LOCUS       NM_006704              14161 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly
            cochaperone (SUGT1), transcript variant 2, mRNA.
ACCESSION   NM_006704
VERSION     NM_006704.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Bohush,A., Goral,A., Sierant,M., Nawrot,B., Lesniak,W. and
            Filipek,A.
  TITLE     Sgt1 Regulates alpha-Synuclein Subcellular Localization and
            Expression of Parkinson's Disease Related Genes, PINK1 and PARK9
  JOURNAL   Biomolecules 11 (11), 1675 (2021)
   PUBMED   34827672
  REMARK    GeneRIF: Sgt1 Regulates alpha-Synuclein Subcellular Localization
            and Expression of Parkinson's Disease Related Genes, PINK1 and
            PARK9.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  AUTHORS   Allouch,A., Di Primio,C., Paoletti,A., Le-Bury,G., Subra,F.,
            Quercioli,V., Nardacci,R., David,A., Saidi,H., Cereseto,A.,
            Ojcius,D.M., Montagnac,G., Niedergang,F., Pancino,G.,
            Saez-Cirion,A., Piacentini,M., Gougeon,M.L., Kroemer,G. and
            Perfettini,J.L.
  TITLE     SUGT1 controls susceptibility to HIV-1 infection by stabilizing
            microtubule plus-ends
  JOURNAL   Cell Death Differ 27 (12), 3243-3257 (2020)
   PUBMED   32514048
  REMARK    GeneRIF: SUGT1 controls susceptibility to HIV-1 infection by
            stabilizing microtubule plus-ends.
REFERENCE   3  (bases 1 to 14161)
  AUTHORS   Filipek,A. and Lesniak,W.
  TITLE     Current view on cellular function of S100A6 and its ligands,
            CacyBP/SIP and Sgt1
  JOURNAL   Postepy Biochem 64 (3), 242-252 (2018)
   PUBMED   30656909
  REMARK    GeneRIF: REVIEW: cellular function of S100A6 and its ligands,
            CacyBP/SIP and Sgt1
            Review article
REFERENCE   4  (bases 1 to 14161)
  AUTHORS   Niikura,Y., Kitagawa,R., Ogi,H. and Kitagawa,K.
  TITLE     SGT1-HSP90 complex is required for CENP-A deposition at centromeres
  JOURNAL   Cell Cycle 16 (18), 1683-1694 (2017)
   PUBMED   28816574
  REMARK    GeneRIF: the SGT1-HSP90 complex contributes to the E3 ligase
            activity of the CUL4A complex that is necessary for CENP-A
            ubiquitylation and CENP-A deposition at the centromere.
REFERENCE   5  (bases 1 to 14161)
  AUTHORS   Steensgaard,P., Garre,M., Muradore,I., Transidico,P., Nigg,E.A.,
            Kitagawa,K., Earnshaw,W.C., Faretta,M. and Musacchio,A.
  TITLE     Sgt1 is required for human kinetochore assembly
  JOURNAL   EMBO Rep 5 (6), 626-631 (2004)
   PUBMED   15133482
REFERENCE   6  (bases 1 to 14161)
  AUTHORS   Lee,Y.T., Jacob,J., Michowski,W., Nowotny,M., Kuznicki,J. and
            Chazin,W.J.
  TITLE     Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the
            tetratricopeptide repeat domain
  JOURNAL   J Biol Chem 279 (16), 16511-16517 (2004)
   PUBMED   14761955
REFERENCE   7  (bases 1 to 14161)
  AUTHORS   Zou,X., Ji,C., Wang,L., Wu,M., Zheng,H., Xu,J., Jin,F., Gu,S.,
            Ying,K., Xie,Y. and Mao,Y.
  TITLE     Molecular cloning and characterization of SGT1.2, a novel splice
            variant of Homo sapiens SGT1
  JOURNAL   DNA Seq 15 (2), 140-143 (2004)
   PUBMED   15346769
  REMARK    GeneRIF: SGt1.2 is a splicing variant of SGT1 and widely expressed
            in human adult tissue.
REFERENCE   8  (bases 1 to 14161)
  AUTHORS   Niikura,Y. and Kitagawa,K.
  TITLE     Identification of a novel splice variant: human SGT1B (SUGT1B)
  JOURNAL   DNA Seq 14 (6), 436-441 (2003)
   PUBMED   15018354
REFERENCE   9  (bases 1 to 14161)
  AUTHORS   Nowotny,M., Spiechowicz,M., Jastrzebska,B., Filipek,A., Kitagawa,K.
            and Kuznicki,J.
  TITLE     Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and
            other S100 proteins
  JOURNAL   J Biol Chem 278 (29), 26923-26928 (2003)
   PUBMED   12746458
  REMARK    GeneRIF: Sgt1 binds to S100A6 in a calcium-regulated manner
REFERENCE   10 (bases 1 to 14161)
  AUTHORS   Kitagawa,K., Skowyra,D., Elledge,S.J., Harper,J.W. and Hieter,P.
  TITLE     SGT1 encodes an essential component of the yeast kinetochore
            assembly pathway and a novel subunit of the SCF ubiquitin ligase
            complex
  JOURNAL   Mol Cell 4 (1), 21-33 (1999)
   PUBMED   10445024
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139089.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_006704.4.
            
            Summary: This gene encodes a highly conserved nuclear protein
            involved in kinetochore function and required for the G1/S and G2/M
            transitions. This protein interacts with heat shock protein 90.
            Alternative splicing results in multiple transcript variants.
            Pseudogenes for this gene have been defined on several different
            chromosomes. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (2, also known as A) lacks an
            alternate in-frame exon compared to variant 1. The resulting
            isoform (A) is shorter than isoform B.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.91720.1,
                                           SRR1803614.129272.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310528.9/ ENSP00000308067.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AL139089.13        8419-8541
            124-181             AL139089.13        8629-8686
            182-272             AL139089.13        13115-13205
            273-342             AL139089.13        13982-14051
            343-413             AL139089.13        14762-14832
            414-467             AL139089.13        18232-18285
            468-484             AL139089.13        18679-18695
            485-507             AL139089.13        19618-19640
            508-604             AL139089.13        21220-21316
            605-712             AL139089.13        22395-22502
            713-803             AL139089.13        31813-31903
            804-985             AL139089.13        35557-35738
            986-14161           AL139089.13        43317-56492
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.3"
     gene            1..14161
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="SGT1 homolog, MIS12 kinetochore complex assembly
                     cochaperone"
                     /db_xref="GeneID:10910"
                     /db_xref="HGNC:HGNC:16987"
                     /db_xref="MIM:604098"
     exon            1..123
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1087
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="isoform A is encoded by transcript variant 2;
                     suppressor of G2 allele of SKP1, S. cerevisiae, homolog
                     of; putative 40-6-3 protein; protein SGT1 homolog; SGT1,
                     suppressor of G2 allele of SKP1"
                     /codon_start=1
                     /product="protein SGT1 homolog isoform A"
                     /protein_id="NP_006695.1"
                     /db_xref="CCDS:CCDS9436.1"
                     /db_xref="GeneID:10910"
                     /db_xref="HGNC:HGNC:16987"
                     /db_xref="MIM:604098"
                     /translation="MAAAAAGTATSQRFFQSFSDALIDEDPQAALEELTKALEQKPDD
                     AQYYCQRAYCHILLGNYCVAVADAKKSLELNPNNSTAMLRKGICEYHEKNYAAALETF
                     TEGQKLDSADANFSVWIKRCQEAQNGSESEVWTHQSKIKYDWYQTESQVVITLMIKNV
                     QKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPE
                     AVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAA
                     LNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY"
     exon            124..181
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            182..272
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            273..342
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            343..413
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..467
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            468..484
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            485..507
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            508..604
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            605..712
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            713..803
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            804..985
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            986..14161
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1087..1092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATAAA"
     polyA_site      1107
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="major polyA site"
     regulatory      1527..1532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATACA"
     polyA_site      1545
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="major polyA site"
     regulatory      13984..13989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATAAA"
     polyA_site      14006
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
     regulatory      14141..14146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATAAA"
     polyA_site      14161
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
ORIGIN      
        1 ctccagaagt ttcccccttg ggcggtggtg gaggtggtaa ccgtgatagt agcagctccg
       61 gcggcagcaa cagcgactac gagggatggc ggcggctgca gcaggaactg caacatccca
      121 gaggtttttc cagagcttct cggatgccct aatcgacgag gacccccagg cggcgttaga
      181 ggagctgact aaggctttgg aacagaaacc agatgatgca cagtattatt gtcaaagagc
      241 ttattgtcac attcttcttg ggaattactg tgttgctgtt gctgatgcaa agaagtctct
      301 agaactcaat ccaaataatt ccactgctat gctgagaaaa ggaatatgtg aataccatga
      361 aaaaaactat gctgctgccc tagaaacttt tacagaagga caaaaattag atagtgcaga
      421 tgctaatttc agtgtctgga ttaaaaggtg tcaagaagct cagaatggct cagaatctga
      481 ggtgtggact catcagtcaa aaatcaagta tgactggtat caaacagaat ctcaagtagt
      541 cattacactt atgatcaaga atgttcagaa gaatgatgta aatgtggaat tttcagaaaa
      601 agagttgtct gctttggtta aacttccttc tggagaggat tacaatttga aactggaact
      661 tcttcatcct ataataccag aacagagcac gtttaaagta ctttcaacaa agattgaaat
      721 taaactgaaa aagccagagg ctgtgagatg ggaaaagcta gaggggcaag gagatgtgcc
      781 tacgccaaaa caattcgtag cagatgtaaa gaacctatat ccatcatcat ctccttatac
      841 aagaaattgg gataaattgg ttggtgagat caaagaagaa gaaaagaatg aaaagttgga
      901 gggagatgca gctttaaaca gattatttca gcagatctat tcagatggtt ctgatgaagt
      961 gaaacgtgcc atgaacaaat cctttatgga gtcgggtggt acagttttga gtaccaactg
     1021 gtctgatgta ggtaaaagga aagttgaaat caatcctcct gatgatatgg aatggaaaaa
     1081 gtactaaata aattaatttg ctctcatcgt attgtgtata ttcacctaat gcccattgtg
     1141 tattgatatt gcattcttga attttgaaca ctgaatatct ttttgaaaga ttatacttct
     1201 ttacctcttt gtgctttaga aattattttc cttcaagtgt tcaagtctaa tgaagaatga
     1261 agataacatt ttatcacttc tgtccttaaa ggtttcagac atggtgaaac tgaataaagc
     1321 atgtcatttg ctcctagata gattcattct atctagttgt ggggatggag aaatctttaa
     1381 tggtatattt tcggttattg ccttattttt gatacagtat tctgtcagta atttattaga
     1441 cctggcagct ttgggtgagc ttagattttt caccttcagt gttacattgt gtttgctttt
     1501 aaaaactgct tttgaatgga gttgtaaata caatttttct atgaagatgt ttggtttatt
     1561 tacctttttc ttaagttttt aatgagaact ttgagaaatt atcacaggga attcccatgt
     1621 agttctttta atgggaagaa tttgtaaatt taattgcact gagatatttt ggtcgttacc
     1681 ttgcaagttt ctgtataaaa catagatacc tgagttttaa cacactgcaa gtttatacta
     1741 aggataatct gtgaaggtag ggcaaagaag gttctaggaa agcccaactc cacttgcttc
     1801 ttttatcacg acaggattct tgaaattaca gcattggtgg gttttttggt attttttttc
     1861 tttattagaa aatctctgtt gtcacgtccc catgattact ttggtttcat tggtttatgg
     1921 taactgctgt gagagtaaat taatgtgtat atgtttagta tttactctgt gcctagaata
     1981 tatagatatc atgagaatac aaaaaattaa agacatgatt attaacttca aagagcttat
     2041 aaatgtagca gggaaaaaaa ataacattca tgaaggaact gtggaaagat tgaatgctga
     2101 actcggtata aatacacttt agagacagga aaatgttctg tgattaaaat tgtttacagt
     2161 ctaggctgtt ataaagtata gactatggat gacctgtaga tctgactttg ttttctcacc
     2221 atgattttct caactgtaat accccagatg ttggtctcag ctctgttagg tgtcactaac
     2281 atttttatgg caaagcacta ctctataccc tcagagcagg gggagtggga ggcaaacggg
     2341 tcatcatttc agcctccaaa acagtaggaa ttcagctcct tccttctcgt cctttgttgt
     2401 ggggtgtagg ggagttacta aggaaagttt cagtaaggtg ataccagatg gtgcccaaac
     2461 ctgttgttat agcttgttta agcattctag agttttcact tgtccttctg tccacttaga
     2521 gctactagat acattttttt cttcttgaac actgaaggaa aaggcaaaat taagttttgg
     2581 ttggatggat ttctaaagtt ttcttcttag gaaaatagga tagtgacttt tgtattctgt
     2641 atgttaaatt ttctcagtac cattaatcct aagatgaaga ggaaggctat tgctgcagag
     2701 gcatagttga ggacgtatag cactatacca gagacaagaa tgcttagctc tagcaagtct
     2761 tctgtcattg actaccttca taatcttcag caggtttagg cctttcttag ttcctttgta
     2821 aataatccat agagtcttag agttataatt aaaggtccat ggttctaaat acagaagcat
     2881 atttttgccg cttaactttt tctttcttaa agatgacact attaaaatag ctgtttctga
     2941 ccaggtgcag tagctcatgc ttgtaatccc agcattttgg gaggcggagg tgggcagatc
     3001 acctgaggtc aggagttcaa gaccagcctg gccaacatgg tgaaaccctg tgtctactaa
     3061 aaatgcaaaa attagctggg tgtagtggca tgcacctgta gtcccagcta cttgggaggc
     3121 tcaggcaggg agaattgctt gaaccgggag gcagggggtg cagtgagcca agatcatgcc
     3181 actgcactcc agcctgggcg actccatctc aaaaaaaata aatagctgtt taaaaaaaaa
     3241 aaatctggta ctatctttag ttggttttaa aggatatatt atctagaata tgcttaccca
     3301 ttggctagat ttgctaagtg ttgtgggatt tgggattttg ctggtctgaa gggagtttgt
     3361 aaaacacatg actgaaagca ttataaaaca taccttgtca aatacattag attcattata
     3421 ggtcaaggga attttgaaaa gtaggctaga ttgctgccta agaatcccac tctaaagtac
     3481 tgctttgttt aatcattcta gataatggaa gcttgtaagc ctccagaaag ttaaaacaaa
     3541 aaacttcttt ctgattaaga gcctcttaac tctatcctta tcctctgcta tctcaatgta
     3601 gctattaatt gttccctctt tgtttctaga ctccaatttt catttctcaa attttggttc
     3661 tcagtgctgt tagtattagt agccttttgt ttcttcagaa tgtatgtatt tttcctctta
     3721 aatgtttcct ttccctttta aggttagtcc tggaatacat gtttttcttg gtcttgtaga
     3781 cttcatctct tctgatttcc ataataattt gcaccttgat gaatccagaa ttctcccact
     3841 gtcttcaaag aatcttgaat agaataatct gctttttgaa ctctgctatc tagtaatctc
     3901 tttagatatc tataccacaa gtttgaaaca tcaaggtcaa ttttgataat ttcctgtctt
     3961 atgtcttaat acccattcag agttgactgc caactaacaa gctcagcctt tagcactggc
     4021 acttactggc caatctgttt tctgtctata gggagtgaaa tggaatggag aaagaggatg
     4081 gcttgcatta ataactctag tttttaaaaa catttttatt ccttttttaa aaatttgttt
     4141 agagacacgg tcttcttcta tcacctaggc tggagtgcag tggtgcgatc atggctcact
     4201 gtagcctcca actcctaggc tcaagtaatc ctctttcctc ggcttcccaa agtgctggga
     4261 ttacaggtgt gagtcactgt gcctggccca cattttcatt cttggctcac tgtagcctta
     4321 atctttcagg ctcaaatgat tctcccatct cagcctccca agtagctggg accacaggca
     4381 tgcatcacca cgactggcta atttttgtat tttttttgta gaaccgggtt tcaccatgtg
     4441 cccaggctgg tcttgaactt ctgggctcaa gttatctacc tgcctcagcc tacccaaagt
     4501 gctgggatta caggagtgag ccaccatgcc cagttatgaa cttcaagtaa atatttaaaa
     4561 tggagataca tcacaatgct attatgaata atttagatag aaattttaat ttagttcagt
     4621 acttaaatat tttttggctc ttctttccca ctttaccttt ctctattcct tcctcagtta
     4681 tctacatatc agattagatt attgatatct gagatatatc actgacccag agcaaaattg
     4741 catattagtt taccatctaa tcagcctaat gcacttaaat tcccttggaa tctttcctgt
     4801 tcagattggt gtgcatctct ctgaacttgt agctttactg acatttccaa aaaaaggtag
     4861 acggtttcta ggtggtgtgt gtttttgtta gttactagag ccacttaccc tttgtaacta
     4921 gctctaaaga agtacatctt ttgctcatac ctgctactgt aaccatatga cagtatggat
     4981 ttaagttatt tctgcctaga ggaattagat gataaaagct aaaaggacaa aatactggtt
     5041 tctgtgcagg gaagggtcat atgtagtttt aaataaaagg gttcatctct tttgatgtgg
     5101 tttccctttc tccatctgtg cattttttaa atcgtaagat cattttacac acaccaagag
     5161 attttatttt gggaacttgt ggggaaaata ttgatttacc tacaaatgtt actatttgga
     5221 gccatccttg tgattggttt ctgagattaa tttaactctt agcctcactt cctaccttag
     5281 taaagttgct cctccaaatc agctaaaatc atatacaatg aatgctgact cattactgca
     5341 aatagtggta gttactgtgt actagcattt cttatggatt gggtggcatt atctcatttt
     5401 taatcgccct tcaatagcat cccattattg caaatcagaa agatgatggg gccaaagtaa
     5461 agttctttgc ccaggtcact ctactagtaa gtaattgcaa tagctgggat ttgaacccag
     5521 gcctatcaga ctccattacc catacttctg acaactgcaa tcctctcatc aggttaataa
     5581 gctagatgtg gaatgaatga tttttattac caaatcagct gactggaaat gaagcagaga
     5641 atattcaagt taaagaacta attctttttt tttttttttt tgagacagtc tcactctgtc
     5701 gttcagggaa ggaatgcagt ggtgcaatct tggctcactg caacctccac ctcccaggtt
     5761 caagtaattc ttctgcctca gcctcctgag tagctgggat tacaggcatg ggccgccatg
     5821 cccagctaat ttttgtattt ttagtagaga cgaggtttca ccatgttggt caggctggtc
     5881 ttgaactcct gacctcatga tccgcccacc ttggcctccc aaaatgctgg gattataggc
     5941 gtgagccact gtgcccagcc aagaactaat tcttatatag gagctgggaa gataaattcc
     6001 tagagatcct cactttctta agtaggaaag cttattagga atccaaatct tttgcctcat
     6061 actggaagta atgcaaagaa gtagtctgat ttttgagagg gtgctgtttt tctcaaatct
     6121 agatcattac agagaaacag tgtactttta acaactgttg catgtgtaag aagactggta
     6181 catgatgcca atgaaattct ttttttcctt taacttggga gctctcatac cagttccgtt
     6241 gtacaaatgc tgagattgtt tcaaggtttt attcctacag acatgttagg atttgtgtat
     6301 cactgatttc tccaataaaa tttgaatatt tcaggttatt tcctttttcc taacttatgt
     6361 tagcattttg tatatatata tatatttgaa ttttggcttg tctggagtct taaatatttg
     6421 gtatgccttc acatttggat ttttagtttt atttttacat tgagacttct acctatatca
     6481 ttgactttat tttttcctat gattaaagga ttcagttata caaaaagatg tttcagaaac
     6541 actgctattt acctaaaggt aattttcagt ttctttagaa ggagttagga aaatggtcct
     6601 taatacctat atagggtttt ttaaaaaact gtaccctata atgcagccac agaagatcta
     6661 aataataaac aattgcgcat actattaata tatatttaga gcttttgtta agtacatgaa
     6721 aagacagtat tgcatagtgg ttaaatgcat ggactctgaa gccatcctgt ttgggatcat
     6781 cctggctaca ttgcttacta gctgtgtgac aatggatata ggtgcttaat gtctctgtgc
     6841 ctcagttttt gtgtctatgt aaaggagata ataggatccc cctcatgcct catggtctta
     6901 gttgaatgaa ttcatgtaac tccacaggta tcttaatcca gaaggctcaa aatactaatg
     6961 ttttgtgtgt gtgtgtgtgt gtgcatgtgc atctgtatga caagtgatat aaaacgtgac
     7021 ctgaatttat aatatgaata gtcacatttt gcagcagaaa tattaaatgt ttaattatag
     7081 aatgctagca ctgatcctgc tgaaattgtt acaaaataca gcagttgccc cttaactgtg
     7141 gcagatacat tccaagaccc ccagtactga atgctgtatg tacatttttt atacatgtat
     7201 acttatgata aagtttataa attaggcaca gtaagatatt aataatagaa tatacaatat
     7261 accgcaataa aacttatgtg aacagttatt tttggacagc agttgactgt gggtcactga
     7321 aattgtggaa agcaaaacca tggataaagg ggaactatta atagtctatc tgtattgcat
     7381 tatcttctaa aatccaaaat tctgacttca aaaactcatc tgagggtttt ggaattgtgt
     7441 atctatgtgt gtgaagtgca aagagcagtg cttcatgtga cagataacat cagatgatcc
     7501 taggctggaa ggtggaaatc ctagagaaga tttttttgtg ttgtaagttc agtctttcaa
     7561 gatgtggaat tctgtctgag gaaagtatat cacctagtta acaattgtta ctgtaattta
     7621 taaaatttgt actttggaga gcataagaat tcatgaaaag tttttaataa ttttacttaa
     7681 acagagctta attatgtaag tattgtcagc actttataaa gaatcgtaaa ataaaatagt
     7741 ggcagaaaat ataaactaga tgtagaatgt taaggaactt tcggaagttc tctggttttc
     7801 atttcaattt caaagctcaa aacagaaggt aaaactatta ggatttgtga aaaatacaag
     7861 gtacaaaatc acgcagttta taaaccacag attgtcttgt tattcccatc agtgaaaaga
     7921 agctgatatt tgtttgtgtt tgtttttgtt tttgtttttg agacggagtc ttgctctgtc
     7981 gcccaggctg gagtgcagtg gcgccatttt ggctcactgc aagctccgcc tcccggttca
     8041 cgccattctc atgcctcagc ctcccaagta gctgggacta caggcgtccg ccaccatgcc
     8101 cggctaattt tttgtatttt tagtagagac aagagtttca ccgtgttagc caggatggtc
     8161 tcatctcctg atcttgtgac ccgcccgcct cggcctccca aagtgctggg attacaggcg
     8221 tgagccacca tgcccagcct gctggtagat gtttttatga gttaattcat caatcttatt
     8281 attcttgccc caaatgaatc agtgtggagc atatacaagt gtgtagacat taatatgggc
     8341 tcactgtcag ctgggagttc tcacaactac cactttttac tgattatctg ttttctaaac
     8401 tttttctgtg gcttgaagtg actgaaagtg aacacattca taagtaatga gcttaggctg
     8461 aggatatata tccagtgggg gatgaaacat gcttaagaaa ttgagtgcat ttcctttatt
     8521 attgttatta ttttgctatt tcaaaacaat tgactttaaa aaattatact tcaccacaga
     8581 gacaggtgtt gttttgtaat ggaaggagta gtaacttatg aatctagact cttaaaatcc
     8641 cagttctgat actttcataa ttgtgtgttt tgagggcaag tgacttatct gaactttttt
     8701 cttttttata acactggatg ataattaacc ctcaggattg tgaggattta ataactaagt
     8761 cctgactcaa attatgttac caataaatgc tacttaaatg taaagtcctt gatttatttt
     8821 ttcccctcag tcctgtttag tgttgaaatt cttcaaagga gggtgagtct ggcatttacc
     8881 tgctattcaa gagttttcag aattcaaccc cagccaaaat ttttggccac atctcttgtc
     8941 actgctttgc tgtgtactaa agctcccaga cacacaagaa tcttctagtc atggtcaaac
     9001 tttttctgca aagggcactt acagtctatt gcatattctt tgtttttagt gttcagacat
     9061 ctgctgtttc tgaatatgtc gtgtattttc attccttttt ctgcccctcc tgggcatctc
     9121 cagtttccta ctgatgtgtc aaaactgagt ttgtatgcta cctccaggaa agcagtctaa
     9181 tcttttgcaa ctctgttact gacttttgtg gcattggatt gggttatttg cttatggttg
     9241 agacatcact ggatcctttg atggagtcat accaaaacca caacatgtca tgagcatgct
     9301 tgagagtgct aatctattcc ttgtttgtca gaccagtaca ttacagttgg ttcatttctt
     9361 cccccgtgtg actgagtttc ttgagggcgc ggtctagctt ccatctttgt atgtgtagca
     9421 cctggtacat agagaacagg ctcccagaat tggatggatt tgctcatggt cttatcactg
     9481 cacagaatgc ccttattttc ttccattcat tctatgagat ctcaattaaa atgtcagtac
     9541 tcttgtggaa ctcccctctc catgccctct actgaaatgc attgccactc tagttgtgat
     9601 gtgggagtgc cttgtttatg cctctattta tgacaccttc atggtgtcac atgttatagg
     9661 gttagttgcg gctgtgtctg ttttaccttg tgtgtcttgg tcagttagtg ttatttactt
     9721 aagctttgga tctttgatac atagcgtagt gcctttcaca tagtatgtac tcaaatattg
     9781 gaagttactc atcccaaacc atagtgttta aaattggggt tttttaaaaa tgtcaagccc
     9841 ttaaagttga tcatttattt gtcaatgtaa atagaatagt aatttgtttt caaaaagatg
     9901 aggtcttgct gtggtgctca ggctggagtg cagtggctgt tcacaggcgt gataattgtg
     9961 tgttacagcc tcaaagtcca gggctcgagt gatcttctca cttcagcctc ctgagtagct
    10021 gagactaggc gtgcaccacc gtttctgaca ggattttaat ttttaagtag gtttttaacc
    10081 cacagccacg agagctaatt ttaactacat tgttagaggt agtaacagtt atataactaa
    10141 tcaaaaccag tgtgatatcc aagtactttt tttttttttt ttttttgcta tttaattttg
    10201 agataccatt ttttggaggg gtgtcctgat ttattttatt tattttattt atttattttt
    10261 attttttatt tttttagata aggtcttgct cttgcccagg ctggaatgta atggcatggt
    10321 cgcagctcac tgcagcctcc gtcttctggg ctcaagcaat cctccttcct cagcctcctg
    10381 agtagctggg actacaggtg tgtgccacca ctcccagcta atttttaaat tttgtgtaga
    10441 gatggggtct ccctgcattg cccaggttgg tctctcaaac tcctgggttc aagcagtcct
    10501 cccaccttgg cctcccaaag tgttgggatt ataggcgtaa gccactgtgc ccggctgttt
    10561 tttgttttgt tttgttttgt gttttaattg aacggactga agtttacttt ctaactaggt
    10621 tagccctcca ttcatacaaa attatgagcc caaatgtctt ggggagagta caggagaggc
    10681 cagatcttaa aagccaggag tggaattagg tttgagcata tgtagcaata tggaaacaaa
    10741 gagtgagtca atttataagc ataccaatct tcttggagac aaaagtttga attgctcacg
    10801 tgtccatttt ttttctggaa tccctgaaga aattttgaga tactgagtac tagaaaaccc
    10861 tctttgatga gggttgtata ctttattttt aaacagtatc aacaccgttg aggcccttca
    10921 agtctttgca gtcaaaagcc atacactttt tctttgagtg agcctttact ttaaggtatg
    10981 tagtagaata atataggtat ctgaaaaaga acttggttca tgacatccac tcattcattt
    11041 atgcacttag caaatattca ttgattgcca aatacgcatc agacactgag ctaggtgcta
    11101 ggtactaggt aaacaaaatt ccctgccttc atggagctta gcttgtggtg gtggaaagaa
    11161 gccctcagcc tgaatactca acttatgtct gccgtttatt ccttaacaca ttaaactata
    11221 ggtaatagca tatgttagtc taatctttgt ttagcaaagc tatttcttgt tctggaatat
    11281 aagtacagtt acagcagaat aagtaccaag gcaagattgg agaatgaccg gttgtgaaca
    11341 gggacttagg aatatgtgta gcataggatt ttattttaga gttgttaaaa tcagatcaac
    11401 agagcatgtt tttatgggcc atctgacaag actagaggta gctttggaac ctcttattcc
    11461 ctggaatgca aacatgtatt tgcatttcat ccactgtcag tatgaggaga caaaaacgaa
    11521 gtcaggattt tggagctgga atggatctta aatactatgt agtccaaacg tttggtttta
    11581 cagatgcact ttttccagag tgctgtcatc tcatatactt ttttcttcat acaggttttt
    11641 aatgtaactc actcagatcg ttggtgatag actctctctt aaaacttatt tatcctaatc
    11701 tttttttttt tttttttttt aagagacagg gttttgctct gctgtccagg cacacaatag
    11761 ctcactgtaa cctccaactc ctggagtcaa gtgatcctcc tgcctcagcc tctcaggtag
    11821 ctgggactgc actactagtg cactaagcta atttttaaat tttttttgta gagacagggt
    11881 cttgctgtgt tgcccgggct gctctcttaa ctcctggcct caagtaatcc tcttgccttg
    11941 gcttcccaaa gtgctgggat tacaggcgtg agccaccatg cttagctgat ttgccctaat
    12001 cctctgctga cattctgata tcggacaact aaaattataa cattgggcag ctattttgta
    12061 tgatatatag ggtagtggtt cttaagctta ttggtgagta accttcactt gggaagctta
    12121 tttttaaaaa tgtagacctt cattactttc ctcctgagat tttgaatcag tacctttgag
    12181 agagggtcca gtaaatcttt gttttgaatg aactgatggt tttgaagcag gtgacctgga
    12241 ggactgcatg tagaacacag tgatgtcaag gagtaaagga gagaacagaa gtgagattag
    12301 cactaatcac ctctaatgcc ttatttccag taaagtttag agaagaggaa aagctacttg
    12361 gcttgctcct ttgaaggttt tgtttccctc ttaacacaga aattaaaagg tagaggagag
    12421 atgctgaatg atgctttttt tcttcttaat cttggggggt cattatgatt aagttattga
    12481 atccatatca ccgtatgaca gcacatgatg aaagaaaatc aaatgtttgt ttcaatcaaa
    12541 gtgaaacaaa atgagaataa ctatttgggc aaaacacttt tatcactgct aagatattac
    12601 tagaactgag cagagaaata ctcagagaga gccatggcat agtactaatt aataatgggg
    12661 taccatttag caactggaat attctccgat gatgcctgtt taattaccca taggtgctaa
    12721 gtgaatgtta aaaattacgg tgctggttga gtaggccgag tagaatttgt gttagggcac
    12781 atcccatgga gatctttttt actgcttaat tttttggagt tacctcagta acttaacagg
    12841 caggaatatg gttttaatgg aaaggaaacc atttgccttt aacagcatta gtcccgctaa
    12901 gagaaagttt ttgcttgtct agtttattac aggtctttga ctttgtcagt atatggcaaa
    12961 tttagtataa aagatgatta attatcaagg ctgatgaaat ataggcagct gaaaagaaaa
    13021 ttcttagcaa ttcatacctt agaaggaacc ctgaaggaca ggacgccata gaaatcctgt
    13081 ttttttaaaa aagtaatttc aagtaagttt gcaaatgcaa tgctagtaaa aagtatatag
    13141 aggtatgtct ttcacactat cttgttacta ttagaatttt tgtagccatg tgcagaggag
    13201 agaaaaggtg ttggattttc tgcagttctg attcagttct aagaatttgg tgtgaaaaga
    13261 atgtttgcaa aggagcaggc acaaaggtga gaacaggaat acgaaacaca acatctgtaa
    13321 atgactaaat tcagagggaa tttagtgggg catatctctt aaatggtttt gaataatttg
    13381 gtgttttaac caatgactta atttaactta gtgggtatac tgttcccagt cccattactg
    13441 atttttattc ctctgtgata acatacaccc aaataaagac tgctatgtat ttgactttaa
    13501 aactataaag atgtaaaaaa ggttcactaa gccaaacacc ttagaaaata gcctcagcta
    13561 tttgctatgt ttatattata cctgcataaa agtatttatg tatagatgta cttaccttac
    13621 acagtaagta cctaaaattt gtaaattgaa tacttttgaa tgtaagcagt tgaagtaaaa
    13681 cctgtagtga gttcttcagt cagtcaacac taaacctgtt ttgtaaattt gatgctagta
    13741 ttatactgga ctttctcaaa atgaagtaca cctatatatg catatagtat atgagcatgt
    13801 gtatttgtac aaaagccctt caaaaggagt tcagctttta taaacaccaa aacactctct
    13861 gcctgtaaaa tgtttttgct gaaatttgta tcattaactc tcaaatttac atcttcatgt
    13921 ttgagatacg cttttaggac tgtctatgca tgtagacttt ggtcaactct ctcctcctcc
    13981 ctcaataaat cagttaactt aaaaaatata ttgtgaccat ttttataaaa tacatgttca
    14041 taaaacagat caacatattt agcttataca gaaataaaat taagtcaatc cactcacaaa
    14101 gaatttctat tttgtaaaaa tgtagcttgt atttcagtat aataaaatct gatgtaagaa
    14161 a
//
