LOCUS       NM_013233               3272 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens serine/threonine kinase 39 (STK39), transcript variant
            1, mRNA.
ACCESSION   NM_013233
VERSION     NM_013233.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Li,Z., Wang,Z., Yang,S., Shen,C., Zhang,Y., Jiang,R., Zhang,Z.,
            Zhang,Y. and Hu,H.
  TITLE     CircSTK39 suppresses the proliferation and invasion of bladder
            cancer by regulating the miR-135a-5p/NR3C2-mediated
            epithelial-mesenchymal transition signaling pathway
  JOURNAL   Cell Biol Toxicol 39 (4), 1815-1834 (2023)
   PUBMED   36538242
  REMARK    GeneRIF: CircSTK39 suppresses the proliferation and invasion of
            bladder cancer by regulating the miR-135a-5p/NR3C2-mediated
            epithelial-mesenchymal transition signaling pathway.
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Boyd-Shiwarski,C.R., Shiwarski,D.J., Griffiths,S.E., Beacham,R.T.,
            Norrell,L., Morrison,D.E., Wang,J., Mann,J., Tennant,W.,
            Anderson,E.N., Franks,J., Calderon,M., Connolly,K.A., Cheema,M.U.,
            Weaver,C.J., Nkashama,L.J., Weckerly,C.C., Querry,K.E.,
            Pandey,U.B., Donnelly,C.J., Sun,D., Rodan,A.R. and Subramanya,A.R.
  TITLE     WNK kinases sense molecular crowding and rescue cell volume via
            phase separation
  JOURNAL   Cell 185 (24), 4488-4506 (2022)
   PUBMED   36318922
REFERENCE   3  (bases 1 to 3272)
  AUTHORS   Elvers,K.T., Lipka-Lloyd,M., Trueman,R.C., Bax,B.D. and Mehellou,Y.
  TITLE     Structures of the Human SPAK and OSR1 Conserved C-Terminal (CCT)
            Domains
  JOURNAL   Chembiochem 23 (1), e202100441 (2022)
   PUBMED   34726826
  REMARK    GeneRIF: Structures of the Human SPAK and OSR1 Conserved C-Terminal
            (CCT) Domains.
REFERENCE   4  (bases 1 to 3272)
  AUTHORS   Chen,J., Zhou,L., Yang,J., Xie,H., Liu,L. and Li,Y.
  TITLE     Knockdown of STK39 suppressed cell proliferation, migration, and
            invasion in hepatocellular carcinoma by repressing the
            phosphorylation of mitogen-activated protein kinase p38
  JOURNAL   Bioengineered 12 (1), 6529-6537 (2021)
   PUBMED   34519635
  REMARK    GeneRIF: Knockdown of STK39 suppressed cell proliferation,
            migration, and invasion in hepatocellular carcinoma by repressing
            the phosphorylation of mitogen-activated protein kinase p38.
REFERENCE   5  (bases 1 to 3272)
  AUTHORS   Kunnas,T., Maatta,K. and Nikkari,S.T.
  TITLE     Variant rs6749447 (T > G) in the serine threonine kinase gene is
            associated with cardiovascular complications, the Tampere adult
            population cardiovascular risk study
  JOURNAL   Medicine (Baltimore) 100 (42), e27566 (2021)
   PUBMED   34678896
  REMARK    GeneRIF: Variant rs6749447 (T > G) in the serine threonine kinase
            gene is associated with cardiovascular complications, the Tampere
            adult population cardiovascular risk study.
REFERENCE   6  (bases 1 to 3272)
  AUTHORS   Moriguchi,T., Urushiyama,S., Hisamoto,N., Iemura,S., Uchida,S.,
            Natsume,T., Matsumoto,K. and Shibuya,H.
  TITLE     WNK1 regulates phosphorylation of cation-chloride-coupled
            cotransporters via the STE20-related kinases, SPAK and OSR1
  JOURNAL   J Biol Chem 280 (52), 42685-42693 (2005)
   PUBMED   16263722
  REMARK    GeneRIF: WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling
            pathway to cation-chloride-coupled cotransporters
REFERENCE   7  (bases 1 to 3272)
  AUTHORS   Piechotta,K., Garbarini,N., England,R. and Delpire,E.
  TITLE     Characterization of the interaction of the stress kinase SPAK with
            the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a
            scaffolding role of the kinase
  JOURNAL   J Biol Chem 278 (52), 52848-52856 (2003)
   PUBMED   14563843
REFERENCE   8  (bases 1 to 3272)
  AUTHORS   Dowd,B.F. and Forbush,B.
  TITLE     PASK (proline-alanine-rich STE20-related kinase), a regulatory
            kinase of the Na-K-Cl cotransporter (NKCC1)
  JOURNAL   J Biol Chem 278 (30), 27347-27353 (2003)
   PUBMED   12740379
  REMARK    GeneRIF: roles in phosphorylation and activation of the Na-K-Cl
            cotransporter (NKCC1)
REFERENCE   9  (bases 1 to 3272)
  AUTHORS   Qi,H., Labrie,Y., Grenier,J., Fournier,A., Fillion,C. and Labrie,C.
  TITLE     Androgens induce expression of SPAK, a STE20/SPS1-related kinase,
            in LNCaP human prostate cancer cells
  JOURNAL   Mol Cell Endocrinol 182 (2), 181-192 (2001)
   PUBMED   11514053
REFERENCE   10 (bases 1 to 3272)
  AUTHORS   Johnston,A.M., Naselli,G., Gonez,L.J., Martin,R.M., Harrison,L.C.
            and DeAizpurua,H.J.
  TITLE     SPAK, a STE20/SPS1-related kinase that activates the p38 pathway
  JOURNAL   Oncogene 19 (37), 4290-4297 (2000)
   PUBMED   10980603
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF099989.1, AC016723.11 and
            BF516344.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_013233.2.
            
            Summary: This gene encodes a serine/threonine kinase that is
            thought to function in the cellular stress response pathway. The
            kinase is activated in response to hypotonic stress, leading to
            phosphorylation of several cation-chloride-coupled cotransporters.
            The catalytically active kinase specifically activates the p38 MAP
            kinase pathway, and its interaction with p38 decreases upon
            cellular stress, suggesting that this kinase may serve as an
            intermediate in the response to cellular stress. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF099989.1, SRR1803616.266955.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355999.5/ ENSP00000348278.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AF099989.1         2-52
            52-309              AF099989.1         65-322
            310-2228            AF099989.1         329-2247
            2229-3119           AC016723.11        170127-171017       c
            3120-3272           BF516344.1         17-169              c
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..3272
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /note="serine/threonine kinase 39"
                     /db_xref="GeneID:27347"
                     /db_xref="HGNC:HGNC:17717"
                     /db_xref="MIM:607648"
     exon            1..368
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1798
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine threonine kinase 39 (STE20/SPS1 homolog, yeast);
                     Ste20-like protein kinase; small intestine SPAK-like
                     kinase; proline-alanine-rich STE20-related kinase;
                     STE20/SPS1-related proline-alanine-rich protein kinase;
                     ste-20-related kinase; serine/threonine-protein kinase 39"
                     /codon_start=1
                     /product="STE20/SPS1-related proline-alanine-rich protein
                     kinase isoform 1"
                     /protein_id="NP_037365.2"
                     /db_xref="CCDS:CCDS42770.1"
                     /db_xref="GeneID:27347"
                     /db_xref="HGNC:HGNC:17717"
                     /db_xref="MIM:607648"
                     /translation="MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPA
                     PAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTS
                     MDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHK
                     NGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLA
                     TGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKY
                     PPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKC
                     KFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEK
                     SEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSC
                     AVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIV
                     DDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS"
     exon            369..481
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            482..590
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            591..732
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            733..788
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            789..898
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1000
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1134
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1183
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1249
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1372
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1402
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1465
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1536
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1589
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1658
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1723
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..3272
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3250..3255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /note="hexamer: AATAAA"
     polyA_site      3272
                     /gene="STK39"
                     /gene_synonym="DCHT; PASK; SPAK"
                     /note="major polyA site"
ORIGIN      
        1 gctccctccc ctcctagctg gcttcggcgg ggacggcggc ggcggcggcg ggcggggagg
       61 gcgtgcgccg gccgagaggt gtcggcggcg aggcaaagga agtttcaagt ggaaggtcgt
      121 ccgtcggccg gcgcgtcctc ctgctctcct ccgcagcatc atggcggagc cgagcggctc
      181 gcccgtgcac gtccagcttc cccagcaggc ggccccggtg acagcggcgg cggcggcggc
      241 cccggcggcc gcgacagcag cgccggcccc ggcagctccc gcggccccgg ccccggcccc
      301 ggccccggcg gcacaggctg tcggctggcc catctgcagg gacgcgtacg agctgcagga
      361 ggttatcggc agtggagcta ctgctgtggt tcaggcagcc ctatgcaaac ccaggcaaga
      421 acgtgtagca ataaaacgga tcaacttgga aaaatgccag accagtatgg atgaactatt
      481 aaaagaaatt caagccatga gtcagtgcag ccatcccaac gtagtgacct attacacctc
      541 ttttgtggtc aaagatgaac tttggctggt catgaaatta ctaagtggag gttcaatgtt
      601 ggatatcata aaatacattg tcaaccgagg agaacacaag aatggagttc tggaagaggc
      661 aataatagca acaattctta aagaggtttt ggaaggctta gactatctac acagaaacgg
      721 tcagattcac agggatttga aagctggtaa tattcttctg ggtgaggatg gttcagtaca
      781 aatagcagat tttggggtaa gtgcgttcct agcaacaggg ggtgatgtta cccgaaataa
      841 agtaagaaaa acattcgttg gcaccccatg ttggatggct cctgaagtca tggaacaggt
      901 gagaggctat gacttcaagg ctgacatgtg gagttttgga ataactgcca ttgaattagc
      961 aacaggagca gcgccttatc acaaatatcc tcccatgaaa gtgttaatgt tgactttgca
     1021 aaatgatcca cccactttgg aaacaggggt agaggataaa gaaatgatga aaaagtacgg
     1081 caagtccttt agaaaattac tttcactgtg tcttcagaaa gatccttcca aaaggcccac
     1141 agcagcagaa cttttaaaat gcaaattctt ccagaaagcc aagaacagag agtacctgat
     1201 tgagaagctg cttacaagaa caccagacat agcccaaaga gccaaaaagg taagaagagt
     1261 tcctgggtca agtggtcacc ttcataaaac cgaagacggg gactgggagt ggagtgacga
     1321 cgagatggat gagaagagcg aagaagggaa agcagctttt tctcaggaaa agtcacgaag
     1381 agtaaaagaa gaaaatccag agattgcagt gagtgccagc accatccccg aacaaataca
     1441 gtccctctct gtgcacgact ctcagggccc acccaatgct aatgaagact acagagaagc
     1501 ttcttcttgt gccgtgaacc tcgttttgag attaagaaac tccagaaagg aacttaatga
     1561 catacgattt gagtttactc caggaagaga tacagcagat ggtgtatctc aggagctctt
     1621 ctctgctggc ttggtggatg gtcacgatgt agttatagtg gctgctaatt tacagaagat
     1681 tgtagatgat cccaaagctt taaaaacatt gacatttaag ttggcttctg gctgtgatgg
     1741 gtcggagatt cctgatgaag tgaagctgat tgggtttgct cagttgagtg tcagctgatg
     1801 tatgtccctt gatgtcaccc tgatctgtca tgccccaccg ccacccctac tcccttcaac
     1861 cctccctctt tctgcccatt tcctcccacc ccctcactcc catttcctag caaaatcaga
     1921 agattgtgaa gaggccggct tcaacaaaat gggataaaaa aataattttt taaaacttac
     1981 aacactccga gttctgcttt attctctagc aatccacagt acaagaacaa gcaaatgcca
     2041 cagctgcacg actgttgctc atttttccaa aagctattta atattcttag caatcaattt
     2101 ggatatccct taagtgaaaa gaatctgaaa tacactcagg tggtcttatt tattggcaac
     2161 aaaaggaatt ttctatccag aagcctattt ctcctttcat tgttgttatt tctgttataa
     2221 tactttaatt gtacatctga caatactgcc tcttttatgt tgtatttaga aattaatata
     2281 cttataaaat taagatttat tagccaaact tgaattctag ttttaaaact gactgtgaat
     2341 tttatttttc atatatttat gcattacaca ccttagctat aagaaaaaaa gggttttgat
     2401 tatatgcttc ttgcagttaa tctcgttatt taaacaaaaa gttttgggtc tgtctttgga
     2461 gtatttgtaa cttctaaatt ttgaaatgac tgaattagga atttggatgc ttattctttt
     2521 agtctgtttg cctaaaaacc aatttacaat ctgactgtct cttgggagag ggaggtgcct
     2581 tgcaaacttt cacattaaga atgtgcctga ggctgcttta ctctggaata gtctcagatc
     2641 taaaatttcc tctatataag gtggcatatg ttaagttttg cttcattgga ccgtttagaa
     2701 tgctatgtaa aatgttgcca ttctgttaga ttgctaacta tatacccatc tctgatttgg
     2761 ctctccttaa gtgataggat ttgttattct aaaggtgata aacttgaaaa tatcagaatc
     2821 tgagttttac ttgaaatttt gcagaatacc caggtggagt gaaaattgga agggttttgt
     2881 gcaatgacta aaaggtaaaa cgctgttaag gttcaagaat caatactttc aacccaagta
     2941 gccctctgct tgactgtata ttatggaact agtaaacctt aggattttga aaattggagt
     3001 ctaatctttc aaggaggtgg gctcccagga tggtaccatt gctctttcct agctaaccct
     3061 agatatggca gctctttaat gtacttcaaa aagcaaatat atattactaa ggaaaaaaag
     3121 ttatttataa ttgccttgtc ataattgtta aggtgttcta gagccatttg catacaattt
     3181 aatgtaattt cattccattc tattgtttac acaacgatta ctcgaagatg actgcaaagg
     3241 taaaaggaaa ataaaagtgt attgcacaat ga
//
