LOCUS       NM_005862               6062 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens STAG1 cohesin complex component (STAG1), mRNA.
ACCESSION   NM_005862
VERSION     NM_005862.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Huber,J., Tanasie,N.L., Zernia,S. and Stigler,J.
  TITLE     Single-molecule imaging reveals a direct role of CTCF's zinc
            fingers in SA interaction and cluster-dependent RNA recruitment
  JOURNAL   Nucleic Acids Res 52 (11), 6490-6506 (2024)
   PUBMED   38742641
  REMARK    GeneRIF: Single-molecule imaging reveals a direct role of CTCF's
            zinc fingers in SA interaction and cluster-dependent RNA
            recruitment.
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Alonso-Gil,D., Cuadrado,A., Gimenez-Llorente,D.,
            Rodriguez-Corsino,M. and Losada,A.
  TITLE     Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2
  JOURNAL   Nat Commun 14 (1), 1326 (2023)
   PUBMED   36898992
  REMARK    GeneRIF: Different NIPBL requirements of cohesin-STAG1 and
            cohesin-STAG2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6062)
  AUTHORS   Saitta,C., Rebellato,S., Bettini,L.R., Giudici,G., Panini,N.,
            Erba,E., Massa,V., Auer,F., Friedrich,U., Hauer,J., Biondi,A.,
            Fazio,G. and Cazzaniga,G.
  TITLE     Potential role of STAG1 mutations in genetic predisposition to
            childhood hematological malignancies
  JOURNAL   Blood Cancer J 12 (6), 88 (2022)
   PUBMED   35654786
  REMARK    GeneRIF: Potential role of STAG1 mutations in genetic
            predisposition to childhood hematological malignancies.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6062)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 6062)
  AUTHORS   Richart,L., Lapi,E., Pancaldi,V., Cuenca-Ardura,M., Pau,E.C.,
            Madrid-Mencia,M., Neyret-Kahn,H., Radvanyi,F., Rodriguez,J.A.,
            Cuartero,Y., Serra,F., Le Dily,F., Valencia,A., Marti-Renom,M.A.
            and Real,F.X.
  TITLE     STAG2 loss-of-function affects short-range genomic contacts and
            modulates the basal-luminal transcriptional program of bladder
            cancer cells
  JOURNAL   Nucleic Acids Res 49 (19), 11005-11021 (2021)
   PUBMED   34648034
  REMARK    GeneRIF: STAG2 loss-of-function affects short-range genomic
            contacts and modulates the basal-luminal transcriptional program of
            bladder cancer cells.
REFERENCE   6  (bases 1 to 6062)
  AUTHORS   Anazawa,Y., Arakawa,H., Nakagawa,H. and Nakamura,Y.
  TITLE     Identification of STAG1 as a key mediator of a p53-dependent
            apoptotic pathway
  JOURNAL   Oncogene 23 (46), 7621-7627 (2004)
   PUBMED   15361841
  REMARK    GeneRIF: STAG1, a novel transcriptional target for p53, mediates
            p53-dependent apoptosis, and might be a good candidate for
            next-generation gene therapy in cancer.
REFERENCE   7  (bases 1 to 6062)
  AUTHORS   Prieto,I., Pezzi,N., Buesa,J.M., Kremer,L., Barthelemy,I.,
            Carreiro,C., Roncal,F., Martinez,A., Gomez,L., Fernandez,R.,
            Martinez-A,C. and Barbero,J.L.
  TITLE     STAG2 and Rad21 mammalian mitotic cohesins are implicated in
            meiosis
  JOURNAL   EMBO Rep 3 (6), 543-550 (2002)
   PUBMED   12034751
REFERENCE   8  (bases 1 to 6062)
  AUTHORS   Gregson,H.C., Schmiesing,J.A., Kim,J.S., Kobayashi,T., Zhou,S. and
            Yokomori,K.
  TITLE     A potential role for human cohesin in mitotic spindle aster
            assembly
  JOURNAL   J Biol Chem 276 (50), 47575-47582 (2001)
   PUBMED   11590136
REFERENCE   9  (bases 1 to 6062)
  AUTHORS   Sumara,I., Vorlaufer,E., Gieffers,C., Peters,B.H. and Peters,J.M.
  TITLE     Characterization of vertebrate cohesin complexes and their
            regulation in prophase
  JOURNAL   J Cell Biol 151 (4), 749-762 (2000)
   PUBMED   11076961
REFERENCE   10 (bases 1 to 6062)
  AUTHORS   Carramolino,L., Lee,B.C., Zaballos,A., Peled,A., Barthelemy,I.,
            Shav-Tal,Y., Prieto,I., Carmi,P., Gothelf,Y., Gonzalez de
            Buitrago,G., Aracil,M., Marquez,G., Barbero,J.L. and Zipori,D.
  TITLE     SA-1, a nuclear protein encoded by one member of a novel gene
            family: molecular cloning and detection in hemopoietic organs
  JOURNAL   Gene 195 (2), 151-159 (1997)
   PUBMED   9305759
  REMARK    Erratum:[Gene 1998 Jan 12;206(2):283]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC064699.1, BC017735.1 and
            AC069524.18.
            
            On Nov 23, 2018 this sequence version replaced NM_005862.2.
            
            Summary: This gene is a member of the SCC3 family and is expressed
            in the nucleus. It encodes a component of cohesin, a multisubunit
            protein complex that provides sister chromatid cohesion along the
            length of a chromosome from DNA replication through prophase and
            prometaphase, after which it is dissociated in preparation for
            segregation during anaphase. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.23828.1,
                                           SRR7346977.2569678.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000383202.7/ ENSP00000372689.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3713              BC064699.1         1-3713
            3714-5141           BC017735.1         1089-2516
            5142-6062           AC069524.18        68193-69113
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.3"
     gene            1..6062
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /note="STAG1 cohesin complex component"
                     /db_xref="GeneID:10274"
                     /db_xref="HGNC:HGNC:11354"
                     /db_xref="MIM:604358"
     exon            1..184
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            185..296
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..4044
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /note="nuclear protein stromal antigen 1; SCC3 homolog 1;
                     sister chromatid cohesion 3 homolog A"
                     /codon_start=1
                     /product="cohesin subunit SA-1"
                     /protein_id="NP_005853.2"
                     /db_xref="CCDS:CCDS3090.1"
                     /db_xref="GeneID:10274"
                     /db_xref="HGNC:HGNC:11354"
                     /db_xref="MIM:604358"
                     /translation="MITSELPVLQDSTNETTAHSDAGSELEETEVKGKRKRGRPGRPP
                     STNKKPRKSPGEKSRIEAGIRGAGRGRANGHPQQNGEGEPVTLFEVVKLGKSAMQSVV
                     DDWIESYKQDRDIALLDLINFFIQCSGCRGTVRIEMFRNMQNAEIIRKMTEEFDEDSG
                     DYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAF
                     RHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKE
                     LQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKY
                     VGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVE
                     AIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEA
                     LAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLE
                     EPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNK
                     LTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVV
                     EKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEE
                     ADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQC
                     SHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDL
                     LMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEA
                     NKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQI
                     DKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTRE
                     AVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFL
                     TEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKR
                     VEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDF
                     LHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMS
                     SRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF"
     exon            297..399
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            400..564
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            565..661
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            662..738
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            739..943
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1095
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1169
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1293
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1392
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1472
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1580
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1695
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1813
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1917
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2010
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..2100
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2304
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2375
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2463
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2544
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2637
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2638..2812
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..2952
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            2953..3054
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3203
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3204..3332
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3538
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3713
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3714..3824
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3825..3939
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            3940..4020
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     exon            4021..6062
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4874..4879
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /note="hexamer: AATAAA"
     polyA_site      4900
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /note="major polyA site"
     regulatory      6044..6049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
                     /note="hexamer: AATAAA"
     polyA_site      6062
                     /gene="STAG1"
                     /gene_synonym="MRD47; SA1; SCC3A"
ORIGIN      
        1 attggcgtgt ggaaaatgcc accagatggc gggttaggat tgcagctccg ttgaaggcgc
       61 ggcccccgct cccgaacccc cggcgaccac cccgtaacaa cccccccaca tcgggaataa
      121 cacaccggag acttttgggg ggaaactagg tcgatggtcg gcggcgcccg gatgggcagc
      181 tgaggattgc ctttgaggtt attttaaaag ttttgagttg tacagcactt gattattttg
      241 ctgcattgtg aaaggacctc tccagcaatg attacttcag aattaccagt gttacaggat
      301 tcaactaatg aaactactgc ccattccgat gctggcagcg agcttgaaga aacagaggtc
      361 aaaggaaaaa gaaaaagggg tcgtcctggc cggcctccat ctacaaataa gaaacctcga
      421 aaatctccag gtgagaagag cagaattgaa gctggaatta gaggagcagg ccgtggaaga
      481 gctaatggac accctcaaca gaatggggaa ggggagcctg tcacattatt tgaggtggtg
      541 aaactgggga aaagtgcaat gcagtccgtg gtggatgact ggattgaatc atataaacaa
      601 gacagggaca tcgcacttct ggatttaatc aactttttta tccagtgttc aggatgtcga
      661 ggtactgtga gaatagagat gtttcgaaat atgcagaatg cagaaatcat cagaaaaatg
      721 actgaagaat ttgatgagga cagtggtgat tatcctctta ccatgcctgg acctcagtgg
      781 aaaaaatttc gttcaaactt ttgtgaattt attggagtcc tgattcgaca gtgtcagtat
      841 agcataattt atgatgagta tatgatggac acagtaatct cccttttgac gggtttgtca
      901 gactcccagg tcagagcttt taggcataca agtaccctgg ctgccatgaa gctcatgact
      961 gctctggtga atgttgcctt aaacctcagt attcatcagg ataataccca gagacaatat
     1021 gaagccgaga gaaataaaat gattgggaag agagccaatg aaaggttgga gttactactt
     1081 cagaaacgca aagagctgca agaaaatcag gatgaaatcg aaaatatgat gaactctatt
     1141 tttaagggta tatttgttca tagataccgt gatgctattg ctgagattag agccatttgt
     1201 attgaagaaa ttggagtatg gatgaaaatg tatagtgatg ccttcctaaa tgacagttac
     1261 ctaaaatatg ttggctggac tcttcatgac aggcaagggg aagtcaggct gaagtgtttg
     1321 aaagctctgc agagtctata taccaataga gaattattcc ccaaattgga actattcact
     1381 aaccgattca aggatcgcat tgtatcaatg acacttgata aagaatatga tgttgctgtg
     1441 gaagctattc gattggttac tctgatactt catggaagtg aagaagctct ttccaatgaa
     1501 gactgtgaaa atgtttacca cttggtgtac tcggcacatc gccctgttgc tgtggcagct
     1561 ggagagttcc ttcacaaaaa gctatttagc agacatgacc cacaagcaga agaagcatta
     1621 gcaaagagga ggggaagaaa cagcccgaat ggaaacctca ttaggatgct ggttcttttc
     1681 tttcttgaaa gtgagttaca tgaacatgca gcctacttgg tggacagttt atgggagagc
     1741 tctcaagaac tgttgaaaga ctgggaatgt atgacagagt tgctattaga agaacctgtt
     1801 caaggagagg aagcaatgtc tgatcgtcaa gagagtgctc ttatagagct aatggtttgt
     1861 acaattcgtc aagctgctga ggcacatcct ccagtgggaa ggggtaccgg caagagagtg
     1921 ctaactgcca aagaaaggaa aactcaaatt gatgatagaa acaaattgac tgaacatttt
     1981 attattacac ttcctatgtt actgtcaaag tattctgcag atgcagagaa ggtagcaaac
     2041 ttgctacaaa tcccacagta ttttgattta gaaatctaca gcacaggtag aatggaaaag
     2101 catctggatg ctttattaaa acagattaag tttgttgtgg agaaacacgt agaatcagat
     2161 gttctagaag cctgcagtaa aacctatagt atcttatgca gtgaagaata taccatccag
     2221 aacagagttg acatagctcg aagccagctg attgatgagt ttgtagatcg attcaatcat
     2281 tctgtggaag acctattgca agagggagaa gaagctgatg atgatgacat ttacaatgtt
     2341 ctttctacat taaagcggtt aacttctttt cacaatgcac atgatctcac aaaatgggat
     2401 ctctttggta attgctacag attattgaag actggaattg aacatggagc catgccagaa
     2461 cagatagtcg tgcaagcact gcagtgttcc cattattcga ttctttggca gttggtgaaa
     2521 attactgatg gctctccttc caaagaggat ttgttggtat tgaggaaaac ggtgaaatcc
     2581 tttttggctg tttgccagca gtgcctgtct aatgttaata ctccagtgaa agaacaggct
     2641 ttcatgttac tctgtgatct tctgatgatt ttcagccacc aattaatgac aggtggcaga
     2701 gagggccttc agcctttggt gttcaatcca gatactggac tccaatctga actcctcagt
     2761 tttgtgatgg atcacgtttt tattgaccaa gacgaggaga accagagcat ggagggtgat
     2821 gaagaagatg aagctaataa aattgaggcc ttacataaaa gaaggaatct acttgctgct
     2881 ttcagcaaac ttatcattta tgacattgtt gacatgcatg cagctgcaga catcttcaaa
     2941 cactacatga agtattacaa tgactatggt gatattatta aggaaacact gagtaaaacc
     3001 aggcagattg ataaaattca gtgtgccaag actctcattc tcagtttgca acagttattt
     3061 aatgaacttg ttcaagagca aggtcccaac ctagatagga catctgccca tgtcagtggc
     3121 attaaagaac tggcacgtcg ctttgccctt acatttggat tggaccagat taagacacga
     3181 gaagcagttg ccacacttca caaggatggc atagagtttg catttaaata ccaaaatcag
     3241 aaaggacaag agtatccacc tcctaatctg gcttttcttg aagtactaag tgaattttct
     3301 tctaaacttc ttcgacagga caaaaagaca gttcattcat acctagagaa attccttacc
     3361 gagcagatga tggaaaggag ggaggatgta tggcttccac tcatctccta tagaaattca
     3421 ttagtcactg ggggtgaaga tgatagaatg tctgtgaaca gtggaagtag cagcagcaaa
     3481 acctcatcag taaggaataa gaaaggacga cctccacttc ataaaaaacg agtagaagat
     3541 gagagtctgg ataacacatg gctaaacagg actgacacca tgattcagac tcctggcccc
     3601 ctgccagcac cacaactcac atccactgta ctgcgggaga acagtcggcc catgggagac
     3661 cagattcaag aacctgagtc tgaacatggt tctgaaccag actttttaca caatcctcag
     3721 atgcagatct cttggttagg ccagccgaag ttagaagact taaatcggaa ggacagaaca
     3781 ggaatgaact acatgaaagt gagaactgga gtgaggcatg ctgttcgggg tctaatggag
     3841 gaagatgctg agcccatctt tgaagatgtg atgatgtcat cccgaagcca gttagaagat
     3901 atgaatgaag aatttgagga caccatggtt attgatctgc ctccatcaag aaatcggcga
     3961 gagagagctg agctaaggcc agacttcttt gactctgcag ctatcataga agatgattca
     4021 ggatttggaa tgcctatgtt ctgaagtctg aagaaaattt acaaatctgg aactctatta
     4081 tttagagcta gaggcctata tactgtgata gcttgtatgg ggaaaaacac ttttgatgtg
     4141 atctgatttg ttttttaatc aaatgattaa ggtcaatccc tttttgcagt gacagaagag
     4201 gagcatgtaa attacccaag ggaatgttgg tgaatgtcaa ctcagaaaga ctgacctgaa
     4261 aatcatttgt gtcctactat tggacttatc ccaatacaga tgtgtgtgtt tttctggagg
     4321 gaggaagaaa ttttaaattt ttaaaacagc tgtcaagata aacactgtta tacacctgtt
     4381 ttatgaaaac tcaacattga gtaaaaaaaa acatattttt aactttattt tcctgttgta
     4441 caatttaaaa accgttttaa cattttgcct ttttatgttt taaaagctaa ccatttttat
     4501 taaacctatg agtaagcagc tcatcctaat tgcgaagagt gttttggagt tcactggatt
     4561 tggttgacct ttgtggaaca caaataatga aggagcagaa cattgacaag ctaagatgaa
     4621 attctgacat agtacatctc tgccaaaaac cacacaccct ctgtggatat ggatatgaat
     4681 tcccagattt tatatactct tgaataaaag gtttattttt atttataagt gggcataaaa
     4741 taagaaatgt ccatgcagcc atttttccaa cagatgctgt acaccgttca ttttatatag
     4801 actagggaga ttcaaataca gtgcattttc tattggtatt tgttctgtgc atttttagca
     4861 acttctacca gcaaataaag tattctcagt aaaacgaaaa tgattctcaa gttatcagtt
     4921 tgctgttttt accacttatt tcatgccctg ccaaattcaa gttacacaga cttccatttt
     4981 cttaagataa tcaatcatga agaaatcctt tatcaatcat tcaaaagtaa ttttaagtgt
     5041 aacataactg tgtttacttc ccatgcactt aataccctta tgcgctaatt ttgtgaatta
     5101 agtttactga ttatagaagt atgtgctgca tagaagtctg tgcttagagg gtgaagttcc
     5161 taagcttacc ttgaattaca gctacatttc agtgttaaat gtgcatatta agaataattc
     5221 ttttggggaa agaaattatg aatcttcagg acagtctaca atggtttaga gttacattct
     5281 gcctagactt ttatgacttg ctgctattgt tttaaaaacc ccacttagtc tctctcttcc
     5341 tttctgattt ctaaagtaag cctcagaatt tccaaaccaa ttcatccaca gctgtttctg
     5401 ggctggtttt taaagtagct gcaacagaat catgaggctt tcccttttta tcaaatacga
     5461 aaaacatttt ttaaaattct gcacacccag tgatcatctt ttgtgcggga aagcaagatg
     5521 atgatggatg attttattca tccttttagt aaagacacaa aacatttttc tcaacatttg
     5581 tacagttctg aaaaaaacct ggtcaccaaa aatatcttct ctgctaattc agcaattctt
     5641 gggctccagt taggggagct ggggcctcac tttctcccag aattgtgggc ttcactggaa
     5701 gtgaaggtgc aggaatgact ggactgtcca ccccagccct gcctgcctgt ggttttggcc
     5761 agggagcaag ccatgaggtg ccctggcaca tgcacaaatt gatcctttgc gtgacagtct
     5821 tgtatggaaa acagatgctg acagaattgt agactaccat gccacacaaa aaggctaaat
     5881 atctactcca atgggtttcc agttcagttt gaagtcaatc aaatttttgt attttcggtg
     5941 tctccttgat tggttttgct agtaattctg taaattgtac atttgcaata tgaggttttt
     6001 tttccttttg tacaatttga aactgatgct tcacctttcc tttaataaac tattcaaaat
     6061 ca
//
