LOCUS       NM_001013841            1376 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens signal transducing adaptor family member 2 (STAP2),
            transcript variant 2, mRNA.
ACCESSION   NM_001013841 XM_939590
VERSION     NM_001013841.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Sekine,Y., Kikkawa,K., Honda,S., Sasaki,Y., Kawahara,S.,
            Mizushima,A., Togi,S., Fujimuro,M., Oritani,K. and Matsuda,T.
  TITLE     STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl
            and regulates adipocyte differentiation
  JOURNAL   Sci Rep 14 (1), 5799 (2024)
   PUBMED   38461189
  REMARK    GeneRIF: STAP-2 facilitates insulin signaling through binding to
            CAP/c-Cbl and regulates adipocyte differentiation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Ichii,M., Oritani,K., Toda,J., Hosen,N., Matsuda,T. and Kanakura,Y.
  TITLE     Signal-transducing adaptor protein-1 and protein-2 in hematopoiesis
            and diseases
  JOURNAL   Exp Hematol 105, 10-17 (2022)
   PUBMED   34780812
  REMARK    GeneRIF: Signal-transducing adaptor protein-1 and protein-2 in
            hematopoiesis and diseases.
            Review article
REFERENCE   3  (bases 1 to 1376)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1376)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1376)
  AUTHORS   Kitai,Y., Iwakami,M., Saitoh,K., Togi,S., Isayama,S., Sekine,Y.,
            Muromoto,R., Kashiwakura,J.I., Yoshimura,A., Oritani,K. and
            Matsuda,T.
  TITLE     STAP-2 protein promotes prostate cancer growth by enhancing
            epidermal growth factor receptor stabilization
  JOURNAL   J Biol Chem 292 (47), 19392-19399 (2017)
   PUBMED   28986450
  REMARK    GeneRIF: High STAP2 expression is associated with prostate cancer.
REFERENCE   6  (bases 1 to 1376)
  AUTHORS   Sekine,Y., Yamamoto,T., Yumioka,T., Sugiyama,K., Tsuji,S.,
            Oritani,K., Shimoda,K., Minoguchi,M., Yoshimura,A. and Matsuda,T.
  TITLE     Physical and functional interactions between STAP-2/BKS and STAT5
  JOURNAL   J Biol Chem 280 (9), 8188-8196 (2005)
   PUBMED   15611091
  REMARK    GeneRIF: STAP-2/BKS is a modulator of STAT5-mediated signaling
REFERENCE   7  (bases 1 to 1376)
  AUTHORS   Yamamoto,T., Yumioka,T., Sekine,Y., Sato,N., Minoguchi,M.,
            Yoshimura,A. and Matsuda,T.
  TITLE     Regulation of FcepsilonRI-mediated signaling by an adaptor protein
            STAP-2/BSK in rat basophilic leukemia RBL-2H3 cells
  JOURNAL   Biochem Biophys Res Commun 306 (3), 767-773 (2003)
   PUBMED   12810085
  REMARK    GeneRIF: These data indicate that STAP-2/BKS negatively controls
            the FcepsilonRI-mediated calcium mobilization and degranulation by
            direct modulation of tyrosine phosphorylation of PLC-gamma.
REFERENCE   8  (bases 1 to 1376)
  AUTHORS   Minoguchi,M., Minoguchi,S., Aki,D., Joo,A., Yamamoto,T.,
            Yumioka,T., Matsuda,T. and Yoshimura,A.
  TITLE     STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation
            in acute-phase response through its YXXQ motif
  JOURNAL   J Biol Chem 278 (13), 11182-11189 (2003)
   PUBMED   12540842
REFERENCE   9  (bases 1 to 1376)
  AUTHORS   Mitchell,P.J., Sara,E.A. and Crompton,M.R.
  TITLE     A novel adaptor-like protein which is a substrate for the
            non-receptor tyrosine kinase, BRK
  JOURNAL   Oncogene 19 (37), 4273-4282 (2000)
   PUBMED   10980601
REFERENCE   10 (bases 1 to 1376)
  AUTHORS   Rosenzweig,B.L., Imamura,T., Okadome,T., Cox,G.N., Yamashita,H.,
            ten Dijke,P., Heldin,C.H. and Miyazono,K.
  TITLE     Cloning and characterization of a human type II receptor for bone
            morphogenetic proteins
  JOURNAL   Proc Natl Acad Sci U S A 92 (17), 7632-7636 (1995)
   PUBMED   7644468
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF677969.1, BC000795.1 and
            AC104521.3.
            
            On Jun 2, 2019 this sequence version replaced NM_001013841.1.
            
            Summary: This gene encodes the substrate of breast tumor kinase, an
            Src-type non-receptor tyrosine kinase. The encoded protein
            possesses domains and several tyrosine phosphorylation sites
            characteristic of adaptor proteins that mediate the interactions
            linking proteins involved in signal transduction pathways.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' coding region, compared to variant 1, resulting in a
            shorter protein (isoform 2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000795.1, SRR7346977.544763.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000594605.6/ ENSP00000471052.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BF677969.1         24-49
            27-350              BC000795.1         1-324
            351-352             AC104521.3         10808-10809         c
            353-1376            BC000795.1         327-1350
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1376
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /note="signal transducing adaptor family member 2"
                     /db_xref="GeneID:55620"
                     /db_xref="HGNC:HGNC:30430"
                     /db_xref="MIM:607881"
     exon            1..176
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1286
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /note="isoform 2 is encoded by transcript variant 2; brk
                     kinase substrate; breast tumor kinase substrate;
                     signal-transducing adaptor protein 2; BRK substrate"
                     /codon_start=1
                     /product="signal-transducing adaptor protein 2 isoform 2"
                     /protein_id="NP_001013863.1"
                     /db_xref="CCDS:CCDS45926.1"
                     /db_xref="GeneID:55620"
                     /db_xref="HGNC:HGNC:30430"
                     /db_xref="MIM:607881"
                     /translation="MASALRPPRVPKPKGVLPSHYYESFLEKKGPCDRDYKKFWAGLQ
                     GLTIYFYNSNRDFQHVEKLNLGAFEKLTDEIPWGSSRDPGTHFSLILRDQEIKFKVET
                     LECREMWKGFILTVVELRVPTDLTLLPGHLYMMSEVLAKEEARRALETPSCFLKVSRL
                     EAQLLLERYPECGNLLLRPSGDGADGVSVTTRQMHNGTHVVRHYKVKREGPKYVIDVE
                     QPFSCTSLDAVVNYFVSHTKKALVPFLLDEDYEKVLGYVEADKENGENVWVAPSAPGP
                     GPAPCTGGPKPLSPASSQDKLPPLPPLPNQEENYVTPIGDGPAVDYENQDVASSSWPV
                     ILKPKKLPKPPAKLPKPPVGPKPEPKVFNGGLGRKLPVSSAQPLFPTAGLADMTAELQ
                     KKLEKRRALEH"
     exon            177..248
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            249..371
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            372..428
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            429..529
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            530..664
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            665..734
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            735..837
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            838..903
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1053
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1146
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1221
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1376
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1356..1361
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /note="hexamer: ATTAAA"
     polyA_site      1376
                     /gene="STAP2"
                     /gene_synonym="BKS"
                     /note="major polyA site"
ORIGIN      
        1 ctgtttctca gcttcccggc ccagctgggg cacccactgg aaggagaggc caggcggaag
       61 accctggctc cgtcatggcc tctgccctga ggccaccccg tgtccccaag cctaagggtg
      121 tcctgccttc acactactat gagagctttc tagagaagaa ggggccctgt gaccgggatt
      181 acaagaagtt ctgggcaggc ctgcagggtc tcaccattta tttctacaat agcaatcggg
      241 acttccagca cgtggagaag ctcaacttgg gagcatttga gaaactcaca gatgagattc
      301 cctggggaag ctcacgtgac cctggcaccc acttcagcct gattctccgg gatcaggaga
      361 tcaagttcaa ggtagagacc ttggagtgtc gggaaatgtg gaaaggcttc atcttaacgg
      421 tggtggagct ccgtgtcccg accgacttga ccctgcttcc tgggcaccta tacatgatgt
      481 ctgaagtctt ggccaaagag gaggcgcgcc gtgcactgga gacaccctcg tgcttcctga
      541 aggtgagccg gctggaggca caactgctcc tggagcgcta ccccgagtgc gggaacctgc
      601 tgctgcggcc cagcggggac ggcgccgacg gcgtgtcggt caccacgcgg cagatgcaca
      661 acgggacgca cgtggtccgg cattacaagg tgaagcggga gggccccaag tacgtgatcg
      721 atgtggaaca gccgttctct tgcacctccc tggacgccgt ggtcaactat ttcgtgtcgc
      781 ataccaaaaa ggcgctggtg ccattcctgt tagacgagga ctacgagaag gtgctaggct
      841 acgtggaagc cgataaggag aatggcgaga atgtgtgggt ggcgccctcc gctccgggcc
      901 caggtcctgc accctgcaca ggtggcccca agccgctgtc acctgcgtct agccaggaca
      961 agctgccccc actgccccca ctaccgaacc aggaagagaa ctacgtgacc cccattggag
     1021 atggcccagc tgttgactat gagaaccaag atgtggcttc ctctagttgg ccagtcatcc
     1081 tgaagccaaa gaagttgcca aagcctcctg ccaagcttcc aaagccaccc gttggaccca
     1141 agccagagcc caaagtcttt aatggtggct tgggcaggaa gctgccagtc agttcagccc
     1201 agcctctctt ccccacagcc gggctggcag acatgacggc agagctacag aagaagctgg
     1261 agaagaggcg ggcactggag cactgattcg gacacaccag ggaccagcgg gctagtccca
     1321 gggcatggcc cagcggccag attctttttc ccaggattaa aactctgacc ccagga
//
