LOCUS       NM_001130912           14257 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly
            cochaperone (SUGT1), transcript variant 1, mRNA.
ACCESSION   NM_001130912
VERSION     NM_001130912.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Bohush,A., Goral,A., Sierant,M., Nawrot,B., Lesniak,W. and
            Filipek,A.
  TITLE     Sgt1 Regulates alpha-Synuclein Subcellular Localization and
            Expression of Parkinson's Disease Related Genes, PINK1 and PARK9
  JOURNAL   Biomolecules 11 (11), 1675 (2021)
   PUBMED   34827672
  REMARK    GeneRIF: Sgt1 Regulates alpha-Synuclein Subcellular Localization
            and Expression of Parkinson's Disease Related Genes, PINK1 and
            PARK9.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  AUTHORS   Allouch,A., Di Primio,C., Paoletti,A., Le-Bury,G., Subra,F.,
            Quercioli,V., Nardacci,R., David,A., Saidi,H., Cereseto,A.,
            Ojcius,D.M., Montagnac,G., Niedergang,F., Pancino,G.,
            Saez-Cirion,A., Piacentini,M., Gougeon,M.L., Kroemer,G. and
            Perfettini,J.L.
  TITLE     SUGT1 controls susceptibility to HIV-1 infection by stabilizing
            microtubule plus-ends
  JOURNAL   Cell Death Differ 27 (12), 3243-3257 (2020)
   PUBMED   32514048
  REMARK    GeneRIF: SUGT1 controls susceptibility to HIV-1 infection by
            stabilizing microtubule plus-ends.
REFERENCE   3  (bases 1 to 14257)
  AUTHORS   Filipek,A. and Lesniak,W.
  TITLE     Current view on cellular function of S100A6 and its ligands,
            CacyBP/SIP and Sgt1
  JOURNAL   Postepy Biochem 64 (3), 242-252 (2018)
   PUBMED   30656909
  REMARK    GeneRIF: REVIEW: cellular function of S100A6 and its ligands,
            CacyBP/SIP and Sgt1
            Review article
REFERENCE   4  (bases 1 to 14257)
  AUTHORS   Niikura,Y., Kitagawa,R., Ogi,H. and Kitagawa,K.
  TITLE     SGT1-HSP90 complex is required for CENP-A deposition at centromeres
  JOURNAL   Cell Cycle 16 (18), 1683-1694 (2017)
   PUBMED   28816574
  REMARK    GeneRIF: the SGT1-HSP90 complex contributes to the E3 ligase
            activity of the CUL4A complex that is necessary for CENP-A
            ubiquitylation and CENP-A deposition at the centromere.
REFERENCE   5  (bases 1 to 14257)
  AUTHORS   Steensgaard,P., Garre,M., Muradore,I., Transidico,P., Nigg,E.A.,
            Kitagawa,K., Earnshaw,W.C., Faretta,M. and Musacchio,A.
  TITLE     Sgt1 is required for human kinetochore assembly
  JOURNAL   EMBO Rep 5 (6), 626-631 (2004)
   PUBMED   15133482
REFERENCE   6  (bases 1 to 14257)
  AUTHORS   Lee,Y.T., Jacob,J., Michowski,W., Nowotny,M., Kuznicki,J. and
            Chazin,W.J.
  TITLE     Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the
            tetratricopeptide repeat domain
  JOURNAL   J Biol Chem 279 (16), 16511-16517 (2004)
   PUBMED   14761955
REFERENCE   7  (bases 1 to 14257)
  AUTHORS   Zou,X., Ji,C., Wang,L., Wu,M., Zheng,H., Xu,J., Jin,F., Gu,S.,
            Ying,K., Xie,Y. and Mao,Y.
  TITLE     Molecular cloning and characterization of SGT1.2, a novel splice
            variant of Homo sapiens SGT1
  JOURNAL   DNA Seq 15 (2), 140-143 (2004)
   PUBMED   15346769
  REMARK    GeneRIF: SGt1.2 is a splicing variant of SGT1 and widely expressed
            in human adult tissue.
REFERENCE   8  (bases 1 to 14257)
  AUTHORS   Niikura,Y. and Kitagawa,K.
  TITLE     Identification of a novel splice variant: human SGT1B (SUGT1B)
  JOURNAL   DNA Seq 14 (6), 436-441 (2003)
   PUBMED   15018354
REFERENCE   9  (bases 1 to 14257)
  AUTHORS   Nowotny,M., Spiechowicz,M., Jastrzebska,B., Filipek,A., Kitagawa,K.
            and Kuznicki,J.
  TITLE     Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and
            other S100 proteins
  JOURNAL   J Biol Chem 278 (29), 26923-26928 (2003)
   PUBMED   12746458
  REMARK    GeneRIF: Sgt1 binds to S100A6 in a calcium-regulated manner
REFERENCE   10 (bases 1 to 14257)
  AUTHORS   Kitagawa,K., Skowyra,D., Elledge,S.J., Harper,J.W. and Hieter,P.
  TITLE     SGT1 encodes an essential component of the yeast kinetochore
            assembly pathway and a novel subunit of the SCF ubiquitin ligase
            complex
  JOURNAL   Mol Cell 4 (1), 21-33 (1999)
   PUBMED   10445024
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139089.13.
            
            On Jun 2, 2019 this sequence version replaced NM_001130912.2.
            
            Summary: This gene encodes a highly conserved nuclear protein
            involved in kinetochore function and required for the G1/S and G2/M
            transitions. This protein interacts with heat shock protein 90.
            Alternative splicing results in multiple transcript variants.
            Pseudogenes for this gene have been defined on several different
            chromosomes. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1, also known as B or SGT1.2)
            encodes the longest isoform (B).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.31332.1,
                                           SRR1803614.94755.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AL139089.13        8419-8541
            124-181             AL139089.13        8629-8686
            182-272             AL139089.13        13115-13205
            273-342             AL139089.13        13982-14051
            343-413             AL139089.13        14762-14832
            414-509             AL139089.13        17058-17153
            510-563             AL139089.13        18232-18285
            564-580             AL139089.13        18679-18695
            581-603             AL139089.13        19618-19640
            604-700             AL139089.13        21220-21316
            701-808             AL139089.13        22395-22502
            809-899             AL139089.13        31813-31903
            900-1081            AL139089.13        35557-35738
            1082-14257          AL139089.13        43317-56492
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.3"
     gene            1..14257
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="SGT1 homolog, MIS12 kinetochore complex assembly
                     cochaperone"
                     /db_xref="GeneID:10910"
                     /db_xref="HGNC:HGNC:16987"
                     /db_xref="MIM:604098"
     exon            1..123
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1183
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="isoform B is encoded by transcript variant 1;
                     suppressor of G2 allele of SKP1, S. cerevisiae, homolog
                     of; putative 40-6-3 protein; protein SGT1 homolog; SGT1,
                     suppressor of G2 allele of SKP1"
                     /codon_start=1
                     /product="protein SGT1 homolog isoform B"
                     /protein_id="NP_001124384.1"
                     /db_xref="CCDS:CCDS45050.1"
                     /db_xref="GeneID:10910"
                     /db_xref="HGNC:HGNC:16987"
                     /db_xref="MIM:604098"
                     /translation="MAAAAAGTATSQRFFQSFSDALIDEDPQAALEELTKALEQKPDD
                     AQYYCQRAYCHILLGNYCVAVADAKKSLELNPNNSTAMLRKGICEYHEKNYAAALETF
                     TEGQKLDIETGFHRVGQAGLQLLTSSDPPALDSQSAGITGADANFSVWIKRCQEAQNG
                     SESEVWTHQSKIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDY
                     NLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNL
                     YPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFME
                     SGGTVLSTNWSDVGKRKVEINPPDDMEWKKY"
     exon            124..181
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            182..272
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            273..342
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            343..413
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..509
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            510..563
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            564..580
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            581..603
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            604..700
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            701..808
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            809..899
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1081
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..14257
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1183..1188
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATAAA"
     polyA_site      1203
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="major polyA site"
     regulatory      1623..1628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATACA"
     polyA_site      1641
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="major polyA site"
     regulatory      14080..14085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATAAA"
     polyA_site      14102
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
     regulatory      14237..14242
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
                     /note="hexamer: AATAAA"
     polyA_site      14257
                     /gene="SUGT1"
                     /gene_synonym="SGT1"
ORIGIN      
        1 ctccagaagt ttcccccttg ggcggtggtg gaggtggtaa ccgtgatagt agcagctccg
       61 gcggcagcaa cagcgactac gagggatggc ggcggctgca gcaggaactg caacatccca
      121 gaggtttttc cagagcttct cggatgccct aatcgacgag gacccccagg cggcgttaga
      181 ggagctgact aaggctttgg aacagaaacc agatgatgca cagtattatt gtcaaagagc
      241 ttattgtcac attcttcttg ggaattactg tgttgctgtt gctgatgcaa agaagtctct
      301 agaactcaat ccaaataatt ccactgctat gctgagaaaa ggaatatgtg aataccatga
      361 aaaaaactat gctgctgccc tagaaacttt tacagaagga caaaaattag atatagagac
      421 ggggtttcat cgtgttggcc aggctggtct ccaactcttg acctcaagtg atccacctgc
      481 cttggactcc caaagtgctg ggattacagg tgcagatgct aatttcagtg tctggattaa
      541 aaggtgtcaa gaagctcaga atggctcaga atctgaggtg tggactcatc agtcaaaaat
      601 caagtatgac tggtatcaaa cagaatctca agtagtcatt acacttatga tcaagaatgt
      661 tcagaagaat gatgtaaatg tggaattttc agaaaaagag ttgtctgctt tggttaaact
      721 tccttctgga gaggattaca atttgaaact ggaacttctt catcctataa taccagaaca
      781 gagcacgttt aaagtacttt caacaaagat tgaaattaaa ctgaaaaagc cagaggctgt
      841 gagatgggaa aagctagagg ggcaaggaga tgtgcctacg ccaaaacaat tcgtagcaga
      901 tgtaaagaac ctatatccat catcatctcc ttatacaaga aattgggata aattggttgg
      961 tgagatcaaa gaagaagaaa agaatgaaaa gttggaggga gatgcagctt taaacagatt
     1021 atttcagcag atctattcag atggttctga tgaagtgaaa cgtgccatga acaaatcctt
     1081 tatggagtcg ggtggtacag ttttgagtac caactggtct gatgtaggta aaaggaaagt
     1141 tgaaatcaat cctcctgatg atatggaatg gaaaaagtac taaataaatt aatttgctct
     1201 catcgtattg tgtatattca cctaatgccc attgtgtatt gatattgcat tcttgaattt
     1261 tgaacactga atatcttttt gaaagattat acttctttac ctctttgtgc tttagaaatt
     1321 attttccttc aagtgttcaa gtctaatgaa gaatgaagat aacattttat cacttctgtc
     1381 cttaaaggtt tcagacatgg tgaaactgaa taaagcatgt catttgctcc tagatagatt
     1441 cattctatct agttgtgggg atggagaaat ctttaatggt atattttcgg ttattgcctt
     1501 atttttgata cagtattctg tcagtaattt attagacctg gcagctttgg gtgagcttag
     1561 atttttcacc ttcagtgtta cattgtgttt gcttttaaaa actgcttttg aatggagttg
     1621 taaatacaat ttttctatga agatgtttgg tttatttacc tttttcttaa gtttttaatg
     1681 agaactttga gaaattatca cagggaattc ccatgtagtt cttttaatgg gaagaatttg
     1741 taaatttaat tgcactgaga tattttggtc gttaccttgc aagtttctgt ataaaacata
     1801 gatacctgag ttttaacaca ctgcaagttt atactaagga taatctgtga aggtagggca
     1861 aagaaggttc taggaaagcc caactccact tgcttctttt atcacgacag gattcttgaa
     1921 attacagcat tggtgggttt tttggtattt tttttcttta ttagaaaatc tctgttgtca
     1981 cgtccccatg attactttgg tttcattggt ttatggtaac tgctgtgaga gtaaattaat
     2041 gtgtatatgt ttagtattta ctctgtgcct agaatatata gatatcatga gaatacaaaa
     2101 aattaaagac atgattatta acttcaaaga gcttataaat gtagcaggga aaaaaaataa
     2161 cattcatgaa ggaactgtgg aaagattgaa tgctgaactc ggtataaata cactttagag
     2221 acaggaaaat gttctgtgat taaaattgtt tacagtctag gctgttataa agtatagact
     2281 atggatgacc tgtagatctg actttgtttt ctcaccatga ttttctcaac tgtaataccc
     2341 cagatgttgg tctcagctct gttaggtgtc actaacattt ttatggcaaa gcactactct
     2401 ataccctcag agcaggggga gtgggaggca aacgggtcat catttcagcc tccaaaacag
     2461 taggaattca gctccttcct tctcgtcctt tgttgtgggg tgtaggggag ttactaagga
     2521 aagtttcagt aaggtgatac cagatggtgc ccaaacctgt tgttatagct tgtttaagca
     2581 ttctagagtt ttcacttgtc cttctgtcca cttagagcta ctagatacat ttttttcttc
     2641 ttgaacactg aaggaaaagg caaaattaag ttttggttgg atggatttct aaagttttct
     2701 tcttaggaaa ataggatagt gacttttgta ttctgtatgt taaattttct cagtaccatt
     2761 aatcctaaga tgaagaggaa ggctattgct gcagaggcat agttgaggac gtatagcact
     2821 ataccagaga caagaatgct tagctctagc aagtcttctg tcattgacta ccttcataat
     2881 cttcagcagg tttaggcctt tcttagttcc tttgtaaata atccatagag tcttagagtt
     2941 ataattaaag gtccatggtt ctaaatacag aagcatattt ttgccgctta actttttctt
     3001 tcttaaagat gacactatta aaatagctgt ttctgaccag gtgcagtagc tcatgcttgt
     3061 aatcccagca ttttgggagg cggaggtggg cagatcacct gaggtcagga gttcaagacc
     3121 agcctggcca acatggtgaa accctgtgtc tactaaaaat gcaaaaatta gctgggtgta
     3181 gtggcatgca cctgtagtcc cagctacttg ggaggctcag gcagggagaa ttgcttgaac
     3241 cgggaggcag ggggtgcagt gagccaagat catgccactg cactccagcc tgggcgactc
     3301 catctcaaaa aaaataaata gctgtttaaa aaaaaaaaat ctggtactat ctttagttgg
     3361 ttttaaagga tatattatct agaatatgct tacccattgg ctagatttgc taagtgttgt
     3421 gggatttggg attttgctgg tctgaaggga gtttgtaaaa cacatgactg aaagcattat
     3481 aaaacatacc ttgtcaaata cattagattc attataggtc aagggaattt tgaaaagtag
     3541 gctagattgc tgcctaagaa tcccactcta aagtactgct ttgtttaatc attctagata
     3601 atggaagctt gtaagcctcc agaaagttaa aacaaaaaac ttctttctga ttaagagcct
     3661 cttaactcta tccttatcct ctgctatctc aatgtagcta ttaattgttc cctctttgtt
     3721 tctagactcc aattttcatt tctcaaattt tggttctcag tgctgttagt attagtagcc
     3781 ttttgtttct tcagaatgta tgtatttttc ctcttaaatg tttcctttcc cttttaaggt
     3841 tagtcctgga atacatgttt ttcttggtct tgtagacttc atctcttctg atttccataa
     3901 taatttgcac cttgatgaat ccagaattct cccactgtct tcaaagaatc ttgaatagaa
     3961 taatctgctt tttgaactct gctatctagt aatctcttta gatatctata ccacaagttt
     4021 gaaacatcaa ggtcaatttt gataatttcc tgtcttatgt cttaataccc attcagagtt
     4081 gactgccaac taacaagctc agcctttagc actggcactt actggccaat ctgttttctg
     4141 tctataggga gtgaaatgga atggagaaag aggatggctt gcattaataa ctctagtttt
     4201 taaaaacatt tttattcctt ttttaaaaat ttgtttagag acacggtctt cttctatcac
     4261 ctaggctgga gtgcagtggt gcgatcatgg ctcactgtag cctccaactc ctaggctcaa
     4321 gtaatcctct ttcctcggct tcccaaagtg ctgggattac aggtgtgagt cactgtgcct
     4381 ggcccacatt ttcattcttg gctcactgta gccttaatct ttcaggctca aatgattctc
     4441 ccatctcagc ctcccaagta gctgggacca caggcatgca tcaccacgac tggctaattt
     4501 ttgtattttt tttgtagaac cgggtttcac catgtgccca ggctggtctt gaacttctgg
     4561 gctcaagtta tctacctgcc tcagcctacc caaagtgctg ggattacagg agtgagccac
     4621 catgcccagt tatgaacttc aagtaaatat ttaaaatgga gatacatcac aatgctatta
     4681 tgaataattt agatagaaat tttaatttag ttcagtactt aaatattttt tggctcttct
     4741 ttcccacttt acctttctct attccttcct cagttatcta catatcagat tagattattg
     4801 atatctgaga tatatcactg acccagagca aaattgcata ttagtttacc atctaatcag
     4861 cctaatgcac ttaaattccc ttggaatctt tcctgttcag attggtgtgc atctctctga
     4921 acttgtagct ttactgacat ttccaaaaaa aggtagacgg tttctaggtg gtgtgtgttt
     4981 ttgttagtta ctagagccac ttaccctttg taactagctc taaagaagta catcttttgc
     5041 tcatacctgc tactgtaacc atatgacagt atggatttaa gttatttctg cctagaggaa
     5101 ttagatgata aaagctaaaa ggacaaaata ctggtttctg tgcagggaag ggtcatatgt
     5161 agttttaaat aaaagggttc atctcttttg atgtggtttc cctttctcca tctgtgcatt
     5221 ttttaaatcg taagatcatt ttacacacac caagagattt tattttggga acttgtgggg
     5281 aaaatattga tttacctaca aatgttacta tttggagcca tccttgtgat tggtttctga
     5341 gattaattta actcttagcc tcacttccta ccttagtaaa gttgctcctc caaatcagct
     5401 aaaatcatat acaatgaatg ctgactcatt actgcaaata gtggtagtta ctgtgtacta
     5461 gcatttctta tggattgggt ggcattatct catttttaat cgcccttcaa tagcatccca
     5521 ttattgcaaa tcagaaagat gatggggcca aagtaaagtt ctttgcccag gtcactctac
     5581 tagtaagtaa ttgcaatagc tgggatttga acccaggcct atcagactcc attacccata
     5641 cttctgacaa ctgcaatcct ctcatcaggt taataagcta gatgtggaat gaatgatttt
     5701 tattaccaaa tcagctgact ggaaatgaag cagagaatat tcaagttaaa gaactaattc
     5761 tttttttttt tttttttgag acagtctcac tctgtcgttc agggaaggaa tgcagtggtg
     5821 caatcttggc tcactgcaac ctccacctcc caggttcaag taattcttct gcctcagcct
     5881 cctgagtagc tgggattaca ggcatgggcc gccatgccca gctaattttt gtatttttag
     5941 tagagacgag gtttcaccat gttggtcagg ctggtcttga actcctgacc tcatgatccg
     6001 cccaccttgg cctcccaaaa tgctgggatt ataggcgtga gccactgtgc ccagccaaga
     6061 actaattctt atataggagc tgggaagata aattcctaga gatcctcact ttcttaagta
     6121 ggaaagctta ttaggaatcc aaatcttttg cctcatactg gaagtaatgc aaagaagtag
     6181 tctgattttt gagagggtgc tgtttttctc aaatctagat cattacagag aaacagtgta
     6241 cttttaacaa ctgttgcatg tgtaagaaga ctggtacatg atgccaatga aattcttttt
     6301 ttcctttaac ttgggagctc tcataccagt tccgttgtac aaatgctgag attgtttcaa
     6361 ggttttattc ctacagacat gttaggattt gtgtatcact gatttctcca ataaaatttg
     6421 aatatttcag gttatttcct ttttcctaac ttatgttagc attttgtata tatatatata
     6481 tttgaatttt ggcttgtctg gagtcttaaa tatttggtat gccttcacat ttggattttt
     6541 agttttattt ttacattgag acttctacct atatcattga ctttattttt tcctatgatt
     6601 aaaggattca gttatacaaa aagatgtttc agaaacactg ctatttacct aaaggtaatt
     6661 ttcagtttct ttagaaggag ttaggaaaat ggtccttaat acctatatag ggttttttaa
     6721 aaaactgtac cctataatgc agccacagaa gatctaaata ataaacaatt gcgcatacta
     6781 ttaatatata tttagagctt ttgttaagta catgaaaaga cagtattgca tagtggttaa
     6841 atgcatggac tctgaagcca tcctgtttgg gatcatcctg gctacattgc ttactagctg
     6901 tgtgacaatg gatataggtg cttaatgtct ctgtgcctca gtttttgtgt ctatgtaaag
     6961 gagataatag gatccccctc atgcctcatg gtcttagttg aatgaattca tgtaactcca
     7021 caggtatctt aatccagaag gctcaaaata ctaatgtttt gtgtgtgtgt gtgtgtgtgc
     7081 atgtgcatct gtatgacaag tgatataaaa cgtgacctga atttataata tgaatagtca
     7141 cattttgcag cagaaatatt aaatgtttaa ttatagaatg ctagcactga tcctgctgaa
     7201 attgttacaa aatacagcag ttgcccctta actgtggcag atacattcca agacccccag
     7261 tactgaatgc tgtatgtaca ttttttatac atgtatactt atgataaagt ttataaatta
     7321 ggcacagtaa gatattaata atagaatata caatataccg caataaaact tatgtgaaca
     7381 gttatttttg gacagcagtt gactgtgggt cactgaaatt gtggaaagca aaaccatgga
     7441 taaaggggaa ctattaatag tctatctgta ttgcattatc ttctaaaatc caaaattctg
     7501 acttcaaaaa ctcatctgag ggttttggaa ttgtgtatct atgtgtgtga agtgcaaaga
     7561 gcagtgcttc atgtgacaga taacatcaga tgatcctagg ctggaaggtg gaaatcctag
     7621 agaagatttt tttgtgttgt aagttcagtc tttcaagatg tggaattctg tctgaggaaa
     7681 gtatatcacc tagttaacaa ttgttactgt aatttataaa atttgtactt tggagagcat
     7741 aagaattcat gaaaagtttt taataatttt acttaaacag agcttaatta tgtaagtatt
     7801 gtcagcactt tataaagaat cgtaaaataa aatagtggca gaaaatataa actagatgta
     7861 gaatgttaag gaactttcgg aagttctctg gttttcattt caatttcaaa gctcaaaaca
     7921 gaaggtaaaa ctattaggat ttgtgaaaaa tacaaggtac aaaatcacgc agtttataaa
     7981 ccacagattg tcttgttatt cccatcagtg aaaagaagct gatatttgtt tgtgtttgtt
     8041 tttgtttttg tttttgagac ggagtcttgc tctgtcgccc aggctggagt gcagtggcgc
     8101 cattttggct cactgcaagc tccgcctccc ggttcacgcc attctcatgc ctcagcctcc
     8161 caagtagctg ggactacagg cgtccgccac catgcccggc taattttttg tatttttagt
     8221 agagacaaga gtttcaccgt gttagccagg atggtctcat ctcctgatct tgtgacccgc
     8281 ccgcctcggc ctcccaaagt gctgggatta caggcgtgag ccaccatgcc cagcctgctg
     8341 gtagatgttt ttatgagtta attcatcaat cttattattc ttgccccaaa tgaatcagtg
     8401 tggagcatat acaagtgtgt agacattaat atgggctcac tgtcagctgg gagttctcac
     8461 aactaccact ttttactgat tatctgtttt ctaaactttt tctgtggctt gaagtgactg
     8521 aaagtgaaca cattcataag taatgagctt aggctgagga tatatatcca gtgggggatg
     8581 aaacatgctt aagaaattga gtgcatttcc tttattattg ttattatttt gctatttcaa
     8641 aacaattgac tttaaaaaat tatacttcac cacagagaca ggtgttgttt tgtaatggaa
     8701 ggagtagtaa cttatgaatc tagactctta aaatcccagt tctgatactt tcataattgt
     8761 gtgttttgag ggcaagtgac ttatctgaac ttttttcttt tttataacac tggatgataa
     8821 ttaaccctca ggattgtgag gatttaataa ctaagtcctg actcaaatta tgttaccaat
     8881 aaatgctact taaatgtaaa gtccttgatt tattttttcc cctcagtcct gtttagtgtt
     8941 gaaattcttc aaaggagggt gagtctggca tttacctgct attcaagagt tttcagaatt
     9001 caaccccagc caaaattttt ggccacatct cttgtcactg ctttgctgtg tactaaagct
     9061 cccagacaca caagaatctt ctagtcatgg tcaaactttt tctgcaaagg gcacttacag
     9121 tctattgcat attctttgtt tttagtgttc agacatctgc tgtttctgaa tatgtcgtgt
     9181 attttcattc ctttttctgc ccctcctggg catctccagt ttcctactga tgtgtcaaaa
     9241 ctgagtttgt atgctacctc caggaaagca gtctaatctt ttgcaactct gttactgact
     9301 tttgtggcat tggattgggt tatttgctta tggttgagac atcactggat cctttgatgg
     9361 agtcatacca aaaccacaac atgtcatgag catgcttgag agtgctaatc tattccttgt
     9421 ttgtcagacc agtacattac agttggttca tttcttcccc cgtgtgactg agtttcttga
     9481 gggcgcggtc tagcttccat ctttgtatgt gtagcacctg gtacatagag aacaggctcc
     9541 cagaattgga tggatttgct catggtctta tcactgcaca gaatgccctt attttcttcc
     9601 attcattcta tgagatctca attaaaatgt cagtactctt gtggaactcc cctctccatg
     9661 ccctctactg aaatgcattg ccactctagt tgtgatgtgg gagtgccttg tttatgcctc
     9721 tatttatgac accttcatgg tgtcacatgt tatagggtta gttgcggctg tgtctgtttt
     9781 accttgtgtg tcttggtcag ttagtgttat ttacttaagc tttggatctt tgatacatag
     9841 cgtagtgcct ttcacatagt atgtactcaa atattggaag ttactcatcc caaaccatag
     9901 tgtttaaaat tggggttttt taaaaatgtc aagcccttaa agttgatcat ttatttgtca
     9961 atgtaaatag aatagtaatt tgttttcaaa aagatgaggt cttgctgtgg tgctcaggct
    10021 ggagtgcagt ggctgttcac aggcgtgata attgtgtgtt acagcctcaa agtccagggc
    10081 tcgagtgatc ttctcacttc agcctcctga gtagctgaga ctaggcgtgc accaccgttt
    10141 ctgacaggat tttaattttt aagtaggttt ttaacccaca gccacgagag ctaattttaa
    10201 ctacattgtt agaggtagta acagttatat aactaatcaa aaccagtgtg atatccaagt
    10261 actttttttt tttttttttt ttgctattta attttgagat accatttttt ggaggggtgt
    10321 cctgatttat tttatttatt ttatttattt atttttattt tttatttttt tagataaggt
    10381 cttgctcttg cccaggctgg aatgtaatgg catggtcgca gctcactgca gcctccgtct
    10441 tctgggctca agcaatcctc cttcctcagc ctcctgagta gctgggacta caggtgtgtg
    10501 ccaccactcc cagctaattt ttaaattttg tgtagagatg gggtctccct gcattgccca
    10561 ggttggtctc tcaaactcct gggttcaagc agtcctccca ccttggcctc ccaaagtgtt
    10621 gggattatag gcgtaagcca ctgtgcccgg ctgttttttg ttttgttttg ttttgtgttt
    10681 taattgaacg gactgaagtt tactttctaa ctaggttagc cctccattca tacaaaatta
    10741 tgagcccaaa tgtcttgggg agagtacagg agaggccaga tcttaaaagc caggagtgga
    10801 attaggtttg agcatatgta gcaatatgga aacaaagagt gagtcaattt ataagcatac
    10861 caatcttctt ggagacaaaa gtttgaattg ctcacgtgtc catttttttt ctggaatccc
    10921 tgaagaaatt ttgagatact gagtactaga aaaccctctt tgatgagggt tgtatacttt
    10981 atttttaaac agtatcaaca ccgttgaggc ccttcaagtc tttgcagtca aaagccatac
    11041 actttttctt tgagtgagcc tttactttaa ggtatgtagt agaataatat aggtatctga
    11101 aaaagaactt ggttcatgac atccactcat tcatttatgc acttagcaaa tattcattga
    11161 ttgccaaata cgcatcagac actgagctag gtgctaggta ctaggtaaac aaaattccct
    11221 gccttcatgg agcttagctt gtggtggtgg aaagaagccc tcagcctgaa tactcaactt
    11281 atgtctgccg tttattcctt aacacattaa actataggta atagcatatg ttagtctaat
    11341 ctttgtttag caaagctatt tcttgttctg gaatataagt acagttacag cagaataagt
    11401 accaaggcaa gattggagaa tgaccggttg tgaacaggga cttaggaata tgtgtagcat
    11461 aggattttat tttagagttg ttaaaatcag atcaacagag catgttttta tgggccatct
    11521 gacaagacta gaggtagctt tggaacctct tattccctgg aatgcaaaca tgtatttgca
    11581 tttcatccac tgtcagtatg aggagacaaa aacgaagtca ggattttgga gctggaatgg
    11641 atcttaaata ctatgtagtc caaacgtttg gttttacaga tgcacttttt ccagagtgct
    11701 gtcatctcat atactttttt cttcatacag gtttttaatg taactcactc agatcgttgg
    11761 tgatagactc tctcttaaaa cttatttatc ctaatctttt tttttttttt ttttttaaga
    11821 gacagggttt tgctctgctg tccaggcaca caatagctca ctgtaacctc caactcctgg
    11881 agtcaagtga tcctcctgcc tcagcctctc aggtagctgg gactgcacta ctagtgcact
    11941 aagctaattt ttaaattttt tttgtagaga cagggtcttg ctgtgttgcc cgggctgctc
    12001 tcttaactcc tggcctcaag taatcctctt gccttggctt cccaaagtgc tgggattaca
    12061 ggcgtgagcc accatgctta gctgatttgc cctaatcctc tgctgacatt ctgatatcgg
    12121 acaactaaaa ttataacatt gggcagctat tttgtatgat atatagggta gtggttctta
    12181 agcttattgg tgagtaacct tcacttggga agcttatttt taaaaatgta gaccttcatt
    12241 actttcctcc tgagattttg aatcagtacc tttgagagag ggtccagtaa atctttgttt
    12301 tgaatgaact gatggttttg aagcaggtga cctggaggac tgcatgtaga acacagtgat
    12361 gtcaaggagt aaaggagaga acagaagtga gattagcact aatcacctct aatgccttat
    12421 ttccagtaaa gtttagagaa gaggaaaagc tacttggctt gctcctttga aggttttgtt
    12481 tccctcttaa cacagaaatt aaaaggtaga ggagagatgc tgaatgatgc tttttttctt
    12541 cttaatcttg gggggtcatt atgattaagt tattgaatcc atatcaccgt atgacagcac
    12601 atgatgaaag aaaatcaaat gtttgtttca atcaaagtga aacaaaatga gaataactat
    12661 ttgggcaaaa cacttttatc actgctaaga tattactaga actgagcaga gaaatactca
    12721 gagagagcca tggcatagta ctaattaata atggggtacc atttagcaac tggaatattc
    12781 tccgatgatg cctgtttaat tacccatagg tgctaagtga atgttaaaaa ttacggtgct
    12841 ggttgagtag gccgagtaga atttgtgtta gggcacatcc catggagatc ttttttactg
    12901 cttaattttt tggagttacc tcagtaactt aacaggcagg aatatggttt taatggaaag
    12961 gaaaccattt gcctttaaca gcattagtcc cgctaagaga aagtttttgc ttgtctagtt
    13021 tattacaggt ctttgacttt gtcagtatat ggcaaattta gtataaaaga tgattaatta
    13081 tcaaggctga tgaaatatag gcagctgaaa agaaaattct tagcaattca taccttagaa
    13141 ggaaccctga aggacaggac gccatagaaa tcctgttttt ttaaaaaagt aatttcaagt
    13201 aagtttgcaa atgcaatgct agtaaaaagt atatagaggt atgtctttca cactatcttg
    13261 ttactattag aatttttgta gccatgtgca gaggagagaa aaggtgttgg attttctgca
    13321 gttctgattc agttctaaga atttggtgtg aaaagaatgt ttgcaaagga gcaggcacaa
    13381 aggtgagaac aggaatacga aacacaacat ctgtaaatga ctaaattcag agggaattta
    13441 gtggggcata tctcttaaat ggttttgaat aatttggtgt tttaaccaat gacttaattt
    13501 aacttagtgg gtatactgtt cccagtccca ttactgattt ttattcctct gtgataacat
    13561 acacccaaat aaagactgct atgtatttga ctttaaaact ataaagatgt aaaaaaggtt
    13621 cactaagcca aacaccttag aaaatagcct cagctatttg ctatgtttat attatacctg
    13681 cataaaagta tttatgtata gatgtactta ccttacacag taagtaccta aaatttgtaa
    13741 attgaatact tttgaatgta agcagttgaa gtaaaacctg tagtgagttc ttcagtcagt
    13801 caacactaaa cctgttttgt aaatttgatg ctagtattat actggacttt ctcaaaatga
    13861 agtacaccta tatatgcata tagtatatga gcatgtgtat ttgtacaaaa gcccttcaaa
    13921 aggagttcag cttttataaa caccaaaaca ctctctgcct gtaaaatgtt tttgctgaaa
    13981 tttgtatcat taactctcaa atttacatct tcatgtttga gatacgcttt taggactgtc
    14041 tatgcatgta gactttggtc aactctctcc tcctccctca ataaatcagt taacttaaaa
    14101 aatatattgt gaccattttt ataaaataca tgttcataaa acagatcaac atatttagct
    14161 tatacagaaa taaaattaag tcaatccact cacaaagaat ttctattttg taaaaatgta
    14221 gcttgtattt cagtataata aaatctgatg taagaaa
//
