LOCUS       NM_003034               9707 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ST8 alpha-N-acetyl-neuraminide
            alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 1,
            mRNA.
ACCESSION   NM_003034
VERSION     NM_003034.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Pilgrim,A.A., Jonus,H.C., Ho,A., Cole,A.C., Shim,J. and
            Goldsmith,K.C.
  TITLE     The yes-associated protein (YAP) is associated with resistance to
            anti-GD2 immunotherapy in neuroblastoma through downregulation of
            ST8SIA1
  JOURNAL   Oncoimmunology 12 (1), 2240678 (2023)
   PUBMED   37554309
  REMARK    GeneRIF: The yes-associated protein (YAP) is associated with
            resistance to anti-GD2 immunotherapy in neuroblastoma through
            downregulation of ST8SIA1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Yamamoto,Y., Higashimoto,K., Ohkawa,Y., Soejima,H., Kaneko,K.,
            Ohmi,Y., Furukawa,K. and Furukawa,K.
  TITLE     Possible regulation of ganglioside GD3 synthase gene expression
            with DNA methylation in human glioma cells
  JOURNAL   Glycoconj J 40 (3), 323-332 (2023)
   PUBMED   36897478
  REMARK    GeneRIF: Possible regulation of ganglioside GD3 synthase gene
            expression with DNA methylation in human glioma cells.
REFERENCE   3  (bases 1 to 9707)
  AUTHORS   Chopra,A., Mueller,R., Weiner,J. 3rd, Rosowski,J., Dommisch,H.,
            Grohmann,E. and Schaefer,A.S.
  TITLE     BACH1 Binding Links the Genetic Risk for Severe Periodontitis with
            ST8SIA1
  JOURNAL   J Dent Res 101 (1), 93-101 (2022)
   PUBMED   34160287
  REMARK    GeneRIF: BACH1 Binding Links the Genetic Risk for Severe
            Periodontitis with ST8SIA1.
REFERENCE   4  (bases 1 to 9707)
  AUTHORS   Zuo,F., Zhang,Y., Li,J., Yang,S. and Chen,X.
  TITLE     Long noncoding RNA NR2F1-AS1 plays a carcinogenic role in gastric
            cancer by recruiting transcriptional factor SPI1 to upregulate
            ST8SIA1 expression
  JOURNAL   Bioengineered 12 (2), 12345-12356 (2021)
   PUBMED   34738863
  REMARK    GeneRIF: Long noncoding RNA NR2F1-AS1 plays a carcinogenic role in
            gastric cancer by recruiting transcriptional factor SPI1 to
            upregulate ST8SIA1 expression.
REFERENCE   5  (bases 1 to 9707)
  AUTHORS   Xing,P., Wang,Y., Zhang,L., Ma,C. and Lu,J.
  TITLE     Knockdown of lncRNA MIR4435-2HG and ST8SIA1 expression inhibits the
            proliferation, invasion and migration of prostate cancer cells in
            vitro and in vivo by blocking the activation of the
            FAK/AKT/beta-catenin signaling pathway
  JOURNAL   Int J Mol Med 47 (6) (2021)
   PUBMED   33846784
  REMARK    GeneRIF: Knockdown of lncRNA MIR44352HG and ST8SIA1 expression
            inhibits the proliferation, invasion and migration of prostate
            cancer cells in vitro and in vivo by blocking the activation of the
            FAK/AKT/betacatenin signaling pathway.
REFERENCE   6  (bases 1 to 9707)
  AUTHORS   Nakayama,J., Fukuda,M.N., Hirabayashi,Y., Kanamori,A., Sasaki,K.,
            Nishi,T. and Fukuda,M.
  TITLE     Expression cloning of a human GT3 synthase. GD3 AND GT3 are
            synthesized by a single enzyme
  JOURNAL   J Biol Chem 271 (7), 3684-3691 (1996)
   PUBMED   8631981
REFERENCE   7  (bases 1 to 9707)
  AUTHORS   Haraguchi,M., Yamashiro,S., Yamamoto,A., Furukawa,K., Takamiya,K.,
            Lloyd,K.O., Shiku,H. and Furukawa,K.
  TITLE     Isolation of GD3 synthase gene by expression cloning of GM3
            alpha-2,8-sialyltransferase cDNA using anti-GD2 monoclonal antibody
  JOURNAL   Proc Natl Acad Sci U S A 91 (22), 10455-10459 (1994)
   PUBMED   7937974
REFERENCE   8  (bases 1 to 9707)
  AUTHORS   Nara,K., Watanabe,Y., Maruyama,K., Kasahara,K., Nagai,Y. and
            Sanai,Y.
  TITLE     Expression cloning of a CMP-NeuAc:NeuAc alpha 2-3Gal beta 1-4Glc
            beta 1-1'Cer alpha 2,8-sialyltransferase (GD3 synthase) from human
            melanoma cells
  JOURNAL   Proc Natl Acad Sci U S A 91 (17), 7952-7956 (1994)
   PUBMED   8058740
REFERENCE   9  (bases 1 to 9707)
  AUTHORS   Sasaki,K., Kurata,K., Kojima,N., Kurosawa,N., Ohta,S., Hanai,N.,
            Tsuji,S. and Nishi,T.
  TITLE     Expression cloning of a GM3-specific alpha-2,8-sialyltransferase
            (GD3 synthase)
  JOURNAL   J Biol Chem 269 (22), 15950-15956 (1994)
   PUBMED   8195250
REFERENCE   10 (bases 1 to 9707)
  AUTHORS   Sasaki,K., Watanabe,E., Kawashima,K., Sekine,S., Dohi,T.,
            Oshima,M., Hanai,N., Nishi,T. and Hasegawa,M.
  TITLE     Expression cloning of a novel Gal beta (1-3/1-4) GlcNAc alpha
            2,3-sialyltransferase using lectin resistance selection
  JOURNAL   J Biol Chem 268 (30), 22782-22787 (1993)
   PUBMED   7901202
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X77922.1, BC117285.1,
            AC007671.7 and BM929838.1.
            
            On Aug 11, 2020 this sequence version replaced NM_003034.3.
            
            Summary: Gangliosides are membrane-bound glycosphingolipids
            containing sialic acid. Ganglioside GD3 is known to be important
            for cell adhesion and growth of cultured malignant cells. The
            protein encoded by this gene is a type II membrane protein that
            catalyzes the transfer of sialic acid from CMP-sialic acid to GM3
            to produce gangliosides GD3 and GT3. The encoded protein may be
            found in the Golgi apparatus and is a member of glycosyltransferase
            family 29. Alternatively spliced transcript variants have been
            found for this gene. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.3107.1, X77922.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396037.9/ ENSP00000379353.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                X77922.1           8-81
            75-2042             BC117285.1         1-1968
            2043-9310           AC007671.7         5493-12760
            9311-9707           BM929838.1         1-397
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..9707
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /note="ST8 alpha-N-acetyl-neuraminide
                     alpha-2,8-sialyltransferase 1"
                     /db_xref="GeneID:6489"
                     /db_xref="HGNC:HGNC:10869"
                     /db_xref="MIM:601123"
     exon            1..711
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /inference="alignment:Splign:2.1.0"
     CDS             476..1546
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /EC_number="2.4.3.8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     disialoganglioside (GD3) synthase; sialyltransferase 8A
                     (alpha-N-acetylneuraminate: alpha-2,8-sialyltransferase,
                     GD3 synthase); ST8 alpha-N-acetylneuraminate
                     alpha-2,8-sialyltransferase 1; sialyltransferase 8
                     (alpha-N-acetylneuraminate: alpha-2,8-sialytransferase,
                     GD3 synthase) A; ganglioside-specific
                     alpha-2,8-polysialyltransferase; ganglioside GD3 synthase;
                     ganglioside GT3 synthase; alpha-N-acetylneuraminide
                     alpha-2,8-sialyltransferase; sialytransferase St8Sia I;
                     alpha-2,8-sialyltransferase 8A; sialyltransferase St8Sia
                     I"
                     /codon_start=1
                     /product="alpha-N-acetylneuraminide
                     alpha-2,8-sialyltransferase isoform 1"
                     /protein_id="NP_003025.1"
                     /db_xref="CCDS:CCDS8697.1"
                     /db_xref="GeneID:6489"
                     /db_xref="HGNC:HGNC:10869"
                     /db_xref="MIM:601123"
                     /translation="MSPCGRARRQTSRGAMAVLAWKFPRTRLPMGASALCVVVLCWLY
                     IFPVYRLPNEKEIVQGVLQQGTAWRRNQTAARAFRKQMEDCCDPAHLFAMTKMNSPMG
                     KSMWYDGEFLYSFTIDNSTYSLFPQATPFQLPLKKCAVVGNGGILKKSGCGRQIDEAN
                     FVMRCNLPPLSSEYTKDVGSKSQLVTANPSIIRQRFQNLLWSRKTFVDNMKIYNHSYI
                     YMPAFSMKTGTEPSLRVYYTLSDVGANQTVLFANPNFLRSIGKFWKSRGIHAKRLSTG
                     LFLVSAALGLCEEVAIYGFWPFSVNMHEQPISHHYYDNVLPFSGFHAMPEEFLQLWYL
                     HKIGALRMQLDPCEDTSLQPTS"
     exon            712..856
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /inference="alignment:Splign:2.1.0"
     exon            857..966
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1059
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..9707
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9689..9694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /note="hexamer: AATAAA"
     polyA_site      9707
                     /gene="ST8SIA1"
                     /gene_synonym="GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI"
                     /note="major polyA site"
ORIGIN      
        1 gttgcctggc ggcgcagcag cgcgggaggc ggcgaagggc gcaggagcat cgctcggagg
       61 ggacaagggg acgccacggg ccacatgttt aggagggagc cgagccttct cccggaccct
      121 cgccgagggc gaccgtgatg ctgcagaacc ggcgggagcg actcgccgcc gccgctctct
      181 gcgcactcgg agaccccagc gcccgccttc tgcaggggaa gcgaccatgg ccatagatcg
      241 tgacttcacc cccagccact tcccctagaa agaaatcctt ggaaaagttg catttgaaaa
      301 aatccttgcg ctgacctttg gggccgacgg ggccgaagaa gcgtgcgtgc gtttgcaagt
      361 aagagaacca aaggtgtgtg tgcatggggg gctggcggtg ggggaccctc cgctgccact
      421 tcgcctagct ttgtgctgag gccccggccc ccgcccctgg gacgccgggg ctgcgatgag
      481 cccctgcggg cgggcccggc gacaaacgtc cagaggggcc atggctgtac tggcgtggaa
      541 gttcccgcgg acccggctgc ccatgggagc cagtgccctc tgtgtcgtgg tcctctgttg
      601 gctctacatc ttccccgtct accggctgcc caacgagaaa gagatcgtgc agggggtgct
      661 gcaacagggc acggcgtgga ggaggaacca gaccgcggcc agagcgttca ggaaacaaat
      721 ggaagactgc tgcgaccctg cccatctctt tgctatgact aaaatgaatt cccctatggg
      781 gaagagcatg tggtatgacg gggagttttt atactcattc accattgaca attcaactta
      841 ctctctcttc ccacaggcaa ccccattcca gctgccattg aagaaatgcg cggtggtggg
      901 aaatggtggg attctgaaga agagtggctg tggccgtcaa atagatgaag caaattttgt
      961 catgcgatgc aatctccctc ctttgtcaag tgaatacact aaggatgttg gatccaaaag
     1021 tcagttagtg acagctaatc ccagcataat tcggcaaagg tttcagaacc ttctgtggtc
     1081 cagaaagaca tttgtggaca acatgaaaat ttataaccac agttacatct acatgcctgc
     1141 cttttctatg aagacaggaa cagagccatc tttgagggtt tattatacac tgtcagatgt
     1201 tggtgccaat caaacagtgc tgtttgccaa ccccaacttt ctgcgtagca ttggaaagtt
     1261 ctggaaaagt agaggaatcc atgccaagcg cctgtccaca ggactttttc tggtgagcgc
     1321 agctctgggt ctctgtgaag aggtggccat ctatggcttc tggcccttct ctgtgaatat
     1381 gcatgagcag cccatcagcc accactacta tgacaacgtc ttaccctttt ctggcttcca
     1441 tgccatgccc gaggaatttc tccaactctg gtatcttcat aaaatcggtg cactgagaat
     1501 gcagctggac ccatgtgaag atacctcact ccagcccact tcctaggaac aatggaagaa
     1561 gaaaggactg aaccagggta tttttgttag gttttctatg tgactccaag agggaatggt
     1621 caagttgttt catgagtttg catgggccct tggaaaaaca ggaaaggagc aatgaagatc
     1681 caagcaaaac tttactttca gcgttggctt ggaggacaaa taagaaatga aacatcctat
     1741 gaaatacttt atagcacatg gcagatttgc aactagtaaa atgctggtga aatgctgttg
     1801 gtaaagcaca tggttcaaat ctagaagatg cagttcaaaa acaagacaga ctcgagttgt
     1861 tagggctgag gaaccaatca aggtagaaca aagaaaatgt tggggtaaaa gtgttgctga
     1921 ttgtcaacac aaactggctt aataatatta ataagaacct gtcttattaa gactggcttt
     1981 agaaccgtag gtttttttaa aaaattatta tttatttttg ccctctttgg ggaagtgggt
     2041 gggtagattt aaaaaatccc ttcctgagta ataaagatac aaaatgttac tgctgataat
     2101 tgtgatttgt tgagccacgt ctatattaac tatagctccc ctctattttt aaaattttac
     2161 ataagaattg cttcttcctc ttttgtcaag tccacagaga caatgtgaag cattattgaa
     2221 tttagactgt gttgcttcat agtctggata acaggagcca ctttccttcc tcagaattta
     2281 ggacataaac ccaattttag gtctgcatta tgtaagcctg tttgcattgt catttttatt
     2341 ttagtgcaaa agactaccaa tatgagtgaa ttatttgttt gtaaattggc ataattgtct
     2401 tcataattta atgttcagaa atgcagtaaa gcacgttgat gaaaagctta gtctctgtgg
     2461 aagacagacc agaagacaaa tcccttttct atctactgtg gcatattcac tgcaaggtaa
     2521 aggacatgta tgcatacatg caagaatgaa ttgagcgaaa ctttgaatat gtttcctctt
     2581 ttgagctatt gttatttagt agttacctaa aaaagaatac atttttacta gtataccatt
     2641 aaaaatgtaa aaggcttaac atgtatgcat acatgtagac acacatacac acacacaaag
     2701 ttaaacaaag ctcattactg tgcaactatg aaagctgaac tcatatgacc tggtatggca
     2761 aaaatttgta accagtgttg gagaatcaca aatgtgttat gcactatcca ttacatgttg
     2821 atataagaca accgagctca ctggttgccg cacataatac ccatagcagc acaagatgca
     2881 agtcttaccc ccaaatgctt acccggaatg agcagaattg tcttactcat cctgatttta
     2941 cccaaaatct catgaatggg cttgctgttg agcgtggtca cacaaggtat agtttgtaca
     3001 agtaaagaaa tgtgaattag tagctattta tttccttagt agcaggtagg actacttagc
     3061 ttgttacaac agctttgata cacagtaaga aaactctact gacagattac aagcagccaa
     3121 atgtgtttac atttcagaga agggtaaggg aagcctctcg ttgtcttcat actttggaat
     3181 gtcttaagct aaaatgtgac actagagttc aatttctttt ttattttaaa tgtcactaaa
     3241 ttgtaggtaa actgtaccaa aaagttctag tttaatttta acagtaaaaa ctcagcatac
     3301 catgttacca cgtaaacaat aatggctggt attttctgtg ggttaattta acatgacata
     3361 aacataggaa agcccttggc aaaattctac atcattgaag tagaccattt ctaaatgcaa
     3421 ttaaatagtg aggttattat aaaaattaga atttaaaata tgacttgttg atagtgcaga
     3481 ttataatgtt acttagaaag ttaagaattc gagagtaatg atgctattgg agaactggtt
     3541 tgctatacgg cagcaaaaac ataatgagaa tgtaccatga agaaattgtg ttaaatattc
     3601 actttgagtg aaactttaaa tatgtttcct cttttgagct actgttattt agcagttacc
     3661 taaaaaagaa tacattttta ctagtctacc attaaaaata taaaaggctt aacaaaaacg
     3721 catataaaag ttaataaaat gttactcaga ttagtacttt aattgttaaa atgtaattaa
     3781 tgagctccat agagcccata catcacagtt ccattgaaaa tatcatctgg actagaatct
     3841 cttaaaccca ggaagtggag gttgcagtga gccaagatgg tgccactgca ctccagcctg
     3901 ggtgacaggg caagaccctg tctcaaaaaa aaaaaaaaga aaagaaaaag aaagaaaata
     3961 ttatgtggaa atttttcatc tttttatgat gcctgggata gatttatgca gtagatttat
     4021 atataatgtt tagtttacaa acttgtgaaa ctatttcatg tgggttctga aaggcaaaac
     4081 agaaatggcc agtgctggtg atgtttgatt agaccagctc ttctcagctg gggtgattca
     4141 ttatccctct ctggtgacat ttggcaatgt ctggagacca gagacatttt ttattttcat
     4201 gactcaaggg tgctaatagc atgtacttag tagaggttag ggatcctgct aaagatctta
     4261 caatgcacaa gtcagcctct cacagcaaaa taattatcca ggctgagatg tcagtagtat
     4321 caaggttgac atctcagacc tggattagac ctggattaga ccaaaatgaa attttgattg
     4381 ttccaccttg tcttcactta aagatagata tacatgctca cagcattcaa aatatgacta
     4441 caatttaatg aaaatatttt cttatggtag attacaactc actagataca ccatacatat
     4501 gccttttgga cataattggt tagataggag tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     4561 tgtgtgtgtg tgtgtagcat gaagttaggc atctctatcc ttaactgtgc tatgtgttcc
     4621 taagtaagga cctcgctaga atcatcctgt agagtgctct ggaatactct attattaaga
     4681 tcttcaattc taagctccca ttatctttct ttgtttatct gaaattaaga ccttcagaat
     4741 taggcctggt acttttccag atttgatcag ttacattttg atataactga ctcttaaaat
     4801 tcagcttaga agacattcaa taagaaaatc tttaatcact tttggtgatg cacctgccaa
     4861 gagggggcat cactttgtac catcagacta attaaaaaaa ttgttgtgta gtcataaaaa
     4921 ttaggctgat ccataggggc aaaaggaagg gtgaagagac taaaaactag taccctgata
     4981 gaattttagt ttttattagc ctcgatttga gaactgcatt gattccaaag atcatgaagg
     5041 taggtgaagg attaagtcat tccataatta agaaaagtaa tgtttatatg aaagattttt
     5101 atatggacaa tgactcacag acacatatgc tttgtataga ggattttgcc aaaagtttat
     5161 acaaaattgc acatctaaac tgcataattc tcatacaaga agttaaggaa gtgctcactg
     5221 gattttgctt ctgtatagtt cacttttatc tgtcttcaca agggtaggaa gtaaatgatc
     5281 tcaaatggca catgttcaga caatactgtg tttggctttg tgatgtctca tgtctttctc
     5341 ttggctaaaa aaaattgttt aggctatata taacttgtac ctgaagcaat ctggacaaca
     5401 tggcttaaaa tggaaaataa ctgaaaaaat cctgggattt taaatttgcc aaaaataccc
     5461 ttggattagt caaaggcttt acaagagtgt tatagatgat ccaaaatgga aacaatattg
     5521 ataacatttc ttttcgtcag cagctacata ttccagtttt ataactttat tatatacatt
     5581 ttttgattat taatagaaag tcagccatcc agagttttaa cttctgtggt tgaaatattg
     5641 actttgtgaa atttttcact gacaaatact tgttaggagg gacctaaggg ggaggggaat
     5701 gttaccagtc cactgaaaac tttaggaaat gttcattgtg ttgtgtaaat actttcccac
     5761 cagcaccagg taatatattc tctatgtttg tagaattcca aagatcaaaa gctgtaagga
     5821 cgtttatgtg aacttgacgc aaacttgtgg aggaataagg actgagagta atgttgtgct
     5881 ttttctttat cttcccgtcg tataggtcga ctctcaggtt atatgacttt tattgggcac
     5941 acgtcagggg tggaagtggt agaagtgggt taacaatttc aaggcctggc aacctaggtc
     6001 aaaacctcta ataaagtcag cagtaaacat aactagcaac ttcattccac tatgtgctag
     6061 gaggagggtg aggcaagttg aaaggagatg accctatccc cacactctca ggatgtaaaa
     6121 gggcagagtg ctctctaccc ttaaccttgg ctttttagca ccgtcagtct ctgtcctctc
     6181 ccaggagagc tgccttggct ccagagttga gctagtagca ttgtgactag agaggtagtg
     6241 tggaaattcg ctgaggacct caaaatttta cactgcctta aactgttttg tttttgctta
     6301 aactcgtgct gtctaaatgt gggtgttttg tggcagagtc tgatcacccc gacctagtta
     6361 tagccctgcc agtgattcta aagtggaact tttccccctc attcatatgg atagtctgag
     6421 tccttgaagc tagggagata tcaaatattc taccagttcc tacatagctt ctttaaaagt
     6481 cctagagact aactcacagg aagtctgaag cccagagcgt ctgtttataa actcctaaat
     6541 atgaggccac aatatttagg ttgagcacta gaaaatttct attttgcaag tggaaggaga
     6601 cttgaatcta taattgctgt agcttttttt cccttctatc ataagaaaat ggataccatc
     6661 tgctttaccc tcagcccaga cttgtctttt gaatttttta catacatctt aggggtgaga
     6721 gattgaaaca agtgcatgaa tgaaaagctt attgaaacat aacacagccc ctgctcacca
     6781 tttcagtaag ggaaaataca tgtttctgtt tccctctaat ttgttttctc tggtgccaac
     6841 atttctgatt tcttctgtct ctaatacata aggaataaat accatatttt aaattgactt
     6901 acatgcctgc gagcatttgc cagataatca aagcctatga ctctaacttt ttaaagcaca
     6961 gaagaggaat tatattttat ggtcactcct ctgtgtttct attgatctct atattgtgct
     7021 aaaattcttt caggtaattt ttttcttgtt gaagctgtgg gatataatca atgatgccaa
     7081 gctgtctgag aattttatag aataatatcc aaatatattg atcctgcctc tttctgcttc
     7141 tatgtaatta aactttcttt tgatgtttat ttttaaaatg tatgcttcac acaacaccaa
     7201 taaacatcta agagatgtga tataatccaa ggcaaagaaa agtaagaatc agttaatgtc
     7261 tattttatct gcacagcatt acgaacagaa aattgtaata aaaaaaggtt taaccaaact
     7321 gaaaaaaaga gagacaaaat cttggtttta atttgaaaag atgattggga gtgatctcag
     7381 aagagagttc agcaggaagc aaggaagagg ctgttgacct tgagtgcagc ctcaagaatg
     7441 gaaccttttt gaaatatttt aaaggaaata tcttgtcatc cagctgttat ggttaataat
     7501 gaatagtttt ctctgggtga agcttatcca ttgcacacct gatgtgctgg cccctgcgat
     7561 ttccccaaat gttggaatgt tgcctgcata atttgtggta atgggggact gtgttcctgc
     7621 ttttacctgg ttacaaaaaa caaaacaaaa caaaacaaaa caacaaacag aaaaaaaaag
     7681 agagaaaaga tgctagagga agcagtgtga cagacagatc atgagatgct gacggagaga
     7741 acagagtggt acctgtttac ccacttggac catgacagta tcaaggctcc accccttgct
     7801 gtcccttccc aagaggtgct ctcctgggag tttgatcaca gtgaaacaac actcccgttt
     7861 tctttttcct ttctttcttt ctttcttttt tttttttttt tttttttttg ttgagacaga
     7921 gttttttgct cttgttgccc aggctggagt gcaatggcgt gatctcggct caccgcaacc
     7981 tccgcctccc aggttcaagc gattctcgtg cctcagcctc tggagtagct gggattacag
     8041 gcatgcgcca ccacacccgg ctaattttgt acttttagta gagatggggt ttctccatat
     8101 tggtcaggct ggtcccgaac tcctgacctc aggtgatctg cccgcctcag cctcccaaag
     8161 tgctgggatt acaggcataa gccaccgagc ccagccccac actcctgttt tcatagccct
     8221 tttttgtctg tactttggta catattcccc aaataagatt attatttaca tctgtcatga
     8281 tgaggcagtg atagcagcaa gtgtcacttt gaccctcacc cttggaaagt gggctatgca
     8341 tagctagagt tagaacagga ggttctaatc ctgcttccac aattacttca gtaacaggct
     8401 tttgtatcca tgattactta agctgactta gatgcatttt ctaccctgta ttctagtgta
     8461 cacaacacaa acacacataa gccctttacc tgtggagaag agggagatgg ttgattcagt
     8521 gggagtgggg agtggcagag ggtgccttaa ctttttctgg cttgacccct acttccttat
     8581 gtgtttcctt catatacctc tgtcacttta gagatgtaat catgtctgtt ccaagttgta
     8641 tcgttattat caagaagtcc tataaatgta gcaaatgtga ctattttata gaaatgcaca
     8701 atagtttata ggccttggag aagtctgata atagtatgta aatatccttc attgaagtca
     8761 tagaaccttc tagtaggaag ctccataggt gataactgca agaggattaa tgtttgtttc
     8821 tccagtaaac accggaagca aggaatcaat ctgtcactgt ttatcattca gtcttgagca
     8881 ccgttgggtg ttgataggtt actatagaca ctccccttat aattaatata caggactcac
     8941 aatttaggat gtctgatggc cactagggta catcatagag acactagaga gaagagaaca
     9001 aatatagctc catcaaaaag cctgcttaat ctttaacaag tctgaaatat tactaaaatt
     9061 ccagttaccg tgcagcatag tcagtctaaa aagcactggg gaaaaaggtg tctttttggt
     9121 ctctttaatt ctattatggc acattttaat attattccac atgtgtgcaa actatttctc
     9181 aagagcaaag tttgaggctg gagttaagaa aatagaaaaa ttaaaacatt attttttctc
     9241 atataatata atctgactac tagttcatgg atataataat tctgggaaaa attttagatt
     9301 aaactataaa attgaaatca cctttgaaga gtatatggaa ttgcttcttt ctattaaagc
     9361 caacctacat tggcaaaaag tgtggagaaa atcagtgtgc tcaattcttt ttgtagagtt
     9421 tcatctaaaa tgttgcaggt aaaaaagaaa actttaattg gtactttcta cggtgtgatt
     9481 agaaaaatta gtatctatct ctgttggttc tgctgacttg cttctttcca ccgaatctaa
     9541 atgcaatgtc ataggaggag gtttgctgta aaaacatgtc tttttctttg gtgtattcat
     9601 tgtaattatg gctggcagtg agaaggttcg gtaagtctca atttctctac ctcctttact
     9661 tggagaaaat ttgtaaatgt accctgtgaa taaaatgatt ttttata
//
