LOCUS       NM_003146               2831 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens structure specific recognition protein 1 (SSRP1),
            mRNA.
ACCESSION   NM_003146
VERSION     NM_003146.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Lyu,Y., Yang,Y., Talwar,V., Lu,H., Chen,C., Salman,S., Wicks,E.E.,
            Huang,T.Y., Drehmer,D., Wang,Y., Zuo,Q., Datan,E., Jackson,W. 3rd,
            Dordai,D., Wang,R. and Semenza,G.L.
  TITLE     Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate
            histone ubiquitination and transcriptional activation of target
            genes
  JOURNAL   Cell Rep 43 (4), 113972 (2024)
   PUBMED   38517892
  REMARK    GeneRIF: Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to
            mediate histone ubiquitination and transcriptional activation of
            target genes.
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Chen,X., Li,M., Wang,D., Wang,Q., Wei,X., Liu,X., Yang,J.,
            Kalvakolanu,D.V., Guo,B. and Zhang,L.
  TITLE     Histone chaperone SSRP1 is required for apoptosis inhibition and
            mitochondrial function in HCC via transcriptional promotion of
            TRAP1
  JOURNAL   Biochem Cell Biol 101 (4), 361-376 (2023)
   PUBMED   37084412
  REMARK    GeneRIF: Histone chaperone SSRP1 is required for apoptosis
            inhibition and mitochondrial function in HCC via transcriptional
            promotion of TRAP1.
REFERENCE   3  (bases 1 to 2831)
  AUTHORS   McCauley,M.J., Morse,M., Becker,N., Hu,Q., Botuyan,M.V.,
            Navarrete,E., Huo,R., Muthurajan,U.M., Rouzina,I., Luger,K.,
            Mer,G., Maher,L.J. 3rd and Williams,M.C.
  TITLE     Human FACT subunits coordinate to catalyze both disassembly and
            reassembly of nucleosomes
  JOURNAL   Cell Rep 41 (13), 111858 (2022)
   PUBMED   36577379
  REMARK    GeneRIF: Human FACT subunits coordinate to catalyze both
            disassembly and reassembly of nucleosomes.
REFERENCE   4  (bases 1 to 2831)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 2831)
  AUTHORS   Sivkina,A.L., Karlova,M.G., Valieva,M.E., McCullough,L.L.,
            Formosa,T., Shaytan,A.K., Feofanov,A.V., Kirpichnikov,M.P.,
            Sokolova,O.S. and Studitsky,V.M.
  TITLE     Electron microscopy analysis of ATP-independent nucleosome
            unfolding by FACT
  JOURNAL   Commun Biol 5 (1), 2 (2022)
   PUBMED   35013515
  REMARK    GeneRIF: Electron microscopy analysis of ATP-independent nucleosome
            unfolding by FACT.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2831)
  AUTHORS   Dyer,M.A., Hayes,P.J. and Baron,M.H.
  TITLE     The HMG domain protein SSRP1/PREIIBF is involved in activation of
            the human embryonic beta-like globin gene
  JOURNAL   Mol Cell Biol 18 (5), 2617-2628 (1998)
   PUBMED   9566881
REFERENCE   7  (bases 1 to 2831)
  AUTHORS   Orphanides,G., LeRoy,G., Chang,C.H., Luse,D.S. and Reinberg,D.
  TITLE     FACT, a factor that facilitates transcript elongation through
            nucleosomes
  JOURNAL   Cell 92 (1), 105-116 (1998)
   PUBMED   9489704
REFERENCE   8  (bases 1 to 2831)
  AUTHORS   Nagulapalli,S., Pongubala,J.M. and Atchison,M.L.
  TITLE     Multiple proteins physically interact with PU.1. Transcriptional
            synergy with NF-IL6 beta (C/EBP delta, CRP3)
  JOURNAL   J Immunol 155 (9), 4330-4338 (1995)
   PUBMED   7594592
REFERENCE   9  (bases 1 to 2831)
  AUTHORS   Bruhn,S.L., Pil,P.M., Essigmann,J.M., Housman,D.E. and Lippard,S.J.
  TITLE     Isolation and characterization of human cDNA clones encoding a high
            mobility group box protein that recognizes structural distortions
            to DNA caused by binding of the anticancer agent cisplatin
  JOURNAL   Proc Natl Acad Sci U S A 89 (6), 2307-2311 (1992)
   PUBMED   1372440
REFERENCE   10 (bases 1 to 2831)
  AUTHORS   Toney,J.H., Donahue,B.A., Kellett,P.J., Bruhn,S.L., Essigmann,J.M.
            and Lippard,S.J.
  TITLE     Isolation of cDNAs encoding a human protein that binds selectively
            to DNA modified by the anticancer drug
            cis-diamminedichloroplatinum(II)
  JOURNAL   Proc Natl Acad Sci U S A 86 (21), 8328-8332 (1989)
   PUBMED   2530581
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1990 Feb;87(4):1625]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000781.6.
            
            On Nov 26, 2018 this sequence version replaced NM_003146.2.
            
            Summary: The protein encoded by this gene is a subunit of a
            heterodimer that, along with SUPT16H, forms chromatin
            transcriptional elongation factor FACT. FACT interacts specifically
            with histones H2A/H2B to effect nucleosome disassembly and
            transcription elongation. FACT and cisplatin-damaged DNA may be
            crucial to the anticancer mechanism of cisplatin. This encoded
            protein contains a high mobility group box which most likely
            constitutes the structure recognition element for
            cisplatin-modified DNA. This protein also functions as a
            co-activator of the transcriptional activator p63. An alternatively
            spliced transcript variant of this gene has been described, but its
            full-length nature is not known. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853562.5331.1,
                                           SRR11853560.5239.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000278412.7/ ENSP00000278412.2
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-163               AP000781.6         91047-91209         c
            164-336             AP000781.6         90385-90557         c
            337-522             AP000781.6         89780-89965         c
            523-628             AP000781.6         88752-88857         c
            629-819             AP000781.6         88276-88466         c
            820-1050            AP000781.6         87942-88172         c
            1051-1154           AP000781.6         87700-87803         c
            1155-1283           AP000781.6         87469-87597         c
            1284-1505           AP000781.6         86985-87206         c
            1506-1578           AP000781.6         86172-86244         c
            1579-1717           AP000781.6         85608-85746         c
            1718-1763           AP000781.6         85410-85455         c
            1764-1893           AP000781.6         83614-83743         c
            1894-2064           AP000781.6         83029-83199         c
            2065-2153           AP000781.6         82743-82831         c
            2154-2340           AP000781.6         82020-82206         c
            2341-2831           AP000781.6         81305-81795         c
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..2831
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /note="structure specific recognition protein 1"
                     /db_xref="GeneID:6749"
                     /db_xref="HGNC:HGNC:11327"
                     /db_xref="MIM:604328"
     exon            1..163
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            164..336
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..2412
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /note="chromatin-specific transcription elongation factor
                     80 kDa subunit; high mobility group box; facilitates
                     chromatin remodeling 80 kDa subunit; cisplatin-DNA SSRP;
                     hSSRP1; FACTp80; FACT 80 kDa subunit; recombination signal
                     sequence recognition protein 1; facilitates chromatin
                     transcription complex subunit SSRP1; facilitates chromatin
                     transcription complex 80 kDa subunit"
                     /codon_start=1
                     /product="FACT complex subunit SSRP1"
                     /protein_id="NP_003137.1"
                     /db_xref="CCDS:CCDS7952.1"
                     /db_xref="GeneID:6749"
                     /db_xref="HGNC:HGNC:11327"
                     /db_xref="MIM:604328"
                     /translation="MAETLEFNDVYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQ
                     AGELTEGIWRRVALGHGLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDL
                     CVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSL
                     MEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRI
                     YPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILL
                     FSKDEDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQG
                     HSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFE
                     IETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHD
                     AYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEG
                     DSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIK
                     SDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKR
                     DKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKR
                     RRSEDSEEEELASTPPSSEDSASGSDE"
     exon            337..522
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            523..628
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            629..819
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            820..1050
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1154
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1283
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1505
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1578
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1717
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1763
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1893
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2064
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2153
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            2154..2340
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     exon            2341..2831
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2808..2813
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /note="hexamer: AATAAA"
     polyA_site      2831
                     /gene="SSRP1"
                     /gene_synonym="FACT; FACT80; T160"
                     /note="major polyA site"
ORIGIN      
        1 ctctctgtgg cctgtgtacg gcttccggtg gcgggacgcg gggccgcgca cgcgggaaaa
       61 gcttccccgg tgtcccccca tccccctccc cgcgcccccc ccgcgtcccc ccagcgcgcc
      121 cacctctcgc gccggggccc tcgcgaggcc gcagcctgag gagattccca acctgctgag
      181 catccgcaca cccactcagg agttggggcc cagctcccag tttacttggt ttcccttgtg
      241 cagcctgggg ctctgcccag gccaccacag gcaggggtcg acatggcaga gacactggag
      301 ttcaacgacg tctatcagga ggtgaaaggt tccatgaatg atggtcgact gaggttgagc
      361 cgtcagggca tcatcttcaa gaatagcaag acaggcaaag tggacaacat ccaggctggg
      421 gagttaacag aaggtatctg gcgccgtgtt gctctgggcc atggacttaa actgcttaca
      481 aagaatggcc atgtctacaa gtatgatggc ttccgagaat cggagtttga gaaactctct
      541 gatttcttca aaactcacta tcgccttgag ctaatggaga aggacctttg tgtgaagggc
      601 tggaactggg ggacagtgaa atttggtggg cagctgcttt cctttgacat tggtgaccag
      661 ccagtctttg agatacccct cagcaatgtg tcccagtgca ccacaggcaa gaatgaggtg
      721 acactggaat tccaccaaaa cgatgacgca gaggtgtctc tcatggaggt gcgcttctac
      781 gtcccaccca cccaggagga tggtgtggac cctgttgagg cctttgccca gaatgtgttg
      841 tcaaaggcgg atgtaatcca ggccacggga gatgccatct gcatcttccg ggagctgcag
      901 tgtctgactc ctcgtggtcg ttatgacatt cggatctacc ccacctttct gcacctgcat
      961 ggcaagacct ttgactacaa gatcccctac accacagtac tgcgtctgtt tttgttaccc
     1021 cacaaggacc agcgccagat gttctttgtg atcagcctgg atcccccaat caagcaaggc
     1081 caaactcgct accacttcct gatcctcctc ttctccaagg acgaggacat ttcgttgact
     1141 ctgaacatga acgaggaaga agtggagaag cgctttgagg gtcggctcac caagaacatg
     1201 tcaggatccc tctatgagat ggtcagccgg gtcatgaaag cactggtaaa ccgcaagatc
     1261 acagtgccag gcaacttcca agggcactca ggggcccagt gcattacctg ttcctacaag
     1321 gcaagctcag gactgctcta cccgctggag cggggcttca tctacgtcca caagccacct
     1381 gtgcacatcc gcttcgatga gatctccttt gtcaactttg ctcgtggtac cactactact
     1441 cgttcctttg actttgaaat tgagaccaag cagggcactc agtatacctt cagcagcatt
     1501 gagagggagg agtacgggaa actgtttgat tttgtcaacg cgaaaaagct caacatcaaa
     1561 aaccgaggat tgaaagaggg catgaaccca agctacgatg aatatgctga ctctgatgag
     1621 gaccagcatg atgcctactt ggagaggatg aaggaggaag gcaagatccg ggaggagaat
     1681 gccaatgaca gcagcgatga ctcaggagaa gaaaccgatg agtcattcaa cccaggtgaa
     1741 gaggaggaag atgtggcaga ggagtttgac agcaacgcct ctgccagctc ctccagtaat
     1801 gagggtgaca gtgaccggga tgagaagaag cggaaacagc tcaaaaaggc caagatggcc
     1861 aaggaccgca agagccgcaa gaagcctgtg gaggtgaaga agggcaaaga ccccaatgcc
     1921 cccaagaggc ccatgtctgc atacatgctg tggctcaatg ccagccgaga gaagatcaag
     1981 tcagaccatc ctggcatcag catcacggat ctttccaaga aggcaggcga gatctggaag
     2041 ggaatgtcca aagagaagaa agaggagtgg gatcgcaagg ctgaggatgc caggagggac
     2101 tatgaaaaag ccatgaaaga atatgaaggg ggccgaggcg agtcttctaa gagggacaag
     2161 tcaaagaaga agaagaaagt aaaggtaaag atggaaaaga aatccacgcc ctctaggggc
     2221 tcatcatcca agtcgtcctc aaggcagcta agcgagagct tcaagagcaa agagtttgtg
     2281 tctagtgatg agagctcttc gggagagaac aagagcaaaa agaagaggag gaggagcgag
     2341 gactctgaag aagaagaact agccagtact ccccccagct cagaggactc agcgtcagga
     2401 tccgatgagt agaaacggag gaaggttctc tttgcgcttg ccttctcaca ccccccgact
     2461 ccccacccat attttggtac cagtttctcc tcatgaaatg cagtccctgg attctgtgcc
     2521 atctgaacat gctctcctgt tggtgtgtat gtcactaggg cagtggggag acgtcttaac
     2581 tctgctgctt cccaaggatg gctgtttata atttggggag agatagggtg ggaggcaggg
     2641 caatgcagga tccaaatcct catcttactt tcccgacctt aaggatgtag ctgctgcttg
     2701 tcctgttcaa gttgctggag caggggtcat gtgaggccag gcctgtagct cctacctggg
     2761 gcctatttct actttcattt tgtatttctg gtctgtgaaa atgatttaat aaagggaact
     2821 gactttggaa a
//
