LOCUS       NM_006281               2833 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens serine/threonine kinase 3 (STK3), transcript variant
            1, mRNA.
ACCESSION   NM_006281
VERSION     NM_006281.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Rodrigues,A.M., Paula Zen Petisco Fiore,A., Guardia,G.D.A.,
            Tomasin,R., Azevedo Reis Teixeira,A., Giordano,R.J., Schechtman,D.,
            Pagano,M., Galante,P.A.F. and Bruni-Cardoso,A.
  TITLE     Identification of a novel alternative splicing isoform of the Hippo
            kinase STK3/MST2 with impaired kinase and cell growth suppressing
            activities
  JOURNAL   Oncogene 43 (39), 2938-2950 (2024)
   PUBMED   39174858
  REMARK    GeneRIF: Identification of a novel alternative splicing isoform of
            the Hippo kinase STK3/MST2 with impaired kinase and cell growth
            suppressing activities.
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Zhao,Z., Chu,Y., Feng,A., Zhang,S., Wu,H., Li,Z., Sun,M., Zhang,L.,
            Chen,T. and Xu,M.
  TITLE     STK3 kinase activation inhibits tumor proliferation through
            FOXO1-TP53INP1/P21 pathway in esophageal squamous cell carcinoma
  JOURNAL   Cell Oncol (Dordr) 47 (4), 1295-1314 (2024)
   PUBMED   38436783
  REMARK    GeneRIF: STK3 kinase activation inhibits tumor proliferation
            through FOXO1-TP53INP1/P21 pathway in esophageal squamous cell
            carcinoma.
REFERENCE   3  (bases 1 to 2833)
  AUTHORS   Jeong,D.J., Um,J.H., Kim,Y.Y., Shin,D.J., Im,S., Lee,K.M.,
            Lee,Y.H., Lim,D.S., Kim,D. and Yun,J.
  TITLE     The Mst1/2-BNIP3 axis is required for mitophagy induction and
            neuronal viability under mitochondrial stress
  JOURNAL   Exp Mol Med 56 (3), 674-685 (2024)
   PUBMED   38443598
  REMARK    GeneRIF: The Mst1/2-BNIP3 axis is required for mitophagy induction
            and neuronal viability under mitochondrial stress.
REFERENCE   4  (bases 1 to 2833)
  AUTHORS   Ma,L., Gao,F., Dong,W., Song,Q. and Jia,Y.
  TITLE     Association Study between SNPs in MST1 and MST2 and H. pylori
            Infection as well as Noncardia Gastric Carcinogenesis
  JOURNAL   Dig Dis 42 (3), 230-239 (2024)
   PUBMED   38295774
  REMARK    GeneRIF: Association Study between SNPs in MST1 and MST2 and H.
            pylori Infection as well as Noncardia Gastric Carcinogenesis.
REFERENCE   5  (bases 1 to 2833)
  AUTHORS   Sun,Y., Jin,X., Meng,J., Guo,F., Chen,T., Zhao,X., Wu,H. and Ren,D.
  TITLE     MST2 methylation by PRMT5 inhibits Hippo signaling and promotes
            pancreatic cancer progression
  JOURNAL   EMBO J 42 (23), e114558 (2023)
   PUBMED   37905571
  REMARK    GeneRIF: MST2 methylation by PRMT5 inhibits Hippo signaling and
            promotes pancreatic cancer progression.
REFERENCE   6  (bases 1 to 2833)
  AUTHORS   Bren,A., Welch,M., Blat,Y. and Eisenbach,M.
  TITLE     Signal termination in bacterial chemotaxis: CheZ mediates
            dephosphorylation of free rather than switch-bound CheY
  JOURNAL   Proc Natl Acad Sci U S A 93 (19), 10090-10093 (1996)
   PUBMED   8816756
REFERENCE   7  (bases 1 to 2833)
  AUTHORS   Creasy,C.L. and Chernoff,J.
  TITLE     Cloning and characterization of a member of the MST subfamily of
            Ste20-like kinases
  JOURNAL   Gene 167 (1-2), 303-306 (1995)
   PUBMED   8566796
REFERENCE   8  (bases 1 to 2833)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   9  (bases 1 to 2833)
  AUTHORS   Creasy,C.L. and Chernoff,J.
  TITLE     Cloning and characterization of a human protein kinase with
            homology to Ste20
  JOURNAL   J Biol Chem 270 (37), 21695-21700 (1995)
   PUBMED   7665586
REFERENCE   10 (bases 1 to 2833)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP003551.2, U26424.1 and BC010640.2.
            
            On Nov 23, 2018 this sequence version replaced NM_006281.3.
            
            Summary: This gene encodes a serine/threonine protein kinase
            activated by proapoptotic molecules indicating the encoded protein
            functions as a growth suppressor. Cleavage of the protein product
            by caspase removes the inhibitory C-terminal portion. The
            N-terminal portion is transported to the nucleus where it
            homodimerizes to form the active kinase which promotes the
            condensation of chromatin during apoptosis. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010640.2, SRR1660809.119934.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000419617.7/ ENSP00000390500.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AP003551.2         155726-155732       c
            8-18                U26424.1           1-11
            19-2833             BC010640.2         1-2815
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q22.2"
     gene            1..2833
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /note="serine/threonine kinase 3"
                     /db_xref="GeneID:6788"
                     /db_xref="HGNC:HGNC:11406"
                     /db_xref="MIM:605030"
     exon            1..174
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..1624
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine kinase 3 (STE20 homolog, yeast);
                     serine/threonine kinase 3 (Ste20, yeast homolog);
                     serine/threonine-protein kinase 3; MST-2; STE20-like
                     kinase MST2; hippo homolog; mammalian STE20-like protein
                     kinase 2; serine/threonine-protein kinase Krs-1;
                     KB-1458E12.1; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="serine/threonine-protein kinase 3 isoform 1"
                     /protein_id="NP_006272.2"
                     /db_xref="CCDS:CCDS47900.1"
                     /db_xref="GeneID:6788"
                     /db_xref="HGNC:HGNC:11406"
                     /db_xref="MIM:605030"
                     /translation="MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKA
                     IHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYC
                     GAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHA
                     KLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGK
                     PPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHP
                     FIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESV
                     GTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPS
                     FMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQM
                     RLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF"
     exon            175..255
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            256..384
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            385..499
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            500..664
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            665..832
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            833..970
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1096
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1289
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1465
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..2833
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2808..2813
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /note="hexamer: AATAAA"
     polyA_site      2833
                     /gene="STK3"
                     /gene_synonym="KRS1; MST2"
                     /note="major polyA site"
ORIGIN      
        1 agatccgccg cggagttacg ggaaagttgg tccgagttcc cagagtttcc ctctgtggtg
       61 ccctaggctc ggccggccgg tgccccggct cctttcctcc tttcggcctt cgccgtccac
      121 caggtccctc tctctgtccc cggccgccat ggagcagccg ccggcgccta agagtaaact
      181 aaaaaagctg agtgaagaca gtttgactaa gcagcctgaa gaagtttttg atgtattaga
      241 gaagcttgga gaagggtctt atggaagtgt atttaaagca atacacaagg aatccggtca
      301 agttgtcgca attaaacaag tacctgttga atcagatctt caggaaataa tcaaagaaat
      361 ttccataatg cagcaatgtg acagcccata tgttgtaaag tactatggca gttattttaa
      421 gaatacagac ctctggattg ttatggagta ctgtggcgct ggctctgtct cagacataat
      481 tagattacga aacaagacat taatagaaga tgaaattgca accattctta aatctacatt
      541 gaaaggacta gaatatttgc actttatgag aaaaatacac agagatataa aagctggaaa
      601 tattctcctc aatacagaag gacatgcaaa attggcagat tttggagtgg ctggtcagtt
      661 aacagataca atggcaaaac gcaatactgt aataggaact ccattttgga tggctcctga
      721 ggtgattcaa gaaataggct ataactgtgt ggccgacatc tggtcccttg gcattacttc
      781 tatagaaatg gctgaaggaa aacctcctta tgctgatata catccaatga gggctatttt
      841 tatgattccc acaaatccac caccaacatt cagaaagcca gaactttggt ccgatgattt
      901 caccgatttt gttaaaaagt gtttggtgaa gaatcctgag cagagagcta ctgcaacaca
      961 acttttacag catcctttta tcaagaatgc caaacctgta tcaatattaa gagacctgat
     1021 cacagaagct atggagatca aagctaaaag acatgaggaa cagcaacgag aattggaaga
     1081 ggaagaagaa aattcggatg aagatgagct ggattcccac accatggtga agactagtgt
     1141 ggagagtgtg ggcaccatgc gggccacaag cacgatgagt gaaggggccc agaccatgat
     1201 tgaacataat agcacgatgt tggaatccga cttggggacc atggtgataa acagtgagga
     1261 tgaggaagaa gaagatggaa ctatgaaaag aaatgcaacc tcaccacaag tacaaagacc
     1321 atctttcatg gactactttg ataagcaaga cttcaagaat aagagtcacg aaaactgtaa
     1381 tcagaacatg catgaaccct tccctatgtc caaaaacgtt tttcctgata actggaaagt
     1441 tcctcaagat ggagactttg actttttgaa aaatctaagt ttagaagaac tacagatgcg
     1501 gttaaaagca ctggacccca tgatggaacg ggagatagaa gaacttcgtc agagatacac
     1561 tgcgaaaaga cagcccattc tggatgcgat ggatgcaaag aaaagaaggc agcaaaactt
     1621 ttgagtctaa tttcctctct gtttttaact attctggaga ccaagaaacc actaggaatt
     1681 gaaggaatat ttggatattt ttaatcctaa gattttgccc tacaattagg cagaggtcaa
     1741 aaagtgacaa tggtacatgc ccaggtaaat tcccaaaagg cagaattgac agttgtatct
     1801 gctgtgcatt cactctaaga tgaggagaac aaaagaagtg tattctcttg ttctgtcagc
     1861 tgcataccag taataaaact gttatgaaat ggattttcaa ggtctctaaa ccttgaaaat
     1921 ccaaagctat tgttgcattg tacagcactg aagggcttta tgttacaata ttctttattc
     1981 ctatctagta tactaggcta tttattgtat ccccttaggt aaacttattt atttatgcta
     2041 ttttgctttg tttcattttt taaggacaag atcaggatag ctttggtgaa ggtagggtca
     2101 tattaatatg atgataatgt gcaaccaatt tatactttct gcagggagct atggggtaca
     2161 ttccttgatt tccaggatag tttttcaaat aggaaagcaa taatggcagt agttctcaaa
     2221 tgggctaggc cttttttata ttgaagcaat aattccattt ttaccctttg aaattttgtt
     2281 tttttgattt ttgatgtttg gtacaaatag aactatatat atttaggtaa aatagatcta
     2341 tcgtgtttaa aaccaaagaa atcaatggaa cccttgcaca aaaaagtgtg ataaatattt
     2401 ttaaataaaa acttaataca aatgtaattt gttaatattg tttcatgttt tatgtgtaga
     2461 tctaatagct gaactgattc aaactgtaat aagctcatca atttcatttc tatgaaaatg
     2521 tgctctgttg tcacaggatg tttctgttga ttttattcat ttcctgggaa ttggtaaaca
     2581 tcatgttcct gatgataacc cagtagcaaa aacatttgta ctgagtggta caagccttgg
     2641 ggactgaaaa aaaaaagatt aaaaccatta aaaagaaact catttttacg ctgaatgaac
     2701 atttatatga ttgcattggg accagtcatt tcctaagcta catatggcca tcttgacagt
     2761 gttttttctt ttgtgtgttt aattattatg tgtaaatcat aaagacaaat aaatttcact
     2821 gtgccaccca gca
//
