248 (2004)
   PUBMED   14699503
  REMARK    GeneRIF: Down-regulation of hSulf1 contributes to
            hepatocarcinogenesis by enhancing heparin-binding growth factor
            signaling and resistance to apoptosis.
REFERENCE   9  (bases 1 to 5662)
  AUTHORS   Lai,J., Chien,J., Staub,J., Avula,R., Greene,E.L., Matthews,T.A.,
            Smith,D.I., Kaufmann,S.H., Roberts,L.R. and Shridhar,V.
  TITLE     Loss of HSulf-1 up-regulates heparin-binding growth factor
            signaling in cancer
  JOURNAL   J Biol Chem 278 (25), 23107-23117 (2003)
   PUBMED   12686563
  REMARK    GeneRIF: modulates signaling by heparin-binding growth factors, and
            HSulf-1 down-regulation represents a novel mechanism by which
            cancer cells can enhance growth factor signaling
REFERENCE   10 (bases 1 to 5662)
  AUTHORS   Morimoto-Tomita,M., Uchimura,K., Werb,Z., Hemmerich,S. and
            Rosen,S.D.
  TITLE     Cloning and characterization of two extracellular heparin-degrading
            endosulfatases in mice and humans
  JOURNAL   J Biol Chem 277 (51), 49175-49185 (2002)
   PUBMED   12368295
  REMARK    GeneRIF: Sulfs are extracellular endosulfatases with strong
            potential for modulating the interactions of heparan sulfate
            proteoglycans in the extracellular microenvironment
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA234578.1, AY101175.1 and
            AF545571.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001128205.1.
            
            Summary: This gene encodes an extracellular heparan sulfate
            endosulfatase. The encoded enzyme selectively removes 6-O-sulfate
            groups from heparan sulfate chains of heparan sulfate proteoglycans
            (HSPGs). The enzyme is secreted through the Golgi and is
            subsequently localized to the cell surface. The expression of this
            gene may be down-regulated in several types of cancer, including
            hepatocellular (HCC), ovarian and breast cancers. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, 3, and 4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.219029.1, BX571746.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000402687.9/ ENSP00000385704.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-492               DA234578.1         89-580
            493-3827            AY101175.1         332-3666
            3828-5662           AF545571.1         3865-5699
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.2-q13.3"
     gene            1..5662
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /note="sulfatase 1"
                     /db_xref="GeneID:23213"
                     /db_xref="HGNC:HGNC:20391"
                     /db_xref="MIM:610012"
     exon            1..279
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            280..441
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            442..536
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..609
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            610..841
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             670..3285
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /EC_number="3.1.6.1"
                     /EC_number="3.1.6.14"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sulfatase FP; extracellular sulfatase Sulf-1;
                     arylsulfatase; N-acetylglucosamine-6-sulfatase"
                     /codon_start=1
                     /product="extracellular sulfatase Sulf-1 isoform 1
                     precursor"
                     /protein_id="NP_001121677.1"
                     /db_xref="CCDS:CCDS6204.1"
                     /db_xref="GeneID:23213"
                     /db_xref="HGNC:HGNC:20391"
                     /db_xref="MIM:610012"
                     /translation="MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPN
                     IILVLTDDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHN
                     HNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWL
                     GLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMV
                     ISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTN
                     ILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPY
                     DFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPE
                     KPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY
                     QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRE
                     SGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEEELQ
                     VLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPTTVRVTHKCFILPND
                     SIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKRRKPEECSCSKQSY
                     YNKEKGVKKQEKLKSHLHPFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECS
                     LPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFL
                     EYFDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCNPRPKNLDVGNKDGG
                     SYDLHRGQLWDGWEG"
     exon            842..1081
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1233
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1403
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1554
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1730
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1859
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1916
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2046
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2263
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2519
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2616
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2711
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2712..2777
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2778..2953
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2954..3096
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3220
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3254
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..5662
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4787..4792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /note="hexamer: AATAAA"
     regulatory      4795..4800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /note="hexamer: AGTAAA"
     polyA_site      4805
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /note="major polyA site"
     polyA_site      4809
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
     regulatory      5642..5647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /note="hexamer: AATAAA"
     polyA_site      5662
                     /gene="SULF1"
                     /gene_synonym="SULF-1"
                     /note="major polyA site"
ORIGIN      
        1 gcagaccgtc gctaatgaat cttggggccg gtgtcgggcc ggggcggctt gatcggcaac
       61 taggaaaccc caggcgcaga ggccaggagc gagggcagcg aggatcagag gccaggcctt
      121 cccggctgcc ggcgctcctc ggaggtcagg gcagatgagg aacatgactc tcccccttcg
      181 gaggaggaag gaagtcccgc tgccacctta tctctgctcc tctgcctcct ccctgttccc
      241 agagcttttt ctctagagaa gattttgaag gcggcttttg gattcttcac ttctcttgaa
      301 caaggaactc actcagagac taacacaaag gaagtaattt cttacctggt cattatttag
      361 tctacaataa gttcatcctt cttcagtgtg accagtaaat tcttcccata ctcttgaaga
      421 gagcataatt ggaatggaga ggtgctgacg gccacccacc atcatctaaa gaagataaac
      481 ttggcaaatg acatgcaggt tcttcaaggc agaataattg cagaaaatct tcaaaggacc
      541 ctatctgcag atgttctgaa tacctctgag aatagagatt gattattcaa ccaggatacc
      601 taattcaaga actccagaaa tcaggagacg gagacatttt gtcagttttg caacattgga
      661 ccaaatacaa tgaagtattc ttgctgtgct ctggttttgg ctgtcctggg cacagaattg
      721 ctgggaagcc tctgttcgac tgtcagatcc ccgaggttca gaggacggat acagcaggaa
      781 cgaaaaaaca tccgacccaa cattattctt gtgcttaccg atgatcaaga tgtggagctg
      841 gggtccctgc aagtcatgaa caaaacgaga aagattatgg aacatggggg ggccaccttc
      901 atcaatgcct ttgtgactac acccatgtgc tgcccgtcac ggtcctccat gctcaccggg
      961 aagtatgtgc acaatcacaa tgtctacacc aacaacgaga actgctcttc cccctcgtgg
     1021 caggccatgc atgagcctcg gacttttgct gtatatctta acaacactgg ctacagaaca
     1081 gccttttttg gaaaatacct caatgaatat aatggcagct acatcccccc tgggtggcga
     1141 gaatggcttg gattaatcaa gaattctcgc ttctataatt acactgtttg tcgcaatggc
     1201 atcaaagaaa agcatggatt tgattatgca aaggactact tcacagactt aatcactaac
     1261 gagagcatta attacttcaa aatgtctaag agaatgtatc cccataggcc cgttatgatg
     1321 gtgatcagcc acgctgcgcc ccacggcccc gaggactcag ccccacagtt ttctaaactg
     1381 taccccaatg cttcccaaca cataactcct agttataact atgcaccaaa tatggataaa
     1441 cactggatta tgcagtacac aggaccaatg ctgcccatcc acatggaatt tacaaacatt
     1501 ctacagcgca aaaggctcca gactttgatg tcagtggatg attctgtgga gaggctgtat
     1561 aacatgctcg tggagacggg ggagctggag aatacttaca tcatttacac cgccgaccat
     1621 ggttaccata ttgggcagtt tggactggtc aaggggaaat ccatgccata tgactttgat
     1681 attcgtgtgc ctttttttat tcgtggtcca agtgtagaac caggatcaat agtcccacag
     1741 atcgttctca acattgactt ggcccccacg atcctggata ttgctgggct cgacacacct
     1801 cctgatgtgg acggcaagtc tgtcctcaaa cttctggacc cagaaaagcc aggtaacagg
     1861 tttcgaacaa acaagaaggc caaaatttgg cgtgatacat tcctagtgga aagaggcaaa
     1921 tttctacgta agaaggaaga atccagcaag aatatccaac agtcaaatca cttgcccaaa
     1981 tatgaacggg tcaaagaact atgccagcag gccaggtacc agacagcctg tgaacaaccg
     2041 gggcagaagt ggcaatgcat tgaggataca tctggcaagc ttcgaattca caagtgtaaa
     2101 ggacccagtg acctgctcac agtccggcag agcacgcgga acctctacgc tcgcggcttc
     2161 catgacaaag acaaagagtg cagttgtagg gagtctggtt accgtgccag cagaagccaa
     2221 agaaagagtc aacggcaatt cttgagaaac caggggactc caaagtacaa gcccagattt
     2281 gtccatactc ggcagacacg ttccttgtcc gtcgaatttg aaggtgaaat atatgacata
     2341 aatctggaag aagaagaaga attgcaagtg ttgcaaccaa gaaacattgc taagcgtcat
     2401 gatgaaggcc acaaggggcc aagagatctc caggcttcca gtggtggcaa caggggcagg
     2461 atgctggcag atagcagcaa cgccgtgggc ccacctacca ctgtccgagt gacacacaag
     2521 tgttttattc ttcccaatga ctctatccat tgtgagagag aactgtacca atcggccaga
     2581 gcgtggaagg accataaggc atacattgac aaagagattg aagctctgca agataaaatt
     2641 aagaatttaa gagaagtgag aggacatctg aagagaagga agcctgagga atgtagctgc
     2701 agtaaacaaa gctattacaa taaagagaaa ggtgtaaaaa agcaagagaa attaaagagc
     2761 catcttcacc cattcaagga ggctgctcag gaagtagata gcaaactgca acttttcaag
     2821 gagaacaacc gtaggaggaa gaaggagagg aaggagaaga gacggcagag gaagggggaa
     2881 gagtgcagcc tgcctggcct cacttgcttc acgcatgaca acaaccactg gcagacagcc
     2941 ccgttctgga acctgggatc tttctgtgct tgcacgagtt ctaacaataa cacctactgg
     3001 tgtttgcgta cagttaatga gacgcataat tttcttttct gtgagtttgc tactggcttt
     3061 ttggagtatt ttgatatgaa tacagatcct tatcagctca caaatacagt gcacacggta
     3121 gaacgaggca ttttgaatca gctacacgta caactaatgg agctcagaag ctgtcaagga
     3181 tataagcagt gcaacccaag acctaagaat cttgatgttg gaaataaaga tggaggaagc
     3241 tatgacctac acagaggaca gttatgggat ggatgggaag gttaatcagc cccgtctcac
     3301 tgcagacatc aactggcaag gcctagagga gctacacagt gtgaatgaaa acatctatga
     3361 gtacagacaa aactacagac ttagtctggt ggactggact aattacttga aggatttaga
     3421 tagagtattt gcactgctga agagtcacta tgagcaaaat aaaacaaata agactcaaac
     3481 tgctcaaagt gacgggttct tggttgtctc tgctgagcac gctgtgtcaa tggagatggc
     3541 ctctgctgac tcagatgaag acccaaggca taaggttggg aaaacacctc atttgacctt
     3601 gccagctgac cttcaaaccc tgcatttgaa ccgaccaaca ttaagtccag agagtaaact
     3661 tgaatggaat aacgacattc cagaagttaa tcatttgaat tctgaacact ggagaaaaac
     3721 cgaaaaatgg acggggcatg aagagactaa tcatctggaa accgatttca gtggcgatgg
     3781 catgacagag ctagagctcg ggcccagccc caggctgcag cccattcgca ggcacccgaa
     3841 agaacttccc cagtatggtg gtcctggaaa ggacattttt gaagatcaac tatatcttcc
     3901 tgtgcattcc gatggaattt cagttcatca gatgttcacc atggccaccg cagaacaccg
     3961 aagtaattcc agcatagcgg ggaagatgtt gaccaaggtg gagaagaatc acgaaaagga
     4021 gaagtcacag cacctagaag gcagcgcctc ctcttcactc tcctctgatt agatgaaact
     4081 gttaccttac cctaaacaca gtatttcttt ttaacttttt tatttgtaaa ctaataaagg
     4141 taatcacagc caccaacatt ccaagctacc ctgggtacct ttgtgcagta gaagctagtg
     4201 agcatgtgag caagcggtgt gcacacggag actcatcgtt ataatttact atctgccaag
     4261 agtagaaaga aaggctgggg atatttgggt tggcttggtt ttgatttttt gcttgtttgt
     4321 ttgttttgta ctaaaacagt attatctttt gaatatcgta gggacataag tatatacatg
     4381 ttatccaatc aagatggcta gaatggtgcc tttctgagtg tctaaaactt gacacccctg
     4441 gtaaatcttt caacacactt ccactgcctg cgtaatgaag ttttgattca tttttaacca
     4501 ctggaatttt tcaatgccgt cattttcagt tagatgattt tgcactttga gattaaaatg
     4561 ccatgtctat ttgattagtc ttattttttt atttttacag gcttatcagt ctcactgttg
     4621 gctgtcattg tgacaaagtc aaataaaccc ccaaggacga cacacagtat ggatcacata
     4681 ttgtttgaca ttaagctttt gccagaaaat gttgcatgtg ttttacctcg acttgctaaa
     4741 atcgattagc agaaaggcat ggctaataat gttggtggtg aaaataaata aataagtaaa
     4801 caaaatgaag attgcctgct ctctctgtgc ctagcctcaa agcgttcatc atacatcata
     4861 cctttaagat tgctatattt tgggttattt tcttgacagg agaaaaagat ctaaagatct
     4921 tttattttca tcttttttgg ttttcttggc atgactaaga agcttaaatg ttgataaaat
     4981 atgactagtt ttgaatttac accaagaact tctcaataaa agaaaatcat gaatgctcca
     5041 caatttcaac ataccacaag agaagttaat ttcttaacat tgtgttctat gattatttgt
     5101 aagaccttca ccaagttctg atatctttta aagacatagt tcaaaattgc ttttgaaaat
     5161 ctgtattctt gaaaatatcc ttgttgtgta ttaggttttt aaataccagc taaaggatta
     5221 cctcactgag tcatcagtac cctcctattc agctccccaa gatgatgtgt ttttgcttac
     5281 cctaagagag gttttcttct tatttttaga taattcaagt gcttagataa attatgtttt
     5341 ctttaagtgt ttatggtaaa ctcttttaaa gaaaatttaa tatgttatag ctgaatcttt
     5401 ttggtaactt taaatcttta tcatagactc tgtacatatg ttcaaattag ctgcttgcct
     5461 gatgtgtgta tcatcggtgg gatgacagaa caaacatatt tatgatcatg aataatgtgc
     5521 tttgtaaaaa gatttcaagt tattaggaag catactctgt tttttaatca tgtataatat
     5581 tccatgatac ttttatagaa caattctggc ttcaggaaag tctagaagca atatttcttc
     5641 aaataaaagg tgtttaaact tt
//
