LOCUS       NP_001027467             431 aa            linear   PRI 30-APR-2025
DEFINITION  serine/threonine-protein kinase 24 isoform b precursor [Homo
            sapiens].
ACCESSION   NP_001027467
VERSION     NP_001027467.2
DBSOURCE    REFSEQ: accession NM_001032296.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 431)
  AUTHORS   Wang,N., Jiang,Y., Li,M., Wang,H., Pan,J., Tang,Y., Xie,S., Xu,Y.,
            Li,X., Zhou,X., Xu,P., Lin,W. and Wang,X.
  TITLE     Protein Kinase STK24 Promotes Tumor Immune Evasion via the
            AKT-PD-L1 Axis
  JOURNAL   Adv Sci (Weinh) 11 (12), e2304342 (2024)
   PUBMED   38229183
  REMARK    GeneRIF: Protein Kinase STK24 Promotes Tumor Immune Evasion via the
            AKT-PD-L1 Axis.
REFERENCE   2  (residues 1 to 431)
  AUTHORS   Caputo,M., Xia,Y., Anand,S.K., Cansby,E., Andersson,E.,
            Marschall,H.U., Konigsrainer,A., Peter,A. and Mahlapuu,M.
  TITLE     STE20-type kinases MST3 and MST4 promote the progression of
            hepatocellular carcinoma: Evidence from human cell culture and
            expression profiling of liver biopsies
  JOURNAL   FASEB J 37 (8), e23105 (2023)
   PUBMED   37490000
  REMARK    GeneRIF: STE20-type kinases MST3 and MST4 promote the progression
            of hepatocellular carcinoma: Evidence from human cell culture and
            expression profiling of liver biopsies.
REFERENCE   3  (residues 1 to 431)
  AUTHORS   Lai,S., Wang,D., Sun,W. and Cao,X.
  TITLE     Serine/threonine-protein kinase STK24 induces tumorigenesis by
            regulating the STAT3/VEGFA signaling pathway
  JOURNAL   J Biol Chem 299 (3), 102961 (2023)
   PUBMED   36720310
  REMARK    GeneRIF: Serine/threonine-protein kinase STK24 induces
            tumorigenesis by regulating the STAT3/VEGFA signaling pathway.
REFERENCE   4  (residues 1 to 431)
  AUTHORS   Sugden,P.H., McGuffin,L.J. and Clerk,A.
  TITLE     SOcK, MiSTs, MASK and STicKs: the GCKIII (germinal centre kinase
            III) kinases and their heterologous protein-protein interactions
  JOURNAL   Biochem J 454 (1), 13-30 (2013)
   PUBMED   23889253
  REMARK    GeneRIF: This review notes that in the SOK1 and MST4 germinal
            center kinase III family of proteins, exon 1 encodes a 5'
            untranslated region, but this is not the case for MST3.
            Review article
REFERENCE   5  (residues 1 to 431)
  AUTHORS   Stegert,M.R., Hergovich,A., Tamaskovic,R., Bichsel,S.J. and
            Hemmings,B.A.
  TITLE     Regulation of NDR protein kinase by hydrophobic motif
            phosphorylation mediated by the mammalian Ste20-like kinase MST3
  JOURNAL   Mol Cell Biol 25 (24), 11019-11029 (2005)
   PUBMED   16314523
  REMARK    GeneRIF: Activation of NDR2 is a multistep process involving
            phosphorylation of the hydrophobic motif site Thr444/2 by MST3.
REFERENCE   6  (residues 1 to 431)
  AUTHORS   Lu,T.J., Huang,C.Y., Yuan,C.J., Lee,Y.C., Leu,T.H., Chang,W.C.,
            Lu,T.L., Jeng,W.Y. and Lai,M.D.
  TITLE     Zinc ion acts as a cofactor for serine/threonine kinase MST3 and
            has a distinct role in autophosphorylation of MST3
  JOURNAL   J Inorg Biochem 99 (6), 1306-1313 (2005)
   PUBMED   15917084
REFERENCE   7  (residues 1 to 431)
  AUTHORS   Lee,W.S., Hsu,C.Y., Wang,P.L., Huang,C.Y., Chang,C.H. and Yuan,C.J.
  TITLE     Identification and characterization of the nuclear import and
            export signals of the mammalian Ste20-like protein kinase 3
  JOURNAL   FEBS Lett 572 (1-3), 41-45 (2004)
   PUBMED   15304321
  REMARK    GeneRIF: Mst3 contains signals which may cooperate to control the
            subcellular distribution of Mst3.
REFERENCE   8  (residues 1 to 431)
  AUTHORS   Huang,C.Y., Wu,Y.M., Hsu,C.Y., Lee,W.S., Lai,M.D., Lu,T.J.,
            Huang,C.L., Leu,T.H., Shih,H.M., Fang,H.I., Robinson,D.R.,
            Kung,H.J. and Yuan,C.J.
  TITLE     Caspase activation of mammalian sterile 20-like kinase 3 (Mst3).
            Nuclear translocation and induction of apoptosis
  JOURNAL   J Biol Chem 277 (37), 34367-34374 (2002)
   PUBMED   12107159
  REMARK    GeneRIF: Proteolytic activation of Mst3 by caspases.
REFERENCE   9  (residues 1 to 431)
  AUTHORS   Zhou,T.H., Ling,K., Guo,J., Zhou,H., Wu,Y.L., Jing,Q., Ma,L. and
            Pei,G.
  TITLE     Identification of a human brain-specific isoform of mammalian
            STE20-like kinase 3 that is regulated by cAMP-dependent protein
            kinase
  JOURNAL   J Biol Chem 275 (4), 2513-2519 (2000)
   PUBMED   10644707
REFERENCE   10 (residues 1 to 431)
  AUTHORS   Schinkmann,K. and Blenis,J.
  TITLE     Cloning and characterization of a human STE20-like protein kinase
            with unusual cofactor requirements
  JOURNAL   J Biol Chem 272 (45), 28695-28703 (1997)
   PUBMED   9353338
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356423.15 and AL137249.29.
            
            On Jul 14, 2006 this sequence version replaced NP_001027467.1.
            
            Summary: This gene encodes a serine/threonine protein kinase that
            functions upstream of mitogen-activated protein kinase (MAPK)
            signaling. The encoded protein is cleaved into two chains by
            caspases; the N-terminal fragment (MST3/N) translocates to the
            nucleus and promotes programmed cells death. There is a pseudogene
            for this gene on chromosome X. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at an
            alternate start codon, compared to variant 1. The encoded isoform
            (b) has a distinct N-terminus and is shorter than isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU668350.1, BC065378.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000539966.6/ ENSP00000442539.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q32.2"
     Protein         1..431
                     /product="serine/threonine-protein kinase 24 isoform b
                     precursor"
                     /EC_number="2.7.11.1"
                     /note="STE20-like kinase 3; sterile 20-like kinase 3;
                     serine/threonine kinase 24 (STE20 homolog, yeast);
                     serine/threonine-protein kinase 24; STE20-like kinase
                     MST3; mammalian STE20-like protein kinase 3; epididymis
                     secretory protein Li 95"
                     /calculated_mol_wt=47782
     mat_peptide     1..313
                     /product="mammalian STE20-like protein kinase 3 N-terminal
                     chain; MST3/N"
                     /calculated_mol_wt=35246
     Region          22..295
                     /region_name="STKc_MST3_like"
                     /note="Catalytic domain of Mammalian Ste20-like protein
                     kinase 3-like Serine/Threonine Kinases; cd06609"
                     /db_xref="CDD:270786"
     Site            order(30..34,38,51,53,83,99..102,105..106,109,144,
                     146..149,151,161..162,165,179..182,184,211,220)
                     /site_type="active"
                     /db_xref="CDD:270786"
     Site            order(30..34,38,51,53,70,83,99..102,105..106,109,148..149,
                     151,162)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270786"
     Site            order(33..34,144,146..148,165,179..182,184,211,220)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270786"
     Site            order(161..172,174..184)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270786"
     mat_peptide     314..431
                     /product="mammalian STE20-like protein kinase 3 C-terminal
                     chain; MST3/C"
                     /calculated_mol_wt=12685
     CDS             1..431
                     /gene="STK24"
                     /gene_synonym="HEL-S-95; MST3; MST3B; STE20; STK3"
                     /coded_by="NM_001032296.4:322..1617"
                     /note="isoform b precursor is encoded by transcript
                     variant 2"
                     /db_xref="CCDS:CCDS32001.1"
                     /db_xref="GeneID:8428"
                     /db_xref="HGNC:HGNC:11403"
                     /db_xref="MIM:604984"
ORIGIN      
        1 mahspvqsgl pgmqnlkadp eelftkleki gkgsfgevfk gidnrtqkvv aikiidleea
       61 edeiediqqe itvlsqcdsp yvtkyygsyl kdtklwiime ylgggsaldl lepgpldetq
      121 iatilreilk gldylhsekk ihrdikaanv llsehgevkl adfgvagqlt dtqikrntfv
      181 gtpfwmapev ikqsaydska diwslgitai elargepphs elhpmkvlfl ipknnpptle
      241 gnyskplkef veaclnkeps frptakellk hkfilrnakk tsyltelidr ykrwkaeqsh
      301 ddsssedsda etdgqasggs dsgdwiftir ekdpknleng alqpsdldrn kmkdipkrpf
      361 sqclstiisp lfaelkeksq acggnlgsie elrgaiylae eacpgisdtm vaqlvqrlqr
      421 yslsgggtss h
//
