LOCUS       NM_001320752            6622 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens steroid sulfatase (STS), transcript variant 4, mRNA.
ACCESSION   NM_001320752 XM_005274511
VERSION     NM_001320752.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Park,J., Cho,Y.G., Kim,J.K. and Kim,H.H.
  TITLE     STS and PUDP Deletion Identified by Targeted Panel Sequencing with
            CNV Analysis in X-Linked Ichthyosis: A Case Report and Literature
            Review
  JOURNAL   Genes (Basel) 14 (10), 1925 (2023)
   PUBMED   37895274
  REMARK    GeneRIF: STS and PUDP Deletion Identified by Targeted Panel
            Sequencing with CNV Analysis in X-Linked Ichthyosis: A Case Report
            and Literature Review.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Khan,N., Shah,K., Fozia,F., Khan,S.A., Muhammad,N., Nasir,A.,
            Ahmad,I., Rehman,Z.U., Jan,A., Muhammad,N. and Khan,S.
  TITLE     Biallelic mutations in FLG, TGM1, and STS genes segregated with
            different types of ichthyoses in eight families of Pakistani origin
  JOURNAL   Int J Dermatol 62 (5), 672-680 (2023)
   PUBMED   36789964
  REMARK    GeneRIF: Biallelic mutations in FLG, TGM1, and STS genes segregated
            with different types of ichthyoses in eight families of Pakistani
            origin.
REFERENCE   3  (bases 1 to 6622)
  AUTHORS   Ghosh,D.
  TITLE     Structure of human placental steroid sulfatase at 2.0 angstrom
            resolution: Catalysis, quaternary association, and a secondary
            ligand site
  JOURNAL   J Steroid Biochem Mol Biol 227, 106228 (2023)
   PUBMED   36427797
  REMARK    GeneRIF: Structure of human placental steroid sulfatase at 2.0
            angstrom resolution: Catalysis, quaternary association, and a
            secondary ligand site.
REFERENCE   4  (bases 1 to 6622)
  AUTHORS   Baek,H.S., Kwon,T.U., Shin,S., Kwon,Y.J. and Chun,Y.J.
  TITLE     Steroid sulfatase deficiency causes cellular senescence and
            abnormal differentiation by inducing Yippee-like 3 expression in
            human keratinocytes
  JOURNAL   Sci Rep 11 (1), 20867 (2021)
   PUBMED   34675221
  REMARK    GeneRIF: Steroid sulfatase deficiency causes cellular senescence
            and abnormal differentiation by inducing Yippee-like 3 expression
            in human keratinocytes.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6622)
  AUTHORS   Nardi,A., Pomari,E., Zambon,D., Belvedere,P., Colombo,L. and Dalla
            Valle,L.
  TITLE     Transcriptional control of human steroid sulfatase
  JOURNAL   J Steroid Biochem Mol Biol 115 (1-2), 68-74 (2009)
   PUBMED   19429462
  REMARK    GeneRIF: two new splicing patterns found in prostate and ovary
            cells; report gene expression analysis for the simultaneous
            detection, in qualitative and/or semi-quantitative terms, of the
            transcription patterns of STS in different tissues
REFERENCE   6  (bases 1 to 6622)
  AUTHORS   Dalla Valle,L., Toffolo,V., Nardi,A., Fiore,C., Armanini,D.,
            Belvedere,P. and Colombo,L.
  TITLE     The expression of the human steroid sulfatase-encoding gene is
            driven by alternative first exons
  JOURNAL   J Steroid Biochem Mol Biol 107 (1-2), 22-29 (2007)
   PUBMED   17601726
  REMARK    GeneRIF: the regulation of STS transcription appears to be more
            complex than previously thought, suggesting that this enzyme plays
            a substantial role in intercellular integration.
REFERENCE   7  (bases 1 to 6622)
  AUTHORS   Zaichuk,T., Ivancic,D., Scholtens,D., Schiller,C. and Khan,S.A.
  TITLE     Tissue-specific transcripts of human steroid sulfatase are under
            control of estrogen signaling pathways in breast carcinoma
  JOURNAL   J Steroid Biochem Mol Biol 105 (1-5), 76-84 (2007)
   PUBMED   17596930
REFERENCE   8  (bases 1 to 6622)
  AUTHORS   Basler,E., Grompe,M., Parenti,G., Yates,J. and Ballabio,A.
  TITLE     Identification of point mutations in the steroid sulfatase gene of
            three patients with X-linked ichthyosis
  JOURNAL   Am J Hum Genet 50 (3), 483-491 (1992)
   PUBMED   1539590
REFERENCE   9  (bases 1 to 6622)
  AUTHORS   Shankaran,R., Ameen,M., Daniel,W.L., Davidson,R.G. and Chang,P.L.
  TITLE     Characterization of arylsulfatase C isozymes from human liver and
            placenta
  JOURNAL   Biochim Biophys Acta 1078 (2), 251-257 (1991)
   PUBMED   2065092
REFERENCE   10 (bases 1 to 6622)
  AUTHORS   Stein,C., Hille,A., Seidel,J., Rijnbout,S., Waheed,A., Schmidt,B.,
            Geuze,H. and von Figura,K.
  TITLE     Cloning and expression of human steroid-sulfatase. Membrane
            topology, glycosylation, and subcellular distribution in BHK-21
            cells
  JOURNAL   J Biol Chem 264 (23), 13865-13872 (1989)
   PUBMED   2668275
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC073583.19, DA726770.1,
            DA855321.1, AC005704.1, BX390437.1, AC112651.4 and BX119415.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 23, 2019 this sequence version replaced NM_001320752.1.
            
            Summary: This gene encodes a multi-pass membrane protein that is
            localized to the endoplasmic reticulum. It belongs to the sulfatase
            family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which
            serve as metabolic precursors for estrogens, androgens, and
            cholesterol. Mutations in this gene are associated with X-linked
            ichthyosis (XLI). Alternatively spliced transcript variants
            resulting from the use of different promoters have been described
            for this gene (PMID:17601726). [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (4) uses an alternate promoter and
            differs in the 5' UTR, compared to variant 1. Variants 1 and 4
            encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.2223254.1,
                                           SRR14038191.3308154.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000674429.1/
                                                ENSP00000501534.1
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-300               AC073583.19        118035-118334
            301-867             DA726770.1         1-567
            868-888             DA855321.1         134-154
            889-895             AC005704.1         5364-5370           c
            896-1228            DA855321.1         162-494
            1229-1311           AC005704.1         4948-5030           c
            1312-1324           DA855321.1         578-590
            1325-1773           BX390437.1         181-629
            1774-1864           AC112651.4         71318-71408
            1865-1887           BX390437.1         721-743
            1888-2187           BX119415.1         389-688
            2188-6622           AC112651.4         87512-91946
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.31"
     gene            1..6622
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /note="steroid sulfatase"
                     /db_xref="GeneID:412"
                     /db_xref="HGNC:HGNC:11425"
                     /db_xref="MIM:300747"
     exon            1..372
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            373..501
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            502..642
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     CDS             506..2242
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /EC_number="3.1.6.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 4; steryl-sulfatase; estrone sulfatase;
                     arylsulfatase C; steryl-sulfate sulfohydrolase; steroid
                     sulfatase (microsomal), isozyme S"
                     /codon_start=1
                     /product="steryl-sulfatase isoform 1 precursor"
                     /protein_id="NP_001307681.2"
                     /db_xref="CCDS:CCDS14127.2"
                     /db_xref="GeneID:412"
                     /db_xref="HGNC:HGNC:11425"
                     /db_xref="MIM:300747"
                     /translation="MKIPFLLLFFLWEAESHAASRPNIILVMADDLGIGDPGCYGNKT
                     IRTPNIDRLASGGVKLTQHLAASPLCTPSRAAFMTGRYPVRSGMASWSRTGVFLFTAS
                     SGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYGISLT
                     NLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLA
                     ALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTETPFL
                     LVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYF
                     TSDQGAHVEEVSSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEP
                     TSNMDIFPTVAKLAGAPLPEDRIIDGRDLMPLLEGKSQRSDHEFLFHYCNAYLNAVRW
                     HPQNSTSIWKAFFFTPNFNPVGSNGCFATHVCFCFGSYVTHHDPPLLFDISKDPRERN
                     PLTPASEPRFYEILKVMQEAADRHTQTLPEVPDQFSWNNFLWKPWLQLCCPSTGLSCQ
                     CDREKQDKRLSR"
     exon            643..764
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            765..887
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1311
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1448
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1586
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1746
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1868
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..6622
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6599..6604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /note="hexamer: AATAAA"
     polyA_site      6622
                     /gene="STS"
                     /gene_synonym="ARSC; ARSC1; ASC; ES; SSDD; XLI"
                     /note="major polyA site"
ORIGIN      
        1 gctctgagga gaggacgcgg agccgcgcgc ccgcccggtg gaccccaggg actgctccca
       61 cgtgctccgg ccccacgcgc gggccgccga ccggaagcgg gtgctggggc cgcgtagacg
      121 ccgcggccac gcgcgcccgc cagcccggac atgggcccgc gggcgctcct ggccgccgcc
      181 cgacttcggg gccagccggg ggcagagcgc gcgggagccc gagcgtccct gcatgaacac
      241 cgccccgccg cggcccccag gccgtgacgt accccgcgcc gaccgtcccc acgcccacac
      301 aagaccgccc ttactggagg cggcggctgc acactaccca cccagaagaa gtccgtccat
      361 gtcaaagatg aggaagagcc cgatgccctt ggtttgactc tactaagagc ccctcagttg
      421 ctgaacctgc acacagtcat ctcagtaagt taagatcttc ctgaggacaa tggcgcaaga
      481 tcgtcttcag ctgttcatag cgaagatgaa gatccctttc ctcctactgt tctttctgtg
      541 ggaagccgag agccacgcag catcaaggcc gaacatcatc ctggtgatgg ctgacgacct
      601 cggcattgga gatcctgggt gctatgggaa caaaactatc aggactccca atatcgaccg
      661 gttggccagt gggggagtga aactcactca gcacctggca gcatcaccgc tgtgcacacc
      721 aagcagggca gccttcatga ctggccggta ccctgtccga tcaggaatgg catcttggtc
      781 ccgcactgga gttttcctct tcacagcctc ttcgggagga cttcccaccg atgagattac
      841 ctttgctaag cttctgaagg atcaaggtta ttcaacagca ctgataggga aatggcacct
      901 tgggatgagc tgtcacagca agactgactt ctgtcaccac cctttacatc acggcttcaa
      961 ttatttctat gggatctctt tgaccaatct gagagactgc aagcccggag agggcagtgt
     1021 cttcaccacg ggcttcaaga ggctggtctt cctccccctg cagatcgtcg gggtcaccct
     1081 ccttaccctt gctgcactca attgtctggg gctactccac gtgcctctag gcgttttttt
     1141 cagccttctc ttcctagcag ccctaatcct gacccttttc ttgggcttcc ttcattactt
     1201 ccggcccctg aactgcttca tgatgaggaa ctacgagatc attcagcagc ccatgtccta
     1261 tgacaatctc acccagaggc taacggtgga ggcggcccag ttcatacagc ggaacactga
     1321 gactccgttc ctgcttgtct tgtcctacct ccacgtgcac acagccctgt tctccagcaa
     1381 agactttgct ggcaaaagtc aacacggagt ctacggggat gctgttgagg aaatggactg
     1441 gagtgtgggg cagatcttga accttctgga tgagctgaga ttggctaatg ataccctcat
     1501 ctacttcaca tcggaccagg gagcacatgt agaagaagtg tcttccaaag gagaaattca
     1561 tggcggaagt aatgggatct ataaaggagg aaaagcaaac aactgggaag gaggtatccg
     1621 ggttccaggc atccttcgtt ggcccagggt gatacaggct ggccagaaga ttgatgagcc
     1681 cactagcaac atggacatat ttcctacagt agccaagctg gctggagctc ccttgcctga
     1741 ggacaggatc attgatggac gtgatctgat gcccctgctt gaaggaaaaa gccaacgctc
     1801 cgatcatgag tttctcttcc attactgcaa cgcctactta aatgctgtgc gctggcaccc
     1861 tcagaacagc acatccatct ggaaggcctt tttcttcacc cccaacttca accccgtggg
     1921 ttccaacgga tgctttgcca cacacgtgtg cttctgtttc gggagttatg tcacccatca
     1981 cgacccacct ttactctttg atatttccaa agatcccaga gagagaaacc cactaactcc
     2041 agcatccgag ccccggtttt atgaaatcct caaagtcatg caggaagctg cggacagaca
     2101 cacccagacc ctgccagagg tgcccgatca gttttcatgg aacaactttc tttggaagcc
     2161 ctggcttcag ctgtgctgtc cttccaccgg cctgtcttgc cagtgtgata gagaaaaaca
     2221 ggataagaga ctgagccgct agcagcgcct ggggaccaga cagacgcatg tggcaaagct
     2281 caccatcttc actacaaaca cgcctgagag tggcactggg gaaacataac tccatctaca
     2341 ccttggattt ggactgattc tccattttat cacctgaagg cttgggccag agctcaacag
     2401 ctactcaact ggaggggtga gggggataag gtctgtagta tacagacagg aagatggtag
     2461 gtttatgcct tctgtggcca gagtcttgga ctcatggaaa tagaatgaat agaggggcat
     2521 tcacaaggca caccagtgca agcagatgac aaaaaggtgc agaaggcaat cttaaaacag
     2581 aaaggtgcag gaggtacctt aactcacccc tcagcaaata cctatgtcaa cagtataagt
     2641 taccatttac tctataatct gcagtgatgc aataaccagc ataataaaaa ggcaatcaca
     2701 taaaaaagag tttagtcgtc taaacataag taactttaag gtgaatgaaa gatcttcttt
     2761 aggaataata gatgatggta agttccactt tggttattgg aaggcaagtc attattactg
     2821 gtattagtta aaacacatat caaatgcttg ctcttcatca tatatatagt tatgcataca
     2881 tacacacaca cacatacagt atattctttc ctcaaaaggg ttaagatgtc taaaataggg
     2941 acctagaagc ttaacactat ttaagtaaat acagtagaag ctcacaaata gatttctttg
     3001 cacaatgatt ttttgcaaaa ttttacagta ataataatcc caaggcaaat ctctcctgaa
     3061 ctgctttcca ttccataatt tgtagtataa ttcttggatt ccactgtttt ctttggggaa
     3121 tggaagttct gaattaaaag cccactgtgg agatgctgtg gttcatggaa tctcttccag
     3181 tgtaattcag aatcattggc ctagaaagtc tctgatattt ggaggggaac aaaaatcact
     3241 cacaagcaat ccatgatcta tacacataag cataatttcc tttagttcta gttagtcatc
     3301 agagaacagt catgtatgca agttttgtga ctgagaaatt tctgtgcttc caatccacaa
     3361 tgagatgcat gattttgttt tcatcccatt tcccccaagc ccctgtaaat cagggaaaat
     3421 gcgcaactga tcgcctagga gagggcctcg tagtggcaca gctggagata gtttcaaagt
     3481 ctaaaccacc agcccatcct gaggaaagcc tcctatggaa tgtaaagtgc aatcatttct
     3541 tcagatataa gactttcccc aacaatgtga ttggattcct ttatggcaaa atcgagagaa
     3601 gctgccatcc acctgcttat gcatttatct cttttgtgga cttgtctgac caccttctat
     3661 ttgcccagag tttgctcaat tccaagacag tgcccatgaa tgggacacct gtaatgtaac
     3721 ccacacagcg gtttgcagag aatgttagcc atgacttggg ctttctgaaa gttggctata
     3781 atttctctat ccctacccac aaccctggga agttggagca agaggggcat actattgggc
     3841 tgggaggatt tgacagcatt tccccagttg ccctttaagt tcttctattt caaacgttaa
     3901 ttttgcttct ctttctaaaa aaaaaaaaaa aaaagaaaga gaagaaagaa gtgattccta
     3961 ccccctacct ccagagttgt tgaaagctga aaagcataca agattcttcc ttttaacttg
     4021 gatttctcgt tccagaaatt gtgggataat ctgtattctt gctttagaaa acattcttag
     4081 agagggtact agcttactga tgatgtgtta ggattgctac tgatgctgtc atgtggaaac
     4141 tatttaaagg cactattata aatttatcct ataagatgac aatgtttact caaagtctaa
     4201 catattcaat gcaagtaaga ctttctgaaa acacttgatg atgtggaaat gctgcaggat
     4261 taaataactt gaagagcctt tatagattat atgaatgcct atttgtgtct agaaccagtt
     4321 atttaacctg taaaatgtca atagcaaatg aaggatgaag tatatctcta gatgcaaata
     4381 cattgagttt aaaagtgcct caaaataatt gagatcacat ttcaggacat ttggaaatca
     4441 ggtcgatttg tggtaactgt agtcatctta aatttcaaac catttaccat ctgaaagttt
     4501 tgatttgaat gtaaaacagg aaattggaat tcctttgtcc aggagaaacc tcacaaacct
     4561 tctttaaggc atagttttgt tgtttgtttg cttgtttgtt gcaggctgta aggcatggct
     4621 gcttgtttac aaagcatctc attcatatta cctgtggagt tgcatatcca aaccttagtg
     4681 agttttgaag ctttaagcaa attcttttaa aaaattcttg tatttctagc attactagat
     4741 attaaaagtt aagcaaatag attaatgacg tatacatagg catcatttca caaggtcagt
     4801 aatgctgcag gaaaagcaaa attgcaatct acgtatctat ggtactaagg aagtcctgtt
     4861 tttcaaaaat ggaagcccac ttctcagatt tttctgaagg gcatacaatg aaaagtgaag
     4921 gggaaacaca cacacacaaa aaaacaagta tttggcttgt cacaggaatc tgattgcatt
     4981 aagtgaaagg attatttaga atatgttaat gcaaagctaa aataaaattt tccttggcaa
     5041 ttaaaaatgc tgtgcgctaa taccctgctt tctatcgtga ctcaattcaa caatgtgggg
     5101 aatgtttact acatttccaa cttgatgtca agcaatgggg aatacaagtt ccagttctgc
     5161 aaagattcgt caactttctt agctcaagag agaggctgag aaatgcagag aagaataaga
     5221 cataaaatag ctccgacctc catgatccga gagtgggaaa aggcccgatt attacccata
     5281 aggcacactc tctaaggcct tttaaggggc ctacaaaaat gttttatttt ataatcagaa
     5341 gaaaaggaaa tgaacattgg ggattgaaaa tcatattggt atttgcacca acatagtcat
     5401 aaaatagtat gttaatatgt ttttacttta tatatttata tattaaaata tatttaatat
     5461 gttttgcctt tgtggcccat gaaagtctta ctgggccctg gggaaggtat cctaccctgg
     5521 tgaagcagct gctttgctct acaaatacct ggggcagaaa tttgatttga aaagtattat
     5581 tctctcttct ctttgtttca actggattcc tttggaaaac caaactagta tcagaacaaa
     5641 ccccgaaaca gtaagaaatt ggagtgagaa gggcatggta ttgggactag gatcggctct
     5701 cattcgatcg agctattctc ttaaaatgac aaaaagtgtc cataaagagg ctgctggaga
     5761 gtgcgtggcc atagggagcc gacatgcccg ggaggaaagg tgttgattac atggatactt
     5821 ctaaaagcta aagccttgtt gccttctctt taatgcctag agaatgggat gtgtgatgca
     5881 aatgctcaaa acctcttaaa tcatagctgt ctgacctcta cggacctcac atccatctga
     5941 ggcttcatgg acaaagattc tccacttggc caaactttag ccaagctcct caaccttctc
     6001 ccaggcccaa tctgggcact tccttgtaaa atctagtttt ggcaagaagt ctattaggtc
     6061 agtttagcaa gaacacctaa cccccccata tctgttcaac ctcagtatct gatcaggctc
     6121 ctcagcctcc accatccccc aggtgatgtc tggtcaactg gcctgccttc agctagaatc
     6181 ctgttaggtc ggtttagatg aatgctccct gatatttcct cttggtaatc ttccatccac
     6241 tgcccctgac cctgttccct gtctataaat ccccagtttt ccatggtgta ttcagagctg
     6301 agtccagtct ctctccccta ctacaagtcc ccattgctgt ggtccccgta cctgtcatga
     6361 tggtcctaaa taaagtctta ctgtgcttta ataggtagca ttgaaaaatt tttttctttg
     6421 acatcattca tgacaacatg aaacctattg ggacagcatg actgtgcagg gtctttagag
     6481 ctcagctttc tgaggccctg agcattcttg gttttccgac atcgtgaacc tgttctgtgt
     6541 tgcaagatat cactcaagcc agtgttgctt aataccatct ctttgtgtaa tagatctgaa
     6601 taaagtaatt gtaatacacc aa
//
