LOCUS       NM_173344               6715 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 1
            (ST3GAL1), transcript variant 2, mRNA.
ACCESSION   NM_173344
VERSION     NM_173344.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Chen,F., Gao,K., Li,Y., Li,Y., Wu,Y., Dong,L., Yang,Z., Shi,J.,
            Guo,K., Gao,Q., Lu,H. and Zhang,S.
  TITLE     ST3GAL1 Promotes Malignant Phenotypes in Intrahepatic
            Cholangiocarcinoma
  JOURNAL   Mol Cell Proteomics 23 (9), 100821 (2024)
   PUBMED   39069074
  REMARK    GeneRIF: ST3GAL1 Promotes Malignant Phenotypes in Intrahepatic
            Cholangiocarcinoma.
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Zhang,N., Lin,S., Cui,W. and Newman,P.J.
  TITLE     Overlapping and unique substrate specificities of ST3GAL1 and 2
            during hematopoietic and megakaryocytic differentiation
  JOURNAL   Blood Adv 6 (13), 3945-3955 (2022)
   PUBMED   35507766
  REMARK    GeneRIF: Overlapping and unique substrate specificities of ST3GAL1
            and 2 during hematopoietic and megakaryocytic differentiation.
REFERENCE   3  (bases 1 to 6715)
  AUTHORS   Lin,W.D., Fan,T.C., Hung,J.T., Yeo,H.L., Wang,S.H., Kuo,C.W.,
            Khoo,K.H., Pai,L.M., Yu,J. and Yu,A.L.
  TITLE     Sialylation of CD55 by ST3GAL1 Facilitates Immune Evasion in Cancer
  JOURNAL   Cancer Immunol Res 9 (1), 113-122 (2021)
   PUBMED   33177111
  REMARK    GeneRIF: Sialylation of CD55 by ST3GAL1 Facilitates Immune Evasion
            in Cancer.
REFERENCE   4  (bases 1 to 6715)
  AUTHORS   Pietrobono,S., Anichini,G., Sala,C., Manetti,F., Almada,L.L.,
            Pepe,S., Carr,R.M., Paradise,B.D., Sarkaria,J.N., Davila,J.I.,
            Tofani,L., Battisti,I., Arrigoni,G., Ying,L., Zhang,C., Li,H.,
            Meves,A., Fernandez-Zapico,M.E. and Stecca,B.
  TITLE     ST3GAL1 is a target of the SOX2-GLI1 transcriptional complex and
            promotes melanoma metastasis through AXL
  JOURNAL   Nat Commun 11 (1), 5865 (2020)
   PUBMED   33203881
  REMARK    GeneRIF: ST3GAL1 is a target of the SOX2-GLI1 transcriptional
            complex and promotes melanoma metastasis through AXL.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6715)
  AUTHORS   Ortiz-Soto,M.E., Reising,S., Schlosser,A. and Seibel,J.
  TITLE     Structural and functional role of disulphide bonds and substrate
            binding residues of the human beta-galactoside
            alpha-2,3-sialyltransferase 1 (hST3Gal1)
  JOURNAL   Sci Rep 9 (1), 17993 (2019)
   PUBMED   31784620
  REMARK    GeneRIF: Structural and functional role of disulphide bonds and
            substrate binding residues of the human beta-galactoside
            alpha-2,3-sialyltransferase 1 (hST3Gal1).
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6715)
  AUTHORS   Vallejo-Ruiz,V., Haque,R., Mir,A.M., Schwientek,T., Mandel,U.,
            Cacan,R., Delannoy,P. and Harduin-Lepers,A.
  TITLE     Delineation of the minimal catalytic domain of human
            Galbeta1-3GalNAc alpha2,3-sialyltransferase (hST3Gal I)
  JOURNAL   Biochim Biophys Acta 1549 (2), 161-173 (2001)
   PUBMED   11690653
REFERENCE   7  (bases 1 to 6715)
  AUTHORS   Shang,J., Qiu,R., Wang,J., Liu,J., Zhou,R., Ding,H., Yang,S.,
            Zhang,S. and Jin,C.
  TITLE     Molecular cloning and expression of Galbeta1,3GalNAc alpha2,
            3-sialyltransferase from human fetal liver
  JOURNAL   Eur J Biochem 265 (2), 580-588 (1999)
   PUBMED   10504389
REFERENCE   8  (bases 1 to 6715)
  AUTHORS   Whitehouse,C., Burchell,J., Gschmeissner,S., Brockhausen,I.,
            Lloyd,K.O. and Taylor-Papadimitriou,J.
  TITLE     A transfected sialyltransferase that is elevated in breast cancer
            and localizes to the medial/trans-Golgi apparatus inhibits the
            development of core-2-based O-glycans
  JOURNAL   J Cell Biol 137 (6), 1229-1241 (1997)
   PUBMED   9182658
REFERENCE   9  (bases 1 to 6715)
  AUTHORS   Chang,M.L., Eddy,R.L., Shows,T.B. and Lau,J.T.
  TITLE     Three genes that encode human beta-galactoside alpha
            2,3-sialyltransferases. Structural analysis and chromosomal mapping
            studies
  JOURNAL   Glycobiology 5 (3), 319-325 (1995)
   PUBMED   7655169
REFERENCE   10 (bases 1 to 6715)
  AUTHORS   Kitagawa,H. and Paulson,J.C.
  TITLE     Differential expression of five sialyltransferase genes in human
            tissues
  JOURNAL   J Biol Chem 269 (27), 17872-17878 (1994)
   PUBMED   8027041
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN359425.1, AF059321.1,
            BX648001.1, BC018357.1 and AF186191.6.
            
            On Nov 22, 2018 this sequence version replaced NM_173344.2.
            
            Summary: The protein encoded by this gene is a type II membrane
            protein that catalyzes the transfer of sialic acid from CMP-sialic
            acid to galactose-containing substrates. The encoded protein is
            normally found in the Golgi but can be proteolytically processed to
            a soluble form. Correct glycosylation of the encoded protein may be
            critical to its sialyltransferase activity. This protein, which is
            a member of glycosyltransferase family 29, can use the same
            acceptor substrates as does sialyltransferase 4B. Two transcript
            variants encoding the same protein have been found for this gene.
            Other transcript variants may exist, but have not been fully
            characterized yet. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.4156.1,
                                           SRR1660809.129518.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN03267760
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000522652.6/ ENSP00000430515.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-252               CN359425.1         56-307
            253-1030            AF059321.1         4-781
            1031-1594           BX648001.1         1272-1835
            1595-2379           BC018357.1         1478-2262
            2380-6715           AF186191.6         83138-87473         c
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.22"
     gene            1..6715
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /note="ST3 beta-galactoside alpha-2,3-sialyltransferase 1"
                     /db_xref="GeneID:6482"
                     /db_xref="HGNC:HGNC:10862"
                     /db_xref="MIM:607187"
     exon            1..195
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            196..348
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            349..403
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            404..726
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            727..1082
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     CDS             777..1799
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /EC_number="2.4.3.4"
                     /EC_number="2.4.3.2"
                     /note="sialyltransferase 4A (beta-galactoside
                     alpha-2,3-sialyltransferase); sialyltransferase 4A
                     (beta-galactosidase alpha-2,3-sialytransferase);
                     sialyltransferase 4A (beta-galactoside
                     alpha-2,3-sialytransferase);
                     Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; SIAT4-A;
                     ST3GalI; alpha 2,3-ST 1; beta-galactoside
                     alpha-2,3-sialyltransferase 1; monosialoganglioside
                     sialyltransferase"
                     /codon_start=1
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2,3-sialyltransferase 1"
                     /protein_id="NP_775479.1"
                     /db_xref="CCDS:CCDS6373.1"
                     /db_xref="GeneID:6482"
                     /db_xref="HGNC:HGNC:10862"
                     /db_xref="MIM:607187"
                     /translation="MVTLRKRTLKVLTFLVLFIFLTSFFLNYSHTMVATTWFPKQMVL
                     ELSENLKRLIKHRPCTCTHCIGQRKLSAWFDERFNQTMQPLLTAQNALLEDDTYRWWL
                     RLQREKKPNNLNDTIKELFRVVPGNVDPMLEKRSVGCRRCAVVGNSGNLRESSYGPEI
                     DSHDFVLRMNKAPTAGFEADVGTKTTHHLVYPESFRELGDNVSMILVPFKTIDLEWVV
                     SAITTGTISHTYIPVPAKIRVKQDKILIYHPAFIKYVFDNWLQGHGRYPSTGILSVIF
                     SMHVCDEVDLYGFGADSKGNWHHYWENNPSAGAFRKTGVHDADFESNVTATLASINKI
                     RIFKGR"
     exon            1083..1279
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1459
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1505
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1625
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..6715
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6690..6695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /note="hexamer: AATAAA"
     polyA_site      6715
                     /gene="ST3GAL1"
                     /gene_synonym="1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA;
                     ST3GalA.1; ST3GalIA; ST3O"
                     /note="major polyA site"
ORIGIN      
        1 agacagtttc gacactttgc aaagacaaag agccctagac cggagggagg aggaggagga
       61 agaagagcgg agagagaagg aagaggcgat gtgagctggg aagggggcaa gtgtccggga
      121 cacccacacc cctgtattct cctccgaacc ccttcatgcc caaatcccgg aaactccagc
      181 gtgtctccag ccgtgttggt accattttca gatttcatct tcctaaactg gaaatgtcaa
      241 tgagaggaaa ttaacacccc caagagctgc agtgagcaaa tgcattgagc ttgggtcagg
      301 acaattccat ttggggacca gagatggacg gtcactcagc ctatggagat gaagaaactg
      361 aggttcagag aggttaagag actccactga ggtcacacag ccgatgacag acaaccttct
      421 gtgccttcat caagctggtt gtgtacccac catgtccctg gcgacaggat gggaaagaaa
      481 aagccctaat taaggatcgt cagaaaccac agttggagga ggacggcaga gacagtttcc
      541 ctccccgcta taccaacacc cttccttcga ggtcctcgct cctgagggac cctggactgt
      601 cacagagatt aatgacccct tatcttcttt ggatgtgaaa ggaaatcact ggttaaagct
      661 tgatcgagag acattatcag ctctttaagg attgcagaag aataggctac tttattttct
      721 gaaaaggagg gagttcctgc tacccatcgt gggaggccac catcaggact gcgaagatgg
      781 tgaccctgcg gaagaggacc ctgaaagtgc tcaccttcct cgtgctcttc atcttcctca
      841 cctccttctt cctgaactac tcccacacca tggtggccac cacctggttc cccaagcaga
      901 tggtcctgga gctctccgag aacctgaaga gactgatcaa gcacaggcct tgcacctgca
      961 cccactgcat cgggcagcgc aagctctcgg cctggttcga tgagaggttc aaccagacca
     1021 tgcagccgct gctgaccgcc cagaacgcgc tcttggagga cgacacctac cgatggtggc
     1081 tgaggctcca gcgggagaag aagcccaata acttgaatga caccatcaag gagctgttca
     1141 gagtggtgcc tgggaatgtg gaccctatgc tggagaagag gtcggtgggc tgccggcgct
     1201 gcgccgttgt gggcaactcg ggcaacctga gggagtcttc ttatgggcct gagatagaca
     1261 gtcacgactt tgtcctcagg atgaacaagg cgcccacggc agggtttgaa gctgatgttg
     1321 ggaccaagac cacccaccat ctggtgtacc ctgagagctt ccgggagctg ggagataatg
     1381 tcagcatgat cctggtgccc ttcaagacca tcgacttgga gtgggtggtg agcgccatca
     1441 ccacgggcac catttcccac acctacatcc cggttcctgc aaagatcaga gtgaaacagg
     1501 ataagatcct gatctaccac ccagccttca tcaagtatgt ctttgacaac tggctgcaag
     1561 ggcacgggcg atacccatct accggcatcc tctcggtcat cttctcaatg catgtctgcg
     1621 atgaggtgga cttgtacggc ttcggggcag acagcaaagg gaactggcac cactactggg
     1681 agaacaaccc atccgcgggg gcttttcgca agacgggggt gcacgatgca gactttgagt
     1741 ctaacgtgac ggccaccttg gcctccatca ataaaatccg gatcttcaag gggagatgac
     1801 gcagtgaagg gctgaggatg gacgcactgt cacacctctg catttccagc cccagcatct
     1861 tgctggagcc gttccatccc ggagcttgga ggggcagcct caggtgtgtg cctgggcacc
     1921 gctcacagcc tcttgcaccc agccgttggc agcatctact cagcaaggtc actaagctct
     1981 gccagcgtgg cagagcatgt cttggaacct gtcttgagtg gggacaacgt ccccccactg
     2041 ctgccctaga gctggggaga cgctgggaaa ggttcaacct ccacacacta aaatcatttt
     2101 ggctcctggg gcaagcttgg ggaatgaatg tggaagatgc ctatattctg agagacagga
     2161 cagtttccca ggaagatggg cagagacttg agtggcgatt acctccagca cagagacgtg
     2221 ccaggcggtg ttggcgctcg gggcgagatg ctgcccttct ttgcacgaag cctggcctct
     2281 tgcttggcgt gataaccctg tcatcttccc aaagctcatt tatgagccac cagaggctcc
     2341 taccccaaag attttcacag aaacttgagg ccaggtgccg tggctcacac ctgtaatctg
     2401 aacactttgg gaggccgagg cgggaggatc acttgagccc aggagttcaa gaccagcctg
     2461 ggcaacatag tgagactcct gtctctacaa aaataaaaga tttaaaaaaa ttagccaggc
     2521 acggtggcac acacttgtag ccccagctac tagggaggct gaggagggag gatctcttgt
     2581 gcctaggagt tcgaggctgc agtgggctgt gatcacacca ctgcactcca gcctgggcaa
     2641 cagagtgaga ccctgtctct gaaaaaaaaa aaaaaagaaa agaaaaaaaa ccccaaactt
     2701 ggcaccgtgt ttctgactcc tgcagatgcc tcttcccaag cagttgttcc tttgagtagt
     2761 cagtctcatg gttctaatcg tggcgagagc ggatctgacc acttccatgt gcctttgtat
     2821 ttgggaaaga aaagcatgca ttggctgaaa tgaaacaaaa gcccttactt gggaaaaggg
     2881 acccccacca gcggccctgc ttgctccgta gctgggaaca tcttttctgg tttcctgagc
     2941 cagcacctga attgcaacga agctctgagg atactcaaca ccacccagca gtgcgtcaga
     3001 atggaacgta cattctcatt ctcagactgt tcaatatggt gcccgtaacc tccaaagcca
     3061 cacaggaggc tgccagcagc attttcccca agggcctgag ctcagggtca agtccctgct
     3121 ggccatcttg gggctgtcac tgggggtctg ggtcaaggac atcccaggaa tggtaactgc
     3181 cacatacctc atcttcttac tccccagcta ctctcttttc ctcattagcc ttcctgatta
     3241 tttattgatt tctttttttt attcaactga cattgtttgc accctggctc tgttggtggt
     3301 cctggtgttt taatgaagct tgcctcaccc agtagctggg aaattaggtg ggggtgctta
     3361 agggaggttt gtaggagtcc tcggacgtgg gatattgggg gtatctaggg cttggatggt
     3421 ttgtggaatg tcctcagcat cctcccgtcc caacccaacc ttccttcttc ctcattgtgg
     3481 acttggattt attctcaggc taggagtctc caaaactccg tgaaacctta ttgctgctgg
     3541 gtgtggagtg gagagtgaca ggaatgtggg ggacggtgat tgttggctcc agttactaat
     3601 gtttaagcag aaaaaccact ttcttgttga aaatatccct gaaaaggtcg ctgctgcttt
     3661 gaggaggcgt gactttttcc tggtggagtg tgacggaggc atggggcctg tgctcccagg
     3721 ctcgggggag ccccctctgc acacggggag ggaggaactt ggggacatca gtcatgggtc
     3781 acaggtggct tcaggccatt cacaagagtc cccggaagtt gcgtgccaag tttggttttt
     3841 tccctgaatc tctgcatatg agcatttttc tggagaaagg accatagctt ttattagatt
     3901 ctcaaaggtt cagaatgggg aatgtgttaa acaaaccgaa aacctgtttc agctttaaaa
     3961 tcctccaaaa ctttaaagac ttttcttttt tctgagaaag gctggtaaat cactgagtca
     4021 tcattcagaa catacagcct cgaagggcag agctcacacc catatatgac gttaagagct
     4081 caacaaatgt ttgttgagtg actgagtgac cccaggggca gactccagat catcagacac
     4141 tgatacagag agcgatgcta tctcctcaac tctccctctt ctcctaaaag ctgagtctat
     4201 atagggatga caggctcagg ctgagcagtc gacttctctg ggttttgctg tgtaagaata
     4261 ggtccctcaa catgaagatg tgtctgcgtc tgagccagat gctgcctggg tcggttgcct
     4321 ggcctccgtc tggtctccct ccctcctact agggtggtca agctgtgaga tctcttccag
     4381 cctccaaatc cagctttccc attttactga tgagaaaatc aaggccacat tgcagctggg
     4441 tggggagtgg agcgtgacag gcacgggaga ggacagcgat tgttgactct agttcctgat
     4501 gtttaatcag aaaaaccact tttcctgtag agcacatttc ctaaaaggct gctgctgtgt
     4561 agggaggtgt ggccttttcc tggtgcagtg tgaaggaggg aagtgacggg cagtcgtgaa
     4621 ctgtctggga cagatagcac aagtcagagt ttgagcaaaa ctagggtgtt accggcgcta
     4681 tgttgaaatt gtctggaggc gggctttccc agcgagtctc cattcagcat ctggaaaaag
     4741 tgtaaccaga atggaggtgt ctgactctcc tgtgctctct cagcgccgag ctctagctgc
     4801 aggctgcctg ggtggccttc tcacaccctc ccgtgcagga gactgcattt ctctttccca
     4861 cagcccagca gatagctcat tggcttagca atgggcactt aacagaatcc agttgttgca
     4921 gccagaatta aatgcaccag tctggcttaa accaactgga actcatccct ggggtgaagg
     4981 tggctgggaa ttagacaaga gtgtgtgtta gccaaaaggc acatgcatgc tgtcagcagg
     5041 agaatgagga gcactggttc tgggcaggca gtggcagggg ctccctcagg cccctgattc
     5101 ccagcccaca gctctttcta ctctgcagac agagttcctt ggagactggc cctgcattca
     5161 ggcagggagc agaggggtcc ctgcctgcag cacccagagc tgaacctgaa gctacaaaga
     5221 tgggctgatg aggtggccca tgaggtgtca gcaacatccc aaccccctgg gtctccccag
     5281 tgagtcaggg cactgatttg ctatagctgg agctacatgc acctctcctc ccctcagcca
     5341 ccttttaggg gagaaaggag ggcagatttg taacctctgg ttctctgcag ggatcctggg
     5401 ctggaagaaa gttcataaat ggatgaatgc cttttaaaac tccagctttt ccaagcatca
     5461 ttcaccaaag catttggtta ggcttagaga gaaaagctgg atatgagaaa atagaatgta
     5521 aaataggagg gaaaaaaaaa aagaaggtgg agagggcagt taagatctgg gtgattcttt
     5581 agggaaacgg ctggttgaga ggctttgatg gaatcccatt ctctgctgtg gccagaaggt
     5641 ggtgctagag gccagtccca cttggatgtg accctggcct ctgccaggat tcaaaccaca
     5701 aagaacattt ggaactggga tgtttgcttt gaagcagccc gaaaacaagt gaaaggaaac
     5761 caagataact ctctctccct ccgctggcaa ggatcaaaaa taccttgtgc agggaagccc
     5821 gagcctcgag gggccactgc ccagctaaag atgactggac accagccatg caccatcagg
     5881 aaccagcacc tgtcctcacc cctgagaaga gccccggggg tatcattata tccatcgcag
     5941 acatctggtt tgcagcaaat gtggctacac gccatcagga ttatcctgcc ctgtggactg
     6001 ggctagctag ttttgagata gagtcaacct ccccattttt gtttgtcatg aaaacaccag
     6061 ctcctgcacc cctggggtgc tacctgacac ctgtgcaaag aaaaggagga aaacttagaa
     6121 gaaaaggcag agacaggcaa gacagcatct cacagcttgt caggatcagg gcattaccga
     6181 ctattttttc ttaccaaaag agcactgaat ttcctagggg aaggtgagcc acctttacac
     6241 ctcctgtcct ccctcccagc ccgtcaagcg atcccatcac ccagcctcat cactcagctt
     6301 cttcctagta caaaactgcc atcggttgta cgtcctgagc tgtgggacgt cagctttaac
     6361 cctagcctgt ctcagggcct cgccctccag ccccccgccc cttagggtga tcgtgggttt
     6421 cttcccttcc tcctgcacaa tcgcttccct tcactgaatt cccttcaaag ccaaagtttc
     6481 ttgagcactt tttcttgtgt gcagtcttgt gtgtttgggt gttggtgttt ttaaatttgc
     6541 tttctattcc cccctgcctc tctccatgtc gttacgcagc tgagcactgg ttgggatgca
     6601 ttcgaatgac ttcagcaaga tcctgctttc ctcagccccc gctctgtttt atttattgtt
     6661 taatatttcc aatgatccaa atcaaagtga ataaacaaag agctatttta ttgtt
//
