LOCUS       NM_020860               5004 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens stromal interaction molecule 2 (STIM2), transcript
            variant 2, mRNA.
ACCESSION   NM_020860
VERSION     NM_020860.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Djordjevic,S., Itzykson,R., Hague,F., Lebon,D., Legrand,J.,
            Ouled-Haddou,H., Jedraszak,G., Harbonnier,J., Collet,L.,
            Paubelle,E., Marolleau,J.P., Garcon,L. and Boyer,T.
  TITLE     STIM2 is involved in the regulation of apoptosis and the cell cycle
            in normal and malignant monocytic cells
  JOURNAL   Mol Oncol 18 (6), 1571-1592 (2024)
   PUBMED   38234211
  REMARK    GeneRIF: STIM2 is involved in the regulation of apoptosis and the
            cell cycle in normal and malignant monocytic cells.
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5004)
  AUTHORS   Cheng,H., Chen,L., Fang,Z., Wan,Q., Du,Z., Ma,N., Guo,G. and Lu,W.
  TITLE     STIM2 promotes the invasion and metastasis of breast cancer cells
            through the NFAT1/TGF-beta1 pathway
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 67 (6), 55-61 (2022)
   PUBMED   35818214
  REMARK    GeneRIF: STIM2 promotes the invasion and metastasis of breast
            cancer cells through the NFAT1/TGF-beta1 pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5004)
  AUTHORS   Rana,A., Yen,M., Sadaghiani,A.M., Malmersjo,S., Park,C.Y.,
            Dolmetsch,R.E. and Lewis,R.S.
  TITLE     Alternative splicing converts STIM2 from an activator to an
            inhibitor of store-operated calcium channels
  JOURNAL   J Cell Biol 209 (5), 653-669 (2015)
   PUBMED   26033257
  REMARK    GeneRIF: STIM2beta does not by itself strongly bind Orai1, it is
            recruited to Orai1 channels by forming heterodimers with other STIM
            isoforms.
REFERENCE   5  (bases 1 to 5004)
  AUTHORS   Miederer,A.M., Alansary,D., Schwar,G., Lee,P.H., Jung,M., Helms,V.
            and Niemeyer,B.A.
  TITLE     A STIM2 splice variant negatively regulates store-operated calcium
            entry
  JOURNAL   Nat Commun 6, 6899 (2015)
   PUBMED   25896806
  REMARK    GeneRIF: These results imply that the splice variant STIM2.1 is an
            additional player tuning Orai1 activation in vivo.
            GeneRIF: STIM2.1 cannot activate Orai1 due to splicing in residues
            within the channel-activating domain (CAD). STIM2.1 confers a
            calcium dependent dominant-negative function on both STIM1 and
            STIM2 (STIM2.2). Affinity of the STIM2.1 calmodulin binding site
            within the CAD domain is increased compared to STIM2.2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5004)
  AUTHORS   Soboloff,J., Spassova,M.A., Hewavitharana,T., He,L.P., Xu,W.,
            Johnstone,L.S., Dziadek,M.A. and Gill,D.L.
  TITLE     STIM2 is an inhibitor of STIM1-mediated store-operated Ca2+ Entry
  JOURNAL   Curr Biol 16 (14), 1465-1470 (2006)
   PUBMED   16860747
  REMARK    GeneRIF: STIM2 is an inhibitor of store-operated channel (SOC)
            activation and plays a coordinated role with STIM1 in controlling
            SOC-mediated Ca(2+) entry signals.
REFERENCE   7  (bases 1 to 5004)
  AUTHORS   Liou,J., Kim,M.L., Heo,W.D., Jones,J.T., Myers,J.W., Ferrell,J.E.
            Jr. and Meyer,T.
  TITLE     STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered
            Ca2+ influx
  JOURNAL   Curr Biol 15 (13), 1235-1241 (2005)
   PUBMED   16005298
  REMARK    GeneRIF: STIM proteins function as Ca(2+) store sensors in the
            signaling pathway connecting Ca(2+) store depletion to Ca(2+)
            influx.
REFERENCE   8  (bases 1 to 5004)
  AUTHORS   Williams,R.T., Manji,S.S., Parker,N.J., Hancock,M.S., Van
            Stekelenburg,L., Eid,J.P., Senior,P.V., Kazenwadel,J.S.,
            Shandala,T., Saint,R., Smith,P.J. and Dziadek,M.A.
  TITLE     Identification and characterization of the STIM (stromal
            interaction molecule) gene family: coding for a novel class of
            transmembrane proteins
  JOURNAL   Biochem J 357 (Pt 3), 673-685 (2001)
   PUBMED   11463338
  REMARK    GeneRIF: Translation initiation from a non-AUG (UUG) start site.
REFERENCE   9  (bases 1 to 5004)
  AUTHORS   Irvin,A.D., Young,E.R., Luther,P.D. and Collins,A.P.
  TITLE     Interspecific fusion of bovine and other cells with parainfluenza
            viruses (Sendai and Pi-3)
  JOURNAL   J Comp Pathol 87 (3), 393-404 (1977)
   PUBMED   198439
  REMARK    GeneRIF: STIM2 is involved in spatial learning and plays an
            important role in ischemic-induced neuronal cell death during brain
            hypoxia. The absence of STIM2 reduces life expectancy in mice by
            sudden death.
REFERENCE   10 (bases 1 to 5004)
  AUTHORS   Mchajlow,W.
  TITLE     [Prospects for the development of parasitologic science]
  JOURNAL   Wiad Parazytol 21 (3), 355-365 (1975)
   PUBMED   1146333
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC106047.5 and AC006928.15.
            
            On Jun 2, 2019 this sequence version replaced NM_020860.3.
            
            Summary: This gene is a member of the stromal interaction molecule
            (STIM) family and likely arose, along with related family member
            STIM1, from a common ancestral gene. The encoded protein functions
            to regulate calcium concentrations in the cytosol and endoplasmic
            reticulum, and is involved in the activation of plasma membrane
            Orai Ca(2+) entry channels. This gene initiates translation from a
            non-AUG (UUG) start site. A signal peptide is cleaved from the
            resulting protein. Multiple transcript variants result from
            alternative splicing. [provided by RefSeq, Dec 2009].
            
            Transcript Variant: This variant (2, also known as STIM2.2 or
            STIM2alpha) lacks an alternate in-frame exon in the central coding
            region, compared to variant 1, resulting in an isoform (2) that is
            shorter than isoform 1. This isoform functions as a positive
            regulator of store-operated calcium entry (SOCE).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB040915.1, SRR1803617.151765.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:11463338)
            MANE Ensembl match               :: ENST00000467087.7/
                                                ENSP00000419073.2
            non-AUG initiation codon         :: PMID: 11463338
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-529               AC106047.5         79669-80197
            530-660             AC106047.5         138332-138462
            661-775             AC006928.15        166836-166950       c
            776-887             AC006928.15        129072-129183       c
            888-1003            AC006928.15        125215-125330       c
            1004-1181           AC006928.15        122168-122345       c
            1182-1359           AC006928.15        121458-121635       c
            1360-1527           AC006928.15        116862-117029       c
            1528-1628           AC006928.15        116034-116134       c
            1629-1867           AC006928.15        115560-115798       c
            1868-2141           AC006928.15        106578-106851       c
            2142-5004           AC006928.15        99181-102043        c
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.2"
     gene            1..5004
                     /gene="STIM2"
                     /note="stromal interaction molecule 2"
                     /db_xref="GeneID:57620"
                     /db_xref="HGNC:HGNC:19205"
                     /db_xref="MIM:610841"
     exon            1..529
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..2619
                     /gene="STIM2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; non-AUG (UUG) translation initiation codon"
                     /codon_start=1
                     /product="stromal interaction molecule 2 isoform 2
                     precursor"
                     /protein_id="NP_065911.3"
                     /db_xref="CCDS:CCDS3440.2"
                     /db_xref="GeneID:57620"
                     /db_xref="HGNC:HGNC:19205"
                     /db_xref="MIM:610841"
                     /translation="MLVLGLLVAGAADGCELVPRHLRGRRATGSAATAASSPAAAAGD
                     SPALMTDPCMSLSPPCFTEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKY
                     KDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRD
                     NNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDF
                     ILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEEN
                     RNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKK
                     AEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRS
                     TVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSG
                     LPSLTSSLYSDHSWVVMPRVSIPPYPIAGGVDDLDEDTPPIVSQFPGTMAKPPGSLAR
                     SSSLCRSRRSIVPSSPQPQRAQLAPHAPHPSHPRHPHHPQHTPHSLPSPDPDILSVSS
                     CPALYRNEEEEEAIYFSAEKQWEVPDTASECDSLNSSIGRKQSPPLSLEIYQTLSPRK
                     ISRDEVSLEDSSRGDSPVTVDVSWGSPDCVGLTETKSMIFSPASKVYNGILEKSCSMN
                     QLSSGIPVPKPRHTSCSSAGNDSKPVQEAPSVARISSIPHDLCHNGEKSKKPSKIKSL
                     FKKKSK"
     exon            530..660
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            661..775
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            776..887
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1003
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1181
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1359
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1527
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1628
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1867
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..2141
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..5004
                     /gene="STIM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3567..3572
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STIM2"
                     /note="hexamer: AATAAA"
     polyA_site      3584
                     /gene="STIM2"
                     /note="major polyA site"
     regulatory      3788..3793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STIM2"
                     /note="hexamer: TATAAA"
     polyA_site      3808
                     /gene="STIM2"
                     /note="major polyA site"
     regulatory      3827..3832
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STIM2"
                     /note="hexamer: TATAAA"
     regulatory      3844..3849
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STIM2"
                     /note="hexamer: ATTAAA"
     polyA_site      3849
                     /gene="STIM2"
     polyA_site      3862
                     /gene="STIM2"
     regulatory      4186..4191
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STIM2"
                     /note="hexamer: TATAAA"
     polyA_site      4209
                     /gene="STIM2"
ORIGIN      
        1 agagcagcgg atcccggtct cgccgcagca gcagcgcggg tgtcgtgcac cgcctgaaga
       61 cgccgtacct ttctaccccc cacctttttt tttttttttt ttaaataacc ggaaccaatg
      121 aacgcagccg ggatcagagc tccggaggcc gccggtgccg atgggaccag gctggcgccc
      181 ggcgggagcc cgtgtctgag gcggcggggg cggccggagg agtcgccggc ggcggtggtg
      241 gcgcctcgcg gagccggcga gctgcaggcg gccggggcgc cgctgcgctt tcacccggct
      301 tctcctcggc gccttcatcc cgcctcgact cctggcccag cgtggggctg gctgctgcgg
      361 cggcggcgct gggctgcgtt gctggtgctc gggctgctgg tagccggagc ggcggacgga
      421 tgcgagcttg tgccccggca cctccgcggg cggcgggcga ctggctctgc cgcaactgcc
      481 gcctcctctc ccgccgcggc ggccggcgat agcccggcgc tcatgacaga tccctgcatg
      541 tcactgagtc caccatgctt tacagaagaa gacagattta gtctggaagc tcttcaaaca
      601 atacataaac aaatggatga tgacaaagat ggtggaattg aagtagagga aagtgatgaa
      661 ttcatcagag aagatatgaa atataaagat gctactaata aacacagcca tctgcacaga
      721 gaagataaac atataacgat tgaggattta tggaaacgat ggaaaacatc agaagttcat
      781 aattggaccc ttgaagacac tcttcagtgg ttgatagagt ttgttgaact accccaatat
      841 gagaagaatt ttagagacaa caatgtcaaa ggaacgacac ttcccaggat agcagtgcac
      901 gaaccttcat ttatgatctc ccagttgaaa atcagtgacc ggagtcacag acaaaaactt
      961 cagctcaagg cattggatgt ggttttgttt ggacctctaa cacgcccacc tcataactgg
     1021 atgaaagatt ttatcctcac agtttctata gtaattggtg ttggaggctg ctggtttgct
     1081 tatacgcaga ataagacatc aaaagaacat gttgcaaaaa tgatgaaaga tttagagagc
     1141 ttacaaactg cagagcaaag tctaatggac ttacaggaga ggcttgaaaa ggcacaggaa
     1201 gaaaacagaa atgttgctgt agaaaagcaa aatttagagc gcaaaatgat ggatgaaatc
     1261 aattatgcaa aggaggaggc ttgtcggctg agagagctaa gggagggagc tgaatgtgaa
     1321 ttgagtagac gtcagtatgc agaacaggaa ttggaacagg ttcgcatggc tctgaaaaag
     1381 gccgaaaaag aatttgaact gagaagcagt tggtctgttc cagatgcact tcagaaatgg
     1441 cttcagttaa cacatgaagt agaagtgcaa tactacaata ttaaaagaca aaacgctgaa
     1501 atgcagctag ctattgctaa agatgaggca gaaaaaatta aaaagaagag aagcacagtc
     1561 tttgggactc tgcacgttgc acacagctcc tccctagatg aggtagacca caaaattctg
     1621 gaagcaaaga aagctctctc tgagttgaca acttgtttac gagaacgact ttttcgctgg
     1681 caacaaattg agaagatctg tggctttcag atagcccata actcaggact ccccagcctg
     1741 acctcttccc tttattctga tcacagctgg gtggtgatgc ccagagtctc cattccaccc
     1801 tatccaattg ctggaggagt tgatgactta gatgaagaca cacccccaat agtgtcacaa
     1861 tttcccggga ccatggctaa acctcctgga tcattagcca gaagcagcag cctgtgccgt
     1921 tcacgccgca gcattgtgcc gtcctcgcct cagcctcagc gagctcagct tgctccacac
     1981 gccccccacc cgtcacaccc tcggcaccct caccacccgc aacacacacc acactccttg
     2041 ccttcccctg atccagatat cctctcagtg tcaagttgcc ctgcgcttta tcgaaatgaa
     2101 gaggaggaag aggccattta cttctctgct gaaaagcaat gggaagtgcc agacacagct
     2161 tcagaatgtg actccttaaa ttcttccatt ggaaggaaac agtctcctcc tttaagcctc
     2221 gagatatacc aaacattatc tccgcgaaag atatcaagag atgaggtgtc cctagaggat
     2281 tcctcccgag gggattcgcc tgtaactgtg gatgtgtctt ggggttctcc cgactgtgta
     2341 ggtctgacag aaactaagag tatgatcttc agtcctgcaa gcaaagtgta caatggcatt
     2401 ttggagaaat cctgtagcat gaaccagctt tccagtggca tcccggtgcc taaacctcgc
     2461 cacacatcat gttcctcagc tggcaacgac agtaaaccag ttcaggaagc cccaagtgtt
     2521 gccagaataa gcagcatccc acatgacctt tgtcataatg gagagaaaag caaaaagcca
     2581 tcaaaaatca aaagcctttt taagaagaaa tctaagtgaa ctggctgact tgatggaatc
     2641 atgttcaagt ggcatctgta aactattatc ccccaccctc cactccccac cttttttttg
     2701 gtttaatttt aggaatgtaa ctccattggg gctttccagg ccggatgcca tagtggaaca
     2761 tccagaaggg caactgtcta ctgtctgctt atttaagtga ctatatataa tcaattcatc
     2821 aagccagtta ttactgaaaa atcattgaaa tgagacagtt tacagtcatt tctgcctatt
     2881 tatttctgct ttgttctcag tgatgtatat gcaacatttt gttgaaagcc acgatggact
     2941 tacaagcttt aatggactcg taagccagca tgggcttgca aaaatttctt gtttaccaga
     3001 gcatcttctt atctttccac agagctattt acatcctgga ctatataact taaaagaagt
     3061 aaaacgtaat tgcactactg ttttccagac tggaaaaaaa aaaaatctct gcaagtgaaa
     3121 ctgtatagag tttataaaat gactatggat aggggactgt tttcactttt agatcaaaat
     3181 gggtttttaa gtagaaccta gggtttctaa ttgacttgat ttctggaaat gaaaacccgc
     3241 gcttttatta tgggaagctt cttgaactgc atttactatt gtgaagtttc aagtcccgct
     3301 gtaaagatca tgttgttttg ttttccccag ggctttcact gtgatttact gcattgcagg
     3361 ctgtatgata aaacacacat aatttaaaga gagaaggctc ttgattcctt atgcaagtgg
     3421 aagagttgaa acttgattga aggacttaaa acattcacaa ccttaagccg aggtgggggg
     3481 atatggggat tcaggcaatt gtttacacac tttgaataac tgcaaaggat ttacggtttg
     3541 tgaaaaatgt gtactgtgga aaagataata aattgaagac attattgtgt gggattgtgc
     3601 tgatttttgt tgataacaca aaaaacacta tgttttctgg agagctgtgt aagctgtctt
     3661 gttgcttagt tgcaatataa gaaatagtga tgttttggac gtaagttgtc aacaaatttc
     3721 tattttatat tgttatattt ttatgtagtt tgaaatgtaa aaatgttcta atatcaagat
     3781 taacaaatat aaatttatgg tgcatttaga ttgcgttgta ttaatttata aagtatggtg
     3841 ttcattaaat tggtgggttg tataagggta gcttttgttg ttaaaaaaac agtagagagt
     3901 taagttccat atagcaacaa agtatgttaa catctaggga gtttctgctt ataccacttt
     3961 aaaaatatgc aatcataagt gatttggtta ctgcaatgca gatggatgtt tggatgaaca
     4021 actgatacat tttagttaga atgctttttc agcattatgg ctaaaatata tgtctaaccc
     4081 agtgctattt aggtgaaaat gctaattgat aaaccagaag tttcttttga gatttgcttt
     4141 aaagaaatgc tttagtaaga atatgaacaa tttcctgatg tctcctataa attgtgattg
     4201 tttattctat tactattgtt aaaaacttga atggtattta tattggctga tatttatata
     4261 cttaatagat tgagttctgt aagggcaaaa acggattgaa atgaacatag cgttttgcac
     4321 taatgataag ataacctgag aggtagggtt gaggaacaca gggttgaaag ctgttggaga
     4381 ggggaaaagt tgtgcataaa gaactgaaaa gggttctgga gtgttcattc agagtgaagg
     4441 gaaaacttta tccttctatt tcttataacc agacaggagg agttcttccc tttgaaaatt
     4501 gcaaaaggaa tgagaacttt gcagttggat gaatagagaa gggaaaagtt gctggagcac
     4561 ggagtgaaga tgggaggagc tgttagcaag gcccactgct gctcctgggt gagaagggag
     4621 ctctggagca ggaccagctg ggggccggca ctgctgctta gagtggagga ggctggccaa
     4681 agagactgtg ggctgttttc agtcagggag catgtgcatt gtttgtgctc aatttcagac
     4741 actcatttat aaacaaattc aaccaaggga tatttactgc tttatgttgc ttatgataat
     4801 tattttgcat taatacagta tacacatttg taaattcaac aggaaattat tgagttttgg
     4861 aaagctacag taatttctgt attacatcat ttatatgtga aaagttggac atttctgtaa
     4921 gtttttaaaa ttatccattt gttactttaa cattttaaaa ttatggtgtt ttcctgattt
     4981 taaaagcaat attttcctta ctgt
//
