LOCUS       NM_018414               2489 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ST6 N-acetylgalactosaminide
            alpha-2,6-sialyltransferase 1 (ST6GALNAC1), transcript variant 1,
            mRNA.
ACCESSION   NM_018414
VERSION     NM_018414.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Luo,Y., Cao,H., Lei,C. and Liu,J.
  TITLE     ST6GALNAC1 promotes the invasion and migration of breast cancer
            cells via the EMT pathway
  JOURNAL   Genes Genomics 45 (11), 1367-1376 (2023)
   PUBMED   37747641
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Yao,Y., Kim,G., Shafer,S., Chen,Z., Kubo,S., Ji,Y., Luo,J.,
            Yang,W., Perner,S.P., Kanellopoulou,C., Park,A.Y., Jiang,P., Li,J.,
            Baris,S., Aydiner,E.K., Ertem,D., Mulder,D.J., Warner,N.,
            Griffiths,A.M., Topf-Olivestone,C., Kori,M., Werner,L., Ouahed,J.,
            Field,M., Liu,C., Schwarz,B., Bosio,C.M., Ganesan,S., Song,J.,
            Urlaub,H., Oellerich,T., Malaker,S.A., Zheng,L., Bertozzi,C.R.,
            Zhang,Y., Matthews,H., Montgomery,W., Shih,H.Y., Jiang,J.,
            Jones,M., Baras,A., Shuldiner,A., Gonzaga-Jauregui,C.,
            Snapper,S.B., Muise,A.M., Shouval,D.S., Ozen,A., Pan,K.T., Wu,C.
            and Lenardo,M.J.
  TITLE     Mucus sialylation determines intestinal host-commensal homeostasis
  JOURNAL   Cell 185 (7), 1172-1188 (2022)
   PUBMED   35303419
  REMARK    GeneRIF: Mucus sialylation determines intestinal host-commensal
            homeostasis.
REFERENCE   3  (bases 1 to 2489)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   4  (bases 1 to 2489)
  AUTHORS   Lakshmanan,I., Chaudhary,S., Vengoji,R., Seshacharyulu,P.,
            Rachagani,S., Carmicheal,J., Jahan,R., Atri,P.,
            Chirravuri-Venkata,R., Gupta,R., Marimuthu,S., Perumal,N.,
            Rauth,S., Kaur,S., Mallya,K., Smith,L.M., Lele,S.M.,
            Ponnusamy,M.P., Nasser,M.W., Salgia,R., Batra,S.K. and Ganti,A.K.
  TITLE     ST6GalNAc-I promotes lung cancer metastasis by altering MUC5AC
            sialylation
  JOURNAL   Mol Oncol 15 (7), 1866-1881 (2021)
   PUBMED   33792183
  REMARK    GeneRIF: ST6GalNAc-I promotes lung cancer metastasis by altering
            MUC5AC sialylation.
REFERENCE   5  (bases 1 to 2489)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2489)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (bases 1 to 2489)
  AUTHORS   Donadio,S., Dubois,C., Fichant,G., Roybon,L., Guillemot,J.C.,
            Breton,C. and Ronin,C.
  TITLE     Recognition of cell surface acceptors by two human
            alpha-2,6-sialyltransferases produced in CHO cells
  JOURNAL   Biochimie 85 (3-4), 311-321 (2003)
   PUBMED   12770770
REFERENCE   8  (bases 1 to 2489)
  AUTHORS   Julien,S., Krzewinski-Recchi,M.A., Harduin-Lepers,A., Gouyer,V.,
            Huet,G., Le Bourhis,X. and Delannoy,P.
  TITLE     Expression of sialyl-Tn antigen in breast cancer cells transfected
            with the human CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase
            (ST6GalNac I) cDNA
  JOURNAL   Glycoconj J 18 (11-12), 883-893 (2001)
   PUBMED   12820722
  REMARK    GeneRIF: The stable transfection of MDA-MB-231 with an expression
            vector encoding ST6GalNAc I induces the expression of STn antigen
            at the cell surface.
REFERENCE   9  (bases 1 to 2489)
  AUTHORS   Ikehara,Y., Kojima,N., Kurosawa,N., Kudo,T., Kono,M., Nishihara,S.,
            Issiki,S., Morozumi,K., Itzkowitz,S., Tsuda,T., Nishimura,S.I.,
            Tsuji,S. and Narimatsu,H.
  TITLE     Cloning and expression of a human gene encoding an
            N-acetylgalactosamine-alpha2,6-sialyltransferase (ST6GalNAc I): a
            candidate for synthesis of cancer-associated sialyl-Tn antigens
  JOURNAL   Glycobiology 9 (11), 1213-1224 (1999)
   PUBMED   10536037
REFERENCE   10 (bases 1 to 2489)
  AUTHORS   Lee,Y.C., Kaufmann,M., Kitazume-Kawaguchi,S., Kono,M.,
            Takashima,S., Kurosawa,N., Liu,H., Pircher,H. and Tsuji,S.
  TITLE     Molecular cloning and functional expression of two members of mouse
            NeuAcalpha2,3Galbeta1,3GalNAc GalNAcalpha2,6-sialyltransferase
            family, ST6GalNAc III and IV
  JOURNAL   J Biol Chem 274 (17), 11958-11967 (1999)
   PUBMED   10207017
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA594489.1 and AY358918.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018414.4.
            
            Summary: Glycosylation of proteins affects cell-cell interaction,
            interactions with the matrix, and the functions of intracellular
            molecules. ST6GALNAC1 transfers a sialic acid, N-acetylneuraminic
            acid (NeuAc), in an alpha-2,6 linkage to O-linked GalNAc residues.
            The cancer-associated sialyl-Tn (sTn) antigen is formed by
            ST6GALNAC1-catalyzed sialylation of GalNAc residues on mucins
            (Ikehara et al., 1999 [PubMed 10536037]; Sewell et al., 2006
            [PubMed 16319059]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358918.1, SRR1660809.89494.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2156266
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000156626.12/ ENSP00000156626.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-484               DA594489.1         82-565
            485-2489            AY358918.1         511-2515
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..2489
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /note="ST6 N-acetylgalactosaminide
                     alpha-2,6-sialyltransferase 1"
                     /db_xref="GeneID:55808"
                     /db_xref="HGNC:HGNC:23614"
                     /db_xref="MIM:610138"
     exon            1..250
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..1922
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /EC_number="2.4.3.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sialyltransferase 7
                     ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,
                     3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase)
                     A; GalNAc alpha-2, 6-sialyltransferase I, long form;
                     alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase
                     1; SIAT7-A; ST6GalNAc I; sialyltransferase 7A; ST6
                     (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,
                     3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1;
                     ST6GALNAC1, alpha-2,6-sialyltransferase 1; ST6 GalNAc
                     alpha-2,6-sialyltransferase 1; galNAc
                     alpha-2,6-sialyltransferase I; hST6GalNAc-I"
                     /codon_start=1
                     /product="alpha-N-acetylgalactosaminide
                     alpha-2,6-sialyltransferase 1 isoform 1"
                     /protein_id="NP_060884.1"
                     /db_xref="CCDS:CCDS11748.1"
                     /db_xref="GeneID:55808"
                     /db_xref="HGNC:HGNC:23614"
                     /db_xref="MIM:610138"
                     /translation="MRSCLWRCRHLSQGVQWSLLLAVLVFFLFALPSFIKEPQTKPSR
                     HQRTENIKERSLQSLAKPKSQAPTRARRTTIYAEPVPENNALNTQTQPKAHTTGDRGK
                     EANQAPPEEQDKVPHTAQRAAWKSPEKEKTMVNTLSPRGQDAGMASGRTEAQSWKSQD
                     TKTTQGNGGQTRKLTASRTVSEKHQGKAATTAKTLIPKSQHRMLAPTGAVSTRTRQKG
                     VTTAVIPPKEKKPQATPPPAPFQSPTTQRNQRLKAANFKSEPRWDFEEKYSFEIGGLQ
                     TTCPDSVKIKASKSLWLQKLFLPNLTLFLDSRHFNQSEWDRLEHFAPPFGFMELNYSL
                     VQKVVTRFPPVPQQQLLLASLPAGSLRCITCAVVGNGGILNNSHMGQEIDSHDYVFRL
                     SGALIKGYEQDVGTRTSFYGFTAFSLTQSLLILGNRGFKNVPLGKDVRYLHFLEGTRD
                     YEWLEALLMNQTVMSKNLFWFRHRPQEAFREALHMDRYLLLHPDFLRYMKNRFLRSKT
                     LDGAHWRIYRPTTGALLLLTALQLCDQVSAYGFITEGHERFSDHYYDTSWKRLIFYIN
                     HDFKLEREVWKRLHDEGIIRLYQRPGPGTAKAKN"
     exon            251..950
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1119
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1291
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1430
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1534
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1624
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1724
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..2489
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2470..2475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /note="hexamer: AATAAA"
     polyA_site      2489
                     /gene="ST6GALNAC1"
                     /gene_synonym="HSY11339; SIAT7A; ST6GalNAcI; STYI"
                     /note="major polyA site"
ORIGIN      
        1 attgttaaga caaagggtgt gcacttcctg gccaggaaac ctgagcggtg agactcccag
       61 ctgcctacat caaggcccca ggacatgcag aaccttcctc tagaacccga cccaccacca
      121 tgaggtcctg cctgtggaga tgcaggcacc tgagccaagg cgtccagtgg tccttgcttc
      181 tggctgtcct ggtcttcttt ctcttcgcct tgccctcttt tattaaggag cctcaaacaa
      241 agccttccag gcatcaacgc acagagaaca ttaaagaaag gtctctacag tccctggcaa
      301 agcctaagtc ccaggcaccc acaagggcaa ggaggacaac catctatgca gagccagtgc
      361 cagagaacaa tgccctcaac acacaaaccc agcccaaggc ccacaccacc ggagacagag
      421 gaaaggaggc caaccaggca ccgccggagg agcaggacaa ggtgccccac acagcacaga
      481 gggcagcatg gaagagccca gaaaaagaga aaaccatggt gaacacactg tcacccagag
      541 ggcaagatgc agggatggcc tctggcagga cagaggcaca atcatggaag agccaggaca
      601 caaagacgac ccaaggaaat gggggccaga ccaggaagct gacggcctcc aggacggtgt
      661 cagagaagca ccagggcaaa gcggcaacca cagccaagac gctcattccc aaaagtcagc
      721 acagaatgct ggctcccaca ggagcagtgt caacaaggac gagacagaaa ggagtgacca
      781 cagcagtcat cccacctaag gagaagaaac ctcaggccac cccaccccct gcccctttcc
      841 agagccccac gacgcagaga aaccaaagac tgaaggccgc caacttcaaa tctgagcctc
      901 ggtgggattt tgaggaaaaa tacagcttcg aaataggagg ccttcagacg acttgccctg
      961 actctgtgaa gatcaaagcc tccaagtcgc tgtggctcca gaaactcttt ctgcccaacc
     1021 tcactctctt cctggactcc agacacttca accagagtga gtgggaccgc ctggaacact
     1081 ttgcaccacc ctttggcttc atggagctca actactcctt ggtgcagaag gtcgtgacac
     1141 gcttccctcc agtgccccag cagcagctgc tcctggccag cctccccgct gggagcctcc
     1201 ggtgcatcac ctgtgccgtg gtgggcaacg ggggcatcct gaacaactcc cacatgggcc
     1261 aggagataga cagtcacgac tacgtgttcc gattgagcgg agctctcatt aaaggctacg
     1321 aacaggatgt ggggactcgg acatccttct acggctttac cgccttctcc ctgacccagt
     1381 cactccttat attgggcaat cggggtttca agaacgtgcc tcttgggaag gacgtccgct
     1441 acttgcactt cctggaaggc acccgggact atgagtggct ggaagcactg cttatgaatc
     1501 agacggtgat gtcaaaaaac cttttctggt tcaggcacag accccaggaa gcttttcggg
     1561 aagccctgca catggacagg tacctgttgc tgcacccaga ctttctccga tacatgaaga
     1621 acaggtttct gaggtctaag accctggatg gtgcccactg gaggatatac cgccccacca
     1681 ctggggccct cctgctgctc actgcccttc agctctgtga ccaggtgagt gcttatggct
     1741 tcatcactga gggccatgag cgcttttctg atcactacta tgatacatca tggaagcggc
     1801 tgatctttta cataaaccat gacttcaagc tggagagaga agtctggaag cggctacacg
     1861 atgaagggat aatccggctg taccagcgtc ctggtcccgg aactgccaaa gccaagaact
     1921 gaccggggcc agggctgcca tggtctcctt gcctgctcca aggcacagga tacagtggga
     1981 atcttgagac tctttggcca tttcccatgg ctcagactaa gctccaagcc cttcaggagt
     2041 tccaagggaa cacttgaacc atggacaaga ctctctcaag atggcaaatg gctaattgag
     2101 gttctgaagt tcttcagtac attgctgtag gtcctgaggc cagggatttt taattaaatg
     2161 gggtgatggg tggccaatac cacaattcct gctgaaaaac actcttccag tccaaaagct
     2221 tcttgataca gaaaaaagag cctggattta cagaaacata tagatctggt ttgaattcca
     2281 gatcgagttt acagttgtga aatcttgaag gtattactta acttcactac agattgtcta
     2341 gaagaccttt ctaggagtta tctgattcta gaagggtcta tacttgtcct tgtctttaag
     2401 ctatttgaca actctacgtg ttgtagaaaa ctgataataa tacaaatgat tgttgtccat
     2461 ggaaaggcaa ataaattttc tacagtgaa
//
