LOCUS       NM_005086               4533 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens sarcospan (SSPN), transcript variant 1, mRNA.
ACCESSION   NM_005086
VERSION     NM_005086.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Panzer,A.A., Regmi,S.D., Cormier,D., Danzo,M.T., Chen,I.D.,
            Winston,J.B., Hutchinson,A.K., Salm,D., Schulkey,C.E.,
            Cochran,R.S., Wilson,D.B. and Jay,P.Y.
  TITLE     Nkx2-5 and Sarcospan genetically interact in the development of the
            muscular ventricular septum of the heart
  JOURNAL   Sci Rep 7, 46438 (2017)
   PUBMED   28406175
  REMARK    GeneRIF: Data indicate that Sspn is an Nkx2-5 modifier gene.
            Complete loss of Sspn function does not cause heart defects but
            does increase the incidence of muscular ventricular septal defects
            (VSDs) in combination with an Nkx2-5 mutation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Gibbs,E.M., Marshall,J.L., Ma,E., Nguyen,T.M., Hong,G., Lam,J.S.,
            Spencer,M.J. and Crosbie-Watson,R.H.
  TITLE     High levels of sarcospan are well tolerated and act as a
            sarcolemmal stabilizer to address skeletal muscle and pulmonary
            dysfunction in DMD
  JOURNAL   Hum Mol Genet 25 (24), 5395-5406 (2016)
   PUBMED   27798107
  REMARK    GeneRIF: When human SSPN is expressed at three-fold levels in mdx
            mice, this increase in adhesion complex abundance improves muscle
            membrane stability, preventing many of the histopathological
            changes associated with Duchenne muscular dystrophy
REFERENCE   3  (bases 1 to 4533)
  AUTHORS   Chew,E.G.Y., Tan,J.H.J., Bahta,A.W., Ho,B.S., Liu,X., Lim,T.C.,
            Sia,Y.Y., Bigliardi,P.L., Heilmann,S., Wan,A.C.A., Nothen,M.M.,
            Philpott,M.P. and Hillmer,A.M.
  TITLE     Differential Expression between Human Dermal Papilla Cells from
            Balding and Non-Balding Scalps Reveals New Candidate Genes for
            Androgenetic Alopecia
  JOURNAL   J Invest Dermatol 136 (8), 1559-1567 (2016)
   PUBMED   27060448
  REMARK    GeneRIF: Our data suggest TWIST1 and SSPN to be the functionally
            relevant androgenetic alopecia genes at the 7p21.1 and 12p12.1 risk
            loci, respectively.
REFERENCE   4  (bases 1 to 4533)
  AUTHORS   Heid,I.M., Jackson,A.U., Randall,J.C., Winkler,T.W., Qi,L.,
            Steinthorsdottir,V., Thorleifsson,G., Zillikens,M.C.,
            Speliotes,E.K., Magi,R., Workalemahu,T., White,C.C.,
            Bouatia-Naji,N., Harris,T.B., Berndt,S.I., Ingelsson,E.,
            Willer,C.J., Weedon,M.N., Luan,J., Vedantam,S., Esko,T.,
            Kilpelainen,T.O., Kutalik,Z., Li,S., Monda,K.L., Dixon,A.L.,
            Holmes,C.C., Kaplan,L.M., Liang,L., Min,J.L., Moffatt,M.F.,
            Molony,C., Nicholson,G., Schadt,E.E., Zondervan,K.T., Feitosa,M.F.,
            Ferreira,T., Lango Allen,H., Weyant,R.J., Wheeler,E., Wood,A.R.,
            Estrada,K., Goddard,M.E., Lettre,G., Mangino,M., Nyholt,D.R.,
            Purcell,S., Smith,A.V., Visscher,P.M., Yang,J., McCarroll,S.A.,
            Nemesh,J., Voight,B.F., Absher,D., Amin,N., Aspelund,T., Coin,L.,
            Glazer,N.L., Hayward,C., Heard-Costa,N.L., Hottenga,J.J.,
            Johansson,A., Johnson,T., Kaakinen,M., Kapur,K., Ketkar,S.,
            Knowles,J.W., Kraft,P., Kraja,A.T., Lamina,C., Leitzmann,M.F.,
            McKnight,B., Morris,A.P., Ong,K.K., Perry,J.R., Peters,M.J.,
            Polasek,O., Prokopenko,I., Rayner,N.W., Ripatti,S., Rivadeneira,F.,
            Robertson,N.R., Sanna,S., Sovio,U., Surakka,I., Teumer,A., van
            Wingerden,S., Vitart,V., Zhao,J.H., Cavalcanti-Proenca,C.,
            Chines,P.S., Fisher,E., Kulzer,J.R., Lecoeur,C., Narisu,N.,
            Sandholt,C., Scott,L.J., Silander,K., Stark,K., Tammesoo,M.L.,
            Teslovich,T.M., Timpson,N.J., Watanabe,R.M., Welch,R.,
            Chasman,D.I., Cooper,M.N., Jansson,J.O., Kettunen,J.,
            Lawrence,R.W., Pellikka,N., Perola,M., Vandenput,L., Alavere,H.,
            Almgren,P., Atwood,L.D., Bennett,A.J., Biffar,R., Bonnycastle,L.L.,
            Bornstein,S.R., Buchanan,T.A., Campbell,H., Day,I.N., Dei,M.,
            Dorr,M., Elliott,P., Erdos,M.R., Eriksson,J.G., Freimer,N.B.,
            Fu,M., Gaget,S., Geus,E.J., Gjesing,A.P., Grallert,H., Grassler,J.,
            Groves,C.J., Guiducci,C., Hartikainen,A.L., Hassanali,N.,
            Havulinna,A.S., Herzig,K.H., Hicks,A.A., Hui,J., Igl,W.,
            Jousilahti,P., Jula,A., Kajantie,E., Kinnunen,L., Kolcic,I.,
            Koskinen,S., Kovacs,P., Kroemer,H.K., Krzelj,V., Kuusisto,J.,
            Kvaloy,K., Laitinen,J., Lantieri,O., Lathrop,G.M., Lokki,M.L.,
            Luben,R.N., Ludwig,B., McArdle,W.L., McCarthy,A., Morken,M.A.,
            Nelis,M., Neville,M.J., Pare,G., Parker,A.N., Peden,J.F.,
            Pichler,I., Pietilainen,K.H., Platou,C.G., Pouta,A.,
            Ridderstrale,M., Samani,N.J., Saramies,J., Sinisalo,J., Smit,J.H.,
            Strawbridge,R.J., Stringham,H.M., Swift,A.J., Teder-Laving,M.,
            Thomson,B., Usala,G., van Meurs,J.B., van Ommen,G.J., Vatin,V.,
            Volpato,C.B., Wallaschofski,H., Walters,G.B., Widen,E., Wild,S.H.,
            Willemsen,G., Witte,D.R., Zgaga,L., Zitting,P., Beilby,J.P.,
            James,A.L., Kahonen,M., Lehtimaki,T., Nieminen,M.S., Ohlsson,C.,
            Palmer,L.J., Raitakari,O., Ridker,P.M., Stumvoll,M., Tonjes,A.,
            Viikari,J., Balkau,B., Ben-Shlomo,Y., Bergman,R.N., Boeing,H.,
            Smith,G.D., Ebrahim,S., Froguel,P., Hansen,T., Hengstenberg,C.,
            Hveem,K., Isomaa,B., Jorgensen,T., Karpe,F., Khaw,K.T., Laakso,M.,
            Lawlor,D.A., Marre,M., Meitinger,T., Metspalu,A., Midthjell,K.,
            Pedersen,O., Salomaa,V., Schwarz,P.E., Tuomi,T., Tuomilehto,J.,
            Valle,T.T., Wareham,N.J., Arnold,A.M., Beckmann,J.S., Bergmann,S.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Collins,F.S.,
            Eiriksdottir,G., Gudnason,V., Gyllensten,U., Hamsten,A.,
            Hattersley,A.T., Hofman,A., Hu,F.B., Illig,T., Iribarren,C.,
            Jarvelin,M.R., Kao,W.H., Kaprio,J., Launer,L.J., Munroe,P.B.,
            Oostra,B., Penninx,B.W., Pramstaller,P.P., Psaty,B.M.,
            Quertermous,T., Rissanen,A., Rudan,I., Shuldiner,A.R., Soranzo,N.,
            Spector,T.D., Syvanen,A.C., Uda,M., Uitterlinden,A., Volzke,H.,
            Vollenweider,P., Wilson,J.F., Witteman,J.C., Wright,A.F.,
            Abecasis,G.R., Boehnke,M., Borecki,I.B., Deloukas,P.,
            Frayling,T.M., Groop,L.C., Haritunians,T., Hunter,D.J.,
            Kaplan,R.C., North,K.E., O'Connell,J.R., Peltonen,L.,
            Schlessinger,D., Strachan,D.P., Hirschhorn,J.N., Assimes,T.L.,
            Wichmann,H.E., Thorsteinsdottir,U., van Duijn,C.M., Stefansson,K.,
            Cupples,L.A., Loos,R.J., Barroso,I., McCarthy,M.I., Fox,C.S.,
            Mohlke,K.L. and Lindgren,C.M.
  CONSRTM   MAGIC
  TITLE     Meta-analysis identifies 13 new loci associated with waist-hip
            ratio and reveals sexual dimorphism in the genetic basis of fat
            distribution
  JOURNAL   Nat Genet 42 (11), 949-960 (2010)
   PUBMED   20935629
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Erratum:[Nat Genet. 2011 Nov;43(11):1164]
REFERENCE   5  (bases 1 to 4533)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   6  (bases 1 to 4533)
  AUTHORS   Yoshida,M., Hama,H., Ishikawa-Sakurai,M., Imamura,M., Mizuno,Y.,
            Araishi,K., Wakabayashi-Takai,E., Noguchi,S., Sasaoka,T. and
            Ozawa,E.
  TITLE     Biochemical evidence for association of dystrobrevin with the
            sarcoglycan-sarcospan complex as a basis for understanding
            sarcoglycanopathy
  JOURNAL   Hum Mol Genet 9 (7), 1033-1040 (2000)
   PUBMED   10767327
REFERENCE   7  (bases 1 to 4533)
  AUTHORS   Noguchi,S., Wakabayashi,E., Imamura,M., Yoshida,M. and Ozawa,E.
  TITLE     Formation of sarcoglycan complex with differentiation in cultured
            myocytes
  JOURNAL   Eur J Biochem 267 (3), 640-648 (2000)
   PUBMED   10651799
REFERENCE   8  (bases 1 to 4533)
  AUTHORS   Crosbie,R.H., Heighway,J., Venzke,D.P., Lee,J.C. and Campbell,K.P.
  TITLE     Sarcospan, the 25-kDa transmembrane component of the
            dystrophin-glycoprotein complex
  JOURNAL   J Biol Chem 272 (50), 31221-31224 (1997)
   PUBMED   9395445
REFERENCE   9  (bases 1 to 4533)
  AUTHORS   Heighway,J., Betticher,D.C., Hoban,P.R., Altermatt,H.J. and
            Cowen,R.
  TITLE     Coamplification in tumors of KRAS2, type 2 inositol 1,4,5
            triphosphate receptor gene, and a novel human gene, KRAG
  JOURNAL   Genomics 35 (1), 207-214 (1996)
   PUBMED   8661122
REFERENCE   10 (bases 1 to 4533)
  AUTHORS   Scott,A.F., Elizaga,A., Morrell,J., Bergen,A. and Penno,M.B.
  TITLE     Characterization of a gene coamplified with Ki-ras in Y1 murine
            adrenal carcinoma cells that codes for a putative membrane protein
  JOURNAL   Genomics 20 (2), 227-230 (1994)
   PUBMED   8020969
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022509.21.
            
            On Feb 27, 2020 this sequence version replaced NM_005086.4.
            
            Summary: This gene encodes a member of the dystrophin-glycoprotein
            complex (DGC). The DGC spans the sarcolemma and is comprised of
            dystrophin, syntrophin, alpha- and beta-dystroglycans and
            sarcoglycans. The DGC provides a structural link between the
            subsarcolemmal cytoskeleton and the extracellular matrix of muscle
            cells. Two alternatively spliced transcript variants that encode
            different protein isoforms have been described. [provided by
            RefSeq, Oct 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL136756.1, ERR4352441.170710.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000242729.7/ ENSP00000242729.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-379               AC022509.21        117391-117769
            380-466             AC022509.21        146111-146197
            467-4533            AC022509.21        152529-156595
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..4533
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="sarcospan"
                     /db_xref="GeneID:8082"
                     /db_xref="HGNC:HGNC:11322"
                     /db_xref="MIM:601599"
     exon            1..379
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..832
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     microspan; kirsten-ras-associated protein; K-ras
                     oncogene-associated protein; nanospan; sarcospan (Kras
                     oncogene-associated gene)"
                     /codon_start=1
                     /product="sarcospan isoform 1"
                     /protein_id="NP_005077.2"
                     /db_xref="CCDS:CCDS8707.1"
                     /db_xref="GeneID:8082"
                     /db_xref="HGNC:HGNC:11322"
                     /db_xref="MIM:601599"
                     /translation="MGKNKQPRGQQRQGGPPAADAAGPDDMEPKKGTGAPKECGEEEP
                     RTCCGCRFPLLLALLQLALGIAVTVVGFLMASISSSLLVRDTPFWAGIIVCLVAYLGL
                     FMLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLD
                     SCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWK
                     HRYQVFYVGVRICSLTASEGPQQKI"
     exon            380..466
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /inference="alignment:Splign:2.1.0"
     exon            467..4533
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4508..4513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="hexamer: AATAAA"
     polyA_site      4533
                     /gene="SSPN"
                     /gene_synonym="DAGA5; KRAG; NSPN; SPN1; SPN2"
                     /note="major polyA site"
ORIGIN      
        1 ataattcgaa taccagggca ggccgagcca gccgtgcgcc gcgctccagg gcccagggcg
       61 ccgcacacgc acccacccac ccacccagcc tcgcagcgcc atgggcaaga acaagcagcc
      121 acgcggccag cagaggcagg ggggcccgcc ggccgcggac gccgctgggc ccgacgacat
      181 ggagccgaag aagggcacgg gggcccccaa ggagtgcggg gaggaggagc cccggacctg
      241 ctgcggctgc cggttcccgc tgctgctcgc cctgctgcag ctggccctgg gcatcgccgt
      301 gaccgtggtg ggcttcctca tggcgagcat cagctcctcc ctgctagtca gggacactcc
      361 attttgggct gggatcattg tctgcttagt ggcctatctt ggcttgttta tgctttgtgt
      421 ctcatatcag gttgacgaac ggacatgtat tcaattttct atgaaactgt tatactttct
      481 gctgagtgcc ctgggcctga cggtctgtgt gctggccgtg gcctttgccg cccaccacta
      541 ttcgcagctc acacagttta cctgtgagac cacactcgac tcttgccagt gcaaactgcc
      601 ctcctcggag ccgctcagca ggacctttgt ttaccgggat gtgacggact gtaccagcgt
      661 cactggcact ttcaaactgt tcttactcat ccagatgatt cttaatttgg tctgcggcct
      721 tgtgtgcttg ttggcctgct ttgtgatgtg gaaacatagg taccaggtct tctatgtggg
      781 tgtcaggata tgctccctca cggcttccga aggcccccag caaaagatct aacattcttg
      841 ctcaaagttg cgagagaaag tagcacatgg agtagctgag gttaaacaaa caaaaaaaaa
      901 ttttaaacaa agaaaggaaa aaaattgaca ataaaagtca ctcttctaat tgaatatttt
      961 tatattttta tgaaacaaaa gagcatttct tcaggtttct attgtatttt ttttaacatt
     1021 cttgcagaga aagcaagatc caaattgatt ttgggatatt aaaagttaac agaacactga
     1081 acaaggaaag aatggcatag atctatcttt acagtctgga gttaattcct gttaactcat
     1141 tttatccatt ccttacataa tcttctttcc tgttagtcca gtttgatggt gtgaatggtg
     1201 aatttcaggc ccagttgcta aattttgtgg catcttcctc tagtccttcc cacctccagt
     1261 catcagcccc actctgtctt ggagacaggc aggaggtggg ggaagagctg aatctcttta
     1321 ttttccctgg tagagacatc ttcaaggcat gaaatagctt aaagagcaga gtagaaacgg
     1381 aagaggcttt gcaaaaggct agataactaa caacacctgg gttggggcgg cggcctcttc
     1441 tcttcagctc ccttagcttg gctccgtaag tggatcactt gccaaatgct ttagatgatt
     1501 gcctctcaat aattgaaagg tggtggtagt tgtattctaa atgatgtaga aggtttaaaa
     1561 ataattacat tatgcttcta ttctatcatc taaaacaaat cattaaaact aatttctagc
     1621 taattgttaa ttataattat gctcagaagt ctatttaatg agctctgact gtacttacgc
     1681 tgcactgtcg gtgttaagag aaattactct cacaagagca gaggcctgaa gattctttct
     1741 tctgaaagcc aagcaccaca aggaaaaaaa aaattattaa tagctcaggt taaaaacacc
     1801 catttaaaca aaaacaagag catttgtaat aggaagtgtt tatacaaaca gcacatttgt
     1861 gatatgttga aaagcatctc tcttggcaac caatctatgt ttgaggaaga ttgggtaatg
     1921 ctgatgtgtt ccattcatga aactgtattt gatacataat cctattatta attcgtatgc
     1981 ttagtcaacc taggaaatca aaataatgtt ttgaagttct tatttgagca atatggcctt
     2041 gacttggagg gtagttttag ttgttttgtt tttaagtgac tgtggtttaa agcacaaatg
     2101 ccccaaggtg gggagacttc tctctgtgat tattgttgct attaaattct gaactgtatc
     2161 catattttaa ggaaggagct aaaaatggaa attcatgaaa cataaatggt atcaagaact
     2221 ttatcagtat gctttgttga aagcagaaat taagataata attgagttca attcgcctct
     2281 ccgcattgcc tattgataca ctttactaat catgaaattc taacctaaaa ggaaaacatt
     2341 ttcctgcttg tcttagaaga aagtggaata attccactga ttgtgataat ggtttcaatt
     2401 tctacacaat ataaatatcc agtataaagg aaagcgttaa gtcggtaagc tagaggattg
     2461 taaatatctt ttatgtcctc tagataaaac acccgattaa cagatgttaa accttttaat
     2521 gttttgattt gctttaaaaa tggccttcct acacattagc tccagctaaa aagacacatt
     2581 ggagagctta gaggataagt ctctggagca gaatttatca cacacaaaag ttacaccaac
     2641 agaataccaa gcagaatgat gaggacctgt aaaatacctt gtgccctatt aaaaaaaaaa
     2701 aaaaaaaaaa agccagtaac tgaatccatt ttgatttttg gttgagtttc ctacacaaag
     2761 aagaaaataa ctgagaatct ggaatgttgt agtccatcct ttaaagagta agaaagtagc
     2821 agttaatgct agtaaccgtg aattaggcac cactgaaagc acatcccgaa tttctttaac
     2881 aacaacattt tatagtgaac actacaagtt tttatattta aaaattaaga ctctgtatat
     2941 ccttaaggtg ctctatgctt taccagtaat tcacagggta tttcaaatgg tagaatcatt
     3001 ttagcttctg tgcttccttt ttctaaataa tgcaacttgt aagagttgac attgtaataa
     3061 gctttataat agtataaccg tcaggagata tatatatata tatatacaca tacacacaca
     3121 cacacacata tatactatac atatataaaa tggggatatt actattgtat gattaaatca
     3181 ttcttaagtc cccaaggaaa aaaaatcata aacaaataga aagaactaaa cagaaaagaa
     3241 agaaagaaaa gatccctttg ctatgagctc gctgtatatt gataagtgta agaattgtgt
     3301 tgcatgaata accgctttgg gctggatttg aagtattttg atgttgtgtt ttgcaaatat
     3361 tgaagttaca ataatggcaa cagaaaagga acagatacac agctttacac ttagcagaat
     3421 gttacattta aaatctgaca aggcgccaag atggtgactg tctcctctaa accacgaaag
     3481 agtaagattt gtgcaaccct cctcctcttc cacctccttc aggagaatta aatgaatcaa
     3541 gactttggaa agacggggaa gagccgggac ttggcagtac ttgaaacagg aggaatacac
     3601 cagcctaaat gtacagactt tgtagccgag cccactcgat cggtctgtgc cttcacgtga
     3661 ccaccatctg tgcctccctc gctccatcca aatttgtgta ggctgctcct tggagctatg
     3721 cctaaaatat agctacacca gagccctgga aactgtagtc aagtaacagg cctcactgtt
     3781 ttttttcttt ggattaaaag tgtatatctc tctactgagg ggtttccagc tttacacttc
     3841 tataggttgt tctgacagtg agaacacatt atgtacatgt tttctattca tgtccagggc
     3901 atgagaatct taatattctt atacttccct ttttgcatgc agtatgtctg gaatatgttt
     3961 ataaaatttt agcaaggggt gagatcaagg gacaaaggta gagtggttat ttaaagacaa
     4021 tcagaaatga acttaaaagc tgccctgcaa aaccaatcct ttcctatcat gaagagtacc
     4081 ttcatatttt ctagagattg tctctgaacc gttgctacat agccataaat ttctgtaaat
     4141 catgttggct atcagttctt gctattctca gagcactcta tcatgttttt aggtgtatat
     4201 tgtgtattca ggatagcatt aaaaagaact gatggcaaat gtattgactc acagtggaaa
     4261 cattcatttg gatgaagaat tagtggttta gcctttgaaa tgaatacctt gaatcatttc
     4321 ttctgatttt ctagtagttt attatctcaa atgattatta tccccttcaa aaatactggt
     4381 ctgtactttg gtgttgtgga ttcatttttc tggcattgat caaatagcaa atacaaaata
     4441 aagcatttct acatgtttga ggtggttgtt tttaaagggg atatgtgtaa tgcagatttt
     4501 ctttattaat aaaattttca taatctctga aaa
//
