LOCUS       NM_001003787            2166 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens STE20 related adaptor alpha (STRADA), transcript
            variant 1, mRNA.
ACCESSION   NM_001003787
VERSION     NM_001003787.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Kim,M., Park,J., Bouhaddou,M., Kim,K., Rojc,A., Modak,M.,
            Soucheray,M., McGregor,M.J., O'Leary,P., Wolf,D., Stevenson,E.,
            Foo,T.K., Mitchell,D., Herrington,K.A., Munoz,D.P., Tutuncuoglu,B.,
            Chen,K.H., Zheng,F., Kreisberg,J.F., Diolaiti,M.E., Gordan,J.D.,
            Coppe,J.P., Swaney,D.L., Xia,B., van 't Veer,L., Ashworth,A.,
            Ideker,T. and Krogan,N.J.
  TITLE     A protein interaction landscape of breast cancer
  JOURNAL   Science 374 (6563), eabf3066 (2021)
   PUBMED   34591612
REFERENCE   3  (bases 1 to 2166)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2166)
  AUTHORS   Smith,R.H.B., Khan,Z.M., Ung,P.M., Scopton,A.P., Silber,L.,
            Mack,S.M., Real,A.M., Schlessinger,A. and Dar,A.C.
  TITLE     Type II Binders Targeting the 'GLR-Out' Conformation of the
            Pseudokinase STRADalpha
  JOURNAL   Biochemistry 60 (4), 289-302 (2021)
   PUBMED   33440120
  REMARK    GeneRIF: Type II Binders Targeting the ''GLR-Out'' Conformation of
            the Pseudokinase STRADalpha.
REFERENCE   5  (bases 1 to 2166)
  AUTHORS   Alsaif,H.S., Khashab,H.Y.E.L. and Alkuraya,F.S.
  TITLE     Two further cases of polyhydramnios, megalencephaly, and
            symptomatic epilepsy syndrome, caused by a truncating variant in
            STRADA
  JOURNAL   Am J Med Genet A 185 (2), 604-607 (2021)
   PUBMED   33247513
  REMARK    GeneRIF: Two further cases of polyhydramnios, megalencephaly, and
            symptomatic epilepsy syndrome, caused by a truncating variant in
            STRADA.
REFERENCE   6  (bases 1 to 2166)
  AUTHORS   Brajenovic,M., Joberty,G., Kuster,B., Bouwmeester,T. and Drewes,G.
  TITLE     Comprehensive proteomic analysis of human Par protein complexes
            reveals an interconnected protein network
  JOURNAL   J Biol Chem 279 (13), 12804-12811 (2004)
   PUBMED   14676191
REFERENCE   7  (bases 1 to 2166)
  AUTHORS   Milburn,C.C., Boudeau,J., Deak,M., Alessi,D.R. and van Aalten,D.M.
  TITLE     Crystal structure of MO25 alpha in complex with the C terminus of
            the pseudo kinase STE20-related adaptor
  JOURNAL   Nat Struct Mol Biol 11 (2), 193-200 (2004)
   PUBMED   14730349
REFERENCE   8  (bases 1 to 2166)
  AUTHORS   Boudeau,J., Baas,A.F., Deak,M., Morrice,N.A., Kieloch,A.,
            Schutkowski,M., Prescott,A.R., Clevers,H.C. and Alessi,D.R.
  TITLE     MO25alpha/beta interact with STRADalpha/beta enhancing their
            ability to bind, activate and localize LKB1 in the cytoplasm
  JOURNAL   EMBO J 22 (19), 5102-5114 (2003)
   PUBMED   14517248
REFERENCE   9  (bases 1 to 2166)
  AUTHORS   Baas,A.F., Boudeau,J., Sapkota,G.P., Smit,L., Medema,R.,
            Morrice,N.A., Alessi,D.R. and Clevers,H.C.
  TITLE     Activation of the tumour suppressor kinase LKB1 by the STE20-like
            pseudokinase STRAD
  JOURNAL   EMBO J 22 (12), 3062-3072 (2003)
   PUBMED   12805220
  REMARK    GeneRIF: Identification and characterization of an LKB1-specific
            adaptor protein and substrate, STRAD. Results imply that STRAD
            plays a key role in regulating the tumor suppressor activities of
            LKB1.
REFERENCE   10 (bases 1 to 2166)
  AUTHORS   Scanlan,M.J., Gout,I., Gordon,C.M., Williamson,B., Stockert,E.,
            Gure,A.O., Jager,D., Chen,Y.T., Mackay,A., O'Hare,M.J. and Old,L.J.
  TITLE     Humoral immunity to human breast cancer: antigen definition and
            quantitative analysis of mRNA expression
  JOURNAL   Cancer Immun 1, 4 (2001)
   PUBMED   12747765
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY290821.2, BK001542.1,
            AL832407.1 and BQ682133.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001003787.3.
            
            Summary: The protein encoded by this gene contains a STE20-like
            kinase domain, but lacks several residues that are critical for
            catalytic activity, so it is termed a 'pseudokinase'. The protein
            forms a heterotrimeric complex with serine/threonine kinase 11
            (STK11, also known as LKB1) and the scaffolding protein calcium
            binding protein 39 (CAB39, also known as MO25). The protein
            activates STK11 leading to the phosphorylation of both proteins and
            excluding STK11 from the nucleus. The protein is necessary for
            STK11-induced G1 cell cycle arrest. A mutation in this gene has
            been shown to result in polyhydramnios, megalencephaly, and
            symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            Additional transcript variants have been described but their
            full-length nature is not known. [provided by RefSeq, Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.671002.1,
                                           SRR3476690.1104067.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336174.12/ ENSP00000336655.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AY290821.2         172-257
            87-1146             BK001542.1         97-1156
            1147-2163           AL832407.1         1102-2118
            2164-2166           BQ682133.1         605-607
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.3"
     gene            1..2166
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /note="STE20 related adaptor alpha"
                     /db_xref="GeneID:92335"
                     /db_xref="HGNC:HGNC:30172"
                     /db_xref="MIM:608626"
     exon            1..59
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            60..139
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..1399
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /note="isoform 1 is encoded by transcript variant 1;
                     STE20-like pseudokinase; STE20-related kinase adapter
                     protein alpha; protein kinase LYK5; serologically defined
                     breast cancer antigen NY-BR-96; STE20-related kinase
                     adaptor alpha"
                     /codon_start=1
                     /product="STE20-related kinase adapter protein alpha
                     isoform 1"
                     /protein_id="NP_001003787.1"
                     /db_xref="CCDS:CCDS32703.1"
                     /db_xref="GeneID:92335"
                     /db_xref="HGNC:HGNC:30172"
                     /db_xref="MIM:608626"
                     /translation="MSFLVSKPERIRRWVSEKFIVEGLRDLELFGEQPPGDTRRKTND
                     ASSESIASFSKQEVMSSFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRI
                     NLEACSNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLIC
                     THFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNL
                     SMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGH
                     VPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRP
                     SNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARPSASTLLNHSFFKQIKRRASEALPE
                     LLRPVTPITNFEGSQSQDHSGIFGLVTNLEELEVDDWEF"
     exon            140..197
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            198..226
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            227..329
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            330..451
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            452..560
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            561..684
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            685..856
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            857..961
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1203
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1246
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..2166
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2137..2142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /note="hexamer: ATTAAA"
     polyA_site      2163
                     /gene="STRADA"
                     /gene_synonym="LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD
                     alpha"
                     /note="major polyA site"
ORIGIN      
        1 agtaaaactg aggaggcgga gccaagacgg tcggggctgc ttgctaactc caggaacagg
       61 tttaagtttt tgaaactgaa gtaggcctac acagtaggaa ctcatgtcat ttcttgtaag
      121 taaaccagag cgaatcaggc ggtgggtctc ggaaaagttc attgttgagg gcttaagaga
      181 tttggaacta tttggagagc agcctccggg tgacactcgg agaaaaacca atgatgcgag
      241 ctcagagtca atagcatcct tctctaaaca ggaggtcatg agtagctttc tgccagaggg
      301 agggtgttac gagctgctca ctgtgatagg caaaggattt gaggacctga tgactgtgaa
      361 tctagcaagg tacaaaccaa caggagagta cgtgactgta cggaggatta acctagaagc
      421 ttgttccaat gagatggtaa cattcttgca gggcgagctg catgtctcca aactcttcaa
      481 ccatcccaat atcgtgccat atcgagccac ttttattgca gacaatgagc tgtgggttgt
      541 cacatcattc atggcatacg gttctgcaaa agatctcatc tgtacacact tcatggatgg
      601 catgaatgag ctggcgattg cttacatcct gcagggggtg ctgaaggccc tcgactacat
      661 ccaccacatg ggatatgtac acaggagtgt caaagccagc cacatcctga tctctgtgga
      721 tgggaaggtc tacctgtctg gtttgcgcag caacctcagc atgataagcc atgggcagcg
      781 gcagcgagtg gtccacgatt ttcccaagta cagtgtcaag gttctgccgt ggctcagccc
      841 cgaggtcctc cagcagaatc tccagggtta tgatgccaag tctgacatct acagtgtggg
      901 aatcacagcc tgtgaactgg ccaacggcca tgtccccttt aaggatatgc ctgccaccca
      961 gatgctgcta gagaaactga acggcacagt gccctgcctg ttggatacca gcaccatccc
     1021 cgctgaggag ctgaccatga gcccttcgcg ctcagtggcc aactctggcc tgagtgacag
     1081 cctgaccacc agcacccccc ggccctccaa cggtgactcg ccctcccacc cctaccaccg
     1141 aaccttctcc ccccacttcc accactttgt ggagcagtgc cttcagcgca acccggatgc
     1201 caggcccagt gccagcaccc tcctgaacca ctctttcttc aagcagatca agcgacgtgc
     1261 ctcagaggct ttgcccgaat tgcttcgtcc tgtcaccccc atcaccaatt ttgagggcag
     1321 ccagtctcag gaccacagtg gaatctttgg cctggtaaca aacctggaag agctggaggt
     1381 ggacgattgg gagttctgag cctctgcaaa ctgtgcgcat tctccagcca gggatgcaga
     1441 ggccacccag aggcccttcc tgagggccgg ccacattccc gccctcctgg gcagattggg
     1501 tagaaaggac attcttccag gaaagttgac tgctgactga ttgggaaaga aaatcctgga
     1561 gagacacttc actgctccaa ggcttttgag acacaaggga atctcaacaa ccagggatca
     1621 ggagggtcca aagccgacat tcccagtcct gtgagctcag gtgacctcct ccgcagaaga
     1681 gagatgctgc tctggccctg ggagctgaat tccaagccca gggtttggct ccttaaaccc
     1741 gaggaccgcc acctcttccc agtgcttgcg accagcctca ttctatttaa ctttgctctc
     1801 agatgcctca gatgctatag gtcagtgaaa gggcaagtag taagctgcct gcctcccttc
     1861 cctcagacct ctccctcata attccagaga agggcatttc tgtcttttta agcacagact
     1921 aaggctggaa cagtccatcc ttatccctct tctggcttgg gccctgacac ctaagtcttt
     1981 cccacggttt atgtgtgtgc ctcattcctt tcccaccaag aatccatctt agcgcctcct
     2041 gccagctgcc ctggtgcttt ctccaagggc catcagtgtc ttgcctagct tgagggctta
     2101 agtccttatg ctgtgttagt ttcgttgtca gaacaaatta aaattttcag agacgctgct
     2161 ggagaa
//
