LOCUS       NM_003144               9661 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens signal sequence receptor subunit 1 (SSR1), transcript
            variant 1, mRNA.
ACCESSION   NM_003144
VERSION     NM_003144.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Yan,J., Wang,Z.H., Yan,Y., Luo,H.N., Ren,X.Y., Li,N., Zheng,G.X.
            and Hou,J.
  TITLE     RP11-156L14.1 regulates SSR1 expression by competitively binding to
            miR-548ao-3p in hypopharyngeal squamous cell carcinoma
  JOURNAL   Oncol Rep 44 (5), 2080-2092 (2020)
   PUBMED   33000261
  REMARK    GeneRIF: RP11156L14.1 regulates SSR1 expression by competitively
            binding to miR548ao3p in hypopharyngeal squamous cell carcinoma.
REFERENCE   3  (bases 1 to 9661)
  AUTHORS   Liu,X., Salokas,K., Tamene,F., Jiu,Y., Weldatsadik,R.G., Ohman,T.
            and Varjosalo,M.
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive
            mapping of protein interactions and subcellular localizations
  JOURNAL   Nat Commun 9 (1), 1188 (2018)
   PUBMED   29568061
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9661)
  AUTHORS   Mahajan,A., Go,M.J., Zhang,W., Below,J.E., Gaulton,K.J.,
            Ferreira,T., Horikoshi,M., Johnson,A.D., Ng,M.C., Prokopenko,I.,
            Saleheen,D., Wang,X., Zeggini,E., Abecasis,G.R., Adair,L.S.,
            Almgren,P., Atalay,M., Aung,T., Baldassarre,D., Balkau,B., Bao,Y.,
            Barnett,A.H., Barroso,I., Basit,A., Been,L.F., Beilby,J.,
            Bell,G.I., Benediktsson,R., Bergman,R.N., Boehm,B.O.,
            Boerwinkle,E., Bonnycastle,L.L., Burtt,N., Cai,Q., Campbell,H.,
            Carey,J., Cauchi,S., Caulfield,M., Chan,J.C., Chang,L.C.,
            Chang,T.J., Chang,Y.C., Charpentier,G., Chen,C.H., Chen,H.,
            Chen,Y.T., Chia,K.S., Chidambaram,M., Chines,P.S., Cho,N.H.,
            Cho,Y.M., Chuang,L.M., Collins,F.S., Cornelis,M.C., Couper,D.J.,
            Crenshaw,A.T., van Dam,R.M., Danesh,J., Das,D., de Faire,U.,
            Dedoussis,G., Deloukas,P., Dimas,A.S., Dina,C., Doney,A.S.,
            Donnelly,P.J., Dorkhan,M., van Duijn,C., Dupuis,J., Edkins,S.,
            Elliott,P., Emilsson,V., Erbel,R., Eriksson,J.G., Escobedo,J.,
            Esko,T., Eury,E., Florez,J.C., Fontanillas,P., Forouhi,N.G.,
            Forsen,T., Fox,C., Fraser,R.M., Frayling,T.M., Froguel,P.,
            Frossard,P., Gao,Y., Gertow,K., Gieger,C., Gigante,B., Grallert,H.,
            Grant,G.B., Grrop,L.C., Groves,C.J., Grundberg,E., Guiducci,C.,
            Hamsten,A., Han,B.G., Hara,K., Hassanali,N., Hattersley,A.T.,
            Hayward,C., Hedman,A.K., Herder,C., Hofman,A., Holmen,O.L.,
            Hovingh,K., Hreidarsson,A.B., Hu,C., Hu,F.B., Hui,J.,
            Humphries,S.E., Hunt,S.E., Hunter,D.J., Hveem,K., Hydrie,Z.I.,
            Ikegami,H., Illig,T., Ingelsson,E., Islam,M., Isomaa,B.,
            Jackson,A.U., Jafar,T., James,A., Jia,W., Jockel,K.H., Jonsson,A.,
            Jowett,J.B., Kadowaki,T., Kang,H.M., Kanoni,S., Kao,W.H.,
            Kathiresan,S., Kato,N., Katulanda,P., Keinanen-Kiukaanniemi,K.M.,
            Kelly,A.M., Khan,H., Khaw,K.T., Khor,C.C., Kim,H.L., Kim,S.,
            Kim,Y.J., Kinnunen,L., Klopp,N., Kong,A., Korpi-Hyovalti,E.,
            Kowlessur,S., Kraft,P., Kravic,J., Kristensen,M.M., Krithika,S.,
            Kumar,A., Kumate,J., Kuusisto,J., Kwak,S.H., Laakso,M., Lagou,V.,
            Lakka,T.A., Langenberg,C., Langford,C., Lawrence,R., Leander,K.,
            Lee,J.M., Lee,N.R., Li,M., Li,X., Li,Y., Liang,J., Liju,S.,
            Lim,W.Y., Lind,L., Lindgren,C.M., Lindholm,E., Liu,C.T., Liu,J.J.,
            Lobbens,S., Long,J., Loos,R.J., Lu,W., Luan,J., Lyssenko,V.,
            Ma,R.C., Maeda,S., Magi,R., Mannisto,S., Matthews,D.R., Meigs,J.B.,
            Melander,O., Metspalu,A., Meyer,J., Mirza,G., Mihailov,E.,
            Moebus,S., Mohan,V., Mohlke,K.L., Morris,A.D., Muhleisen,T.W.,
            Muller-Nurasyid,M., Musk,B., Nakamura,J., Nakashima,E., Navarro,P.,
            Ng,P.K., Nica,A.C., Nilsson,P.M., Njolstad,I., Nothen,M.M.,
            Ohnaka,K., Ong,T.H., Owen,K.R., Palmer,C.N., Pankow,J.S.,
            Park,K.S., Parkin,M., Pechlivanis,S., Pedersen,N.L., Peltonen,L.,
            Perry,J.R., Peters,A., Pinidiyapathirage,J.M., Platou,C.G.,
            Potter,S., Price,J.F., Qi,L., Radha,V., Rallidis,L., Rasheed,A.,
            Rathman,W., Rauramaa,R., Raychaudhuri,S., Rayner,N.W., Rees,S.D.,
            Rehnberg,E., Ripatti,S., Robertson,N., Roden,M., Rossin,E.J.,
            Rudan,I., Rybin,D., Saaristo,T.E., Salomaa,V., Saltevo,J.,
            Samuel,M., Sanghera,D.K., Saramies,J., Scott,J., Scott,L.J.,
            Scott,R.A., Segre,A.V., Sehmi,J., Sennblad,B., Shah,N., Shah,S.,
            Shera,A.S., Shu,X.O., Shuldiner,A.R., Sigurdsson,G., Sijbrands,E.,
            Silveira,A., Sim,X., Sivapalaratnam,S., Small,K.S., So,W.Y.,
            Stancakova,A., Stefansson,K., Steinbach,G., Steinthorsdottir,V.,
            Stirrups,K., Strawbridge,R.J., Stringham,H.M., Sun,Q., Suo,C.,
            Syvanen,A.C., Takayanagi,R., Takeuchi,F., Tay,W.T., Teslovich,T.M.,
            Thorand,B., Thorleifsson,G., Thorsteinsdottir,U., Tikkanen,E.,
            Trakalo,J., Tremoli,E., Trip,M.D., Tsai,F.J., Tuomi,T.,
            Tuomilehto,J., Uitterlinden,A.G., Valladares-Salgado,A.,
            Vedantam,S., Veglia,F., Voight,B.F., Wang,C., Wareham,N.J.,
            Wennauer,R., Wickremasinghe,A.R., Wilsgaard,T., Wilson,J.F.,
            Wiltshire,S., Winckler,W., Wong,T.Y., Wood,A.R., Wu,J.Y., Wu,Y.,
            Yamamoto,K., Yamauchi,T., Yang,M., Yengo,L., Yokota,M., Young,R.,
            Zabaneh,D., Zhang,F., Zhang,R., Zheng,W., Zimmet,P.Z.,
            Altshuler,D., Bowden,D.W., Cho,Y.S., Cox,N.J., Cruz,M., Hanis,C.L.,
            Kooner,J., Lee,J.Y., Seielstad,M., Teo,Y.Y., Boehnke,M.,
            Parra,E.J., Chambers,J.C., Tai,E.S., McCarthy,M.I. and Morris,A.P.
  CONSRTM   DIAbetes Genetics Replication And Meta-analysis (DIAGRAM)
            Consortium; Asian Genetic Epidemiology Network Type 2 Diabetes
            (AGEN-T2D) Consortium; South Asian Type 2 Diabetes (SAT2D)
            Consortium; Mexican American Type 2 Diabetes (MAT2D) Consortium;
            Type 2 Diabetes Genetic Exploration by Nex-generation sequencing in
            muylti-Ethnic Samples (T2D-GENES) Consortium
  TITLE     Genome-wide trans-ancestry meta-analysis provides insight into the
            genetic architecture of type 2 diabetes susceptibility
  JOURNAL   Nat Genet 46 (3), 234-244 (2014)
   PUBMED   24509480
REFERENCE   5  (bases 1 to 9661)
  AUTHORS   Lakkaraju,A.K., Abrami,L., Lemmin,T., Blaskovic,S., Kunz,B.,
            Kihara,A., Dal Peraro,M. and van der Goot,F.G.
  TITLE     Palmitoylated calnexin is a key component of the
            ribosome-translocon complex
  JOURNAL   EMBO J 31 (7), 1823-1835 (2012)
   PUBMED   22314232
REFERENCE   6  (bases 1 to 9661)
  AUTHORS   Hu,T., Guan,T. and Gerace,L.
  TITLE     Molecular and functional characterization of the p62 complex, an
            assembly of nuclear pore complex glycoproteins
  JOURNAL   J Cell Biol 134 (3), 589-601 (1996)
   PUBMED   8707840
REFERENCE   7  (bases 1 to 9661)
  AUTHORS   Hartmann,E. and Prehn,S.
  TITLE     The N-terminal region of the alpha-subunit of the TRAP complex has
            a conserved cluster of negative charges
  JOURNAL   FEBS Lett 349 (3), 324-326 (1994)
   PUBMED   8050590
REFERENCE   8  (bases 1 to 9661)
  AUTHORS   Hartmann,E., Gorlich,D., Kostka,S., Otto,A., Kraft,R., Knespel,S.,
            Burger,E., Rapoport,T.A. and Prehn,S.
  TITLE     A tetrameric complex of membrane proteins in the endoplasmic
            reticulum
  JOURNAL   Eur J Biochem 214 (2), 375-381 (1993)
   PUBMED   7916687
REFERENCE   9  (bases 1 to 9661)
  AUTHORS   Wei,M.L. and Cresswell,P.
  TITLE     HLA-A2 molecules in an antigen-processing mutant cell contain
            signal sequence-derived peptides
  JOURNAL   Nature 356 (6368), 443-446 (1992)
   PUBMED   1557127
REFERENCE   10 (bases 1 to 9661)
  AUTHORS   Vogel,F., Hartmann,E., Gorlich,D. and Rapoport,T.A.
  TITLE     Segregation of the signal sequence receptor protein in the rough
            endoplasmic reticulum membrane
  JOURNAL   Eur J Cell Biol 53 (2), 197-202 (1990)
   PUBMED   1964414
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC007710.2, AL139095.15 and
            BM685131.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003144.4.
            
            Summary: The signal sequence receptor (SSR) is a glycosylated
            endoplasmic reticulum (ER) membrane receptor associated with
            protein translocation across the ER membrane. The SSR consists of 2
            subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD
            glycoprotein. This gene generates several mRNA species as a result
            of complex alternative polyadenylation. This gene is unusual in
            that it utilizes arrays of polyA signal sequences that are mostly
            non-canonical. Multiple transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, May
            2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.54577.1,
                                           SRR1660803.67255.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000244763.9/ ENSP00000244763.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1244              BC007710.2         9-1252
            1245-9021           AL139095.15        42448-50224         c
            9022-9661           BM685131.1         18-657              c
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p24.3"
     gene            1..9661
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /note="signal sequence receptor subunit 1"
                     /db_xref="GeneID:6745"
                     /db_xref="HGNC:HGNC:11323"
                     /db_xref="MIM:600868"
     exon            1..158
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..940
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; TRAP alpha; translocon-associated protein
                     subunit alpha; translocon-associated protein alpha
                     subunit; SSR alpha subunit; SSR-alpha; signal sequence
                     receptor subunit alpha; signal sequence receptor, alpha"
                     /codon_start=1
                     /product="translocon-associated protein subunit alpha
                     isoform 1 precursor"
                     /protein_id="NP_003135.2"
                     /db_xref="CCDS:CCDS4499.1"
                     /db_xref="GeneID:6745"
                     /db_xref="HGNC:HGNC:11323"
                     /db_xref="MIM:600868"
                     /translation="MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVED
                     SIIEDEDDEAEVEEDEPTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVK
                     FLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIP
                     AEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLG
                     LLVIVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKR
                     AQKRSVGSDE"
     exon            159..271
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            272..359
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            360..622
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            623..699
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            700..778
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            779..872
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            873..9661
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3205..3210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /note="hexamer: AATAAT"
     polyA_site      3234
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /note="major polyA site"
     regulatory      9640..9645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
                     /note="hexamer: ATTAAA"
     polyA_site      9661
                     /gene="SSR1"
                     /gene_synonym="TRAPA"
ORIGIN      
        1 ggatgaagag taacgccatt accgccggag ccgccgagag ccttagccga cggaaactgg
       61 acactggacc ggcagcgcca tgagactcct cccccgcttg ctgctgcttc tcttactcgt
      121 gttccctgcc actgtcttgt tccgaggcgg ccccagaggc ttgttagcag tggcacaaga
      181 tcttacagag gatgaagaaa cagtagaaga ttccataatt gaggatgaag atgatgaagc
      241 cgaggtagaa gaagatgaac ccacagattt ggtagaagat aaagaggaag aagatgtgtc
      301 tggtgaacct gaagcttcac cgagtgcaga tacaactata ctgtttgtaa aaggagaaga
      361 ttttccagca aataacattg tgaagttcct ggtaggcttt accaacaagg gtacagaaga
      421 ttttattgtt gaatccttag atgcctcatt ccgttatcct caggactacc agttttatat
      481 ccagaatttc acagctcttc ctctgaacac tgtagtgcca ccccagagac aggcaacttt
      541 tgagtactct ttcattcctg cagagcccat gggcggacga ccatttggtt tggtcatcaa
      601 tctgaactac aaagatttga acggcaatgt attccaagat gcagtcttca atcaaacagt
      661 tacagttatt gaaagagagg atgggttaga tggagaaaca atctttatgt atatgttcct
      721 tgctggtctt gggcttctgg ttattgttgg ccttcatcaa ctcctagaat ctagaaagcg
      781 taagagaccc atacagaaag tagaaatggg tacatcaagt cagaatgatg ttgacatgag
      841 ttggattcct caggaaacat tgaatcaaat caataaagct tcaccaagaa ggttgcccag
      901 gaaacgggca cagaagagat cagtgggatc tgatgagtaa atgttccttt gtgcaacaat
      961 tcggtcttta cttaacctgc cctaatattt ttcggcctga tgggaattag tgcagagaag
     1021 ccatgtcacc atagaaggca actcctactt gtgtgtggac tgagcaatca gagtctgtgg
     1081 cgataatatt gctgaaaatg cactgcattc atttttctaa agtaacaaat ttggtttttt
     1141 tttaaaccat taaaatctat gtgtgtgcgt gtgtatgtat gtgagcagtt ggtcttacca
     1201 gaatcattgt tgaactacct gaaacaagtc tttagaatac taaatataat gctgttgtct
     1261 cttccttttt gacattttct gattttttcc cccaaaactc agttaatatt tacccactat
     1321 gattattgat gtcctgcctt gaacagtttt aaagaaaaca atttttggaa tagctcaaat
     1381 ttcaattgat ggcacaaatc agcattttgt tgttgttact gtattacaat tagtattcta
     1441 aaggcagaag cagaagtagc tgctttttag caatagaatt gtttcagtat tttgctgctg
     1501 tttaatgcgc atcttcagaa aacttcccag tggcttcaag gaatttgggg atctctctgg
     1561 caacaaattg tgaaacatga aatttctgct gactttaata tatgaaacct aatcctaccc
     1621 ccttttttaa caaaaagaaa ctagtacatt tgtgaaaatt gtgttgtgtt gtccattgtt
     1681 gctctagttc tgacccagag gtagctctgg agtgatttta gacctactca ctcagttgtg
     1741 tgtaggtttt tttgttttgt tttgagagag aatttttctc tccttaatag aagcatcctt
     1801 tttaaagaga agttgccttg gtccacacac taagcagaaa accaagttat caggacagag
     1861 atatttccca gttactccta atcaatgaag aaagtgagtt ggatattttt aaagcagtta
     1921 actaattttt tcttacctaa tcttttggga gttttgcttg ttgatataac ctttttagtt
     1981 aacctgaaag attccaaaaa ttgttcttaa gtgcttgaga ctggaaccaa aattaaattg
     2041 tacttcataa aatcctctta tagagttact cttgccctag attgtaaatt aagtttggca
     2101 ttattgtcag actggatgga gggtgaagta aaatagtatg aacaattaag aggctctccc
     2161 cctcttgtct ttaagccata ttctcctaca tgtattttat aagaaaatgt taagtcaaat
     2221 tttagtggct ctttaattcc tgacctcttc attctccttt tcagtataac ctcccctatg
     2281 ctcatgccca cacagacaaa aaaacaaaac gaaatacaca cagaaaaaag tctttccaaa
     2341 ctgtttaagt atttaaacat ctgagccaaa gcagatagaa gttattgtat aattgttaat
     2401 cactttgcaa ataggggcta tcaaattacc tatattggca ttgctggatt ataaactcta
     2461 tatctgtaat ataaagtgtt tgagttttta attgggctgt tatgatcagt agttgatttt
     2521 gagaaagctc tatgagctct aagtaactgc atggtttttt gtttaatgta atataggaga
     2581 cccttcacat tcccaaggaa tatattccaa aacatttttg tgaatatcta agtttgtgaa
     2641 actactaggg catgatacag taaggtgtaa ttacagaatt tacgaaatgt aaatggcctc
     2701 tacagagttt tatggaatac ctggtactaa cgtaggcagc tgcaaaacca cactgagtta
     2761 cagctgtcag ccctcctcat tcctaaataa cttgccttac atatcagccc tcccacttct
     2821 gaagttcaaa ttagtgcctc ggaaatgtag aatttattat ttgtcatttt ttttttttta
     2881 gcatagattg agaacagttg aactcttaaa tcctcagatg ccaggggtct gctctagcat
     2941 cagtaagtat ttagcagaaa ctaactccgt aatgaatgga attcaattcc acacatggtt
     3001 tgttcaagca cacttaataa gtagcctatt ttttaaatgt ctttttaaaa tgtaaatatt
     3061 tggatgaagt ttttctttgt tttgatatat tcatttgcta caccaactat gttttcagaa
     3121 ttcatctttt gaacaacttg gtttcagaat atgtaaaatg actttaagga tcttgtgtat
     3181 caaacctatc cccggatgtg tgagaataat gtgttcataa agcatggatc tcgctttggt
     3241 tgtatagctt cctcatttac ttcatggtct tacatagctg gtgactttca gggctaatct
     3301 gccctctaaa gcattgtccc aggagaggaa aaggaaatgg gacctcagaa gtagaagcct
     3361 cagggaagga gtaaagtaga aatcagaaga aaagaagctt cacttgatag taataaggtt
     3421 tttaacttca agtaccttca gaaaatgtga ttttgataag aggaaagggc aaatttagac
     3481 cttaaaaaat atggaagaac tactgcctta aaagtgcatt tgtggcacat cagcctagaa
     3541 ctgtatcatg gctgtgctgg ggagaagtaa atggtggtaa tgtaacattg ccacctttac
     3601 ttaatgatgt gttattttcg aggtacagta gatcaatata gtaataggcg agcctcatat
     3661 atagcattca tcttgtacaa tgatatccat acccttgata tgaaggaaaa ttgacttggt
     3721 ttgtgcattt gaatactgaa ataatttttt aaaactcagt gacacatacc atctcttgcc
     3781 aagactagac cctgtatttt agttcctaaa ttagatgttt aaatttaaaa acattttcag
     3841 atgtacttaa gtacttccat agtacttttt tttttttttt tttttttgcc ccctgagacg
     3901 gagtctctgt gtcacccagg ctggagtgca gtggtgcgat ctcggctcac tgcaacttct
     3961 gcctcttggg ttcaagcagt tctccctgtc tcagcctcct gagtagcttg gattacaggc
     4021 gcccgtcacc gcacctgcct aatttttgca tttttagtag agacggggtt tcgtcatttg
     4081 gccaggctgg tcttgaactc ctgaccacag gtgatacgcc tgccttggcc tcccaaagtg
     4141 tgctgggatt acaggcgtga gccactgtgc ccggcccact gttcactttt tgaatggcat
     4201 cattttatag ctgtagaact aaaatcaatg tttgccccaa ttttcttaag taaaactcta
     4261 ctttgagctc ttacctccaa cttagtaaaa agcagcttca cacacaaaca agattcttac
     4321 tggtggaatg ttaggtttcg ttgttaagtt aatctgtcta taagctcatc cttagaggat
     4381 atttgaggag gaagaacacc ttgcagctga cttgcaaaca tctaaataat ttatttcggg
     4441 tgcttatgaa tgttactaat ggattttgta tgaattttta tcccttttca tttatacaaa
     4501 aacctgggct tttatgttaa ttatatcatc tgaggttcta aggttttttt tttagatttt
     4561 gaaatttagg gataatagct cttaggtttg ggtaccactt tgctgcagtt taagaaaggg
     4621 ggaagggaac tcatttatta aacatcaatc acgtgctgtg ttctgtttgt tttctagtca
     4681 tcatatcaca cacctttacg acagctcact gaaggaaggt gatactgttc ccattttgta
     4741 gatggaatag acaaaacctg aatttaagta gcttgctcaa ggttccatat tgaatatgga
     4801 aagttcaaat catctcagta atgaatatac catatatact tgctgtattg tatctatgat
     4861 aattcagtta cccacaatac ccttttaaat ttctgttaat gacatacctt taaatgtctc
     4921 cttgatgaac agaatcatgg tctttaaaaa cattttcatg ggttgattgc attttcaagc
     4981 tctaaaggat tgaaagataa atcttcacgt taaaggtaag agtgaagtat ctgctcttgg
     5041 gttacagaac cagatagtac tagaactaag attacagggt aaagctgctt ttatcttttt
     5101 tctttttctt tttctttttt tttttgacat ggggtctcac tgtattgccc aggctggaat
     5161 gcagtggcat gatctcagct cacggcagcc tctgcctctt gggctcaagc gattctcctg
     5221 cttcagcttt ccaagtattt gggaccacag gcgcacacca caggcctggc taatgttttt
     5281 gttttgtttt tggtagagac ggggtttcac catgttgcca ggctggtctc gaactcctga
     5341 gctcaagtga ttcacccacc ttggcctcac aaagtgtcag gcttacaggc gtgagccact
     5401 gcgcccggct cacagggtaa ggcttctgtc tggtgtgttg tattacggat tttgcttaat
     5461 aggcacagtg aggcattaaa aagaaaattc agtatgcctg tagaaaggat aatccttgtt
     5521 taaagtctcc aaattgcagt caaagatgtt ttgactgtgc ctttttttgt tcccctgctg
     5581 tcccttatgt agacttctgt cagtacccat ggcagcctgt catcttgttg acatctcctt
     5641 ctggactgtg agctctgtat ctggcttgtt tttcatcccc agcttctagt tcacaattag
     5701 gtagaaccct attactcttt gaagaaggaa caagaaaatg tgggccagtt ttcatttgcc
     5761 attcttccat gtgagttagt atggttcgta agtattcctg gtgatacgct agtattggca
     5821 attctgtgag gttgaacaaa ggggtggtat ggtgtgctag cgtgggaatt aggagacctc
     5881 tgggtcttga cagtgccctg gccactaagc aaaggcagtt catccttgga gtctcaatgt
     5941 gcttttttgt taattgagat atgcttgaag tatcagccct aaatagtctg attctgtgac
     6001 ctacaaaccc ttacttaatt cagtgttact ataaatgatt cttcccttaa acctactttt
     6061 tacttagcaa aagagaaaaa aaaaaaaagg aaacgctcat gtcaggctgc ctgggtttga
     6121 accccagatc ccctcttagt tggaggcaag ttgtatgatg cttcagcttt tggttcctgc
     6181 ctcctaaggt tgttaggagt tactgtgtgt agctgcttag aacattgcct gggtctcagt
     6241 aagccgctta agtgactgct ctcattttcg ctgtaaagca ccatactgta ataacatccc
     6301 atgaagcatg gggcggggaa gagtatatgg taccttatgg actttgatgt ggtggggtag
     6361 taggtaaatt ctgaatatgt aagctacata gtattctttt tgtaactaaa ggataaaagt
     6421 tttaagatgg gcatgtaata tggctagcac tggattttaa tgatgagcca gagtaataag
     6481 gctggcaagg ggagtttttg ttttgtaata aatctcttac acctgcacat ccagttcttt
     6541 ttaaaaacac gtttgaagag gctccatatt tctcagtgaa gctgttgggg ttcatgttat
     6601 ttttgaaaat catcttgcaa tttatttcag catcagactg aaccacccaa agaaaattag
     6661 tctatttctg aacagttttt tcagaaacca tattggtctg atcacccaac atttatagaa
     6721 ataggcgctt aaggcctaga ggcatttcag aaaggagaat gagaaatact gtggtcaaga
     6781 gtagtgttca gatggagaac atcaagcatc tgctctgccc ttccacacac atcctcattt
     6841 catcttcaca actgccctag gtaaccttgt tatctgtaat gaaacagcct caaggagcac
     6901 agaggcactc actcctcacg tttggtctgt tgccatttcc ggcttggttg gaatagggta
     6961 ggagcctttt ggcagggagc acattctcag taatgcagag tgcactcacc tgggttctag
     7021 cttcaacact taggatttgc ttgatatttt gtattctctg aggcactgcc tgtatttgtt
     7081 tctgctatca cagtccagag tcaagcttca tttataaaga ctgggcaggg catggtggct
     7141 cactcctgta attccagcac tttgggaggc tgaggtgggt ggatcacttt aggtcaggag
     7201 ttcaagacca gcctggccaa catggtgaaa ccccatctct actaaaaaat acaaaaaggc
     7261 cgggcacggt ggctcacacc tgtaatccca acactttggg aggccaaggt gggcagaaca
     7321 cctgaggtca ggagttcaag accatcctgg cgaacatagt gaaacctcgc ctctactaaa
     7381 aatacaaaaa ttagccaggt gtggtggtgc atgcctgtaa tcccagctac ttgggaggct
     7441 gaggcaggag aattgcttaa acctgggagg tggagattgt ggtgagctga gatcgtgcca
     7501 ctgcactcca gcctgggaga tagaacgaga ctccatctca aaaaagaaca aaaacaatta
     7561 gccgagcgag gtggtgcacg cctgtaatcc cagctactca tgaggctgag gcaggagaat
     7621 cacttgaacc caggaggagg aggttgtagt gagtcaaggt tgcaccactg cactccagcc
     7681 tgggtgacag agtgagactg tctcaaaaaa taagtacata aataataagt aaaagctact
     7741 aacaattaaa aaataaataa ataaagacaa gactgtctgg aaaatggctc tcctaaaagg
     7801 accagttgcc atcatccaca gtggaagatt caaagcagtt ggtccttggt acgtatgaga
     7861 agcggatttc attcccttga attctacaga gcagtttatt agagtgaatg cattttaagg
     7921 ccttgcattt gatatgtcat ccagttcata atcaagttgc ctttttctgg ctaaaacata
     7981 atgattatgt atttttctca tttggtccta caagctgctg gccctttgtc cctccactgt
     8041 gggaatcaga tctagaggag gctgagcctg cagacacagc agtggccaaa aggtcactct
     8101 aagtgttttg tcttgactcc ttacttgaag tccacccagc tagcacacat ctggtttata
     8161 ctgaagcccc ctgcctagaa atactcattt caggaaccac cagtaagcat ctgtgaccac
     8221 acaggctttt tgactgatgg cttcccggat ctggtttcaa gggataaccc cgtctgtgtg
     8281 catctatggt cttctctcta cagcgaggac tttgcagtgc tgcttgtggt ccacacaagg
     8341 ggctcagagc tgagtctgaa ctgcttcatg gtcaccagct cctgtccctt ccagtcttga
     8401 gaggcttttt tctccagatg gaacctttcc ttcccgccgt tttctcggtc tctggctgtt
     8461 tttctcttgt gcccgtctaa ttggacacct cctggcttcc atctctgtgg ttctcctgcc
     8521 tcacttcctg ttctgttgtt tttccgtttt gtcaaaatat ctcctatgtt cttggcttcc
     8581 ttttcgtcgc caggttttca gctttccttt agctcttctt ctaatatggc ttctgcccac
     8641 aaaagcctgc tctgtcagga tctcatggtt ctccacttgc cagaaccttc ttcagcctca
     8701 gttcctcggc ctcaacttgt acgtttaacc cattgaccac caccccccaa attcaccttc
     8761 atttctttga ccctgctcct cactcctttt ctgttgagga atctgttgac taactccagg
     8821 ctcactcagg ctcaccgtcc tgctctctgc accagccttt ccagagcgtg ccagttctca
     8881 tggcttcatc tgttaactgt tgatcacttc agtcctgatt tttagaccta aatggtttcc
     8941 ttaacgccat tctaactgcc tgtgactcat tttcacttac agtgtttatt gtaacgccaa
     9001 accaacaaat cacaggtgct tgcttctctc cataaatctc cccagtctaa ctttttgtca
     9061 ttcaacatga ctcgtttatc caacctgaaa tcgcatatag ccccaagtat ggtgttttgt
     9121 acacaggtat ttaataagtg acttccagtt ttggctctgc tatgaataaa aagagatttc
     9181 agttctcttc actttgaaat ctaacaactc agagaacatt gaagaaattg gaatttagtt
     9241 gggatgaaat acttgtggtt taaaatattt ctgttcatat tttctaattt gttgccggag
     9301 gtcttgggtt ttctatttga gtgcttgcaa actcaatgtg atttctgtca gcatatctta
     9361 ggtttgtttg ttatgaaact tatgcagtgt gaggttctat ctgaaaatgt tatttagcta
     9421 tcttctggga ctatttaatg aaagtggggt catgaatcct taaaattctt gtgcagcttt
     9481 gagaaacatt tctgttattt gggtatcagt ttgtaagtgt ggtaaagcca agatggaaac
     9541 gagcactttg ctttcttggt tgttgttact ggtctaacct cctgcttgaa ctagtctgct
     9601 gtcctgtcaa atgcatcttt ttatttacat gtcccttaaa ttaaagctga tcatgaaagt
     9661 a
//
