LOCUS       NM_145251               4864 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens serine/threonine/tyrosine interacting protein (STYX),
            transcript variant 1, mRNA.
ACCESSION   NM_145251 XM_001125680
VERSION     NM_145251.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Liu,L., Jiang,H., Wang,X., Wang,X. and Zou,L.
  TITLE     STYX/FBXW7 axis participates in the development of endometrial
            cancer cell via Notch-mTOR signaling pathway
  JOURNAL   Biosci Rep 40 (4) (2020)
   PUBMED   32239181
  REMARK    GeneRIF: STYX/FBXW7 axis participates in the development of
            endometrial cancer cell via Notch-mTOR signaling pathway.
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   He,D., Ma,Z., Fang,C., Ding,J., Yang,W., Chen,P., Huang,L.,
            Wang,C., Yu,Y., Yang,L., Li,Y. and Zhou,Z.
  TITLE     Pseudophosphatase STYX promotes tumor growth and metastasis by
            inhibiting FBXW7 function in colorectal cancer
  JOURNAL   Cancer Lett 454, 53-65 (2019)
   PUBMED   30981757
  REMARK    GeneRIF: STYX bound to the F-box and WD repeat domain-containing7
            (FBXW7) protein and inhibited its function. Co-regulation of STYX
            and FBXW7 expression reversed the biological changes mediated by
            regulation of STYX expression alone in CRC cells.
REFERENCE   3  (bases 1 to 4864)
  AUTHORS   Reiterer,V., Pawlowski,K. and Farhan,H.
  TITLE     STYX: a versatile pseudophosphatase
  JOURNAL   Biochem Soc Trans 45 (2), 449-456 (2017)
   PUBMED   28408485
  REMARK    GeneRIF: Results suggest that STYX is a pseudophosphatase that uses
            the 'competitor' and 'anchor' mode of action to exert its biologic
            roles. [review]
            Review article
REFERENCE   4  (bases 1 to 4864)
  AUTHORS   Reiterer,V., Figueras-Puig,C., Le Guerroue,F., Confalonieri,S.,
            Vecchi,M., Jalapothu,D., Kanse,S.M., Deshaies,R.J., Di Fiore,P.P.,
            Behrends,C. and Farhan,H.
  TITLE     The pseudophosphatase STYX targets the F-box of FBXW7 and inhibits
            SCFFBXW7 function
  JOURNAL   EMBO J 36 (3), 260-273 (2017)
   PUBMED   28007894
  REMARK    GeneRIF: The authors show that STYX binds to the F-box domain of
            FBXW7 and disables its recruitment into the SCF ubiquitin ligase
            complex.
            Erratum:[EMBO J. 2018 Mar 15;37(6):e99170. doi:
            10.15252/embj.201899170.. PMID: 29545456]
REFERENCE   5  (bases 1 to 4864)
  AUTHORS   St-Denis,N., Gupta,G.D., Lin,Z.Y., Gonzalez-Badillo,B., Veri,A.O.,
            Knight,J.D.R., Rajendran,D., Couzens,A.L., Currie,K.W., Tkach,J.M.,
            Cheung,S.W.T., Pelletier,L. and Gingras,A.C.
  TITLE     Phenotypic and Interaction Profiling of the Human Phosphatases
            Identifies Diverse Mitotic Regulators
  JOURNAL   Cell Rep 17 (9), 2488-2501 (2016)
   PUBMED   27880917
REFERENCE   6  (bases 1 to 4864)
  AUTHORS   Kuzmin,A., Jarvi,K., Lo,K., Spencer,L., Chow,G.Y., Macleod,G.,
            Wang,Q. and Varmuza,S.
  TITLE     Identification of potentially damaging amino acid substitutions
            leading to human male infertility
  JOURNAL   Biol Reprod 81 (2), 319-326 (2009)
   PUBMED   19369647
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 4864)
  AUTHORS   Yatsenko,A.N., Roy,A., Chen,R., Ma,L., Murthy,L.J., Yan,W.,
            Lamb,D.J. and Matzuk,M.M.
  TITLE     Non-invasive genetic diagnosis of male infertility using
            spermatozoal RNA: KLHL10 mutations in oligozoospermic patients
            impair homodimerization
  JOURNAL   Hum Mol Genet 15 (23), 3411-3419 (2006)
   PUBMED   17047026
REFERENCE   8  (bases 1 to 4864)
  AUTHORS   Wishart,M.J. and Dixon,J.E.
  TITLE     The archetype STYX/dead-phosphatase complexes with a spermatid
            mRNA-binding protein and is essential for normal sperm production
  JOURNAL   Proc Natl Acad Sci U S A 99 (4), 2112-2117 (2002)
   PUBMED   11842224
REFERENCE   9  (bases 1 to 4864)
  AUTHORS   Dayton,M.A. and Knobloch,T.J.
  TITLE     Multiple phosphotyrosine phosphatase mRNAs are expressed in the
            human lung fibroblast cell line WI-38
  JOURNAL   Recept Signal Transduct 7 (4), 241-256 (1997)
   PUBMED   9633825
REFERENCE   10 (bases 1 to 4864)
  AUTHORS   Wishart,M.J., Denu,J.M., Williams,J.A. and Dixon,J.E.
  TITLE     A single mutation converts a novel phosphotyrosine binding domain
            into a dual-specificity phosphatase
  JOURNAL   J Biol Chem 270 (45), 26782-26785 (1995)
   PUBMED   7592916
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB498442.1, AK098195.1,
            BC020265.2, AL139317.5 and BQ772275.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_145251.3.
            
            Summary: The protein encoded by this gene is a pseudophosphatase,
            able to bind potential substrates but lacking an active catalytic
            loop. The encoded protein may be involved in spermiogenesis. Two
            transcript variants encoding the same protein have been found for
            these genes. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) is the more frequently
            occurring transcript. Variants 1 and 2 both encode the same
            protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.277347.1,
                                           SRR1803617.224983.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354586.5/ ENSP00000346599.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                DB498442.1         5-20
            17-161              AK098195.1         1-145
            162-907             BC020265.2         114-859
            908-4309            AL139317.5         28917-32318
            4310-4864           BQ772275.1         15-569              c
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q22.1"
     gene            1..4864
                     /gene="STYX"
                     /note="serine/threonine/tyrosine interacting protein"
                     /db_xref="GeneID:6815"
                     /db_xref="HGNC:HGNC:11447"
                     /db_xref="MIM:615814"
     exon            1..366
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     CDS             310..981
                     /gene="STYX"
                     /note="protein tyrosine phosphatase-like protein; inactive
                     tyrosine-protein phosphatase STYX;
                     phosphoserine/threonine/tyrosine interaction protein"
                     /codon_start=1
                     /product="serine/threonine/tyrosine-interacting protein"
                     /protein_id="NP_660294.1"
                     /db_xref="CCDS:CCDS9711.1"
                     /db_xref="GeneID:6815"
                     /db_xref="HGNC:HGNC:11447"
                     /db_xref="MIM:615814"
                     /translation="MEDVKLEFPSLPQCKEDAEEWTYPMRREMQEILPGLFLGPYSSA
                     MKSKLPVLQKHGITHIICIRQNIEANFIKPNFQQLFRYLVLDIADNPVENIIRFFPMT
                     KEFIDGSLQMGGKVLVHGNAGISRSAAFVIAYIMETFGMKYRDAFAYVQERRFCINPN
                     AGFVHQLQEYEAIYLAKLTIQMMSPLQIERSLSVHSGTTGSLKRTHEEEDDFGTMQVA
                     TAQNG"
     exon            367..399
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            400..453
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            454..551
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            552..612
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            613..649
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            650..689
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            690..740
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            741..813
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            814..907
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     exon            908..4864
                     /gene="STYX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4801..4806
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STYX"
                     /note="hexamer: ATTAAA"
     polyA_site      4819
                     /gene="STYX"
                     /note="major polyA site"
     regulatory      4833..4838
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STYX"
                     /note="hexamer: ATTAAA"
     polyA_site      4864
                     /gene="STYX"
ORIGIN      
        1 ggttgtcggg ctggttcctg tgctggatcc tgggcggcct gaggggtacg gagactctgg
       61 gggagggaga cggcagcggc atggcggccg ggtgtaagac gcccgaccct cctcttccct
      121 gtcttcgccg ccgccgctgc tggagtcact gggaccctgt agtctgcgtg tgttagttgt
      181 aatcccgccg ccctcctgtc agccctccgc tccgccggcc ctccttcctt ccgccgccgc
      241 agccagcccg agggtcggcc ggctgtgtaa cactctccca ccccacccac cagcccgcgg
      301 gccagcacca tggaggacgt gaagctggag ttcccttccc ttccacagtg caaggaagac
      361 gccgaggagt ggacctaccc tatgagacga gagatgcagg aaattttacc tggattgttc
      421 ttaggcccat attcatctgc tatgaaaagc aagctacctg tactacagaa acatggaata
      481 acccatataa tatgcatacg acaaaatatt gaagcaaact ttattaaacc aaactttcag
      541 cagttattta gatatttagt cctggatatt gcagataatc cagttgaaaa tataatacgt
      601 tttttcccta tgactaagga atttattgat gggagcttac aaatgggagg aaaagttctt
      661 gtgcatggaa atgcagggat ctccagaagt gcagcctttg ttattgcata cattatggaa
      721 acatttggaa tgaagtacag agatgctttt gcttatgttc aagaaagaag attttgtatt
      781 aatcctaatg ctggatttgt ccatcaactt caggaatatg aagccatcta cctagcaaaa
      841 ttaacaatac agatgatgtc accactccag atagaaaggt cattatctgt tcattctggt
      901 accacaggca gtttgaagag aacacatgaa gaagaggatg attttggaac catgcaagtg
      961 gcgactgcac agaatggctg acttgaagag caacatcata gagtgtgaat ttctatttgg
     1021 gaaggagaaa atacaagaga aaattataat gtaaaatggt aaaaacataa gtagtttttt
     1081 tttcaattac atgttgcttc cagacatact tctctgcaac ttgttgagca acattttaag
     1141 atgttggact tctgcaatag atgacactga tggttttact ccttttttta aaaacacatg
     1201 cgcgcgcaca cacacatgct ttacaagttt tattataaac caagaatttt ggacttgcaa
     1261 agaggtatta ttgcaataat gcacttttca tacttgaaat ttatttgtat gatataaagt
     1321 tattacttta aacaaaatgc aagtatgggg ggattgttta taaagtttgg gtaatttata
     1381 acaaaatttg ctaaggtttg ctaaaaattc atttttctgt tctatatatt acatttttaa
     1441 cataatttta cagttcaatt ttatgatgga gcctcttaca gaaacattaa caaaatgcag
     1501 gaatctgcca catttctttt ttagtataac ttaatagctt aattaccatt ttatttttta
     1561 tacttcttcc attattaatc tttaaatcat gatcctaatt agctgtcctt actttaactt
     1621 gatctaatta ttgcttcctt tcttattact ttcctaattt ttctatattt taaaaactac
     1681 agtttccatg ataaaaggaa aacgttttga tttatagtac caagtgctta aacacaagga
     1741 tagtgttaga ttttcgagtg actttccttt ttgcattttt tggcagtaaa agccaaacgt
     1801 tgtatttgtt cttttcagag ttgtccagcc cttttttcct ttgtccaaaa tgattctaaa
     1861 tagaatctaa taaaccaatg tagcattatt tttttctaaa tgaagcccca aaaaagaaaa
     1921 gtgccttgca tcatttaaaa aaaataatta aatcctcatg gcctctaaat tagtatgtag
     1981 aacactgaaa agttcttaac atttttgtgt aatttccttt ctttttaaac cataaattag
     2041 tttaaactga aagtacgagg ctggaagaaa tattagtaaa ttatttggaa tatagaatgt
     2101 ttactctttc tttttatgtt gtcttaatga ttctgtgaga ttgttccggc tcaaacagaa
     2161 gcttttcttt ggggaaggtg atttgtggga gactagtgta ttttaaatta gcattttaat
     2221 ccattcttga cattcagtta gtccagatct gccccataat ttgctttagt aaagtcactt
     2281 tatggatttt tggctatgtt ttagtttgtg tgtataaaag ttctaagaaa acatttttgc
     2341 tattttaagt atgtaaggga agagaggagt gtttttaact ttttatagtt gatgacttta
     2401 gggtagcaca aacaaaactc ctttgtatct aacttttctc aatcctctct tgaggtgctt
     2461 tactaatggg aatgatttct gtatgttccc ttggtaccaa gaggtactat gcaaagtaac
     2521 ctattacacc aagttacttg ctttgctttc ctctctatga tgtgataata cagtaaaagc
     2581 tttcttaccc agcatagtgg gagagtggag attaattaaa attgttaatt aagagttaat
     2641 tcctattgac ccaggtgata tttctcttct gatttccctc cccttccctt ctcttatctt
     2701 accactgtga aaacagcata ttgttaatct cgttgtcgtc cagtattctg ctttgtgatt
     2761 aggtcttttg atgtacagtg gtctagtgga gtcaagattc gcattgggtt ttctaaaatt
     2821 ccagttgata aaagttccag ataacacagc tttcctgtat atagatcact attgggcagg
     2881 tcagcaaaga tctcttacag tgtaataata atctatgatg cttcatttag cagaaactct
     2941 gcttaaaaga atcttcataa tagtaagttt aggttttaaa aacttgtttc ataaatatac
     3001 atatatcctc tctagtagtc tggccaaaag aacagatttt gttattgata atttgtagct
     3061 ggtaattttc cacattttct atccactgta atttttatgt tgtcactgaa gtgcctgccc
     3121 agtactgtat attacagtct ctcacaaaca ctgggaaaag ggactgtcat catcttgagt
     3181 actctgtgtg tatatatata tatatagata gatagatttt tttttttttt tgagacagag
     3241 tctctaatgt cacccaggct ggagtacagt ggcacaatct tggctcactg caacctccac
     3301 ctcctgggtt caagtgattt tcctgcctca gcctcccaag tagctggggt tagaggcaca
     3361 tgccaccatg cctggctaat ttttgtagtt ttagtagaga tggggtttca ccatgttggc
     3421 caggctggtc tcaaactcct gacctcaagt gatccaccca cctcggcctc ccaaagtgct
     3481 gggattacag gcgtgagcca ctgcgcctgg ctgagtacaa tattaatgta gacaaaccat
     3541 gaagtttatt atttcatata agaacattac aggtttgttt tttcttgcat gtctgtccac
     3601 ctaatgttta agtagttctg gtagctcttc ctattcttta ttctatttga ttccatttct
     3661 gtgattcttt tattaccact gatgttttgt gatagttaac tatgataaat ttaactgatc
     3721 atgatttatc ttctagagta tttaaataat gtatgagtga ccacccaatt ccaacattaa
     3781 aagtgtaatc tgggcccata atttatagtg aaattgtatc aaaacatagg gaaactgtat
     3841 tactgtccat tttgaaaata tgaaacttga gtattgaaaa tattcaaaca tggaatggca
     3901 gtattctaat ttcagttagt tggttcatgt taatttctta cctgttagat gtttaaactg
     3961 cagtgacctt tacttgtatc tactctgtgg tggaaatgtt aaaccatgat agcttttgct
     4021 accaactcaa ccacttaact tttagagcag ttttggggag agtttatgct tcatctgagt
     4081 ttagaagtaa tgtcagaaaa tgttaagcat gtctgtatta agaaaatata aggtttctaa
     4141 ttgtcttatt aatatggtaa ttcaagtgaa ttagaaatat ttaactgcaa tcttgaatta
     4201 taaagttgag atatatatat atatgtatca agatctcaac ttgatgtaaa gtaaatgagc
     4261 agttacctgg cggatttttt tttttttaaa taactgattt aatccataat cccataacaa
     4321 acatagcttc acctcagtat tttctttctt tctttgttca acagtgctcc gataagggaa
     4381 tgctagaaaa tagatgagaa gtactgaaag accttttttt ttaattgatt agaaaagtaa
     4441 gtctctaggg tctttgaatg ctggaatttt tttttttttt tttgtctttc ccatctgtgg
     4501 cagctaaaac aaaaatcact caaaatattc aggtttacat gttagctctc tctcataggg
     4561 agctgccata cctcacagtt caaagtgtat tctatagatc agtaacatta tactgacatg
     4621 taattgcaat ttactatgca gcaaaaatga ttcaagaaga aaaataacct acagtgtctg
     4681 tatacctttg tatacacaat tgcttaagtt actctgcttt taacatttgt acttggataa
     4741 aatgcttatg tctgtatagg aatgtcacag tgcaagatgc tgctagccca ggcacaaagt
     4801 attaaaatta ttttgtgaag attggtggtt gtattaaaac tgctgtgcca ttatacctcc
     4861 aaaa
//
